Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047611.01.T01 | XP_003610365.1 | 99.127 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 1.55E-164 | 463 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047611.01.T01 | Q9C8T1 | 83.406 | 229 | 38 | 0 | 1 | 229 | 1 | 229 | 5.28E-139 | 391 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047611.01.T01 | G7JHX5 | 99.127 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 7.39e-165 | 463 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0880045705.01 | MsG0880047611.01 | 0.804539 | 2.058333e-49 | 1.063999e-46 |
| MsG0180005962.01 | MsG0880047611.01 | 0.800739 | 1.251405e-48 | 5.871500e-46 |
| MsG0680031628.01 | MsG0880047611.01 | -0.805939 | 1.047740e-49 | 5.615058e-47 |
| MsG0480021448.01 | MsG0880047611.01 | -0.802502 | 5.442174e-49 | 2.670145e-46 |
| MsG0480022151.01 | MsG0880047611.01 | 0.804855 | 1.768171e-49 | 9.215867e-47 |
| MsG0380015598.01 | MsG0880047611.01 | 0.810376 | 1.188630e-50 | 7.152977e-48 |
| MsG0380016342.01 | MsG0880047611.01 | 0.829433 | 5.161978e-55 | 5.282388e-52 |
| MsG0780036544.01 | MsG0880047611.01 | 0.800125 | 1.668559e-48 | 7.707894e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0480022104.01 | MsG0880047611.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047611.01.T01 | MTR_4g131330 | 99.127 | 229 | 2 | 0 | 1 | 229 | 1 | 229 | 1.87e-168 | 463 |
| MsG0880047611.01.T01 | MTR_1567s0010 | 33.143 | 175 | 108 | 4 | 28 | 196 | 245 | 416 | 3.20e-19 | 85.9 |
| MsG0880047611.01.T01 | MTR_4g124050 | 29.592 | 196 | 111 | 4 | 20 | 197 | 1062 | 1248 | 6.17e-17 | 79.7 |
| MsG0880047611.01.T01 | MTR_4g124050 | 26.455 | 189 | 115 | 2 | 25 | 197 | 413 | 593 | 2.99e-12 | 65.9 |
| MsG0880047611.01.T01 | MTR_4g077930 | 30.233 | 172 | 95 | 2 | 24 | 178 | 941 | 1104 | 1.84e-15 | 75.5 |
| MsG0880047611.01.T01 | MTR_4g077930 | 30.233 | 172 | 95 | 2 | 24 | 178 | 1050 | 1213 | 2.03e-15 | 75.5 |
| MsG0880047611.01.T01 | MTR_3g107800 | 28.205 | 195 | 115 | 2 | 20 | 197 | 1038 | 1224 | 1.68e-14 | 72.8 |
| MsG0880047611.01.T01 | MTR_3g107800 | 27.835 | 194 | 116 | 3 | 20 | 197 | 408 | 593 | 1.29e-13 | 70.1 |
| MsG0880047611.01.T01 | MTR_1g086095 | 28.488 | 172 | 98 | 2 | 24 | 178 | 272 | 435 | 2.99e-14 | 71.6 |
| MsG0880047611.01.T01 | MTR_4g124000 | 27.907 | 172 | 99 | 2 | 24 | 178 | 1052 | 1215 | 6.19e-14 | 70.9 |
| MsG0880047611.01.T01 | MTR_4g124000 | 29.412 | 170 | 96 | 3 | 25 | 178 | 395 | 556 | 3.07e-13 | 68.9 |
| MsG0880047611.01.T01 | MTR_1g086150 | 28.488 | 172 | 98 | 2 | 24 | 178 | 1055 | 1218 | 6.67e-14 | 70.9 |
| MsG0880047611.01.T01 | MTR_6g465300 | 29.082 | 196 | 112 | 6 | 19 | 196 | 482 | 668 | 6.80e-14 | 70.9 |
| MsG0880047611.01.T01 | MTR_6g465300 | 29.082 | 196 | 112 | 6 | 19 | 196 | 482 | 668 | 7.42e-14 | 70.5 |
| MsG0880047611.01.T01 | MTR_1g086080 | 28.488 | 172 | 98 | 2 | 24 | 178 | 1060 | 1223 | 7.77e-14 | 70.9 |
| MsG0880047611.01.T01 | MTR_1g086080 | 27.647 | 170 | 99 | 3 | 25 | 178 | 406 | 567 | 7.15e-11 | 62.0 |
| MsG0880047611.01.T01 | MTR_4g124000 | 28.042 | 189 | 112 | 3 | 25 | 197 | 395 | 575 | 1.67e-13 | 69.7 |
| MsG0880047611.01.T01 | MTR_4g123990 | 29.412 | 170 | 96 | 3 | 25 | 178 | 395 | 556 | 2.93e-13 | 68.9 |
| MsG0880047611.01.T01 | MTR_4g123990 | 26.744 | 172 | 101 | 2 | 24 | 178 | 1052 | 1215 | 6.83e-13 | 67.8 |
| MsG0880047611.01.T01 | MTR_1g063170 | 28.655 | 171 | 98 | 2 | 24 | 178 | 415 | 577 | 3.00e-13 | 68.9 |
| MsG0880047611.01.T01 | MTR_2g018320 | 27.895 | 190 | 113 | 3 | 24 | 197 | 375 | 556 | 7.56e-13 | 67.8 |
| MsG0880047611.01.T01 | MTR_4g124040 | 27.907 | 172 | 99 | 3 | 24 | 178 | 1105 | 1268 | 1.71e-12 | 66.6 |
| MsG0880047611.01.T01 | MTR_4g124040 | 28.235 | 170 | 98 | 3 | 25 | 178 | 445 | 606 | 2.00e-12 | 66.6 |
| MsG0880047611.01.T01 | MTR_4g124040 | 27.907 | 172 | 99 | 3 | 24 | 178 | 873 | 1036 | 1.73e-12 | 66.6 |
| MsG0880047611.01.T01 | MTR_4g124040 | 28.235 | 170 | 98 | 3 | 25 | 178 | 213 | 374 | 2.06e-12 | 66.6 |
| MsG0880047611.01.T01 | MTR_4g124040 | 27.907 | 172 | 99 | 3 | 24 | 178 | 1050 | 1213 | 2.11e-12 | 66.6 |
| MsG0880047611.01.T01 | MTR_4g124040 | 28.235 | 170 | 98 | 3 | 25 | 178 | 390 | 551 | 2.32e-12 | 66.2 |
| MsG0880047611.01.T01 | MTR_4g124050 | 26.455 | 189 | 115 | 2 | 25 | 197 | 413 | 593 | 2.91e-12 | 65.9 |
| MsG0880047611.01.T01 | MTR_2g018530 | 28.070 | 171 | 99 | 3 | 24 | 178 | 385 | 547 | 3.67e-12 | 65.9 |
| MsG0880047611.01.T01 | MTR_7g102070 | 27.647 | 170 | 99 | 3 | 25 | 178 | 443 | 604 | 5.85e-12 | 65.1 |
| MsG0880047611.01.T01 | MTR_6g078080 | 28.409 | 176 | 98 | 4 | 24 | 183 | 381 | 544 | 6.08e-12 | 65.1 |
| MsG0880047611.01.T01 | MTR_4g081190 | 29.082 | 196 | 111 | 3 | 20 | 197 | 355 | 540 | 1.65e-11 | 63.9 |
| MsG0880047611.01.T01 | MTR_3g080220 | 28.488 | 172 | 98 | 2 | 24 | 178 | 1069 | 1232 | 2.40e-11 | 63.5 |
| MsG0880047611.01.T01 | MTR_2g018350 | 25.773 | 194 | 120 | 3 | 20 | 197 | 371 | 556 | 2.85e-11 | 63.2 |
| MsG0880047611.01.T01 | MTR_2g018350 | 25.131 | 191 | 118 | 2 | 24 | 197 | 1032 | 1214 | 9.27e-11 | 61.6 |
| MsG0880047611.01.T01 | MTR_0019s0020 | 31.915 | 188 | 99 | 8 | 21 | 196 | 583 | 753 | 4.25e-11 | 62.8 |
| MsG0880047611.01.T01 | MTR_4g077930 | 26.455 | 189 | 115 | 3 | 25 | 197 | 393 | 573 | 4.58e-11 | 62.4 |
| MsG0880047611.01.T01 | MTR_0019s0020 | 31.915 | 188 | 99 | 8 | 21 | 196 | 583 | 753 | 5.07e-11 | 62.4 |
| MsG0880047611.01.T01 | MTR_0019s0020 | 31.915 | 188 | 99 | 8 | 21 | 196 | 583 | 753 | 5.51e-11 | 62.4 |
| MsG0880047611.01.T01 | MTR_0019s0020 | 31.915 | 188 | 99 | 8 | 21 | 196 | 583 | 753 | 8.36e-11 | 61.6 |
| MsG0880047611.01.T01 | MTR_0019s0020 | 31.915 | 188 | 99 | 8 | 21 | 196 | 583 | 753 | 8.39e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047611.01.T01 | AT1G63270 | 83.406 | 229 | 38 | 0 | 1 | 229 | 1 | 229 | 5.38e-140 | 391 |
| MsG0880047611.01.T01 | AT1G67940 | 29.534 | 193 | 123 | 4 | 9 | 193 | 27 | 214 | 8.24e-15 | 72.0 |
| MsG0880047611.01.T01 | AT3G62150 | 28.431 | 204 | 119 | 4 | 13 | 197 | 405 | 600 | 2.86e-14 | 72.0 |
| MsG0880047611.01.T01 | AT3G62150 | 27.273 | 176 | 103 | 2 | 20 | 178 | 1064 | 1231 | 1.70e-13 | 69.7 |
| MsG0880047611.01.T01 | AT3G62150 | 28.431 | 204 | 119 | 4 | 13 | 197 | 405 | 600 | 2.86e-14 | 72.0 |
| MsG0880047611.01.T01 | AT3G62150 | 27.273 | 176 | 103 | 2 | 20 | 178 | 1064 | 1231 | 1.70e-13 | 69.7 |
| MsG0880047611.01.T01 | AT3G62150 | 28.431 | 204 | 119 | 4 | 13 | 197 | 405 | 600 | 2.86e-14 | 72.0 |
| MsG0880047611.01.T01 | AT3G62150 | 27.273 | 176 | 103 | 2 | 20 | 178 | 1064 | 1231 | 1.70e-13 | 69.7 |
| MsG0880047611.01.T01 | AT1G02530 | 28.652 | 178 | 100 | 3 | 20 | 178 | 1039 | 1208 | 1.04e-13 | 70.5 |
| MsG0880047611.01.T01 | AT1G02530 | 26.804 | 194 | 118 | 3 | 20 | 197 | 380 | 565 | 1.50e-13 | 70.1 |
| MsG0880047611.01.T01 | AT1G02530 | 26.804 | 194 | 118 | 3 | 20 | 197 | 380 | 565 | 1.44e-13 | 70.1 |
| MsG0880047611.01.T01 | AT4G18050 | 27.895 | 190 | 113 | 3 | 24 | 197 | 371 | 552 | 1.82e-13 | 69.7 |
| MsG0880047611.01.T01 | AT4G18050 | 25.989 | 177 | 106 | 2 | 19 | 178 | 1004 | 1172 | 4.71e-11 | 62.4 |
| MsG0880047611.01.T01 | AT4G18050 | 27.895 | 190 | 113 | 3 | 24 | 197 | 381 | 562 | 2.08e-13 | 69.3 |
| MsG0880047611.01.T01 | AT4G18050 | 25.989 | 177 | 106 | 2 | 19 | 178 | 1014 | 1182 | 5.28e-11 | 62.4 |
| MsG0880047611.01.T01 | AT5G46540 | 28.070 | 171 | 99 | 3 | 24 | 178 | 373 | 535 | 4.99e-13 | 68.6 |
| MsG0880047611.01.T01 | AT5G46540 | 26.923 | 208 | 126 | 3 | 19 | 209 | 1016 | 1214 | 5.33e-13 | 68.2 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 7.35e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 7.36e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 26.744 | 172 | 101 | 2 | 24 | 178 | 1058 | 1221 | 3.16e-12 | 65.9 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 7.36e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 26.744 | 172 | 101 | 2 | 24 | 178 | 1058 | 1221 | 3.16e-12 | 65.9 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 7.36e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 26.744 | 172 | 101 | 2 | 24 | 178 | 1058 | 1221 | 3.16e-12 | 65.9 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 8.24e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 8.24e-13 | 67.8 |
| MsG0880047611.01.T01 | AT2G47000 | 28.655 | 171 | 98 | 3 | 24 | 178 | 400 | 562 | 8.62e-13 | 67.8 |
| MsG0880047611.01.T01 | AT1G28010 | 25.926 | 189 | 116 | 3 | 25 | 197 | 389 | 569 | 1.48e-12 | 67.0 |
| MsG0880047611.01.T01 | AT4G01830 | 28.571 | 175 | 101 | 3 | 20 | 178 | 131 | 297 | 1.50e-12 | 67.0 |
| MsG0880047611.01.T01 | AT4G01830 | 28.324 | 173 | 98 | 3 | 24 | 178 | 767 | 931 | 4.96e-11 | 62.4 |
| MsG0880047611.01.T01 | AT4G01830 | 28.571 | 175 | 101 | 3 | 20 | 178 | 365 | 531 | 1.74e-12 | 66.6 |
| MsG0880047611.01.T01 | AT4G01830 | 28.324 | 173 | 98 | 3 | 24 | 178 | 1001 | 1165 | 6.24e-11 | 62.0 |
| MsG0880047611.01.T01 | AT1G02520 | 28.070 | 171 | 99 | 3 | 24 | 178 | 397 | 559 | 2.25e-12 | 66.6 |
| MsG0880047611.01.T01 | AT1G02520 | 27.011 | 174 | 100 | 2 | 24 | 178 | 1048 | 1213 | 8.37e-12 | 64.7 |
| MsG0880047611.01.T01 | AT1G02520 | 28.070 | 171 | 99 | 3 | 24 | 178 | 397 | 559 | 2.25e-12 | 66.6 |
| MsG0880047611.01.T01 | AT1G02520 | 27.011 | 174 | 100 | 2 | 24 | 178 | 1048 | 1213 | 8.37e-12 | 64.7 |
| MsG0880047611.01.T01 | AT1G02520 | 28.070 | 171 | 99 | 3 | 24 | 178 | 397 | 559 | 2.25e-12 | 66.6 |
| MsG0880047611.01.T01 | AT1G02520 | 27.011 | 174 | 100 | 2 | 24 | 178 | 1048 | 1213 | 8.37e-12 | 64.7 |
| MsG0880047611.01.T01 | AT1G27940 | 28.804 | 184 | 117 | 5 | 25 | 197 | 174 | 354 | 4.29e-12 | 65.5 |
| MsG0880047611.01.T01 | AT1G27940 | 28.042 | 189 | 112 | 3 | 25 | 197 | 807 | 987 | 7.53e-11 | 62.0 |
| MsG0880047611.01.T01 | AT1G27940 | 28.649 | 185 | 118 | 5 | 25 | 198 | 388 | 569 | 5.79e-12 | 65.1 |
| MsG0880047611.01.T01 | AT1G27940 | 27.895 | 190 | 113 | 3 | 25 | 198 | 1021 | 1202 | 9.51e-11 | 61.6 |
| MsG0880047611.01.T01 | AT4G01820 | 27.684 | 177 | 100 | 3 | 20 | 178 | 360 | 526 | 1.23e-11 | 64.3 |
| MsG0880047611.01.T01 | AT4G01820 | 27.684 | 177 | 100 | 3 | 20 | 178 | 360 | 526 | 1.37e-11 | 64.3 |
| MsG0880047611.01.T01 | AT2G36910 | 27.485 | 171 | 100 | 2 | 24 | 178 | 384 | 546 | 1.61e-11 | 63.9 |
| MsG0880047611.01.T01 | AT3G28380 | 28.000 | 175 | 102 | 3 | 20 | 178 | 371 | 537 | 3.50e-11 | 62.8 |
| MsG0880047611.01.T01 | AT3G28860 | 27.513 | 189 | 110 | 4 | 25 | 196 | 1027 | 1205 | 4.77e-11 | 62.4 |
Find 66 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTATAAGCTTCAGCTTAAC+TGG | 0.248424 | 8:+88788252 | MsG0880047611.01.T01:CDS |
| ACTTTCATGCGCATGTTAGC+TGG | 0.296912 | 8:+88788158 | MsG0880047611.01.T01:CDS |
| TGGCCCATTGAAAGCATCTT+AGG | 0.319230 | 8:-88788413 | None:intergenic |
| TAATGAGAAGTCTACTGTTC+TGG | 0.350793 | 8:+88788295 | MsG0880047611.01.T01:CDS |
| TATTGCAGAACACAGGAAAC+AGG | 0.372464 | 8:+88788547 | MsG0880047611.01.T01:CDS |
| TAAGCTTGCACTTGAGTTGA+TGG | 0.405805 | 8:+88788364 | MsG0880047611.01.T01:CDS |
| CGTCTGCAGGAGAAATCCTT+TGG | 0.429605 | 8:+88788192 | MsG0880047611.01.T01:CDS |
| CTTGTGCTAACAGGTGCAAA+TGG | 0.445397 | 8:+88788122 | MsG0880047611.01.T01:CDS |
| TGCACTTGAGTTGATGGGAC+TGG | 0.458658 | 8:+88788370 | MsG0880047611.01.T01:CDS |
| GGCTCGTCTAGCAACCATAT+GGG | 0.461520 | 8:-88788479 | None:intergenic |
| CATGGAATCTTCTATCTCAA+TGG | 0.462552 | 8:-88788600 | None:intergenic |
| GCACTTGAGTTGATGGGACT+GGG | 0.464272 | 8:+88788371 | MsG0880047611.01.T01:CDS |
| GGAGGGGCACTTGTGCTAAC+AGG | 0.470048 | 8:+88788113 | MsG0880047611.01.T01:CDS |
| GCGATCAATTGCGAGCAATC+TGG | 0.470226 | 8:-88788456 | None:intergenic |
| ATCCTCCTTGGAAACCTTGG+TGG | 0.473105 | 8:-88788635 | None:intergenic |
| TCTATAAGTGTCATCCTCCT+TGG | 0.473498 | 8:-88788647 | None:intergenic |
| TCGCAATTGATCGCCCCATA+TGG | 0.474072 | 8:+88788465 | MsG0880047611.01.T01:CDS |
| AGCTAACATGCGCATGAAAG+TGG | 0.475025 | 8:-88788156 | None:intergenic |
| AGGCTCGTCTAGCAACCATA+TGG | 0.475048 | 8:-88788480 | None:intergenic |
| CTTTCATGCGCATGTTAGCT+GGG | 0.479202 | 8:+88788159 | MsG0880047611.01.T01:CDS |
| CACTTATAGATATGCTTGAC+CGG | 0.480974 | 8:+88788660 | MsG0880047611.01.T01:CDS |
| ATGGAATCTTCTATCTCAAT+GGG | 0.487729 | 8:-88788599 | None:intergenic |
| GTTGATGGGACTGGGAAGAT+TGG | 0.488958 | 8:+88788379 | MsG0880047611.01.T01:CDS |
| AAAGCCTAAGATGCTTTCAA+TGG | 0.493892 | 8:+88788409 | MsG0880047611.01.T01:CDS |
| TCATGCAAGAGACATTGTTA+AGG | 0.501277 | 8:-88788043 | None:intergenic |
| GTCATCCTCCTTGGAAACCT+TGG | 0.511121 | 8:-88788638 | None:intergenic |
| TGCTCTTGAGGCTGCCACCA+AGG | 0.526833 | 8:+88788621 | MsG0880047611.01.T01:CDS |
| GGCTGCCACCAAGGTTTCCA+AGG | 0.535052 | 8:+88788630 | MsG0880047611.01.T01:CDS |
| GAATGTCATGGCCATTCCAA+AGG | 0.538492 | 8:-88788208 | None:intergenic |
| AATGTCTCTCTTCATGATGG+AGG | 0.538799 | 8:+88788095 | MsG0880047611.01.T01:CDS |
| AAGCCTAAGATGCTTTCAAT+GGG | 0.539281 | 8:+88788410 | MsG0880047611.01.T01:CDS |
| TCAGTTGCATTAGATGATGA+AGG | 0.540951 | 8:+88788503 | MsG0880047611.01.T01:CDS |
| GCTAACAGGTGCAAATGGCT+CGG | 0.541340 | 8:+88788127 | MsG0880047611.01.T01:CDS |
| CTAACAGGTGCAAATGGCTC+GGG | 0.543916 | 8:+88788128 | MsG0880047611.01.T01:CDS |
| ACATTGTTAAGGAGGAGACG+AGG | 0.550534 | 8:-88788032 | None:intergenic |
| TAAGCTGAAGCTTATACTGA+TGG | 0.554659 | 8:-88788247 | None:intergenic |
| AAGCTTGCACTTGAGTTGAT+GGG | 0.556728 | 8:+88788365 | MsG0880047611.01.T01:CDS |
| ATGTCTCTCTTCATGATGGA+GGG | 0.559238 | 8:+88788096 | MsG0880047611.01.T01:CDS |
| AACTGGGTCTGCCTCAAGCA+TGG | 0.564032 | 8:+88788269 | MsG0880047611.01.T01:CDS |
| TGGTGGCAGCCTCAAGAGCA+TGG | 0.568725 | 8:-88788618 | None:intergenic |
| TCTCATTAATTCCATGCTTG+AGG | 0.569583 | 8:-88788280 | None:intergenic |
| TGTCTCTCTTCATGATGGAG+GGG | 0.571601 | 8:+88788097 | MsG0880047611.01.T01:CDS |
| TCTTGATTACAAACGAGGGA+AGG | 0.572184 | 8:+88788340 | MsG0880047611.01.T01:CDS |
| GTGAATGTCTCTCTTCATGA+TGG | 0.575226 | 8:+88788092 | MsG0880047611.01.T01:CDS |
| TAGAAGATTCCATGCTCTTG+AGG | 0.587588 | 8:+88788609 | MsG0880047611.01.T01:CDS |
| AAAGTTAACTAATATCTGCC+CGG | 0.588067 | 8:-88788679 | None:intergenic |
| CACATTAGACAGAACTTGCG+AGG | 0.590033 | 8:-88788072 | None:intergenic |
| GCAGGAGAAATCCTTTGGAA+TGG | 0.592243 | 8:+88788197 | MsG0880047611.01.T01:CDS |
| TGCAAGAGACATTGTTAAGG+AGG | 0.593226 | 8:-88788040 | None:intergenic |
| GGGACTGGGAAGATTGGCTA+AGG | 0.595370 | 8:+88788385 | MsG0880047611.01.T01:CDS |
| CAAAGGATTTCTCCTGCAGA+CGG | 0.597971 | 8:-88788191 | None:intergenic |
| CATTGTTAAGGAGGAGACGA+GGG | 0.610651 | 8:-88788031 | None:intergenic |
| GTATAAGCTTCAGCTTAACT+GGG | 0.615494 | 8:+88788253 | MsG0880047611.01.T01:CDS |
| ACAGGGAGGAATTGTGATCG+TGG | 0.621551 | 8:+88788565 | MsG0880047611.01.T01:CDS |
| TCATCATCTAATGCAACTGA+AGG | 0.627900 | 8:-88788500 | None:intergenic |
| AACTTCTTGATTACAAACGA+GGG | 0.639263 | 8:+88788336 | MsG0880047611.01.T01:CDS |
| GCTCGTCTAGCAACCATATG+GGG | 0.642137 | 8:-88788478 | None:intergenic |
| GAACTTCTTGATTACAAACG+AGG | 0.642628 | 8:+88788335 | MsG0880047611.01.T01:CDS |
| ACTTATAGATATGCTTGACC+GGG | 0.653298 | 8:+88788661 | MsG0880047611.01.T01:CDS |
| ATTGCAGAACACAGGAAACA+GGG | 0.655611 | 8:+88788548 | MsG0880047611.01.T01:CDS |
| AGTACATTATTGCAGAACAC+AGG | 0.673742 | 8:+88788540 | MsG0880047611.01.T01:CDS |
| ATTGTTAAGGAGGAGACGAG+GGG | 0.674115 | 8:-88788030 | None:intergenic |
| AGATGCTTTCAATGGGCCAG+CGG | 0.680942 | 8:+88788417 | MsG0880047611.01.T01:CDS |
| TGCCACCAAGGTTTCCAAGG+AGG | 0.687258 | 8:+88788633 | MsG0880047611.01.T01:CDS |
| TTGTTAAGGAGGAGACGAGG+GGG | 0.688535 | 8:-88788029 | None:intergenic |
| GCAGAACACAGGAAACAGGG+AGG | 0.788797 | 8:+88788551 | MsG0880047611.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGTAGATTTTTGAATGTCA+TGG | - | Chr8:88788223-88788242 | None:intergenic | 25.0% |
| AACTTCTTGATTACAAACGA+GGG | + | Chr8:88788336-88788355 | MsG0880047611.01.T01:CDS | 30.0% | |
| ATGGAATCTTCTATCTCAAT+GGG | - | Chr8:88788602-88788621 | None:intergenic | 30.0% | |
| AAAGCCTAAGATGCTTTCAA+TGG | + | Chr8:88788409-88788428 | MsG0880047611.01.T01:CDS | 35.0% | |
| AAGCCTAAGATGCTTTCAAT+GGG | + | Chr8:88788410-88788429 | MsG0880047611.01.T01:CDS | 35.0% | |
| ACTTATAGATATGCTTGACC+GGG | + | Chr8:88788661-88788680 | MsG0880047611.01.T01:CDS | 35.0% | |
| AGTACATTATTGCAGAACAC+AGG | + | Chr8:88788540-88788559 | MsG0880047611.01.T01:CDS | 35.0% | |
| AGTATAAGCTTCAGCTTAAC+TGG | + | Chr8:88788252-88788271 | MsG0880047611.01.T01:CDS | 35.0% | |
| CACTTATAGATATGCTTGAC+CGG | + | Chr8:88788660-88788679 | MsG0880047611.01.T01:CDS | 35.0% | |
| CATGGAATCTTCTATCTCAA+TGG | - | Chr8:88788603-88788622 | None:intergenic | 35.0% | |
| GAACTTCTTGATTACAAACG+AGG | + | Chr8:88788335-88788354 | MsG0880047611.01.T01:CDS | 35.0% | |
| GTATAAGCTTCAGCTTAACT+GGG | + | Chr8:88788253-88788272 | MsG0880047611.01.T01:CDS | 35.0% | |
| TAAGCTGAAGCTTATACTGA+TGG | - | Chr8:88788250-88788269 | None:intergenic | 35.0% | |
| TAATGAGAAGTCTACTGTTC+TGG | + | Chr8:88788295-88788314 | MsG0880047611.01.T01:CDS | 35.0% | |
| TCAGTTGCATTAGATGATGA+AGG | + | Chr8:88788503-88788522 | MsG0880047611.01.T01:CDS | 35.0% | |
| TCATCATCTAATGCAACTGA+AGG | - | Chr8:88788503-88788522 | None:intergenic | 35.0% | |
| TCATGCAAGAGACATTGTTA+AGG | - | Chr8:88788046-88788065 | None:intergenic | 35.0% | |
| TCTCATTAATTCCATGCTTG+AGG | - | Chr8:88788283-88788302 | None:intergenic | 35.0% | |
| AATGTCTCTCTTCATGATGG+AGG | + | Chr8:88788095-88788114 | MsG0880047611.01.T01:CDS | 40.0% | |
| ATTGCAGAACACAGGAAACA+GGG | + | Chr8:88788548-88788567 | MsG0880047611.01.T01:CDS | 40.0% | |
| GTGAATGTCTCTCTTCATGA+TGG | + | Chr8:88788092-88788111 | MsG0880047611.01.T01:CDS | 40.0% | |
| TAGAAGATTCCATGCTCTTG+AGG | + | Chr8:88788609-88788628 | MsG0880047611.01.T01:CDS | 40.0% | |
| TATTGCAGAACACAGGAAAC+AGG | + | Chr8:88788547-88788566 | MsG0880047611.01.T01:CDS | 40.0% | |
| TCTATAAGTGTCATCCTCCT+TGG | - | Chr8:88788650-88788669 | None:intergenic | 40.0% | |
| TCTTGATTACAAACGAGGGA+AGG | + | Chr8:88788340-88788359 | MsG0880047611.01.T01:CDS | 40.0% | |
| TGCAAGAGACATTGTTAAGG+AGG | - | Chr8:88788043-88788062 | None:intergenic | 40.0% | |
| ! | ATGTCTCTCTTCATGATGGA+GGG | + | Chr8:88788096-88788115 | MsG0880047611.01.T01:CDS | 40.0% |
| !! | AAGCTTGCACTTGAGTTGAT+GGG | + | Chr8:88788365-88788384 | MsG0880047611.01.T01:CDS | 40.0% |
| !! | TAAGCTTGCACTTGAGTTGA+TGG | + | Chr8:88788364-88788383 | MsG0880047611.01.T01:CDS | 40.0% |
| ACATTGTTAAGGAGGAGACG+AGG | - | Chr8:88788035-88788054 | None:intergenic | 45.0% | |
| ACTTTCATGCGCATGTTAGC+TGG | + | Chr8:88788158-88788177 | MsG0880047611.01.T01:CDS | 45.0% | |
| AGCTAACATGCGCATGAAAG+TGG | - | Chr8:88788159-88788178 | None:intergenic | 45.0% | |
| ATTGTTAAGGAGGAGACGAG+GGG | - | Chr8:88788033-88788052 | None:intergenic | 45.0% | |
| CAAAGGATTTCTCCTGCAGA+CGG | - | Chr8:88788194-88788213 | None:intergenic | 45.0% | |
| CACATTAGACAGAACTTGCG+AGG | - | Chr8:88788075-88788094 | None:intergenic | 45.0% | |
| CATTGTTAAGGAGGAGACGA+GGG | - | Chr8:88788034-88788053 | None:intergenic | 45.0% | |
| CTTGTGCTAACAGGTGCAAA+TGG | + | Chr8:88788122-88788141 | MsG0880047611.01.T01:CDS | 45.0% | |
| CTTTCATGCGCATGTTAGCT+GGG | + | Chr8:88788159-88788178 | MsG0880047611.01.T01:CDS | 45.0% | |
| GAATGTCATGGCCATTCCAA+AGG | - | Chr8:88788211-88788230 | None:intergenic | 45.0% | |
| ! | CAAGTTGCAATCTTTTCCGC+TGG | - | Chr8:88788436-88788455 | None:intergenic | 45.0% |
| ! | GCAGGAGAAATCCTTTGGAA+TGG | + | Chr8:88788197-88788216 | MsG0880047611.01.T01:CDS | 45.0% |
| ! | TGTCTCTCTTCATGATGGAG+GGG | + | Chr8:88788097-88788116 | MsG0880047611.01.T01:CDS | 45.0% |
| !! | TGGCCCATTGAAAGCATCTT+AGG | - | Chr8:88788416-88788435 | None:intergenic | 45.0% |
| ACAGGGAGGAATTGTGATCG+TGG | + | Chr8:88788565-88788584 | MsG0880047611.01.T01:CDS | 50.0% | |
| AGATGCTTTCAATGGGCCAG+CGG | + | Chr8:88788417-88788436 | MsG0880047611.01.T01:CDS | 50.0% | |
| AGGCTCGTCTAGCAACCATA+TGG | - | Chr8:88788483-88788502 | None:intergenic | 50.0% | |
| ATCCTCCTTGGAAACCTTGG+TGG | - | Chr8:88788638-88788657 | None:intergenic | 50.0% | |
| CGTCTGCAGGAGAAATCCTT+TGG | + | Chr8:88788192-88788211 | MsG0880047611.01.T01:CDS | 50.0% | |
| CTAACAGGTGCAAATGGCTC+GGG | + | Chr8:88788128-88788147 | MsG0880047611.01.T01:CDS | 50.0% | |
| GCGATCAATTGCGAGCAATC+TGG | - | Chr8:88788459-88788478 | None:intergenic | 50.0% | |
| GCTAACAGGTGCAAATGGCT+CGG | + | Chr8:88788127-88788146 | MsG0880047611.01.T01:CDS | 50.0% | |
| GCTCGTCTAGCAACCATATG+GGG | - | Chr8:88788481-88788500 | None:intergenic | 50.0% | |
| GGCTCGTCTAGCAACCATAT+GGG | - | Chr8:88788482-88788501 | None:intergenic | 50.0% | |
| GTCATCCTCCTTGGAAACCT+TGG | - | Chr8:88788641-88788660 | None:intergenic | 50.0% | |
| TCGCAATTGATCGCCCCATA+TGG | + | Chr8:88788465-88788484 | MsG0880047611.01.T01:CDS | 50.0% | |
| TTGTTAAGGAGGAGACGAGG+GGG | - | Chr8:88788032-88788051 | None:intergenic | 50.0% | |
| ! | GTTGATGGGACTGGGAAGAT+TGG | + | Chr8:88788379-88788398 | MsG0880047611.01.T01:CDS | 50.0% |
| !! | GCACTTGAGTTGATGGGACT+GGG | + | Chr8:88788371-88788390 | MsG0880047611.01.T01:CDS | 50.0% |
| !! | TGCACTTGAGTTGATGGGAC+TGG | + | Chr8:88788370-88788389 | MsG0880047611.01.T01:CDS | 50.0% |
| AACTGGGTCTGCCTCAAGCA+TGG | + | Chr8:88788269-88788288 | MsG0880047611.01.T01:CDS | 55.0% | |
| GCAGAACACAGGAAACAGGG+AGG | + | Chr8:88788551-88788570 | MsG0880047611.01.T01:CDS | 55.0% | |
| TGCCACCAAGGTTTCCAAGG+AGG | + | Chr8:88788633-88788652 | MsG0880047611.01.T01:CDS | 55.0% | |
| ! | GGGACTGGGAAGATTGGCTA+AGG | + | Chr8:88788385-88788404 | MsG0880047611.01.T01:CDS | 55.0% |
| GGCTGCCACCAAGGTTTCCA+AGG | + | Chr8:88788630-88788649 | MsG0880047611.01.T01:CDS | 60.0% | |
| TGCTCTTGAGGCTGCCACCA+AGG | + | Chr8:88788621-88788640 | MsG0880047611.01.T01:CDS | 60.0% | |
| TGGTGGCAGCCTCAAGAGCA+TGG | - | Chr8:88788621-88788640 | None:intergenic | 60.0% | |
| TTAAGGAGGAGACGAGGGGG+TGG | - | Chr8:88788029-88788048 | None:intergenic | 60.0% | |
| ! | GGGTTTTCTCGTCCGTCTGC+AGG | + | Chr8:88788179-88788198 | MsG0880047611.01.T01:CDS | 60.0% |
| !! | GGAGGGGCACTTGTGCTAAC+AGG | + | Chr8:88788113-88788132 | MsG0880047611.01.T01:CDS | 60.0% |
| AGGAGGAGACGAGGGGGTGG+AGG | - | Chr8:88788026-88788045 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 88788008 | 88788697 | 88788008 | ID=MsG0880047611.01;Name=MsG0880047611.01 |
| Chr8 | mRNA | 88788008 | 88788697 | 88788008 | ID=MsG0880047611.01.T01;Parent=MsG0880047611.01;Name=MsG0880047611.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|229 |
| Chr8 | exon | 88788008 | 88788697 | 88788008 | ID=MsG0880047611.01.T01:exon:16019;Parent=MsG0880047611.01.T01 |
| Chr8 | CDS | 88788008 | 88788697 | 88788008 | ID=MsG0880047611.01.T01:cds;Parent=MsG0880047611.01.T01 |
| Gene Sequence |
| Protein sequence |
