Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040545.01.T01 | XP_003625191.1 | 80 | 145 | 5 | 3 | 1 | 121 | 1 | 145 | 1.14E-52 | 172 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047791.01 | MsG0780040545.01 | 0.820102 | 8.187807e-53 | 6.419447e-50 |
| MsG0080047944.01 | MsG0780040545.01 | 0.819079 | 1.402299e-52 | 1.068377e-49 |
| MsG0080048383.01 | MsG0780040545.01 | 0.806358 | 8.549519e-50 | 4.630905e-47 |
| MsG0080048462.01 | MsG0780040545.01 | 0.809280 | 2.045375e-50 | 1.195879e-47 |
| MsG0080048856.01 | MsG0780040545.01 | 0.805950 | 1.042096e-49 | 5.586328e-47 |
| MsG0080048964.01 | MsG0780040545.01 | 0.800159 | 1.642199e-48 | 7.592286e-46 |
| MsG0080049121.01 | MsG0780040545.01 | 0.816976 | 4.188060e-52 | 3.011866e-49 |
| MsG0180000139.01 | MsG0780040545.01 | 0.815047 | 1.128925e-51 | 7.700416e-49 |
| MsG0180000205.01 | MsG0780040545.01 | 0.815991 | 6.960050e-52 | 4.871900e-49 |
| MsG0180000284.01 | MsG0780040545.01 | 0.805849 | 1.094361e-49 | 5.851590e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780040545.01.T01 | MTR_7g092450 | 80.000 | 145 | 5 | 3 | 1 | 121 | 1 | 145 | 1.38e-56 | 172 |
| MsG0780040545.01.T01 | MTR_0019s0160 | 64.138 | 145 | 27 | 4 | 1 | 121 | 1 | 144 | 5.11e-43 | 138 |
Find 66 sgRNAs with CRISPR-Local
Find 296 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACGATGCAGGTACGAGG+AGG | 0.694919 | 7:+79011667 | MsG0780040545.01.T01:intron |
| AAATACACACCAAAACTAAT+AGG | 0.342786 | 7:-79015038 | None:intergenic |
| AACTCTTCAGATTGCCATGA+AGG | 0.418174 | 7:+79014648 | MsG0780040545.01.T01:intron |
| AAGTAGCGACGGATGGTAAA+AGG | 0.371530 | 7:-79014693 | None:intergenic |
| AAGTATGGTACCTCAATTTC+AGG | 0.283991 | 7:-79011701 | None:intergenic |
| AATGATGACCTAGATGCAGA+AGG | 0.620982 | 7:+79011814 | MsG0780040545.01.T01:CDS |
| AATTGTCCAGCTATGAAGAT+TGG | 0.435283 | 7:+79014834 | MsG0780040545.01.T01:intron |
| ACCAATTGAAACTGAGGAAA+AGG | 0.424157 | 7:+79011840 | MsG0780040545.01.T01:CDS |
| ACCACCCACTTGTCTCTTCC+AGG | 0.359364 | 7:-79014885 | None:intergenic |
| ACCTCAATTTCAGGCTCTGG+TGG | 0.507724 | 7:-79011692 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATGGTTAAATTACCTCAAG+TGG | - | Chr7:79013444-79013463 | None:intergenic | 30.0% | |
| ACAGTTGATTGGACAAAAAT+AGG | - | Chr7:79015155-79015174 | None:intergenic | 30.0% | |
| ACATGTATGTCAGCATATAT+TGG | - | Chr7:79013745-79013764 | None:intergenic | 30.0% | |
| ATACAAACAAGATCAACATC+TGG | - | Chr7:79013685-79013704 | None:intergenic | 30.0% | |
| ATCAATGGATGATGTAAAAC+AGG | - | Chr7:79012153-79012172 | None:intergenic | 30.0% | |
| ATCTGACCACATGTTAATTT+AGG | + | Chr7:79014148-79014167 | MsG0780040545.01.T01:intron | 30.0% | |
| ATCTTAAACAATCATACTGC+AGG | + | Chr7:79013334-79013353 | MsG0780040545.01.T01:intron | 30.0% | |
| ATGGTTAAATTACCTCAAGT+GGG | - | Chr7:79013443-79013462 | None:intergenic | 30.0% | |
| ATTAATTATGTGAGTGAGAG+AGG | - | Chr7:79011584-79011603 | None:intergenic | 30.0% | |
| ATTGCAATATGAACCTTACA+AGG | - | Chr7:79012957-79012976 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 79011459 | 79015237 | 79011459 | ID=MsG0780040545.01;Name=MsG0780040545.01 |
| Chr7 | mRNA | 79011459 | 79015237 | 79011459 | ID=MsG0780040545.01.T01;Parent=MsG0780040545.01;Name=MsG0780040545.01.T01;_AED=0.42;_eAED=0.42;_QI=71|0.75|0.8|1|1|1|5|324|121 |
| Chr7 | exon | 79011459 | 79011564 | 79011459 | ID=MsG0780040545.01.T01:exon:16186;Parent=MsG0780040545.01.T01 |
| Chr7 | exon | 79011679 | 79011712 | 79011679 | ID=MsG0780040545.01.T01:exon:16187;Parent=MsG0780040545.01.T01 |
| Chr7 | exon | 79011781 | 79011937 | 79011781 | ID=MsG0780040545.01.T01:exon:16188;Parent=MsG0780040545.01.T01 |
| Chr7 | exon | 79014658 | 79014727 | 79014658 | ID=MsG0780040545.01.T01:exon:16189;Parent=MsG0780040545.01.T01 |
| Chr7 | exon | 79014844 | 79015237 | 79014844 | ID=MsG0780040545.01.T01:exon:16190;Parent=MsG0780040545.01.T01 |
| Chr7 | five_prime_UTR | 79011459 | 79011529 | 79011459 | ID=MsG0780040545.01.T01:five_prime_utr;Parent=MsG0780040545.01.T01 |
| Chr7 | CDS | 79011530 | 79011564 | 79011530 | ID=MsG0780040545.01.T01:cds;Parent=MsG0780040545.01.T01 |
| Chr7 | CDS | 79011679 | 79011712 | 79011679 | ID=MsG0780040545.01.T01:cds;Parent=MsG0780040545.01.T01 |
| Chr7 | CDS | 79011781 | 79011937 | 79011781 | ID=MsG0780040545.01.T01:cds;Parent=MsG0780040545.01.T01 |
| Chr7 | CDS | 79014658 | 79014727 | 79014658 | ID=MsG0780040545.01.T01:cds;Parent=MsG0780040545.01.T01 |
| Chr7 | CDS | 79014844 | 79014913 | 79014844 | ID=MsG0780040545.01.T01:cds;Parent=MsG0780040545.01.T01 |
| Chr7 | three_prime_UTR | 79014914 | 79015237 | 79014914 | ID=MsG0780040545.01.T01:three_prime_utr;Parent=MsG0780040545.01.T01 |
| Gene Sequence |
>MsG0780040545.01.T01 ATGGCCGACGATGACTACGAAGATATCGACATGGGGTACGAGGAGGAGCCACCAGAGCCTGAAATTGAGGAAGGTGCAGAGGAGGAGGATCCTGATAACAAGAATGATGACCTAGATGCAGAAGGAGAACCAATTGAAACTGAGGAAAAGGAAGATGCTGCTCCGGTTGAACGTCCCAGAAGGACTTCCAAGTATATGACTAAGTATGAGCGTGCTAGGATTCTCGATTGCCATGAAGGAGCTAGACTACGCGAGAAAATTCCTTTTACCATCCGTCGCTACTTGCCTGATGGAAGCTATGAAGATTGGGGAGTTGATGAGCTGATTGTGGAAGACTCCTGGAAGAGACAAGTGGGTGGTGATTGA |
| Protein sequence |
>MsG0780040545.01.T01 MADDDYEDIDMGYEEEPPEPEIEEGAEEEDPDNKNDDLDAEGEPIETEEKEDAAPVERPRRTSKYMTKYERARILDCHEGARLREKIPFTIRRYLPDGSYEDWGVDELIVEDSWKRQVGGD* |
