AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0085020

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085020 MtrunA17_Chr1g0200861 66.055 218 7 3 5 214 32 190 1.86e-88 258
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085020 AT1G71865.1 65.306 98 29 4 116 211 1 95 9.08e-28 101

Find 47 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTATAAGGTTCAAAATTT+TGG 0.124660 1_2:+64866653 Msa0085020:CDS
TTGCTTTGCCATTGTTCTTT+TGG 0.201399 1_2:+64867684 Msa0085020:three_prime_UTR
TGTCACCATCACACTCTTTC+AGG 0.206894 1_2:+64867216 Msa0085020:CDS
ATAGATAGGTAGAGATTATA+AGG 0.301627 1_2:+64866640 Msa0085020:intron
TTGTAACTTTATAACCATAC+TGG 0.412519 1_2:-64866697 None:intergenic
AATCAGTGAAATTGAAAAGA+AGG 0.422241 1_2:-64865645 None:intergenic
GCATTTCCGATACACCAGTA+TGG 0.430298 1_2:+64866683 Msa0085020:CDS
TCTATCAAGGTTAATTATGA+AGG 0.433912 1_2:+64867751 Msa0085020:three_prime_UTR
AAGAACAATGGCAAAGCAAA+TGG 0.453315 1_2:-64867680 None:intergenic
CCAGGTATCTCAGTGTTACT+AGG 0.459360 1_2:-64867371 None:intergenic
ATCTCAGTGTTACTAGGTTG+AGG 0.466517 1_2:-64867365 None:intergenic
TTATAAAGTTACAATGTTTG+AGG 0.479171 1_2:+64866706 Msa0085020:CDS
TAATATCTAGTTATCTATCA+AGG 0.483873 1_2:+64867738 Msa0085020:three_prime_UTR
GGAGGGACATAGTACAAGAT+TGG 0.483942 1_2:+64867426 Msa0085020:CDS
CTCTTCTCTTCTCTCAAACG+AGG 0.511025 1_2:+64865562 Msa0085020:CDS
AACGTGCTGGAGAAAGACTA+AGG 0.512886 1_2:+64867481 Msa0085020:three_prime_UTR
TCTCAGTGTTACTAGGTTGA+GGG 0.523772 1_2:-64867364 None:intergenic
ATCAGTGAAATTGAAAAGAA+GGG 0.524601 1_2:-64865644 None:intergenic
ACTAGGTTGAGGGGTAACTT+TGG 0.527701 1_2:-64867354 None:intergenic
CTTACAAGCCGCGGAGGACC+AGG 0.527990 1_2:-64867389 None:intergenic
GCAAGAAAAGAGTGTACAGT+AGG 0.530811 1_2:-64867320 None:intergenic
TTATAACCATACTGGTGTAT+CGG 0.531699 1_2:-64866689 None:intergenic
GAGCAGAGAAAGTGTGTGAG+TGG 0.540683 1_2:-64865542 None:intergenic
AGCAGAGAAAGTGTGTGAGT+GGG 0.553074 1_2:-64865541 None:intergenic
CCGCTTAGTCAACAACGTGC+TGG 0.555117 1_2:+64867468 Msa0085020:exon
CAAGAAAAGAGTGTACAGTA+GGG 0.557860 1_2:-64867319 None:intergenic
AAGAAGTCGTGCCGTGAGGA+GGG 0.581217 1_2:+64867409 Msa0085020:CDS
TAAGAAGTCGTGCCGTGAGG+AGG 0.581437 1_2:+64867408 Msa0085020:CDS
CCTAGTAACACTGAGATACC+TGG 0.585674 1_2:+64867371 Msa0085020:CDS
AAGAAAAGAGTGTACAGTAG+GGG 0.587683 1_2:-64867318 None:intergenic
TTGTACTATGTCCCTCCTCA+CGG 0.590509 1_2:-64867420 None:intergenic
CTCAGTGTTACTAGGTTGAG+GGG 0.603718 1_2:-64867363 None:intergenic
AAAACACCACACAACAACAG+TGG 0.605311 1_2:+64865472 Msa0085020:CDS
CCAGCACGTTGTTGACTAAG+CGG 0.627407 1_2:-64867468 None:intergenic
TTGCAGGGGAAGACTGTGAG+AGG 0.629182 1_2:+64867149 Msa0085020:intron
CTATTGCAAACTGCTTAACA+CGG 0.633983 1_2:+64867279 Msa0085020:CDS
AGAAAAGAGTGTACAGTAGG+GGG 0.639401 1_2:-64867317 None:intergenic
CGACTTCTTACAAGCCGCGG+AGG 0.642258 1_2:-64867395 None:intergenic
ATCGTGAACCAAAAGAACAA+TGG 0.643285 1_2:-64867692 None:intergenic
ACTTGGCCACTGTTGTTGTG+TGG 0.646918 1_2:-64865478 None:intergenic
ACGTGCTGGAGAAAGACTAA+GGG 0.647434 1_2:+64867482 Msa0085020:three_prime_UTR
GCACGACTTCTTACAAGCCG+CGG 0.650729 1_2:-64867398 None:intergenic
GTTTCGAATTCAGATAACGA+AGG 0.669866 1_2:+64867245 Msa0085020:CDS
ATGTCTTGTGTGCCATAGTG+TGG 0.670039 1_2:+64867187 Msa0085020:CDS
TTGTAAGAAGTCGTGCCGTG+AGG 0.694116 1_2:+64867405 Msa0085020:CDS
GATCTCCTGAAAGAGTGTGA+TGG 0.713714 1_2:-64867221 None:intergenic
CTGAGATACCTGGTCCTCCG+CGG 0.727962 1_2:+64867381 Msa0085020:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAATCATTTTAATCTAATTT+TGG + chr1_2:64866059-64866078 Msa0085020:intron 15.0%
!!! ACAAAATTTTTATTCTTTAG+TGG + chr1_2:64866271-64866290 Msa0085020:intron 15.0%
!!! TTTTTCTTAGAAGTTAAAAT+TGG - chr1_2:64866514-64866533 None:intergenic 15.0%
!!! CAATCATTTTAATCTAATTT+TGG + chr1_2:64866059-64866078 Msa0085020:intron 15.0%
!!! ACAAAATTTTTATTCTTTAG+TGG + chr1_2:64866271-64866290 Msa0085020:intron 15.0%
!!! TTTTTCTTAGAAGTTAAAAT+TGG - chr1_2:64866514-64866533 None:intergenic 15.0%
!! AAGCAGAAAGAAAAAAAAAA+CGG - chr1_2:64865605-64865624 None:intergenic 20.0%
!!! TTTTCAATTTCACTGATTTT+GGG + chr1_2:64865650-64865669 Msa0085020:CDS 20.0%
!! ATCTCATTTATGTTATTCAT+TGG + chr1_2:64865682-64865701 Msa0085020:intron 20.0%
!! AGCAATAAGGTAAAATATTA+CGG - chr1_2:64865829-64865848 None:intergenic 20.0%
!!! AATATTTTACCTTATTGCTA+TGG + chr1_2:64865830-64865849 Msa0085020:intron 20.0%
!! TAAAGTATACCATAAGTATA+GGG - chr1_2:64866586-64866605 None:intergenic 20.0%
!!! GATTATAAGGTTCAAAATTT+TGG + chr1_2:64866653-64866672 Msa0085020:CDS 20.0%
!! TTATAAAGTTACAATGTTTG+AGG + chr1_2:64866706-64866725 Msa0085020:CDS 20.0%
!! GGAAATAACATATAAAGAAT+GGG - chr1_2:64867036-64867055 None:intergenic 20.0%
!!! AGTAGTTTTTCTCTAATTTT+GGG + chr1_2:64867518-64867537 Msa0085020:three_prime_UTR 20.0%
!! TAATATCTAGTTATCTATCA+AGG + chr1_2:64867738-64867757 Msa0085020:three_prime_UTR 20.0%
!! AAGCAGAAAGAAAAAAAAAA+CGG - chr1_2:64865605-64865624 None:intergenic 20.0%
!!! TTTTCAATTTCACTGATTTT+GGG + chr1_2:64865650-64865669 Msa0085020:CDS 20.0%
!! ATCTCATTTATGTTATTCAT+TGG + chr1_2:64865682-64865701 Msa0085020:intron 20.0%
!! AGCAATAAGGTAAAATATTA+CGG - chr1_2:64865829-64865848 None:intergenic 20.0%
!!! AATATTTTACCTTATTGCTA+TGG + chr1_2:64865830-64865849 Msa0085020:intron 20.0%
!! TAAAGTATACCATAAGTATA+GGG - chr1_2:64866586-64866605 None:intergenic 20.0%
!!! GATTATAAGGTTCAAAATTT+TGG + chr1_2:64866653-64866672 Msa0085020:CDS 20.0%
!! TTATAAAGTTACAATGTTTG+AGG + chr1_2:64866706-64866725 Msa0085020:CDS 20.0%
!! GGAAATAACATATAAAGAAT+GGG - chr1_2:64867036-64867055 None:intergenic 20.0%
!!! AGTAGTTTTTCTCTAATTTT+GGG + chr1_2:64867518-64867537 Msa0085020:three_prime_UTR 20.0%
!! TAATATCTAGTTATCTATCA+AGG + chr1_2:64867738-64867757 Msa0085020:three_prime_UTR 20.0%
!! ATTTGATAAGATCTTTTACG+AGG - chr1_2:64865513-64865532 None:intergenic 25.0%
! AAAAAAAAAACGGAATGAGA+GGG - chr1_2:64865595-64865614 None:intergenic 25.0%
! ATCAGTGAAATTGAAAAGAA+GGG - chr1_2:64865647-64865666 None:intergenic 25.0%
! AATCAGTGAAATTGAAAAGA+AGG - chr1_2:64865648-64865667 None:intergenic 25.0%
!!! CTTTTCAATTTCACTGATTT+TGG + chr1_2:64865649-64865668 Msa0085020:CDS 25.0%
! ATGATTTCAAATCTTGCAAT+GGG + chr1_2:64865763-64865782 Msa0085020:intron 25.0%
! AAATTGTTTCCATAGCAATA+AGG - chr1_2:64865842-64865861 None:intergenic 25.0%
!!! CAATTTCAATTTTCAAGTCA+AGG + chr1_2:64865856-64865875 Msa0085020:intron 25.0%
!! TTTTGTGAATGAATACTAAG+TGG + chr1_2:64865881-64865900 Msa0085020:intron 25.0%
! TTTGTGAATGAATACTAAGT+GGG + chr1_2:64865882-64865901 Msa0085020:intron 25.0%
! ATTATAATCTGCTTGTGTTT+AGG + chr1_2:64865992-64866011 Msa0085020:intron 25.0%
! CTTTAGTGGTATATGTAAAA+TGG + chr1_2:64866285-64866304 Msa0085020:intron 25.0%
! CTTAGAAGTTAAAATTGGAT+TGG - chr1_2:64866509-64866528 None:intergenic 25.0%
! CTAAAGTATACCATAAGTAT+AGG - chr1_2:64866587-64866606 None:intergenic 25.0%
! GCTTAACATGAATAATAGAT+AGG + chr1_2:64866626-64866645 Msa0085020:intron 25.0%
! ATAGATAGGTAGAGATTATA+AGG + chr1_2:64866640-64866659 Msa0085020:intron 25.0%
! TTGTAACTTTATAACCATAC+TGG - chr1_2:64866700-64866719 None:intergenic 25.0%
! CTTGGTAAGCTAAAAAAAAA+GGG - chr1_2:64866956-64866975 None:intergenic 25.0%
! ACTTGGTAAGCTAAAAAAAA+AGG - chr1_2:64866957-64866976 None:intergenic 25.0%
! GGGAAATAACATATAAAGAA+TGG - chr1_2:64867037-64867056 None:intergenic 25.0%
!! CAGTAGTTTTTCTCTAATTT+TGG + chr1_2:64867517-64867536 Msa0085020:three_prime_UTR 25.0%
!! GTAGTTTTTCTCTAATTTTG+GGG + chr1_2:64867519-64867538 Msa0085020:three_prime_UTR 25.0%
!! TAGTTTTTCTCTAATTTTGG+GGG + chr1_2:64867520-64867539 Msa0085020:three_prime_UTR 25.0%
! TCTATCAAGGTTAATTATGA+AGG + chr1_2:64867751-64867770 Msa0085020:three_prime_UTR 25.0%
!! ATTTGATAAGATCTTTTACG+AGG - chr1_2:64865513-64865532 None:intergenic 25.0%
! AAAAAAAAAACGGAATGAGA+GGG - chr1_2:64865595-64865614 None:intergenic 25.0%
! ATCAGTGAAATTGAAAAGAA+GGG - chr1_2:64865647-64865666 None:intergenic 25.0%
! AATCAGTGAAATTGAAAAGA+AGG - chr1_2:64865648-64865667 None:intergenic 25.0%
!!! CTTTTCAATTTCACTGATTT+TGG + chr1_2:64865649-64865668 Msa0085020:CDS 25.0%
! ATGATTTCAAATCTTGCAAT+GGG + chr1_2:64865763-64865782 Msa0085020:intron 25.0%
! AAATTGTTTCCATAGCAATA+AGG - chr1_2:64865842-64865861 None:intergenic 25.0%
!!! CAATTTCAATTTTCAAGTCA+AGG + chr1_2:64865856-64865875 Msa0085020:intron 25.0%
!! TTTTGTGAATGAATACTAAG+TGG + chr1_2:64865881-64865900 Msa0085020:intron 25.0%
! TTTGTGAATGAATACTAAGT+GGG + chr1_2:64865882-64865901 Msa0085020:intron 25.0%
! ATTATAATCTGCTTGTGTTT+AGG + chr1_2:64865992-64866011 Msa0085020:intron 25.0%
! CTTTAGTGGTATATGTAAAA+TGG + chr1_2:64866285-64866304 Msa0085020:intron 25.0%
! CTTAGAAGTTAAAATTGGAT+TGG - chr1_2:64866509-64866528 None:intergenic 25.0%
! CTAAAGTATACCATAAGTAT+AGG - chr1_2:64866587-64866606 None:intergenic 25.0%
! GCTTAACATGAATAATAGAT+AGG + chr1_2:64866626-64866645 Msa0085020:intron 25.0%
! ATAGATAGGTAGAGATTATA+AGG + chr1_2:64866640-64866659 Msa0085020:intron 25.0%
! TTGTAACTTTATAACCATAC+TGG - chr1_2:64866700-64866719 None:intergenic 25.0%
! CTTGGTAAGCTAAAAAAAAA+GGG - chr1_2:64866956-64866975 None:intergenic 25.0%
! ACTTGGTAAGCTAAAAAAAA+AGG - chr1_2:64866957-64866976 None:intergenic 25.0%
! GGGAAATAACATATAAAGAA+TGG - chr1_2:64867037-64867056 None:intergenic 25.0%
!! CAGTAGTTTTTCTCTAATTT+TGG + chr1_2:64867517-64867536 Msa0085020:three_prime_UTR 25.0%
!! GTAGTTTTTCTCTAATTTTG+GGG + chr1_2:64867519-64867538 Msa0085020:three_prime_UTR 25.0%
!! TAGTTTTTCTCTAATTTTGG+GGG + chr1_2:64867520-64867539 Msa0085020:three_prime_UTR 25.0%
! TCTATCAAGGTTAATTATGA+AGG + chr1_2:64867751-64867770 Msa0085020:three_prime_UTR 25.0%
GAAAAAAAAAACGGAATGAG+AGG - chr1_2:64865596-64865615 None:intergenic 30.0%
! TGCAAGATTTGAAATCATCA+TGG - chr1_2:64865761-64865780 None:intergenic 30.0%
GATGATTTCAAATCTTGCAA+TGG + chr1_2:64865762-64865781 Msa0085020:intron 30.0%
TTGTGAATGAATACTAAGTG+GGG + chr1_2:64865883-64865902 Msa0085020:intron 30.0%
ATGATTGATTATCTAGCATG+CGG - chr1_2:64866046-64866065 None:intergenic 30.0%
ACAAGGTTAATCATTCTCAA+AGG - chr1_2:64866101-64866120 None:intergenic 30.0%
AAGAAGCAACATACAAAGTT+TGG - chr1_2:64866136-64866155 None:intergenic 30.0%
!! GCTATGCTCTTTAAAGTATT+TGG + chr1_2:64866434-64866453 Msa0085020:intron 30.0%
! TTTTTCTGTCCCTATACTTA+TGG + chr1_2:64866574-64866593 Msa0085020:intron 30.0%
! ACTTTAGTGTTTGAAACATG+TGG + chr1_2:64866600-64866619 Msa0085020:intron 30.0%
!!! GGTTCAAAATTTTGGTAATG+TGG + chr1_2:64866661-64866680 Msa0085020:CDS 30.0%
TTATAACCATACTGGTGTAT+CGG - chr1_2:64866692-64866711 None:intergenic 30.0%
CATAAGAAATGAATGAGTTG+TGG + chr1_2:64866787-64866806 Msa0085020:intron 30.0%
AATGAATGAGTTGTGGTATA+AGG + chr1_2:64866794-64866813 Msa0085020:intron 30.0%
!!! TATGTTATTTCCCACACTTT+TGG + chr1_2:64867044-64867063 Msa0085020:intron 30.0%
TAATTTCTGACATGAATTGC+AGG + chr1_2:64867133-64867152 Msa0085020:intron 30.0%
AATTTCTGACATGAATTGCA+GGG + chr1_2:64867134-64867153 Msa0085020:intron 30.0%
GAAAAAAAAAACGGAATGAG+AGG - chr1_2:64865596-64865615 None:intergenic 30.0%
! TGCAAGATTTGAAATCATCA+TGG - chr1_2:64865761-64865780 None:intergenic 30.0%
GATGATTTCAAATCTTGCAA+TGG + chr1_2:64865762-64865781 Msa0085020:intron 30.0%
TTGTGAATGAATACTAAGTG+GGG + chr1_2:64865883-64865902 Msa0085020:intron 30.0%
ATGATTGATTATCTAGCATG+CGG - chr1_2:64866046-64866065 None:intergenic 30.0%
ACAAGGTTAATCATTCTCAA+AGG - chr1_2:64866101-64866120 None:intergenic 30.0%
AAGAAGCAACATACAAAGTT+TGG - chr1_2:64866136-64866155 None:intergenic 30.0%
!! GCTATGCTCTTTAAAGTATT+TGG + chr1_2:64866434-64866453 Msa0085020:intron 30.0%
! TTTTTCTGTCCCTATACTTA+TGG + chr1_2:64866574-64866593 Msa0085020:intron 30.0%
! ACTTTAGTGTTTGAAACATG+TGG + chr1_2:64866600-64866619 Msa0085020:intron 30.0%
!!! GGTTCAAAATTTTGGTAATG+TGG + chr1_2:64866661-64866680 Msa0085020:CDS 30.0%
TTATAACCATACTGGTGTAT+CGG - chr1_2:64866692-64866711 None:intergenic 30.0%
CATAAGAAATGAATGAGTTG+TGG + chr1_2:64866787-64866806 Msa0085020:intron 30.0%
AATGAATGAGTTGTGGTATA+AGG + chr1_2:64866794-64866813 Msa0085020:intron 30.0%
!!! TATGTTATTTCCCACACTTT+TGG + chr1_2:64867044-64867063 Msa0085020:intron 30.0%
TAATTTCTGACATGAATTGC+AGG + chr1_2:64867133-64867152 Msa0085020:intron 30.0%
AATTTCTGACATGAATTGCA+GGG + chr1_2:64867134-64867153 Msa0085020:intron 30.0%
TGATTGATTATCTAGCATGC+GGG - chr1_2:64866045-64866064 None:intergenic 35.0%
TTTGGAAGTAAAGTCGAACA+AGG - chr1_2:64866118-64866137 None:intergenic 35.0%
ACTAATGCAGACACATAGAT+AGG - chr1_2:64866186-64866205 None:intergenic 35.0%
TTTGTTGCATCTCTTCAAGA+AGG - chr1_2:64866256-64866275 None:intergenic 35.0%
! CTTTGCATGACAGTTTTTAC+AGG - chr1_2:64866362-64866381 None:intergenic 35.0%
TTGATAGAGACGTTAGATGT+AGG + chr1_2:64866460-64866479 Msa0085020:intron 35.0%
GATTAGATGTCTATTCCCAA+TGG + chr1_2:64866754-64866773 Msa0085020:intron 35.0%
TTATGACCAGTTTGTCCATT+GGG - chr1_2:64866772-64866791 None:intergenic 35.0%
ACATATGTCATGTCACTGAA+AGG + chr1_2:64866824-64866843 Msa0085020:intron 35.0%
CATACAATACACCAATTGGT+GGG + chr1_2:64866882-64866901 Msa0085020:intron 35.0%
AAATCATTAGCTGTCCAATC+TGG - chr1_2:64867072-64867091 None:intergenic 35.0%
ATTTCTGACATGAATTGCAG+GGG + chr1_2:64867135-64867154 Msa0085020:intron 35.0%
GTTTCGAATTCAGATAACGA+AGG + chr1_2:64867245-64867264 Msa0085020:CDS 35.0%
CTATTGCAAACTGCTTAACA+CGG + chr1_2:64867279-64867298 Msa0085020:CDS 35.0%
! AAGAAAAGAGTGTACAGTAG+GGG - chr1_2:64867321-64867340 None:intergenic 35.0%
! CAAGAAAAGAGTGTACAGTA+GGG - chr1_2:64867322-64867341 None:intergenic 35.0%
AAGAACAATGGCAAAGCAAA+TGG - chr1_2:64867683-64867702 None:intergenic 35.0%
! TTGCTTTGCCATTGTTCTTT+TGG + chr1_2:64867684-64867703 Msa0085020:three_prime_UTR 35.0%
ATCGTGAACCAAAAGAACAA+TGG - chr1_2:64867695-64867714 None:intergenic 35.0%
TGATTGATTATCTAGCATGC+GGG - chr1_2:64866045-64866064 None:intergenic 35.0%
TTTGGAAGTAAAGTCGAACA+AGG - chr1_2:64866118-64866137 None:intergenic 35.0%
ACTAATGCAGACACATAGAT+AGG - chr1_2:64866186-64866205 None:intergenic 35.0%
TTTGTTGCATCTCTTCAAGA+AGG - chr1_2:64866256-64866275 None:intergenic 35.0%
! CTTTGCATGACAGTTTTTAC+AGG - chr1_2:64866362-64866381 None:intergenic 35.0%
TTGATAGAGACGTTAGATGT+AGG + chr1_2:64866460-64866479 Msa0085020:intron 35.0%
GATTAGATGTCTATTCCCAA+TGG + chr1_2:64866754-64866773 Msa0085020:intron 35.0%
TTATGACCAGTTTGTCCATT+GGG - chr1_2:64866772-64866791 None:intergenic 35.0%
ACATATGTCATGTCACTGAA+AGG + chr1_2:64866824-64866843 Msa0085020:intron 35.0%
CATACAATACACCAATTGGT+GGG + chr1_2:64866882-64866901 Msa0085020:intron 35.0%
AAATCATTAGCTGTCCAATC+TGG - chr1_2:64867072-64867091 None:intergenic 35.0%
ATTTCTGACATGAATTGCAG+GGG + chr1_2:64867135-64867154 Msa0085020:intron 35.0%
GTTTCGAATTCAGATAACGA+AGG + chr1_2:64867245-64867264 Msa0085020:CDS 35.0%
CTATTGCAAACTGCTTAACA+CGG + chr1_2:64867279-64867298 Msa0085020:CDS 35.0%
! AAGAAAAGAGTGTACAGTAG+GGG - chr1_2:64867321-64867340 None:intergenic 35.0%
! CAAGAAAAGAGTGTACAGTA+GGG - chr1_2:64867322-64867341 None:intergenic 35.0%
AAGAACAATGGCAAAGCAAA+TGG - chr1_2:64867683-64867702 None:intergenic 35.0%
! TTGCTTTGCCATTGTTCTTT+TGG + chr1_2:64867684-64867703 Msa0085020:three_prime_UTR 35.0%
ATCGTGAACCAAAAGAACAA+TGG - chr1_2:64867695-64867714 None:intergenic 35.0%
AAAACACCACACAACAACAG+TGG + chr1_2:64865472-64865491 Msa0085020:CDS 40.0%
TTACGAGGCAAAAAGCAACT+TGG - chr1_2:64865498-64865517 None:intergenic 40.0%
!! CAAATCTTGCAATGGGTTAG+TGG + chr1_2:64865770-64865789 Msa0085020:intron 40.0%
! CTGCTTGTGTTTAGGACTTT+AGG + chr1_2:64866000-64866019 Msa0085020:intron 40.0%
! TAGTGTTTGAAACATGTGGC+TGG + chr1_2:64866604-64866623 Msa0085020:intron 40.0%
TCTATTCCCAATGGACAAAC+TGG + chr1_2:64866763-64866782 Msa0085020:intron 40.0%
CTTATGACCAGTTTGTCCAT+TGG - chr1_2:64866773-64866792 None:intergenic 40.0%
AGGTTCAAGTCCTAGAAACA+GGG + chr1_2:64866851-64866870 Msa0085020:intron 40.0%
GCTGCATACAATACACCAAT+TGG + chr1_2:64866878-64866897 Msa0085020:intron 40.0%
GCATACAATACACCAATTGG+TGG + chr1_2:64866881-64866900 Msa0085020:intron 40.0%
TTCAACCACCTTGCTTAGAA+GGG - chr1_2:64866983-64867002 None:intergenic 40.0%
ATTCAACCACCTTGCTTAGA+AGG - chr1_2:64866984-64867003 None:intergenic 40.0%
! GTGACAGATTTTCCACACTA+TGG - chr1_2:64867202-64867221 None:intergenic 40.0%
! AGAAAAGAGTGTACAGTAGG+GGG - chr1_2:64867320-64867339 None:intergenic 40.0%
! GCAAGAAAAGAGTGTACAGT+AGG - chr1_2:64867323-64867342 None:intergenic 40.0%
! TCTCAGTGTTACTAGGTTGA+GGG - chr1_2:64867367-64867386 None:intergenic 40.0%
! ATCTCAGTGTTACTAGGTTG+AGG - chr1_2:64867368-64867387 None:intergenic 40.0%
!!! CACGATAGATTTTTTCGTCG+AGG + chr1_2:64867709-64867728 Msa0085020:three_prime_UTR 40.0%
!!! GATAGATTTTTTCGTCGAGG+AGG + chr1_2:64867712-64867731 Msa0085020:three_prime_UTR 40.0%
AAAACACCACACAACAACAG+TGG + chr1_2:64865472-64865491 Msa0085020:CDS 40.0%
TTACGAGGCAAAAAGCAACT+TGG - chr1_2:64865498-64865517 None:intergenic 40.0%
!! CAAATCTTGCAATGGGTTAG+TGG + chr1_2:64865770-64865789 Msa0085020:intron 40.0%
! CTGCTTGTGTTTAGGACTTT+AGG + chr1_2:64866000-64866019 Msa0085020:intron 40.0%
! TAGTGTTTGAAACATGTGGC+TGG + chr1_2:64866604-64866623 Msa0085020:intron 40.0%
TCTATTCCCAATGGACAAAC+TGG + chr1_2:64866763-64866782 Msa0085020:intron 40.0%
CTTATGACCAGTTTGTCCAT+TGG - chr1_2:64866773-64866792 None:intergenic 40.0%
AGGTTCAAGTCCTAGAAACA+GGG + chr1_2:64866851-64866870 Msa0085020:intron 40.0%
GCTGCATACAATACACCAAT+TGG + chr1_2:64866878-64866897 Msa0085020:intron 40.0%
GCATACAATACACCAATTGG+TGG + chr1_2:64866881-64866900 Msa0085020:intron 40.0%
TTCAACCACCTTGCTTAGAA+GGG - chr1_2:64866983-64867002 None:intergenic 40.0%
ATTCAACCACCTTGCTTAGA+AGG - chr1_2:64866984-64867003 None:intergenic 40.0%
! GTGACAGATTTTCCACACTA+TGG - chr1_2:64867202-64867221 None:intergenic 40.0%
! AGAAAAGAGTGTACAGTAGG+GGG - chr1_2:64867320-64867339 None:intergenic 40.0%
! GCAAGAAAAGAGTGTACAGT+AGG - chr1_2:64867323-64867342 None:intergenic 40.0%
! TCTCAGTGTTACTAGGTTGA+GGG - chr1_2:64867367-64867386 None:intergenic 40.0%
! ATCTCAGTGTTACTAGGTTG+AGG - chr1_2:64867368-64867387 None:intergenic 40.0%
!!! CACGATAGATTTTTTCGTCG+AGG + chr1_2:64867709-64867728 Msa0085020:three_prime_UTR 40.0%
!!! GATAGATTTTTTCGTCGAGG+AGG + chr1_2:64867712-64867731 Msa0085020:three_prime_UTR 40.0%
! AGCAGAGAAAGTGTGTGAGT+GGG - chr1_2:64865544-64865563 None:intergenic 45.0%
CTCTTCTCTTCTCTCAAACG+AGG + chr1_2:64865562-64865581 Msa0085020:CDS 45.0%
GCATTTCCGATACACCAGTA+TGG + chr1_2:64866683-64866702 Msa0085020:CDS 45.0%
TCATGTCACTGAAAGGTCAC+AGG + chr1_2:64866831-64866850 Msa0085020:intron 45.0%
CAGGTTCAAGTCCTAGAAAC+AGG + chr1_2:64866850-64866869 Msa0085020:intron 45.0%
CAAGTCCTAGAAACAGGGTA+AGG + chr1_2:64866856-64866875 Msa0085020:intron 45.0%
TTAAAGCTCCCGTATACGCA+GGG - chr1_2:64866921-64866940 None:intergenic 45.0%
ATTAAAGCTCCCGTATACGC+AGG - chr1_2:64866922-64866941 None:intergenic 45.0%
! ATACGGGAGCTTTAATGCAC+CGG + chr1_2:64866926-64866945 Msa0085020:intron 45.0%
GCTAAAAAAAAAGGGCAGCC+CGG - chr1_2:64866948-64866967 None:intergenic 45.0%
CTTGCTTAGAAGGGTCAACT+TGG - chr1_2:64866974-64866993 None:intergenic 45.0%
! CAAGTTGACCCTTCTAAGCA+AGG + chr1_2:64866972-64866991 Msa0085020:intron 45.0%
! CAATCTGGCACCAAAAGTGT+GGG - chr1_2:64867057-64867076 None:intergenic 45.0%
ATGTCTTGTGTGCCATAGTG+TGG + chr1_2:64867187-64867206 Msa0085020:CDS 45.0%
TGTCACCATCACACTCTTTC+AGG + chr1_2:64867216-64867235 Msa0085020:CDS 45.0%
! GATCTCCTGAAAGAGTGTGA+TGG - chr1_2:64867224-64867243 None:intergenic 45.0%
! ACTAGGTTGAGGGGTAACTT+TGG - chr1_2:64867357-64867376 None:intergenic 45.0%
! CTCAGTGTTACTAGGTTGAG+GGG - chr1_2:64867366-64867385 None:intergenic 45.0%
! CCAGGTATCTCAGTGTTACT+AGG - chr1_2:64867374-64867393 None:intergenic 45.0%
CCTAGTAACACTGAGATACC+TGG + chr1_2:64867371-64867390 Msa0085020:CDS 45.0%
TTGTACTATGTCCCTCCTCA+CGG - chr1_2:64867423-64867442 None:intergenic 45.0%
GGAGGGACATAGTACAAGAT+TGG + chr1_2:64867426-64867445 Msa0085020:CDS 45.0%
AACGTGCTGGAGAAAGACTA+AGG + chr1_2:64867481-64867500 Msa0085020:three_prime_UTR 45.0%
ACGTGCTGGAGAAAGACTAA+GGG + chr1_2:64867482-64867501 Msa0085020:three_prime_UTR 45.0%
! TGCGAAGCTTGCTTAAGTTG+GGG - chr1_2:64865451-64865470 None:intergenic 45.0%
! CTGCGAAGCTTGCTTAAGTT+GGG - chr1_2:64865452-64865471 None:intergenic 45.0%
! TCTGCGAAGCTTGCTTAAGT+TGG - chr1_2:64865453-64865472 None:intergenic 45.0%
! AGCAGAGAAAGTGTGTGAGT+GGG - chr1_2:64865544-64865563 None:intergenic 45.0%
CTCTTCTCTTCTCTCAAACG+AGG + chr1_2:64865562-64865581 Msa0085020:CDS 45.0%
GCATTTCCGATACACCAGTA+TGG + chr1_2:64866683-64866702 Msa0085020:CDS 45.0%
TCATGTCACTGAAAGGTCAC+AGG + chr1_2:64866831-64866850 Msa0085020:intron 45.0%
CAGGTTCAAGTCCTAGAAAC+AGG + chr1_2:64866850-64866869 Msa0085020:intron 45.0%
CAAGTCCTAGAAACAGGGTA+AGG + chr1_2:64866856-64866875 Msa0085020:intron 45.0%
TTAAAGCTCCCGTATACGCA+GGG - chr1_2:64866921-64866940 None:intergenic 45.0%
ATTAAAGCTCCCGTATACGC+AGG - chr1_2:64866922-64866941 None:intergenic 45.0%
! ATACGGGAGCTTTAATGCAC+CGG + chr1_2:64866926-64866945 Msa0085020:intron 45.0%
GCTAAAAAAAAAGGGCAGCC+CGG - chr1_2:64866948-64866967 None:intergenic 45.0%
CTTGCTTAGAAGGGTCAACT+TGG - chr1_2:64866974-64866993 None:intergenic 45.0%
! CAAGTTGACCCTTCTAAGCA+AGG + chr1_2:64866972-64866991 Msa0085020:intron 45.0%
! CAATCTGGCACCAAAAGTGT+GGG - chr1_2:64867057-64867076 None:intergenic 45.0%
ATGTCTTGTGTGCCATAGTG+TGG + chr1_2:64867187-64867206 Msa0085020:CDS 45.0%
TGTCACCATCACACTCTTTC+AGG + chr1_2:64867216-64867235 Msa0085020:CDS 45.0%
! GATCTCCTGAAAGAGTGTGA+TGG - chr1_2:64867224-64867243 None:intergenic 45.0%
! ACTAGGTTGAGGGGTAACTT+TGG - chr1_2:64867357-64867376 None:intergenic 45.0%
! CTCAGTGTTACTAGGTTGAG+GGG - chr1_2:64867366-64867385 None:intergenic 45.0%
! CCAGGTATCTCAGTGTTACT+AGG - chr1_2:64867374-64867393 None:intergenic 45.0%
CCTAGTAACACTGAGATACC+TGG + chr1_2:64867371-64867390 Msa0085020:CDS 45.0%
TTGTACTATGTCCCTCCTCA+CGG - chr1_2:64867423-64867442 None:intergenic 45.0%
GGAGGGACATAGTACAAGAT+TGG + chr1_2:64867426-64867445 Msa0085020:CDS 45.0%
AACGTGCTGGAGAAAGACTA+AGG + chr1_2:64867481-64867500 Msa0085020:three_prime_UTR 45.0%
ACGTGCTGGAGAAAGACTAA+GGG + chr1_2:64867482-64867501 Msa0085020:three_prime_UTR 45.0%
ACTTGGCCACTGTTGTTGTG+TGG - chr1_2:64865481-64865500 None:intergenic 50.0%
! GAGCAGAGAAAGTGTGTGAG+TGG - chr1_2:64865545-64865564 None:intergenic 50.0%
! TGCAGCCTTACCCTGTTTCT+AGG - chr1_2:64866864-64866883 None:intergenic 50.0%
! TACGGGAGCTTTAATGCACC+GGG + chr1_2:64866927-64866946 Msa0085020:intron 50.0%
GTTGACCCTTCTAAGCAAGG+TGG + chr1_2:64866975-64866994 Msa0085020:intron 50.0%
CCAATCTGGCACCAAAAGTG+TGG - chr1_2:64867058-64867077 None:intergenic 50.0%
!!! CCACACTTTTGGTGCCAGAT+TGG + chr1_2:64867055-64867074 Msa0085020:intron 50.0%
TTGTAAGAAGTCGTGCCGTG+AGG + chr1_2:64867405-64867424 Msa0085020:CDS 50.0%
! CCAGCACGTTGTTGACTAAG+CGG - chr1_2:64867471-64867490 None:intergenic 50.0%
ACTTGGCCACTGTTGTTGTG+TGG - chr1_2:64865481-64865500 None:intergenic 50.0%
! GAGCAGAGAAAGTGTGTGAG+TGG - chr1_2:64865545-64865564 None:intergenic 50.0%
! TGCAGCCTTACCCTGTTTCT+AGG - chr1_2:64866864-64866883 None:intergenic 50.0%
! TACGGGAGCTTTAATGCACC+GGG + chr1_2:64866927-64866946 Msa0085020:intron 50.0%
GTTGACCCTTCTAAGCAAGG+TGG + chr1_2:64866975-64866994 Msa0085020:intron 50.0%
CCAATCTGGCACCAAAAGTG+TGG - chr1_2:64867058-64867077 None:intergenic 50.0%
!!! CCACACTTTTGGTGCCAGAT+TGG + chr1_2:64867055-64867074 Msa0085020:intron 50.0%
TTGTAAGAAGTCGTGCCGTG+AGG + chr1_2:64867405-64867424 Msa0085020:CDS 50.0%
! CCAGCACGTTGTTGACTAAG+CGG - chr1_2:64867471-64867490 None:intergenic 50.0%
TTGCAGGGGAAGACTGTGAG+AGG + chr1_2:64867149-64867168 Msa0085020:intron 55.0%
GCACGACTTCTTACAAGCCG+CGG - chr1_2:64867401-64867420 None:intergenic 55.0%
TAAGAAGTCGTGCCGTGAGG+AGG + chr1_2:64867408-64867427 Msa0085020:CDS 55.0%
AAGAAGTCGTGCCGTGAGGA+GGG + chr1_2:64867409-64867428 Msa0085020:CDS 55.0%
CCGCTTAGTCAACAACGTGC+TGG + chr1_2:64867468-64867487 Msa0085020:exon 55.0%
TTGCAGGGGAAGACTGTGAG+AGG + chr1_2:64867149-64867168 Msa0085020:intron 55.0%
GCACGACTTCTTACAAGCCG+CGG - chr1_2:64867401-64867420 None:intergenic 55.0%
TAAGAAGTCGTGCCGTGAGG+AGG + chr1_2:64867408-64867427 Msa0085020:CDS 55.0%
AAGAAGTCGTGCCGTGAGGA+GGG + chr1_2:64867409-64867428 Msa0085020:CDS 55.0%
CCGCTTAGTCAACAACGTGC+TGG + chr1_2:64867468-64867487 Msa0085020:exon 55.0%
CAATTGGTGGGACCCCTTCC+CGG + chr1_2:64866894-64866913 Msa0085020:intron 60.0%
TATACGCAGGGTCCGGGAAG+GGG - chr1_2:64866909-64866928 None:intergenic 60.0%
GTATACGCAGGGTCCGGGAA+GGG - chr1_2:64866910-64866929 None:intergenic 60.0%
CTTCCCGGACCCTGCGTATA+CGG + chr1_2:64866909-64866928 Msa0085020:intron 60.0%
TTCCCGGACCCTGCGTATAC+GGG + chr1_2:64866910-64866929 Msa0085020:intron 60.0%
CTGAGATACCTGGTCCTCCG+CGG + chr1_2:64867381-64867400 Msa0085020:CDS 60.0%
CGACTTCTTACAAGCCGCGG+AGG - chr1_2:64867398-64867417 None:intergenic 60.0%
CAATTGGTGGGACCCCTTCC+CGG + chr1_2:64866894-64866913 Msa0085020:intron 60.0%
TATACGCAGGGTCCGGGAAG+GGG - chr1_2:64866909-64866928 None:intergenic 60.0%
GTATACGCAGGGTCCGGGAA+GGG - chr1_2:64866910-64866929 None:intergenic 60.0%
CTTCCCGGACCCTGCGTATA+CGG + chr1_2:64866909-64866928 Msa0085020:intron 60.0%
TTCCCGGACCCTGCGTATAC+GGG + chr1_2:64866910-64866929 Msa0085020:intron 60.0%
CTGAGATACCTGGTCCTCCG+CGG + chr1_2:64867381-64867400 Msa0085020:CDS 60.0%
CGACTTCTTACAAGCCGCGG+AGG - chr1_2:64867398-64867417 None:intergenic 60.0%
CGGGAAGGGGTCCCACCAAT+TGG - chr1_2:64866896-64866915 None:intergenic 65.0%
CGTATACGCAGGGTCCGGGA+AGG - chr1_2:64866911-64866930 None:intergenic 65.0%
CTCCCGTATACGCAGGGTCC+GGG - chr1_2:64866915-64866934 None:intergenic 65.0%
GCTCCCGTATACGCAGGGTC+CGG - chr1_2:64866916-64866935 None:intergenic 65.0%
CTTACAAGCCGCGGAGGACC+AGG - chr1_2:64867392-64867411 None:intergenic 65.0%
CGGGAAGGGGTCCCACCAAT+TGG - chr1_2:64866896-64866915 None:intergenic 65.0%
CGTATACGCAGGGTCCGGGA+AGG - chr1_2:64866911-64866930 None:intergenic 65.0%
CTCCCGTATACGCAGGGTCC+GGG - chr1_2:64866915-64866934 None:intergenic 65.0%
GCTCCCGTATACGCAGGGTC+CGG - chr1_2:64866916-64866935 None:intergenic 65.0%
CTTACAAGCCGCGGAGGACC+AGG - chr1_2:64867392-64867411 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 64865451 64867780 64865451 ID=Msa0085020;Name=Msa0085020
chr1_2 mRNA 64865451 64867780 64865451 ID=Msa0085020-mRNA-1;Parent=Msa0085020;Name=Msa0085020-mRNA-1;_AED=0.29;_eAED=0.32;_QI=0|0|0.25|1|0.33|0.25|4|305|214
chr1_2 exon 64865451 64865671 64865451 ID=Msa0085020-mRNA-1:exon:21911;Parent=Msa0085020-mRNA-1
chr1_2 exon 64865948 64865966 64865948 ID=Msa0085020-mRNA-1:exon:21912;Parent=Msa0085020-mRNA-1
chr1_2 exon 64866644 64866727 64866644 ID=Msa0085020-mRNA-1:exon:21913;Parent=Msa0085020-mRNA-1
chr1_2 exon 64867155 64867780 64867155 ID=Msa0085020-mRNA-1:exon:21914;Parent=Msa0085020-mRNA-1
chr1_2 CDS 64865451 64865671 64865451 ID=Msa0085020-mRNA-1:cds;Parent=Msa0085020-mRNA-1
chr1_2 CDS 64865948 64865966 64865948 ID=Msa0085020-mRNA-1:cds;Parent=Msa0085020-mRNA-1
chr1_2 CDS 64866644 64866727 64866644 ID=Msa0085020-mRNA-1:cds;Parent=Msa0085020-mRNA-1
chr1_2 CDS 64867155 64867475 64867155 ID=Msa0085020-mRNA-1:cds;Parent=Msa0085020-mRNA-1
chr1_2 three_prime_UTR 64867476 64867780 64867476 ID=Msa0085020-mRNA-1:three_prime_utr;Parent=Msa0085020-mRNA-1
Gene Sequence

>Msa0085020

CAACTTAAGCAAGCTTCGCAGAAAACACCACACAACAACAGTGGCCAAGTTGCTTTTTGCCTCGTAAAAGATCTTATCAAATCTTCTTGTCCCACTCACACACTTTCTCTGCTCTTCTCTTCTCTCAAACGAGGTTTGTGTCCCTCTCATTCCGTTTTTTTTTTCTTTCTGCTTCATCTTGATTCAACTGAAACCCTTCTTTTCAATTTCACTGATTTTGGCTTTGAACTAGTTGAAGTTATAGGTAGAGATTATAAGGTTCAAAATTTTGGTAATGTGGCATTTCCGATACACCAGTATGGTTATAAAGTTACAATGTTTGAGGGGAAGACTGTGAGAGGCACAATGAGTCTAGCATGTCTTGTGTGCCATAGTGTGGAAAATCTGTCACCATCACACTCTTTCAGGAGATCTGTTTCGAATTCAGATAACGAAGGAAGATGTTATGCTATTGCAAACTGCTTAACACGGAAGTTATCTGTTCAACCCCCTACTGTACACTCTTTTCTTGCAGCATCAACTTCCAAAGTTACCCCTCAACCTAGTAACACTGAGATACCTGGTCCTCCGCGGCTTGTAAGAAGTCGTGCCGTGAGGAGGGACATAGTACAAGATTGGAATTTTGATGAAATTGTAGCCGCTTAG
Protein sequence

>Msa0085020

QLKQASQKTPHNNSGQVAFCLVKDLIKSSCPTHTLSLLFSSLKRGLCPSHSVFFFFLLHLDSTETLLFNFTDFGFELVEVIGRDYKVQNFGNVAFPIHQYGYKVTMFEGKTVRGTMSLACLVCHSVENLSPSHSFRRSVSNSDNEGRCYAIANCLTRKLSVQPPTVHSFLAASTSKVTPQPSNTEIPGPPRLVRSRAVRRDIVQDWNFDEIVAA*