AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1085390


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGAGAATGTCATTAAATT+TGG 0.171054 7_2:+86069532 None:intergenic
CTTCTGCATATGGATCCTTT+TGG 0.279760 7_2:-86069501 Msa1085390:CDS
GTTTATTACAGAGTTGGATA+TGG 0.319512 7_2:-86069598 Msa1085390:intron
GGGGAGGATGCTTGAATAAG+AGG 0.376419 7_2:+86069464 None:intergenic
GTATAATTGTCTTCTGCATA+TGG 0.387682 7_2:-86069511 Msa1085390:CDS
TATGGAAAAGTGGTTGGAGC+TGG 0.462385 7_2:-86070313 Msa1085390:intron
TTTAGTGTATAGTTAGAAAG+GGG 0.507137 7_2:+86069445 None:intergenic
ATGCATAGTTTGCTAACTTG+TGG 0.540764 7_2:+86070357 None:intergenic
AGTGTATAGTTAGAAAGGGG+AGG 0.548704 7_2:+86069448 None:intergenic
ACAAGTTAGCAAACTATGCA+TGG 0.605098 7_2:-86070355 Msa1085390:CDS
AGTTAGCAAACTATGCATGG+CGG 0.782698 7_2:-86070352 Msa1085390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTCATTTTTATGGAAAAG+TGG - chr7_2:86069795-86069814 Msa1085390:intron 20.0%
!!! CCTTGAATTGATTTTTTAAA+GGG - chr7_2:86069943-86069962 Msa1085390:intron 20.0%
!! ATAATAACTACACACAAAAA+AGG + chr7_2:86070129-86070148 None:intergenic 20.0%
!!! TTATTTCTTAAACCTATTTG+TGG - chr7_2:86070158-86070177 Msa1085390:intron 20.0%
!!! ACAATTATTATAGTTGGTTT+TGG - chr7_2:86070360-86070379 Msa1085390:CDS 20.0%
!!! AATTCATTTTTATGGAAAAG+TGG - chr7_2:86069795-86069814 Msa1085390:intron 20.0%
!!! CCTTGAATTGATTTTTTAAA+GGG - chr7_2:86069943-86069962 Msa1085390:intron 20.0%
!! ATAATAACTACACACAAAAA+AGG + chr7_2:86070129-86070148 None:intergenic 20.0%
!!! TTATTTCTTAAACCTATTTG+TGG - chr7_2:86070158-86070177 Msa1085390:intron 20.0%
!!! ACAATTATTATAGTTGGTTT+TGG - chr7_2:86070360-86070379 Msa1085390:CDS 20.0%
! TAGAAGAAAAAGAAGTGAAT+GGG + chr7_2:86069499-86069518 None:intergenic 25.0%
! AATGAAGTCATGAATGTAAA+TGG + chr7_2:86069543-86069562 None:intergenic 25.0%
! ATGCAAAGAAGGAAAAAAAA+AGG + chr7_2:86069598-86069617 None:intergenic 25.0%
! ATTTAGATTTAGTGCTTATG+TGG - chr7_2:86069694-86069713 Msa1085390:intron 25.0%
! TGAAAATATTGCTATCATCT+TGG - chr7_2:86069721-86069740 Msa1085390:intron 25.0%
!!! GGTTGAAGAATTCATTTTTA+TGG - chr7_2:86069787-86069806 Msa1085390:intron 25.0%
!! TTCTCAAAGGTTTCTAAAAA+AGG - chr7_2:86069861-86069880 Msa1085390:intron 25.0%
!!! GCCTTGAATTGATTTTTTAA+AGG - chr7_2:86069942-86069961 Msa1085390:intron 25.0%
!! CCCTTTAAAAAATCAATTCA+AGG + chr7_2:86069946-86069965 None:intergenic 25.0%
! AGATGCAACTATTTCATTAA+AGG + chr7_2:86070017-86070036 None:intergenic 25.0%
!! TAATAGTTTCACAAGAGTTT+TGG - chr7_2:86070078-86070097 Msa1085390:intron 25.0%
! TCCTCGATTAAATGAATTTA+AGG - chr7_2:86070262-86070281 Msa1085390:intron 25.0%
! ACCTTAAATTCATTTAATCG+AGG + chr7_2:86070266-86070285 None:intergenic 25.0%
! CATGCAACAATTATTATAGT+TGG - chr7_2:86070354-86070373 Msa1085390:CDS 25.0%
!!! TTCAAAGTATTTTCATGCTT+TGG - chr7_2:86070412-86070431 Msa1085390:intron 25.0%
! ACTGAGAATGTCATTAAATT+TGG + chr7_2:86070589-86070608 None:intergenic 25.0%
! TAGAAGAAAAAGAAGTGAAT+GGG + chr7_2:86069499-86069518 None:intergenic 25.0%
! AATGAAGTCATGAATGTAAA+TGG + chr7_2:86069543-86069562 None:intergenic 25.0%
! ATGCAAAGAAGGAAAAAAAA+AGG + chr7_2:86069598-86069617 None:intergenic 25.0%
! ATTTAGATTTAGTGCTTATG+TGG - chr7_2:86069694-86069713 Msa1085390:intron 25.0%
! TGAAAATATTGCTATCATCT+TGG - chr7_2:86069721-86069740 Msa1085390:intron 25.0%
!!! GGTTGAAGAATTCATTTTTA+TGG - chr7_2:86069787-86069806 Msa1085390:intron 25.0%
!! TTCTCAAAGGTTTCTAAAAA+AGG - chr7_2:86069861-86069880 Msa1085390:intron 25.0%
!!! GCCTTGAATTGATTTTTTAA+AGG - chr7_2:86069942-86069961 Msa1085390:intron 25.0%
!! CCCTTTAAAAAATCAATTCA+AGG + chr7_2:86069946-86069965 None:intergenic 25.0%
! AGATGCAACTATTTCATTAA+AGG + chr7_2:86070017-86070036 None:intergenic 25.0%
!! TAATAGTTTCACAAGAGTTT+TGG - chr7_2:86070078-86070097 Msa1085390:intron 25.0%
! TCCTCGATTAAATGAATTTA+AGG - chr7_2:86070262-86070281 Msa1085390:intron 25.0%
! ACCTTAAATTCATTTAATCG+AGG + chr7_2:86070266-86070285 None:intergenic 25.0%
! CATGCAACAATTATTATAGT+TGG - chr7_2:86070354-86070373 Msa1085390:CDS 25.0%
!!! TTCAAAGTATTTTCATGCTT+TGG - chr7_2:86070412-86070431 Msa1085390:intron 25.0%
! ACTGAGAATGTCATTAAATT+TGG + chr7_2:86070589-86070608 None:intergenic 25.0%
GTAGAAGAAAAAGAAGTGAA+TGG + chr7_2:86069500-86069519 None:intergenic 30.0%
TTTGTGATAAATGATTCTGC+AGG + chr7_2:86069634-86069653 None:intergenic 30.0%
! CATTTTTATGGAAAAGTGGT+TGG - chr7_2:86069799-86069818 Msa1085390:intron 30.0%
GCTGAATTGAAATTTCTCAA+AGG - chr7_2:86069848-86069867 Msa1085390:intron 30.0%
!! CTATCTTTATCCACTTTTCA+TGG - chr7_2:86069968-86069987 Msa1085390:intron 30.0%
ATAGAACAATCCATGAAAAG+TGG + chr7_2:86069981-86070000 None:intergenic 30.0%
!!! AGTTGTTTTAGTGTCATTGT+TGG - chr7_2:86070054-86070073 Msa1085390:intron 30.0%
!!! GTTGTTTTAGTGTCATTGTT+GGG - chr7_2:86070055-86070074 Msa1085390:intron 30.0%
! AAAAGAGTACAACCACAAAT+AGG + chr7_2:86070173-86070192 None:intergenic 30.0%
TTTGCATTCAAAATTGTCCT+TGG + chr7_2:86070327-86070346 None:intergenic 30.0%
!!! TTATTATAGTTGGTTTTGGC+TGG - chr7_2:86070364-86070383 Msa1085390:CDS 30.0%
!! TTTCATGCTTTGGAATGTAA+AGG - chr7_2:86070422-86070441 Msa1085390:intron 30.0%
TCCTTAGTTTATTACAGAGT+TGG - chr7_2:86070514-86070533 Msa1085390:intron 30.0%
TCCAACTCTGTAATAAACTA+AGG + chr7_2:86070518-86070537 None:intergenic 30.0%
GTTTATTACAGAGTTGGATA+TGG - chr7_2:86070520-86070539 Msa1085390:intron 30.0%
GTATAATTGTCTTCTGCATA+TGG - chr7_2:86070607-86070626 Msa1085390:intron 30.0%
! GATTTAGTGATTTTCCCAAA+AGG + chr7_2:86070635-86070654 None:intergenic 30.0%
GTAGAAGAAAAAGAAGTGAA+TGG + chr7_2:86069500-86069519 None:intergenic 30.0%
TTTGTGATAAATGATTCTGC+AGG + chr7_2:86069634-86069653 None:intergenic 30.0%
! CATTTTTATGGAAAAGTGGT+TGG - chr7_2:86069799-86069818 Msa1085390:intron 30.0%
GCTGAATTGAAATTTCTCAA+AGG - chr7_2:86069848-86069867 Msa1085390:intron 30.0%
!! CTATCTTTATCCACTTTTCA+TGG - chr7_2:86069968-86069987 Msa1085390:intron 30.0%
ATAGAACAATCCATGAAAAG+TGG + chr7_2:86069981-86070000 None:intergenic 30.0%
!!! AGTTGTTTTAGTGTCATTGT+TGG - chr7_2:86070054-86070073 Msa1085390:intron 30.0%
!!! GTTGTTTTAGTGTCATTGTT+GGG - chr7_2:86070055-86070074 Msa1085390:intron 30.0%
! AAAAGAGTACAACCACAAAT+AGG + chr7_2:86070173-86070192 None:intergenic 30.0%
TTTGCATTCAAAATTGTCCT+TGG + chr7_2:86070327-86070346 None:intergenic 30.0%
!!! TTATTATAGTTGGTTTTGGC+TGG - chr7_2:86070364-86070383 Msa1085390:CDS 30.0%
!! TTTCATGCTTTGGAATGTAA+AGG - chr7_2:86070422-86070441 Msa1085390:intron 30.0%
TCCTTAGTTTATTACAGAGT+TGG - chr7_2:86070514-86070533 Msa1085390:intron 30.0%
TCCAACTCTGTAATAAACTA+AGG + chr7_2:86070518-86070537 None:intergenic 30.0%
GTTTATTACAGAGTTGGATA+TGG - chr7_2:86070520-86070539 Msa1085390:intron 30.0%
GTATAATTGTCTTCTGCATA+TGG - chr7_2:86070607-86070626 Msa1085390:intron 30.0%
! GATTTAGTGATTTTCCCAAA+AGG + chr7_2:86070635-86070654 None:intergenic 30.0%
! TTGAGATTTGAGATCAAGAG+AGG + chr7_2:86069572-86069591 None:intergenic 35.0%
TTTGATCTAGCATGCAAAGA+AGG + chr7_2:86069609-86069628 None:intergenic 35.0%
!! TGCTATCATCTTGGTTTGTT+AGG - chr7_2:86069730-86069749 Msa1085390:intron 35.0%
!! ATGCATAGTTTGCTAACTTG+TGG + chr7_2:86069764-86069783 None:intergenic 35.0%
ACAAGTTAGCAAACTATGCA+TGG - chr7_2:86069763-86069782 Msa1085390:intron 35.0%
TACACCTTGAATTGAAACCA+AGG - chr7_2:86069909-86069928 Msa1085390:intron 35.0%
ACACCTTGAATTGAAACCAA+GGG - chr7_2:86069910-86069929 Msa1085390:intron 35.0%
AAACCCTTGGTTTCAATTCA+AGG + chr7_2:86069916-86069935 None:intergenic 35.0%
GATGGAAGTCCACAATTATA+TGG - chr7_2:86070229-86070248 Msa1085390:intron 35.0%
!! TAAAGGTATTGATGCTCTGT+TGG - chr7_2:86070439-86070458 Msa1085390:intron 35.0%
! TTCTGCATATGGATCCTTTT+GGG - chr7_2:86070618-86070637 Msa1085390:intron 35.0%
! TTGAGATTTGAGATCAAGAG+AGG + chr7_2:86069572-86069591 None:intergenic 35.0%
TTTGATCTAGCATGCAAAGA+AGG + chr7_2:86069609-86069628 None:intergenic 35.0%
!! TGCTATCATCTTGGTTTGTT+AGG - chr7_2:86069730-86069749 Msa1085390:intron 35.0%
!! ATGCATAGTTTGCTAACTTG+TGG + chr7_2:86069764-86069783 None:intergenic 35.0%
ACAAGTTAGCAAACTATGCA+TGG - chr7_2:86069763-86069782 Msa1085390:intron 35.0%
TACACCTTGAATTGAAACCA+AGG - chr7_2:86069909-86069928 Msa1085390:intron 35.0%
ACACCTTGAATTGAAACCAA+GGG - chr7_2:86069910-86069929 Msa1085390:intron 35.0%
AAACCCTTGGTTTCAATTCA+AGG + chr7_2:86069916-86069935 None:intergenic 35.0%
GATGGAAGTCCACAATTATA+TGG - chr7_2:86070229-86070248 Msa1085390:intron 35.0%
!! TAAAGGTATTGATGCTCTGT+TGG - chr7_2:86070439-86070458 Msa1085390:intron 35.0%
! TTCTGCATATGGATCCTTTT+GGG - chr7_2:86070618-86070637 Msa1085390:intron 35.0%
GGCAAACGAAAATGTAGAAC+AGG + chr7_2:86069522-86069541 None:intergenic 40.0%
AGTTAGCAAACTATGCATGG+CGG - chr7_2:86069766-86069785 Msa1085390:intron 40.0%
TCAAGGCAAAGTAAAACCCT+TGG + chr7_2:86069929-86069948 None:intergenic 40.0%
TGCACAAAGTCATGGTAGAA+AGG + chr7_2:86070198-86070217 None:intergenic 40.0%
TCTACCATGACTTTGTGCAA+AGG - chr7_2:86070199-86070218 Msa1085390:intron 40.0%
CATTCCTTTGCACAAAGTCA+TGG + chr7_2:86070206-86070225 None:intergenic 40.0%
TGACTTTGTGCAAAGGAATG+TGG - chr7_2:86070206-86070225 Msa1085390:intron 40.0%
GACTTTGTGCAAAGGAATGT+GGG - chr7_2:86070207-86070226 Msa1085390:intron 40.0%
CTTGCAGCACCATATAATTG+TGG + chr7_2:86070241-86070260 None:intergenic 40.0%
TTGGCTGGAATGAAAGACAA+TGG - chr7_2:86070379-86070398 Msa1085390:intron 40.0%
! ATTGATGCTCTGTTGGTGTT+TGG - chr7_2:86070446-86070465 Msa1085390:intron 40.0%
! CTTCTGCATATGGATCCTTT+TGG - chr7_2:86070617-86070636 Msa1085390:intron 40.0%
GGCAAACGAAAATGTAGAAC+AGG + chr7_2:86069522-86069541 None:intergenic 40.0%
AGTTAGCAAACTATGCATGG+CGG - chr7_2:86069766-86069785 Msa1085390:intron 40.0%
TCAAGGCAAAGTAAAACCCT+TGG + chr7_2:86069929-86069948 None:intergenic 40.0%
TGCACAAAGTCATGGTAGAA+AGG + chr7_2:86070198-86070217 None:intergenic 40.0%
TCTACCATGACTTTGTGCAA+AGG - chr7_2:86070199-86070218 Msa1085390:intron 40.0%
CATTCCTTTGCACAAAGTCA+TGG + chr7_2:86070206-86070225 None:intergenic 40.0%
TGACTTTGTGCAAAGGAATG+TGG - chr7_2:86070206-86070225 Msa1085390:intron 40.0%
GACTTTGTGCAAAGGAATGT+GGG - chr7_2:86070207-86070226 Msa1085390:intron 40.0%
CTTGCAGCACCATATAATTG+TGG + chr7_2:86070241-86070260 None:intergenic 40.0%
TTGGCTGGAATGAAAGACAA+TGG - chr7_2:86070379-86070398 Msa1085390:intron 40.0%
! ATTGATGCTCTGTTGGTGTT+TGG - chr7_2:86070446-86070465 Msa1085390:intron 40.0%
! CTTCTGCATATGGATCCTTT+TGG - chr7_2:86070617-86070636 Msa1085390:intron 40.0%
! GATTTGAGATCAAGAGAGGC+TGG + chr7_2:86069568-86069587 None:intergenic 45.0%
TATGGAAAAGTGGTTGGAGC+TGG - chr7_2:86069805-86069824 Msa1085390:intron 45.0%
TTGTGCAAAGGAATGTGGGA+TGG - chr7_2:86070211-86070230 Msa1085390:intron 45.0%
! GATTTGAGATCAAGAGAGGC+TGG + chr7_2:86069568-86069587 None:intergenic 45.0%
TATGGAAAAGTGGTTGGAGC+TGG - chr7_2:86069805-86069824 Msa1085390:intron 45.0%
TTGTGCAAAGGAATGTGGGA+TGG - chr7_2:86070211-86070230 Msa1085390:intron 45.0%
GATTCATCGTCGTGAGACCA+AGG - chr7_2:86070307-86070326 Msa1085390:intron 50.0%
GGGGAGGATGCTTGAATAAG+AGG + chr7_2:86070657-86070676 None:intergenic 50.0%
GATTCATCGTCGTGAGACCA+AGG - chr7_2:86070307-86070326 Msa1085390:intron 50.0%
GGGGAGGATGCTTGAATAAG+AGG + chr7_2:86070657-86070676 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 86069457 86070683 86069457 ID=Msa1085390;Name=Msa1085390
chr7_2 mRNA 86069457 86070683 86069457 ID=Msa1085390-mRNA-1;Parent=Msa1085390;Name=Msa1085390-mRNA-1;_AED=0.56;_eAED=0.77;_QI=0|0|0|1|0.5|0.66|3|0|77
chr7_2 exon 86070678 86070683 86070678 ID=Msa1085390-mRNA-1:exon:23282;Parent=Msa1085390-mRNA-1
chr7_2 exon 86070314 86070388 86070314 ID=Msa1085390-mRNA-1:exon:23281;Parent=Msa1085390-mRNA-1
chr7_2 exon 86069457 86069609 86069457 ID=Msa1085390-mRNA-1:exon:23280;Parent=Msa1085390-mRNA-1
chr7_2 CDS 86070678 86070683 86070678 ID=Msa1085390-mRNA-1:cds;Parent=Msa1085390-mRNA-1
chr7_2 CDS 86070314 86070388 86070314 ID=Msa1085390-mRNA-1:cds;Parent=Msa1085390-mRNA-1
chr7_2 CDS 86069457 86069609 86069457 ID=Msa1085390-mRNA-1:cds;Parent=Msa1085390-mRNA-1
Gene Sequence

>Msa1085390

ATGAGTGTTGTTGAGCCACAAGTTAGCAAACTATGCATGGCGGTTGAAGAATTCATTTTTATGGAAAAGTGGTTGGAGCTGAGTTGGATATGGAACAATATAATAGTAGCAATATCTCATTTTGTATTTGCATACACCAAATTTAATGACATTCTCAGTATAATTGTCTTCTGCATATGGATCCTTTTGGGAAAATCACTAAATCCTCTTATTCAAGCATCCTCCCCTTTCTAA

Protein sequence

>Msa1085390

MSVVEPQVSKLCMAVEEFIFMEKWLELSWIWNNIIVAISHFVFAYTKFNDILSIIVFCIWILLGKSLNPLIQASSPF*