AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0174990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174990 MtrunA17_Chr6g0467121 56.571 350 143 4 8 354 41 384 5.00e-143 410
Msa0174990 MtrunA17_Chr6g0467161 52.819 337 153 5 1 336 5 336 4.07e-124 360
Msa0174990 MtrunA17_Chr6g0467251 48.538 342 165 5 1 340 1 333 4.74e-110 325
Msa0174990 MtrunA17_Chr6g0477071 40.120 334 177 6 13 332 14 338 1.27e-86 265
Msa0174990 MtrunA17_Chr2g0292151 45.031 322 164 7 41 352 29 347 1.42e-86 265
Msa0174990 MtrunA17_Chr5g0404721 46.441 295 149 5 45 332 34 326 1.57e-86 264
Msa0174990 MtrunA17_Chr2g0292211 44.444 315 163 6 34 339 33 344 2.20e-85 262
Msa0174990 MtrunA17_Chr3g0128061 46.885 305 149 6 41 336 26 326 4.49e-85 260
Msa0174990 MtrunA17_Chr2g0292271 41.691 343 179 7 19 352 16 346 8.88e-85 260
Msa0174990 MtrunA17_Chr1g0188431 41.781 292 163 4 41 332 39 323 1.32e-83 256
Msa0174990 MtrunA17_Chr8g0372601 44.482 299 157 5 45 336 34 330 2.22e-83 256
Msa0174990 MtrunA17_Chr4g0038161 41.176 340 176 6 1 336 1 320 1.47e-82 254
Msa0174990 MtrunA17_Chr2g0292251 42.724 323 173 6 41 354 28 347 2.15e-82 254
Msa0174990 MtrunA17_Chr1g0194151 45.603 307 153 7 41 336 30 333 2.53e-82 253
Msa0174990 MtrunA17_Chr8g0371381 44.884 303 151 6 41 336 26 319 3.32e-82 253
Msa0174990 MtrunA17_Chr2g0291751 42.398 342 172 8 12 339 3 333 3.62e-82 254
Msa0174990 MtrunA17_Chr2g0292091 42.812 320 171 6 41 351 24 340 4.75e-82 253
Msa0174990 MtrunA17_Chr2g0292241 44.156 308 160 6 41 339 29 333 7.79e-82 253
Msa0174990 MtrunA17_Chr7g0258811 41.945 329 171 7 13 332 50 367 2.29e-81 252
Msa0174990 MtrunA17_Chr4g0038151 43.000 300 164 4 41 336 25 321 2.89e-81 250
Msa0174990 MtrunA17_Chr2g0322861 44.918 305 154 7 41 336 87 386 1.21e-80 251
Msa0174990 MtrunA17_Chr2g0292111 42.857 315 168 6 45 350 33 344 1.21e-80 250
Msa0174990 MtrunA17_Chr2g0299691 41.399 343 175 9 5 336 31 358 1.93e-80 249
Msa0174990 MtrunA17_Chr1g0155611 44.186 301 158 5 40 332 45 343 1.97e-80 249
Msa0174990 MtrunA17_Chr4g0070951 41.813 342 173 8 5 336 4 329 2.64e-80 248
Msa0174990 MtrunA17_Chr4g0013171 39.938 323 186 6 17 336 4 321 5.08e-80 247
Msa0174990 MtrunA17_Chr2g0292101 43.260 319 171 5 41 351 25 341 6.28e-80 248
Msa0174990 MtrunA17_Chr2g0292181 42.532 308 165 6 41 339 38 342 7.38e-80 248
Msa0174990 MtrunA17_Chr1g0194271 44.586 314 153 7 41 336 30 340 1.36e-79 247
Msa0174990 MtrunA17_Chr4g0053871 42.424 297 167 3 41 334 27 322 3.74e-79 245
Msa0174990 MtrunA17_Chr2g0320101 41.980 293 165 4 45 336 29 317 3.78e-79 245
Msa0174990 MtrunA17_Chr5g0405991 42.525 301 157 7 39 332 21 312 4.31e-79 244
Msa0174990 MtrunA17_Chr7g0225221 42.953 298 166 4 41 336 25 320 4.62e-79 245
Msa0174990 MtrunA17_Chr1g0204011 41.231 325 168 7 19 336 14 322 1.13e-78 244
Msa0174990 MtrunA17_Chr4g0038171 42.193 301 166 5 41 336 24 321 2.72e-78 243
Msa0174990 MtrunA17_Chr4g0043391 42.193 301 166 5 41 336 27 324 4.44e-78 242
Msa0174990 MtrunA17_Chr2g0320071 41.297 293 167 4 45 336 29 317 5.35e-78 242
Msa0174990 MtrunA17_Chr7g0254201 42.484 306 167 4 35 337 14 313 5.49e-78 242
Msa0174990 MtrunA17_Chr1g0175561 41.391 302 164 7 41 332 24 322 1.15e-77 241
Msa0174990 MtrunA17_Chr3g0106921 41.250 320 173 4 16 332 9 316 1.39e-77 241
Msa0174990 MtrunA17_Chr1g0164891 43.377 302 159 7 45 336 93 392 2.55e-77 243
Msa0174990 MtrunA17_Chr5g0402571 41.748 309 170 5 35 336 21 326 3.52e-77 240
Msa0174990 MtrunA17_Chr2g0279041 43.506 308 157 6 39 336 25 325 3.73e-77 240
Msa0174990 MtrunA17_Chr3g0114451 40.000 325 182 6 9 332 2 314 6.15e-77 239
Msa0174990 MtrunA17_Chr7g0244461 41.946 298 166 5 41 336 20 312 6.21e-77 239
Msa0174990 MtrunA17_Chr7g0254231 40.878 296 169 3 41 336 22 311 6.21e-77 239
Msa0174990 MtrunA17_Chr4g0024121 42.475 299 162 5 45 336 31 326 7.27e-77 239
Msa0174990 MtrunA17_Chr5g0402581 42.581 310 166 5 35 336 21 326 7.34e-77 239
Msa0174990 MtrunA17_Chr7g0268501 41.515 330 173 7 13 336 5 320 9.85e-77 239
Msa0174990 MtrunA17_Chr7g0268521 41.515 330 173 7 13 336 5 320 9.85e-77 239
Msa0174990 MtrunA17_Chr1g0157051 40.984 305 165 7 37 334 20 316 2.22e-76 238
Msa0174990 MtrunA17_Chr2g0292261 42.368 321 172 7 41 352 29 345 2.41e-76 239
Msa0174990 MtrunA17_Chr2g0320061 41.837 294 165 5 45 336 29 318 4.64e-76 237
Msa0174990 MtrunA17_Chr5g0432441 41.860 301 166 4 41 336 30 326 6.51e-76 237
Msa0174990 MtrunA17_Chr5g0400331 42.997 307 155 9 45 336 30 331 7.80e-76 237
Msa0174990 MtrunA17_Chr5g0401431 40.909 308 171 5 36 334 22 327 8.67e-76 236
Msa0174990 MtrunA17_Chr7g0244451 42.006 319 169 6 16 332 8 312 1.04e-75 236
Msa0174990 MtrunA17_Chr2g0320051 41.497 294 166 5 45 336 29 318 5.68e-75 234
Msa0174990 MtrunA17_Chr4g0013201 43.279 305 166 5 34 336 39 338 5.84e-75 235
Msa0174990 MtrunA17_Chr3g0129051 40.678 295 168 4 41 332 28 318 6.34e-75 234
Msa0174990 MtrunA17_Chr5g0432351 41.234 308 172 6 36 338 20 323 1.09e-74 234
Msa0174990 MtrunA17_Chr4g0072091 41.118 304 167 6 41 336 24 323 1.27e-73 231
Msa0174990 MtrunA17_Chr2g0320031 38.602 329 184 7 10 336 7 319 1.37e-73 231
Msa0174990 MtrunA17_Chr4g0013181 40.136 294 171 4 45 336 63 353 1.60e-73 231
Msa0174990 MtrunA17_Chr2g0320091 40.136 294 170 4 45 336 29 318 2.60e-73 230
Msa0174990 MtrunA17_Chr6g0460711 39.016 305 178 3 35 336 15 314 5.00e-73 229
Msa0174990 MtrunA17_Chr4g0013721 42.953 298 159 5 45 336 104 396 5.10e-73 232
Msa0174990 MtrunA17_Chr5g0438021 40.339 295 169 4 41 332 31 321 7.51e-73 229
Msa0174990 MtrunA17_Chr2g0292291 40.379 317 176 6 45 352 34 346 2.17e-72 229
Msa0174990 MtrunA17_Chr4g0063671 42.951 305 154 8 41 333 21 317 2.02e-71 225
Msa0174990 MtrunA17_Chr4g0068621 38.024 334 198 6 9 336 6 336 7.50e-71 224
Msa0174990 MtrunA17_Chr2g0296621 40.397 302 164 7 41 336 50 341 1.00e-70 224
Msa0174990 MtrunA17_Chr4g0075241 39.739 307 174 6 38 336 24 327 1.01e-70 224
Msa0174990 MtrunA17_Chr5g0432341 40.391 307 174 6 36 337 20 322 1.46e-70 223
Msa0174990 MtrunA17_Chr4g0013821 40.604 298 166 5 45 336 32 324 3.42e-70 223
Msa0174990 MtrunA17_Chr1g0148021 39.344 305 173 6 41 336 27 328 5.06e-70 222
Msa0174990 MtrunA17_Chr4g0046131 38.806 335 193 6 11 334 11 344 5.20e-70 222
Msa0174990 MtrunA17_Chr3g0129061 40.000 295 170 4 41 332 28 318 1.11e-69 221
Msa0174990 MtrunA17_Chr7g0224371 40.273 293 169 4 45 336 35 322 5.86e-69 219
Msa0174990 MtrunA17_Chr1g0197411 38.361 305 179 4 41 337 28 331 6.87e-69 219
Msa0174990 MtrunA17_Chr5g0432331 38.961 308 177 7 36 337 51 353 4.76e-68 218
Msa0174990 MtrunA17_Chr4g0013191 42.955 291 157 6 50 336 55 340 1.13e-66 214
Msa0174990 MtrunA17_Chr5g0421411 39.350 277 153 7 72 336 12 285 1.27e-66 212
Msa0174990 MtrunA17_Chr3g0129041 39.661 295 171 4 41 332 28 318 9.74e-66 211
Msa0174990 MtrunA17_Chr3g0106901 40.816 294 167 5 41 332 31 319 3.64e-65 209
Msa0174990 MtrunA17_Chr1g0181511 35.855 304 184 4 41 338 27 325 5.38e-65 209
Msa0174990 MtrunA17_Chr4g0076211 38.591 298 168 6 46 337 46 334 8.87e-65 208
Msa0174990 MtrunA17_Chr8g0364291 37.097 310 184 5 36 336 17 324 1.27e-64 208
Msa0174990 MtrunA17_Chr3g0107881 38.138 333 183 11 10 336 9 324 2.11e-64 207
Msa0174990 MtrunA17_Chr1g0213061 39.535 301 171 7 41 336 27 321 3.09e-63 204
Msa0174990 MtrunA17_Chr1g0181431 34.098 305 194 3 38 338 24 325 3.03e-62 202
Msa0174990 MtrunA17_Chr4g0074851 34.653 303 191 3 40 338 31 330 3.84e-62 202
Msa0174990 MtrunA17_Chr2g0329641 36.919 344 194 8 7 337 3 336 1.15e-61 201
Msa0174990 MtrunA17_Chr1g0181481 34.106 302 192 3 41 338 27 325 2.21e-60 197
Msa0174990 MtrunA17_Chr6g0451571 36.913 298 181 4 41 332 34 330 6.94e-59 193
Msa0174990 MtrunA17_Chr3g0136751 36.688 308 181 5 37 336 21 322 7.57e-58 190
Msa0174990 MtrunA17_Chr2g0278671 36.275 306 179 7 41 336 24 323 1.83e-57 189
Msa0174990 MtrunA17_Chr2g0279061 42.366 262 134 6 85 336 12 266 2.01e-57 187
Msa0174990 MtrunA17_Chr2g0316481 34.441 331 176 7 41 333 76 403 1.04e-56 190
Msa0174990 MtrunA17_Chr2g0316791 34.139 331 177 7 41 333 76 403 1.27e-56 189
Msa0174990 MtrunA17_Chr2g0316831 33.837 331 178 7 41 333 112 439 7.78e-55 186
Msa0174990 MtrunA17_Chr2g0311251 33.837 331 177 9 41 333 11 337 4.03e-54 181
Msa0174990 MtrunA17_Chr2g0316191 33.434 332 179 7 41 333 76 404 7.00e-54 182
Msa0174990 MtrunA17_Chr2g0311241 30.645 372 175 8 41 333 60 427 2.64e-51 176
Msa0174990 MtrunA17_Chr5g0413051 34.641 306 189 5 41 336 73 377 4.16e-51 174
Msa0174990 MtrunA17_Chr2g0292281 42.544 228 112 6 132 352 15 230 1.15e-48 164
Msa0174990 MtrunA17_Chr5g0432401 33.893 298 147 8 41 332 29 282 3.33e-47 161
Msa0174990 MtrunA17_Chr4g0004591 29.375 320 156 6 41 333 77 353 1.40e-38 140
Msa0174990 MtrunA17_Chr3g0114461 36.559 186 113 4 153 336 10 192 7.67e-36 129
Msa0174990 MtrunA17_Chr2g0316811 34.025 241 134 5 41 259 50 287 1.61e-35 132
Msa0174990 MtrunA17_Chr2g0316141 29.371 286 142 8 87 333 11 275 7.44e-29 113
Msa0174990 MtrunA17_Chr2g0316181 29.371 286 142 8 87 333 11 275 7.44e-29 113
Msa0174990 MtrunA17_Chr3g0114491 41.935 124 66 2 26 149 8 125 2.30e-26 102
Msa0174990 MtrunA17_Chr1g0181501 32.461 191 121 4 153 338 4 191 2.15e-25 101
Msa0174990 MtrunA17_Chr5g0432321 38.655 119 71 1 163 281 16 132 4.99e-21 87.8
Msa0174990 MtrunA17_Chr1g0181491 36.190 105 64 2 45 149 16 117 3.40e-18 80.5
Msa0174990 MtrunA17_Chr6g0468731 54.167 72 28 2 80 151 22 88 4.04e-18 78.6
Msa0174990 MtrunA17_Chr3g0104101 32.394 142 83 5 207 336 9 149 7.04e-18 79.7
Msa0174990 MtrunA17_Chr7g0268491 51.351 74 35 1 263 336 25 97 6.19e-16 72.8
Msa0174990 MtrunA17_Chr5g0423911 51.515 66 32 0 267 332 2 67 8.37e-16 72.8
Msa0174990 MtrunA17_Chr1g0196191 49.254 67 32 1 83 149 27 91 5.63e-15 71.6
Msa0174990 MtrunA17_Chr7g0254181 39.252 107 59 3 103 209 22 122 3.17e-14 69.7
Msa0174990 MtrunA17_Chr4g0013811 45.946 74 39 1 152 225 5 77 2.69e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0174990 AT1G71695.1 59.544 351 135 3 11 357 10 357 5.24e-160 452
Msa0174990 AT2G18150.1 43.729 295 157 5 45 332 40 332 1.49e-85 262
Msa0174990 AT4G36430.1 43.051 295 159 5 45 332 34 326 1.51e-84 259
Msa0174990 AT2G18140.1 44.068 295 156 5 45 332 39 331 1.73e-84 259
Msa0174990 AT5G14130.1 42.415 323 170 4 19 336 19 330 2.11e-83 256
Msa0174990 AT5G06730.1 40.741 351 186 8 1 341 1 339 6.42e-83 256
Msa0174990 AT5G14130.2 42.415 323 170 4 19 336 60 371 7.16e-83 256
Msa0174990 AT5G66390.1 44.068 295 156 5 45 332 37 329 2.83e-82 254
Msa0174990 AT1G05260.1 44.660 309 159 7 36 336 21 325 5.95e-82 253
Msa0174990 AT4G08780.1 43.168 322 169 7 41 352 24 341 7.35e-82 253
Msa0174990 AT3G21770.1 44.201 319 155 8 25 334 22 326 3.31e-81 251
Msa0174990 AT4G33420.1 41.693 319 167 5 14 332 22 321 4.41e-81 250
Msa0174990 AT4G33420.2 41.693 319 167 5 14 332 69 368 1.62e-80 251
Msa0174990 AT1G49570.1 43.279 305 162 4 37 332 44 346 1.91e-80 249
Msa0174990 AT3G32980.1 42.433 337 167 9 24 348 23 344 7.32e-80 248
Msa0174990 AT4G16270.1 45.246 305 152 8 41 336 50 348 8.60e-80 248
Msa0174990 AT4G08770.1 41.420 338 177 8 25 352 15 341 1.62e-79 247
Msa0174990 AT5G51890.1 44.013 309 158 7 36 336 21 322 3.78e-79 245
Msa0174990 AT1G44970.1 39.368 348 197 6 1 336 1 346 1.13e-78 245
Msa0174990 AT3G49120.1 41.411 326 170 7 24 339 24 338 1.64e-78 245
Msa0174990 AT4G11290.1 41.543 337 171 8 9 336 7 326 3.12e-78 243
Msa0174990 AT5G42180.1 41.196 301 164 5 38 332 20 313 3.66e-78 243
Msa0174990 AT3G50990.1 42.384 302 165 5 38 332 38 337 4.15e-78 243
Msa0174990 AT5G06720.1 40.667 300 168 5 41 332 32 329 7.12e-78 243
Msa0174990 AT5G05340.1 40.803 299 170 4 41 336 30 324 1.69e-76 239
Msa0174990 AT5G58400.1 38.602 329 182 5 7 332 10 321 2.36e-76 238
Msa0174990 AT2G18980.1 41.118 304 166 6 41 336 25 323 2.67e-76 238
Msa0174990 AT2G22420.1 42.140 299 162 5 45 337 27 320 3.03e-76 238
Msa0174990 AT5G19890.1 41.503 306 161 7 41 336 30 327 5.81e-76 238
Msa0174990 AT5G15180.1 41.234 308 171 5 35 336 26 329 5.99e-76 238
Msa0174990 AT1G68850.1 38.889 342 183 10 10 336 5 335 1.14e-75 237
Msa0174990 AT3G03670.1 40.000 310 173 5 35 336 17 321 1.89e-75 236
Msa0174990 AT5G64120.1 39.157 332 186 8 12 336 6 328 2.93e-75 236
Msa0174990 AT1G14550.1 39.634 328 182 6 11 336 8 321 4.17e-75 235
Msa0174990 AT2G38390.1 43.478 322 154 9 28 336 27 333 6.94e-75 235
Msa0174990 AT2G35380.1 42.722 316 157 9 36 336 29 335 1.03e-74 234
Msa0174990 AT4G26010.1 39.604 303 173 5 35 336 16 309 1.75e-74 233
Msa0174990 AT3G49110.1 41.100 309 170 6 41 339 33 339 1.92e-74 234
Msa0174990 AT1G05240.1 42.105 304 164 6 41 336 26 325 1.98e-74 233
Msa0174990 AT1G05250.1 42.105 304 164 6 41 336 26 325 1.98e-74 233
Msa0174990 AT4G26010.2 39.604 303 173 5 35 336 44 337 2.32e-74 234
Msa0174990 AT4G30170.1 39.145 304 172 5 41 336 27 325 2.32e-74 233
Msa0174990 AT3G01190.1 42.434 304 163 5 39 336 24 321 2.37e-74 233
Msa0174990 AT5G17820.1 41.751 297 157 5 41 332 24 309 2.72e-74 233
Msa0174990 AT5G17820.2 41.980 293 154 5 45 332 81 362 4.02e-73 231
Msa0174990 AT4G37530.1 39.803 304 174 4 41 336 27 329 6.25e-73 229
Msa0174990 AT2G38380.1 42.547 322 157 9 28 336 27 333 1.93e-72 229
Msa0174990 AT1G14540.1 41.611 298 169 5 41 336 21 315 7.78e-71 224
Msa0174990 AT5G40150.1 39.933 298 176 2 41 336 32 328 1.17e-70 224
Msa0174990 AT1G34510.1 38.944 303 175 5 35 336 16 309 1.27e-70 223
Msa0174990 AT4G37520.1 38.816 304 177 4 41 336 27 329 4.11e-70 223
Msa0174990 AT4G17690.1 39.216 306 180 3 34 337 22 323 6.73e-70 222
Msa0174990 AT5G58390.1 40.541 296 168 5 41 332 21 312 1.41e-69 221
Msa0174990 AT5G39580.1 40.461 304 168 6 39 336 23 319 1.44e-69 221
Msa0174990 AT2G34060.1 37.092 337 201 5 8 334 9 344 2.94e-69 221
Msa0174990 AT5G67400.1 39.344 305 174 6 41 336 27 329 2.23e-68 218
Msa0174990 AT2G41480.1 40.789 304 160 8 45 336 33 328 1.36e-67 216
Msa0174990 AT4G37520.2 38.158 304 176 5 41 336 27 326 1.88e-67 216
Msa0174990 AT3G49960.1 38.361 305 177 6 41 336 27 329 4.58e-67 214
Msa0174990 AT3G28200.1 39.667 300 173 5 41 336 21 316 1.70e-66 213
Msa0174990 AT5G19880.1 38.944 303 172 6 41 332 25 325 5.44e-66 212
Msa0174990 AT2G24800.1 40.584 308 168 6 38 336 28 329 1.33e-65 211
Msa0174990 AT4G33870.1 35.030 334 173 5 41 333 68 398 3.38e-65 212
Msa0174990 AT5G64110.1 40.397 302 161 7 45 336 38 330 5.95e-65 209
Msa0174990 AT4G21960.1 35.238 315 194 4 28 338 21 329 6.55e-65 209
Msa0174990 AT5G24070.1 37.719 342 190 8 10 338 6 337 8.79e-65 209
Msa0174990 AT2G39040.1 40.453 309 167 8 41 336 46 350 5.11e-62 202
Msa0174990 AT4G31760.1 36.364 330 187 6 15 336 12 326 1.30e-61 200
Msa0174990 AT5G64100.1 39.667 300 164 7 45 336 41 331 2.50e-61 200
Msa0174990 AT5G47000.1 36.949 295 182 3 41 332 33 326 6.54e-61 199
Msa0174990 AT1G77100.1 36.637 333 185 9 10 336 7 319 9.31e-61 198
Msa0174990 AT5G22410.1 39.604 303 163 9 41 332 28 321 2.08e-60 197
Msa0174990 AT4G37530.2 40.078 257 145 4 41 289 27 282 8.87e-60 194
Msa0174990 AT2G43480.1 35.882 340 189 9 13 332 1 331 1.01e-59 196
Msa0174990 AT3G17070.1 35.099 302 186 4 39 332 36 335 2.03e-59 195
Msa0174990 AT4G25980.1 36.808 307 177 6 41 336 26 326 2.32e-58 192
Msa0174990 AT1G24110.1 35.238 315 189 6 32 336 13 322 7.84e-58 191
Msa0174990 AT1G30870.1 36.508 315 181 8 36 342 46 349 2.91e-57 190
Msa0174990 AT2G37130.1 34.106 302 189 5 41 336 30 327 3.49e-57 189
Msa0174990 AT2G37130.2 34.228 298 186 5 45 336 3 296 6.12e-57 187
Msa0174990 AT2G35380.2 42.000 250 125 8 102 336 3 247 2.17e-54 179
Msa0174990 AT3G17070.2 32.558 258 163 5 83 332 18 272 2.92e-40 143
Msa0174990 AT5G39580.2 38.806 201 112 4 39 239 23 212 1.00e-38 138
Msa0174990 AT4G25980.2 40.588 170 95 3 41 210 71 234 3.82e-35 130
Msa0174990 AT2G24800.2 42.690 171 92 3 38 208 28 192 9.08e-33 122
Msa0174990 AT3G42570.1 29.907 107 73 1 102 208 43 147 6.14e-11 60.5

Find 78 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGCTGCTATGCTTAAAA+TGG 0.176723 1_4:-60766463 Msa0174990:CDS
TACTTGTAAGAATGTTAATT+TGG 0.190227 1_4:-60766194 Msa0174990:three_prime_UTR
AAGTAGGACAAGTTTGTTTA+AGG 0.199321 1_4:+60766670 None:intergenic
TCCATGACAGGGTCTTGTTT+TGG 0.232885 1_4:+60766711 None:intergenic
TTAGAAATGTGATTAATAAT+AGG 0.261648 1_4:-60766324 Msa0174990:exon
CTTCCATGACTGCTTTGTTC+AGG 0.336694 1_4:-60767474 Msa0174990:intron
ATTGTTGTTGTGTTGCTTGA+TGG 0.350284 1_4:+60766843 None:intergenic
TAAGGAACATTATTACATTC+TGG 0.363842 1_4:+60766266 None:intergenic
TTGTGATGGATCAGTATTAT+TGG 0.364319 1_4:-60767225 Msa0174990:CDS
CTTCACTATAGGAGGAGGTT+TGG 0.382309 1_4:+60767616 None:intergenic
CACCACCAAACTTGAGTCTT+AGG 0.384280 1_4:-60767169 Msa0174990:CDS
CAATGGCTTATGCTTCTTGT+AGG 0.384357 1_4:-60767712 Msa0174990:CDS
CTACTACATCTGTAGGGTTA+AGG 0.401776 1_4:+60766796 None:intergenic
CTGATGATAGATTCAAGCTT+AGG 0.402937 1_4:+60767560 None:intergenic
GTAAAACTAACCTCTTGAAA+AGG 0.405283 1_4:+60766288 None:intergenic
TTAATCCATCTCTCCTTCCT+AGG 0.406924 1_4:+60766907 None:intergenic
ATTAGCACGAATTTCTCCTT+TGG 0.416931 1_4:+60766416 None:intergenic
TTCGTGCTAATTGCTCTGTT+AGG 0.418468 1_4:-60766404 Msa0174990:CDS
GGTGGTGCATCTTTCTCACT+TGG 0.424168 1_4:+60767185 None:intergenic
CATGATAGTCCCTTCACTAT+AGG 0.430598 1_4:+60767605 None:intergenic
TTCGATTATCTTGAAGGCCT+CGG 0.436787 1_4:+60767145 None:intergenic
GTATTTGTTATCAAATTTGT+TGG 0.437290 1_4:+60766608 None:intergenic
TATTTGTTATCAAATTTGTT+GGG 0.439495 1_4:+60766609 None:intergenic
CCATTACTATAGTACAAGTA+AGG 0.443155 1_4:+60766248 None:intergenic
TCTTATCAGTGTATAAATCT+TGG 0.449979 1_4:+60766544 None:intergenic
AAAGGTATGGATTAGAAGAA+AGG 0.455618 1_4:+60767676 None:intergenic
CCTTACTTGTACTATAGTAA+TGG 0.460323 1_4:-60766248 Msa0174990:three_prime_UTR
CTCTCAGGTGGTCACACAAT+AGG 0.463629 1_4:-60766771 Msa0174990:CDS
AAGATTTATACACTGATAAG+AGG 0.464368 1_4:-60766542 Msa0174990:CDS
GATGATGTTGGTCAAGCTGC+TGG 0.480697 1_4:-60767512 Msa0174990:CDS
AACTGTGGTGTTATCAGTGT+TGG 0.488314 1_4:+60766647 None:intergenic
GTTGCTGCTATGCTTAAAAT+GGG 0.493223 1_4:-60766462 Msa0174990:CDS
TAAAACTAACCTCTTGAAAA+GGG 0.498597 1_4:+60766289 None:intergenic
TCGGTCCTAAGACTCAAGTT+TGG 0.499279 1_4:+60767164 None:intergenic
CAAATCTTTCCTTACTTCTG+TGG 0.502716 1_4:-60766370 Msa0174990:CDS
ATGAAAATGCAGGGTTGTGA+TGG 0.506653 1_4:-60767239 Msa0174990:intron
CATCTTCCACCACAGAAGTA+AGG 0.507687 1_4:+60766361 None:intergenic
GATGTGAGAAAATGAAAGAA+AGG 0.511303 1_4:+60767658 None:intergenic
ACAGATGTAGTAGCACTCTC+AGG 0.517970 1_4:-60766786 Msa0174990:CDS
GAGAAAATGAAAGAAAGGTA+TGG 0.521173 1_4:+60767663 None:intergenic
AACAGCATCACGAGCGGCAA+TGG 0.523212 1_4:+60767061 None:intergenic
ATCTTTCCTTACTTCTGTGG+TGG 0.523496 1_4:-60766367 Msa0174990:CDS
TGGATTAACATTTGCAACAC+AGG 0.523958 1_4:-60766892 Msa0174990:CDS
TGTTGATCTTATGAACCGTC+AGG 0.528938 1_4:-60766583 Msa0174990:CDS
CTATAGTGAAGGGACTATCA+TGG 0.530164 1_4:-60767604 Msa0174990:CDS
GTCCTAAGACTCAAGTTTGG+TGG 0.530334 1_4:+60767167 None:intergenic
TAATCCATCTCTCCTTCCTA+GGG 0.531504 1_4:+60766908 None:intergenic
AAGATCTTCAATGATGATGT+TGG 0.544356 1_4:-60767524 Msa0174990:CDS
CAAAGCAGTCATGGAAGTGA+AGG 0.552183 1_4:+60767480 None:intergenic
GAGAGTGCTACTACATCTGT+AGG 0.561499 1_4:+60766789 None:intergenic
CCTGTCATGGACAAAACCTA+TGG 0.564791 1_4:-60766699 Msa0174990:CDS
ATACACTGATAAGAGGACTA+AGG 0.565750 1_4:-60766535 Msa0174990:CDS
AGTCCCTTCACTATAGGAGG+AGG 0.565917 1_4:+60767611 None:intergenic
TTACCTGAACAAAGCAGTCA+TGG 0.566092 1_4:+60767471 None:intergenic
GTGTTATCAGTGTTGGAAGT+AGG 0.569777 1_4:+60766654 None:intergenic
GATAGTCCCTTCACTATAGG+AGG 0.573316 1_4:+60767608 None:intergenic
CCGTCAGGCTCTCTTTACTT+CGG 0.573471 1_4:-60766568 Msa0174990:CDS
TACTTCTGTGGTGGAAGATG+CGG 0.576534 1_4:-60766358 Msa0174990:CDS
CAAACCTCCTCCTATAGTGA+AGG 0.591712 1_4:-60767615 Msa0174990:CDS
ACTACAAAATTCCCCTAGGA+AGG 0.593729 1_4:-60766920 Msa0174990:CDS
ATTCCCCTAGGAAGGAGAGA+TGG 0.594591 1_4:-60766912 Msa0174990:CDS
GATGGATCAGTATTATTGGA+TGG 0.596952 1_4:-60767221 Msa0174990:CDS
TTCTGTGGTGGAAGATGCGG+TGG 0.600054 1_4:-60766355 Msa0174990:CDS
GTTGTGTTGCTTGATGGTGA+AGG 0.611201 1_4:+60766849 None:intergenic
AGAGTGCTACTACATCTGTA+GGG 0.620697 1_4:+60766790 None:intergenic
CTAATTTCTACATATCACAA+TGG 0.621170 1_4:-60767729 None:intergenic
CCCAAAACAAGACCCTGTCA+TGG 0.624304 1_4:-60766712 Msa0174990:CDS
AAACCTCCTCCTATAGTGAA+GGG 0.624659 1_4:-60767614 Msa0174990:CDS
CCAGACTACAAAATTCCCCT+AGG 0.644230 1_4:-60766924 Msa0174990:CDS
TGTTTGTGAAAGAACTGCAG+TGG 0.657795 1_4:+60766742 None:intergenic
ACTACGAATATCTAGAACTG+TGG 0.661587 1_4:+60766632 None:intergenic
ATTAACATTTGCAACACAGG+AGG 0.668426 1_4:-60766889 Msa0174990:CDS
CCGAAGTAAAGAGAGCCTGA+CGG 0.686185 1_4:+60766568 None:intergenic
GATGTAGTAGCACTCTCAGG+TGG 0.686682 1_4:-60766783 Msa0174990:CDS
AGTAATATAGAGAAGCAGTG+TGG 0.694261 1_4:-60767113 Msa0174990:CDS
TAGGAAAACAGCATCACGAG+CGG 0.695239 1_4:+60767055 None:intergenic
AATCCATCTCTCCTTCCTAG+GGG 0.697642 1_4:+60766909 None:intergenic
AAACTTGAGTCTTAGGACCG+AGG 0.714645 1_4:-60767162 Msa0174990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATATTTGGTTATTTA+TGG - chr1_4:60766616-60766635 Msa0174990:CDS 10.0%
!! TTATTATATTTGGTTATTTA+TGG - chr1_4:60766616-60766635 Msa0174990:CDS 10.0%
!! AACTATAAACAATACTAATT+AGG - chr1_4:60766480-60766499 Msa0174990:CDS 15.0%
!! AAATCCAAACTTATTATATT+TGG - chr1_4:60766606-60766625 Msa0174990:CDS 15.0%
!! ATAACCAAATATAATAAGTT+TGG + chr1_4:60766613-60766632 None:intergenic 15.0%
!!! TATTTGTTATCAAATTTGTT+GGG + chr1_4:60767280-60767299 None:intergenic 15.0%
!! TTAGAAATGTGATTAATAAT+AGG - chr1_4:60767562-60767581 Msa0174990:CDS 15.0%
!! AACTATAAACAATACTAATT+AGG - chr1_4:60766480-60766499 Msa0174990:CDS 15.0%
!! AAATCCAAACTTATTATATT+TGG - chr1_4:60766606-60766625 Msa0174990:CDS 15.0%
!! ATAACCAAATATAATAAGTT+TGG + chr1_4:60766613-60766632 None:intergenic 15.0%
!!! TATTTGTTATCAAATTTGTT+GGG + chr1_4:60767280-60767299 None:intergenic 15.0%
!! TTAGAAATGTGATTAATAAT+AGG - chr1_4:60767562-60767581 Msa0174990:CDS 15.0%
!! GTATATTTAGCTAATTAGTT+AGG - chr1_4:60766449-60766468 Msa0174990:CDS 20.0%
!! GTTAAAAAAATGAAAATGCA+GGG - chr1_4:60766638-60766657 Msa0174990:CDS 20.0%
!! AAAATAAAGGTTTACTTACT+AGG + chr1_4:60766853-60766872 None:intergenic 20.0%
!!! GTATTTGTTATCAAATTTGT+TGG + chr1_4:60767281-60767300 None:intergenic 20.0%
!! TACTTGTAAGAATGTTAATT+TGG - chr1_4:60767692-60767711 Msa0174990:CDS 20.0%
!! GTATATTTAGCTAATTAGTT+AGG - chr1_4:60766449-60766468 Msa0174990:CDS 20.0%
!! GTTAAAAAAATGAAAATGCA+GGG - chr1_4:60766638-60766657 Msa0174990:CDS 20.0%
!! AAAATAAAGGTTTACTTACT+AGG + chr1_4:60766853-60766872 None:intergenic 20.0%
!!! GTATTTGTTATCAAATTTGT+TGG + chr1_4:60767281-60767300 None:intergenic 20.0%
!! TACTTGTAAGAATGTTAATT+TGG - chr1_4:60767692-60767711 Msa0174990:CDS 20.0%
!!! CAATGTTTTATTGACATATG+TGG - chr1_4:60766546-60766565 Msa0174990:CDS 25.0%
! GGTTAAAAAAATGAAAATGC+AGG - chr1_4:60766637-60766656 Msa0174990:CDS 25.0%
!!! AAGTTTTTCGATTATCTTGA+AGG + chr1_4:60766750-60766769 None:intergenic 25.0%
! TCTTATCAGTGTATAAATCT+TGG + chr1_4:60767345-60767364 None:intergenic 25.0%
! AAGATTTATACACTGATAAG+AGG - chr1_4:60767344-60767363 Msa0174990:intron 25.0%
! TAAAACTAACCTCTTGAAAA+GGG + chr1_4:60767600-60767619 None:intergenic 25.0%
! TAAGGAACATTATTACATTC+TGG + chr1_4:60767623-60767642 None:intergenic 25.0%
!!! CAATGTTTTATTGACATATG+TGG - chr1_4:60766546-60766565 Msa0174990:CDS 25.0%
! GGTTAAAAAAATGAAAATGC+AGG - chr1_4:60766637-60766656 Msa0174990:CDS 25.0%
!!! AAGTTTTTCGATTATCTTGA+AGG + chr1_4:60766750-60766769 None:intergenic 25.0%
! TCTTATCAGTGTATAAATCT+TGG + chr1_4:60767345-60767364 None:intergenic 25.0%
! AAGATTTATACACTGATAAG+AGG - chr1_4:60767344-60767363 Msa0174990:intron 25.0%
! TAAAACTAACCTCTTGAAAA+GGG + chr1_4:60767600-60767619 None:intergenic 25.0%
! TAAGGAACATTATTACATTC+TGG + chr1_4:60767623-60767642 None:intergenic 25.0%
!! AAAGGTATGGATTAGAAGAA+AGG + chr1_4:60766213-60766232 None:intergenic 30.0%
GAGAAAATGAAAGAAAGGTA+TGG + chr1_4:60766226-60766245 None:intergenic 30.0%
GATGTGAGAAAATGAAAGAA+AGG + chr1_4:60766231-60766250 None:intergenic 30.0%
! AAGATCTTCAATGATGATGT+TGG - chr1_4:60766362-60766381 Msa0174990:CDS 30.0%
! ATAGAAGCTGTACATTTTCA+CGG + chr1_4:60766525-60766544 None:intergenic 30.0%
TGAATCTGTTTAGCTAACTT+TGG - chr1_4:60766583-60766602 Msa0174990:CDS 30.0%
!! TTGTGATGGATCAGTATTAT+TGG - chr1_4:60766661-60766680 Msa0174990:CDS 30.0%
CCAACTTCTCACAAAAATAA+AGG + chr1_4:60766866-60766885 None:intergenic 30.0%
!!! CCTTTATTTTTGTGAGAAGT+TGG - chr1_4:60766863-60766882 Msa0174990:CDS 30.0%
!! TTGTTTAAGGTTTTTGCCAT+AGG + chr1_4:60767206-60767225 None:intergenic 30.0%
! AAGTAGGACAAGTTTGTTTA+AGG + chr1_4:60767219-60767238 None:intergenic 30.0%
!! TATTTGTTTCCCTTTTCAAG+AGG - chr1_4:60767588-60767607 Msa0174990:CDS 30.0%
GTAAAACTAACCTCTTGAAA+AGG + chr1_4:60767601-60767620 None:intergenic 30.0%
CCATTACTATAGTACAAGTA+AGG + chr1_4:60767641-60767660 None:intergenic 30.0%
CCTTACTTGTACTATAGTAA+TGG - chr1_4:60767638-60767657 Msa0174990:CDS 30.0%
!! AAAGGTATGGATTAGAAGAA+AGG + chr1_4:60766213-60766232 None:intergenic 30.0%
GAGAAAATGAAAGAAAGGTA+TGG + chr1_4:60766226-60766245 None:intergenic 30.0%
GATGTGAGAAAATGAAAGAA+AGG + chr1_4:60766231-60766250 None:intergenic 30.0%
! AAGATCTTCAATGATGATGT+TGG - chr1_4:60766362-60766381 Msa0174990:CDS 30.0%
! ATAGAAGCTGTACATTTTCA+CGG + chr1_4:60766525-60766544 None:intergenic 30.0%
TGAATCTGTTTAGCTAACTT+TGG - chr1_4:60766583-60766602 Msa0174990:CDS 30.0%
!! TTGTGATGGATCAGTATTAT+TGG - chr1_4:60766661-60766680 Msa0174990:CDS 30.0%
CCAACTTCTCACAAAAATAA+AGG + chr1_4:60766866-60766885 None:intergenic 30.0%
!!! CCTTTATTTTTGTGAGAAGT+TGG - chr1_4:60766863-60766882 Msa0174990:CDS 30.0%
!! TTGTTTAAGGTTTTTGCCAT+AGG + chr1_4:60767206-60767225 None:intergenic 30.0%
! AAGTAGGACAAGTTTGTTTA+AGG + chr1_4:60767219-60767238 None:intergenic 30.0%
!! TATTTGTTTCCCTTTTCAAG+AGG - chr1_4:60767588-60767607 Msa0174990:CDS 30.0%
GTAAAACTAACCTCTTGAAA+AGG + chr1_4:60767601-60767620 None:intergenic 30.0%
CCATTACTATAGTACAAGTA+AGG + chr1_4:60767641-60767660 None:intergenic 30.0%
CCTTACTTGTACTATAGTAA+TGG - chr1_4:60767638-60767657 Msa0174990:CDS 30.0%
! AGGACATTTTGAATCGTAGA+AGG + chr1_4:60766309-60766328 None:intergenic 35.0%
CTGATGATAGATTCAAGCTT+AGG + chr1_4:60766329-60766348 None:intergenic 35.0%
GATGGATCAGTATTATTGGA+TGG - chr1_4:60766665-60766684 Msa0174990:CDS 35.0%
AGTAATATAGAGAAGCAGTG+TGG - chr1_4:60766773-60766792 Msa0174990:CDS 35.0%
!! CATGTTTTTACATTGCAGTC+AGG - chr1_4:60766935-60766954 Msa0174990:CDS 35.0%
TGGATTAACATTTGCAACAC+AGG - chr1_4:60766994-60767013 Msa0174990:intron 35.0%
ATTAACATTTGCAACACAGG+AGG - chr1_4:60766997-60767016 Msa0174990:intron 35.0%
!! ATTGTTGTTGTGTTGCTTGA+TGG + chr1_4:60767046-60767065 None:intergenic 35.0%
!!! TGTAGGGTTAAGGTTTTTTG+TGG + chr1_4:60767083-60767102 None:intergenic 35.0%
! ACTACGAATATCTAGAACTG+TGG + chr1_4:60767257-60767276 None:intergenic 35.0%
ATACACTGATAAGAGGACTA+AGG - chr1_4:60767351-60767370 Msa0174990:intron 35.0%
TGTTGCTGCTATGCTTAAAA+TGG - chr1_4:60767423-60767442 Msa0174990:intron 35.0%
GTTGCTGCTATGCTTAAAAT+GGG - chr1_4:60767424-60767443 Msa0174990:intron 35.0%
!!! AATGTTTTGACTGGAACCAA+AGG - chr1_4:60767454-60767473 Msa0174990:intron 35.0%
ATTAGCACGAATTTCTCCTT+TGG + chr1_4:60767473-60767492 None:intergenic 35.0%
CAAATCTTTCCTTACTTCTG+TGG - chr1_4:60767516-60767535 Msa0174990:CDS 35.0%
! AGGACATTTTGAATCGTAGA+AGG + chr1_4:60766309-60766328 None:intergenic 35.0%
CTGATGATAGATTCAAGCTT+AGG + chr1_4:60766329-60766348 None:intergenic 35.0%
GATGGATCAGTATTATTGGA+TGG - chr1_4:60766665-60766684 Msa0174990:CDS 35.0%
AGTAATATAGAGAAGCAGTG+TGG - chr1_4:60766773-60766792 Msa0174990:CDS 35.0%
!! CATGTTTTTACATTGCAGTC+AGG - chr1_4:60766935-60766954 Msa0174990:CDS 35.0%
TGGATTAACATTTGCAACAC+AGG - chr1_4:60766994-60767013 Msa0174990:intron 35.0%
ATTAACATTTGCAACACAGG+AGG - chr1_4:60766997-60767016 Msa0174990:intron 35.0%
!! ATTGTTGTTGTGTTGCTTGA+TGG + chr1_4:60767046-60767065 None:intergenic 35.0%
!!! TGTAGGGTTAAGGTTTTTTG+TGG + chr1_4:60767083-60767102 None:intergenic 35.0%
! ACTACGAATATCTAGAACTG+TGG + chr1_4:60767257-60767276 None:intergenic 35.0%
ATACACTGATAAGAGGACTA+AGG - chr1_4:60767351-60767370 Msa0174990:intron 35.0%
TGTTGCTGCTATGCTTAAAA+TGG - chr1_4:60767423-60767442 Msa0174990:intron 35.0%
GTTGCTGCTATGCTTAAAAT+GGG - chr1_4:60767424-60767443 Msa0174990:intron 35.0%
!!! AATGTTTTGACTGGAACCAA+AGG - chr1_4:60767454-60767473 Msa0174990:intron 35.0%
ATTAGCACGAATTTCTCCTT+TGG + chr1_4:60767473-60767492 None:intergenic 35.0%
CAAATCTTTCCTTACTTCTG+TGG - chr1_4:60767516-60767535 Msa0174990:CDS 35.0%
CAATGGCTTATGCTTCTTGT+AGG - chr1_4:60766174-60766193 Msa0174990:three_prime_UTR 40.0%
AAACCTCCTCCTATAGTGAA+GGG - chr1_4:60766272-60766291 Msa0174990:three_prime_UTR 40.0%
CATGATAGTCCCTTCACTAT+AGG + chr1_4:60766284-60766303 None:intergenic 40.0%
CTATAGTGAAGGGACTATCA+TGG - chr1_4:60766282-60766301 Msa0174990:three_prime_UTR 40.0%
TTACCTGAACAAAGCAGTCA+TGG + chr1_4:60766418-60766437 None:intergenic 40.0%
ATGAAAATGCAGGGTTGTGA+TGG - chr1_4:60766647-60766666 Msa0174990:CDS 40.0%
TTCGATTATCTTGAAGGCCT+CGG + chr1_4:60766744-60766763 None:intergenic 40.0%
! GTTTTTACATTGCAGTCAGG+AGG - chr1_4:60766938-60766957 Msa0174990:intron 40.0%
ACTACAAAATTCCCCTAGGA+AGG - chr1_4:60766966-60766985 Msa0174990:intron 40.0%
TAATCCATCTCTCCTTCCTA+GGG + chr1_4:60766981-60767000 None:intergenic 40.0%
TTAATCCATCTCTCCTTCCT+AGG + chr1_4:60766982-60767001 None:intergenic 40.0%
CTACTACATCTGTAGGGTTA+AGG + chr1_4:60767093-60767112 None:intergenic 40.0%
! AGAGTGCTACTACATCTGTA+GGG + chr1_4:60767099-60767118 None:intergenic 40.0%
TGTTTGTGAAAGAACTGCAG+TGG + chr1_4:60767147-60767166 None:intergenic 40.0%
!! CATAGGTTTTGTCCATGACA+GGG + chr1_4:60767189-60767208 None:intergenic 40.0%
! GTGTTATCAGTGTTGGAAGT+AGG + chr1_4:60767235-60767254 None:intergenic 40.0%
!! AACTGTGGTGTTATCAGTGT+TGG + chr1_4:60767242-60767261 None:intergenic 40.0%
TGTTGATCTTATGAACCGTC+AGG - chr1_4:60767303-60767322 Msa0174990:intron 40.0%
!!! GGACAGCTTAATGTTTTGAC+TGG - chr1_4:60767445-60767464 Msa0174990:intron 40.0%
TTCGTGCTAATTGCTCTGTT+AGG - chr1_4:60767482-60767501 Msa0174990:CDS 40.0%
ATCTTTCCTTACTTCTGTGG+TGG - chr1_4:60767519-60767538 Msa0174990:CDS 40.0%
CAATGGCTTATGCTTCTTGT+AGG - chr1_4:60766174-60766193 Msa0174990:three_prime_UTR 40.0%
AAACCTCCTCCTATAGTGAA+GGG - chr1_4:60766272-60766291 Msa0174990:three_prime_UTR 40.0%
CATGATAGTCCCTTCACTAT+AGG + chr1_4:60766284-60766303 None:intergenic 40.0%
CTATAGTGAAGGGACTATCA+TGG - chr1_4:60766282-60766301 Msa0174990:three_prime_UTR 40.0%
TTACCTGAACAAAGCAGTCA+TGG + chr1_4:60766418-60766437 None:intergenic 40.0%
ATGAAAATGCAGGGTTGTGA+TGG - chr1_4:60766647-60766666 Msa0174990:CDS 40.0%
TTCGATTATCTTGAAGGCCT+CGG + chr1_4:60766744-60766763 None:intergenic 40.0%
! GTTTTTACATTGCAGTCAGG+AGG - chr1_4:60766938-60766957 Msa0174990:intron 40.0%
ACTACAAAATTCCCCTAGGA+AGG - chr1_4:60766966-60766985 Msa0174990:intron 40.0%
TAATCCATCTCTCCTTCCTA+GGG + chr1_4:60766981-60767000 None:intergenic 40.0%
TTAATCCATCTCTCCTTCCT+AGG + chr1_4:60766982-60767001 None:intergenic 40.0%
CTACTACATCTGTAGGGTTA+AGG + chr1_4:60767093-60767112 None:intergenic 40.0%
! AGAGTGCTACTACATCTGTA+GGG + chr1_4:60767099-60767118 None:intergenic 40.0%
TGTTTGTGAAAGAACTGCAG+TGG + chr1_4:60767147-60767166 None:intergenic 40.0%
!! CATAGGTTTTGTCCATGACA+GGG + chr1_4:60767189-60767208 None:intergenic 40.0%
! GTGTTATCAGTGTTGGAAGT+AGG + chr1_4:60767235-60767254 None:intergenic 40.0%
!! AACTGTGGTGTTATCAGTGT+TGG + chr1_4:60767242-60767261 None:intergenic 40.0%
TGTTGATCTTATGAACCGTC+AGG - chr1_4:60767303-60767322 Msa0174990:intron 40.0%
!!! GGACAGCTTAATGTTTTGAC+TGG - chr1_4:60767445-60767464 Msa0174990:intron 40.0%
TTCGTGCTAATTGCTCTGTT+AGG - chr1_4:60767482-60767501 Msa0174990:CDS 40.0%
ATCTTTCCTTACTTCTGTGG+TGG - chr1_4:60767519-60767538 Msa0174990:CDS 40.0%
CTTCACTATAGGAGGAGGTT+TGG + chr1_4:60766273-60766292 None:intergenic 45.0%
CAAACCTCCTCCTATAGTGA+AGG - chr1_4:60766271-60766290 Msa0174990:three_prime_UTR 45.0%
GATAGTCCCTTCACTATAGG+AGG + chr1_4:60766281-60766300 None:intergenic 45.0%
CAAAGCAGTCATGGAAGTGA+AGG + chr1_4:60766409-60766428 None:intergenic 45.0%
! CTTCCATGACTGCTTTGTTC+AGG - chr1_4:60766412-60766431 Msa0174990:CDS 45.0%
CACCACCAAACTTGAGTCTT+AGG - chr1_4:60766717-60766736 Msa0174990:CDS 45.0%
GTCCTAAGACTCAAGTTTGG+TGG + chr1_4:60766722-60766741 None:intergenic 45.0%
TCGGTCCTAAGACTCAAGTT+TGG + chr1_4:60766725-60766744 None:intergenic 45.0%
AAACTTGAGTCTTAGGACCG+AGG - chr1_4:60766724-60766743 Msa0174990:CDS 45.0%
TAGGAAAACAGCATCACGAG+CGG + chr1_4:60766834-60766853 None:intergenic 45.0%
! CCTAGGGGAATTTTGTAGTC+TGG + chr1_4:60766965-60766984 None:intergenic 45.0%
CCAGACTACAAAATTCCCCT+AGG - chr1_4:60766962-60766981 Msa0174990:intron 45.0%
AATCCATCTCTCCTTCCTAG+GGG + chr1_4:60766980-60766999 None:intergenic 45.0%
!! GTTGTGTTGCTTGATGGTGA+AGG + chr1_4:60767040-60767059 None:intergenic 45.0%
!! GAGAGTGCTACTACATCTGT+AGG + chr1_4:60767100-60767119 None:intergenic 45.0%
ACAGATGTAGTAGCACTCTC+AGG - chr1_4:60767100-60767119 Msa0174990:CDS 45.0%
!!! CCATGACAGGGTCTTGTTTT+GGG + chr1_4:60767177-60767196 None:intergenic 45.0%
!!! TCCATGACAGGGTCTTGTTT+TGG + chr1_4:60767178-60767197 None:intergenic 45.0%
!! CCATAGGTTTTGTCCATGAC+AGG + chr1_4:60767190-60767209 None:intergenic 45.0%
CCTGTCATGGACAAAACCTA+TGG - chr1_4:60767187-60767206 Msa0174990:CDS 45.0%
CATCTTCCACCACAGAAGTA+AGG + chr1_4:60767528-60767547 None:intergenic 45.0%
! TACTTCTGTGGTGGAAGATG+CGG - chr1_4:60767528-60767547 Msa0174990:CDS 45.0%
CTTCACTATAGGAGGAGGTT+TGG + chr1_4:60766273-60766292 None:intergenic 45.0%
CAAACCTCCTCCTATAGTGA+AGG - chr1_4:60766271-60766290 Msa0174990:three_prime_UTR 45.0%
GATAGTCCCTTCACTATAGG+AGG + chr1_4:60766281-60766300 None:intergenic 45.0%
CAAAGCAGTCATGGAAGTGA+AGG + chr1_4:60766409-60766428 None:intergenic 45.0%
! CTTCCATGACTGCTTTGTTC+AGG - chr1_4:60766412-60766431 Msa0174990:CDS 45.0%
CACCACCAAACTTGAGTCTT+AGG - chr1_4:60766717-60766736 Msa0174990:CDS 45.0%
GTCCTAAGACTCAAGTTTGG+TGG + chr1_4:60766722-60766741 None:intergenic 45.0%
TCGGTCCTAAGACTCAAGTT+TGG + chr1_4:60766725-60766744 None:intergenic 45.0%
AAACTTGAGTCTTAGGACCG+AGG - chr1_4:60766724-60766743 Msa0174990:CDS 45.0%
TAGGAAAACAGCATCACGAG+CGG + chr1_4:60766834-60766853 None:intergenic 45.0%
! CCTAGGGGAATTTTGTAGTC+TGG + chr1_4:60766965-60766984 None:intergenic 45.0%
CCAGACTACAAAATTCCCCT+AGG - chr1_4:60766962-60766981 Msa0174990:intron 45.0%
AATCCATCTCTCCTTCCTAG+GGG + chr1_4:60766980-60766999 None:intergenic 45.0%
!! GTTGTGTTGCTTGATGGTGA+AGG + chr1_4:60767040-60767059 None:intergenic 45.0%
!! GAGAGTGCTACTACATCTGT+AGG + chr1_4:60767100-60767119 None:intergenic 45.0%
ACAGATGTAGTAGCACTCTC+AGG - chr1_4:60767100-60767119 Msa0174990:CDS 45.0%
!!! CCATGACAGGGTCTTGTTTT+GGG + chr1_4:60767177-60767196 None:intergenic 45.0%
!!! TCCATGACAGGGTCTTGTTT+TGG + chr1_4:60767178-60767197 None:intergenic 45.0%
!! CCATAGGTTTTGTCCATGAC+AGG + chr1_4:60767190-60767209 None:intergenic 45.0%
CCTGTCATGGACAAAACCTA+TGG - chr1_4:60767187-60767206 Msa0174990:CDS 45.0%
CATCTTCCACCACAGAAGTA+AGG + chr1_4:60767528-60767547 None:intergenic 45.0%
! TACTTCTGTGGTGGAAGATG+CGG - chr1_4:60767528-60767547 Msa0174990:CDS 45.0%
AGTCCCTTCACTATAGGAGG+AGG + chr1_4:60766278-60766297 None:intergenic 50.0%
GATGATGTTGGTCAAGCTGC+TGG - chr1_4:60766374-60766393 Msa0174990:CDS 50.0%
GGTGGTGCATCTTTCTCACT+TGG + chr1_4:60766704-60766723 None:intergenic 50.0%
ATTCCCCTAGGAAGGAGAGA+TGG - chr1_4:60766974-60766993 Msa0174990:intron 50.0%
GATGTAGTAGCACTCTCAGG+TGG - chr1_4:60767103-60767122 Msa0174990:CDS 50.0%
CTCTCAGGTGGTCACACAAT+AGG - chr1_4:60767115-60767134 Msa0174990:CDS 50.0%
CCCAAAACAAGACCCTGTCA+TGG - chr1_4:60767174-60767193 Msa0174990:CDS 50.0%
CCGAAGTAAAGAGAGCCTGA+CGG + chr1_4:60767321-60767340 None:intergenic 50.0%
CCGTCAGGCTCTCTTTACTT+CGG - chr1_4:60767318-60767337 Msa0174990:intron 50.0%
AGTCCCTTCACTATAGGAGG+AGG + chr1_4:60766278-60766297 None:intergenic 50.0%
GATGATGTTGGTCAAGCTGC+TGG - chr1_4:60766374-60766393 Msa0174990:CDS 50.0%
GGTGGTGCATCTTTCTCACT+TGG + chr1_4:60766704-60766723 None:intergenic 50.0%
ATTCCCCTAGGAAGGAGAGA+TGG - chr1_4:60766974-60766993 Msa0174990:intron 50.0%
GATGTAGTAGCACTCTCAGG+TGG - chr1_4:60767103-60767122 Msa0174990:CDS 50.0%
CTCTCAGGTGGTCACACAAT+AGG - chr1_4:60767115-60767134 Msa0174990:CDS 50.0%
CCCAAAACAAGACCCTGTCA+TGG - chr1_4:60767174-60767193 Msa0174990:CDS 50.0%
CCGAAGTAAAGAGAGCCTGA+CGG + chr1_4:60767321-60767340 None:intergenic 50.0%
CCGTCAGGCTCTCTTTACTT+CGG - chr1_4:60767318-60767337 Msa0174990:intron 50.0%
AACAGCATCACGAGCGGCAA+TGG + chr1_4:60766828-60766847 None:intergenic 55.0%
! TTCTGTGGTGGAAGATGCGG+TGG - chr1_4:60767531-60767550 Msa0174990:CDS 55.0%
AACAGCATCACGAGCGGCAA+TGG + chr1_4:60766828-60766847 None:intergenic 55.0%
! TTCTGTGGTGGAAGATGCGG+TGG - chr1_4:60767531-60767550 Msa0174990:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 60766170 60767738 60766170 ID=Msa0174990;Name=Msa0174990
chr1_4 mRNA 60766170 60767738 60766170 ID=Msa0174990-mRNA-1;Parent=Msa0174990;Name=Msa0174990-mRNA-1;_AED=0.06;_eAED=0.06;_QI=6|1|1|1|1|1|3|165|357
chr1_4 exon 60766170 60766955 60766170 ID=Msa0174990-mRNA-1:exon:19274;Parent=Msa0174990-mRNA-1
chr1_4 exon 60767055 60767249 60767055 ID=Msa0174990-mRNA-1:exon:19273;Parent=Msa0174990-mRNA-1
chr1_4 exon 60767475 60767738 60767475 ID=Msa0174990-mRNA-1:exon:19272;Parent=Msa0174990-mRNA-1
chr1_4 five_prime_UTR 60767733 60767738 60767733 ID=Msa0174990-mRNA-1:five_prime_utr;Parent=Msa0174990-mRNA-1
chr1_4 CDS 60767475 60767732 60767475 ID=Msa0174990-mRNA-1:cds;Parent=Msa0174990-mRNA-1
chr1_4 CDS 60767055 60767249 60767055 ID=Msa0174990-mRNA-1:cds;Parent=Msa0174990-mRNA-1
chr1_4 CDS 60766335 60766955 60766335 ID=Msa0174990-mRNA-1:cds;Parent=Msa0174990-mRNA-1
chr1_4 three_prime_UTR 60766170 60766334 60766170 ID=Msa0174990-mRNA-1:three_prime_utr;Parent=Msa0174990-mRNA-1
Gene Sequence

>Msa0174990

ATGGCTTATGCTTCTTGTAGGAGTGCTTTCTTCACCTTTCTTCTAATCCATACCTTTCTTTCATTTTCTCACATCAAAGTGTCTGAAGCACAAGCCAAACCTCCTCCTATAGTGAAGGGACTATCATGGACCTTCTACGATTCAAAATGTCCTAAGCTTGAATCTATCATCAGAAAAGAGCTCAAGAAGATCTTCAATGATGATGTTGGTCAAGCTGCTGGCTTGCTTCGCCTTCACTTCCATGACTGCTTTGTTCAGGGTTGTGATGGATCAGTATTATTGGATGGATCAGCAAGTGAGCCAAGTGAGAAAGATGCACCACCAAACTTGAGTCTTAGGACCGAGGCCTTCAAGATAATCGAAAAACTTCGTAGTAATATAGAGAAGCAGTGTGGAAGAATCGTCTCTTGTGCTGATATCACTGCCATTGCCGCTCGTGATGCTGTTTTCCTATCAGGAGGACCAGACTACAAAATTCCCCTAGGAAGGAGAGATGGATTAACATTTGCAACACAGGAGGTAACATTAGACAATCTTCCTTCACCATCAAGCAACACAACAACAATCTTAAACTCACTTGCCACAAAAAACCTTAACCCTACAGATGTAGTAGCACTCTCAGGTGGTCACACAATAGGCATAAGCCACTGCAGTTCTTTCACAAACAGACTCTACCCAAAACAAGACCCTGTCATGGACAAAACCTATGGCAAAAACCTTAAACAAACTTGTCCTACTTCCAACACTGATAACACCACAGTTCTAGATATTCGTAGTCCCAACAAATTTGATAACAAATACTATGTTGATCTTATGAACCGTCAGGCTCTCTTTACTTCGGACCAAGATTTATACACTGATAAGAGGACTAAGGATATTGTTACTAACTTTGCTGTGAATCAATCTTTGTTCTTTGAGAAATTTGTTGCTGCTATGCTTAAAATGGGACAGCTTAATGTTTTGACTGGAACCAAAGGAGAAATTCGTGCTAATTGCTCTGTTAGGAATAAAAATAGCAAATCTTTCCTTACTTCTGTGGTGGAAGATGCGGTGGAAAGATTTTTAGAAATGTGA
Protein sequence

>Msa0174990

MAYASCRSAFFTFLLIHTFLSFSHIKVSEAQAKPPPIVKGLSWTFYDSKCPKLESIIRKELKKIFNDDVGQAAGLLRLHFHDCFVQGCDGSVLLDGSASEPSEKDAPPNLSLRTEAFKIIEKLRSNIEKQCGRIVSCADITAIAARDAVFLSGGPDYKIPLGRRDGLTFATQEVTLDNLPSPSSNTTTILNSLATKNLNPTDVVALSGGHTIGISHCSSFTNRLYPKQDPVMDKTYGKNLKQTCPTSNTDNTTVLDIRSPNKFDNKYYVDLMNRQALFTSDQDLYTDKRTKDIVTNFAVNQSLFFEKFVAAMLKMGQLNVLTGTKGEIRANCSVRNKNSKSFLTSVVEDAVERFLEM*