AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880047472.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047472.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0880047472.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0880047472.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880047472.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0880047472.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 32 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGAAGGACTCTTTA+TGG 0.247348 8:+86874106 MsG0880047472.01.T01:CDS
GGAAGGACTCTTTATGGATT+TGG 0.297351 8:+86874112 MsG0880047472.01.T01:CDS
TCCTTTGCCTCTTCCTGACA+TGG 0.418595 8:-86873828 None:intergenic
TCTTTATGGATTTGGCGGTT+AGG 0.439485 8:+86874120 MsG0880047472.01.T01:CDS
GGCAAAGGAGGAAAGGGTCT+TGG 0.446329 8:+86873842 MsG0880047472.01.T01:CDS
CAAGAATTCACAGCCATGTC+AGG 0.481900 8:+86873815 None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG 0.488613 8:+86874090 MsG0880047472.01.T01:CDS
GGTTCTTCGCGATAACATCC+AGG 0.500175 8:+86873892 MsG0880047472.01.T01:CDS
AGGACTCTTTATGGATTTGG+CGG 0.509079 8:+86874115 MsG0880047472.01.T01:CDS
GGAGGAAAGGGTCTTGGAAA+GGG 0.511441 8:+86873848 MsG0880047472.01.T01:CDS
TCGCAGGCTTCGTGATTCCC+TGG 0.511861 8:-86873910 None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 8:+86873835 MsG0880047472.01.T01:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 8:+86873836 MsG0880047472.01.T01:CDS
CCATCGCAGTAACAGTCTTA+CGG 0.545551 8:-86874063 None:intergenic
GCCATGTCAGGAAGAGGCAA+AGG 0.547501 8:+86873827 None:intergenic
AGGAGGAAAGGGTCTTGGAA+AGG 0.553664 8:+86873847 MsG0880047472.01.T01:CDS
CCGTAAGACTGTTACTGCGA+TGG 0.561095 8:+86874063 MsG0880047472.01.T01:CDS
CTTGCGAGACGACGAATCGC+AGG 0.571080 8:-86873926 None:intergenic
TTCACAGCCATGTCAGGAAG+AGG 0.573281 8:+86873821 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 8:+86873871 MsG0880047472.01.T01:CDS
TGTTATCGCGAAGAACCTTA+CGG 0.576355 8:-86873886 None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG 0.581057 8:+86873830 MsG0880047472.01.T01:CDS
ACGCTCTCAAGAGGCAGGGA+AGG 0.583359 8:+86874095 MsG0880047472.01.T01:CDS
ACGGCGAGCATGCTCTGTGT+AGG 0.590446 8:-86874044 None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG 0.621886 8:+86873851 MsG0880047472.01.T01:CDS
ATTCGTCGTCTCGCAAGACG+TGG 0.624267 8:+86873932 MsG0880047472.01.T01:CDS
TTAATCTATGAAGAAACTCG+TGG 0.634992 8:+86873977 MsG0880047472.01.T01:CDS
ATGTCGTTTACGCTCTCAAG+AGG 0.636714 8:+86874086 MsG0880047472.01.T01:CDS
CGTCGTCTCGCAAGACGTGG+TGG 0.642630 8:+86873935 MsG0880047472.01.T01:CDS
GTTCTTCGCGATAACATCCA+GGG 0.657095 8:+86873893 MsG0880047472.01.T01:CDS
GTTTACGCTCTCAAGAGGCA+GGG 0.663401 8:+86874091 MsG0880047472.01.T01:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.669421 8:+86873953 MsG0880047472.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG + Chr8:86873977-86873996 MsG0880047472.01.T01:CDS 30.0%
AGGACTCTTTATGGATTTGG+CGG + Chr8:86874115-86874134 MsG0880047472.01.T01:CDS 40.0%
GGAAGGACTCTTTATGGATT+TGG + Chr8:86874112-86874131 MsG0880047472.01.T01:CDS 40.0%
TGTTATCGCGAAGAACCTTA+CGG - Chr8:86873889-86873908 None:intergenic 40.0%
CCATCGCAGTAACAGTCTTA+CGG - Chr8:86874066-86874085 None:intergenic 45.0%
GTTCTTCGCGATAACATCCA+GGG + Chr8:86873893-86873912 MsG0880047472.01.T01:CDS 45.0%
! ATGTCGTTTACGCTCTCAAG+AGG + Chr8:86874086-86874105 MsG0880047472.01.T01:CDS 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG + Chr8:86873835-86873854 MsG0880047472.01.T01:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG + Chr8:86873871-86873890 MsG0880047472.01.T01:CDS 50.0%
AGGAGGAAAGGGTCTTGGAA+AGG + Chr8:86873847-86873866 MsG0880047472.01.T01:CDS 50.0%
AGGCAGGGAAGGACTCTTTA+TGG + Chr8:86874106-86874125 MsG0880047472.01.T01:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG + Chr8:86873830-86873849 MsG0880047472.01.T01:CDS 50.0%
CCGTAAGACTGTTACTGCGA+TGG + Chr8:86874063-86874082 MsG0880047472.01.T01:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG + Chr8:86873836-86873855 MsG0880047472.01.T01:CDS 50.0%
GGAGGAAAGGGTCTTGGAAA+GGG + Chr8:86873848-86873867 MsG0880047472.01.T01:CDS 50.0%
GGTTCTTCGCGATAACATCC+AGG + Chr8:86873892-86873911 MsG0880047472.01.T01:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG + Chr8:86874091-86874110 MsG0880047472.01.T01:CDS 50.0%
ATTCGTCGTCTCGCAAGACG+TGG + Chr8:86873932-86873951 MsG0880047472.01.T01:CDS 55.0%
CGTTTACGCTCTCAAGAGGC+AGG + Chr8:86874090-86874109 MsG0880047472.01.T01:CDS 55.0%
GGAAAGGGTCTTGGAAAGGG+AGG + Chr8:86873851-86873870 MsG0880047472.01.T01:CDS 55.0%
GGCAAAGGAGGAAAGGGTCT+TGG + Chr8:86873842-86873861 MsG0880047472.01.T01:CDS 55.0%
GGTGGTGTGAAGCGTATCAG+TGG + Chr8:86873953-86873972 MsG0880047472.01.T01:CDS 55.0%
ACGCTCTCAAGAGGCAGGGA+AGG + Chr8:86874095-86874114 MsG0880047472.01.T01:CDS 60.0%
ACGGCGAGCATGCTCTGTGT+AGG - Chr8:86874047-86874066 None:intergenic 60.0%
CTTGCGAGACGACGAATCGC+AGG - Chr8:86873929-86873948 None:intergenic 60.0%
TCGCAGGCTTCGTGATTCCC+TGG - Chr8:86873913-86873932 None:intergenic 60.0%
CGTCGTCTCGCAAGACGTGG+TGG + Chr8:86873935-86873954 MsG0880047472.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 86873830 86874141 86873830 ID=MsG0880047472.01;Name=MsG0880047472.01
Chr8 mRNA 86873830 86874141 86873830 ID=MsG0880047472.01.T01;Parent=MsG0880047472.01;Name=MsG0880047472.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103
Chr8 exon 86873830 86874141 86873830 ID=MsG0880047472.01.T01:exon:37415;Parent=MsG0880047472.01.T01
Chr8 CDS 86873830 86874141 86873830 ID=MsG0880047472.01.T01:cds;Parent=MsG0880047472.01.T01
Gene Sequence

>MsG0880047472.01.T01

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG

Protein sequence

>MsG0880047472.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*