AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0067280

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067280 MtrunA17_Chr1g0171071 93.678 348 22 0 1 348 1 348 0.0 682
Msa0067280 MtrunA17_Chr1g0210571 59.366 347 125 2 1 347 1 331 1.01e-146 417
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0067280 AT3G54290.1 40.701 371 165 4 1 343 1 344 5.29e-93 281

Find 138 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTCCACTGCCTCCATTAA+TGG 0.185489 1_2:+32225729 None:intergenic
GTACAAAGTCTCTCTGTAAT+TGG 0.218096 1_2:+32226153 None:intergenic
GTTTATTGACGCGCGGTTTC+CGG 0.224860 1_2:-32227516 Msa0067280:CDS
TATCAAGCTCAATTCATTTC+AGG 0.244843 1_2:-32226122 Msa0067280:intron
TTTGGAATTACTTCCATTAA+TGG 0.256564 1_2:-32225742 Msa0067280:CDS
TGTCCGAGGAAGCAGTATTT+TGG 0.262383 1_2:+32227672 None:intergenic
AATGCTGTTTCAAAATAAAA+AGG 0.289237 1_2:+32225395 None:intergenic
ACACTTCATGGAAGAGGATT+TGG 0.299013 1_2:-32225760 Msa0067280:CDS
GTTGGGTGTGGAAGCAATTT+CGG 0.303130 1_2:+32227918 None:intergenic
TTGGGTGTGGAAGCAATTTC+GGG 0.303934 1_2:+32227919 None:intergenic
CGCGTGGGTTGCAACCCAAC+TGG 0.305989 1_2:-32227604 Msa0067280:CDS
ATGTACTTCATTGCATCATT+AGG 0.328321 1_2:+32225609 None:intergenic
GTGCAGGGACAGAGAGAAAA+TGG 0.331008 1_2:-32225571 Msa0067280:CDS
CAACTGGGAGAATTCGATTA+AGG 0.331298 1_2:-32227588 Msa0067280:CDS
TACAAAGTCTCTCTGTAATT+GGG 0.338823 1_2:+32226154 None:intergenic
CAACTTAGAGGGGCTCAAAT+AGG 0.352166 1_2:-32225261 Msa0067280:three_prime_UTR
TTGTTAAAGTTCCTTGTTGA+AGG 0.357583 1_2:-32225639 Msa0067280:CDS
TAAGAGCGTGACGTGGCATT+TGG 0.364488 1_2:-32227387 Msa0067280:CDS
CTAATCCTTCTCAAAGTAAT+GGG 0.376989 1_2:-32227827 Msa0067280:exon
AAAGTGAGTGTACTAACATA+TGG 0.387215 1_2:+32228016 None:intergenic
GAAGAAAGAAGAAGGATAGA+AGG 0.388111 1_2:+32227941 None:intergenic
GGTGAGATGGGCGGAAGATC+TGG 0.390752 1_2:-32227357 Msa0067280:CDS
ATGCATTACAAAAGAAATAC+AGG 0.406304 1_2:-32225368 Msa0067280:three_prime_UTR
TGTGAGTGCAGGGCTATCTA+AGG 0.406854 1_2:-32226266 Msa0067280:intron
TAAACGGTGGCTCCGTCGTT+GGG 0.412707 1_2:+32227761 None:intergenic
GACTCGAAGTCCGACTTCAT+TGG 0.413120 1_2:+32227563 None:intergenic
ATCTTCCAACTCCCCATCTT+CGG 0.414500 1_2:+32227305 None:intergenic
TGTCCGGTGTGCCGTGTAAA+CGG 0.414939 1_2:+32227729 None:intergenic
TAAACGGACGGTGGGGTAAA+CGG 0.426273 1_2:+32227745 None:intergenic
GTAAACGGTGGCTCCGTCGT+TGG 0.427517 1_2:+32227760 None:intergenic
TCTTTGATGCCATTCATGAT+AGG 0.436853 1_2:+32226214 None:intergenic
CCGTATAAAACGGCGGCGCG+TGG 0.437111 1_2:-32227620 Msa0067280:CDS
ACAATGCAAACAACACTTCA+TGG 0.440413 1_2:-32225772 Msa0067280:CDS
ACCGTCGTCGTCGGTGGTGC+CGG 0.445068 1_2:-32227477 Msa0067280:CDS
ATGAAGTCGGACTTCGAGTC+AGG 0.446510 1_2:-32227560 Msa0067280:CDS
TCTAATCCTTCTCAAAGTAA+TGG 0.447554 1_2:-32227828 Msa0067280:exon
TTTGGAGAGGTTGGTGAGAT+GGG 0.451450 1_2:-32227369 Msa0067280:CDS
TACTCTTCATCACATGATCA+TGG 0.456975 1_2:-32225515 Msa0067280:three_prime_UTR
TTTGAGCCCCTCTAAGTTGG+TGG 0.458712 1_2:+32225265 None:intergenic
CAGGGAAATGGTGTTAGAAT+CGG 0.462615 1_2:+32227889 None:intergenic
ATTTGGAGAGGTTGGTGAGA+TGG 0.466097 1_2:-32227370 Msa0067280:CDS
GTAGGGACCCACCAACTTAG+AGG 0.473961 1_2:-32225273 Msa0067280:three_prime_UTR
GGATGCAACGACGTCGTTGA+TGG 0.474526 1_2:-32227432 Msa0067280:CDS
ATCCATGTGAGTGTTATAGT+TGG 0.475549 1_2:+32225490 None:intergenic
ATACGGCGGAGGTGACTGTC+TGG 0.475784 1_2:+32227637 None:intergenic
CGCGCGGTTTCCGGAGCTGT+CGG 0.476641 1_2:-32227507 Msa0067280:CDS
AATCGGAATGTTGTTGGGTG+TGG 0.477346 1_2:+32227906 None:intergenic
GATGATGGAGCATGCTCAAA+TGG 0.480391 1_2:-32227237 Msa0067280:CDS
AAGAAGCTACACTCAATTGC+TGG 0.483739 1_2:-32227264 Msa0067280:CDS
GTCACCTCCGCCGTATAAAA+CGG 0.483937 1_2:-32227630 Msa0067280:CDS
CTATTTGAGCCCCTCTAAGT+TGG 0.484762 1_2:+32225262 None:intergenic
GAAGATGGGGAGTTGGAAGA+TGG 0.493642 1_2:-32227303 Msa0067280:CDS
CTTAATCGAATTCTCCCAGT+TGG 0.494401 1_2:+32227589 None:intergenic
TCAATTGCTGGAAGTGATGA+TGG 0.494435 1_2:-32227252 Msa0067280:CDS
GGTTCCTCAGCCGACAGCTC+CGG 0.494916 1_2:+32227497 None:intergenic
CGTATAAAACGGCGGCGCGT+GGG 0.496687 1_2:-32227619 Msa0067280:CDS
AGAAATACAGGGGTATTTCA+AGG 0.496825 1_2:-32225356 Msa0067280:three_prime_UTR
GCTCCCGCGACGCGCTTCTA+CGG 0.496834 1_2:-32227538 Msa0067280:CDS
TGTTAGAATCGGAATGTTGT+TGG 0.499908 1_2:+32227900 None:intergenic
TTAATCGAATTCTCCCAGTT+GGG 0.501407 1_2:+32227590 None:intergenic
GTTTCCGGAGCTGTCGGCTG+AGG 0.501540 1_2:-32227501 Msa0067280:CDS
GTTAGAATCGGAATGTTGTT+GGG 0.504900 1_2:+32227901 None:intergenic
TACCCCACCGTCCGTTTACA+CGG 0.505411 1_2:-32227740 Msa0067280:CDS
GAAAGAAGAAGGATAGAAGG+TGG 0.509074 1_2:+32227944 None:intergenic
GGCTGAGGAACCGTCGTCGT+CGG 0.510268 1_2:-32227486 Msa0067280:CDS
GGATTAGATTGCAATTAATG+TGG 0.515150 1_2:+32227843 None:intergenic
GAAGGCCGTTGATCCGAAGA+TGG 0.519421 1_2:-32227318 Msa0067280:CDS
TGTTAAAGTTCCTTGTTGAA+GGG 0.519804 1_2:-32225638 Msa0067280:CDS
AAACCGTAGAAGCGCGTCGC+GGG 0.525531 1_2:+32227535 None:intergenic
TTGAGCCCCTCTAAGTTGGT+GGG 0.527283 1_2:+32225266 None:intergenic
ACTTCCATTAATGGAGGCAG+TGG 0.529863 1_2:-32225733 Msa0067280:CDS
AAGTAGAATGTCAAAACAGT+AGG 0.531776 1_2:-32225291 Msa0067280:three_prime_UTR
TCTCCAAAATACTGCTTCCT+CGG 0.536265 1_2:-32227675 Msa0067280:CDS
AAGGCCGTTGATCCGAAGAT+GGG 0.537645 1_2:-32227317 Msa0067280:CDS
CAAACAACACTTCATGGAAG+AGG 0.537849 1_2:-32225766 Msa0067280:CDS
GTGCCGTGTAAACGGACGGT+GGG 0.539018 1_2:+32227737 None:intergenic
TGGACTTGATCAGCATGTGC+AGG 0.540032 1_2:-32225587 Msa0067280:CDS
AGTTTCCCATTACTTTGAGA+AGG 0.545746 1_2:+32227822 None:intergenic
TCGGTGGTGCCGGTGAGTGA+TGG 0.547568 1_2:-32227467 Msa0067280:CDS
GGCGACACGTGGAGGGAGGA+AGG 0.548364 1_2:-32227336 Msa0067280:CDS
AGAAATCATCAAATCTGTTG+AGG 0.551038 1_2:-32226194 Msa0067280:CDS
GCTATCTAAGGCTGCAAAGG+AGG 0.554055 1_2:-32226254 Msa0067280:CDS
CTGCAAAGGAGGAACATGCT+AGG 0.554166 1_2:-32226243 Msa0067280:CDS
TAGGGACCCACCAACTTAGA+GGG 0.555370 1_2:-32225272 Msa0067280:three_prime_UTR
TGCAAAGGAGGAACATGCTA+GGG 0.560546 1_2:-32226242 Msa0067280:CDS
TTGATCCGAAGATGGGGAGT+TGG 0.561093 1_2:-32227310 Msa0067280:CDS
AAGTGAGTGTACTAACATAT+GGG 0.566040 1_2:+32228017 None:intergenic
TGATGCCATTCATGATAGGT+AGG 0.566617 1_2:+32226218 None:intergenic
TGAGGAACCGTCGTCGTCGG+TGG 0.567151 1_2:-32227483 Msa0067280:CDS
AGGGACCTACCTATCATGAA+TGG 0.568429 1_2:-32226223 Msa0067280:CDS
GACGTGGCATTTGGAGAGGT+TGG 0.574473 1_2:-32227378 Msa0067280:CDS
GGAGAGGTTGGTGAGATGGG+CGG 0.575021 1_2:-32227366 Msa0067280:CDS
TAATGATGCAATGAAGTACA+TGG 0.577202 1_2:-32225607 Msa0067280:CDS
GATGGGGAGTTGGAAGATGG+AGG 0.581139 1_2:-32227300 Msa0067280:CDS
GCGTGGGTTGCAACCCAACT+GGG 0.581931 1_2:-32227603 Msa0067280:CDS
ATCTGGCGACACGTGGAGGG+AGG 0.588045 1_2:-32227340 Msa0067280:CDS
ACTCTTCATCACATGATCAT+GGG 0.589357 1_2:-32225514 Msa0067280:three_prime_UTR
TATGTAGAGAAAATACTGTC+CGG 0.590833 1_2:+32227713 None:intergenic
GATTAAGGTTCCAATGAAGT+CGG 0.592671 1_2:-32227573 Msa0067280:CDS
AGGGCTATCTAAGGCTGCAA+AGG 0.592821 1_2:-32226257 Msa0067280:CDS
ACAAAGTCTCTCTGTAATTG+GGG 0.596977 1_2:+32226155 None:intergenic
AAGATCTGGCGACACGTGGA+GGG 0.597809 1_2:-32227343 Msa0067280:CDS
AGTAGAATGTCAAAACAGTA+GGG 0.598052 1_2:-32225290 Msa0067280:three_prime_UTR
GGAATTACTTCCATTAATGG+AGG 0.603430 1_2:-32225739 Msa0067280:CDS
AATTTCGGGAAGAAAGAAGA+AGG 0.603485 1_2:+32227933 None:intergenic
GGATAAAATTACAAAAGACT+AGG 0.604168 1_2:+32225416 None:intergenic
GGCGGAGGTGACTGTCTGGA+AGG 0.604378 1_2:+32227641 None:intergenic
GCGGAAGATCTGGCGACACG+TGG 0.605748 1_2:-32227347 Msa0067280:CDS
GAAGATCTGGCGACACGTGG+AGG 0.613126 1_2:-32227344 Msa0067280:CDS
TGCCGTGTAAACGGACGGTG+GGG 0.616790 1_2:+32227738 None:intergenic
GCGTGACGTGGCATTTGGAG+AGG 0.617555 1_2:-32227382 Msa0067280:CDS
GAGTGTACTAACATATGGGT+TGG 0.618574 1_2:+32228021 None:intergenic
GGACTTGATCAGCATGTGCA+GGG 0.618599 1_2:-32225586 Msa0067280:CDS
ACTCCCCATCTTCGGATCAA+CGG 0.620041 1_2:+32227313 None:intergenic
AGGCCGTTGATCCGAAGATG+GGG 0.632093 1_2:-32227316 Msa0067280:CDS
TTGATGTGTAAATGAAGTGA+AGG 0.636140 1_2:+32227978 None:intergenic
TGTGCCGTGTAAACGGACGG+TGG 0.637509 1_2:+32227736 None:intergenic
ATGAATCATGTCATGTGAAG+TGG 0.645264 1_2:+32228059 None:intergenic
TGCATTACAAAAGAAATACA+GGG 0.645577 1_2:-32225367 Msa0067280:three_prime_UTR
ACCGGCACCACCGACGACGA+CGG 0.649779 1_2:+32227476 None:intergenic
CGTCCGTTTACACGGCACAC+CGG 0.654675 1_2:-32227732 Msa0067280:CDS
CGGTGTGCCGTGTAAACGGA+CGG 0.655901 1_2:+32227733 None:intergenic
GTCCAACTATAACACTCACA+TGG 0.661124 1_2:-32225492 Msa0067280:three_prime_UTR
CTGTCAGTACCATCACTCAC+CGG 0.663216 1_2:+32227458 None:intergenic
TAGGTGAGAGCCCTTCAACA+AGG 0.668863 1_2:+32225628 None:intergenic
TTCTACGGTTTATTGACGCG+CGG 0.668910 1_2:-32227523 Msa0067280:CDS
GGAAGGTGTAACGTTGTCCG+AGG 0.670663 1_2:+32227658 None:intergenic
ACGGACGGTGGGGTAAACGG+TGG 0.672953 1_2:+32227748 None:intergenic
TGCAGCATAAGAGCGTGACG+TGG 0.673987 1_2:-32227394 Msa0067280:CDS
GATGTGATGCAAGCAACACA+TGG 0.677598 1_2:-32225666 Msa0067280:CDS
TAAACCGTAGAAGCGCGTCG+CGG 0.678232 1_2:+32227534 None:intergenic
ACCTCCGCCGTATAAAACGG+CGG 0.704052 1_2:-32227627 Msa0067280:CDS
AGGGACCCACCAACTTAGAG+GGG 0.711879 1_2:-32225271 Msa0067280:three_prime_UTR
TGATGCAAGCAACACATGGT+GGG 0.723190 1_2:-32225662 Msa0067280:CDS
CGTTGATGGTGAGAGTGACA+CGG 0.728602 1_2:-32227418 Msa0067280:CDS
GTGATGCAAGCAACACATGG+TGG 0.733444 1_2:-32225663 Msa0067280:CDS
GCATTACAAAAGAAATACAG+GGG 0.737749 1_2:-32225366 Msa0067280:three_prime_UTR
GAGTGATGGTACTGACAGCG+AGG 0.785375 1_2:-32227453 Msa0067280:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATGATAATTATATGTG+CGG - chr1_2:32226487-32226506 Msa0067280:intron 15.0%
!! TGTAAATAATCTATGTAATT+TGG + chr1_2:32226749-32226768 None:intergenic 15.0%
!!! TCTAAATTAGATAGATATTT+TGG - chr1_2:32227570-32227589 Msa0067280:CDS 15.0%
!!! CTAAATTAGATAGATATTTT+GGG - chr1_2:32227571-32227590 Msa0067280:CDS 15.0%
!! TTAGTACAGTTATTAATTAG+TGG - chr1_2:32226378-32226397 Msa0067280:intron 20.0%
!! AATAGTTCATTTCAATCTAT+GGG - chr1_2:32226680-32226699 Msa0067280:intron 20.0%
!! CATAGATTATTTACAAGAAT+AGG - chr1_2:32226754-32226773 Msa0067280:intron 20.0%
!! GTGTTTAATTAGAGTTTATT+GGG - chr1_2:32226890-32226909 Msa0067280:intron 20.0%
!!! TGATTGCATTTTAAATCATA+GGG - chr1_2:32226964-32226983 Msa0067280:intron 20.0%
!! TTTCTTGAGAATAAGTTATA+TGG - chr1_2:32227295-32227314 Msa0067280:CDS 20.0%
!! ACAAGTATATAATAACACTT+GGG - chr1_2:32227320-32227339 Msa0067280:CDS 20.0%
!!! TTTTTGTACCTTAAATCATT+TGG - chr1_2:32227349-32227368 Msa0067280:CDS 20.0%
!! ATAAAGTTCCAAATGATTTA+AGG + chr1_2:32227360-32227379 None:intergenic 20.0%
!!! GGACATATTATTTTTTTGTT+CGG - chr1_2:32227428-32227447 Msa0067280:CDS 20.0%
!!! TTTAGAATATCTAATTGTAG+TGG - chr1_2:32227459-32227478 Msa0067280:CDS 20.0%
!! ACTTCATTTATGTTCAAAAT+AGG - chr1_2:32227501-32227520 Msa0067280:CDS 20.0%
!! CAACTCAAAAGTTAAAAATT+GGG - chr1_2:32227935-32227954 Msa0067280:five_prime_UTR 20.0%
!!! AATGCTGTTTCAAAATAAAA+AGG + chr1_2:32227990-32228009 None:intergenic 20.0%
! ATATACACTAGAGAATCAAA+TGG - chr1_2:32226606-32226625 Msa0067280:intron 25.0%
! GATTAAAAGACCAATAGATT+AGG - chr1_2:32226657-32226676 Msa0067280:intron 25.0%
! ATGAACTATTCCTAATCTAT+TGG + chr1_2:32226670-32226689 None:intergenic 25.0%
! GAATAGTTCATTTCAATCTA+TGG - chr1_2:32226679-32226698 Msa0067280:intron 25.0%
! AAAAGTCTAACTTATAGCAA+TGG - chr1_2:32226867-32226886 Msa0067280:intron 25.0%
! AAAGTCTAACTTATAGCAAT+GGG - chr1_2:32226868-32226887 Msa0067280:intron 25.0%
! GGTGTTTAATTAGAGTTTAT+TGG - chr1_2:32226889-32226908 Msa0067280:intron 25.0%
! AATTAGCTAATAAGACAAGT+AGG + chr1_2:32226920-32226939 None:intergenic 25.0%
!! GTGATTGCATTTTAAATCAT+AGG - chr1_2:32226963-32226982 Msa0067280:intron 25.0%
! GACAAGTATATAATAACACT+TGG - chr1_2:32227319-32227338 Msa0067280:CDS 25.0%
! CAAGTATATAATAACACTTG+GGG - chr1_2:32227321-32227340 Msa0067280:CDS 25.0%
!!! ATTTTGGGTTCAATTTCTTT+AGG - chr1_2:32227586-32227605 Msa0067280:CDS 25.0%
!! TTTGGAATTACTTCCATTAA+TGG - chr1_2:32227640-32227659 Msa0067280:CDS 25.0%
!!! CCAATTTTTAACTTTTGAGT+TGG + chr1_2:32227937-32227956 None:intergenic 25.0%
! CCAACTCAAAAGTTAAAAAT+TGG - chr1_2:32227934-32227953 Msa0067280:five_prime_UTR 25.0%
! GGATAAAATTACAAAAGACT+AGG + chr1_2:32227969-32227988 None:intergenic 25.0%
! ATGCATTACAAAAGAAATAC+AGG - chr1_2:32228014-32228033 Msa0067280:five_prime_UTR 25.0%
! TGCATTACAAAAGAAATACA+GGG - chr1_2:32228015-32228034 Msa0067280:five_prime_UTR 25.0%
AAGTGAGTGTACTAACATAT+GGG + chr1_2:32225368-32225387 None:intergenic 30.0%
AAAGTGAGTGTACTAACATA+TGG + chr1_2:32225369-32225388 None:intergenic 30.0%
TTGATGTGTAAATGAAGTGA+AGG + chr1_2:32225407-32225426 None:intergenic 30.0%
GAAAACAAAAAACAGGGAAA+TGG + chr1_2:32225508-32225527 None:intergenic 30.0%
CGTGAAGAAAACAAAAAACA+GGG + chr1_2:32225514-32225533 None:intergenic 30.0%
ACGTGAAGAAAACAAAAAAC+AGG + chr1_2:32225515-32225534 None:intergenic 30.0%
GGATTAGATTGCAATTAATG+TGG + chr1_2:32225542-32225561 None:intergenic 30.0%
TCTAATCCTTCTCAAAGTAA+TGG - chr1_2:32225554-32225573 Msa0067280:CDS 30.0%
CTAATCCTTCTCAAAGTAAT+GGG - chr1_2:32225555-32225574 Msa0067280:CDS 30.0%
AAACTCAACTGAAAAACTCA+CGG - chr1_2:32225587-32225606 Msa0067280:CDS 30.0%
TATGTAGAGAAAATACTGTC+CGG + chr1_2:32225672-32225691 None:intergenic 30.0%
GATCAGCTCTATCAAAAATA+GGG + chr1_2:32226187-32226206 None:intergenic 30.0%
GACAAAAATGCTGAAATTGT+TGG + chr1_2:32226311-32226330 None:intergenic 30.0%
! TCTATGGGTGTAATAACTTT+TGG - chr1_2:32226695-32226714 Msa0067280:intron 30.0%
!! AGGGAGTATTGTTTAACAAT+AGG - chr1_2:32226983-32227002 Msa0067280:intron 30.0%
!! GGGAGTATTGTTTAACAATA+GGG - chr1_2:32226984-32227003 Msa0067280:intron 30.0%
AGAAATCATCAAATCTGTTG+AGG - chr1_2:32227188-32227207 Msa0067280:intron 30.0%
TACAAAGTCTCTCTGTAATT+GGG + chr1_2:32227231-32227250 None:intergenic 30.0%
TATCAAGCTCAATTCATTTC+AGG - chr1_2:32227260-32227279 Msa0067280:CDS 30.0%
! GTCTATACACATTTTGTCAA+TGG - chr1_2:32227393-32227412 Msa0067280:CDS 30.0%
! ATCTAATTGTAGTGGAGAAA+AGG - chr1_2:32227467-32227486 Msa0067280:CDS 30.0%
! TCTAATTGTAGTGGAGAAAA+GGG - chr1_2:32227468-32227487 Msa0067280:CDS 30.0%
TTGTTAAAGTTCCTTGTTGA+AGG - chr1_2:32227743-32227762 Msa0067280:CDS 30.0%
TGTTAAAGTTCCTTGTTGAA+GGG - chr1_2:32227744-32227763 Msa0067280:CDS 30.0%
ATGTACTTCATTGCATCATT+AGG + chr1_2:32227776-32227795 None:intergenic 30.0%
TAATGATGCAATGAAGTACA+TGG - chr1_2:32227775-32227794 Msa0067280:CDS 30.0%
GCATTACAAAAGAAATACAG+GGG - chr1_2:32228016-32228035 Msa0067280:five_prime_UTR 30.0%
!!! CTGTTTTGACATTCTACTTT+AGG + chr1_2:32228090-32228109 None:intergenic 30.0%
AAGTAGAATGTCAAAACAGT+AGG - chr1_2:32228091-32228110 Msa0067280:five_prime_UTR 30.0%
AGTAGAATGTCAAAACAGTA+GGG - chr1_2:32228092-32228111 Msa0067280:five_prime_UTR 30.0%
ATGAATCATGTCATGTGAAG+TGG + chr1_2:32225326-32225345 None:intergenic 35.0%
GAAGAAAGAAGAAGGATAGA+AGG + chr1_2:32225444-32225463 None:intergenic 35.0%
AATTTCGGGAAGAAAGAAGA+AGG + chr1_2:32225452-32225471 None:intergenic 35.0%
GTTAGAATCGGAATGTTGTT+GGG + chr1_2:32225484-32225503 None:intergenic 35.0%
TGTTAGAATCGGAATGTTGT+TGG + chr1_2:32225485-32225504 None:intergenic 35.0%
! AGTTTCCCATTACTTTGAGA+AGG + chr1_2:32225563-32225582 None:intergenic 35.0%
TTAATCGAATTCTCCCAGTT+GGG + chr1_2:32225795-32225814 None:intergenic 35.0%
GATTAAGGTTCCAATGAAGT+CGG - chr1_2:32225809-32225828 Msa0067280:intron 35.0%
CGATCAGCTCTATCAAAAAT+AGG + chr1_2:32226188-32226207 None:intergenic 35.0%
! ATTTTTGATAGAGCTGATCG+AGG - chr1_2:32226188-32226207 Msa0067280:CDS 35.0%
ACTAGTAGAACTAGTACTTG+TGG - chr1_2:32226336-32226355 Msa0067280:intron 35.0%
TCTTTGATGCCATTCATGAT+AGG + chr1_2:32227171-32227190 None:intergenic 35.0%
!!! TCTGTTGAGGTTTTAGATTC+TGG - chr1_2:32227201-32227220 Msa0067280:CDS 35.0%
ACAAAGTCTCTCTGTAATTG+GGG + chr1_2:32227230-32227249 None:intergenic 35.0%
GTACAAAGTCTCTCTGTAAT+TGG + chr1_2:32227232-32227251 None:intergenic 35.0%
TTGTCAATGGAAAGCAACTT+TGG - chr1_2:32227406-32227425 Msa0067280:CDS 35.0%
TGTCAATGGAAAGCAACTTT+GGG - chr1_2:32227407-32227426 Msa0067280:CDS 35.0%
ACAATGCAAACAACACTTCA+TGG - chr1_2:32227610-32227629 Msa0067280:CDS 35.0%
GGAATTACTTCCATTAATGG+AGG - chr1_2:32227643-32227662 Msa0067280:CDS 35.0%
TACTCTTCATCACATGATCA+TGG - chr1_2:32227867-32227886 Msa0067280:five_prime_UTR 35.0%
ACTCTTCATCACATGATCAT+GGG - chr1_2:32227868-32227887 Msa0067280:five_prime_UTR 35.0%
ATCCATGTGAGTGTTATAGT+TGG + chr1_2:32227895-32227914 None:intergenic 35.0%
!! AGAAATACAGGGGTATTTCA+AGG - chr1_2:32228026-32228045 Msa0067280:five_prime_UTR 35.0%
GAGTGTACTAACATATGGGT+TGG + chr1_2:32225364-32225383 None:intergenic 40.0%
GAAAGAAGAAGGATAGAAGG+TGG + chr1_2:32225441-32225460 None:intergenic 40.0%
! CAGGGAAATGGTGTTAGAAT+CGG + chr1_2:32225496-32225515 None:intergenic 40.0%
!! GGAAGCAGTATTTTGGAGAA+TGG + chr1_2:32225706-32225725 None:intergenic 40.0%
TCTCCAAAATACTGCTTCCT+CGG - chr1_2:32225707-32225726 Msa0067280:CDS 40.0%
CTTAATCGAATTCTCCCAGT+TGG + chr1_2:32225796-32225815 None:intergenic 40.0%
CAACTGGGAGAATTCGATTA+AGG - chr1_2:32225794-32225813 Msa0067280:intron 40.0%
AAGAAGCTACACTCAATTGC+TGG - chr1_2:32226118-32226137 Msa0067280:intron 40.0%
TCAATTGCTGGAAGTGATGA+TGG - chr1_2:32226130-32226149 Msa0067280:CDS 40.0%
ACACTAGAGAATCAAATGGC+TGG - chr1_2:32226610-32226629 Msa0067280:intron 40.0%
CACTAGAGAATCAAATGGCT+GGG - chr1_2:32226611-32226630 Msa0067280:intron 40.0%
! AAATGGCTGGGTTGAGTTTA+TGG - chr1_2:32226623-32226642 Msa0067280:intron 40.0%
ACAGAGTTAATGTGAGTGCA+GGG - chr1_2:32227106-32227125 Msa0067280:intron 40.0%
TGATGCCATTCATGATAGGT+AGG + chr1_2:32227167-32227186 None:intergenic 40.0%
CAAACAACACTTCATGGAAG+AGG - chr1_2:32227616-32227635 Msa0067280:CDS 40.0%
ACACTTCATGGAAGAGGATT+TGG - chr1_2:32227622-32227641 Msa0067280:CDS 40.0%
GTCCAACTATAACACTCACA+TGG - chr1_2:32227890-32227909 Msa0067280:five_prime_UTR 40.0%
TTGGGTGTGGAAGCAATTTC+GGG + chr1_2:32225466-32225485 None:intergenic 45.0%
GTTGGGTGTGGAAGCAATTT+CGG + chr1_2:32225467-32225486 None:intergenic 45.0%
AATCGGAATGTTGTTGGGTG+TGG + chr1_2:32225479-32225498 None:intergenic 45.0%
CTCAACTGAAAAACTCACGG+CGG - chr1_2:32225590-32225609 Msa0067280:CDS 45.0%
! TGTCCGAGGAAGCAGTATTT+TGG + chr1_2:32225713-32225732 None:intergenic 45.0%
TTCTACGGTTTATTGACGCG+CGG - chr1_2:32225859-32225878 Msa0067280:intron 45.0%
!! ATTTGGAGAGGTTGGTGAGA+TGG - chr1_2:32226012-32226031 Msa0067280:intron 45.0%
!! TTTGGAGAGGTTGGTGAGAT+GGG - chr1_2:32226013-32226032 Msa0067280:intron 45.0%
ATCTTCCAACTCCCCATCTT+CGG + chr1_2:32226080-32226099 None:intergenic 45.0%
!! GATGATGGAGCATGCTCAAA+TGG - chr1_2:32226145-32226164 Msa0067280:CDS 45.0%
! GATCGGGATTCGAGTTTGAA+TGG + chr1_2:32226276-32226295 None:intergenic 45.0%
TCAAACTCGAATCCCGATCA+TGG - chr1_2:32226277-32226296 Msa0067280:intron 45.0%
TGCTCGATAGCTATGAAGCA+CGG - chr1_2:32226403-32226422 Msa0067280:intron 45.0%
ATAGCTATGAAGCACGGATG+CGG - chr1_2:32226409-32226428 Msa0067280:intron 45.0%
GACACTGGAATAGTAATCCG+AGG - chr1_2:32226526-32226545 Msa0067280:intron 45.0%
TACTTCGCAACTGTGAAGCT+CGG + chr1_2:32226557-32226576 None:intergenic 45.0%
CACAGAGTTAATGTGAGTGC+AGG - chr1_2:32227105-32227124 Msa0067280:intron 45.0%
TGCAAAGGAGGAACATGCTA+GGG - chr1_2:32227140-32227159 Msa0067280:intron 45.0%
AGGGACCTACCTATCATGAA+TGG - chr1_2:32227159-32227178 Msa0067280:intron 45.0%
ACTTCCATTAATGGAGGCAG+TGG - chr1_2:32227649-32227668 Msa0067280:CDS 45.0%
AACTCCACTGCCTCCATTAA+TGG + chr1_2:32227656-32227675 None:intergenic 45.0%
GATGTGATGCAAGCAACACA+TGG - chr1_2:32227716-32227735 Msa0067280:CDS 45.0%
TGATGCAAGCAACACATGGT+GGG - chr1_2:32227720-32227739 Msa0067280:CDS 45.0%
CTATTTGAGCCCCTCTAAGT+TGG + chr1_2:32228123-32228142 None:intergenic 45.0%
CAACTTAGAGGGGCTCAAAT+AGG - chr1_2:32228121-32228140 Msa0067280:five_prime_UTR 45.0%
! GCGGAGATTTTACCCAACGA+CGG - chr1_2:32225609-32225628 Msa0067280:CDS 50.0%
TAAACGGACGGTGGGGTAAA+CGG + chr1_2:32225640-32225659 None:intergenic 50.0%
GTCACCTCCGCCGTATAAAA+CGG - chr1_2:32225752-32225771 Msa0067280:CDS 50.0%
GACTCGAAGTCCGACTTCAT+TGG + chr1_2:32225822-32225841 None:intergenic 50.0%
ATGAAGTCGGACTTCGAGTC+AGG - chr1_2:32225822-32225841 Msa0067280:intron 50.0%
GTTTATTGACGCGCGGTTTC+CGG - chr1_2:32225866-32225885 Msa0067280:intron 50.0%
CTGTCAGTACCATCACTCAC+CGG + chr1_2:32225927-32225946 None:intergenic 50.0%
!! CGTTGATGGTGAGAGTGACA+CGG - chr1_2:32225964-32225983 Msa0067280:intron 50.0%
! TAAGAGCGTGACGTGGCATT+TGG - chr1_2:32225995-32226014 Msa0067280:intron 50.0%
AAGGCCGTTGATCCGAAGAT+GGG - chr1_2:32226065-32226084 Msa0067280:intron 50.0%
ACTCCCCATCTTCGGATCAA+CGG + chr1_2:32226072-32226091 None:intergenic 50.0%
TTGATCCGAAGATGGGGAGT+TGG - chr1_2:32226072-32226091 Msa0067280:intron 50.0%
GAAGATGGGGAGTTGGAAGA+TGG - chr1_2:32226079-32226098 Msa0067280:intron 50.0%
TTGGACTCAACGCCATGATC+GGG + chr1_2:32226292-32226311 None:intergenic 50.0%
GTTGGACTCAACGCCATGAT+CGG + chr1_2:32226293-32226312 None:intergenic 50.0%
TGTGAGTGCAGGGCTATCTA+AGG - chr1_2:32227116-32227135 Msa0067280:intron 50.0%
! AGGGCTATCTAAGGCTGCAA+AGG - chr1_2:32227125-32227144 Msa0067280:intron 50.0%
! GCTATCTAAGGCTGCAAAGG+AGG - chr1_2:32227128-32227147 Msa0067280:intron 50.0%
CTGCAAAGGAGGAACATGCT+AGG - chr1_2:32227139-32227158 Msa0067280:intron 50.0%
GTGATGCAAGCAACACATGG+TGG - chr1_2:32227719-32227738 Msa0067280:CDS 50.0%
TAGGTGAGAGCCCTTCAACA+AGG + chr1_2:32227757-32227776 None:intergenic 50.0%
TGGACTTGATCAGCATGTGC+AGG - chr1_2:32227795-32227814 Msa0067280:CDS 50.0%
GGACTTGATCAGCATGTGCA+GGG - chr1_2:32227796-32227815 Msa0067280:CDS 50.0%
GTGCAGGGACAGAGAGAAAA+TGG - chr1_2:32227811-32227830 Msa0067280:CDS 50.0%
TAGGGACCCACCAACTTAGA+GGG - chr1_2:32228110-32228129 Msa0067280:five_prime_UTR 50.0%
!! TTGAGCCCCTCTAAGTTGGT+GGG + chr1_2:32228119-32228138 None:intergenic 50.0%
! TTTGAGCCCCTCTAAGTTGG+TGG + chr1_2:32228120-32228139 None:intergenic 50.0%
TAAACGGTGGCTCCGTCGTT+GGG + chr1_2:32225624-32225643 None:intergenic 55.0%
TACCCCACCGTCCGTTTACA+CGG - chr1_2:32225642-32225661 Msa0067280:CDS 55.0%
TGTCCGGTGTGCCGTGTAAA+CGG + chr1_2:32225656-32225675 None:intergenic 55.0%
! GGAAGGTGTAACGTTGTCCG+AGG + chr1_2:32225727-32225746 None:intergenic 55.0%
ACCTCCGCCGTATAAAACGG+CGG - chr1_2:32225755-32225774 Msa0067280:CDS 55.0%
! TAAACCGTAGAAGCGCGTCG+CGG + chr1_2:32225851-32225870 None:intergenic 55.0%
!! GAGTGATGGTACTGACAGCG+AGG - chr1_2:32225929-32225948 Msa0067280:intron 55.0%
GGATGCAACGACGTCGTTGA+TGG - chr1_2:32225950-32225969 Msa0067280:intron 55.0%
! TGCAGCATAAGAGCGTGACG+TGG - chr1_2:32225988-32226007 Msa0067280:intron 55.0%
GACGTGGCATTTGGAGAGGT+TGG - chr1_2:32226004-32226023 Msa0067280:intron 55.0%
AAGATCTGGCGACACGTGGA+GGG - chr1_2:32226039-32226058 Msa0067280:intron 55.0%
GAAGGCCGTTGATCCGAAGA+TGG - chr1_2:32226064-32226083 Msa0067280:intron 55.0%
AGGCCGTTGATCCGAAGATG+GGG - chr1_2:32226066-32226085 Msa0067280:intron 55.0%
GATGGGGAGTTGGAAGATGG+AGG - chr1_2:32226082-32226101 Msa0067280:intron 55.0%
ATGAAGCACGGATGCGGACA+TGG - chr1_2:32226415-32226434 Msa0067280:intron 55.0%
TGTGAAGCTCGGACACTCCT+CGG + chr1_2:32226546-32226565 None:intergenic 55.0%
GTAGGGACCCACCAACTTAG+AGG - chr1_2:32228109-32228128 Msa0067280:five_prime_UTR 55.0%
AGGGACCCACCAACTTAGAG+GGG - chr1_2:32228111-32228130 Msa0067280:five_prime_UTR 55.0%
GTAAACGGTGGCTCCGTCGT+TGG + chr1_2:32225625-32225644 None:intergenic 60.0%
TGCCGTGTAAACGGACGGTG+GGG + chr1_2:32225647-32225666 None:intergenic 60.0%
GTGCCGTGTAAACGGACGGT+GGG + chr1_2:32225648-32225667 None:intergenic 60.0%
TGTGCCGTGTAAACGGACGG+TGG + chr1_2:32225649-32225668 None:intergenic 60.0%
CGGTGTGCCGTGTAAACGGA+CGG + chr1_2:32225652-32225671 None:intergenic 60.0%
CGTCCGTTTACACGGCACAC+CGG - chr1_2:32225650-32225669 Msa0067280:CDS 60.0%
ATACGGCGGAGGTGACTGTC+TGG + chr1_2:32225748-32225767 None:intergenic 60.0%
!! CCACGCGCCGCCGTTTTATA+CGG + chr1_2:32225765-32225784 None:intergenic 60.0%
CGTATAAAACGGCGGCGCGT+GGG - chr1_2:32225763-32225782 Msa0067280:CDS 60.0%
! GCGTGGGTTGCAACCCAACT+GGG - chr1_2:32225779-32225798 Msa0067280:intron 60.0%
! AAACCGTAGAAGCGCGTCGC+GGG + chr1_2:32225850-32225869 None:intergenic 60.0%
GCGTGACGTGGCATTTGGAG+AGG - chr1_2:32226000-32226019 Msa0067280:intron 60.0%
!! GGAGAGGTTGGTGAGATGGG+CGG - chr1_2:32226016-32226035 Msa0067280:intron 60.0%
GGTGAGATGGGCGGAAGATC+TGG - chr1_2:32226025-32226044 Msa0067280:intron 60.0%
GAAGATCTGGCGACACGTGG+AGG - chr1_2:32226038-32226057 Msa0067280:intron 60.0%
!! GTGCGGTGTCGTGTCGATGT+CGG - chr1_2:32226504-32226523 Msa0067280:intron 60.0%
! GTCGTGTCGATGTCGGACAC+TGG - chr1_2:32226511-32226530 Msa0067280:intron 60.0%
ACGGACGGTGGGGTAAACGG+TGG + chr1_2:32225637-32225656 None:intergenic 65.0%
GGCGGAGGTGACTGTCTGGA+AGG + chr1_2:32225744-32225763 None:intergenic 65.0%
!! GCCGCCGTTTTATACGGCGG+AGG + chr1_2:32225759-32225778 None:intergenic 65.0%
!! CGCGCCGCCGTTTTATACGG+CGG + chr1_2:32225762-32225781 None:intergenic 65.0%
CCGTATAAAACGGCGGCGCG+TGG - chr1_2:32225762-32225781 Msa0067280:CDS 65.0%
! CGCGTGGGTTGCAACCCAAC+TGG - chr1_2:32225778-32225797 Msa0067280:intron 65.0%
! GTTTCCGGAGCTGTCGGCTG+AGG - chr1_2:32225881-32225900 Msa0067280:intron 65.0%
GGTTCCTCAGCCGACAGCTC+CGG + chr1_2:32225888-32225907 None:intergenic 65.0%
GGCTGAGGAACCGTCGTCGT+CGG - chr1_2:32225896-32225915 Msa0067280:intron 65.0%
TGAGGAACCGTCGTCGTCGG+TGG - chr1_2:32225899-32225918 Msa0067280:intron 65.0%
!! TCGGTGGTGCCGGTGAGTGA+TGG - chr1_2:32225915-32225934 Msa0067280:intron 65.0%
GCGGAAGATCTGGCGACACG+TGG - chr1_2:32226035-32226054 Msa0067280:intron 65.0%
ATCTGGCGACACGTGGAGGG+AGG - chr1_2:32226042-32226061 Msa0067280:intron 65.0%
GCTCCCGCGACGCGCTTCTA+CGG - chr1_2:32225844-32225863 Msa0067280:intron 70.0%
CGCGCGGTTTCCGGAGCTGT+CGG - chr1_2:32225875-32225894 Msa0067280:intron 70.0%
ACCGTCGTCGTCGGTGGTGC+CGG - chr1_2:32225905-32225924 Msa0067280:intron 70.0%
ACCGGCACCACCGACGACGA+CGG + chr1_2:32225909-32225928 None:intergenic 70.0%
GGCGACACGTGGAGGGAGGA+AGG - chr1_2:32226046-32226065 Msa0067280:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 32225244 32228160 32225244 ID=Msa0067280;Name=Msa0067280
chr1_2 mRNA 32225244 32228160 32225244 ID=Msa0067280-mRNA-1;Parent=Msa0067280;Name=Msa0067280-mRNA-1;_AED=0.00;_eAED=0.00;_QI=329|1|1|1|1|1|3|298|348
chr1_2 exon 32225244 32225796 32225244 ID=Msa0067280-mRNA-1:exon:10558;Parent=Msa0067280-mRNA-1
chr1_2 exon 32226123 32226277 32226123 ID=Msa0067280-mRNA-1:exon:10557;Parent=Msa0067280-mRNA-1
chr1_2 exon 32227195 32228160 32227195 ID=Msa0067280-mRNA-1:exon:10556;Parent=Msa0067280-mRNA-1
chr1_2 five_prime_UTR 32227832 32228160 32227832 ID=Msa0067280-mRNA-1:five_prime_utr;Parent=Msa0067280-mRNA-1
chr1_2 CDS 32227195 32227831 32227195 ID=Msa0067280-mRNA-1:cds;Parent=Msa0067280-mRNA-1
chr1_2 CDS 32226123 32226277 32226123 ID=Msa0067280-mRNA-1:cds;Parent=Msa0067280-mRNA-1
chr1_2 CDS 32225542 32225796 32225542 ID=Msa0067280-mRNA-1:cds;Parent=Msa0067280-mRNA-1
chr1_2 three_prime_UTR 32225244 32225541 32225244 ID=Msa0067280-mRNA-1:three_prime_utr;Parent=Msa0067280-mRNA-1
Gene Sequence

>Msa0067280

ATGGGAAACTGTATAAACTCAACTGAAAAACTCACGGCGGAGATTTTACCCAACGACGGAGCCACCGTTTACCCCACCGTCCGTTTACACGGCACACCGGACAGTATTTTCTCTACATACATTCGCTTCGCCATTCTCCAAAATACTGCTTCCTCGGACAACGTTACACCTTCCAGACAGTCACCTCCGCCGTATAAAACGGCGGCGCGTGGGTTGCAACCCAACTGGGAGAATTCGATTAAGGTTCCAATGAAGTCGGACTTCGAGTCAGGCTCCCGCGACGCGCTTCTACGGTTTATTGACGCGCGGTTTCCGGAGCTGTCGGCTGAGGAACCGTCGTCGTCGGTGGTGCCGGTGAGTGATGGTACTGACAGCGAGGATGCAACGACGTCGTTGATGGTGAGAGTGACACGGCTGCAGCATAAGAGCGTGACGTGGCATTTGGAGAGGTTGGTGAGATGGGCGGAAGATCTGGCGACACGTGGAGGGAGGAAGGCCGTTGATCCGAAGATGGGGAGTTGGAAGATGGAGGTGAGTAAATTTGCAAGAAGCTACACTCAATTGCTGGAAGTGATGATGGAGCATGCTCAAATGGAAGAGAGAGTTCTCTTCCCTATTTTTGATAGAGCTGATCGAGGGCTATCTAAGGCTGCAAAGGAGGAACATGCTAGGGACCTACCTATCATGAATGGCATCAAAGAAATCATCAAATCTGTTGAGGTTTTAGATTCTGGATGCCCCAATTACAGAGAGACTTTGTACAATCTGTCTATCAAGCTCAATTCATTTCAGGAACAATGCAAACAACACTTCATGGAAGAGGATTTGGAATTACTTCCATTAATGGAGGCAGTGGAGTTGAGCAAAGAGCAAGATGAGAGAGCACTCGAACAATGTTTTGATGTGATGCAAGCAACACATGGTGGGTTGTTAAAGTTCCTTGTTGAAGGGCTCTCACCTAATGATGCAATGAAGTACATGGACTTGATCAGCATGTGCAGGGACAGAGAGAAAATGGAATCAATGCTACGAATGATGATTGAATGA
Protein sequence

>Msa0067280

MGNCINSTEKLTAEILPNDGATVYPTVRLHGTPDSIFSTYIRFAILQNTASSDNVTPSRQSPPPYKTAARGLQPNWENSIKVPMKSDFESGSRDALLRFIDARFPELSAEEPSSSVVPVSDGTDSEDATTSLMVRVTRLQHKSVTWHLERLVRWAEDLATRGGRKAVDPKMGSWKMEVSKFARSYTQLLEVMMEHAQMEERVLFPIFDRADRGLSKAAKEEHARDLPIMNGIKEIIKSVEVLDSGCPNYRETLYNLSIKLNSFQEQCKQHFMEEDLELLPLMEAVELSKEQDERALEQCFDVMQATHGGLLKFLVEGLSPNDAMKYMDLISMCRDREKMESMLRMMIE*