Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047085.01.T01 | XP_013458291.3 | 97.468 | 158 | 4 | 0 | 1 | 158 | 1 | 158 | 6.42E-105 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047085.01.T01 | Q06930 | 80.38 | 158 | 31 | 0 | 1 | 158 | 1 | 158 | 1.98E-89 | 260 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047085.01.T01 | A0A396IFH7 | 97.468 | 158 | 4 | 0 | 1 | 158 | 1 | 158 | 3.06e-105 | 306 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000148.01 | MsG0880047085.01 | 0.804949 | 1.690122e-49 | 8.830388e-47 |
| MsG0880044342.01 | MsG0880047085.01 | 0.819486 | 1.132529e-52 | 8.727164e-50 |
| MsG0880047084.01 | MsG0880047085.01 | 0.948605 | 6.774308e-107 | 1.276430e-101 |
| MsG0180005372.01 | MsG0880047085.01 | 0.804361 | 2.241930e-49 | 1.153650e-46 |
| MsG0880042591.01 | MsG0880047085.01 | 0.829156 | 6.026935e-55 | 6.117611e-52 |
| MsG0880043669.01 | MsG0880047085.01 | 0.828758 | 7.522788e-55 | 7.546705e-52 |
| MsG0280011165.01 | MsG0880047085.01 | 0.805124 | 1.552836e-49 | 8.149598e-47 |
| MsG0280011203.01 | MsG0880047085.01 | 0.809419 | 1.910063e-50 | 1.120935e-47 |
| MsG0480019171.01 | MsG0880047085.01 | 0.803220 | 3.868186e-49 | 1.932961e-46 |
| MsG0580026019.01 | MsG0880047085.01 | 0.822894 | 1.855835e-53 | 1.573573e-50 |
| MsG0680030296.01 | MsG0880047085.01 | 0.809464 | 1.868448e-50 | 1.097756e-47 |
| MsG0680030613.01 | MsG0880047085.01 | 0.822843 | 1.906542e-53 | 1.614312e-50 |
| MsG0280007911.01 | MsG0880047085.01 | 0.808905 | 2.461390e-50 | 1.424634e-47 |
| MsG0280009432.01 | MsG0880047085.01 | 0.804835 | 1.784666e-49 | 9.297215e-47 |
| MsG0780036661.01 | MsG0880047085.01 | 0.806619 | 7.534157e-50 | 4.108105e-47 |
| MsG0780036726.01 | MsG0880047085.01 | 0.841603 | 4.298161e-58 | 6.348631e-55 |
| MsG0780038305.01 | MsG0880047085.01 | 0.803902 | 2.792438e-49 | 1.420179e-46 |
| MsG0780039619.01 | MsG0880047085.01 | 0.807890 | 4.052741e-50 | 2.283930e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047085.01.T01 | MTR_6g033450 | 96.203 | 158 | 6 | 0 | 1 | 158 | 1 | 158 | 1.22e-107 | 303 |
| MsG0880047085.01.T01 | MTR_4g120970 | 96.203 | 158 | 6 | 0 | 1 | 158 | 1 | 158 | 1.22e-107 | 303 |
| MsG0880047085.01.T01 | MTR_2g035150 | 74.684 | 158 | 40 | 0 | 1 | 158 | 1 | 158 | 1.36e-82 | 240 |
| MsG0880047085.01.T01 | MTR_2g035100 | 73.248 | 157 | 42 | 0 | 1 | 157 | 1 | 157 | 4.28e-81 | 236 |
| MsG0880047085.01.T01 | MTR_2g035105 | 72.611 | 157 | 43 | 0 | 1 | 157 | 1 | 157 | 4.05e-80 | 234 |
| MsG0880047085.01.T01 | MTR_2g035130 | 70.701 | 157 | 46 | 0 | 1 | 157 | 1 | 157 | 7.39e-79 | 231 |
| MsG0880047085.01.T01 | MTR_2g035120 | 68.987 | 158 | 49 | 0 | 1 | 158 | 1 | 158 | 1.49e-76 | 225 |
| MsG0880047085.01.T01 | MTR_2g035320 | 64.331 | 157 | 56 | 0 | 1 | 157 | 1 | 157 | 2.25e-69 | 207 |
| MsG0880047085.01.T01 | MTR_2g035210 | 64.968 | 157 | 55 | 0 | 1 | 157 | 1 | 157 | 6.36e-69 | 206 |
| MsG0880047085.01.T01 | MTR_2g035190 | 64.331 | 157 | 56 | 0 | 1 | 157 | 1 | 157 | 3.18e-68 | 204 |
| MsG0880047085.01.T01 | MTR_2g035220 | 64.968 | 157 | 55 | 0 | 1 | 157 | 1 | 157 | 2.49e-67 | 202 |
| MsG0880047085.01.T01 | MTR_2g035170 | 55.414 | 157 | 70 | 0 | 1 | 157 | 1 | 157 | 3.66e-56 | 173 |
| MsG0880047085.01.T01 | MTR_4g120940 | 95.946 | 74 | 3 | 0 | 85 | 158 | 47 | 120 | 8.24e-44 | 141 |
| MsG0880047085.01.T01 | MTR_4g120950 | 45.963 | 161 | 82 | 4 | 1 | 158 | 1 | 159 | 4.68e-37 | 125 |
| MsG0880047085.01.T01 | MTR_2g035320 | 59.596 | 99 | 40 | 0 | 59 | 157 | 24 | 122 | 1.13e-36 | 123 |
| MsG0880047085.01.T01 | MTR_2g435310 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 1.19e-33 | 116 |
| MsG0880047085.01.T01 | MTR_4g120760 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 1.60e-33 | 116 |
| MsG0880047085.01.T01 | MTR_4g120760 | 37.580 | 157 | 97 | 1 | 1 | 156 | 1 | 157 | 1.69e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 50 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATCAATTGCATCAATT+TGG | 0.214492 | 8:+81707062 | None:intergenic |
| TTGATGGCATCAACAGCTTT+TGG | 0.227157 | 8:+81707318 | None:intergenic |
| GATATCATTTGAAGCGAAAT+TGG | 0.228714 | 8:-81706978 | MsG0880047085.01.T01:CDS |
| TAGATGGATTAGTTGTAATT+AGG | 0.259719 | 8:+81706806 | None:intergenic |
| GGCCATTGAGGGTTATGTTT+TGG | 0.293726 | 8:-81706834 | MsG0880047085.01.T01:CDS |
| AACGGTTTCAACACTCTTGA+TGG | 0.328088 | 8:+81707302 | None:intergenic |
| GGTCCAAATGGAGGGTCTAT+TGG | 0.333504 | 8:-81706950 | MsG0880047085.01.T01:CDS |
| TCCTTTGGTTTGATATTTAA+CGG | 0.347214 | 8:+81706919 | None:intergenic |
| GTCCAAATGGAGGGTCTATT+GGG | 0.390136 | 8:-81706949 | MsG0880047085.01.T01:CDS |
| AATTGGCTTAGCATCTCCTT+TGG | 0.398977 | 8:+81706904 | None:intergenic |
| GTGAGCTTCTTGACGGTGCC+AGG | 0.415394 | 8:+81707264 | None:intergenic |
| TTCCCAATAGACCCTCCATT+TGG | 0.416216 | 8:+81706947 | None:intergenic |
| TGCAATTGATGAAGCAAACT+TGG | 0.424818 | 8:-81707053 | MsG0880047085.01.T01:CDS |
| GCCAATTGAAAAGGAGGTTA+AGG | 0.429445 | 8:-81706888 | MsG0880047085.01.T01:CDS |
| AGAGCTTTGTAAAGCTTAGC+AGG | 0.435032 | 8:+81707366 | None:intergenic |
| AACGAAAGTGAGCTTCTTGA+CGG | 0.435771 | 8:+81707257 | None:intergenic |
| AAGAAGCTCACTTTCGTTGA+GGG | 0.454166 | 8:-81707252 | MsG0880047085.01.T01:intron |
| GGATTAGTTGTAATTAGGAT+TGG | 0.464473 | 8:+81706811 | None:intergenic |
| TATAGTATAGTTGGAGGAGT+CGG | 0.473938 | 8:-81707022 | MsG0880047085.01.T01:CDS |
| AGCAGGAGCAACTGTGGAGG+TGG | 0.481846 | 8:+81707383 | None:intergenic |
| GTTGAAACCGTTGAGGGAAA+TGG | 0.483004 | 8:-81707291 | MsG0880047085.01.T01:CDS |
| TTTGAAGCGAAATTGGTTGA+AGG | 0.487809 | 8:-81706971 | MsG0880047085.01.T01:CDS |
| GGGTATAACTATAGTATAGT+TGG | 0.489142 | 8:-81707031 | MsG0880047085.01.T01:CDS |
| GGTTAAGGAAGGCAAGGCAA+AGG | 0.502513 | 8:-81706873 | MsG0880047085.01.T01:CDS |
| AATTTGGTGCAGCACGTACA+AGG | 0.503363 | 8:+81707078 | None:intergenic |
| ATAGTATAGTTGGAGGAGTC+GGG | 0.510010 | 8:-81707021 | MsG0880047085.01.T01:CDS |
| GTTGAGGGAAATGGTGGCCC+TGG | 0.512110 | 8:-81707282 | MsG0880047085.01.T01:CDS |
| AGATGCTAAGCCAATTGAAA+AGG | 0.515464 | 8:-81706897 | MsG0880047085.01.T01:CDS |
| GGCCAAAACATAACCCTCAA+TGG | 0.521409 | 8:+81706832 | None:intergenic |
| AGGGCCACCATTTCCCTCAA+CGG | 0.521721 | 8:+81707284 | None:intergenic |
| CAAGAAGCTCACTTTCGTTG+AGG | 0.534631 | 8:-81707253 | MsG0880047085.01.T01:CDS |
| AAGAGTGTTGAAACCGTTGA+GGG | 0.546792 | 8:-81707297 | MsG0880047085.01.T01:CDS |
| GTTAAGGAAGGCAAGGCAAA+GGG | 0.562140 | 8:-81706872 | MsG0880047085.01.T01:CDS |
| GGATGATATCATCAGAGTCA+TGG | 0.567722 | 8:+81707340 | None:intergenic |
| AAATTGGTTGAAGGTCCAAA+TGG | 0.568255 | 8:-81706962 | MsG0880047085.01.T01:CDS |
| GCAATTGATGAAGCAAACTT+GGG | 0.569895 | 8:-81707052 | MsG0880047085.01.T01:CDS |
| AGGAGTCGGGTTGCCAGACA+CGG | 0.576437 | 8:-81707008 | MsG0880047085.01.T01:CDS |
| TGCTAAGCCAATTGAAAAGG+AGG | 0.576674 | 8:-81706894 | MsG0880047085.01.T01:CDS |
| TATAACTATAGTATAGTTGG+AGG | 0.584231 | 8:-81707028 | MsG0880047085.01.T01:CDS |
| ACCGTTAAATATCAAACCAA+AGG | 0.596206 | 8:-81706920 | MsG0880047085.01.T01:CDS |
| TGGTTGAAGGTCCAAATGGA+GGG | 0.601542 | 8:-81706958 | MsG0880047085.01.T01:CDS |
| TGAGCTTCTTGACGGTGCCA+GGG | 0.607223 | 8:+81707265 | None:intergenic |
| TTGGTTGAAGGTCCAAATGG+AGG | 0.609517 | 8:-81706959 | MsG0880047085.01.T01:CDS |
| AAGCTTAGCAGGAGCAACTG+TGG | 0.620370 | 8:+81707377 | None:intergenic |
| CAAGAGTGTTGAAACCGTTG+AGG | 0.622991 | 8:-81707298 | MsG0880047085.01.T01:CDS |
| ATTGAAAAGGAGGTTAAGGA+AGG | 0.629324 | 8:-81706884 | MsG0880047085.01.T01:CDS |
| AAAGGAGGTTAAGGAAGGCA+AGG | 0.634245 | 8:-81706879 | MsG0880047085.01.T01:CDS |
| CTTAGCAGGAGCAACTGTGG+AGG | 0.671364 | 8:+81707380 | None:intergenic |
| CAATTGATGAAGCAAACTTG+GGG | 0.696928 | 8:-81707051 | MsG0880047085.01.T01:CDS |
| GAAACCGTTGAGGGAAATGG+TGG | 0.702528 | 8:-81707288 | MsG0880047085.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATAACTATAGTATAGTTGG+AGG | - | Chr8:81707197-81707216 | MsG0880047085.01.T01:intron | 25.0% |
| !!! | TCCTTTGGTTTGATATTTAA+CGG | + | Chr8:81707309-81707328 | None:intergenic | 25.0% |
| ACCGTTAAATATCAAACCAA+AGG | - | Chr8:81707305-81707324 | MsG0880047085.01.T01:CDS | 30.0% | |
| CTTCATCAATTGCATCAATT+TGG | + | Chr8:81707166-81707185 | None:intergenic | 30.0% | |
| GGGTATAACTATAGTATAGT+TGG | - | Chr8:81707194-81707213 | MsG0880047085.01.T01:intron | 30.0% | |
| ! | GATATCATTTGAAGCGAAAT+TGG | - | Chr8:81707247-81707266 | MsG0880047085.01.T01:intron | 30.0% |
| !! | TGTCGCACATGTTTTTAATA+TGG | - | Chr8:81707055-81707074 | MsG0880047085.01.T01:CDS | 30.0% |
| AAATTGGTTGAAGGTCCAAA+TGG | - | Chr8:81707263-81707282 | MsG0880047085.01.T01:CDS | 35.0% | |
| AACATAAATATGTACGCAGG+AGG | - | Chr8:81707119-81707138 | MsG0880047085.01.T01:intron | 35.0% | |
| ACATAAATATGTACGCAGGA+GGG | - | Chr8:81707120-81707139 | MsG0880047085.01.T01:intron | 35.0% | |
| ACCAACATAAATATGTACGC+AGG | - | Chr8:81707116-81707135 | MsG0880047085.01.T01:intron | 35.0% | |
| AGATGCTAAGCCAATTGAAA+AGG | - | Chr8:81707328-81707347 | MsG0880047085.01.T01:CDS | 35.0% | |
| ATTGAAAAGGAGGTTAAGGA+AGG | - | Chr8:81707341-81707360 | MsG0880047085.01.T01:CDS | 35.0% | |
| CAATTGATGAAGCAAACTTG+GGG | - | Chr8:81707174-81707193 | MsG0880047085.01.T01:intron | 35.0% | |
| GCAATTGATGAAGCAAACTT+GGG | - | Chr8:81707173-81707192 | MsG0880047085.01.T01:intron | 35.0% | |
| TATAGTATAGTTGGAGGAGT+CGG | - | Chr8:81707203-81707222 | MsG0880047085.01.T01:intron | 35.0% | |
| TCCTGCGTACATATTTATGT+TGG | + | Chr8:81707120-81707139 | None:intergenic | 35.0% | |
| TGCAATTGATGAAGCAAACT+TGG | - | Chr8:81707172-81707191 | MsG0880047085.01.T01:intron | 35.0% | |
| ! | TCCTTAACCTCCTTTTCAAT+TGG | + | Chr8:81707341-81707360 | None:intergenic | 35.0% |
| ! | TTTGAAGCGAAATTGGTTGA+AGG | - | Chr8:81707254-81707273 | MsG0880047085.01.T01:CDS | 35.0% |
| AACGAAAGTGAGCTTCTTGA+CGG | + | Chr8:81706971-81706990 | None:intergenic | 40.0% | |
| AACGGTTTCAACACTCTTGA+TGG | + | Chr8:81706926-81706945 | None:intergenic | 40.0% | |
| AAGAAGCTCACTTTCGTTGA+GGG | - | Chr8:81706973-81706992 | MsG0880047085.01.T01:CDS | 40.0% | |
| ATAGTATAGTTGGAGGAGTC+GGG | - | Chr8:81707204-81707223 | MsG0880047085.01.T01:intron | 40.0% | |
| GCCAATTGAAAAGGAGGTTA+AGG | - | Chr8:81707337-81707356 | MsG0880047085.01.T01:CDS | 40.0% | |
| GGATGATATCATCAGAGTCA+TGG | + | Chr8:81706888-81706907 | None:intergenic | 40.0% | |
| TGCTAAGCCAATTGAAAAGG+AGG | - | Chr8:81707331-81707350 | MsG0880047085.01.T01:CDS | 40.0% | |
| ! | AAAGGGTGATGCTCTTTTCA+AGG | - | Chr8:81707370-81707389 | MsG0880047085.01.T01:CDS | 40.0% |
| ! | AATTGGCTTAGCATCTCCTT+TGG | + | Chr8:81707324-81707343 | None:intergenic | 40.0% |
| ! | AGAGCTTTGTAAAGCTTAGC+AGG | + | Chr8:81706862-81706881 | None:intergenic | 40.0% |
| ! | GATATCTTTTCAACCGTGTC+TGG | + | Chr8:81707233-81707252 | None:intergenic | 40.0% |
| ! | TGATGGCATCAACAGCTTTT+GGG | + | Chr8:81706909-81706928 | None:intergenic | 40.0% |
| !! | AAGAGTGTTGAAACCGTTGA+GGG | - | Chr8:81706928-81706947 | MsG0880047085.01.T01:CDS | 40.0% |
| !! | TTGATGGCATCAACAGCTTT+TGG | + | Chr8:81706910-81706929 | None:intergenic | 40.0% |
| AAAGGAGGTTAAGGAAGGCA+AGG | - | Chr8:81707346-81707365 | MsG0880047085.01.T01:CDS | 45.0% | |
| CAAGAAGCTCACTTTCGTTG+AGG | - | Chr8:81706972-81706991 | MsG0880047085.01.T01:CDS | 45.0% | |
| GGCCAAAACATAACCCTCAA+TGG | + | Chr8:81707396-81707415 | None:intergenic | 45.0% | |
| GTCCAAATGGAGGGTCTATT+GGG | - | Chr8:81707276-81707295 | MsG0880047085.01.T01:CDS | 45.0% | |
| GTTAAGGAAGGCAAGGCAAA+GGG | - | Chr8:81707353-81707372 | MsG0880047085.01.T01:CDS | 45.0% | |
| GTTGAAACCGTTGAGGGAAA+TGG | - | Chr8:81706934-81706953 | MsG0880047085.01.T01:CDS | 45.0% | |
| TGGTTGAAGGTCCAAATGGA+GGG | - | Chr8:81707267-81707286 | MsG0880047085.01.T01:CDS | 45.0% | |
| TTCCCAATAGACCCTCCATT+TGG | + | Chr8:81707281-81707300 | None:intergenic | 45.0% | |
| TTGGTTGAAGGTCCAAATGG+AGG | - | Chr8:81707266-81707285 | MsG0880047085.01.T01:CDS | 45.0% | |
| ! | GGCCATTGAGGGTTATGTTT+TGG | - | Chr8:81707391-81707410 | MsG0880047085.01.T01:CDS | 45.0% |
| !! | AATTTGGTGCAGCACGTACA+AGG | + | Chr8:81707150-81707169 | None:intergenic | 45.0% |
| !! | CAAGAGTGTTGAAACCGTTG+AGG | - | Chr8:81706927-81706946 | MsG0880047085.01.T01:CDS | 45.0% |
| !! | GCTCTTTTCAAGGCCATTGA+GGG | - | Chr8:81707380-81707399 | MsG0880047085.01.T01:CDS | 45.0% |
| !! | TGCTCTTTTCAAGGCCATTG+AGG | - | Chr8:81707379-81707398 | MsG0880047085.01.T01:CDS | 45.0% |
| AAGCTTAGCAGGAGCAACTG+TGG | + | Chr8:81706851-81706870 | None:intergenic | 50.0% | |
| GAAACCGTTGAGGGAAATGG+TGG | - | Chr8:81706937-81706956 | MsG0880047085.01.T01:CDS | 50.0% | |
| GGTCCAAATGGAGGGTCTAT+TGG | - | Chr8:81707275-81707294 | MsG0880047085.01.T01:CDS | 50.0% | |
| GGTTAAGGAAGGCAAGGCAA+AGG | - | Chr8:81707352-81707371 | MsG0880047085.01.T01:CDS | 50.0% | |
| AGGGCCACCATTTCCCTCAA+CGG | + | Chr8:81706944-81706963 | None:intergenic | 55.0% | |
| CTTAGCAGGAGCAACTGTGG+AGG | + | Chr8:81706848-81706867 | None:intergenic | 55.0% | |
| ! | TGAGCTTCTTGACGGTGCCA+GGG | + | Chr8:81706963-81706982 | None:intergenic | 55.0% |
| AGCAGGAGCAACTGTGGAGG+TGG | + | Chr8:81706845-81706864 | None:intergenic | 60.0% | |
| GTGAGCTTCTTGACGGTGCC+AGG | + | Chr8:81706964-81706983 | None:intergenic | 60.0% | |
| GTTGAGGGAAATGGTGGCCC+TGG | - | Chr8:81706943-81706962 | MsG0880047085.01.T01:CDS | 60.0% | |
| ! | AGGAGTCGGGTTGCCAGACA+CGG | - | Chr8:81707217-81707236 | MsG0880047085.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 81706814 | 81707433 | 81706814 | ID=MsG0880047085.01;Name=MsG0880047085.01 |
| Chr8 | mRNA | 81706814 | 81707433 | 81706814 | ID=MsG0880047085.01.T01;Parent=MsG0880047085.01;Name=MsG0880047085.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|158 |
| Chr8 | exon | 81707253 | 81707433 | 81707253 | ID=MsG0880047085.01.T01:exon:35167;Parent=MsG0880047085.01.T01 |
| Chr8 | exon | 81706814 | 81707109 | 81706814 | ID=MsG0880047085.01.T01:exon:35166;Parent=MsG0880047085.01.T01 |
| Chr8 | CDS | 81707253 | 81707433 | 81707253 | ID=MsG0880047085.01.T01:cds;Parent=MsG0880047085.01.T01 |
| Chr8 | CDS | 81706814 | 81707109 | 81706814 | ID=MsG0880047085.01.T01:cds;Parent=MsG0880047085.01.T01 |
| Gene Sequence |
| Protein sequence |
