AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0085670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085670 MtrunA17_Chr6g0467121 48.056 360 156 8 10 356 43 384 7.18e-110 326
Msa0085670 MtrunA17_Chr6g0467161 47.041 338 150 8 10 338 19 336 1.51e-96 290
Msa0085670 MtrunA17_Chr6g0467251 41.279 344 182 6 4 342 5 333 1.33e-82 255
Msa0085670 MtrunA17_Chr2g0292151 44.245 278 142 7 87 354 73 347 1.20e-68 219
Msa0085670 MtrunA17_Chr2g0292271 43.321 277 145 6 87 354 73 346 4.06e-68 218
Msa0085670 MtrunA17_Chr2g0292091 43.939 264 136 6 87 341 68 328 1.63e-67 216
Msa0085670 MtrunA17_Chr2g0292211 44.697 264 134 6 87 341 84 344 2.90e-67 216
Msa0085670 MtrunA17_Chr5g0404721 45.098 255 131 5 87 334 74 326 7.86e-67 214
Msa0085670 MtrunA17_Chr4g0013171 37.908 306 168 7 38 338 33 321 8.66e-67 213
Msa0085670 MtrunA17_Chr2g0291751 44.106 263 137 5 87 341 73 333 1.45e-66 214
Msa0085670 MtrunA17_Chr3g0128061 46.743 261 126 6 87 338 70 326 2.46e-66 212
Msa0085670 MtrunA17_Chr8g0372601 38.854 314 168 8 34 338 32 330 2.72e-66 213
Msa0085670 MtrunA17_Chr1g0188431 41.935 248 137 4 87 334 83 323 8.85e-66 211
Msa0085670 MtrunA17_Chr2g0292251 41.818 275 148 6 89 354 74 345 9.82e-66 212
Msa0085670 MtrunA17_Chr1g0204011 43.629 259 132 6 87 338 71 322 1.57e-65 210
Msa0085670 MtrunA17_Chr2g0292241 44.318 264 135 6 87 341 73 333 2.72e-65 211
Msa0085670 MtrunA17_Chr2g0322861 45.594 261 128 7 87 338 131 386 4.13e-65 211
Msa0085670 MtrunA17_Chr7g0225221 44.314 255 136 5 87 338 69 320 4.59e-65 209
Msa0085670 MtrunA17_Chr8g0371381 45.174 259 126 7 87 338 70 319 9.07e-65 208
Msa0085670 MtrunA17_Chr2g0292101 42.909 275 147 5 87 353 69 341 2.35e-64 208
Msa0085670 MtrunA17_Chr6g0477071 42.339 248 136 4 87 334 98 338 4.16e-64 207
Msa0085670 MtrunA17_Chr5g0405991 41.961 255 132 7 87 334 67 312 1.76e-63 204
Msa0085670 MtrunA17_Chr5g0432441 42.802 257 138 4 87 338 74 326 5.64e-63 204
Msa0085670 MtrunA17_Chr1g0194151 45.247 263 130 7 87 338 74 333 7.64e-63 203
Msa0085670 MtrunA17_Chr2g0292111 41.091 275 150 6 87 352 73 344 9.24e-63 204
Msa0085670 MtrunA17_Chr4g0053871 41.502 253 144 3 87 336 71 322 1.60e-62 202
Msa0085670 MtrunA17_Chr4g0038161 35.569 343 193 7 1 338 1 320 1.82e-62 202
Msa0085670 MtrunA17_Chr7g0268501 37.798 336 179 10 13 338 5 320 1.47e-61 200
Msa0085670 MtrunA17_Chr7g0268521 37.798 336 179 10 13 338 5 320 1.47e-61 200
Msa0085670 MtrunA17_Chr2g0320071 41.107 253 144 4 87 338 69 317 1.61e-61 199
Msa0085670 MtrunA17_Chr2g0320101 41.502 253 143 4 87 338 69 317 1.85e-61 199
Msa0085670 MtrunA17_Chr2g0292181 41.603 262 141 6 89 341 84 342 1.92e-61 201
Msa0085670 MtrunA17_Chr3g0106921 42.169 249 138 3 89 334 71 316 2.63e-61 199
Msa0085670 MtrunA17_Chr7g0258811 42.412 257 137 6 87 334 113 367 2.69e-61 201
Msa0085670 MtrunA17_Chr4g0038151 37.333 300 167 6 43 338 39 321 3.31e-61 199
Msa0085670 MtrunA17_Chr4g0043391 35.608 337 188 9 10 338 9 324 5.36e-61 198
Msa0085670 MtrunA17_Chr4g0024121 41.699 259 141 5 87 338 71 326 6.18e-61 198
Msa0085670 MtrunA17_Chr4g0038171 35.821 335 186 9 12 338 8 321 1.21e-60 197
Msa0085670 MtrunA17_Chr1g0155611 42.188 256 138 5 87 334 90 343 1.31e-60 198
Msa0085670 MtrunA17_Chr5g0400331 41.132 265 136 7 89 338 72 331 2.50e-60 197
Msa0085670 MtrunA17_Chr1g0194271 43.007 286 130 8 83 338 58 340 5.73e-60 196
Msa0085670 MtrunA17_Chr4g0013181 36.275 306 173 7 38 338 65 353 7.88e-60 196
Msa0085670 MtrunA17_Chr3g0114451 34.835 333 186 9 11 334 4 314 1.55e-59 194
Msa0085670 MtrunA17_Chr2g0292261 41.877 277 148 7 87 354 73 345 1.64e-59 196
Msa0085670 MtrunA17_Chr7g0244461 41.339 254 142 5 87 338 64 312 3.06e-59 194
Msa0085670 MtrunA17_Chr2g0299691 42.586 263 136 7 87 338 100 358 3.99e-59 195
Msa0085670 MtrunA17_Chr1g0164891 42.366 262 139 7 87 338 133 392 7.46e-59 195
Msa0085670 MtrunA17_Chr4g0046131 42.146 261 140 5 87 337 86 345 1.16e-58 193
Msa0085670 MtrunA17_Chr1g0175561 40.698 258 140 7 87 334 68 322 1.35e-58 192
Msa0085670 MtrunA17_Chr4g0070951 42.366 262 137 6 87 338 72 329 1.37e-58 192
Msa0085670 MtrunA17_Chr7g0254201 40.625 256 143 4 87 339 64 313 2.15e-58 191
Msa0085670 MtrunA17_Chr7g0244451 42.800 250 136 5 87 334 68 312 3.01e-58 191
Msa0085670 MtrunA17_Chr7g0254231 39.286 252 147 3 87 338 66 311 3.88e-58 191
Msa0085670 MtrunA17_Chr5g0402581 41.923 260 139 5 87 338 71 326 4.05e-58 191
Msa0085670 MtrunA17_Chr1g0157051 40.467 257 138 7 87 336 68 316 4.25e-58 191
Msa0085670 MtrunA17_Chr5g0402571 40.927 259 143 5 87 338 71 326 4.56e-58 191
Msa0085670 MtrunA17_Chr5g0401431 36.036 333 190 9 14 336 8 327 4.56e-58 191
Msa0085670 MtrunA17_Chr4g0072091 41.538 260 140 6 87 338 68 323 8.41e-58 190
Msa0085670 MtrunA17_Chr5g0432351 40.927 259 144 6 87 340 69 323 2.16e-57 189
Msa0085670 MtrunA17_Chr5g0438021 39.841 251 144 4 87 334 75 321 2.51e-57 189
Msa0085670 MtrunA17_Chr2g0279041 41.985 262 135 6 87 338 71 325 4.28e-57 188
Msa0085670 MtrunA17_Chr3g0129051 40.239 251 143 4 87 334 72 318 4.63e-57 188
Msa0085670 MtrunA17_Chr4g0013821 38.033 305 164 7 40 338 39 324 8.59e-57 188
Msa0085670 MtrunA17_Chr4g0013721 42.578 256 136 5 89 338 146 396 9.65e-57 190
Msa0085670 MtrunA17_Chr2g0292291 39.350 277 155 6 87 354 74 346 1.08e-56 188
Msa0085670 MtrunA17_Chr2g0320091 39.764 254 147 4 87 338 69 318 1.14e-56 187
Msa0085670 MtrunA17_Chr6g0460711 39.608 255 146 3 87 338 65 314 1.35e-56 187
Msa0085670 MtrunA17_Chr1g0148021 34.302 344 190 10 10 338 6 328 1.50e-56 187
Msa0085670 MtrunA17_Chr2g0320061 40.945 254 144 5 87 338 69 318 2.13e-56 186
Msa0085670 MtrunA17_Chr5g0421411 37.500 264 150 7 87 338 25 285 5.91e-56 184
Msa0085670 MtrunA17_Chr1g0197411 38.996 259 149 4 89 339 74 331 1.69e-55 184
Msa0085670 MtrunA17_Chr2g0279061 41.221 262 137 6 87 338 12 266 3.87e-55 181
Msa0085670 MtrunA17_Chr2g0320051 40.157 254 146 5 87 338 69 318 5.18e-55 183
Msa0085670 MtrunA17_Chr2g0320031 38.976 254 149 5 87 338 70 319 5.44e-55 183
Msa0085670 MtrunA17_Chr5g0432341 40.310 258 145 6 87 339 69 322 6.81e-55 182
Msa0085670 MtrunA17_Chr7g0224371 39.921 253 146 4 87 338 75 322 3.34e-54 181
Msa0085670 MtrunA17_Chr4g0068621 34.311 341 193 10 14 338 11 336 4.94e-53 178
Msa0085670 MtrunA17_Chr4g0013201 40.551 254 144 5 87 338 90 338 7.39e-53 178
Msa0085670 MtrunA17_Chr8g0364291 37.984 258 150 4 89 338 69 324 8.77e-53 177
Msa0085670 MtrunA17_Chr4g0013191 41.406 256 141 6 87 338 90 340 1.15e-52 177
Msa0085670 MtrunA17_Chr2g0296621 38.996 259 142 7 86 338 93 341 1.68e-52 177
Msa0085670 MtrunA17_Chr1g0181511 34.098 305 182 6 49 340 27 325 6.92e-52 175
Msa0085670 MtrunA17_Chr3g0129061 39.442 251 145 4 87 334 72 318 1.09e-51 174
Msa0085670 MtrunA17_Chr4g0076211 37.452 259 147 6 87 339 85 334 2.76e-51 174
Msa0085670 MtrunA17_Chr5g0432331 38.224 259 149 7 87 339 100 353 3.17e-51 174
Msa0085670 MtrunA17_Chr4g0075241 37.984 258 151 5 87 338 73 327 4.73e-51 173
Msa0085670 MtrunA17_Chr4g0063671 39.773 264 139 8 87 338 65 320 1.31e-50 171
Msa0085670 MtrunA17_Chr2g0292281 42.982 228 111 6 134 354 15 230 4.73e-50 167
Msa0085670 MtrunA17_Chr1g0181431 34.496 258 162 3 87 340 71 325 2.54e-49 168
Msa0085670 MtrunA17_Chr6g0451571 38.247 251 149 3 89 334 81 330 3.25e-49 168
Msa0085670 MtrunA17_Chr3g0129041 39.442 251 145 4 87 334 72 318 4.39e-49 167
Msa0085670 MtrunA17_Chr3g0106901 33.233 331 190 9 10 334 14 319 7.66e-49 167
Msa0085670 MtrunA17_Chr3g0107881 38.372 258 149 8 87 338 71 324 1.04e-48 166
Msa0085670 MtrunA17_Chr4g0074851 33.977 259 164 3 86 340 75 330 1.74e-48 166
Msa0085670 MtrunA17_Chr1g0181481 34.749 259 162 3 86 340 70 325 3.55e-48 165
Msa0085670 MtrunA17_Chr2g0329641 38.168 262 144 6 89 339 82 336 6.30e-47 162
Msa0085670 MtrunA17_Chr1g0213061 32.565 347 199 12 1 338 1 321 5.98e-46 159
Msa0085670 MtrunA17_Chr3g0136751 36.538 260 151 5 87 338 69 322 3.21e-43 152
Msa0085670 MtrunA17_Chr2g0311251 32.886 298 156 8 76 335 46 337 8.70e-43 151
Msa0085670 MtrunA17_Chr2g0278671 34.351 262 156 7 87 338 68 323 2.10e-40 145
Msa0085670 MtrunA17_Chr2g0316481 32.753 287 152 7 87 335 120 403 1.01e-39 145
Msa0085670 MtrunA17_Chr2g0316791 32.404 287 153 7 87 335 120 403 1.47e-39 144
Msa0085670 MtrunA17_Chr2g0316831 32.056 287 154 7 87 335 156 439 8.40e-38 140
Msa0085670 MtrunA17_Chr2g0316191 31.944 288 154 7 87 335 120 404 1.03e-37 139
Msa0085670 MtrunA17_Chr5g0413051 33.462 260 162 5 89 338 119 377 3.53e-37 137
Msa0085670 MtrunA17_Chr3g0114461 36.559 186 113 4 155 338 10 192 5.77e-36 129
Msa0085670 MtrunA17_Chr2g0311241 28.659 328 151 8 87 335 104 427 2.68e-34 130
Msa0085670 MtrunA17_Chr5g0432401 32.677 254 121 8 87 334 73 282 5.72e-34 127
Msa0085670 MtrunA17_Chr4g0004591 32.051 234 129 5 129 335 123 353 3.47e-29 115
Msa0085670 MtrunA17_Chr2g0316141 29.371 286 142 8 89 335 11 275 1.16e-28 112
Msa0085670 MtrunA17_Chr2g0316181 29.371 286 142 8 89 335 11 275 1.16e-28 112
Msa0085670 MtrunA17_Chr1g0181501 32.461 191 121 4 155 340 4 191 6.52e-26 102
Msa0085670 MtrunA17_Chr5g0432321 37.815 119 72 1 165 283 16 132 2.42e-20 85.9
Msa0085670 MtrunA17_Chr2g0316811 39.683 126 71 3 87 210 94 216 2.01e-19 87.8
Msa0085670 MtrunA17_Chr3g0104101 32.394 142 83 5 209 338 9 149 8.17e-18 79.3
Msa0085670 MtrunA17_Chr1g0196191 44.706 85 45 1 67 151 9 91 5.29e-17 77.4
Msa0085670 MtrunA17_Chr7g0268491 51.351 74 35 1 265 338 25 97 5.46e-16 72.8
Msa0085670 MtrunA17_Chr5g0423911 51.515 66 32 0 269 334 2 67 3.04e-15 71.2
Msa0085670 MtrunA17_Chr7g0254181 38.318 107 60 3 105 211 22 122 3.87e-14 69.3
Msa0085670 MtrunA17_Chr4g0013811 45.946 74 39 1 154 227 5 77 1.53e-13 65.5
Msa0085670 MtrunA17_Chr6g0468731 47.761 67 30 2 87 153 27 88 7.63e-12 61.2
Msa0085670 MtrunA17_Chr3g0114491 46.154 65 34 1 87 151 62 125 1.04e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0085670 AT1G71695.1 61.538 273 102 2 87 359 88 357 8.78e-126 366
Msa0085670 AT4G08780.1 38.012 342 185 9 24 354 16 341 8.66e-68 217
Msa0085670 AT4G36430.1 41.961 255 139 5 87 334 74 326 7.85e-67 214
Msa0085670 AT5G14130.1 43.798 258 136 4 87 338 76 330 1.10e-66 214
Msa0085670 AT5G14130.2 43.798 258 136 4 87 338 117 371 2.99e-66 214
Msa0085670 AT2G18150.1 41.961 255 139 5 87 334 80 332 5.83e-66 212
Msa0085670 AT5G51890.1 45.882 255 123 7 87 333 70 317 7.18e-66 211
Msa0085670 AT4G08770.1 37.135 342 188 9 24 354 16 341 3.29e-65 210
Msa0085670 AT3G32980.1 38.824 340 177 10 24 350 23 344 8.23e-65 209
Msa0085670 AT2G18140.1 38.811 286 154 6 56 334 60 331 1.12e-64 209
Msa0085670 AT1G05260.1 45.000 260 131 7 87 338 70 325 1.80e-64 208
Msa0085670 AT3G21770.1 45.560 259 127 7 87 336 73 326 5.37e-64 207
Msa0085670 AT5G06730.1 42.481 266 141 6 87 343 77 339 3.19e-63 206
Msa0085670 AT4G33420.1 43.145 248 134 4 87 334 81 321 4.72e-63 204
Msa0085670 AT5G66390.1 41.961 255 139 5 87 334 77 329 6.64e-63 204
Msa0085670 AT3G49120.1 37.037 351 193 10 11 350 12 345 1.12e-62 204
Msa0085670 AT4G33420.2 43.145 248 134 4 87 334 128 368 1.83e-62 204
Msa0085670 AT4G16270.1 44.061 261 131 8 87 338 94 348 9.98e-62 201
Msa0085670 AT5G58400.1 34.835 333 184 8 11 334 13 321 1.52e-61 200
Msa0085670 AT1G44970.1 41.154 260 143 5 87 338 89 346 1.43e-60 198
Msa0085670 AT1G14550.1 39.583 288 167 5 53 338 39 321 4.94e-60 196
Msa0085670 AT4G11290.1 37.059 340 185 9 11 338 4 326 6.32e-60 196
Msa0085670 AT2G18980.1 40.769 260 141 6 87 338 69 323 1.46e-59 195
Msa0085670 AT3G50990.1 41.762 261 143 6 87 340 85 343 1.59e-59 196
Msa0085670 AT5G05340.1 40.784 255 144 4 87 338 74 324 1.62e-59 195
Msa0085670 AT2G38390.1 43.396 265 131 8 87 338 75 333 1.96e-59 196
Msa0085670 AT4G26010.2 40.316 253 141 5 87 338 94 337 2.25e-59 195
Msa0085670 AT4G26010.1 40.316 253 141 5 87 338 66 309 2.69e-59 194
Msa0085670 AT5G42180.1 40.945 254 137 6 87 334 67 313 3.27e-59 194
Msa0085670 AT2G35380.1 38.111 307 152 9 50 338 49 335 5.41e-59 194
Msa0085670 AT3G49110.1 36.716 335 183 10 28 350 29 346 6.04e-59 194
Msa0085670 AT4G30170.1 40.000 260 143 6 87 338 71 325 7.21e-59 193
Msa0085670 AT3G03670.1 40.000 260 143 5 87 338 67 321 8.52e-59 193
Msa0085670 AT2G38380.1 42.642 265 133 8 87 338 75 333 1.52e-58 193
Msa0085670 AT5G58390.1 40.400 250 141 5 89 334 67 312 3.86e-58 191
Msa0085670 AT2G22420.1 41.245 257 140 5 89 339 69 320 4.18e-58 191
Msa0085670 AT4G37530.1 39.615 260 148 4 87 338 71 329 4.18e-58 191
Msa0085670 AT1G49570.1 41.634 257 139 4 87 334 92 346 7.12e-58 191
Msa0085670 AT5G06720.1 39.453 256 145 5 87 334 76 329 7.15e-58 191
Msa0085670 AT1G05240.1 41.154 260 141 6 87 338 70 325 1.29e-57 190
Msa0085670 AT1G05250.1 41.154 260 141 6 87 338 70 325 1.29e-57 190
Msa0085670 AT5G17820.1 41.502 253 132 5 87 334 68 309 2.21e-57 189
Msa0085670 AT5G19890.1 40.458 262 138 7 87 338 74 327 3.00e-57 189
Msa0085670 AT1G34510.1 39.764 254 143 5 86 338 65 309 3.66e-57 188
Msa0085670 AT3G01190.1 41.473 258 139 5 87 338 70 321 3.84e-57 189
Msa0085670 AT5G17820.2 41.502 253 132 5 87 334 121 362 2.27e-56 188
Msa0085670 AT5G40150.1 38.889 252 151 2 89 338 78 328 3.39e-56 186
Msa0085670 AT5G15180.1 39.147 258 147 5 87 338 76 329 7.27e-56 186
Msa0085670 AT1G68850.1 32.659 346 201 10 10 338 5 335 7.71e-56 186
Msa0085670 AT1G14540.1 36.858 331 188 9 10 338 4 315 7.49e-55 182
Msa0085670 AT5G67400.1 39.080 261 148 6 87 338 71 329 2.18e-54 182
Msa0085670 AT4G17690.1 38.824 255 153 2 87 339 70 323 3.05e-54 181
Msa0085670 AT4G37520.1 37.692 260 153 4 87 338 71 329 3.10e-54 181
Msa0085670 AT2G35380.2 41.502 253 122 8 104 338 3 247 4.85e-54 178
Msa0085670 AT3G28200.1 39.286 252 149 3 89 338 67 316 1.39e-53 179
Msa0085670 AT3G49960.1 37.548 261 152 6 87 338 71 329 7.18e-53 178
Msa0085670 AT2G34060.1 39.231 260 147 5 87 336 86 344 9.30e-53 178
Msa0085670 AT5G64120.1 32.754 345 192 10 12 338 6 328 4.27e-52 176
Msa0085670 AT4G37520.2 36.923 260 152 5 87 338 71 326 1.87e-51 174
Msa0085670 AT5G24070.1 39.623 265 141 6 87 340 81 337 2.44e-51 174
Msa0085670 AT4G21960.1 34.363 259 163 3 86 340 74 329 1.06e-50 172
Msa0085670 AT5G39580.1 38.760 258 145 6 87 338 69 319 2.09e-50 171
Msa0085670 AT5G64110.1 39.695 262 139 7 87 338 78 330 9.89e-49 167
Msa0085670 AT5G47000.1 37.052 251 154 3 87 334 77 326 2.06e-48 166
Msa0085670 AT5G19880.1 37.838 259 148 6 87 334 69 325 4.12e-48 166
Msa0085670 AT2G24800.1 39.080 261 144 6 87 338 75 329 5.72e-48 165
Msa0085670 AT4G33870.1 33.448 290 149 5 87 335 112 398 6.63e-48 167
Msa0085670 AT5G22410.1 35.417 336 180 12 12 334 10 321 6.97e-48 165
Msa0085670 AT2G41480.1 38.258 264 143 8 87 338 73 328 1.75e-47 164
Msa0085670 AT2G39040.1 40.377 265 141 8 87 338 90 350 5.07e-47 163
Msa0085670 AT1G24110.1 35.547 256 158 4 89 338 68 322 1.18e-46 162
Msa0085670 AT2G43480.1 38.132 257 140 6 89 334 83 331 3.57e-46 160
Msa0085670 AT4G31760.1 36.154 260 152 5 87 338 73 326 3.65e-46 160
Msa0085670 AT4G37530.2 39.906 213 119 4 87 291 71 282 7.00e-45 155
Msa0085670 AT2G37130.2 33.591 259 162 5 86 338 42 296 1.50e-44 155
Msa0085670 AT5G64100.1 38.846 260 142 7 87 338 81 331 1.94e-44 156
Msa0085670 AT2G37130.1 33.591 259 162 5 86 338 73 327 3.11e-44 155
Msa0085670 AT1G30870.1 36.431 269 150 9 84 344 94 349 3.68e-43 153
Msa0085670 AT1G77100.1 36.822 258 151 8 87 338 68 319 2.25e-42 150
Msa0085670 AT4G25980.1 34.981 263 154 6 87 338 70 326 3.78e-41 147
Msa0085670 AT3G17070.2 32.283 254 162 4 89 334 21 272 7.56e-40 142
Msa0085670 AT3G17070.1 32.031 256 164 4 87 334 82 335 9.60e-40 144
Msa0085670 AT5G39580.2 36.129 155 88 4 87 241 69 212 7.16e-21 90.5
Msa0085670 AT4G25980.2 38.095 126 72 3 87 212 115 234 8.98e-20 88.6
Msa0085670 AT2G24800.2 41.129 124 67 3 87 210 75 192 2.52e-17 80.9
Msa0085670 AT4G32320.1 27.700 213 117 7 105 317 144 319 6.40e-11 63.2
Msa0085670 AT3G42570.1 32.407 108 69 3 104 210 43 147 9.49e-11 60.1

Find 80 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGCTGCTATGCTTAAAA+TGG 0.176723 1_2:+65919057 Msa0085670:CDS
TACTTGTAAGAATGTTAATT+TGG 0.190227 1_2:+65919326 Msa0085670:three_prime_UTR
AAGTAGGACAAGTTTGTTTA+AGG 0.199321 1_2:-65918850 None:intergenic
TCACAATGGCTTATGCTTCT+TGG 0.262699 1_2:+65917810 Msa0085670:exon
CTGATGATAGATTCAAGTTT+AGG 0.273776 1_2:-65917958 None:intergenic
TAAGGAACATTATTAGATTC+AGG 0.338942 1_2:-65919254 None:intergenic
CTACTACATCTGTTGGGTTA+AGG 0.349085 1_2:-65918724 None:intergenic
ATTGTTGTTGTGTTGCTTGA+TGG 0.350284 1_2:-65918677 None:intergenic
ATAGTCCCTTCCCAATGGTT+TGG 0.364246 1_2:-65917909 None:intergenic
TTGTGATGGATCAGTATTAT+TGG 0.364319 1_2:+65918295 Msa0085670:CDS
CACCACCAAACTTGAGTCTT+AGG 0.384280 1_2:+65918351 Msa0085670:CDS
GTAAAACTAACCTCTTGAAA+AGG 0.408934 1_2:-65919232 None:intergenic
ATTAGCACGAATTTCTCCTT+TGG 0.416931 1_2:-65919104 None:intergenic
TTCGTGCTAATTGCTCTGTT+AGG 0.418468 1_2:+65919116 Msa0085670:CDS
GGTGGTGCATCTTTCTCACT+TGG 0.424168 1_2:-65918335 None:intergenic
AAAGGTAAGGATTAGAAGAA+AGG 0.436359 1_2:-65917849 None:intergenic
GTATTTGTTATCAAATTTGT+TGG 0.437290 1_2:-65918912 None:intergenic
TATTTGTTATCAAATTTGTT+GGG 0.439495 1_2:-65918911 None:intergenic
TCTTATCAGTGTATAAATCT+TGG 0.449979 1_2:-65918976 None:intergenic
CCATGACAGGGTCTTGTGTT+GGG 0.457865 1_2:-65918808 None:intergenic
TCCATGACAGGGTCTTGTGT+TGG 0.462204 1_2:-65918809 None:intergenic
AAGATTTATACACTGATAAG+AGG 0.464368 1_2:+65918978 Msa0085670:CDS
TTAGAAATGTGATAACTACT+AGG 0.473160 1_2:+65919196 Msa0085670:exon
GATGATGTTGGTCAAGCTGC+TGG 0.480697 1_2:+65918006 Msa0085670:CDS
TAAAACTAACCTCTTGAAAA+GGG 0.486235 1_2:-65919231 None:intergenic
TAATCCATCTCTCCTTCCTA+AGG 0.488025 1_2:-65918612 None:intergenic
AACTGTGGTGTTATCAGTGT+TGG 0.488314 1_2:-65918873 None:intergenic
GTTGCTGCTATGCTTAAAAT+GGG 0.493223 1_2:+65919058 Msa0085670:CDS
TCGGTCCTAAGACTCAAGTT+TGG 0.499279 1_2:-65918356 None:intergenic
CAAATCTTTCCTTACTTCTG+TGG 0.502716 1_2:+65919150 Msa0085670:CDS
ATGAAAATGCAGGGTTGTGA+TGG 0.506653 1_2:+65918281 Msa0085670:intron
CATCTTCCACCACAGAAGTA+AGG 0.507687 1_2:-65919159 None:intergenic
GAAGCACAAGCCAAACCATT+GGG 0.508056 1_2:+65917899 Msa0085670:CDS
GATGTGGGAAAATGAAAGAA+AGG 0.511303 1_2:-65917867 None:intergenic
ACTTCCATGACTGCTTGTCC+AGG 0.511431 1_2:+65918043 Msa0085670:CDS
CTCTCAGGTGGACACACAAT+AGG 0.513881 1_2:+65918749 Msa0085670:CDS
GCTTATGCTTCTTGGAGGAG+TGG 0.520217 1_2:+65917818 Msa0085670:CDS
AACAGCATCACGAGCGGCAA+TGG 0.523212 1_2:-65918459 None:intergenic
ATCTTTCCTTACTTCTGTGG+TGG 0.523496 1_2:+65919153 Msa0085670:CDS
TGGATTAACATTTGCAACAC+AGG 0.523958 1_2:+65918628 Msa0085670:CDS
CCAGACTACAAAATTCCCTT+AGG 0.524500 1_2:+65918596 Msa0085670:CDS
AAGATCTTCAATGATGATGT+TGG 0.524826 1_2:+65917994 Msa0085670:CDS
ACAGATGTAGTAGCACTCTC+AGG 0.527358 1_2:+65918734 Msa0085670:CDS
TGTTGATCTTATGAACCGTC+AGG 0.528938 1_2:+65918937 Msa0085670:CDS
GTCCTAAGACTCAAGTTTGG+TGG 0.530334 1_2:-65918353 None:intergenic
TGCTTCAGACACTTTGATGT+GGG 0.540351 1_2:-65917882 None:intergenic
CAATGGCTTATGCTTCTTGG+AGG 0.541632 1_2:+65917813 Msa0085670:exon
TGAAGCACAAGCCAAACCAT+TGG 0.546424 1_2:+65917898 Msa0085670:CDS
GTGCTTCAGACACTTTGATG+TGG 0.547576 1_2:-65917883 None:intergenic
CCTTACGTGTACTATAGTAA+TGG 0.549546 1_2:+65919272 Msa0085670:three_prime_UTR
CCATTACTATAGTACACGTA+AGG 0.551567 1_2:-65919272 None:intergenic
GGGAAAATGAAAGAAAGGTA+AGG 0.553697 1_2:-65917862 None:intergenic
AGAGTGCTACTACATCTGTT+GGG 0.556560 1_2:-65918730 None:intergenic
GAGAGTGCTACTACATCTGT+TGG 0.562833 1_2:-65918731 None:intergenic
TTTACCTGGACAAGCAGTCA+TGG 0.563561 1_2:-65918047 None:intergenic
CCTGTCATGGACAAAACCTA+TGG 0.564791 1_2:+65918821 Msa0085670:CDS
ATACACTGATAAGAGGACTA+AGG 0.565750 1_2:+65918985 Msa0085670:CDS
GTGTTATCAGTGTTGGAAGT+AGG 0.569777 1_2:-65918866 None:intergenic
CCGTCAGGCTCTCTTTACTT+CGG 0.573471 1_2:+65918952 Msa0085670:CDS
ATTCCCTTAGGAAGGAGAGA+TGG 0.574750 1_2:+65918608 Msa0085670:CDS
CACAAGCCAAACCATTGGGA+AGG 0.575921 1_2:+65917903 Msa0085670:CDS
TACTTCTGTGGTGGAAGATG+CGG 0.576534 1_2:+65919162 Msa0085670:CDS
ACTACAAAATTCCCTTAGGA+AGG 0.582731 1_2:+65918600 Msa0085670:CDS
CCATGATAGTCCCTTCCCAA+TGG 0.590397 1_2:-65917914 None:intergenic
AATCCATCTCTCCTTCCTAA+GGG 0.592767 1_2:-65918611 None:intergenic
GATGGATCAGTATTATTGGA+TGG 0.596952 1_2:+65918299 Msa0085670:CDS
TTCTGTGGTGGAAGATGCGG+TGG 0.600054 1_2:+65919165 Msa0085670:CDS
GTTGTGTTGCTTGATGGTGA+AGG 0.611201 1_2:-65918671 None:intergenic
ACAAGCAGTCATGGAAGTGA+AGG 0.611821 1_2:-65918038 None:intergenic
CCCAACACAAGACCCTGTCA+TGG 0.624304 1_2:+65918808 Msa0085670:CDS
CCATTGGGAAGGGACTATCA+TGG 0.637705 1_2:+65917914 Msa0085670:CDS
ACAAGCCAAACCATTGGGAA+GGG 0.640643 1_2:+65917904 Msa0085670:CDS
TTAATATCTACATATCACAA+TGG 0.641022 1_2:+65917796 None:intergenic
GATGTAGTAGCACTCTCAGG+TGG 0.643147 1_2:+65918737 Msa0085670:CDS
ACTACGAATATCTAGAACTG+TGG 0.661587 1_2:-65918888 None:intergenic
ATTAACATTTGCAACACAGG+AGG 0.668426 1_2:+65918631 Msa0085670:CDS
CCGAAGTAAAGAGAGCCTGA+CGG 0.686185 1_2:-65918952 None:intergenic
AGTAATGTAGAGAAGCAGTG+TGG 0.694261 1_2:+65918407 Msa0085670:CDS
TAGGAAAACAGCATCACGAG+CGG 0.695239 1_2:-65918465 None:intergenic
AAACTTGAGTCTTAGGACCG+AGG 0.714645 1_2:+65918358 Msa0085670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATTTGGTTATTTA+TGG + chr1_2:65918249-65918268 Msa0085670:intron 10.0%
!! TTATTATATTTGGTTATTTA+TGG + chr1_2:65918249-65918268 Msa0085670:intron 10.0%
!! AACTATAAACAATACTAATT+AGG + chr1_2:65918111-65918130 Msa0085670:intron 15.0%
!! AAATCCAAACTTATTATATT+TGG + chr1_2:65918239-65918258 Msa0085670:intron 15.0%
!! ATAACCAAATATAATAAGTT+TGG - chr1_2:65918246-65918265 None:intergenic 15.0%
!! ATTAAAAAAATGAAAATGCA+GGG + chr1_2:65918272-65918291 Msa0085670:intron 15.0%
!!! TATTTGTTATCAAATTTGTT+GGG - chr1_2:65918914-65918933 None:intergenic 15.0%
!! AACTATAAACAATACTAATT+AGG + chr1_2:65918111-65918130 Msa0085670:intron 15.0%
!! AAATCCAAACTTATTATATT+TGG + chr1_2:65918239-65918258 Msa0085670:intron 15.0%
!! ATAACCAAATATAATAAGTT+TGG - chr1_2:65918246-65918265 None:intergenic 15.0%
!! ATTAAAAAAATGAAAATGCA+GGG + chr1_2:65918272-65918291 Msa0085670:intron 15.0%
!!! TATTTGTTATCAAATTTGTT+GGG - chr1_2:65918914-65918933 None:intergenic 15.0%
!!! TAATATTTTATTGACACATG+TGG + chr1_2:65918175-65918194 Msa0085670:intron 20.0%
!! GATTAAAAAAATGAAAATGC+AGG + chr1_2:65918271-65918290 Msa0085670:intron 20.0%
!!! AAGTTTTTCGATTATTTTGA+AGG - chr1_2:65918384-65918403 None:intergenic 20.0%
!! AAAATATAGGTTTACTTACT+AGG - chr1_2:65918487-65918506 None:intergenic 20.0%
!!! GTATTTGTTATCAAATTTGT+TGG - chr1_2:65918915-65918934 None:intergenic 20.0%
!! TACTTGTAAGAATGTTAATT+TGG + chr1_2:65919326-65919345 Msa0085670:three_prime_UTR 20.0%
!!! TAATATTTTATTGACACATG+TGG + chr1_2:65918175-65918194 Msa0085670:intron 20.0%
!! GATTAAAAAAATGAAAATGC+AGG + chr1_2:65918271-65918290 Msa0085670:intron 20.0%
!!! AAGTTTTTCGATTATTTTGA+AGG - chr1_2:65918384-65918403 None:intergenic 20.0%
!! AAAATATAGGTTTACTTACT+AGG - chr1_2:65918487-65918506 None:intergenic 20.0%
!!! GTATTTGTTATCAAATTTGT+TGG - chr1_2:65918915-65918934 None:intergenic 20.0%
!! TACTTGTAAGAATGTTAATT+TGG + chr1_2:65919326-65919345 Msa0085670:three_prime_UTR 20.0%
!! ATAGAAGCTGTAAATTTTCA+TGG - chr1_2:65918154-65918173 None:intergenic 25.0%
! TCTTATCAGTGTATAAATCT+TGG - chr1_2:65918979-65918998 None:intergenic 25.0%
! AAGATTTATACACTGATAAG+AGG + chr1_2:65918978-65918997 Msa0085670:CDS 25.0%
! TTAGAAATGTGATAACTACT+AGG + chr1_2:65919196-65919215 Msa0085670:exon 25.0%
! TAAAACTAACCTCTTGAAAA+GGG - chr1_2:65919234-65919253 None:intergenic 25.0%
! TAAGGAACATTATTAGATTC+AGG - chr1_2:65919257-65919276 None:intergenic 25.0%
!! ATAGAAGCTGTAAATTTTCA+TGG - chr1_2:65918154-65918173 None:intergenic 25.0%
! TCTTATCAGTGTATAAATCT+TGG - chr1_2:65918979-65918998 None:intergenic 25.0%
! AAGATTTATACACTGATAAG+AGG + chr1_2:65918978-65918997 Msa0085670:CDS 25.0%
! TTAGAAATGTGATAACTACT+AGG + chr1_2:65919196-65919215 Msa0085670:exon 25.0%
! TAAAACTAACCTCTTGAAAA+GGG - chr1_2:65919234-65919253 None:intergenic 25.0%
! TAAGGAACATTATTAGATTC+AGG - chr1_2:65919257-65919276 None:intergenic 25.0%
AAAGGTAAGGATTAGAAGAA+AGG - chr1_2:65917852-65917871 None:intergenic 30.0%
! CTGATGATAGATTCAAGTTT+AGG - chr1_2:65917961-65917980 None:intergenic 30.0%
! AAGATCTTCAATGATGATGT+TGG + chr1_2:65917994-65918013 Msa0085670:CDS 30.0%
ATACATGAATTGTGTTTACC+TGG - chr1_2:65918064-65918083 None:intergenic 30.0%
TGAATCTGTTTAGCTAACTT+TGG + chr1_2:65918216-65918235 Msa0085670:intron 30.0%
!! TTGTGATGGATCAGTATTAT+TGG + chr1_2:65918295-65918314 Msa0085670:CDS 30.0%
CCAACTTCTCACAAAAATAT+AGG - chr1_2:65918500-65918519 None:intergenic 30.0%
!! CCTATATTTTTGTGAGAAGT+TGG + chr1_2:65918497-65918516 Msa0085670:intron 30.0%
!! TTGTTTAAGGTTTTTGCCAT+AGG - chr1_2:65918840-65918859 None:intergenic 30.0%
! AAGTAGGACAAGTTTGTTTA+AGG - chr1_2:65918853-65918872 None:intergenic 30.0%
!! TATTTGTTTCCCTTTTCAAG+AGG + chr1_2:65919222-65919241 Msa0085670:three_prime_UTR 30.0%
GTAAAACTAACCTCTTGAAA+AGG - chr1_2:65919235-65919254 None:intergenic 30.0%
AAAGGTAAGGATTAGAAGAA+AGG - chr1_2:65917852-65917871 None:intergenic 30.0%
! CTGATGATAGATTCAAGTTT+AGG - chr1_2:65917961-65917980 None:intergenic 30.0%
! AAGATCTTCAATGATGATGT+TGG + chr1_2:65917994-65918013 Msa0085670:CDS 30.0%
ATACATGAATTGTGTTTACC+TGG - chr1_2:65918064-65918083 None:intergenic 30.0%
TGAATCTGTTTAGCTAACTT+TGG + chr1_2:65918216-65918235 Msa0085670:intron 30.0%
!! TTGTGATGGATCAGTATTAT+TGG + chr1_2:65918295-65918314 Msa0085670:CDS 30.0%
CCAACTTCTCACAAAAATAT+AGG - chr1_2:65918500-65918519 None:intergenic 30.0%
!! CCTATATTTTTGTGAGAAGT+TGG + chr1_2:65918497-65918516 Msa0085670:intron 30.0%
!! TTGTTTAAGGTTTTTGCCAT+AGG - chr1_2:65918840-65918859 None:intergenic 30.0%
! AAGTAGGACAAGTTTGTTTA+AGG - chr1_2:65918853-65918872 None:intergenic 30.0%
!! TATTTGTTTCCCTTTTCAAG+AGG + chr1_2:65919222-65919241 Msa0085670:three_prime_UTR 30.0%
GTAAAACTAACCTCTTGAAA+AGG - chr1_2:65919235-65919254 None:intergenic 30.0%
GGGAAAATGAAAGAAAGGTA+AGG - chr1_2:65917865-65917884 None:intergenic 35.0%
GATGTGGGAAAATGAAAGAA+AGG - chr1_2:65917870-65917889 None:intergenic 35.0%
! AGGACATTTTGAATCGTAGA+AGG - chr1_2:65917941-65917960 None:intergenic 35.0%
GATGGATCAGTATTATTGGA+TGG + chr1_2:65918299-65918318 Msa0085670:CDS 35.0%
!!! TTCGATTATTTTGAAGGCCT+CGG - chr1_2:65918378-65918397 None:intergenic 35.0%
!! CATGTTTTTACATTGCAGTC+AGG + chr1_2:65918569-65918588 Msa0085670:intron 35.0%
ACTACAAAATTCCCTTAGGA+AGG + chr1_2:65918600-65918619 Msa0085670:CDS 35.0%
TGGATTAACATTTGCAACAC+AGG + chr1_2:65918628-65918647 Msa0085670:CDS 35.0%
ATTAACATTTGCAACACAGG+AGG + chr1_2:65918631-65918650 Msa0085670:CDS 35.0%
!! ATTGTTGTTGTGTTGCTTGA+TGG - chr1_2:65918680-65918699 None:intergenic 35.0%
!!! TGTTGGGTTAAGGTTTTTTG+TGG - chr1_2:65918717-65918736 None:intergenic 35.0%
! ACTACGAATATCTAGAACTG+TGG - chr1_2:65918891-65918910 None:intergenic 35.0%
ATACACTGATAAGAGGACTA+AGG + chr1_2:65918985-65919004 Msa0085670:CDS 35.0%
TGTTGCTGCTATGCTTAAAA+TGG + chr1_2:65919057-65919076 Msa0085670:CDS 35.0%
GTTGCTGCTATGCTTAAAAT+GGG + chr1_2:65919058-65919077 Msa0085670:CDS 35.0%
!!! AATGTTTTGACTGGAACCAA+AGG + chr1_2:65919088-65919107 Msa0085670:CDS 35.0%
ATTAGCACGAATTTCTCCTT+TGG - chr1_2:65919107-65919126 None:intergenic 35.0%
CAAATCTTTCCTTACTTCTG+TGG + chr1_2:65919150-65919169 Msa0085670:CDS 35.0%
CCATTACTATAGTACACGTA+AGG - chr1_2:65919275-65919294 None:intergenic 35.0%
CCTTACGTGTACTATAGTAA+TGG + chr1_2:65919272-65919291 Msa0085670:three_prime_UTR 35.0%
GGGAAAATGAAAGAAAGGTA+AGG - chr1_2:65917865-65917884 None:intergenic 35.0%
GATGTGGGAAAATGAAAGAA+AGG - chr1_2:65917870-65917889 None:intergenic 35.0%
! AGGACATTTTGAATCGTAGA+AGG - chr1_2:65917941-65917960 None:intergenic 35.0%
GATGGATCAGTATTATTGGA+TGG + chr1_2:65918299-65918318 Msa0085670:CDS 35.0%
!!! TTCGATTATTTTGAAGGCCT+CGG - chr1_2:65918378-65918397 None:intergenic 35.0%
!! CATGTTTTTACATTGCAGTC+AGG + chr1_2:65918569-65918588 Msa0085670:intron 35.0%
ACTACAAAATTCCCTTAGGA+AGG + chr1_2:65918600-65918619 Msa0085670:CDS 35.0%
TGGATTAACATTTGCAACAC+AGG + chr1_2:65918628-65918647 Msa0085670:CDS 35.0%
ATTAACATTTGCAACACAGG+AGG + chr1_2:65918631-65918650 Msa0085670:CDS 35.0%
!! ATTGTTGTTGTGTTGCTTGA+TGG - chr1_2:65918680-65918699 None:intergenic 35.0%
!!! TGTTGGGTTAAGGTTTTTTG+TGG - chr1_2:65918717-65918736 None:intergenic 35.0%
! ACTACGAATATCTAGAACTG+TGG - chr1_2:65918891-65918910 None:intergenic 35.0%
ATACACTGATAAGAGGACTA+AGG + chr1_2:65918985-65919004 Msa0085670:CDS 35.0%
TGTTGCTGCTATGCTTAAAA+TGG + chr1_2:65919057-65919076 Msa0085670:CDS 35.0%
GTTGCTGCTATGCTTAAAAT+GGG + chr1_2:65919058-65919077 Msa0085670:CDS 35.0%
!!! AATGTTTTGACTGGAACCAA+AGG + chr1_2:65919088-65919107 Msa0085670:CDS 35.0%
ATTAGCACGAATTTCTCCTT+TGG - chr1_2:65919107-65919126 None:intergenic 35.0%
CAAATCTTTCCTTACTTCTG+TGG + chr1_2:65919150-65919169 Msa0085670:CDS 35.0%
CCATTACTATAGTACACGTA+AGG - chr1_2:65919275-65919294 None:intergenic 35.0%
CCTTACGTGTACTATAGTAA+TGG + chr1_2:65919272-65919291 Msa0085670:three_prime_UTR 35.0%
TCACAATGGCTTATGCTTCT+TGG + chr1_2:65917810-65917829 Msa0085670:exon 40.0%
TGCTTCAGACACTTTGATGT+GGG - chr1_2:65917885-65917904 None:intergenic 40.0%
ATGAAAATGCAGGGTTGTGA+TGG + chr1_2:65918281-65918300 Msa0085670:intron 40.0%
AGTAATGTAGAGAAGCAGTG+TGG + chr1_2:65918407-65918426 Msa0085670:CDS 40.0%
! GTTTTTACATTGCAGTCAGG+AGG + chr1_2:65918572-65918591 Msa0085670:intron 40.0%
! CCTAAGGGAATTTTGTAGTC+TGG - chr1_2:65918599-65918618 None:intergenic 40.0%
CCAGACTACAAAATTCCCTT+AGG + chr1_2:65918596-65918615 Msa0085670:CDS 40.0%
AATCCATCTCTCCTTCCTAA+GGG - chr1_2:65918614-65918633 None:intergenic 40.0%
TAATCCATCTCTCCTTCCTA+AGG - chr1_2:65918615-65918634 None:intergenic 40.0%
CTACTACATCTGTTGGGTTA+AGG - chr1_2:65918727-65918746 None:intergenic 40.0%
! AGAGTGCTACTACATCTGTT+GGG - chr1_2:65918733-65918752 None:intergenic 40.0%
!! CATAGGTTTTGTCCATGACA+GGG - chr1_2:65918823-65918842 None:intergenic 40.0%
! GTGTTATCAGTGTTGGAAGT+AGG - chr1_2:65918869-65918888 None:intergenic 40.0%
!! AACTGTGGTGTTATCAGTGT+TGG - chr1_2:65918876-65918895 None:intergenic 40.0%
TGTTGATCTTATGAACCGTC+AGG + chr1_2:65918937-65918956 Msa0085670:CDS 40.0%
!!! GGACAGCTTAATGTTTTGAC+TGG + chr1_2:65919079-65919098 Msa0085670:CDS 40.0%
TTCGTGCTAATTGCTCTGTT+AGG + chr1_2:65919116-65919135 Msa0085670:CDS 40.0%
ATCTTTCCTTACTTCTGTGG+TGG + chr1_2:65919153-65919172 Msa0085670:CDS 40.0%
TCACAATGGCTTATGCTTCT+TGG + chr1_2:65917810-65917829 Msa0085670:exon 40.0%
TGCTTCAGACACTTTGATGT+GGG - chr1_2:65917885-65917904 None:intergenic 40.0%
ATGAAAATGCAGGGTTGTGA+TGG + chr1_2:65918281-65918300 Msa0085670:intron 40.0%
AGTAATGTAGAGAAGCAGTG+TGG + chr1_2:65918407-65918426 Msa0085670:CDS 40.0%
! GTTTTTACATTGCAGTCAGG+AGG + chr1_2:65918572-65918591 Msa0085670:intron 40.0%
! CCTAAGGGAATTTTGTAGTC+TGG - chr1_2:65918599-65918618 None:intergenic 40.0%
CCAGACTACAAAATTCCCTT+AGG + chr1_2:65918596-65918615 Msa0085670:CDS 40.0%
AATCCATCTCTCCTTCCTAA+GGG - chr1_2:65918614-65918633 None:intergenic 40.0%
TAATCCATCTCTCCTTCCTA+AGG - chr1_2:65918615-65918634 None:intergenic 40.0%
CTACTACATCTGTTGGGTTA+AGG - chr1_2:65918727-65918746 None:intergenic 40.0%
! AGAGTGCTACTACATCTGTT+GGG - chr1_2:65918733-65918752 None:intergenic 40.0%
!! CATAGGTTTTGTCCATGACA+GGG - chr1_2:65918823-65918842 None:intergenic 40.0%
! GTGTTATCAGTGTTGGAAGT+AGG - chr1_2:65918869-65918888 None:intergenic 40.0%
!! AACTGTGGTGTTATCAGTGT+TGG - chr1_2:65918876-65918895 None:intergenic 40.0%
TGTTGATCTTATGAACCGTC+AGG + chr1_2:65918937-65918956 Msa0085670:CDS 40.0%
!!! GGACAGCTTAATGTTTTGAC+TGG + chr1_2:65919079-65919098 Msa0085670:CDS 40.0%
TTCGTGCTAATTGCTCTGTT+AGG + chr1_2:65919116-65919135 Msa0085670:CDS 40.0%
ATCTTTCCTTACTTCTGTGG+TGG + chr1_2:65919153-65919172 Msa0085670:CDS 40.0%
CAATGGCTTATGCTTCTTGG+AGG + chr1_2:65917813-65917832 Msa0085670:exon 45.0%
GTGCTTCAGACACTTTGATG+TGG - chr1_2:65917886-65917905 None:intergenic 45.0%
TGAAGCACAAGCCAAACCAT+TGG + chr1_2:65917898-65917917 Msa0085670:CDS 45.0%
GAAGCACAAGCCAAACCATT+GGG + chr1_2:65917899-65917918 Msa0085670:CDS 45.0%
ACAAGCCAAACCATTGGGAA+GGG + chr1_2:65917904-65917923 Msa0085670:CDS 45.0%
ATAGTCCCTTCCCAATGGTT+TGG - chr1_2:65917912-65917931 None:intergenic 45.0%
ACAAGCAGTCATGGAAGTGA+AGG - chr1_2:65918041-65918060 None:intergenic 45.0%
TTTACCTGGACAAGCAGTCA+TGG - chr1_2:65918050-65918069 None:intergenic 45.0%
CACCACCAAACTTGAGTCTT+AGG + chr1_2:65918351-65918370 Msa0085670:CDS 45.0%
GTCCTAAGACTCAAGTTTGG+TGG - chr1_2:65918356-65918375 None:intergenic 45.0%
TCGGTCCTAAGACTCAAGTT+TGG - chr1_2:65918359-65918378 None:intergenic 45.0%
AAACTTGAGTCTTAGGACCG+AGG + chr1_2:65918358-65918377 Msa0085670:CDS 45.0%
TAGGAAAACAGCATCACGAG+CGG - chr1_2:65918468-65918487 None:intergenic 45.0%
ATTCCCTTAGGAAGGAGAGA+TGG + chr1_2:65918608-65918627 Msa0085670:CDS 45.0%
!! GTTGTGTTGCTTGATGGTGA+AGG - chr1_2:65918674-65918693 None:intergenic 45.0%
!! GAGAGTGCTACTACATCTGT+TGG - chr1_2:65918734-65918753 None:intergenic 45.0%
ACAGATGTAGTAGCACTCTC+AGG + chr1_2:65918734-65918753 Msa0085670:CDS 45.0%
!! CCATAGGTTTTGTCCATGAC+AGG - chr1_2:65918824-65918843 None:intergenic 45.0%
CCTGTCATGGACAAAACCTA+TGG + chr1_2:65918821-65918840 Msa0085670:CDS 45.0%
CATCTTCCACCACAGAAGTA+AGG - chr1_2:65919162-65919181 None:intergenic 45.0%
! TACTTCTGTGGTGGAAGATG+CGG + chr1_2:65919162-65919181 Msa0085670:CDS 45.0%
CAATGGCTTATGCTTCTTGG+AGG + chr1_2:65917813-65917832 Msa0085670:exon 45.0%
GTGCTTCAGACACTTTGATG+TGG - chr1_2:65917886-65917905 None:intergenic 45.0%
TGAAGCACAAGCCAAACCAT+TGG + chr1_2:65917898-65917917 Msa0085670:CDS 45.0%
GAAGCACAAGCCAAACCATT+GGG + chr1_2:65917899-65917918 Msa0085670:CDS 45.0%
ACAAGCCAAACCATTGGGAA+GGG + chr1_2:65917904-65917923 Msa0085670:CDS 45.0%
ATAGTCCCTTCCCAATGGTT+TGG - chr1_2:65917912-65917931 None:intergenic 45.0%
ACAAGCAGTCATGGAAGTGA+AGG - chr1_2:65918041-65918060 None:intergenic 45.0%
TTTACCTGGACAAGCAGTCA+TGG - chr1_2:65918050-65918069 None:intergenic 45.0%
CACCACCAAACTTGAGTCTT+AGG + chr1_2:65918351-65918370 Msa0085670:CDS 45.0%
GTCCTAAGACTCAAGTTTGG+TGG - chr1_2:65918356-65918375 None:intergenic 45.0%
TCGGTCCTAAGACTCAAGTT+TGG - chr1_2:65918359-65918378 None:intergenic 45.0%
AAACTTGAGTCTTAGGACCG+AGG + chr1_2:65918358-65918377 Msa0085670:CDS 45.0%
TAGGAAAACAGCATCACGAG+CGG - chr1_2:65918468-65918487 None:intergenic 45.0%
ATTCCCTTAGGAAGGAGAGA+TGG + chr1_2:65918608-65918627 Msa0085670:CDS 45.0%
!! GTTGTGTTGCTTGATGGTGA+AGG - chr1_2:65918674-65918693 None:intergenic 45.0%
!! GAGAGTGCTACTACATCTGT+TGG - chr1_2:65918734-65918753 None:intergenic 45.0%
ACAGATGTAGTAGCACTCTC+AGG + chr1_2:65918734-65918753 Msa0085670:CDS 45.0%
!! CCATAGGTTTTGTCCATGAC+AGG - chr1_2:65918824-65918843 None:intergenic 45.0%
CCTGTCATGGACAAAACCTA+TGG + chr1_2:65918821-65918840 Msa0085670:CDS 45.0%
CATCTTCCACCACAGAAGTA+AGG - chr1_2:65919162-65919181 None:intergenic 45.0%
! TACTTCTGTGGTGGAAGATG+CGG + chr1_2:65919162-65919181 Msa0085670:CDS 45.0%
GCTTATGCTTCTTGGAGGAG+TGG + chr1_2:65917818-65917837 Msa0085670:CDS 50.0%
CACAAGCCAAACCATTGGGA+AGG + chr1_2:65917903-65917922 Msa0085670:CDS 50.0%
CCATGATAGTCCCTTCCCAA+TGG - chr1_2:65917917-65917936 None:intergenic 50.0%
CCATTGGGAAGGGACTATCA+TGG + chr1_2:65917914-65917933 Msa0085670:CDS 50.0%
GATGATGTTGGTCAAGCTGC+TGG + chr1_2:65918006-65918025 Msa0085670:CDS 50.0%
ACTTCCATGACTGCTTGTCC+AGG + chr1_2:65918043-65918062 Msa0085670:CDS 50.0%
GGTGGTGCATCTTTCTCACT+TGG - chr1_2:65918338-65918357 None:intergenic 50.0%
GATGTAGTAGCACTCTCAGG+TGG + chr1_2:65918737-65918756 Msa0085670:CDS 50.0%
CTCTCAGGTGGACACACAAT+AGG + chr1_2:65918749-65918768 Msa0085670:CDS 50.0%
! CCATGACAGGGTCTTGTGTT+GGG - chr1_2:65918811-65918830 None:intergenic 50.0%
! TCCATGACAGGGTCTTGTGT+TGG - chr1_2:65918812-65918831 None:intergenic 50.0%
CCGAAGTAAAGAGAGCCTGA+CGG - chr1_2:65918955-65918974 None:intergenic 50.0%
CCGTCAGGCTCTCTTTACTT+CGG + chr1_2:65918952-65918971 Msa0085670:CDS 50.0%
GCTTATGCTTCTTGGAGGAG+TGG + chr1_2:65917818-65917837 Msa0085670:CDS 50.0%
CACAAGCCAAACCATTGGGA+AGG + chr1_2:65917903-65917922 Msa0085670:CDS 50.0%
CCATGATAGTCCCTTCCCAA+TGG - chr1_2:65917917-65917936 None:intergenic 50.0%
CCATTGGGAAGGGACTATCA+TGG + chr1_2:65917914-65917933 Msa0085670:CDS 50.0%
GATGATGTTGGTCAAGCTGC+TGG + chr1_2:65918006-65918025 Msa0085670:CDS 50.0%
ACTTCCATGACTGCTTGTCC+AGG + chr1_2:65918043-65918062 Msa0085670:CDS 50.0%
GGTGGTGCATCTTTCTCACT+TGG - chr1_2:65918338-65918357 None:intergenic 50.0%
GATGTAGTAGCACTCTCAGG+TGG + chr1_2:65918737-65918756 Msa0085670:CDS 50.0%
CTCTCAGGTGGACACACAAT+AGG + chr1_2:65918749-65918768 Msa0085670:CDS 50.0%
! CCATGACAGGGTCTTGTGTT+GGG - chr1_2:65918811-65918830 None:intergenic 50.0%
! TCCATGACAGGGTCTTGTGT+TGG - chr1_2:65918812-65918831 None:intergenic 50.0%
CCGAAGTAAAGAGAGCCTGA+CGG - chr1_2:65918955-65918974 None:intergenic 50.0%
CCGTCAGGCTCTCTTTACTT+CGG + chr1_2:65918952-65918971 Msa0085670:CDS 50.0%
AACAGCATCACGAGCGGCAA+TGG - chr1_2:65918462-65918481 None:intergenic 55.0%
CCCAACACAAGACCCTGTCA+TGG + chr1_2:65918808-65918827 Msa0085670:CDS 55.0%
! TTCTGTGGTGGAAGATGCGG+TGG + chr1_2:65919165-65919184 Msa0085670:CDS 55.0%
AACAGCATCACGAGCGGCAA+TGG - chr1_2:65918462-65918481 None:intergenic 55.0%
CCCAACACAAGACCCTGTCA+TGG + chr1_2:65918808-65918827 Msa0085670:CDS 55.0%
! TTCTGTGGTGGAAGATGCGG+TGG + chr1_2:65919165-65919184 Msa0085670:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 65917809 65919376 65917809 ID=Msa0085670;Name=Msa0085670
chr1_2 mRNA 65917809 65919376 65917809 ID=Msa0085670-mRNA-1;Parent=Msa0085670;Name=Msa0085670-mRNA-1;_AED=0.05;_eAED=0.05;_QI=6|0.33|0.75|0.75|0.33|0.5|4|169|359
chr1_2 exon 65917809 65918064 65917809 ID=Msa0085670-mRNA-1:exon:22272;Parent=Msa0085670-mRNA-1
chr1_2 exon 65918133 65918146 65918133 ID=Msa0085670-mRNA-1:exon:22273;Parent=Msa0085670-mRNA-1
chr1_2 exon 65918293 65918487 65918293 ID=Msa0085670-mRNA-1:exon:22274;Parent=Msa0085670-mRNA-1
chr1_2 exon 65918587 65919376 65918587 ID=Msa0085670-mRNA-1:exon:22275;Parent=Msa0085670-mRNA-1
chr1_2 five_prime_UTR 65917809 65917814 65917809 ID=Msa0085670-mRNA-1:five_prime_utr;Parent=Msa0085670-mRNA-1
chr1_2 CDS 65917815 65918064 65917815 ID=Msa0085670-mRNA-1:cds;Parent=Msa0085670-mRNA-1
chr1_2 CDS 65918133 65918146 65918133 ID=Msa0085670-mRNA-1:cds;Parent=Msa0085670-mRNA-1
chr1_2 CDS 65918293 65918487 65918293 ID=Msa0085670-mRNA-1:cds;Parent=Msa0085670-mRNA-1
chr1_2 CDS 65918587 65919207 65918587 ID=Msa0085670-mRNA-1:cds;Parent=Msa0085670-mRNA-1
chr1_2 three_prime_UTR 65919208 65919376 65919208 ID=Msa0085670-mRNA-1:three_prime_utr;Parent=Msa0085670-mRNA-1
Gene Sequence

>Msa0085670

ATGGCTTATGCTTCTTGGAGGAGTGGTTTCTTCACCTTTCTTCTAATCCTTACCTTTCTTTCATTTTCCCACATCAAAGTGTCTGAAGCACAAGCCAAACCATTGGGAAGGGACTATCATGGACCTTCTACGATTCAAAATGTCCTAAACTTGAATCTATCATCAGAAAAGAGCTCAAAAAGATCTTCAATGATGATGTTGGTCAAGCTGCTGGCTTGCTTCGCCTTCACTTCCATGACTGCTTGTCCAGGCATTACAATGCGTGGTTGTGATGGATCAGTATTATTGGATGGATCAGCAAGTGAGCCAAGTGAGAAAGATGCACCACCAAACTTGAGTCTTAGGACCGAGGCCTTCAAAATAATCGAAAAACTTCGTAGTAATGTAGAGAAGCAGTGTGGAAGAATCGTCTCTTGTGCTGATATCACTGCCATTGCCGCTCGTGATGCTGTTTTCCTATCAGGAGGACCAGACTACAAAATTCCCTTAGGAAGGAGAGATGGATTAACATTTGCAACACAGGAGGTAACATTAGACAATCTTCCTTCACCATCAAGCAACACAACAACAATCTTAAACTCACTTGCCACAAAAAACCTTAACCCAACAGATGTAGTAGCACTCTCAGGTGGACACACAATAGGCATAAGTCACTGCTCTTCTTTCATAGACAGACTCTACCCAACACAAGACCCTGTCATGGACAAAACCTATGGCAAAAACCTTAAACAAACTTGTCCTACTTCCAACACTGATAACACCACAGTTCTAGATATTCGTAGTCCCAACAAATTTGATAACAAATACTATGTTGATCTTATGAACCGTCAGGCTCTCTTTACTTCGGACCAAGATTTATACACTGATAAGAGGACTAAGGATATTGTTACTAACTTTGCTGTGAATCAATCTTTGTTCTTTGAGAAATTTGTTGCTGCTATGCTTAAAATGGGACAGCTTAATGTTTTGACTGGAACCAAAGGAGAAATTCGTGCTAATTGCTCTGTTAGGAATAAAAATAGCAAATCTTTCCTTACTTCTGTGGTGGAAGATGCGGTGGAAAGATTTTTAGAAATGTGA
Protein sequence

>Msa0085670

MAYASWRSGFFTFLLILTFLSFSHIKVSEAQAKPLGRDYHGPSTIQNVLNLNLSSEKSSKRSSMMMLVKLLACFAFTSMTACPGITMRGCDGSVLLDGSASEPSEKDAPPNLSLRTEAFKIIEKLRSNVEKQCGRIVSCADITAIAARDAVFLSGGPDYKIPLGRRDGLTFATQEVTLDNLPSPSSNTTTILNSLATKNLNPTDVVALSGGHTIGISHCSSFIDRLYPTQDPVMDKTYGKNLKQTCPTSNTDNTTVLDIRSPNKFDNKYYVDLMNRQALFTSDQDLYTDKRTKDIVTNFAVNQSLFFEKFVAAMLKMGQLNVLTGTKGEIRANCSVRNKNSKSFLTSVVEDAVERFLEM*