AlfalfaGEDB | Msa0059910

M. sativa cultivar zhongmu-4 / Msa0059910

KEGG: K13494|K13495
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0006464 GO:0006486 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009308 GO:0009653 GO:0009690 GO:0009791 GO:0009888 GO:0009889 GO:0009908 GO:0009965 GO:0009987 GO:0010015 GO:0010016 GO:0010073 GO:0010075 GO:0010083 GO:0010154 GO:0010380 GO:0010817 GO:0016740 GO:0016757 GO:0016758 GO:0016763 GO:0019222 GO:0019538 GO:0022414 GO:0022622 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034645 GO:0034754 GO:0035251 GO:0035252 GO:0036211 GO:0040008 GO:0042285 GO:0042445 GO:0043170 GO:0043412 GO:0043413 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0045926 GO:0046483 GO:0046527 GO:0048316 GO:0048364 GO:0048366 GO:0048367 GO:0048439 GO:0048507 GO:0048509 GO:0048519 GO:0048608 GO:0048638 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050403 GO:0050404 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051193 GO:0051239 GO:0061458 GO:0065007 GO:0065008 GO:0070085 GO:0071704 GO:0090056 GO:0090567 GO:0090698 GO:0099402 GO:1901135 GO:1901137 GO:1901401 GO:1901463 GO:1901564 GO:1901566 GO:1901576 GO:1905392 GO:1905613 GO:2000026
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059910	XP_013466442.1	87.425	167	15	1	1	167	1	161	1.23e-92	286

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059910	sp\|Q9ZSK5\|ZOG_PHALU	62.025	158	52	3	10	167	11	160	2.86e-53	178

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059910	A0A072VGD7	87.425	167	15	1	1	167	1	161	5.87e-93	286

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0059900	Msa0059910	0.840132	1.045128e-57	-8.615850e-47
Msa0059910	Msa0104810	0.800232	1.587195e-48	-8.615850e-47
Msa0059910	Msa0119440	0.803261	3.793488e-49	-8.615850e-47
Msa0059910	Msa0187310	0.818850	1.580777e-52	-8.615850e-47
Msa0059910	Msa0364790	0.808604	2.855060e-50	-8.615850e-47
Msa0059910	Msa0524330	0.807610	4.646331e-50	-8.615850e-47
Msa0059910	Msa0968050	0.802745	4.848421e-49	-8.615850e-47
Msa0059910	Msa1214170	0.803232	3.846724e-49	-8.615850e-47
Msa0059910	Msa1285200	0.801773	7.685881e-49	-8.615850e-47
Msa0059910	Msa1360020	0.804226	2.391884e-49	-8.615850e-47
Msa0059910	Msa1367530	0.804405	2.195014e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0059910	MtrunA17_Chr1g0159551	87.425	167	15	1	1	167	1	161	1.13e-96	286
Msa0059910	MtrunA17_Chr1g0173121	85.030	167	23	1	1	167	1	165	1.97e-87	263
Msa0059910	MtrunA17_Chr8g0382021	66.879	157	51	1	10	166	30	185	3.92e-64	204

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 41 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAAATTTAGTTTCTGCATT+TGG	0.256117	1_2:+17420662	None:intergenic
TTTGAGATTGAAATGGAGTT+TGG	0.282536	1_2:+17420737	None:intergenic
GAACTTCGAAGTGATGAAAA+TGG	0.335131	1_2:+17420711	None:intergenic
AACTAAATTTCCTTCTCATA+TGG	0.340746	1_2:-17420651	Msa0059910:CDS
TGTAATTCATGACACATATA+TGG	0.368834	1_2:-17420543	Msa0059910:CDS
GAAATTTAGTTTCTGCATTT+GGG	0.372024	1_2:+17420663	None:intergenic
TTAGTTTCTGCATTTGGGTT+AGG	0.391981	1_2:+17420668	None:intergenic
TTTGGAATTGCTTTGGCCAT+TGG	0.414251	1_2:+17420919	None:intergenic
ATGGATGTTTGAGATTGAAA+TGG	0.424877	1_2:+17420730	None:intergenic
TGATTCAAATGGCCTTGTGC+TGG	0.429358	1_2:+17420866	None:intergenic
AAACCAAGTGGTGATTGTTA+TGG	0.444236	1_2:-17420897	Msa0059910:CDS
AACATACCTGTCCATTATGT+TGG	0.450291	1_2:-17420809	Msa0059910:CDS
TACAATTCGTGGCCAAGGTT+CGG	0.451038	1_2:-17420762	Msa0059910:CDS
GTATAGAAGTCCCAACATAA+TGG	0.469536	1_2:+17420798	None:intergenic
ACATACCTGTCCATTATGTT+GGG	0.490823	1_2:-17420808	Msa0059910:CDS
GTTTCTGCATTTGGGTTAGG+AGG	0.502226	1_2:+17420671	None:intergenic
TCTTCCCATTCACTCCCAAA+TGG	0.502362	1_2:+17420436	None:intergenic
GGTGTAGAAGCTGATTCAAA+TGG	0.504630	1_2:+17420855	None:intergenic
TCTGCATTTGGGTTAGGAGG+AGG	0.518205	1_2:+17420674	None:intergenic
TGGAGTTTGGATCCGAACCT+TGG	0.531258	1_2:+17420750	None:intergenic
AATCGTCAAGCTACAATTCG+TGG	0.533085	1_2:-17420773	Msa0059910:CDS
GATTCAAATGGCCTTGTGCT+GGG	0.534487	1_2:+17420867	None:intergenic
CCCATTCACTCCCAAATGGA+AGG	0.535019	1_2:+17420440	None:intergenic
TTAGGAGGAGGTGAAACAAA+GGG	0.545800	1_2:+17420686	None:intergenic
TGTAAAGAGGCTTCAAAAGA+CGG	0.554973	1_2:+17420626	None:intergenic
GAAGTCCCAACATAATGGAC+AGG	0.557790	1_2:+17420803	None:intergenic
GTTAGGAGGAGGTGAAACAA+AGG	0.561610	1_2:+17420685	None:intergenic
CAAGCTACAATTCGTGGCCA+AGG	0.562846	1_2:-17420767	Msa0059910:CDS
AAATGGCCTTGTGCTGGGAA+AGG	0.563121	1_2:+17420872	None:intergenic
GATTGCATGAGTTCCGCCAC+AGG	0.565307	1_2:+17420593	None:intergenic
ACATCTTCGCGAGCCTGTGG+CGG	0.571072	1_2:-17420606	Msa0059910:CDS
ATGGTACCTTTCCCAGCACA+AGG	0.595372	1_2:-17420878	Msa0059910:CDS
TTTACATCTTCGCGAGCCTG+TGG	0.604307	1_2:-17420609	Msa0059910:CDS
CCATTCACTCCCAAATGGAA+GGG	0.607272	1_2:+17420441	None:intergenic
AAAGACGGAACCATATGAGA+AGG	0.610573	1_2:+17420641	None:intergenic
AATTCACTAATGGCTTCCAA+TGG	0.622620	1_2:-17420935	None:intergenic
GTACCATAACAATCACCACT+TGG	0.627734	1_2:+17420894	None:intergenic
AGGCTCGCGAAGATGTAAAG+AGG	0.628262	1_2:+17420613	None:intergenic
TGACACATATATGGCATCAG+TGG	0.637244	1_2:-17420534	Msa0059910:CDS
GTGATAAGATTAAGCGTGAG+AGG	0.673993	1_2:+17420834	None:intergenic
GGAGGAGGTGAAACAAAGGG+AGG	0.734329	1_2:+17420689	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAATTTAGTTTCTGCATTT+GGG	+	chr1_2:17420711-17420730	None:intergenic	25.0%
!	AACTAAATTTCCTTCTCATA+TGG	-	chr1_2:17420720-17420739	Msa0059910:CDS	25.0%
!!	ACTTTCATCTGTTTTCAAAA+GGG	-	chr1_2:17420801-17420820	Msa0059910:CDS	25.0%
!	TGTAATTCATGACACATATA+TGG	-	chr1_2:17420828-17420847	Msa0059910:CDS	25.0%
!	TTTGAGATTGAAATGGAGTT+TGG	+	chr1_2:17420637-17420656	None:intergenic	30.0%
!	ATGGATGTTTGAGATTGAAA+TGG	+	chr1_2:17420644-17420663	None:intergenic	30.0%
!	GGAAATTTAGTTTCTGCATT+TGG	+	chr1_2:17420712-17420731	None:intergenic	30.0%
!	CACTTTCATCTGTTTTCAAA+AGG	-	chr1_2:17420800-17420819	Msa0059910:CDS	30.0%
!!!	CTTGGTTTTTGGAATTGCTT+TGG	+	chr1_2:17420462-17420481	None:intergenic	35.0%
	AAGCAATTCCAAAAACCAAG+TGG	-	chr1_2:17420462-17420481	Msa0059910:CDS	35.0%
!!	AACAATCACCACTTGGTTTT+TGG	+	chr1_2:17420473-17420492	None:intergenic	35.0%
	AAACCAAGTGGTGATTGTTA+TGG	-	chr1_2:17420474-17420493	Msa0059910:CDS	35.0%
	AACATACCTGTCCATTATGT+TGG	-	chr1_2:17420562-17420581	Msa0059910:CDS	35.0%
	ACATACCTGTCCATTATGTT+GGG	-	chr1_2:17420563-17420582	Msa0059910:CDS	35.0%
	GTATAGAAGTCCCAACATAA+TGG	+	chr1_2:17420576-17420595	None:intergenic	35.0%
	GAACTTCGAAGTGATGAAAA+TGG	+	chr1_2:17420663-17420682	None:intergenic	35.0%
!	TTAGTTTCTGCATTTGGGTT+AGG	+	chr1_2:17420706-17420725	None:intergenic	35.0%
	TGTAAAGAGGCTTCAAAAGA+CGG	+	chr1_2:17420748-17420767	None:intergenic	35.0%
!	TTACAGTTTTCCCTTCCATT+TGG	-	chr1_2:17420920-17420939	Msa0059910:CDS	35.0%
!	TACAGTTTTCCCTTCCATTT+GGG	-	chr1_2:17420921-17420940	Msa0059910:CDS	35.0%
!	TTTGGAATTGCTTTGGCCAT+TGG	+	chr1_2:17420455-17420474	None:intergenic	40.0%
	GTACCATAACAATCACCACT+TGG	+	chr1_2:17420480-17420499	None:intergenic	40.0%
!	GGTGTAGAAGCTGATTCAAA+TGG	+	chr1_2:17420519-17420538	None:intergenic	40.0%
	GTGATAAGATTAAGCGTGAG+AGG	+	chr1_2:17420540-17420559	None:intergenic	40.0%
	AATCGTCAAGCTACAATTCG+TGG	-	chr1_2:17420598-17420617	Msa0059910:CDS	40.0%
	TTAGGAGGAGGTGAAACAAA+GGG	+	chr1_2:17420688-17420707	None:intergenic	40.0%
	AAAGACGGAACCATATGAGA+AGG	+	chr1_2:17420733-17420752	None:intergenic	40.0%
	TGACACATATATGGCATCAG+TGG	-	chr1_2:17420837-17420856	Msa0059910:CDS	40.0%
!!	GATTCAAATGGCCTTGTGCT+GGG	+	chr1_2:17420507-17420526	None:intergenic	45.0%
!!	TGATTCAAATGGCCTTGTGC+TGG	+	chr1_2:17420508-17420527	None:intergenic	45.0%
!!	GAAGTCCCAACATAATGGAC+AGG	+	chr1_2:17420571-17420590	None:intergenic	45.0%
	TACAATTCGTGGCCAAGGTT+CGG	-	chr1_2:17420609-17420628	Msa0059910:CDS	45.0%
	GTTAGGAGGAGGTGAAACAA+AGG	+	chr1_2:17420689-17420708	None:intergenic	45.0%
!	GTTTCTGCATTTGGGTTAGG+AGG	+	chr1_2:17420703-17420722	None:intergenic	45.0%
	ATGGTACCTTTCCCAGCACA+AGG	-	chr1_2:17420493-17420512	Msa0059910:CDS	50.0%
!!	AAATGGCCTTGTGCTGGGAA+AGG	+	chr1_2:17420502-17420521	None:intergenic	50.0%
	CAAGCTACAATTCGTGGCCA+AGG	-	chr1_2:17420604-17420623	Msa0059910:CDS	50.0%
	TGGAGTTTGGATCCGAACCT+TGG	+	chr1_2:17420624-17420643	None:intergenic	50.0%
!	TCTGCATTTGGGTTAGGAGG+AGG	+	chr1_2:17420700-17420719	None:intergenic	50.0%
	AGGCTCGCGAAGATGTAAAG+AGG	+	chr1_2:17420761-17420780	None:intergenic	50.0%
	TTTACATCTTCGCGAGCCTG+TGG	-	chr1_2:17420762-17420781	Msa0059910:CDS	50.0%
	GGAGGAGGTGAAACAAAGGG+AGG	+	chr1_2:17420685-17420704	None:intergenic	55.0%
	GATTGCATGAGTTCCGCCAC+AGG	+	chr1_2:17420781-17420800	None:intergenic	55.0%
	ACATCTTCGCGAGCCTGTGG+CGG	-	chr1_2:17420765-17420784	Msa0059910:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	17420445	17420948	17420445	ID=Msa0059910;Name=Msa0059910
chr1_2	mRNA	17420445	17420948	17420445	ID=Msa0059910-mRNA-1;Parent=Msa0059910;Name=Msa0059910-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|167
chr1_2	exon	17420445	17420948	17420445	ID=Msa0059910-mRNA-1:exon:6765;Parent=Msa0059910-mRNA-1
chr1_2	CDS	17420445	17420948	17420445	ID=Msa0059910-mRNA-1:cds;Parent=Msa0059910-mRNA-1

Gene Sequence

>Msa0059910

ATGGCTTCCAATGGCCAAAGCAATTCCAAAAACCAAGTGGTGATTGTTATGGTACCTTTCCCAGCACAAGGCCATTTGAATCAGCTTCTACACCTCTCACGCTTAATCTTATCACACAACATACCTGTCCATTATGTTGGGACTTCTATACACAATCGTCAAGCTACAATTCGTGGCCAAGGTTCGGATCCAAACTCCATTTCAATCTCAAACATCCATTTTCATCACTTCGAAGTTCCTCCCTTTGTTTCACCTCCTCCTAACCCAAATGCAGAAACTAAATTTCCTTCTCATATGGTTCCGTCTTTTGAAGCCTCTTTACATCTTCGCGAGCCTGTGGCGGAACTCATGCAATCACTTTCATCTGTTTTCAAAAGGGTCGTTGTAATTCATGACACATATATGGCATCAGTGGTACAAGATGCTATAAACATAGCAAATGTTGAGAATTACACATTTTTTAGCACATGTGCTTTTACAGTTTTCCCTTCCATTTGGGAGTGA

Protein sequence

>Msa0059910

MASNGQSNSKNQVVIVMVPFPAQGHLNQLLHLSRLILSHNIPVHYVGTSIHNRQATIRGQGSDPNSISISNIHFHHFEVPPFVSPPPNPNAETKFPSHMVPSFEASLHLREPVAELMQSLSSVFKRVVVIHDTYMASVVQDAINIANVENYTFFSTCAFTVFPSIWE*