Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0880340 | 0.831424 | 1.682602e-55 | -8.615850e-47 |
| Msa0029810 | Msa0880340 | 0.963666 | 2.295254e-122 | -8.615850e-47 |
| Msa0661870 | Msa0880340 | 0.800506 | 1.395894e-48 | -8.615850e-47 |
| Msa0157290 | Msa0880340 | 0.831046 | 2.084188e-55 | -8.615850e-47 |
| Msa0437990 | Msa0880340 | 0.804927 | 1.707781e-49 | -8.615850e-47 |
| Msa0243100 | Msa0880340 | 0.813526 | 2.448343e-51 | -8.615850e-47 |
| Msa0512900 | Msa0880340 | 0.805142 | 1.539626e-49 | -8.615850e-47 |
| Msa0880140 | Msa0880340 | 0.829064 | 6.342488e-55 | -8.615850e-47 |
| Msa0880340 | Msa0922720 | 0.808123 | 3.615249e-50 | -8.615850e-47 |
| Msa0880340 | Msa1014540 | 0.811390 | 7.170988e-51 | -8.615850e-47 |
| Msa0880340 | Msa1024240 | 0.801636 | 8.200172e-49 | -8.615850e-47 |
| Msa0880340 | Msa1085390 | 0.947364 | 7.764618e-106 | -8.615850e-47 |
| Msa0880340 | Msa1438250 | 0.816638 | 4.987054e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 132 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGAGAATGTCATTAAATT+TGG | 0.171054 | 6_1:+26864106 | None:intergenic |
| CTTCTGCATATGGATCCTTT+TGG | 0.279760 | 6_1:-26864075 | Msa0880340:CDS |
| GTTTATTACAGAGTTGGATA+TGG | 0.319512 | 6_1:-26864172 | Msa0880340:intron |
| GGGGAGGATGCTTGAATAAG+AGG | 0.376419 | 6_1:+26864038 | None:intergenic |
| GTATAATTGTCTTCTGCATA+TGG | 0.387682 | 6_1:-26864085 | Msa0880340:CDS |
| TATGGAAAAGTGGTTGGAGC+TGG | 0.462385 | 6_1:-26864892 | Msa0880340:intron |
| TTTAGTGTATAGTTAGAAAG+GGG | 0.507137 | 6_1:+26864019 | None:intergenic |
| ATGCATAGTTTGCTAACTTG+TGG | 0.540764 | 6_1:+26864936 | None:intergenic |
| AGTGTATAGTTAGAAAGGGG+AGG | 0.548704 | 6_1:+26864022 | None:intergenic |
| ACAAGTTAGCAAACTATGCA+TGG | 0.605098 | 6_1:-26864934 | Msa0880340:CDS |
| AGTTAGCAAACTATGCATGG+CGG | 0.782698 | 6_1:-26864931 | Msa0880340:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTGAATTGATTTTTTTAAA+GGG | - | chr6_1:26864518-26864537 | Msa0880340:intron | 15.0% |
| !!! | CTTGAATTGATTTTTTTAAA+GGG | - | chr6_1:26864518-26864537 | Msa0880340:intron | 15.0% |
| !!! | AATTCATTTTTATGGAAAAG+TGG | - | chr6_1:26864369-26864388 | Msa0880340:intron | 20.0% |
| !!! | CCTTTAAAAAAATCAATTCA+AGG | + | chr6_1:26864520-26864539 | None:intergenic | 20.0% |
| !!! | CCTTGAATTGATTTTTTTAA+AGG | - | chr6_1:26864517-26864536 | Msa0880340:intron | 20.0% |
| !! | ATAATAACTACACACAAAAA+AGG | + | chr6_1:26864708-26864727 | None:intergenic | 20.0% |
| !!! | TTATTTCTTAAACCTATTTG+TGG | - | chr6_1:26864737-26864756 | Msa0880340:intron | 20.0% |
| !!! | ACAATTATTATAGTTGGTTT+TGG | - | chr6_1:26864939-26864958 | Msa0880340:CDS | 20.0% |
| !!! | AATTCATTTTTATGGAAAAG+TGG | - | chr6_1:26864369-26864388 | Msa0880340:intron | 20.0% |
| !!! | CCTTTAAAAAAATCAATTCA+AGG | + | chr6_1:26864520-26864539 | None:intergenic | 20.0% |
| !!! | CCTTGAATTGATTTTTTTAA+AGG | - | chr6_1:26864517-26864536 | Msa0880340:intron | 20.0% |
| !! | ATAATAACTACACACAAAAA+AGG | + | chr6_1:26864708-26864727 | None:intergenic | 20.0% |
| !!! | TTATTTCTTAAACCTATTTG+TGG | - | chr6_1:26864737-26864756 | Msa0880340:intron | 20.0% |
| !!! | ACAATTATTATAGTTGGTTT+TGG | - | chr6_1:26864939-26864958 | Msa0880340:CDS | 20.0% |
| ! | TAGAAGAAAAAGAAGTGAAT+GGG | + | chr6_1:26864073-26864092 | None:intergenic | 25.0% |
| ! | AATGAAGTCATGAATGTAAA+TGG | + | chr6_1:26864117-26864136 | None:intergenic | 25.0% |
| ! | ATGCAAAGAAGGAAAAAAAA+AGG | + | chr6_1:26864172-26864191 | None:intergenic | 25.0% |
| ! | ATTTAGATTTAGTGCTTATG+TGG | - | chr6_1:26864268-26864287 | Msa0880340:intron | 25.0% |
| ! | TGAAAATATTGCTATCATCT+TGG | - | chr6_1:26864295-26864314 | Msa0880340:intron | 25.0% |
| !!! | GGTTGAAGAATTCATTTTTA+TGG | - | chr6_1:26864361-26864380 | Msa0880340:intron | 25.0% |
| !! | TTCTCAAAGGTTTCTAAAAA+AGG | - | chr6_1:26864435-26864454 | Msa0880340:intron | 25.0% |
| !!! | TATCTTTAATCCACTTTTCA+TGG | - | chr6_1:26864544-26864563 | Msa0880340:intron | 25.0% |
| !! | TAATAGTTTCACAAGAGTTT+TGG | - | chr6_1:26864657-26864676 | Msa0880340:intron | 25.0% |
| ! | TCCTCGATTAAATGAATTTA+AGG | - | chr6_1:26864841-26864860 | Msa0880340:intron | 25.0% |
| ! | ACCTTAAATTCATTTAATCG+AGG | + | chr6_1:26864845-26864864 | None:intergenic | 25.0% |
| ! | CATGCAACAATTATTATAGT+TGG | - | chr6_1:26864933-26864952 | Msa0880340:CDS | 25.0% |
| !!! | TTCAAAGTATTTTCATGCTT+TGG | - | chr6_1:26864991-26865010 | Msa0880340:intron | 25.0% |
| ! | ACTGAGAATGTCATTAAATT+TGG | + | chr6_1:26865168-26865187 | None:intergenic | 25.0% |
| ! | TAGAAGAAAAAGAAGTGAAT+GGG | + | chr6_1:26864073-26864092 | None:intergenic | 25.0% |
| ! | AATGAAGTCATGAATGTAAA+TGG | + | chr6_1:26864117-26864136 | None:intergenic | 25.0% |
| ! | ATGCAAAGAAGGAAAAAAAA+AGG | + | chr6_1:26864172-26864191 | None:intergenic | 25.0% |
| ! | ATTTAGATTTAGTGCTTATG+TGG | - | chr6_1:26864268-26864287 | Msa0880340:intron | 25.0% |
| ! | TGAAAATATTGCTATCATCT+TGG | - | chr6_1:26864295-26864314 | Msa0880340:intron | 25.0% |
| !!! | GGTTGAAGAATTCATTTTTA+TGG | - | chr6_1:26864361-26864380 | Msa0880340:intron | 25.0% |
| !! | TTCTCAAAGGTTTCTAAAAA+AGG | - | chr6_1:26864435-26864454 | Msa0880340:intron | 25.0% |
| !!! | TATCTTTAATCCACTTTTCA+TGG | - | chr6_1:26864544-26864563 | Msa0880340:intron | 25.0% |
| !! | TAATAGTTTCACAAGAGTTT+TGG | - | chr6_1:26864657-26864676 | Msa0880340:intron | 25.0% |
| ! | TCCTCGATTAAATGAATTTA+AGG | - | chr6_1:26864841-26864860 | Msa0880340:intron | 25.0% |
| ! | ACCTTAAATTCATTTAATCG+AGG | + | chr6_1:26864845-26864864 | None:intergenic | 25.0% |
| ! | CATGCAACAATTATTATAGT+TGG | - | chr6_1:26864933-26864952 | Msa0880340:CDS | 25.0% |
| !!! | TTCAAAGTATTTTCATGCTT+TGG | - | chr6_1:26864991-26865010 | Msa0880340:intron | 25.0% |
| ! | ACTGAGAATGTCATTAAATT+TGG | + | chr6_1:26865168-26865187 | None:intergenic | 25.0% |
| GTAGAAGAAAAAGAAGTGAA+TGG | + | chr6_1:26864074-26864093 | None:intergenic | 30.0% | |
| TTTGTGATAAATGATTCTGC+AGG | + | chr6_1:26864208-26864227 | None:intergenic | 30.0% | |
| ! | CATTTTTATGGAAAAGTGGT+TGG | - | chr6_1:26864373-26864392 | Msa0880340:intron | 30.0% |
| GCTGAATTGAAATTTCTCAA+AGG | - | chr6_1:26864422-26864441 | Msa0880340:intron | 30.0% | |
| ATAGAACAATCCATGAAAAG+TGG | + | chr6_1:26864557-26864576 | None:intergenic | 30.0% | |
| AGATGCGACTATTTCATTAA+AGG | + | chr6_1:26864596-26864615 | None:intergenic | 30.0% | |
| !!! | AGTTGTTTTAGTGTCATTGT+TGG | - | chr6_1:26864633-26864652 | Msa0880340:intron | 30.0% |
| !!! | GTTGTTTTAGTGTCATTGTT+GGG | - | chr6_1:26864634-26864653 | Msa0880340:intron | 30.0% |
| ! | AAAAGAGTACAACCACAAAT+AGG | + | chr6_1:26864752-26864771 | None:intergenic | 30.0% |
| TTTGCATTCAAAATTGTCCT+TGG | + | chr6_1:26864906-26864925 | None:intergenic | 30.0% | |
| !!! | TTATTATAGTTGGTTTTGGC+TGG | - | chr6_1:26864943-26864962 | Msa0880340:CDS | 30.0% |
| !! | TTTCATGCTTTGGAATGTAA+AGG | - | chr6_1:26865001-26865020 | Msa0880340:intron | 30.0% |
| TCCTTAGTTTATTACAGAGT+TGG | - | chr6_1:26865093-26865112 | Msa0880340:intron | 30.0% | |
| TCCAACTCTGTAATAAACTA+AGG | + | chr6_1:26865097-26865116 | None:intergenic | 30.0% | |
| GTTTATTACAGAGTTGGATA+TGG | - | chr6_1:26865099-26865118 | Msa0880340:intron | 30.0% | |
| GTATAATTGTCTTCTGCATA+TGG | - | chr6_1:26865186-26865205 | Msa0880340:intron | 30.0% | |
| ! | GATTTAGTGATTTTCCCAAA+AGG | + | chr6_1:26865214-26865233 | None:intergenic | 30.0% |
| GTAGAAGAAAAAGAAGTGAA+TGG | + | chr6_1:26864074-26864093 | None:intergenic | 30.0% | |
| TTTGTGATAAATGATTCTGC+AGG | + | chr6_1:26864208-26864227 | None:intergenic | 30.0% | |
| ! | CATTTTTATGGAAAAGTGGT+TGG | - | chr6_1:26864373-26864392 | Msa0880340:intron | 30.0% |
| GCTGAATTGAAATTTCTCAA+AGG | - | chr6_1:26864422-26864441 | Msa0880340:intron | 30.0% | |
| ATAGAACAATCCATGAAAAG+TGG | + | chr6_1:26864557-26864576 | None:intergenic | 30.0% | |
| AGATGCGACTATTTCATTAA+AGG | + | chr6_1:26864596-26864615 | None:intergenic | 30.0% | |
| !!! | AGTTGTTTTAGTGTCATTGT+TGG | - | chr6_1:26864633-26864652 | Msa0880340:intron | 30.0% |
| !!! | GTTGTTTTAGTGTCATTGTT+GGG | - | chr6_1:26864634-26864653 | Msa0880340:intron | 30.0% |
| ! | AAAAGAGTACAACCACAAAT+AGG | + | chr6_1:26864752-26864771 | None:intergenic | 30.0% |
| TTTGCATTCAAAATTGTCCT+TGG | + | chr6_1:26864906-26864925 | None:intergenic | 30.0% | |
| !!! | TTATTATAGTTGGTTTTGGC+TGG | - | chr6_1:26864943-26864962 | Msa0880340:CDS | 30.0% |
| !! | TTTCATGCTTTGGAATGTAA+AGG | - | chr6_1:26865001-26865020 | Msa0880340:intron | 30.0% |
| TCCTTAGTTTATTACAGAGT+TGG | - | chr6_1:26865093-26865112 | Msa0880340:intron | 30.0% | |
| TCCAACTCTGTAATAAACTA+AGG | + | chr6_1:26865097-26865116 | None:intergenic | 30.0% | |
| GTTTATTACAGAGTTGGATA+TGG | - | chr6_1:26865099-26865118 | Msa0880340:intron | 30.0% | |
| GTATAATTGTCTTCTGCATA+TGG | - | chr6_1:26865186-26865205 | Msa0880340:intron | 30.0% | |
| ! | GATTTAGTGATTTTCCCAAA+AGG | + | chr6_1:26865214-26865233 | None:intergenic | 30.0% |
| ! | TTGAGATTTGAGATCAAGAG+AGG | + | chr6_1:26864146-26864165 | None:intergenic | 35.0% |
| TTTGATCTAGCATGCAAAGA+AGG | + | chr6_1:26864183-26864202 | None:intergenic | 35.0% | |
| !! | TGCTATCATCTTGGTTTGTT+AGG | - | chr6_1:26864304-26864323 | Msa0880340:intron | 35.0% |
| !! | ATGCATAGTTTGCTAACTTG+TGG | + | chr6_1:26864338-26864357 | None:intergenic | 35.0% |
| ACAAGTTAGCAAACTATGCA+TGG | - | chr6_1:26864337-26864356 | Msa0880340:intron | 35.0% | |
| TACACCTTGAATTGAAACCA+AGG | - | chr6_1:26864483-26864502 | Msa0880340:intron | 35.0% | |
| ACACCTTGAATTGAAACCAA+GGG | - | chr6_1:26864484-26864503 | Msa0880340:intron | 35.0% | |
| AAACCCTTGGTTTCAATTCA+AGG | + | chr6_1:26864490-26864509 | None:intergenic | 35.0% | |
| GATGGAAGTCCACAATTATA+TGG | - | chr6_1:26864808-26864827 | Msa0880340:intron | 35.0% | |
| !! | TAAAGGTATTGATGCTCTGT+TGG | - | chr6_1:26865018-26865037 | Msa0880340:intron | 35.0% |
| ! | TTCTGCATATGGATCCTTTT+GGG | - | chr6_1:26865197-26865216 | Msa0880340:intron | 35.0% |
| ! | TTGAGATTTGAGATCAAGAG+AGG | + | chr6_1:26864146-26864165 | None:intergenic | 35.0% |
| TTTGATCTAGCATGCAAAGA+AGG | + | chr6_1:26864183-26864202 | None:intergenic | 35.0% | |
| !! | TGCTATCATCTTGGTTTGTT+AGG | - | chr6_1:26864304-26864323 | Msa0880340:intron | 35.0% |
| !! | ATGCATAGTTTGCTAACTTG+TGG | + | chr6_1:26864338-26864357 | None:intergenic | 35.0% |
| ACAAGTTAGCAAACTATGCA+TGG | - | chr6_1:26864337-26864356 | Msa0880340:intron | 35.0% | |
| TACACCTTGAATTGAAACCA+AGG | - | chr6_1:26864483-26864502 | Msa0880340:intron | 35.0% | |
| ACACCTTGAATTGAAACCAA+GGG | - | chr6_1:26864484-26864503 | Msa0880340:intron | 35.0% | |
| AAACCCTTGGTTTCAATTCA+AGG | + | chr6_1:26864490-26864509 | None:intergenic | 35.0% | |
| GATGGAAGTCCACAATTATA+TGG | - | chr6_1:26864808-26864827 | Msa0880340:intron | 35.0% | |
| !! | TAAAGGTATTGATGCTCTGT+TGG | - | chr6_1:26865018-26865037 | Msa0880340:intron | 35.0% |
| ! | TTCTGCATATGGATCCTTTT+GGG | - | chr6_1:26865197-26865216 | Msa0880340:intron | 35.0% |
| GGCAAACGAAAATGTAGAAC+AGG | + | chr6_1:26864096-26864115 | None:intergenic | 40.0% | |
| AGTTAGCAAACTATGCATGG+CGG | - | chr6_1:26864340-26864359 | Msa0880340:intron | 40.0% | |
| TCAAGGCAAAGTAAAACCCT+TGG | + | chr6_1:26864503-26864522 | None:intergenic | 40.0% | |
| TGCACAAAGTCATGGTAGAA+AGG | + | chr6_1:26864777-26864796 | None:intergenic | 40.0% | |
| TCTACCATGACTTTGTGCAA+AGG | - | chr6_1:26864778-26864797 | Msa0880340:intron | 40.0% | |
| CATTCCTTTGCACAAAGTCA+TGG | + | chr6_1:26864785-26864804 | None:intergenic | 40.0% | |
| TGACTTTGTGCAAAGGAATG+TGG | - | chr6_1:26864785-26864804 | Msa0880340:intron | 40.0% | |
| GACTTTGTGCAAAGGAATGT+GGG | - | chr6_1:26864786-26864805 | Msa0880340:intron | 40.0% | |
| CTTGCAGCACCATATAATTG+TGG | + | chr6_1:26864820-26864839 | None:intergenic | 40.0% | |
| TTGGCTGGAATGAAAGACAA+TGG | - | chr6_1:26864958-26864977 | Msa0880340:intron | 40.0% | |
| ! | ATTGATGCTCTGTTGGTGTT+TGG | - | chr6_1:26865025-26865044 | Msa0880340:intron | 40.0% |
| ! | CTTCTGCATATGGATCCTTT+TGG | - | chr6_1:26865196-26865215 | Msa0880340:intron | 40.0% |
| GGCAAACGAAAATGTAGAAC+AGG | + | chr6_1:26864096-26864115 | None:intergenic | 40.0% | |
| AGTTAGCAAACTATGCATGG+CGG | - | chr6_1:26864340-26864359 | Msa0880340:intron | 40.0% | |
| TCAAGGCAAAGTAAAACCCT+TGG | + | chr6_1:26864503-26864522 | None:intergenic | 40.0% | |
| TGCACAAAGTCATGGTAGAA+AGG | + | chr6_1:26864777-26864796 | None:intergenic | 40.0% | |
| TCTACCATGACTTTGTGCAA+AGG | - | chr6_1:26864778-26864797 | Msa0880340:intron | 40.0% | |
| CATTCCTTTGCACAAAGTCA+TGG | + | chr6_1:26864785-26864804 | None:intergenic | 40.0% | |
| TGACTTTGTGCAAAGGAATG+TGG | - | chr6_1:26864785-26864804 | Msa0880340:intron | 40.0% | |
| GACTTTGTGCAAAGGAATGT+GGG | - | chr6_1:26864786-26864805 | Msa0880340:intron | 40.0% | |
| CTTGCAGCACCATATAATTG+TGG | + | chr6_1:26864820-26864839 | None:intergenic | 40.0% | |
| TTGGCTGGAATGAAAGACAA+TGG | - | chr6_1:26864958-26864977 | Msa0880340:intron | 40.0% | |
| ! | ATTGATGCTCTGTTGGTGTT+TGG | - | chr6_1:26865025-26865044 | Msa0880340:intron | 40.0% |
| ! | CTTCTGCATATGGATCCTTT+TGG | - | chr6_1:26865196-26865215 | Msa0880340:intron | 40.0% |
| ! | GATTTGAGATCAAGAGAGGC+TGG | + | chr6_1:26864142-26864161 | None:intergenic | 45.0% |
| TATGGAAAAGTGGTTGGAGC+TGG | - | chr6_1:26864379-26864398 | Msa0880340:intron | 45.0% | |
| TTGTGCAAAGGAATGTGGGA+TGG | - | chr6_1:26864790-26864809 | Msa0880340:intron | 45.0% | |
| ! | GATTTGAGATCAAGAGAGGC+TGG | + | chr6_1:26864142-26864161 | None:intergenic | 45.0% |
| TATGGAAAAGTGGTTGGAGC+TGG | - | chr6_1:26864379-26864398 | Msa0880340:intron | 45.0% | |
| TTGTGCAAAGGAATGTGGGA+TGG | - | chr6_1:26864790-26864809 | Msa0880340:intron | 45.0% | |
| GATTCATCGTCGTGAGACCA+AGG | - | chr6_1:26864886-26864905 | Msa0880340:intron | 50.0% | |
| GGGGAGGATGCTTGAATAAG+AGG | + | chr6_1:26865236-26865255 | None:intergenic | 50.0% | |
| GATTCATCGTCGTGAGACCA+AGG | - | chr6_1:26864886-26864905 | Msa0880340:intron | 50.0% | |
| GGGGAGGATGCTTGAATAAG+AGG | + | chr6_1:26865236-26865255 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 26864031 | 26865262 | 26864031 | ID=Msa0880340;Name=Msa0880340 |
| chr6_1 | mRNA | 26864031 | 26865262 | 26864031 | ID=Msa0880340-mRNA-1;Parent=Msa0880340;Name=Msa0880340-mRNA-1;_AED=0.56;_eAED=0.77;_QI=0|0|0|1|0|0|3|0|77 |
| chr6_1 | exon | 26865257 | 26865262 | 26865257 | ID=Msa0880340-mRNA-1:exon:6572;Parent=Msa0880340-mRNA-1 |
| chr6_1 | exon | 26864893 | 26864967 | 26864893 | ID=Msa0880340-mRNA-1:exon:6571;Parent=Msa0880340-mRNA-1 |
| chr6_1 | exon | 26864031 | 26864183 | 26864031 | ID=Msa0880340-mRNA-1:exon:6570;Parent=Msa0880340-mRNA-1 |
| chr6_1 | CDS | 26865257 | 26865262 | 26865257 | ID=Msa0880340-mRNA-1:cds;Parent=Msa0880340-mRNA-1 |
| chr6_1 | CDS | 26864893 | 26864967 | 26864893 | ID=Msa0880340-mRNA-1:cds;Parent=Msa0880340-mRNA-1 |
| chr6_1 | CDS | 26864031 | 26864183 | 26864031 | ID=Msa0880340-mRNA-1:cds;Parent=Msa0880340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
