AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780037240.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037240.01.T01 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.97e-68 199
MsG0780037240.01.T01 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.74e-66 204
MsG0780037240.01.T01 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.23e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780037240.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT5G59690 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT5G59970 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT3G46320 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT3G45930 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT3G53730 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT2G28740 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT1G07660 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT1G07820 100.000 103 0 0 1 103 1 103 3.11e-68 199
MsG0780037240.01.T01 AT1G07660 83.495 103 0 1 1 103 1 86 4.52e-51 155

Find 32 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCTTCCCTGTCTCTTA+AGG 0.300277 7:-22294579 None:intergenic
AGGAGTGTTGAAGATCTTCT+TGG 0.386764 7:+22294477 MsG0780037240.01.T01:CDS
TCGCTGGCTTTGTGATTCCT+TGG 0.395127 7:-22294390 None:intergenic
GGTTCTTCCCTGTCTCTTAA+GGG 0.396932 7:-22294578 None:intergenic
AATCAGCGGTTTGATTTATG+AGG 0.401936 7:+22294447 MsG0780037240.01.T01:CDS
GGAAAGGGAGGTAAAGGATT+AGG 0.430594 7:+22294322 MsG0780037240.01.T01:CDS
AGACAGGGAAGAACCCTCTA+TGG 0.440459 7:+22294586 MsG0780037240.01.T01:CDS
GGAAGAACCCTCTATGGTTT+CGG 0.453170 7:+22294592 MsG0780037240.01.T01:CDS
TGTTTATGCCCTTAAGAGAC+AGG 0.454713 7:+22294570 MsG0780037240.01.T01:CDS
AGTCTTCCTCCTCGCATGCT+CGG 0.461551 7:-22294530 None:intergenic
CTCGCCAATCGACGAATCGC+TGG 0.484651 7:-22294406 None:intergenic
TTCAACCACCGAAACCATAG+AGG 0.495992 7:-22294600 None:intergenic
AAAATGTCTGGTCGTGGAAA+GGG 0.502849 7:+22294307 None:intergenic
AAAGCCAGCGATTCGTCGAT+TGG 0.503407 7:+22294402 MsG0780037240.01.T01:CDS
CGTCGATTGGCGAGAAGAGG+AGG 0.518547 7:+22294415 MsG0780037240.01.T01:CDS
GGTCGTGGAAAGGGAGGTAA+AGG 0.529255 7:+22294316 MsG0780037240.01.T01:CDS
GAGGAAGACTGTGACTGCTA+TGG 0.530595 7:+22294543 MsG0780037240.01.T01:CDS
GGAGGTAAAGGATTAGGAAA+GGG 0.542976 7:+22294328 MsG0780037240.01.T01:CDS
GGGAGGTAAAGGATTAGGAA+AGG 0.547562 7:+22294327 MsG0780037240.01.T01:CDS
TTGATTTATGAGGAAACAAG+AGG 0.572644 7:+22294457 MsG0780037240.01.T01:CDS
ATTCGTCGATTGGCGAGAAG+AGG 0.594887 7:+22294412 MsG0780037240.01.T01:CDS
GGTAAAGGATTAGGAAAGGG+AGG 0.605777 7:+22294331 MsG0780037240.01.T01:CDS
AGAACCCTCTATGGTTTCGG+TGG 0.607847 7:+22294595 MsG0780037240.01.T01:CDS
TTACTTACACCGAGCATGCG+AGG 0.610843 7:+22294521 MsG0780037240.01.T01:CDS
GTTCTCCGCGACAACATCCA+AGG 0.619358 7:+22294373 MsG0780037240.01.T01:CDS
TGATTCCTTGGATGTTGTCG+CGG 0.639365 7:-22294378 None:intergenic
ATGTCTGGTCGTGGAAAGGG+AGG 0.640958 7:+22294310 MsG0780037240.01.T01:CDS
GTTTATGCCCTTAAGAGACA+GGG 0.645697 7:+22294571 MsG0780037240.01.T01:CDS
AGGTGCAAAGAGACATCGCA+AGG 0.665418 7:+22294351 MsG0780037240.01.T01:CDS
TCAACCACCGAAACCATAGA+GGG 0.678652 7:-22294599 None:intergenic
GGAGGTGTGAAAAGAATCAG+CGG 0.710972 7:+22294433 MsG0780037240.01.T01:CDS
CTTACACCGAGCATGCGAGG+AGG 0.753233 7:+22294524 MsG0780037240.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTGATTTATGAGGAAACAAG+AGG + Chr7:22294457-22294476 MsG0780037240.01.T01:CDS 30.0%
! AATCAGCGGTTTGATTTATG+AGG + Chr7:22294447-22294466 MsG0780037240.01.T01:CDS 35.0%
GGAGGTAAAGGATTAGGAAA+GGG + Chr7:22294328-22294347 MsG0780037240.01.T01:CDS 40.0%
GTTTATGCCCTTAAGAGACA+GGG + Chr7:22294571-22294590 MsG0780037240.01.T01:CDS 40.0%
TGTTTATGCCCTTAAGAGAC+AGG + Chr7:22294570-22294589 MsG0780037240.01.T01:CDS 40.0%
!! AGGAGTGTTGAAGATCTTCT+TGG + Chr7:22294477-22294496 MsG0780037240.01.T01:CDS 40.0%
GGAAAGGGAGGTAAAGGATT+AGG + Chr7:22294322-22294341 MsG0780037240.01.T01:CDS 45.0%
GGAAGAACCCTCTATGGTTT+CGG + Chr7:22294592-22294611 MsG0780037240.01.T01:CDS 45.0%
GGGAGGTAAAGGATTAGGAA+AGG + Chr7:22294327-22294346 MsG0780037240.01.T01:CDS 45.0%
GGTAAAGGATTAGGAAAGGG+AGG + Chr7:22294331-22294350 MsG0780037240.01.T01:CDS 45.0%
GGTTCTTCCCTGTCTCTTAA+GGG - Chr7:22294581-22294600 None:intergenic 45.0%
TCAACCACCGAAACCATAGA+GGG - Chr7:22294602-22294621 None:intergenic 45.0%
TGATTCCTTGGATGTTGTCG+CGG - Chr7:22294381-22294400 None:intergenic 45.0%
! GGAGGTGTGAAAAGAATCAG+CGG + Chr7:22294433-22294452 MsG0780037240.01.T01:CDS 45.0%
AAAGCCAGCGATTCGTCGAT+TGG + Chr7:22294402-22294421 MsG0780037240.01.T01:CDS 50.0%
AGAACCCTCTATGGTTTCGG+TGG + Chr7:22294595-22294614 MsG0780037240.01.T01:CDS 50.0%
AGACAGGGAAGAACCCTCTA+TGG + Chr7:22294586-22294605 MsG0780037240.01.T01:CDS 50.0%
AGGTGCAAAGAGACATCGCA+AGG + Chr7:22294351-22294370 MsG0780037240.01.T01:CDS 50.0%
GAGGAAGACTGTGACTGCTA+TGG + Chr7:22294543-22294562 MsG0780037240.01.T01:CDS 50.0%
GGGTTCTTCCCTGTCTCTTA+AGG - Chr7:22294582-22294601 None:intergenic 50.0%
TTACTTACACCGAGCATGCG+AGG + Chr7:22294521-22294540 MsG0780037240.01.T01:CDS 50.0%
! ATTCGTCGATTGGCGAGAAG+AGG + Chr7:22294412-22294431 MsG0780037240.01.T01:CDS 50.0%
!! TCGCTGGCTTTGTGATTCCT+TGG - Chr7:22294393-22294412 None:intergenic 50.0%
AGTCTTCCTCCTCGCATGCT+CGG - Chr7:22294533-22294552 None:intergenic 55.0%
ATGTCTGGTCGTGGAAAGGG+AGG + Chr7:22294310-22294329 MsG0780037240.01.T01:CDS 55.0%
GGTCGTGGAAAGGGAGGTAA+AGG + Chr7:22294316-22294335 MsG0780037240.01.T01:CDS 55.0%
GTTCTCCGCGACAACATCCA+AGG + Chr7:22294373-22294392 MsG0780037240.01.T01:CDS 55.0%
CTCGCCAATCGACGAATCGC+TGG - Chr7:22294409-22294428 None:intergenic 60.0%
CTTACACCGAGCATGCGAGG+AGG + Chr7:22294524-22294543 MsG0780037240.01.T01:CDS 60.0%
! CGTCGATTGGCGAGAAGAGG+AGG + Chr7:22294415-22294434 MsG0780037240.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 22294310 22294621 22294310 ID=MsG0780037240.01;Name=MsG0780037240.01
Chr7 mRNA 22294310 22294621 22294310 ID=MsG0780037240.01.T01;Parent=MsG0780037240.01;Name=MsG0780037240.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|103
Chr7 exon 22294310 22294621 22294310 ID=MsG0780037240.01.T01:exon:32060;Parent=MsG0780037240.01.T01
Chr7 CDS 22294310 22294621 22294310 ID=MsG0780037240.01.T01:cds;Parent=MsG0780037240.01.T01
Gene Sequence

>MsG0780037240.01.T01

ATGTCTGGTCGTGGAAAGGGAGGTAAAGGATTAGGAAAGGGAGGTGCAAAGAGACATCGCAAGGTTCTCCGCGACAACATCCAAGGAATCACAAAGCCAGCGATTCGTCGATTGGCGAGAAGAGGAGGTGTGAAAAGAATCAGCGGTTTGATTTATGAGGAAACAAGAGGAGTGTTGAAGATCTTCTTGGAGAATGTGATAAGAGATGCTGTTACTTACACCGAGCATGCGAGGAGGAAGACTGTGACTGCTATGGATGTTGTTTATGCCCTTAAGAGACAGGGAAGAACCCTCTATGGTTTCGGTGGTTGA

Protein sequence

>MsG0780037240.01.T01

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG*