AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0041280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041280 MtrunA17_Chr1g0206651 96.032 252 10 0 1 252 1 252 0.0 503
Msa0041280 MtrunA17_Chr1g0206601 71.484 256 67 4 1 251 22 276 1.21e-132 375
Msa0041280 MtrunA17_Chr1g0206581 67.170 265 71 6 1 251 18 280 1.02e-123 353
Msa0041280 MtrunA17_Chr1g0154341 55.242 248 104 4 10 251 7 253 4.79e-92 273
Msa0041280 MtrunA17_Chr1g0154361 55.102 245 103 3 12 250 8 251 5.50e-91 271
Msa0041280 MtrunA17_Chr1g0154321 54.183 251 103 6 12 251 8 257 7.82e-85 268
Msa0041280 MtrunA17_Chr1g0206571 54.545 231 103 2 23 252 30 259 4.24e-84 253
Msa0041280 MtrunA17_Chr1g0154351 53.036 247 108 4 12 251 8 253 1.62e-82 260
Msa0041280 MtrunA17_Chr1g0154311 52.869 244 107 4 13 250 10 251 6.73e-80 253
Msa0041280 MtrunA17_Chr1g0206531 51.639 244 110 5 9 250 13 250 2.04e-77 246
Msa0041280 MtrunA17_Chr5g0428591 48.770 244 117 4 10 251 8 245 1.34e-73 225
Msa0041280 MtrunA17_Chr1g0154371 50.391 256 115 5 1 246 8 261 1.91e-73 236
Msa0041280 MtrunA17_Chr1g0206461 47.934 242 119 3 9 250 13 247 4.84e-70 226
Msa0041280 MtrunA17_Chr1g0206451 49.794 243 115 4 9 249 47 284 2.58e-65 215
Msa0041280 MtrunA17_Chr5g0428571 48.246 228 112 3 24 251 25 246 5.35e-65 211
Msa0041280 MtrunA17_Chr1g0206521 48.560 243 117 4 9 250 655 890 1.92e-62 211
Msa0041280 MtrunA17_Chr1g0206521 47.679 237 116 4 15 250 19 248 3.60e-58 199
Msa0041280 MtrunA17_Chr5g0428531 43.725 247 126 5 10 250 8 247 3.77e-61 194
Msa0041280 MtrunA17_Chr1g0206561 41.600 250 135 5 4 252 1 240 1.95e-55 188
Msa0041280 MtrunA17_Chr5g0426941 41.564 243 128 5 14 252 10 242 3.47e-52 179
Msa0041280 MtrunA17_Chr1g0206381 38.589 241 133 6 21 252 19 253 1.23e-48 163
Msa0041280 MtrunA17_Chr1g0206401 39.189 222 121 6 39 252 33 248 2.35e-44 158
Msa0041280 MtrunA17_Chr7g0234231 31.818 242 156 4 9 250 5 237 5.44e-39 136
Msa0041280 MtrunA17_Chr1g0206331 34.959 246 148 7 11 251 15 253 1.22e-34 130
Msa0041280 MtrunA17_Chr5g0428601 50.400 125 60 2 127 251 1 123 4.66e-33 123
Msa0041280 MtrunA17_Chr6g0475121 33.462 260 157 6 1 252 1 252 5.46e-33 125
Msa0041280 MtrunA17_Chr6g0475121 32.353 204 126 6 50 250 296 490 5.73e-22 94.4
Msa0041280 MtrunA17_Chr2g0323011 31.325 249 156 7 4 249 7 243 6.74e-32 117
Msa0041280 MtrunA17_Chr1g0205771 31.925 213 139 3 41 251 51 259 7.09e-31 120
Msa0041280 MtrunA17_Chr5g0394001 32.231 242 155 6 13 250 13 249 3.92e-30 118
Msa0041280 MtrunA17_Chr6g0475201 31.395 258 164 5 1 252 1 251 4.92e-29 115
Msa0041280 MtrunA17_Chr6g0475201 32.489 237 139 8 20 251 271 491 2.10e-26 107
Msa0041280 MtrunA17_Chr5g0393981 29.845 258 160 8 1 250 73 317 6.31e-29 114
Msa0041280 MtrunA17_Chr1g0206201 33.486 218 127 6 43 252 48 255 6.75e-29 110
Msa0041280 MtrunA17_Chr6g0474901 29.961 257 163 7 1 252 1 245 8.50e-29 114
Msa0041280 MtrunA17_Chr6g0474901 30.992 242 141 8 19 250 258 483 3.89e-22 95.1
Msa0041280 MtrunA17_Chr6g0475001 31.274 259 164 7 1 252 1 252 1.90e-28 113
Msa0041280 MtrunA17_Chr6g0475001 31.556 225 136 8 28 250 281 489 2.88e-18 84.0
Msa0041280 MtrunA17_Chr2g0322981 29.880 251 161 7 4 251 7 245 5.98e-28 107
Msa0041280 MtrunA17_Chr5g0394021 34.298 242 149 5 11 250 14 247 2.91e-27 110
Msa0041280 MtrunA17_Chr6g0474811 30.651 261 164 6 1 252 1 253 3.21e-27 110
Msa0041280 MtrunA17_Chr6g0474811 32.843 204 125 6 50 250 298 492 7.12e-20 88.6
Msa0041280 MtrunA17_Chr6g0474971 30.451 266 150 8 1 252 1 245 3.94e-27 106
Msa0041280 MtrunA17_Chr1g0206351 32.075 212 138 3 43 251 46 254 4.12e-27 109
Msa0041280 MtrunA17_Chr8g0347571 31.907 257 153 9 1 250 1 242 3.20e-26 107
Msa0041280 MtrunA17_Chr8g0347571 28.194 227 142 8 32 251 276 488 6.06e-16 77.0
Msa0041280 MtrunA17_Chr3g0081051 31.092 238 153 5 14 249 11 239 4.35e-26 102
Msa0041280 MtrunA17_Chr3g0081001 29.388 245 158 5 11 251 13 246 4.70e-26 106
Msa0041280 MtrunA17_Chr3g0081031 32.677 254 155 8 1 250 17 258 1.13e-25 105
Msa0041280 MtrunA17_Chr6g0474871 30.620 258 165 6 1 252 1 250 1.19e-25 105
Msa0041280 MtrunA17_Chr6g0474871 30.882 204 129 6 50 250 293 487 1.58e-18 84.7
Msa0041280 MtrunA17_Chr1g0206231 32.381 210 132 6 47 252 52 255 1.29e-25 105
Msa0041280 MtrunA17_Chr3g0081011 30.088 226 144 5 30 251 30 245 6.25e-25 103
Msa0041280 MtrunA17_Chr6g0475111 29.412 255 169 6 1 250 4 252 7.47e-25 103
Msa0041280 MtrunA17_Chr6g0475111 26.316 247 163 8 14 251 266 502 5.52e-17 80.1
Msa0041280 MtrunA17_Chr1g0206361 31.651 218 138 6 37 250 43 253 2.75e-23 98.6
Msa0041280 MtrunA17_Chr8g0354271 29.918 244 158 7 12 251 15 249 2.97e-23 98.2
Msa0041280 MtrunA17_Chr6g0475091 30.962 239 155 5 20 252 27 261 4.26e-23 98.2
Msa0041280 MtrunA17_Chr6g0475091 33.498 203 125 6 50 250 305 499 6.24e-20 89.0
Msa0041280 MtrunA17_Chr6g0474861 32.549 255 155 10 1 250 1 243 5.76e-22 94.7
Msa0041280 MtrunA17_Chr5g0394011 30.556 216 139 6 41 250 41 251 8.45e-22 94.4
Msa0041280 MtrunA17_Chr1g0206291 29.389 262 164 9 2 252 15 266 2.08e-21 92.8
Msa0041280 MtrunA17_Chr8g0354351 27.459 244 161 7 11 251 13 243 2.16e-21 92.8
Msa0041280 MtrunA17_Chr1g0206251 31.754 211 129 7 47 250 307 509 1.12e-18 84.7
Msa0041280 MtrunA17_Chr1g0206251 29.902 204 123 10 61 251 69 265 6.63e-13 67.8
Msa0041280 MtrunA17_Chr2g0280391 26.087 230 157 5 24 250 27 246 4.98e-18 81.3
Msa0041280 MtrunA17_Chr8g0354421 25.984 254 169 8 4 251 30 270 7.85e-18 82.4
Msa0041280 MtrunA17_Chr8g0354341 31.325 166 107 4 86 251 88 246 2.04e-17 79.3
Msa0041280 MtrunA17_Chr1g0206411 56.897 58 25 0 61 118 4 61 3.78e-17 74.3
Msa0041280 MtrunA17_Chr1g0206421 30.189 159 93 5 67 216 2 151 6.55e-17 79.7
Msa0041280 MtrunA17_Chr3g0119041 29.808 208 118 8 64 251 63 262 3.00e-15 75.1
Msa0041280 MtrunA17_Chr8g0354411 25.000 216 146 6 42 250 41 247 3.56e-15 73.6
Msa0041280 MtrunA17_Chr2g0280361 24.898 245 164 7 10 250 13 241 4.29e-15 72.8
Msa0041280 MtrunA17_Chr8g0354441 23.529 255 168 10 10 250 14 255 5.02e-15 74.3
Msa0041280 MtrunA17_Chr7g0230381 25.258 194 126 4 58 251 74 248 8.86e-14 69.3
Msa0041280 MtrunA17_Chr8g0354281 24.057 212 148 6 45 251 44 247 1.06e-13 70.5
Msa0041280 MtrunA17_Chr3g0082951 39.091 110 62 3 28 136 116 221 1.67e-13 67.8
Msa0041280 MtrunA17_Chr8g0391511 22.749 211 144 5 38 244 39 234 3.24e-11 62.0
Msa0041280 MtrunA17_Chr8g0354431 24.900 249 171 8 10 251 9 248 4.36e-11 62.8
Msa0041280 MtrunA17_Chr2g0280381 23.280 189 133 4 65 252 65 242 7.66e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0041280 AT4G21410.2 38.073 218 128 4 37 251 38 251 7.98e-39 141
Msa0041280 AT4G21410.3 38.073 218 128 4 37 251 38 251 2.25e-38 142
Msa0041280 AT3G22060.1 34.884 215 134 3 38 252 43 251 4.30e-36 129
Msa0041280 AT4G38830.1 36.697 218 132 4 35 250 32 245 1.53e-35 134
Msa0041280 AT4G21400.3 33.953 215 137 3 39 251 42 253 2.80e-34 129
Msa0041280 AT4G21400.6 33.953 215 137 3 39 251 42 253 4.48e-34 129
Msa0041280 AT4G21400.4 33.953 215 137 3 39 251 42 253 4.48e-34 129
Msa0041280 AT4G21400.5 33.953 215 137 3 39 251 42 253 4.48e-34 129
Msa0041280 AT4G21400.2 33.953 215 137 3 39 251 42 253 6.83e-34 129
Msa0041280 AT4G21400.1 33.953 215 137 3 39 251 42 253 8.35e-34 129
Msa0041280 AT4G23220.1 32.243 214 144 1 39 251 29 242 2.54e-32 124
Msa0041280 AT4G23180.3 34.632 231 142 5 21 250 31 253 4.56e-32 123
Msa0041280 AT4G23180.2 34.632 231 142 5 21 250 31 253 6.89e-32 123
Msa0041280 AT4G23160.1 36.441 236 134 8 21 250 37 262 7.53e-32 123
Msa0041280 AT4G05200.2 34.263 251 150 8 5 250 26 266 8.23e-32 123
Msa0041280 AT4G05200.1 34.263 251 150 8 5 250 8 248 8.58e-32 123
Msa0041280 AT4G23160.3 36.441 236 134 8 21 250 617 842 1.07e-31 123
Msa0041280 AT4G23160.2 36.441 236 134 8 21 250 617 842 1.07e-31 123
Msa0041280 AT4G23180.1 34.632 231 142 5 21 250 31 253 1.21e-31 122
Msa0041280 AT4G21230.1 36.986 219 119 7 37 250 37 241 3.81e-31 121
Msa0041280 AT4G23170.1 35.443 237 135 9 21 250 20 245 4.60e-31 116
Msa0041280 AT4G23280.3 36.620 213 123 5 41 250 39 242 1.14e-30 120
Msa0041280 AT4G23280.1 36.620 213 123 5 41 250 39 242 1.27e-30 120
Msa0041280 AT4G23280.2 36.620 213 123 5 41 250 39 242 1.46e-30 119
Msa0041280 AT4G23200.2 31.308 214 142 2 41 249 30 243 2.38e-30 118
Msa0041280 AT4G23200.1 31.308 214 142 2 41 249 30 243 4.88e-30 118
Msa0041280 AT4G21400.7 35.429 175 109 2 78 251 56 227 8.27e-30 117
Msa0041280 AT4G00970.2 38.378 185 104 5 66 246 79 257 1.02e-29 117
Msa0041280 AT4G00970.1 38.378 185 104 5 66 246 79 257 1.60e-29 117
Msa0041280 AT4G23150.1 36.449 214 125 7 41 250 39 245 4.45e-29 115
Msa0041280 AT4G23130.3 34.762 210 126 6 45 251 44 245 1.58e-26 108
Msa0041280 AT4G23130.1 34.762 210 126 6 45 251 44 245 2.07e-26 107
Msa0041280 AT4G23130.2 34.762 210 126 6 45 251 44 245 2.16e-26 107
Msa0041280 AT4G23250.1 29.915 234 142 5 32 250 30 256 2.29e-26 107
Msa0041280 AT4G23250.3 29.915 234 142 5 32 250 30 256 2.66e-26 107
Msa0041280 AT4G23250.2 29.915 234 142 5 32 250 30 256 2.95e-26 107
Msa0041280 AT4G23250.2 26.471 204 125 4 47 250 741 919 1.83e-14 73.2
Msa0041280 AT4G23230.1 35.047 214 126 7 41 250 37 241 6.78e-26 106
Msa0041280 AT4G23140.2 34.259 216 127 7 41 250 40 246 1.04e-25 105
Msa0041280 AT4G23140.1 34.259 216 127 7 41 250 40 246 1.26e-25 105
Msa0041280 AT4G11530.1 27.888 251 159 5 4 245 5 242 5.28e-25 103
Msa0041280 AT3G45860.1 35.185 216 123 6 41 250 43 247 7.37e-25 103
Msa0041280 AT4G11460.3 29.545 220 145 4 39 249 32 250 1.62e-24 102
Msa0041280 AT4G11460.1 29.545 220 145 4 39 249 32 250 1.65e-24 102
Msa0041280 AT4G23210.1 28.761 226 143 4 37 249 30 250 3.09e-24 101
Msa0041280 AT4G23210.2 28.761 226 143 4 37 249 30 250 3.89e-24 100
Msa0041280 AT4G23210.4 28.761 226 143 4 37 249 30 250 3.89e-24 100
Msa0041280 AT4G23210.5 28.761 226 143 4 37 249 30 250 4.31e-24 100
Msa0041280 AT4G23210.3 28.761 226 143 4 37 249 30 250 4.38e-24 101
Msa0041280 AT4G23310.1 34.450 209 125 6 45 250 156 355 6.95e-24 100
Msa0041280 AT4G20640.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20630.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20580.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20600.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20620.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20610.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G20590.1 30.315 254 165 6 5 251 7 255 7.79e-24 97.1
Msa0041280 AT4G23310.2 34.450 209 125 6 45 250 156 355 7.91e-24 100
Msa0041280 AT4G20670.1 30.315 254 165 6 5 251 7 255 8.66e-24 97.1
Msa0041280 AT4G20570.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G20560.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G20540.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G20645.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G20530.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G20550.1 30.315 254 165 6 5 251 21 269 8.67e-24 97.1
Msa0041280 AT4G23300.1 28.761 226 143 4 37 249 30 250 9.30e-24 100
Msa0041280 AT3G58310.1 28.854 253 165 7 6 252 13 256 1.15e-23 96.3
Msa0041280 AT4G23240.1 30.918 207 138 3 47 250 41 245 1.67e-23 99.4
Msa0041280 AT4G23190.1 29.493 217 143 4 37 245 30 244 3.35e-23 98.6
Msa0041280 AT4G23190.2 29.493 217 143 4 37 245 30 244 3.65e-23 98.6
Msa0041280 AT4G23270.2 31.020 245 155 7 10 250 6 240 4.31e-23 98.2
Msa0041280 AT4G23270.1 31.020 245 155 7 10 250 6 240 4.31e-23 98.2
Msa0041280 AT4G23270.3 31.020 245 155 7 10 250 6 240 5.22e-23 98.2
Msa0041280 AT3G21990.1 30.204 245 158 6 13 251 18 255 6.46e-23 94.4
Msa0041280 AT5G48540.1 27.600 250 166 6 5 250 3 241 1.11e-22 94.0
Msa0041280 AT2G31620.1 29.358 218 145 4 36 249 40 252 1.14e-22 93.6
Msa0041280 AT4G11521.1 27.753 227 156 4 28 249 18 241 2.88e-22 92.8
Msa0041280 AT4G04500.1 29.384 211 134 5 55 252 47 255 2.15e-21 93.2
Msa0041280 AT4G04500.2 29.384 211 134 5 55 252 47 255 2.35e-21 93.2
Msa0041280 AT4G04500.3 29.384 211 134 5 55 252 47 255 2.35e-21 93.2
Msa0041280 AT4G11490.1 27.523 218 148 2 10 222 8 220 3.74e-21 92.8
Msa0041280 AT4G21410.1 41.732 127 71 2 127 251 1 126 2.04e-20 90.5
Msa0041280 AT1G61750.1 28.767 219 149 4 29 245 28 241 4.13e-20 88.2
Msa0041280 AT3G21960.1 29.730 222 144 5 36 251 46 261 4.84e-20 86.7
Msa0041280 AT4G21400.8 38.095 126 77 1 127 251 1 126 5.89e-20 89.0
Msa0041280 AT4G23260.2 26.009 223 158 3 32 249 30 250 1.93e-19 87.8
Msa0041280 AT4G23260.1 26.009 223 158 3 32 249 30 250 2.53e-19 87.4
Msa0041280 AT4G23290.2 28.959 221 143 7 38 249 31 246 6.96e-19 85.9
Msa0041280 AT3G21910.1 26.667 225 145 5 38 249 48 265 1.75e-18 82.8
Msa0041280 AT5G41290.1 26.230 244 166 9 9 246 8 243 2.25e-18 82.8
Msa0041280 AT4G11480.2 22.115 208 154 3 45 249 36 238 2.67e-18 84.0
Msa0041280 AT3G21940.1 30.935 278 148 12 5 252 12 275 3.20e-18 82.0
Msa0041280 AT4G11480.3 22.222 207 153 3 46 249 37 238 3.50e-18 84.0
Msa0041280 AT3G21940.2 34.973 183 96 6 88 252 3 180 3.60e-18 80.1
Msa0041280 AT4G11480.4 22.222 207 153 3 46 249 37 238 3.84e-18 84.0
Msa0041280 AT4G11480.1 22.222 207 153 3 46 249 37 238 4.00e-18 84.0
Msa0041280 AT4G11460.2 30.114 176 113 4 83 249 1 175 1.92e-17 81.6
Msa0041280 AT4G04570.1 26.761 213 148 3 45 250 40 251 2.07e-17 81.6
Msa0041280 AT4G04570.2 26.761 213 148 3 45 250 40 251 2.17e-17 81.6
Msa0041280 AT1G70690.1 26.104 249 157 10 3 245 6 233 5.08e-17 79.0
Msa0041280 AT3G21920.1 29.004 231 136 8 37 249 47 267 5.18e-17 79.0
Msa0041280 AT3G21920.2 29.004 231 136 8 37 249 69 289 6.45e-17 79.0
Msa0041280 AT1G63600.1 28.272 191 115 4 67 245 72 252 7.86e-17 79.0
Msa0041280 AT4G23190.3 29.167 168 110 3 85 245 1 166 1.25e-16 79.3
Msa0041280 AT4G23190.4 29.167 168 110 3 85 245 1 166 1.25e-16 79.3
Msa0041280 AT3G21930.1 28.571 231 137 8 37 249 47 267 2.41e-16 77.0
Msa0041280 AT3G29040.1 29.032 248 158 8 12 249 13 252 2.51e-16 76.6
Msa0041280 AT1G63550.1 24.255 235 145 5 26 246 30 245 2.64e-16 77.4
Msa0041280 AT4G04540.3 25.333 225 153 6 39 250 32 254 3.24e-16 78.2
Msa0041280 AT4G04540.4 25.333 225 153 6 39 250 32 254 3.48e-16 78.2
Msa0041280 AT4G04540.1 25.333 225 153 6 39 250 32 254 3.78e-16 78.2
Msa0041280 AT4G04540.2 25.333 225 153 6 39 250 32 254 4.40e-16 77.8
Msa0041280 AT3G22010.1 28.261 230 136 9 38 252 48 263 4.76e-16 75.9
Msa0041280 AT4G11470.1 26.761 213 143 6 47 250 38 246 9.27e-16 77.0
Msa0041280 AT4G11470.2 26.761 213 143 6 47 250 43 251 1.02e-15 76.6
Msa0041280 AT1G63570.3 26.984 189 117 3 68 246 64 241 1.39e-15 74.7
Msa0041280 AT1G63570.1 26.984 189 117 3 68 246 64 241 1.39e-15 74.7
Msa0041280 AT1G63570.2 26.984 189 117 3 68 246 117 294 2.06e-15 75.1
Msa0041280 AT5G41280.1 25.683 183 127 4 68 246 67 244 3.74e-15 73.6
Msa0041280 AT3G22040.1 26.577 222 148 6 38 252 44 257 5.04e-15 72.8
Msa0041280 AT4G04490.1 26.068 234 146 9 29 249 27 246 7.71e-15 74.3
Msa0041280 AT3G22050.1 29.293 198 110 7 67 249 73 255 1.60e-14 71.6
Msa0041280 AT4G20680.1 28.444 225 144 8 39 252 49 267 2.14e-14 71.2
Msa0041280 AT4G04510.1 25.792 221 147 4 41 250 34 248 2.34e-14 72.8
Msa0041280 AT4G04510.2 25.792 221 147 4 41 250 36 250 2.55e-14 72.8
Msa0041280 AT3G21970.1 28.077 260 164 10 4 249 11 261 3.58e-14 70.9
Msa0041280 AT5G41300.1 25.514 243 161 7 10 244 15 245 4.08e-14 70.9
Msa0041280 AT2G01660.2 26.357 258 152 11 1 247 2 232 7.02e-14 69.7
Msa0041280 AT3G29050.2 26.054 261 160 9 13 249 22 273 7.57e-14 70.1
Msa0041280 AT3G22000.1 28.696 230 137 10 39 252 49 267 9.96e-14 69.7
Msa0041280 AT2G01660.1 26.357 258 152 11 1 247 2 232 1.09e-13 69.7
Msa0041280 AT2G01660.3 26.357 258 152 11 1 247 2 232 1.14e-13 69.7
Msa0041280 AT1G63590.1 26.000 200 125 6 59 245 55 244 3.19e-13 68.2
Msa0041280 AT3G21900.2 24.900 249 175 6 1 249 11 247 6.81e-13 67.0
Msa0041280 AT1G63580.1 24.528 212 138 4 46 245 36 237 1.08e-12 66.6

Find 39 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTGTTGGTACAATATTT+TGG 0.104488 1_1:-71644997 None:intergenic
TATTCAGACCGTTCGATATT+AGG 0.205684 1_1:+71644715 Msa0041280:CDS
ATGAGAATGAGGAAGGAATA+TGG 0.272162 1_1:-71644349 None:intergenic
GGTTTACTTTGTCCTCGTTT+TGG 0.340580 1_1:-71644549 None:intergenic
AGACATTTGCGACAATCTTC+AGG 0.362672 1_1:-71644601 None:intergenic
GGTTTCTACAATACAAATTA+CGG 0.363088 1_1:+71644526 Msa0041280:CDS
AGTAGTGATCAAACAATATA+TGG 0.388142 1_1:+71644901 Msa0041280:CDS
TGGACATGCTTGTGTTAGAT+AGG 0.389375 1_1:-71644638 None:intergenic
AAATATTGTACCAACAAGAT+TGG 0.404642 1_1:+71645000 Msa0041280:CDS
AATTCTACAACCAATCTTGT+TGG 0.410248 1_1:-71645010 None:intergenic
TTAGAGCAAGCTGTTATTTG+AGG 0.415196 1_1:+71645034 Msa0041280:CDS
AAATGTGGCCTGCTATAATT+TGG 0.425646 1_1:+71644746 Msa0041280:CDS
ACAGTGTACACCTGATTTGT+CGG 0.437811 1_1:+71644930 Msa0041280:CDS
CCAAACAGAAAAGAAGCAAT+TGG 0.439075 1_1:+71644658 Msa0041280:CDS
CGTTTACTTTAGATGTGAAA+AGG 0.440701 1_1:-71644375 None:intergenic
CACTGTGAAACCGACAAATC+AGG 0.458317 1_1:-71644940 None:intergenic
TGAGAATGAGGAAGGAATAT+GGG 0.476426 1_1:-71644348 None:intergenic
GAATGTGAACAATGCAACTT+TGG 0.483957 1_1:+71644771 Msa0041280:CDS
TATATTGTTTGATCACTACT+AGG 0.502209 1_1:-71644898 None:intergenic
GAGGACAAAGTAAACCTCAT+TGG 0.502261 1_1:+71644556 Msa0041280:CDS
TATGCTGCAGGAACTTTGCC+AGG 0.503711 1_1:+71644874 Msa0041280:CDS
TTCAACTCAAAAGTTAACTA+CGG 0.507432 1_1:+71644505 Msa0041280:CDS
GATAGAGGCGACTACATAAC+CGG 0.514752 1_1:+71644436 Msa0041280:CDS
GTTGTTATGGTAGGTACTAC+CGG 0.518720 1_1:-71644455 None:intergenic
TGAAAAGGATTATGAGAATG+AGG 0.532845 1_1:-71644360 None:intergenic
ACATTCCACCAAATTATAGC+AGG 0.535057 1_1:-71644754 None:intergenic
AAGGATTATGAGAATGAGGA+AGG 0.539148 1_1:-71644356 None:intergenic
TCACATCGTAAGTATGCTGC+AGG 0.560986 1_1:+71644862 Msa0041280:CDS
TGTGGCCTGCTATAATTTGG+TGG 0.578852 1_1:+71644749 Msa0041280:CDS
GGCAGATCAATTCAATAGTG+TGG 0.581694 1_1:+71644792 Msa0041280:CDS
CTCCTCTACATATTCCAATG+AGG 0.587463 1_1:-71644570 None:intergenic
CGCTGAGGCCTAATATCGAA+CGG 0.598988 1_1:-71644723 None:intergenic
AACCTCATTGGAATATGTAG+AGG 0.614721 1_1:+71644568 Msa0041280:CDS
ACAGAAAAGAAGCAATTGGA+TGG 0.618085 1_1:+71644662 Msa0041280:CDS
GTTTGATCACTACTAGGACC+TGG 0.622316 1_1:-71644892 None:intergenic
TATTAGGCCTCAGCGAAATG+TGG 0.638853 1_1:+71644731 Msa0041280:CDS
TACAAATTACGGCCAAAACG+AGG 0.648241 1_1:+71644537 Msa0041280:CDS
AGAAGCAATTGGATGGTACG+AGG 0.694836 1_1:+71644669 Msa0041280:CDS
TAGCAGGCCACATTTCGCTG+AGG 0.701616 1_1:-71644738 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTGAATGATTTAAAAAACA+AGG + chr1_1:71644825-71644844 Msa0041280:CDS 20.0%
!! GTTGAATGATTTAAAAAACA+AGG + chr1_1:71644825-71644844 Msa0041280:CDS 20.0%
!!! GTTATTTTTGTCACATAAGT+AGG - chr1_1:71644416-71644435 None:intergenic 25.0%
! TTCAACTCAAAAGTTAACTA+CGG + chr1_1:71644505-71644524 Msa0041280:CDS 25.0%
! GGTTTCTACAATACAAATTA+CGG + chr1_1:71644526-71644545 Msa0041280:CDS 25.0%
! TTAAAAAACAAGGCTAGAAA+TGG + chr1_1:71644835-71644854 Msa0041280:CDS 25.0%
! TATATTGTTTGATCACTACT+AGG - chr1_1:71644901-71644920 None:intergenic 25.0%
! AGTAGTGATCAAACAATATA+TGG + chr1_1:71644901-71644920 Msa0041280:CDS 25.0%
!! TCTTGTTGGTACAATATTTT+GGG - chr1_1:71644999-71645018 None:intergenic 25.0%
!! ATCTTGTTGGTACAATATTT+TGG - chr1_1:71645000-71645019 None:intergenic 25.0%
! AAATATTGTACCAACAAGAT+TGG + chr1_1:71645000-71645019 Msa0041280:CDS 25.0%
!!! GTTATTTTTGTCACATAAGT+AGG - chr1_1:71644416-71644435 None:intergenic 25.0%
! TTCAACTCAAAAGTTAACTA+CGG + chr1_1:71644505-71644524 Msa0041280:CDS 25.0%
! GGTTTCTACAATACAAATTA+CGG + chr1_1:71644526-71644545 Msa0041280:CDS 25.0%
! TTAAAAAACAAGGCTAGAAA+TGG + chr1_1:71644835-71644854 Msa0041280:CDS 25.0%
! TATATTGTTTGATCACTACT+AGG - chr1_1:71644901-71644920 None:intergenic 25.0%
! AGTAGTGATCAAACAATATA+TGG + chr1_1:71644901-71644920 Msa0041280:CDS 25.0%
!! TCTTGTTGGTACAATATTTT+GGG - chr1_1:71644999-71645018 None:intergenic 25.0%
!! ATCTTGTTGGTACAATATTT+TGG - chr1_1:71645000-71645019 None:intergenic 25.0%
! AAATATTGTACCAACAAGAT+TGG + chr1_1:71645000-71645019 Msa0041280:CDS 25.0%
TGAAAAGGATTATGAGAATG+AGG - chr1_1:71644363-71644382 None:intergenic 30.0%
CGTTTACTTTAGATGTGAAA+AGG - chr1_1:71644378-71644397 None:intergenic 30.0%
TGTGACAAAAATAACGATAG+AGG + chr1_1:71644421-71644440 Msa0041280:CDS 30.0%
!!! TAACTTTTGAGTTGAAAGTG+AGG - chr1_1:71644501-71644520 None:intergenic 30.0%
AATTCTACAACCAATCTTGT+TGG - chr1_1:71645013-71645032 None:intergenic 30.0%
TGAAAAGGATTATGAGAATG+AGG - chr1_1:71644363-71644382 None:intergenic 30.0%
CGTTTACTTTAGATGTGAAA+AGG - chr1_1:71644378-71644397 None:intergenic 30.0%
TGTGACAAAAATAACGATAG+AGG + chr1_1:71644421-71644440 Msa0041280:CDS 30.0%
!!! TAACTTTTGAGTTGAAAGTG+AGG - chr1_1:71644501-71644520 None:intergenic 30.0%
AATTCTACAACCAATCTTGT+TGG - chr1_1:71645013-71645032 None:intergenic 30.0%
TGAGAATGAGGAAGGAATAT+GGG - chr1_1:71644351-71644370 None:intergenic 35.0%
ATGAGAATGAGGAAGGAATA+TGG - chr1_1:71644352-71644371 None:intergenic 35.0%
AAGGATTATGAGAATGAGGA+AGG - chr1_1:71644359-71644378 None:intergenic 35.0%
!!! GATTTTGAGGTTGTTATGGT+AGG - chr1_1:71644467-71644486 None:intergenic 35.0%
!!! AAGCGATTTTGAGGTTGTTA+TGG - chr1_1:71644471-71644490 None:intergenic 35.0%
! GGTATATGAAAGCGATTTTG+AGG - chr1_1:71644480-71644499 None:intergenic 35.0%
AACCTCATTGGAATATGTAG+AGG + chr1_1:71644568-71644587 Msa0041280:CDS 35.0%
! CCAATTGCTTCTTTTCTGTT+TGG - chr1_1:71644661-71644680 None:intergenic 35.0%
CCAAACAGAAAAGAAGCAAT+TGG + chr1_1:71644658-71644677 Msa0041280:CDS 35.0%
ACAGAAAAGAAGCAATTGGA+TGG + chr1_1:71644662-71644681 Msa0041280:CDS 35.0%
TATTCAGACCGTTCGATATT+AGG + chr1_1:71644715-71644734 Msa0041280:CDS 35.0%
AAATGTGGCCTGCTATAATT+TGG + chr1_1:71644746-71644765 Msa0041280:CDS 35.0%
ACATTCCACCAAATTATAGC+AGG - chr1_1:71644757-71644776 None:intergenic 35.0%
GAATGTGAACAATGCAACTT+TGG + chr1_1:71644771-71644790 Msa0041280:CDS 35.0%
! TTAGAGCAAGCTGTTATTTG+AGG + chr1_1:71645034-71645053 Msa0041280:CDS 35.0%
TGAGAATGAGGAAGGAATAT+GGG - chr1_1:71644351-71644370 None:intergenic 35.0%
ATGAGAATGAGGAAGGAATA+TGG - chr1_1:71644352-71644371 None:intergenic 35.0%
AAGGATTATGAGAATGAGGA+AGG - chr1_1:71644359-71644378 None:intergenic 35.0%
!!! GATTTTGAGGTTGTTATGGT+AGG - chr1_1:71644467-71644486 None:intergenic 35.0%
!!! AAGCGATTTTGAGGTTGTTA+TGG - chr1_1:71644471-71644490 None:intergenic 35.0%
! GGTATATGAAAGCGATTTTG+AGG - chr1_1:71644480-71644499 None:intergenic 35.0%
AACCTCATTGGAATATGTAG+AGG + chr1_1:71644568-71644587 Msa0041280:CDS 35.0%
! CCAATTGCTTCTTTTCTGTT+TGG - chr1_1:71644661-71644680 None:intergenic 35.0%
CCAAACAGAAAAGAAGCAAT+TGG + chr1_1:71644658-71644677 Msa0041280:CDS 35.0%
ACAGAAAAGAAGCAATTGGA+TGG + chr1_1:71644662-71644681 Msa0041280:CDS 35.0%
TATTCAGACCGTTCGATATT+AGG + chr1_1:71644715-71644734 Msa0041280:CDS 35.0%
AAATGTGGCCTGCTATAATT+TGG + chr1_1:71644746-71644765 Msa0041280:CDS 35.0%
ACATTCCACCAAATTATAGC+AGG - chr1_1:71644757-71644776 None:intergenic 35.0%
GAATGTGAACAATGCAACTT+TGG + chr1_1:71644771-71644790 Msa0041280:CDS 35.0%
! TTAGAGCAAGCTGTTATTTG+AGG + chr1_1:71645034-71645053 Msa0041280:CDS 35.0%
GTTGTTATGGTAGGTACTAC+CGG - chr1_1:71644458-71644477 None:intergenic 40.0%
TACAAATTACGGCCAAAACG+AGG + chr1_1:71644537-71644556 Msa0041280:CDS 40.0%
! GGTTTACTTTGTCCTCGTTT+TGG - chr1_1:71644552-71644571 None:intergenic 40.0%
GAGGACAAAGTAAACCTCAT+TGG + chr1_1:71644556-71644575 Msa0041280:CDS 40.0%
CTCCTCTACATATTCCAATG+AGG - chr1_1:71644573-71644592 None:intergenic 40.0%
AGACATTTGCGACAATCTTC+AGG - chr1_1:71644604-71644623 None:intergenic 40.0%
TGGACATGCTTGTGTTAGAT+AGG - chr1_1:71644641-71644660 None:intergenic 40.0%
GGCAGATCAATTCAATAGTG+TGG + chr1_1:71644792-71644811 Msa0041280:CDS 40.0%
! ACAGTGTACACCTGATTTGT+CGG + chr1_1:71644930-71644949 Msa0041280:CDS 40.0%
GTTGTTATGGTAGGTACTAC+CGG - chr1_1:71644458-71644477 None:intergenic 40.0%
TACAAATTACGGCCAAAACG+AGG + chr1_1:71644537-71644556 Msa0041280:CDS 40.0%
! GGTTTACTTTGTCCTCGTTT+TGG - chr1_1:71644552-71644571 None:intergenic 40.0%
GAGGACAAAGTAAACCTCAT+TGG + chr1_1:71644556-71644575 Msa0041280:CDS 40.0%
CTCCTCTACATATTCCAATG+AGG - chr1_1:71644573-71644592 None:intergenic 40.0%
AGACATTTGCGACAATCTTC+AGG - chr1_1:71644604-71644623 None:intergenic 40.0%
TGGACATGCTTGTGTTAGAT+AGG - chr1_1:71644641-71644660 None:intergenic 40.0%
GGCAGATCAATTCAATAGTG+TGG + chr1_1:71644792-71644811 Msa0041280:CDS 40.0%
! ACAGTGTACACCTGATTTGT+CGG + chr1_1:71644930-71644949 Msa0041280:CDS 40.0%
GATAGAGGCGACTACATAAC+CGG + chr1_1:71644436-71644455 Msa0041280:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG + chr1_1:71644669-71644688 Msa0041280:CDS 45.0%
TATTAGGCCTCAGCGAAATG+TGG + chr1_1:71644731-71644750 Msa0041280:CDS 45.0%
TGTGGCCTGCTATAATTTGG+TGG + chr1_1:71644749-71644768 Msa0041280:CDS 45.0%
TCACATCGTAAGTATGCTGC+AGG + chr1_1:71644862-71644881 Msa0041280:CDS 45.0%
GTTTGATCACTACTAGGACC+TGG - chr1_1:71644895-71644914 None:intergenic 45.0%
CACTGTGAAACCGACAAATC+AGG - chr1_1:71644943-71644962 None:intergenic 45.0%
GATAGAGGCGACTACATAAC+CGG + chr1_1:71644436-71644455 Msa0041280:CDS 45.0%
AGAAGCAATTGGATGGTACG+AGG + chr1_1:71644669-71644688 Msa0041280:CDS 45.0%
TATTAGGCCTCAGCGAAATG+TGG + chr1_1:71644731-71644750 Msa0041280:CDS 45.0%
TGTGGCCTGCTATAATTTGG+TGG + chr1_1:71644749-71644768 Msa0041280:CDS 45.0%
TCACATCGTAAGTATGCTGC+AGG + chr1_1:71644862-71644881 Msa0041280:CDS 45.0%
GTTTGATCACTACTAGGACC+TGG - chr1_1:71644895-71644914 None:intergenic 45.0%
CACTGTGAAACCGACAAATC+AGG - chr1_1:71644943-71644962 None:intergenic 45.0%
CGCTGAGGCCTAATATCGAA+CGG - chr1_1:71644726-71644745 None:intergenic 50.0%
TATGCTGCAGGAACTTTGCC+AGG + chr1_1:71644874-71644893 Msa0041280:CDS 50.0%
CGCTGAGGCCTAATATCGAA+CGG - chr1_1:71644726-71644745 None:intergenic 50.0%
TATGCTGCAGGAACTTTGCC+AGG + chr1_1:71644874-71644893 Msa0041280:CDS 50.0%
TAGCAGGCCACATTTCGCTG+AGG - chr1_1:71644741-71644760 None:intergenic 55.0%
TAGCAGGCCACATTTCGCTG+AGG - chr1_1:71644741-71644760 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 71644328 71645086 71644328 ID=Msa0041280;Name=Msa0041280
chr1_1 mRNA 71644328 71645086 71644328 ID=Msa0041280-mRNA-1;Parent=Msa0041280;Name=Msa0041280-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|252
chr1_1 exon 71644328 71645086 71644328 ID=Msa0041280-mRNA-1:exon:23733;Parent=Msa0041280-mRNA-1
chr1_1 CDS 71644328 71645086 71644328 ID=Msa0041280-mRNA-1:cds;Parent=Msa0041280-mRNA-1
Gene Sequence

>Msa0041280

ATGGCTACTATTTCTGTAATCCCATATTCCTTCCTCATTCTCATAATCCTTTTCACATCTAAAGTAAACGCAGATGACGAGTTCACCTACTTATGTGACAAAAATAACGATAGAGGCGACTACATAACCGGTAGTACCTACCATAACAACCTCAAAATCGCTTTCATATACCTCACTTTCAACTCAAAAGTTAACTACGGTTTCTACAATACAAATTACGGCCAAAACGAGGACAAAGTAAACCTCATTGGAATATGTAGAGGAGACATTAATCCTGAAGATTGTCGCAAATGTCTCGTTAGTTCAAAATCCTATCTAACACAAGCATGTCCAAACAGAAAAGAAGCAATTGGATGGTACGAGGCTGATCAGAAATGCATGTTGCGTTATTCAGACCGTTCGATATTAGGCCTCAGCGAAATGTGGCCTGCTATAATTTGGTGGAATGTGAACAATGCAACTTTGGCAGATCAATTCAATAGTGTGGCGAAACAATTGTTGAATGATTTAAAAAACAAGGCTAGAAATGGTGACTCACATCGTAAGTATGCTGCAGGAACTTTGCCAGGTCCTAGTAGTGATCAAACAATATATGGTCTTGTACAGTGTACACCTGATTTGTCGGTTTCACAGTGTGATGACTGTTTGAATGAATCTATTGCTGAAGTCCCAAAATATTGTACCAACAAGATTGGTTGTAGAATTTTTAGAGCAAGCTGTTATTTGAGGTTTGAAACTCAACAATTCTATCAAACCTAA
Protein sequence

>Msa0041280

MATISVIPYSFLILIILFTSKVNADDEFTYLCDKNNDRGDYITGSTYHNNLKIAFIYLTFNSKVNYGFYNTNYGQNEDKVNLIGICRGDINPEDCRKCLVSSKSYLTQACPNRKEAIGWYEADQKCMLRYSDRSILGLSEMWPAIIWWNVNNATLADQFNSVAKQLLNDLKNKARNGDSHRKYAAGTLPGPSSDQTIYGLVQCTPDLSVSQCDDCLNESIAEVPKYCTNKIGCRIFRASCYLRFETQQFYQT*