Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047084.01.T01 | XP_013458291.3 | 98.734 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 1.92E-106 | 310 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047084.01.T01 | Q06930 | 80.38 | 158 | 31 | 0 | 1 | 158 | 1 | 158 | 6.55E-89 | 259 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047084.01.T01 | A0A396IFH7 | 98.734 | 158 | 2 | 0 | 1 | 158 | 1 | 158 | 9.15e-107 | 310 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880047084.01 | MsG0880047085.01 | 0.948605 | 6.774308e-107 | 1.276430e-101 |
| MsG0180005372.01 | MsG0880047084.01 | 0.838199 | 3.314671e-57 | 4.407112e-54 |
| MsG0780041317.01 | MsG0880047084.01 | 0.808086 | 3.680643e-50 | 2.085098e-47 |
| MsG0880042591.01 | MsG0880047084.01 | 0.842616 | 2.319496e-58 | 3.536426e-55 |
| MsG0880043669.01 | MsG0880047084.01 | 0.850092 | 2.122829e-60 | 4.111641e-57 |
| MsG0280011203.01 | MsG0880047084.01 | 0.806783 | 6.955294e-50 | 3.808634e-47 |
| MsG0480019217.01 | MsG0880047084.01 | 0.816936 | 4.276335e-52 | 3.071904e-49 |
| MsG0580024591.01 | MsG0880047084.01 | 0.803895 | 2.802123e-49 | 1.424854e-46 |
| MsG0580026019.01 | MsG0880047084.01 | 0.825897 | 3.648732e-54 | 3.369743e-51 |
| MsG0680030613.01 | MsG0880047084.01 | 0.826146 | 3.183693e-54 | 2.960989e-51 |
| MsG0280007911.01 | MsG0880047084.01 | 0.848727 | 5.096576e-60 | 9.442267e-57 |
| MsG0780036661.01 | MsG0880047084.01 | 0.821365 | 4.197136e-53 | 3.407639e-50 |
| MsG0780036726.01 | MsG0880047084.01 | 0.822589 | 2.184601e-53 | 1.836632e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047084.01.T01 | MTR_6g033450 | 97.468 | 158 | 4 | 0 | 1 | 158 | 1 | 158 | 4.54e-109 | 307 |
| MsG0880047084.01.T01 | MTR_4g120970 | 97.468 | 158 | 4 | 0 | 1 | 158 | 1 | 158 | 4.54e-109 | 307 |
| MsG0880047084.01.T01 | MTR_2g035150 | 74.684 | 158 | 40 | 0 | 1 | 158 | 1 | 158 | 7.51e-83 | 241 |
| MsG0880047084.01.T01 | MTR_2g035100 | 73.248 | 157 | 42 | 0 | 1 | 157 | 1 | 157 | 2.45e-81 | 237 |
| MsG0880047084.01.T01 | MTR_2g035105 | 72.611 | 157 | 43 | 0 | 1 | 157 | 1 | 157 | 2.24e-80 | 234 |
| MsG0880047084.01.T01 | MTR_2g035130 | 70.701 | 157 | 46 | 0 | 1 | 157 | 1 | 157 | 3.92e-79 | 231 |
| MsG0880047084.01.T01 | MTR_2g035120 | 68.987 | 158 | 49 | 0 | 1 | 158 | 1 | 158 | 1.08e-76 | 225 |
| MsG0880047084.01.T01 | MTR_2g035320 | 63.694 | 157 | 57 | 0 | 1 | 157 | 1 | 157 | 2.94e-68 | 204 |
| MsG0880047084.01.T01 | MTR_2g035210 | 64.331 | 157 | 56 | 0 | 1 | 157 | 1 | 157 | 7.55e-68 | 203 |
| MsG0880047084.01.T01 | MTR_2g035190 | 64.331 | 157 | 56 | 0 | 1 | 157 | 1 | 157 | 1.62e-67 | 202 |
| MsG0880047084.01.T01 | MTR_2g035220 | 64.968 | 157 | 55 | 0 | 1 | 157 | 1 | 157 | 1.33e-66 | 200 |
| MsG0880047084.01.T01 | MTR_2g035170 | 55.414 | 157 | 70 | 0 | 1 | 157 | 1 | 157 | 2.47e-56 | 174 |
| MsG0880047084.01.T01 | MTR_4g120940 | 97.297 | 74 | 2 | 0 | 85 | 158 | 47 | 120 | 1.84e-44 | 142 |
| MsG0880047084.01.T01 | MTR_2g035320 | 59.596 | 99 | 40 | 0 | 59 | 157 | 24 | 122 | 4.91e-37 | 124 |
| MsG0880047084.01.T01 | MTR_4g120950 | 45.342 | 161 | 83 | 4 | 1 | 158 | 1 | 159 | 3.38e-36 | 123 |
| MsG0880047084.01.T01 | MTR_2g435310 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 7.82e-34 | 117 |
| MsG0880047084.01.T01 | MTR_4g120760 | 37.580 | 157 | 97 | 1 | 1 | 156 | 1 | 157 | 1.17e-33 | 116 |
| MsG0880047084.01.T01 | MTR_4g120760 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 1.37e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 47 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATCAATTGCATCAATT+TGG | 0.214492 | 8:+81701678 | None:intergenic |
| TTGATGGCATCAACAGCTTT+TGG | 0.227157 | 8:+81702044 | None:intergenic |
| GATATCATTTGAAGCGAAAT+TGG | 0.228714 | 8:-81701594 | MsG0880047084.01.T01:CDS |
| AGGATGGATTAGTTGTAATT+AGG | 0.259719 | 8:+81701422 | None:intergenic |
| GGCCATTGAGGGTTATGTTT+TGG | 0.293726 | 8:-81701450 | MsG0880047084.01.T01:CDS |
| GGTCCAAATGGAGGGTCTAT+TGG | 0.333504 | 8:-81701566 | MsG0880047084.01.T01:CDS |
| TCCTTTGGTTTGATATTTAA+CGG | 0.347214 | 8:+81701535 | None:intergenic |
| GACGGTTTCAACACTCTTGA+TGG | 0.351518 | 8:+81702028 | None:intergenic |
| GTCCAAATGGAGGGTCTATT+GGG | 0.390136 | 8:-81701565 | MsG0880047084.01.T01:CDS |
| AATTGGCTTAGCATCTCCTT+TGG | 0.398977 | 8:+81701520 | None:intergenic |
| TTCCCAATAGACCCTCCATT+TGG | 0.416216 | 8:+81701563 | None:intergenic |
| TGCAATTGATGAAGCAAACT+TGG | 0.424818 | 8:-81701669 | MsG0880047084.01.T01:CDS |
| AGAGCTTTGTAAAGCTTAGC+AGG | 0.435032 | 8:+81702092 | None:intergenic |
| AACGAAAGTGAGCTTCTTGA+CGG | 0.435771 | 8:+81701983 | None:intergenic |
| GTTGAAACCGTCGAGGGAAA+TGG | 0.443139 | 8:-81702017 | MsG0880047084.01.T01:CDS |
| AAGAAGCTCACTTTCGTTGA+GGG | 0.454166 | 8:-81701978 | MsG0880047084.01.T01:intron |
| GGATTAGTTGTAATTAGGAT+TGG | 0.464473 | 8:+81701427 | None:intergenic |
| TATAGTATAGTTGGAGGAGT+CGG | 0.473938 | 8:-81701638 | MsG0880047084.01.T01:CDS |
| GGTTGAGGAAGGCAAGGCAA+AGG | 0.481278 | 8:-81701489 | MsG0880047084.01.T01:CDS |
| TTTGAAGCGAAATTGGTTGA+AGG | 0.487809 | 8:-81701587 | MsG0880047084.01.T01:CDS |
| GGGTATAACTATAGTATAGT+TGG | 0.489142 | 8:-81701647 | MsG0880047084.01.T01:CDS |
| AATTTGGTGCAGCACGTACA+AGG | 0.503363 | 8:+81701694 | None:intergenic |
| ATAGTATAGTTGGAGGAGTC+GGG | 0.510010 | 8:-81701637 | MsG0880047084.01.T01:CDS |
| AGATGCTAAGCCAATTGAAA+AGG | 0.515464 | 8:-81701513 | MsG0880047084.01.T01:CDS |
| GCCAATTGAAAAGGAGGTTG+AGG | 0.520188 | 8:-81701504 | MsG0880047084.01.T01:CDS |
| GGCCAAAACATAACCCTCAA+TGG | 0.521409 | 8:+81701448 | None:intergenic |
| GTCGAGGGAAATGGTGGTGC+CGG | 0.524142 | 8:-81702008 | MsG0880047084.01.T01:CDS |
| GGCACCACCATTTCCCTCGA+CGG | 0.527211 | 8:+81702010 | None:intergenic |
| AGTGAGCTTCTTGACGGTGC+CGG | 0.531057 | 8:+81701989 | None:intergenic |
| CAAGAAGCTCACTTTCGTTG+AGG | 0.534631 | 8:-81701979 | MsG0880047084.01.T01:CDS |
| GTTGAGGAAGGCAAGGCAAA+GGG | 0.544771 | 8:-81701488 | MsG0880047084.01.T01:CDS |
| AAATTGGTTGAAGGTCCAAA+TGG | 0.568255 | 8:-81701578 | MsG0880047084.01.T01:CDS |
| GCAATTGATGAAGCAAACTT+GGG | 0.569895 | 8:-81701668 | MsG0880047084.01.T01:CDS |
| AGGAGTCGGGTTGCCAGACA+CGG | 0.576437 | 8:-81701624 | MsG0880047084.01.T01:CDS |
| TATAACTATAGTATAGTTGG+AGG | 0.584231 | 8:-81701644 | MsG0880047084.01.T01:CDS |
| AGCAGGAGCAACTGTAGAGG+TGG | 0.585421 | 8:+81702109 | None:intergenic |
| CTTAGCAGGAGCAACTGTAG+AGG | 0.591469 | 8:+81702106 | None:intergenic |
| TGCTAAGCCAATTGAAAAGG+AGG | 0.591628 | 8:-81701510 | MsG0880047084.01.T01:CDS |
| ACCGTTAAATATCAAACCAA+AGG | 0.596206 | 8:-81701536 | MsG0880047084.01.T01:CDS |
| TGGTTGAAGGTCCAAATGGA+GGG | 0.601542 | 8:-81701574 | MsG0880047084.01.T01:CDS |
| TTGGTTGAAGGTCCAAATGG+AGG | 0.609517 | 8:-81701575 | MsG0880047084.01.T01:CDS |
| ATTGAAAAGGAGGTTGAGGA+AGG | 0.616285 | 8:-81701500 | MsG0880047084.01.T01:CDS |
| AAAGGAGGTTGAGGAAGGCA+AGG | 0.629508 | 8:-81701495 | MsG0880047084.01.T01:CDS |
| AAGAGTGTTGAAACCGTCGA+GGG | 0.665491 | 8:-81702023 | MsG0880047084.01.T01:CDS |
| CAAGAGTGTTGAAACCGTCG+AGG | 0.677120 | 8:-81702024 | MsG0880047084.01.T01:CDS |
| GAAACCGTCGAGGGAAATGG+TGG | 0.692349 | 8:-81702014 | MsG0880047084.01.T01:CDS |
| CAATTGATGAAGCAAACTTG+GGG | 0.696928 | 8:-81701667 | MsG0880047084.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTCATCTTTACGTAAAAA+AGG | - | Chr8:81701689-81701708 | MsG0880047084.01.T01:CDS | 25.0% |
| ! | ATAATATGTTGATGCATCAA+AGG | + | Chr8:81701735-81701754 | None:intergenic | 25.0% |
| ! | TATAACTATAGTATAGTTGG+AGG | - | Chr8:81701923-81701942 | MsG0880047084.01.T01:intron | 25.0% |
| !!! | TCCTTTGGTTTGATATTTAA+CGG | + | Chr8:81702035-81702054 | None:intergenic | 25.0% |
| AAAGATGAGTTAAGAGTGAT+TGG | + | Chr8:81701680-81701699 | None:intergenic | 30.0% | |
| AACATAAATATGTATGCAGG+AGG | - | Chr8:81701845-81701864 | MsG0880047084.01.T01:intron | 30.0% | |
| ACATAAATATGTATGCAGGA+GGG | - | Chr8:81701846-81701865 | MsG0880047084.01.T01:intron | 30.0% | |
| ACCAACATAAATATGTATGC+AGG | - | Chr8:81701842-81701861 | MsG0880047084.01.T01:intron | 30.0% | |
| ACCGTTAAATATCAAACCAA+AGG | - | Chr8:81702031-81702050 | MsG0880047084.01.T01:CDS | 30.0% | |
| CTTCATCAATTGCATCAATT+TGG | + | Chr8:81701892-81701911 | None:intergenic | 30.0% | |
| GGGTATAACTATAGTATAGT+TGG | - | Chr8:81701920-81701939 | MsG0880047084.01.T01:intron | 30.0% | |
| TCCTGCATACATATTTATGT+TGG | + | Chr8:81701846-81701865 | None:intergenic | 30.0% | |
| ! | GATATCATTTGAAGCGAAAT+TGG | - | Chr8:81701973-81701992 | MsG0880047084.01.T01:intron | 30.0% |
| !!! | ATTGGAGAAGTTTTAAACAC+GGG | + | Chr8:81701662-81701681 | None:intergenic | 30.0% |
| !!! | GATTGGAGAAGTTTTAAACA+CGG | + | Chr8:81701663-81701682 | None:intergenic | 30.0% |
| AAATTGGTTGAAGGTCCAAA+TGG | - | Chr8:81701989-81702008 | MsG0880047084.01.T01:CDS | 35.0% | |
| AGATGCTAAGCCAATTGAAA+AGG | - | Chr8:81702054-81702073 | MsG0880047084.01.T01:CDS | 35.0% | |
| CAATTGATGAAGCAAACTTG+GGG | - | Chr8:81701900-81701919 | MsG0880047084.01.T01:intron | 35.0% | |
| GCAATTGATGAAGCAAACTT+GGG | - | Chr8:81701899-81701918 | MsG0880047084.01.T01:intron | 35.0% | |
| TATAGTATAGTTGGAGGAGT+CGG | - | Chr8:81701929-81701948 | MsG0880047084.01.T01:intron | 35.0% | |
| TGCAATTGATGAAGCAAACT+TGG | - | Chr8:81701898-81701917 | MsG0880047084.01.T01:intron | 35.0% | |
| ! | TTTGAAGCGAAATTGGTTGA+AGG | - | Chr8:81701980-81701999 | MsG0880047084.01.T01:CDS | 35.0% |
| AACGAAAGTGAGCTTCTTGA+CGG | + | Chr8:81701587-81701606 | None:intergenic | 40.0% | |
| AAGAAGCTCACTTTCGTTGA+GGG | - | Chr8:81701589-81701608 | MsG0880047084.01.T01:CDS | 40.0% | |
| ATAGTATAGTTGGAGGAGTC+GGG | - | Chr8:81701930-81701949 | MsG0880047084.01.T01:intron | 40.0% | |
| ATTGAAAAGGAGGTTGAGGA+AGG | - | Chr8:81702067-81702086 | MsG0880047084.01.T01:CDS | 40.0% | |
| TGCTAAGCCAATTGAAAAGG+AGG | - | Chr8:81702057-81702076 | MsG0880047084.01.T01:CDS | 40.0% | |
| ! | AAAGGGTGATGCTCTTTTCA+AGG | - | Chr8:81702096-81702115 | MsG0880047084.01.T01:CDS | 40.0% |
| ! | AATTGGCTTAGCATCTCCTT+TGG | + | Chr8:81702050-81702069 | None:intergenic | 40.0% |
| ! | AGAGCTTTGTAAAGCTTAGC+AGG | + | Chr8:81701478-81701497 | None:intergenic | 40.0% |
| ! | GATATCTTTTCAACCGTGTC+TGG | + | Chr8:81701959-81701978 | None:intergenic | 40.0% |
| ! | TCCTCAACCTCCTTTTCAAT+TGG | + | Chr8:81702067-81702086 | None:intergenic | 40.0% |
| ! | TGATGGCATCAACAGCTTTT+GGG | + | Chr8:81701525-81701544 | None:intergenic | 40.0% |
| !! | TTGATGGCATCAACAGCTTT+TGG | + | Chr8:81701526-81701545 | None:intergenic | 40.0% |
| CAAGAAGCTCACTTTCGTTG+AGG | - | Chr8:81701588-81701607 | MsG0880047084.01.T01:CDS | 45.0% | |
| GACGGTTTCAACACTCTTGA+TGG | + | Chr8:81701542-81701561 | None:intergenic | 45.0% | |
| GCCAATTGAAAAGGAGGTTG+AGG | - | Chr8:81702063-81702082 | MsG0880047084.01.T01:CDS | 45.0% | |
| GGCCAAAACATAACCCTCAA+TGG | + | Chr8:81702122-81702141 | None:intergenic | 45.0% | |
| GTCCAAATGGAGGGTCTATT+GGG | - | Chr8:81702002-81702021 | MsG0880047084.01.T01:CDS | 45.0% | |
| TGGTTGAAGGTCCAAATGGA+GGG | - | Chr8:81701993-81702012 | MsG0880047084.01.T01:CDS | 45.0% | |
| TTCCCAATAGACCCTCCATT+TGG | + | Chr8:81702007-81702026 | None:intergenic | 45.0% | |
| TTGGTTGAAGGTCCAAATGG+AGG | - | Chr8:81701992-81702011 | MsG0880047084.01.T01:CDS | 45.0% | |
| ! | GGCCATTGAGGGTTATGTTT+TGG | - | Chr8:81702117-81702136 | MsG0880047084.01.T01:CDS | 45.0% |
| !! | AAGAGTGTTGAAACCGTCGA+GGG | - | Chr8:81701544-81701563 | MsG0880047084.01.T01:CDS | 45.0% |
| !! | AATTTGGTGCAGCACGTACA+AGG | + | Chr8:81701876-81701895 | None:intergenic | 45.0% |
| !! | GCTCTTTTCAAGGCCATTGA+GGG | - | Chr8:81702106-81702125 | MsG0880047084.01.T01:CDS | 45.0% |
| !! | TGCTCTTTTCAAGGCCATTG+AGG | - | Chr8:81702105-81702124 | MsG0880047084.01.T01:CDS | 45.0% |
| AAAGGAGGTTGAGGAAGGCA+AGG | - | Chr8:81702072-81702091 | MsG0880047084.01.T01:CDS | 50.0% | |
| CTTAGCAGGAGCAACTGTAG+AGG | + | Chr8:81701464-81701483 | None:intergenic | 50.0% | |
| GGTCCAAATGGAGGGTCTAT+TGG | - | Chr8:81702001-81702020 | MsG0880047084.01.T01:CDS | 50.0% | |
| GTTGAAACCGTCGAGGGAAA+TGG | - | Chr8:81701550-81701569 | MsG0880047084.01.T01:CDS | 50.0% | |
| GTTGAGGAAGGCAAGGCAAA+GGG | - | Chr8:81702079-81702098 | MsG0880047084.01.T01:CDS | 50.0% | |
| !! | CAAGAGTGTTGAAACCGTCG+AGG | - | Chr8:81701543-81701562 | MsG0880047084.01.T01:CDS | 50.0% |
| AGCAGGAGCAACTGTAGAGG+TGG | + | Chr8:81701461-81701480 | None:intergenic | 55.0% | |
| AGTGAGCTTCTTGACGGTGC+CGG | + | Chr8:81701581-81701600 | None:intergenic | 55.0% | |
| GAAACCGTCGAGGGAAATGG+TGG | - | Chr8:81701553-81701572 | MsG0880047084.01.T01:CDS | 55.0% | |
| GGTTGAGGAAGGCAAGGCAA+AGG | - | Chr8:81702078-81702097 | MsG0880047084.01.T01:CDS | 55.0% | |
| GGCACCACCATTTCCCTCGA+CGG | + | Chr8:81701560-81701579 | None:intergenic | 60.0% | |
| ! | AGGAGTCGGGTTGCCAGACA+CGG | - | Chr8:81701943-81701962 | MsG0880047084.01.T01:intron | 60.0% |
| ! | GTCGAGGGAAATGGTGGTGC+CGG | - | Chr8:81701559-81701578 | MsG0880047084.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 81701430 | 81702159 | 81701430 | ID=MsG0880047084.01;Name=MsG0880047084.01 |
| Chr8 | mRNA | 81701430 | 81702159 | 81701430 | ID=MsG0880047084.01.T01;Parent=MsG0880047084.01;Name=MsG0880047084.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|0.5|2|0|158 |
| Chr8 | exon | 81701979 | 81702159 | 81701979 | ID=MsG0880047084.01.T01:exon:35165;Parent=MsG0880047084.01.T01 |
| Chr8 | exon | 81701430 | 81701725 | 81701430 | ID=MsG0880047084.01.T01:exon:35164;Parent=MsG0880047084.01.T01 |
| Chr8 | CDS | 81701979 | 81702159 | 81701979 | ID=MsG0880047084.01.T01:cds;Parent=MsG0880047084.01.T01 |
| Chr8 | CDS | 81701430 | 81701725 | 81701430 | ID=MsG0880047084.01.T01:cds;Parent=MsG0880047084.01.T01 |
| Gene Sequence |
| Protein sequence |
