AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045145.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045145.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880045145.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0880045145.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045145.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880045145.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 28 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGCCTGCGATTCGTCGTT+TGG 0.336158 8:+54910131 MsG0880045145.01.T01:CDS
GGGAGAACTCTTTACGGTTT+CGG 0.376970 8:+54910321 MsG0880045145.01.T01:CDS
TCGCAGGCTTTGTTATTCCT+TGG 0.407291 8:-54910119 None:intergenic
AAAATGTCAGGTCGCGGAAA+AGG 0.432832 8:+54910036 None:intergenic
AGACAGGGGAGAACTCTTTA+CGG 0.442868 8:+54910315 MsG0880045145.01.T01:CDS
GGAAAAGGAGGAAAGGGATT+AGG 0.448451 8:+54910051 MsG0880045145.01.T01:CDS
TGTTTATGCTTTGAAGAGAC+AGG 0.461410 8:+54910299 MsG0880045145.01.T01:CDS
AGGAGCAAAGCGCCACAGAA+AGG 0.515503 8:+54910080 MsG0880045145.01.T01:CDS
CGTCGTTTGGCTCGAAGAGG+AGG 0.516708 8:+54910144 MsG0880045145.01.T01:CDS
TTACTTACACTGAGCATGCT+AGG 0.518049 8:+54910250 MsG0880045145.01.T01:CDS
GGAGGAAAGGGATTAGGAAA+AGG 0.522854 8:+54910057 MsG0880045145.01.T01:CDS
CGAGCCAAACGACGAATCGC+AGG 0.562444 8:-54910135 None:intergenic
GGAAAGGGATTAGGAAAAGG+AGG 0.569746 8:+54910060 MsG0880045145.01.T01:CDS
GGTCGCGGAAAAGGAGGAAA+GGG 0.574670 8:+54910045 MsG0880045145.01.T01:CDS
ATGTCAGGTCGCGGAAAAGG+AGG 0.578066 8:+54910039 MsG0880045145.01.T01:CDS
GAGGAAAACTGTTACCGCCA+TGG 0.588179 8:+54910272 MsG0880045145.01.T01:CDS
GTTTATGCTTTGAAGAGACA+GGG 0.590468 8:+54910300 MsG0880045145.01.T01:CDS
GGAGGTGTGAAGAGAATCAG+TGG 0.594114 8:+54910162 MsG0880045145.01.T01:CDS
AGGTCGCGGAAAAGGAGGAA+AGG 0.594714 8:+54910044 MsG0880045145.01.T01:CDS
AGAACTCTTTACGGTTTCGG+TGG 0.599890 8:+54910324 MsG0880045145.01.T01:CDS
CAAAGCATAAACAACATCCA+TGG 0.612707 8:-54910289 None:intergenic
ATTCGTCGTTTGGCTCGAAG+AGG 0.614299 8:+54910141 MsG0880045145.01.T01:CDS
GTTCTTCGTGACAACATCCA+AGG 0.625870 8:+54910102 MsG0880045145.01.T01:CDS
TTTATGCTTTGAAGAGACAG+GGG 0.664122 8:+54910301 MsG0880045145.01.T01:CDS
CTTATCTATGAAGAAACTCG+CGG 0.683519 8:+54910186 MsG0880045145.01.T01:CDS
TGTCACGAAGAACCTTTCTG+TGG 0.693218 8:-54910092 None:intergenic
CTTACACTGAGCATGCTAGG+AGG 0.700541 8:+54910253 MsG0880045145.01.T01:CDS
AGCATAAACAACATCCATGG+CGG 0.725411 8:-54910286 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CAAAGCATAAACAACATCCA+TGG - Chr8:54910292-54910311 None:intergenic 35.0%
CTTATCTATGAAGAAACTCG+CGG + Chr8:54910186-54910205 MsG0880045145.01.T01:CDS 35.0%
!! GTTTATGCTTTGAAGAGACA+GGG + Chr8:54910300-54910319 MsG0880045145.01.T01:CDS 35.0%
!! TGTTTATGCTTTGAAGAGAC+AGG + Chr8:54910299-54910318 MsG0880045145.01.T01:CDS 35.0%
!! TTTATGCTTTGAAGAGACAG+GGG + Chr8:54910301-54910320 MsG0880045145.01.T01:CDS 35.0%
AGCATAAACAACATCCATGG+CGG - Chr8:54910289-54910308 None:intergenic 40.0%
TTACTTACACTGAGCATGCT+AGG + Chr8:54910250-54910269 MsG0880045145.01.T01:CDS 40.0%
AGAACTCTTTACGGTTTCGG+TGG + Chr8:54910324-54910343 MsG0880045145.01.T01:CDS 45.0%
AGACAGGGGAGAACTCTTTA+CGG + Chr8:54910315-54910334 MsG0880045145.01.T01:CDS 45.0%
GGAAAAGGAGGAAAGGGATT+AGG + Chr8:54910051-54910070 MsG0880045145.01.T01:CDS 45.0%
GGAAAGGGATTAGGAAAAGG+AGG + Chr8:54910060-54910079 MsG0880045145.01.T01:CDS 45.0%
GGAGGAAAGGGATTAGGAAA+AGG + Chr8:54910057-54910076 MsG0880045145.01.T01:CDS 45.0%
GGGAGAACTCTTTACGGTTT+CGG + Chr8:54910321-54910340 MsG0880045145.01.T01:CDS 45.0%
GTTCTTCGTGACAACATCCA+AGG + Chr8:54910102-54910121 MsG0880045145.01.T01:CDS 45.0%
TGTCACGAAGAACCTTTCTG+TGG - Chr8:54910095-54910114 None:intergenic 45.0%
!! TCGCAGGCTTTGTTATTCCT+TGG - Chr8:54910122-54910141 None:intergenic 45.0%
AAAGCCTGCGATTCGTCGTT+TGG + Chr8:54910131-54910150 MsG0880045145.01.T01:CDS 50.0%
CTTACACTGAGCATGCTAGG+AGG + Chr8:54910253-54910272 MsG0880045145.01.T01:CDS 50.0%
GAGGAAAACTGTTACCGCCA+TGG + Chr8:54910272-54910291 MsG0880045145.01.T01:CDS 50.0%
! ATTCGTCGTTTGGCTCGAAG+AGG + Chr8:54910141-54910160 MsG0880045145.01.T01:CDS 50.0%
! GGAGGTGTGAAGAGAATCAG+TGG + Chr8:54910162-54910181 MsG0880045145.01.T01:CDS 50.0%
AGGTCGCGGAAAAGGAGGAA+AGG + Chr8:54910044-54910063 MsG0880045145.01.T01:CDS 55.0%
ATGTCAGGTCGCGGAAAAGG+AGG + Chr8:54910039-54910058 MsG0880045145.01.T01:CDS 55.0%
GGTCGCGGAAAAGGAGGAAA+GGG + Chr8:54910045-54910064 MsG0880045145.01.T01:CDS 55.0%
! AGGAGCAAAGCGCCACAGAA+AGG + Chr8:54910080-54910099 MsG0880045145.01.T01:CDS 55.0%
CGAGCCAAACGACGAATCGC+AGG - Chr8:54910138-54910157 None:intergenic 60.0%
! CGTCGTTTGGCTCGAAGAGG+AGG + Chr8:54910144-54910163 MsG0880045145.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 54910039 54910350 54910039 ID=MsG0880045145.01;Name=MsG0880045145.01
Chr8 mRNA 54910039 54910350 54910039 ID=MsG0880045145.01.T01;Parent=MsG0880045145.01;Name=MsG0880045145.01.T01;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|103
Chr8 exon 54910039 54910350 54910039 ID=MsG0880045145.01.T01:exon:23683;Parent=MsG0880045145.01.T01
Chr8 CDS 54910039 54910350 54910039 ID=MsG0880045145.01.T01:cds;Parent=MsG0880045145.01.T01
Gene Sequence

>MsG0880045145.01.T01

ATGTCAGGTCGCGGAAAAGGAGGAAAGGGATTAGGAAAAGGAGGAGCAAAGCGCCACAGAAAGGTTCTTCGTGACAACATCCAAGGAATAACAAAGCCTGCGATTCGTCGTTTGGCTCGAAGAGGAGGTGTGAAGAGAATCAGTGGTCTTATCTATGAAGAAACTCGCGGTGTTCTCAAGATCTTTCTTGAGAACGTTATTCGTGATGCTGTTACTTACACTGAGCATGCTAGGAGGAAAACTGTTACCGCCATGGATGTTGTTTATGCTTTGAAGAGACAGGGGAGAACTCTTTACGGTTTCGGTGGTTGA

Protein sequence

>MsG0880045145.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*