AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0437990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCAGGTCAAGGCTGATTT+TGG 0.138857 3_3:-2007001 Msa0437990:intron
AACCCGCATTTCTAACTTAA+TGG 0.155002 3_3:+2006927 None:intergenic
TACTGGTGATGTTTCTAAAA+AGG 0.173626 3_3:-2007226 Msa0437990:five_prime_UTR
TCGGATTAACCAACACAATT+AGG 0.275265 3_3:-2007292 Msa0437990:five_prime_UTR
TGAGTTGAGAAGGTTGAATA+AGG 0.323601 3_3:-2007493 Msa0437990:five_prime_UTR
CACTTGTAGGCTCTCTTCTA+TGG 0.338739 3_3:-2007445 Msa0437990:five_prime_UTR
ATTTCTAACTTAATGGTACT+TGG 0.426986 3_3:+2006934 None:intergenic
TTAACTTAAATCATACTGAT+AGG 0.430639 3_3:-2007117 Msa0437990:CDS
AACCAACACAATTAGGCTCT+TGG 0.438251 3_3:-2007285 Msa0437990:five_prime_UTR
GATAAGAGGAAGCGGTGAAG+GGG 0.453973 3_3:-2007560 None:intergenic
TACCATTAAGTTAGAAATGC+GGG 0.455867 3_3:-2006929 Msa0437990:exon
TTGTAATAGAAGATATGAAT+AGG 0.461497 3_3:-2007321 Msa0437990:five_prime_UTR
AGCCTTGCTCTGCAAATACT+TGG 0.488191 3_3:+2006958 None:intergenic
AGATAAGAGGAAGCGGTGAA+GGG 0.491215 3_3:-2007561 None:intergenic
AGATATGAATAGGCTCTCTT+CGG 0.510356 3_3:-2007311 Msa0437990:five_prime_UTR
AGAAGAGGAACCTCACTTGT+AGG 0.517175 3_3:-2007458 Msa0437990:five_prime_UTR
GTCCAAGTATTTGCAGAGCA+AGG 0.527011 3_3:-2006960 Msa0437990:CDS
AGCCAAGAGCCTAATTGTGT+TGG 0.531992 3_3:+2007283 None:intergenic
TTACTGAGTTGGACTTAAAG+TGG 0.536061 3_3:-2007173 Msa0437990:five_prime_UTR
ATAAGAGGAAGCGGTGAAGG+GGG 0.538898 3_3:-2007559 None:intergenic
TTGAAGTTGTTGAGTTGAGA+AGG 0.559117 3_3:-2007503 Msa0437990:five_prime_UTR
TGTATGCTATCTTACTGAGT+TGG 0.580470 3_3:-2007184 Msa0437990:five_prime_UTR
AGGAGCTGCATATGTAGAAG+AGG 0.591272 3_3:-2007473 Msa0437990:five_prime_UTR
ATGATTTAAGTTAATAACCA+TGG 0.599391 3_3:+2007126 None:intergenic
GTACCATTAAGTTAGAAATG+CGG 0.611734 3_3:-2006930 Msa0437990:exon
TCATACTGATAGGACAGTGA+TGG 0.631868 3_3:-2007107 Msa0437990:intron
TAGAAGAGAGCCTACAAGTG+AGG 0.633933 3_3:+2007448 None:intergenic
TAGTTGTGCCTGCCAAAACA+CGG 0.641537 3_3:+2007247 None:intergenic
TAACTTGCTGAGAAATACCA+TGG 0.684621 3_3:-2007143 Msa0437990:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAATAGAAGATATGAAT+AGG - chr3_3:2007094-2007113 Msa0437990:intron 20.0%
!!! ATGATTTAAGTTAATAACCA+TGG + chr3_3:2007292-2007311 None:intergenic 20.0%
!! TTAACTTAAATCATACTGAT+AGG - chr3_3:2007298-2007317 Msa0437990:five_prime_UTR 20.0%
!! TGACATAATTTCTTTTCTCA+TGG - chr3_3:2007062-2007081 Msa0437990:intron 25.0%
!! GACATAATTTCTTTTCTCAT+GGG - chr3_3:2007063-2007082 Msa0437990:intron 25.0%
! ATTTCTAACTTAATGGTACT+TGG + chr3_3:2007484-2007503 None:intergenic 25.0%
! TACTGGTGATGTTTCTAAAA+AGG - chr3_3:2007189-2007208 Msa0437990:five_prime_UTR 30.0%
! ACTGGTGATGTTTCTAAAAA+GGG - chr3_3:2007190-2007209 Msa0437990:five_prime_UTR 30.0%
!! AGTACTTGATAACCTCATTT+TGG + chr3_3:2007367-2007386 None:intergenic 30.0%
GTACCATTAAGTTAGAAATG+CGG - chr3_3:2007485-2007504 Msa0437990:five_prime_UTR 30.0%
TACCATTAAGTTAGAAATGC+GGG - chr3_3:2007486-2007505 Msa0437990:five_prime_UTR 30.0%
TAAAAAGTAAACAAGCAGCA+AGG + chr3_3:2007526-2007545 None:intergenic 30.0%
!!! TTGCTGCTTGTTTACTTTTT+AGG - chr3_3:2007525-2007544 Msa0437990:five_prime_UTR 30.0%
!! TTGAAGTTGTTGAGTTGAGA+AGG - chr3_3:2006912-2006931 Msa0437990:three_prime_UTR 35.0%
! TGAGTTGAGAAGGTTGAATA+AGG - chr3_3:2006922-2006941 Msa0437990:three_prime_UTR 35.0%
AGATATGAATAGGCTCTCTT+CGG - chr3_3:2007104-2007123 Msa0437990:intron 35.0%
TCGGATTAACCAACACAATT+AGG - chr3_3:2007123-2007142 Msa0437990:CDS 35.0%
!! TTTTGATTGATGCCGTGTTT+TGG - chr3_3:2007156-2007175 Msa0437990:five_prime_UTR 35.0%
! ATGTTTCTAAAAAGGGTCGA+TGG - chr3_3:2007197-2007216 Msa0437990:five_prime_UTR 35.0%
TGTATGCTATCTTACTGAGT+TGG - chr3_3:2007231-2007250 Msa0437990:five_prime_UTR 35.0%
! TTACTGAGTTGGACTTAAAG+TGG - chr3_3:2007242-2007261 Msa0437990:five_prime_UTR 35.0%
!! TAACTTGCTGAGAAATACCA+TGG - chr3_3:2007272-2007291 Msa0437990:five_prime_UTR 35.0%
TTTGGACAGAGTAAACTTCA+AGG + chr3_3:2007349-2007368 None:intergenic 35.0%
AGTTTACTCTGTCCAAAATG+AGG - chr3_3:2007352-2007371 Msa0437990:five_prime_UTR 35.0%
AACCCGCATTTCTAACTTAA+TGG + chr3_3:2007491-2007510 None:intergenic 35.0%
AACCAACACAATTAGGCTCT+TGG - chr3_3:2007130-2007149 Msa0437990:exon 40.0%
TCATACTGATAGGACAGTGA+TGG - chr3_3:2007308-2007327 Msa0437990:five_prime_UTR 40.0%
CAGTAGAGGATAGGCAAATT+GGG + chr3_3:2007397-2007416 None:intergenic 40.0%
AGGAGCTGCATATGTAGAAG+AGG - chr3_3:2006942-2006961 Msa0437990:exon 45.0%
AGAAGAGGAACCTCACTTGT+AGG - chr3_3:2006957-2006976 Msa0437990:CDS 45.0%
TAGAAGAGAGCCTACAAGTG+AGG + chr3_3:2006970-2006989 None:intergenic 45.0%
CACTTGTAGGCTCTCTTCTA+TGG - chr3_3:2006970-2006989 Msa0437990:CDS 45.0%
AGCCAAGAGCCTAATTGTGT+TGG + chr3_3:2007135-2007154 None:intergenic 45.0%
TAGTTGTGCCTGCCAAAACA+CGG + chr3_3:2007171-2007190 None:intergenic 45.0%
GCAGTAGAGGATAGGCAAAT+TGG + chr3_3:2007398-2007417 None:intergenic 45.0%
ATTTGCCTATCCTCTACTGC+AGG - chr3_3:2007398-2007417 Msa0437990:five_prime_UTR 45.0%
!! TGCAGGTCAAGGCTGATTTT+GGG - chr3_3:2007415-2007434 Msa0437990:five_prime_UTR 45.0%
GTCCAAGTATTTGCAGAGCA+AGG - chr3_3:2007455-2007474 Msa0437990:five_prime_UTR 45.0%
AGCCTTGCTCTGCAAATACT+TGG + chr3_3:2007460-2007479 None:intergenic 45.0%
!!! GATTGATGCCGTGTTTTGGC+AGG - chr3_3:2007160-2007179 Msa0437990:five_prime_UTR 50.0%
!! GTTTTGGCAGGCACAACTAC+TGG - chr3_3:2007172-2007191 Msa0437990:five_prime_UTR 50.0%
CTTGACCTGCAGTAGAGGAT+AGG + chr3_3:2007406-2007425 None:intergenic 50.0%
CTATCCTCTACTGCAGGTCA+AGG - chr3_3:2007404-2007423 Msa0437990:five_prime_UTR 50.0%
!! CTGCAGGTCAAGGCTGATTT+TGG - chr3_3:2007414-2007433 Msa0437990:five_prime_UTR 50.0%
TCAGCCTTGACCTGCAGTAG+AGG + chr3_3:2007411-2007430 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 2006869 2007568 2006869 ID=Msa0437990;Name=Msa0437990
chr3_3 mRNA 2006869 2007568 2006869 ID=Msa0437990-mRNA-1;Parent=Msa0437990;Name=Msa0437990-mRNA-1;_AED=0.28;_eAED=0.28;_QI=422|1|1|1|1|1|2|74|37
chr3_3 exon 2006869 2007017 2006869 ID=Msa0437990-mRNA-1:exon:466;Parent=Msa0437990-mRNA-1
chr3_3 exon 2007108 2007568 2007108 ID=Msa0437990-mRNA-1:exon:465;Parent=Msa0437990-mRNA-1
chr3_3 five_prime_UTR 2007147 2007568 2007147 ID=Msa0437990-mRNA-1:five_prime_utr;Parent=Msa0437990-mRNA-1
chr3_3 CDS 2007108 2007146 2007108 ID=Msa0437990-mRNA-1:cds;Parent=Msa0437990-mRNA-1
chr3_3 CDS 2006943 2007017 2006943 ID=Msa0437990-mRNA-1:cds;Parent=Msa0437990-mRNA-1
chr3_3 three_prime_UTR 2006869 2006942 2006869 ID=Msa0437990-mRNA-1:three_prime_utr;Parent=Msa0437990-mRNA-1
Gene Sequence

>Msa0437990

ATGGTTATTAACTTAAATCATACTGATAGGACAGTGATGGTCAAGGCTGATTTTGGGTCTGTTATTGATGCTTTGTCCAAGTATTTGCAGAGCAAGGCTGCCAAGTACCATTAA

Protein sequence

>Msa0437990

MVINLNHTDRTVMVKADFGSVIDALSKYLQSKAAKYH*