Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0029810 | 0.816948 | 4.250309e-52 | -8.615850e-47 |
| Msa0029810 | Msa0157290 | 0.816964 | 4.215749e-52 | -8.615850e-47 |
| Msa0029810 | Msa0243100 | 0.808980 | 2.372231e-50 | -8.615850e-47 |
| Msa0029810 | Msa0437990 | 0.802746 | 4.846878e-49 | -8.615850e-47 |
| Msa0029810 | Msa0512900 | 0.808878 | 2.494335e-50 | -8.615850e-47 |
| Msa0029810 | Msa0661870 | 0.809601 | 1.746068e-50 | -8.615850e-47 |
| Msa0029810 | Msa0880140 | 0.814526 | 1.472811e-51 | -8.615850e-47 |
| Msa0029810 | Msa0880340 | 0.963666 | 2.295254e-122 | -8.615850e-47 |
| Msa0029810 | Msa1014540 | 0.813018 | 3.165041e-51 | -8.615850e-47 |
| Msa0029810 | Msa1085390 | 0.937402 | 3.671213e-98 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGAGAATGTCATTAAATT+TGG | 0.171054 | 1_1:+55273274 | None:intergenic |
| CTTCTGCATATGGATCCTTT+TGG | 0.279760 | 1_1:-55273243 | Msa0029810:CDS |
| GTTTATTACAGAGTTGGATA+TGG | 0.319512 | 1_1:-55273340 | Msa0029810:intron |
| GGGGAGGATGCTTGAATAAG+AGG | 0.376419 | 1_1:+55273206 | None:intergenic |
| GTATAATTGTCTTCTGCATA+TGG | 0.387682 | 1_1:-55273253 | Msa0029810:CDS |
| TATGGAAAAGTGGTTGGAGC+TGG | 0.462385 | 1_1:-55274058 | Msa0029810:intron |
| TTTAGTGTATAGTTAGAAAG+GGG | 0.507137 | 1_1:+55273187 | None:intergenic |
| ATGCATAGTTTGCTAACTTG+TGG | 0.540764 | 1_1:+55274102 | None:intergenic |
| AGTGTATAGTTAGAAAGGGG+AGG | 0.548704 | 1_1:+55273190 | None:intergenic |
| ACAAGTTAGCAAACTATGCA+TGG | 0.605098 | 1_1:-55274100 | Msa0029810:CDS |
| AGTTAGCAAACTATGCATGG+CGG | 0.782698 | 1_1:-55274097 | Msa0029810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTGAATTGATTTTTTTAAA+GGG | - | chr1_1:55273686-55273705 | Msa0029810:intron | 15.0% |
| !!! | CTTGAATTGATTTTTTTAAA+GGG | - | chr1_1:55273686-55273705 | Msa0029810:intron | 15.0% |
| !!! | AATTCATTTTTATGGAAAAG+TGG | - | chr1_1:55273537-55273556 | Msa0029810:intron | 20.0% |
| !!! | CCTTTAAAAAAATCAATTCA+AGG | + | chr1_1:55273688-55273707 | None:intergenic | 20.0% |
| !!! | CCTTGAATTGATTTTTTTAA+AGG | - | chr1_1:55273685-55273704 | Msa0029810:intron | 20.0% |
| !! | ATAATAACTACACACAAAAA+AGG | + | chr1_1:55273874-55273893 | None:intergenic | 20.0% |
| !!! | TTATTTCTTAAACCTATTTG+TGG | - | chr1_1:55273903-55273922 | Msa0029810:intron | 20.0% |
| !!! | ACAATTATTATAGTTGGTTT+TGG | - | chr1_1:55274105-55274124 | Msa0029810:CDS | 20.0% |
| !!! | AATTCATTTTTATGGAAAAG+TGG | - | chr1_1:55273537-55273556 | Msa0029810:intron | 20.0% |
| !!! | CCTTTAAAAAAATCAATTCA+AGG | + | chr1_1:55273688-55273707 | None:intergenic | 20.0% |
| !!! | CCTTGAATTGATTTTTTTAA+AGG | - | chr1_1:55273685-55273704 | Msa0029810:intron | 20.0% |
| !! | ATAATAACTACACACAAAAA+AGG | + | chr1_1:55273874-55273893 | None:intergenic | 20.0% |
| !!! | TTATTTCTTAAACCTATTTG+TGG | - | chr1_1:55273903-55273922 | Msa0029810:intron | 20.0% |
| !!! | ACAATTATTATAGTTGGTTT+TGG | - | chr1_1:55274105-55274124 | Msa0029810:CDS | 20.0% |
| ! | TAGAAGAAAAAGAAGTGAAT+GGG | + | chr1_1:55273241-55273260 | None:intergenic | 25.0% |
| ! | AATGAAGTCATGAATGTAAA+TGG | + | chr1_1:55273285-55273304 | None:intergenic | 25.0% |
| ! | ATGCAAAGAAGGAAAAAAAA+AGG | + | chr1_1:55273340-55273359 | None:intergenic | 25.0% |
| ! | ATTTAGATTTAGTGCTTATG+TGG | - | chr1_1:55273436-55273455 | Msa0029810:intron | 25.0% |
| ! | TGAAAATATTGCTATCATCT+TGG | - | chr1_1:55273463-55273482 | Msa0029810:intron | 25.0% |
| !!! | GGTTGAAGAATTCATTTTTA+TGG | - | chr1_1:55273529-55273548 | Msa0029810:intron | 25.0% |
| !! | TTCTCAAAGGTTTCTAAAAA+AGG | - | chr1_1:55273603-55273622 | Msa0029810:intron | 25.0% |
| !!! | TATCTTTAATCCACTTTTCA+TGG | - | chr1_1:55273712-55273731 | Msa0029810:intron | 25.0% |
| !! | TAATAGTTTCACAAGAGTTT+TGG | - | chr1_1:55273823-55273842 | Msa0029810:intron | 25.0% |
| ! | TCCTCGATTAAATGAATTTA+AGG | - | chr1_1:55274007-55274026 | Msa0029810:intron | 25.0% |
| ! | ACCTTAAATTCATTTAATCG+AGG | + | chr1_1:55274011-55274030 | None:intergenic | 25.0% |
| ! | CATGCAACAATTATTATAGT+TGG | - | chr1_1:55274099-55274118 | Msa0029810:CDS | 25.0% |
| !!! | TTCAAAGTATTTTCATGCTT+TGG | - | chr1_1:55274157-55274176 | Msa0029810:intron | 25.0% |
| ! | ACTGAGAATGTCATTAAATT+TGG | + | chr1_1:55274334-55274353 | None:intergenic | 25.0% |
| ! | TAGAAGAAAAAGAAGTGAAT+GGG | + | chr1_1:55273241-55273260 | None:intergenic | 25.0% |
| ! | AATGAAGTCATGAATGTAAA+TGG | + | chr1_1:55273285-55273304 | None:intergenic | 25.0% |
| ! | ATGCAAAGAAGGAAAAAAAA+AGG | + | chr1_1:55273340-55273359 | None:intergenic | 25.0% |
| ! | ATTTAGATTTAGTGCTTATG+TGG | - | chr1_1:55273436-55273455 | Msa0029810:intron | 25.0% |
| ! | TGAAAATATTGCTATCATCT+TGG | - | chr1_1:55273463-55273482 | Msa0029810:intron | 25.0% |
| !!! | GGTTGAAGAATTCATTTTTA+TGG | - | chr1_1:55273529-55273548 | Msa0029810:intron | 25.0% |
| !! | TTCTCAAAGGTTTCTAAAAA+AGG | - | chr1_1:55273603-55273622 | Msa0029810:intron | 25.0% |
| !!! | TATCTTTAATCCACTTTTCA+TGG | - | chr1_1:55273712-55273731 | Msa0029810:intron | 25.0% |
| !! | TAATAGTTTCACAAGAGTTT+TGG | - | chr1_1:55273823-55273842 | Msa0029810:intron | 25.0% |
| ! | TCCTCGATTAAATGAATTTA+AGG | - | chr1_1:55274007-55274026 | Msa0029810:intron | 25.0% |
| ! | ACCTTAAATTCATTTAATCG+AGG | + | chr1_1:55274011-55274030 | None:intergenic | 25.0% |
| ! | CATGCAACAATTATTATAGT+TGG | - | chr1_1:55274099-55274118 | Msa0029810:CDS | 25.0% |
| !!! | TTCAAAGTATTTTCATGCTT+TGG | - | chr1_1:55274157-55274176 | Msa0029810:intron | 25.0% |
| ! | ACTGAGAATGTCATTAAATT+TGG | + | chr1_1:55274334-55274353 | None:intergenic | 25.0% |
| GTAGAAGAAAAAGAAGTGAA+TGG | + | chr1_1:55273242-55273261 | None:intergenic | 30.0% | |
| TTTGTGATAAATGATTCTGC+AGG | + | chr1_1:55273376-55273395 | None:intergenic | 30.0% | |
| ! | CATTTTTATGGAAAAGTGGT+TGG | - | chr1_1:55273541-55273560 | Msa0029810:intron | 30.0% |
| GCTGAATTGAAATTTCTCAA+AGG | - | chr1_1:55273590-55273609 | Msa0029810:intron | 30.0% | |
| ATAGAACAATCCATGAAAAG+TGG | + | chr1_1:55273725-55273744 | None:intergenic | 30.0% | |
| AGATGCGACTATTTCATTAA+AGG | + | chr1_1:55273762-55273781 | None:intergenic | 30.0% | |
| !!! | AGTTGTTTTAGTGTCATTGT+TGG | - | chr1_1:55273799-55273818 | Msa0029810:intron | 30.0% |
| !!! | GTTGTTTTAGTGTCATTGTT+GGG | - | chr1_1:55273800-55273819 | Msa0029810:intron | 30.0% |
| ! | AAAAGAGTACAACCACAAAT+AGG | + | chr1_1:55273918-55273937 | None:intergenic | 30.0% |
| TTTGCATTCAAAATTGTCCT+TGG | + | chr1_1:55274072-55274091 | None:intergenic | 30.0% | |
| !!! | TTATTATAGTTGGTTTTGGC+TGG | - | chr1_1:55274109-55274128 | Msa0029810:CDS | 30.0% |
| !! | TTTCATGCTTTGGAATGTAA+AGG | - | chr1_1:55274167-55274186 | Msa0029810:intron | 30.0% |
| TCCTTAGTTTATTACAGAGT+TGG | - | chr1_1:55274259-55274278 | Msa0029810:intron | 30.0% | |
| TCCAACTCTGTAATAAACTA+AGG | + | chr1_1:55274263-55274282 | None:intergenic | 30.0% | |
| GTTTATTACAGAGTTGGATA+TGG | - | chr1_1:55274265-55274284 | Msa0029810:intron | 30.0% | |
| GTATAATTGTCTTCTGCATA+TGG | - | chr1_1:55274352-55274371 | Msa0029810:intron | 30.0% | |
| ! | GATTTAGTGATTTTCCCAAA+AGG | + | chr1_1:55274380-55274399 | None:intergenic | 30.0% |
| GTAGAAGAAAAAGAAGTGAA+TGG | + | chr1_1:55273242-55273261 | None:intergenic | 30.0% | |
| TTTGTGATAAATGATTCTGC+AGG | + | chr1_1:55273376-55273395 | None:intergenic | 30.0% | |
| ! | CATTTTTATGGAAAAGTGGT+TGG | - | chr1_1:55273541-55273560 | Msa0029810:intron | 30.0% |
| GCTGAATTGAAATTTCTCAA+AGG | - | chr1_1:55273590-55273609 | Msa0029810:intron | 30.0% | |
| ATAGAACAATCCATGAAAAG+TGG | + | chr1_1:55273725-55273744 | None:intergenic | 30.0% | |
| AGATGCGACTATTTCATTAA+AGG | + | chr1_1:55273762-55273781 | None:intergenic | 30.0% | |
| !!! | AGTTGTTTTAGTGTCATTGT+TGG | - | chr1_1:55273799-55273818 | Msa0029810:intron | 30.0% |
| !!! | GTTGTTTTAGTGTCATTGTT+GGG | - | chr1_1:55273800-55273819 | Msa0029810:intron | 30.0% |
| ! | AAAAGAGTACAACCACAAAT+AGG | + | chr1_1:55273918-55273937 | None:intergenic | 30.0% |
| TTTGCATTCAAAATTGTCCT+TGG | + | chr1_1:55274072-55274091 | None:intergenic | 30.0% | |
| !!! | TTATTATAGTTGGTTTTGGC+TGG | - | chr1_1:55274109-55274128 | Msa0029810:CDS | 30.0% |
| !! | TTTCATGCTTTGGAATGTAA+AGG | - | chr1_1:55274167-55274186 | Msa0029810:intron | 30.0% |
| TCCTTAGTTTATTACAGAGT+TGG | - | chr1_1:55274259-55274278 | Msa0029810:intron | 30.0% | |
| TCCAACTCTGTAATAAACTA+AGG | + | chr1_1:55274263-55274282 | None:intergenic | 30.0% | |
| GTTTATTACAGAGTTGGATA+TGG | - | chr1_1:55274265-55274284 | Msa0029810:intron | 30.0% | |
| GTATAATTGTCTTCTGCATA+TGG | - | chr1_1:55274352-55274371 | Msa0029810:intron | 30.0% | |
| ! | GATTTAGTGATTTTCCCAAA+AGG | + | chr1_1:55274380-55274399 | None:intergenic | 30.0% |
| ! | TTGAGATTTGAGATCAAGAG+AGG | + | chr1_1:55273314-55273333 | None:intergenic | 35.0% |
| TTTGATCTAGCATGCAAAGA+AGG | + | chr1_1:55273351-55273370 | None:intergenic | 35.0% | |
| !! | TGCTATCATCTTGGTTTGTT+AGG | - | chr1_1:55273472-55273491 | Msa0029810:intron | 35.0% |
| !! | ATGCATAGTTTGCTAACTTG+TGG | + | chr1_1:55273506-55273525 | None:intergenic | 35.0% |
| ACAAGTTAGCAAACTATGCA+TGG | - | chr1_1:55273505-55273524 | Msa0029810:intron | 35.0% | |
| TACACCTTGAATTGAAACCA+AGG | - | chr1_1:55273651-55273670 | Msa0029810:intron | 35.0% | |
| ACACCTTGAATTGAAACCAA+GGG | - | chr1_1:55273652-55273671 | Msa0029810:intron | 35.0% | |
| AAACCCTTGGTTTCAATTCA+AGG | + | chr1_1:55273658-55273677 | None:intergenic | 35.0% | |
| GATGGAAGTCCACAATTATA+TGG | - | chr1_1:55273974-55273993 | Msa0029810:intron | 35.0% | |
| !! | TAAAGGTATTGATGCTCTGT+TGG | - | chr1_1:55274184-55274203 | Msa0029810:intron | 35.0% |
| ! | TTCTGCATATGGATCCTTTT+GGG | - | chr1_1:55274363-55274382 | Msa0029810:intron | 35.0% |
| ! | TTGAGATTTGAGATCAAGAG+AGG | + | chr1_1:55273314-55273333 | None:intergenic | 35.0% |
| TTTGATCTAGCATGCAAAGA+AGG | + | chr1_1:55273351-55273370 | None:intergenic | 35.0% | |
| !! | TGCTATCATCTTGGTTTGTT+AGG | - | chr1_1:55273472-55273491 | Msa0029810:intron | 35.0% |
| !! | ATGCATAGTTTGCTAACTTG+TGG | + | chr1_1:55273506-55273525 | None:intergenic | 35.0% |
| ACAAGTTAGCAAACTATGCA+TGG | - | chr1_1:55273505-55273524 | Msa0029810:intron | 35.0% | |
| TACACCTTGAATTGAAACCA+AGG | - | chr1_1:55273651-55273670 | Msa0029810:intron | 35.0% | |
| ACACCTTGAATTGAAACCAA+GGG | - | chr1_1:55273652-55273671 | Msa0029810:intron | 35.0% | |
| AAACCCTTGGTTTCAATTCA+AGG | + | chr1_1:55273658-55273677 | None:intergenic | 35.0% | |
| GATGGAAGTCCACAATTATA+TGG | - | chr1_1:55273974-55273993 | Msa0029810:intron | 35.0% | |
| !! | TAAAGGTATTGATGCTCTGT+TGG | - | chr1_1:55274184-55274203 | Msa0029810:intron | 35.0% |
| ! | TTCTGCATATGGATCCTTTT+GGG | - | chr1_1:55274363-55274382 | Msa0029810:intron | 35.0% |
| GGCAAACGAAAATGTAGAAC+AGG | + | chr1_1:55273264-55273283 | None:intergenic | 40.0% | |
| AGTTAGCAAACTATGCATGG+CGG | - | chr1_1:55273508-55273527 | Msa0029810:intron | 40.0% | |
| TCAAGGCAAAGTAAAACCCT+TGG | + | chr1_1:55273671-55273690 | None:intergenic | 40.0% | |
| TGCACAAAGTCATGGTAGAA+AGG | + | chr1_1:55273943-55273962 | None:intergenic | 40.0% | |
| TCTACCATGACTTTGTGCAA+AGG | - | chr1_1:55273944-55273963 | Msa0029810:intron | 40.0% | |
| CATTCCTTTGCACAAAGTCA+TGG | + | chr1_1:55273951-55273970 | None:intergenic | 40.0% | |
| TGACTTTGTGCAAAGGAATG+TGG | - | chr1_1:55273951-55273970 | Msa0029810:intron | 40.0% | |
| GACTTTGTGCAAAGGAATGT+GGG | - | chr1_1:55273952-55273971 | Msa0029810:intron | 40.0% | |
| CTTGCAGCACCATATAATTG+TGG | + | chr1_1:55273986-55274005 | None:intergenic | 40.0% | |
| TTGGCTGGAATGAAAGACAA+TGG | - | chr1_1:55274124-55274143 | Msa0029810:intron | 40.0% | |
| ! | ATTGATGCTCTGTTGGTGTT+TGG | - | chr1_1:55274191-55274210 | Msa0029810:intron | 40.0% |
| ! | CTTCTGCATATGGATCCTTT+TGG | - | chr1_1:55274362-55274381 | Msa0029810:intron | 40.0% |
| GGCAAACGAAAATGTAGAAC+AGG | + | chr1_1:55273264-55273283 | None:intergenic | 40.0% | |
| AGTTAGCAAACTATGCATGG+CGG | - | chr1_1:55273508-55273527 | Msa0029810:intron | 40.0% | |
| TCAAGGCAAAGTAAAACCCT+TGG | + | chr1_1:55273671-55273690 | None:intergenic | 40.0% | |
| TGCACAAAGTCATGGTAGAA+AGG | + | chr1_1:55273943-55273962 | None:intergenic | 40.0% | |
| TCTACCATGACTTTGTGCAA+AGG | - | chr1_1:55273944-55273963 | Msa0029810:intron | 40.0% | |
| CATTCCTTTGCACAAAGTCA+TGG | + | chr1_1:55273951-55273970 | None:intergenic | 40.0% | |
| TGACTTTGTGCAAAGGAATG+TGG | - | chr1_1:55273951-55273970 | Msa0029810:intron | 40.0% | |
| GACTTTGTGCAAAGGAATGT+GGG | - | chr1_1:55273952-55273971 | Msa0029810:intron | 40.0% | |
| CTTGCAGCACCATATAATTG+TGG | + | chr1_1:55273986-55274005 | None:intergenic | 40.0% | |
| TTGGCTGGAATGAAAGACAA+TGG | - | chr1_1:55274124-55274143 | Msa0029810:intron | 40.0% | |
| ! | ATTGATGCTCTGTTGGTGTT+TGG | - | chr1_1:55274191-55274210 | Msa0029810:intron | 40.0% |
| ! | CTTCTGCATATGGATCCTTT+TGG | - | chr1_1:55274362-55274381 | Msa0029810:intron | 40.0% |
| ! | GATTTGAGATCAAGAGAGGC+TGG | + | chr1_1:55273310-55273329 | None:intergenic | 45.0% |
| TATGGAAAAGTGGTTGGAGC+TGG | - | chr1_1:55273547-55273566 | Msa0029810:intron | 45.0% | |
| TTGTGCAAAGGAATGTGGGA+TGG | - | chr1_1:55273956-55273975 | Msa0029810:intron | 45.0% | |
| ! | GATTTGAGATCAAGAGAGGC+TGG | + | chr1_1:55273310-55273329 | None:intergenic | 45.0% |
| TATGGAAAAGTGGTTGGAGC+TGG | - | chr1_1:55273547-55273566 | Msa0029810:intron | 45.0% | |
| TTGTGCAAAGGAATGTGGGA+TGG | - | chr1_1:55273956-55273975 | Msa0029810:intron | 45.0% | |
| GATTCATCGTCGTGAGACCA+AGG | - | chr1_1:55274052-55274071 | Msa0029810:intron | 50.0% | |
| GGGGAGGATGCTTGAATAAG+AGG | + | chr1_1:55274402-55274421 | None:intergenic | 50.0% | |
| GATTCATCGTCGTGAGACCA+AGG | - | chr1_1:55274052-55274071 | Msa0029810:intron | 50.0% | |
| GGGGAGGATGCTTGAATAAG+AGG | + | chr1_1:55274402-55274421 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 55273199 | 55274428 | 55273199 | ID=Msa0029810;Name=Msa0029810 |
| chr1_1 | mRNA | 55273199 | 55274428 | 55273199 | ID=Msa0029810-mRNA-1;Parent=Msa0029810;Name=Msa0029810-mRNA-1;_AED=0.56;_eAED=0.77;_QI=0|0|0|1|0|0|3|0|77 |
| chr1_1 | exon | 55274423 | 55274428 | 55274423 | ID=Msa0029810-mRNA-1:exon:16559;Parent=Msa0029810-mRNA-1 |
| chr1_1 | exon | 55274059 | 55274133 | 55274059 | ID=Msa0029810-mRNA-1:exon:16558;Parent=Msa0029810-mRNA-1 |
| chr1_1 | exon | 55273199 | 55273351 | 55273199 | ID=Msa0029810-mRNA-1:exon:16557;Parent=Msa0029810-mRNA-1 |
| chr1_1 | CDS | 55274423 | 55274428 | 55274423 | ID=Msa0029810-mRNA-1:cds;Parent=Msa0029810-mRNA-1 |
| chr1_1 | CDS | 55274059 | 55274133 | 55274059 | ID=Msa0029810-mRNA-1:cds;Parent=Msa0029810-mRNA-1 |
| chr1_1 | CDS | 55273199 | 55273351 | 55273199 | ID=Msa0029810-mRNA-1:cds;Parent=Msa0029810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
