AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0088520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0088520 MtrunA17_Chr1g0205701 97.297 185 5 0 1 185 1 185 3.02e-132 368
Msa0088520 MtrunA17_Chr5g0394321 70.909 165 41 3 1 164 1 159 6.22e-85 248
Msa0088520 MtrunA17_Chr4g0064041 39.264 163 89 3 1 157 1 159 3.29e-27 105
Msa0088520 MtrunA17_Chr4g0064041 46.000 100 52 1 25 122 191 290 2.08e-26 103
Msa0088520 MtrunA17_Chr8g0335531 33.540 161 88 6 12 162 11 162 4.87e-27 101
Msa0088520 MtrunA17_Chr2g0323021 37.013 154 81 6 8 148 6 156 2.92e-24 95.1
Msa0088520 MtrunA17_Chr7g0253761 36.571 175 104 4 1 173 1 170 1.10e-23 92.0
Msa0088520 MtrunA17_Chr1g0177341 38.017 121 71 3 6 124 5 123 7.66e-23 89.4
Msa0088520 MtrunA17_Chr1g0177331 38.017 121 71 3 6 124 5 123 7.66e-23 89.4
Msa0088520 MtrunA17_Chr7g0253681 38.095 126 73 4 1 123 1 124 1.30e-22 89.0
Msa0088520 MtrunA17_Chr3g0132431 36.364 143 84 5 5 145 4 141 2.31e-22 89.0
Msa0088520 MtrunA17_Chr7g0253701 36.800 125 75 3 1 123 1 123 2.65e-22 88.2
Msa0088520 MtrunA17_Chr7g0253671 36.800 125 75 3 1 123 1 123 2.65e-22 88.2
Msa0088520 MtrunA17_Chr3g0132441 41.748 103 57 3 20 122 21 120 4.64e-22 88.2
Msa0088520 MtrunA17_Chr4g0040291 30.380 158 106 1 22 175 22 179 6.25e-22 87.8
Msa0088520 MtrunA17_Chr8g0335571 36.842 114 67 4 12 123 11 121 1.16e-21 87.4
Msa0088520 MtrunA17_Chr2g0331181 33.566 143 85 4 19 158 21 156 1.42e-21 87.0
Msa0088520 MtrunA17_Chr2g0301431 37.168 113 66 2 9 121 16 123 2.77e-21 84.3
Msa0088520 MtrunA17_Chr3g0125761 38.393 112 64 2 10 121 18 124 7.29e-21 83.6
Msa0088520 MtrunA17_Chr1g0196801 34.091 132 80 4 22 151 20 146 1.29e-20 84.3
Msa0088520 MtrunA17_Chr6g0483981 34.524 168 98 6 7 166 7 170 2.11e-20 87.8
Msa0088520 MtrunA17_Chr7g0253641 41.045 134 70 4 7 140 14 138 2.18e-20 84.7
Msa0088520 MtrunA17_Chr7g0253731 35.043 117 72 3 1 116 1 114 3.00e-20 82.8
Msa0088520 MtrunA17_Chr3g0132511 36.667 120 71 4 6 122 5 122 1.02e-19 82.8
Msa0088520 MtrunA17_Chr1g0188981 35.976 164 99 4 25 183 29 191 1.28e-19 82.0
Msa0088520 MtrunA17_Chr8g0380901 32.456 114 72 2 8 121 11 119 1.51e-19 79.7
Msa0088520 MtrunA17_Chr3g0132701 36.667 120 71 4 6 122 5 122 1.68e-19 82.0
Msa0088520 MtrunA17_Chr1g0150131 35.922 103 64 1 43 145 46 146 2.38e-19 80.9
Msa0088520 MtrunA17_Chr3g0136871 31.655 139 80 3 43 178 48 174 4.98e-19 80.1
Msa0088520 MtrunA17_Chr1g0211101 32.800 125 77 3 7 126 11 133 5.37e-19 80.1
Msa0088520 MtrunA17_Chr6g0459441 35.294 102 62 3 22 122 23 121 8.27e-19 79.3
Msa0088520 MtrunA17_Chr3g0125791 39.394 99 55 3 22 120 30 123 8.77e-19 78.2
Msa0088520 MtrunA17_Chr3g0132411 34.677 124 76 4 1 122 39 159 2.66e-18 77.8
Msa0088520 MtrunA17_Chr8g0380711 35.514 107 59 4 18 121 21 120 3.80e-18 76.3
Msa0088520 MtrunA17_Chr8g0360371 29.104 134 92 2 4 134 1 134 7.12e-18 79.3
Msa0088520 MtrunA17_Chr2g0324721 30.168 179 108 4 1 162 1 179 9.76e-18 77.0
Msa0088520 MtrunA17_Chr4g0039721 35.338 133 76 4 25 149 32 162 1.48e-17 76.3
Msa0088520 MtrunA17_Chr4g0062341 33.129 163 89 7 25 183 32 178 3.20e-17 75.5
Msa0088520 MtrunA17_Chr4g0062311 33.129 163 89 7 25 183 32 178 3.20e-17 75.5
Msa0088520 MtrunA17_Chr7g0253631 38.636 132 71 4 7 138 14 135 7.40e-17 75.5
Msa0088520 MtrunA17_Chr4g0041811 32.743 113 74 1 13 123 20 132 1.04e-16 74.7
Msa0088520 MtrunA17_Chr6g0458331 37.255 102 59 2 25 123 37 136 1.29e-16 73.6
Msa0088520 MtrunA17_Chr6g0481491 33.673 98 62 2 25 122 28 122 1.33e-16 74.3
Msa0088520 MtrunA17_Chr8g0387804 30.709 127 76 3 6 122 3 127 1.34e-15 69.7
Msa0088520 MtrunA17_Chr4g0064031 34.615 130 77 4 15 139 18 144 1.78e-15 70.5
Msa0088520 MtrunA17_Chr1g0185541 45.455 66 36 0 57 122 5 70 2.82e-15 67.4
Msa0088520 MtrunA17_Chr6g0453921 30.303 132 83 4 13 142 26 150 2.87e-15 70.1
Msa0088520 MtrunA17_Chr5g0440804 35.000 120 72 4 28 145 33 148 5.18e-15 69.3
Msa0088520 MtrunA17_Chr2g0306461 29.310 116 80 2 8 122 11 125 1.41e-14 68.9
Msa0088520 MtrunA17_Chr6g0453801 30.769 117 76 3 8 122 18 131 3.81e-14 67.0
Msa0088520 MtrunA17_Chr8g0385021 28.947 114 80 1 8 120 7 120 5.70e-14 68.2
Msa0088520 MtrunA17_Chr6g0453791 30.233 129 82 5 24 150 30 152 8.19e-14 66.2
Msa0088520 MtrunA17_Chr2g0289741 31.624 117 76 3 8 122 9 123 2.02e-13 65.1
Msa0088520 MtrunA17_Chr2g0324711 29.139 151 106 1 8 157 8 158 2.66e-13 67.0
Msa0088520 MtrunA17_Chr4g0033131 28.369 141 90 4 1 134 1 137 8.51e-13 63.5
Msa0088520 MtrunA17_Chr4g0033531 28.369 141 90 4 1 134 1 137 8.51e-13 63.5
Msa0088520 MtrunA17_Chr8g0385031 30.233 172 105 4 8 166 7 176 1.51e-12 64.3
Msa0088520 MtrunA17_Chr3g0144921 27.333 150 92 6 4 146 3 142 3.82e-12 61.6
Msa0088520 MtrunA17_Chr5g0440711 29.861 144 92 5 4 142 6 145 4.40e-12 60.8
Msa0088520 MtrunA17_Chr2g0317231 29.245 106 75 0 20 125 20 125 5.06e-12 63.5
Msa0088520 MtrunA17_Chr2g0319611 25.600 125 89 2 3 123 4 128 1.46e-11 61.2
Msa0088520 MtrunA17_Chr4g0001571 31.915 94 61 2 27 120 31 121 5.65e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0088520 AT5G26330.1 59.394 165 66 1 10 174 9 172 1.48e-66 202
Msa0088520 AT2G31050.1 42.636 129 72 2 10 137 13 140 1.68e-33 118
Msa0088520 AT2G26720.1 37.589 141 83 2 1 137 1 140 1.87e-33 118
Msa0088520 AT2G32300.1 34.940 166 93 5 1 165 1 152 3.09e-31 114
Msa0088520 AT2G32300.2 34.940 166 93 5 1 165 31 182 1.11e-30 113
Msa0088520 AT3G17675.1 42.708 96 53 1 27 122 34 127 1.72e-23 90.5
Msa0088520 AT3G27200.1 32.432 111 71 3 14 123 15 122 4.54e-23 90.9
Msa0088520 AT4G31840.1 33.333 156 92 4 28 180 31 177 1.07e-22 90.1
Msa0088520 AT5G07475.1 35.570 149 91 3 14 162 19 162 2.11e-22 89.7
Msa0088520 AT4G28365.1 29.944 177 108 4 1 161 1 177 6.60e-21 85.9
Msa0088520 AT3G53330.1 36.190 105 66 1 19 122 182 286 7.02e-21 87.8
Msa0088520 AT3G53330.1 40.230 87 46 3 7 92 8 89 2.52e-14 70.1
Msa0088520 AT3G60270.1 37.864 103 61 2 21 123 22 121 4.05e-20 83.6
Msa0088520 AT1G45063.2 40.000 100 59 1 24 122 148 247 1.89e-19 83.6
Msa0088520 AT1G45063.2 32.812 128 80 3 1 123 1 127 1.50e-18 81.3
Msa0088520 AT1G45063.1 40.000 100 59 1 24 122 148 247 2.37e-19 84.3
Msa0088520 AT1G45063.1 32.812 128 80 3 1 123 1 127 1.96e-18 82.0
Msa0088520 AT2G02850.1 31.858 113 72 2 8 120 19 126 4.84e-19 79.0
Msa0088520 AT5G20230.1 34.646 127 78 2 25 150 25 147 5.22e-19 80.9
Msa0088520 AT5G53870.2 29.545 176 110 4 1 165 1 173 1.49e-18 82.0
Msa0088520 AT2G25060.1 33.588 131 81 3 10 134 14 144 1.69e-18 79.0
Msa0088520 AT1G72230.1 35.433 127 80 2 40 165 36 161 1.86e-18 79.0
Msa0088520 AT5G53870.1 29.545 176 110 4 1 165 1 173 2.59e-18 81.6
Msa0088520 AT3G60280.1 39.806 103 59 2 21 123 20 119 2.89e-18 79.3
Msa0088520 AT3G20570.1 31.034 116 78 2 9 123 15 129 4.65e-18 78.6
Msa0088520 AT1G64640.1 29.341 167 92 4 4 161 6 155 4.92e-18 78.2
Msa0088520 AT4G30590.1 31.538 130 81 4 1 123 1 129 2.15e-17 76.3
Msa0088520 AT1G79800.1 31.618 136 84 3 9 137 18 151 4.65e-17 75.5
Msa0088520 AT5G57920.1 32.632 95 60 1 43 133 44 138 8.92e-17 74.7
Msa0088520 AT5G15350.1 33.835 133 80 4 7 137 10 136 9.96e-17 74.3
Msa0088520 AT4G27520.1 31.034 145 93 3 9 146 15 159 1.15e-16 76.6
Msa0088520 AT5G57920.2 27.007 137 94 2 3 133 1 137 1.61e-16 73.9
Msa0088520 AT5G57920.3 31.250 112 71 2 28 133 59 170 3.95e-16 73.6
Msa0088520 AT1G22480.1 31.746 189 105 7 1 183 1 171 2.55e-15 70.5
Msa0088520 AT2G44790.1 37.634 93 53 3 33 123 36 125 2.65e-15 71.2
Msa0088520 AT3G18590.1 34.286 105 59 5 25 123 27 127 5.78e-15 70.1
Msa0088520 AT5G25090.1 33.333 81 54 0 43 123 47 127 6.44e-15 69.7
Msa0088520 AT4G32490.1 27.811 169 99 4 8 158 12 175 3.80e-14 68.2
Msa0088520 AT2G23990.1 35.000 80 51 1 44 122 49 128 5.14e-14 67.8
Msa0088520 AT1G48940.1 31.538 130 77 6 5 126 4 129 1.69e-13 65.9
Msa0088520 AT5G14345.1 36.275 102 59 3 41 141 35 131 2.03e-13 64.7
Msa0088520 AT4G12880.1 33.010 103 64 3 22 122 26 125 3.72e-13 63.9
Msa0088520 AT4G01380.1 28.205 156 79 6 30 180 77 204 4.46e-13 65.1
Msa0088520 AT2G27035.1 25.161 155 104 5 2 155 8 151 4.76e-12 61.6
Msa0088520 AT1G17800.1 29.167 120 78 4 8 123 21 137 1.37e-11 59.7
Msa0088520 AT3G01070.1 32.653 98 61 3 9 104 12 106 5.42e-11 58.9

Find 39 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATGATTTGTTATCTTTA+TGG 0.100250 1_2:-69604258 None:intergenic
AAATGTGATCATATTATTAT+AGG 0.226490 1_2:-69604704 None:intergenic
TTGTAATTTGTTTACTAGTT+TGG 0.295248 1_2:+69604562 Msa0088520:three_prime_UTR
TGTTTACTAGTTTGGTTTGT+GGG 0.329596 1_2:+69604570 Msa0088520:three_prime_UTR
TCTGTAATTCCTTTACTTGC+TGG 0.332337 1_2:+69604625 Msa0088520:three_prime_UTR
ATCATCTTCACCTTCAGCAT+TGG 0.417122 1_2:+69604379 Msa0088520:CDS
TTGTTTACTAGTTTGGTTTG+TGG 0.424475 1_2:+69604569 Msa0088520:three_prime_UTR
TCATATTATTATAGGACAAC+TGG 0.437115 1_2:-69604696 None:intergenic
TTCAGTGGTGCAGCATTACT+AGG 0.451042 1_2:-69604452 None:intergenic
GTCACCAAGATGGAAGTTCT+TGG 0.461769 1_2:-69603411 None:intergenic
AGAGTCTATAGTCATGGCTT+TGG 0.467395 1_2:+69603259 Msa0088520:exon
CCAATTGCAACATTCACTAC+TGG 0.482441 1_2:+69604227 Msa0088520:CDS
TTCATATGCAGCTGTATACA+AGG 0.487437 1_2:+69603328 Msa0088520:CDS
CCAGTAGTGAATGTTGCAAT+TGG 0.487702 1_2:-69604227 None:intergenic
AGGTTGGTGATTCTGCTGGC+TGG 0.497516 1_2:+69603348 Msa0088520:CDS
TACAAGGTTGGTGATTCTGC+TGG 0.511253 1_2:+69603344 Msa0088520:CDS
ACCATAAAGATAACAAATCA+TGG 0.515142 1_2:+69604257 Msa0088520:CDS
TGTCCTGCTTGACAGTGTCC+AGG 0.520727 1_2:-69604305 None:intergenic
GGCTTTGGTAGAAAGAGCTA+TGG 0.525531 1_2:+69603274 Msa0088520:CDS
AGGAGAAGGTGCAGGTACAT+TGG 0.525671 1_2:-69604432 None:intergenic
GCTGGAACTGTAGCTTCAGC+AGG 0.528803 1_2:-69604407 None:intergenic
GCTACCAAGAACTTCCATCT+TGG 0.537592 1_2:+69603407 Msa0088520:CDS
TACTAATAGTGTCACCAAGA+TGG 0.545203 1_2:-69603421 None:intergenic
TAATTCTATCCAGCAAGTAA+AGG 0.554771 1_2:-69604634 None:intergenic
GCAGGAGAAGCCAATGCTGA+AGG 0.557674 1_2:-69604389 None:intergenic
GTACCTGGACACTGTCAAGC+AGG 0.560336 1_2:+69604302 Msa0088520:CDS
TATGCAGCTGTATACAAGGT+TGG 0.562762 1_2:+69603332 Msa0088520:CDS
GGTGCAGGTACATTGGAAGC+TGG 0.574590 1_2:-69604425 None:intergenic
AATGCTGAAGGTGAAGATGA+TGG 0.589576 1_2:-69604377 None:intergenic
TTTAAAGCAATACATTTCAG+TGG 0.593570 1_2:-69604467 None:intergenic
ATTAACAGCAAGAAATTGCA+TGG 0.600755 1_2:-69604510 None:intergenic
TCTTGCAGAGTCTATAGTCA+TGG 0.602471 1_2:+69603253 Msa0088520:exon
ACACTGTCAAGCAGGACAGA+AGG 0.613934 1_2:+69604310 Msa0088520:CDS
GGTGCAGCATTACTAGGAGA+AGG 0.618539 1_2:-69604446 None:intergenic
TCTGCTGGCTGGACTACACT+TGG 0.619967 1_2:+69603359 Msa0088520:CDS
GAAGGTGAAGATGATGGAGC+AGG 0.628782 1_2:-69604371 None:intergenic
TGCATTGCATGACTTGTACA+TGG 0.629046 1_2:-69604198 None:intergenic
GTGTCACTCGCATCACATTG+TGG 0.630592 1_2:-69604172 None:intergenic
GCATTACTAGGAGAAGGTGC+AGG 0.642038 1_2:-69604440 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTAAAATAATGTTAATTT+TGG - chr1_2:69603566-69603585 None:intergenic 10.0%
!!! TTGTAAAATAATGTTAATTT+TGG - chr1_2:69603566-69603585 None:intergenic 10.0%
!! ATTTACTAATACAAAAAACA+AGG + chr1_2:69603221-69603240 Msa0088520:five_prime_UTR 15.0%
!!! AACATTATTTTACAATTCTA+TGG + chr1_2:69603571-69603590 Msa0088520:intron 15.0%
!! TATTATATATATCTTTGTCT+TGG + chr1_2:69603779-69603798 Msa0088520:intron 15.0%
!! AAATGTGATCATATTATTAT+AGG - chr1_2:69604707-69604726 None:intergenic 15.0%
!! ATTTACTAATACAAAAAACA+AGG + chr1_2:69603221-69603240 Msa0088520:five_prime_UTR 15.0%
!!! AACATTATTTTACAATTCTA+TGG + chr1_2:69603571-69603590 Msa0088520:intron 15.0%
!! TATTATATATATCTTTGTCT+TGG + chr1_2:69603779-69603798 Msa0088520:intron 15.0%
!! AAATGTGATCATATTATTAT+AGG - chr1_2:69604707-69604726 None:intergenic 15.0%
!!! TAACATTGACTACAAAAAAT+GGG + chr1_2:69603382-69603401 Msa0088520:CDS 20.0%
!! TTAAAGCAAAAAACATGAAA+AGG - chr1_2:69603647-69603666 None:intergenic 20.0%
!!! CAAATTACATAATTAGTCTT+GGG - chr1_2:69603716-69603735 None:intergenic 20.0%
!! TATTTCTTCAAGAAATTAGA+CGG - chr1_2:69603805-69603824 None:intergenic 20.0%
!!! TTGTAATTTGTTTACTAGTT+TGG + chr1_2:69604562-69604581 Msa0088520:three_prime_UTR 20.0%
!!! TAACATTGACTACAAAAAAT+GGG + chr1_2:69603382-69603401 Msa0088520:CDS 20.0%
!! TTAAAGCAAAAAACATGAAA+AGG - chr1_2:69603647-69603666 None:intergenic 20.0%
!!! CAAATTACATAATTAGTCTT+GGG - chr1_2:69603716-69603735 None:intergenic 20.0%
!! TATTTCTTCAAGAAATTAGA+CGG - chr1_2:69603805-69603824 None:intergenic 20.0%
!!! TTGTAATTTGTTTACTAGTT+TGG + chr1_2:69604562-69604581 Msa0088520:three_prime_UTR 20.0%
!!! AGTAAATTTCTTTTTTGCTG+AGG - chr1_2:69603208-69603227 None:intergenic 25.0%
! TAATACAAAAAACAAGGAGA+AGG + chr1_2:69603227-69603246 Msa0088520:five_prime_UTR 25.0%
!!! TATGGTTTTGTCTATAATGA+TGG + chr1_2:69603292-69603311 Msa0088520:CDS 25.0%
!! GTAACATTGACTACAAAAAA+TGG + chr1_2:69603381-69603400 Msa0088520:CDS 25.0%
! GAACAAATTAAACATGCAAT+TGG - chr1_2:69603457-69603476 None:intergenic 25.0%
!! TTTTCTAACATTGTCCAATT+TGG + chr1_2:69603600-69603619 Msa0088520:intron 25.0%
!! TGAGTTTTTCTATACCAAAT+TGG - chr1_2:69603617-69603636 None:intergenic 25.0%
!! GCAAATTACATAATTAGTCT+TGG - chr1_2:69603717-69603736 None:intergenic 25.0%
! ATGAAAACAAAAAGTTGTGT+AGG - chr1_2:69603743-69603762 None:intergenic 25.0%
! TGATAAAAAATGTTCTAGTC+TGG + chr1_2:69603866-69603885 Msa0088520:intron 25.0%
!!! AACTCAATTTTGTTTTTGAG+AGG + chr1_2:69604077-69604096 Msa0088520:intron 25.0%
! ACCATAAAGATAACAAATCA+TGG + chr1_2:69604257-69604276 Msa0088520:CDS 25.0%
!!! ACCATGATTTGTTATCTTTA+TGG - chr1_2:69604261-69604280 None:intergenic 25.0%
!! TTTAAAGCAATACATTTCAG+TGG - chr1_2:69604470-69604489 None:intergenic 25.0%
!!! TATTGCTTTAAAAATGATGC+TGG + chr1_2:69604478-69604497 Msa0088520:CDS 25.0%
! AGAAGAAGTAAAAACCAAAA+AGG - chr1_2:69604596-69604615 None:intergenic 25.0%
!!! TTGGTTTTTACTTCTTCTAT+TGG + chr1_2:69604598-69604617 Msa0088520:three_prime_UTR 25.0%
! TCATATTATTATAGGACAAC+TGG - chr1_2:69604699-69604718 None:intergenic 25.0%
!!! AGTAAATTTCTTTTTTGCTG+AGG - chr1_2:69603208-69603227 None:intergenic 25.0%
! TAATACAAAAAACAAGGAGA+AGG + chr1_2:69603227-69603246 Msa0088520:five_prime_UTR 25.0%
!!! TATGGTTTTGTCTATAATGA+TGG + chr1_2:69603292-69603311 Msa0088520:CDS 25.0%
!! GTAACATTGACTACAAAAAA+TGG + chr1_2:69603381-69603400 Msa0088520:CDS 25.0%
! GAACAAATTAAACATGCAAT+TGG - chr1_2:69603457-69603476 None:intergenic 25.0%
!! TTTTCTAACATTGTCCAATT+TGG + chr1_2:69603600-69603619 Msa0088520:intron 25.0%
!! TGAGTTTTTCTATACCAAAT+TGG - chr1_2:69603617-69603636 None:intergenic 25.0%
!! GCAAATTACATAATTAGTCT+TGG - chr1_2:69603717-69603736 None:intergenic 25.0%
! ATGAAAACAAAAAGTTGTGT+AGG - chr1_2:69603743-69603762 None:intergenic 25.0%
! TGATAAAAAATGTTCTAGTC+TGG + chr1_2:69603866-69603885 Msa0088520:intron 25.0%
!!! AACTCAATTTTGTTTTTGAG+AGG + chr1_2:69604077-69604096 Msa0088520:intron 25.0%
! ACCATAAAGATAACAAATCA+TGG + chr1_2:69604257-69604276 Msa0088520:CDS 25.0%
!!! ACCATGATTTGTTATCTTTA+TGG - chr1_2:69604261-69604280 None:intergenic 25.0%
!! TTTAAAGCAATACATTTCAG+TGG - chr1_2:69604470-69604489 None:intergenic 25.0%
!!! TATTGCTTTAAAAATGATGC+TGG + chr1_2:69604478-69604497 Msa0088520:CDS 25.0%
! AGAAGAAGTAAAAACCAAAA+AGG - chr1_2:69604596-69604615 None:intergenic 25.0%
!!! TTGGTTTTTACTTCTTCTAT+TGG + chr1_2:69604598-69604617 Msa0088520:three_prime_UTR 25.0%
! TCATATTATTATAGGACAAC+TGG - chr1_2:69604699-69604718 None:intergenic 25.0%
TTAAACATGCAATTGGAGAA+AGG - chr1_2:69603450-69603469 None:intergenic 30.0%
!!! CACATTTTCTGAAGTTTGAA+AGG + chr1_2:69603519-69603538 Msa0088520:intron 30.0%
ATTGGACAATGTTAGAAAAG+AGG - chr1_2:69603599-69603618 None:intergenic 30.0%
AAGCAAAAAACATGAAAAGG+AGG - chr1_2:69603644-69603663 None:intergenic 30.0%
CATGAATATGAATCCGAATT+CGG - chr1_2:69603894-69603913 None:intergenic 30.0%
!!! TAAAAATGATGCTGGCTTTT+TGG + chr1_2:69604486-69604505 Msa0088520:CDS 30.0%
!!! AAAAATGATGCTGGCTTTTT+GGG + chr1_2:69604487-69604506 Msa0088520:CDS 30.0%
ATTAACAGCAAGAAATTGCA+TGG - chr1_2:69604513-69604532 None:intergenic 30.0%
!! TTGTTTACTAGTTTGGTTTG+TGG + chr1_2:69604569-69604588 Msa0088520:three_prime_UTR 30.0%
!! TGTTTACTAGTTTGGTTTGT+GGG + chr1_2:69604570-69604589 Msa0088520:three_prime_UTR 30.0%
TAATTCTATCCAGCAAGTAA+AGG - chr1_2:69604637-69604656 None:intergenic 30.0%
TTAAACATGCAATTGGAGAA+AGG - chr1_2:69603450-69603469 None:intergenic 30.0%
!!! CACATTTTCTGAAGTTTGAA+AGG + chr1_2:69603519-69603538 Msa0088520:intron 30.0%
ATTGGACAATGTTAGAAAAG+AGG - chr1_2:69603599-69603618 None:intergenic 30.0%
AAGCAAAAAACATGAAAAGG+AGG - chr1_2:69603644-69603663 None:intergenic 30.0%
CATGAATATGAATCCGAATT+CGG - chr1_2:69603894-69603913 None:intergenic 30.0%
!!! TAAAAATGATGCTGGCTTTT+TGG + chr1_2:69604486-69604505 Msa0088520:CDS 30.0%
!!! AAAAATGATGCTGGCTTTTT+GGG + chr1_2:69604487-69604506 Msa0088520:CDS 30.0%
ATTAACAGCAAGAAATTGCA+TGG - chr1_2:69604513-69604532 None:intergenic 30.0%
!! TTGTTTACTAGTTTGGTTTG+TGG + chr1_2:69604569-69604588 Msa0088520:three_prime_UTR 30.0%
!! TGTTTACTAGTTTGGTTTGT+GGG + chr1_2:69604570-69604589 Msa0088520:three_prime_UTR 30.0%
TAATTCTATCCAGCAAGTAA+AGG - chr1_2:69604637-69604656 None:intergenic 30.0%
!!! TTTCTTTTTTGCTGAGGTTG+AGG - chr1_2:69603202-69603221 None:intergenic 35.0%
TTCATATGCAGCTGTATACA+AGG + chr1_2:69603328-69603347 Msa0088520:CDS 35.0%
TACTAATAGTGTCACCAAGA+TGG - chr1_2:69603424-69603443 None:intergenic 35.0%
AAGTTTGAAAGGTCCTTTCA+AGG + chr1_2:69603530-69603549 Msa0088520:intron 35.0%
AGTTTGAAAGGTCCTTTCAA+GGG + chr1_2:69603531-69603550 Msa0088520:intron 35.0%
AAAGGTAATGCAAGTGAAAG+TGG - chr1_2:69603947-69603966 None:intergenic 35.0%
! TGCATTACCTTTTTGTTCTG+TGG + chr1_2:69603955-69603974 Msa0088520:intron 35.0%
! AACCATGCTCTTGATGATTT+GGG - chr1_2:69604031-69604050 None:intergenic 35.0%
! AAACCATGCTCTTGATGATT+TGG - chr1_2:69604032-69604051 None:intergenic 35.0%
TCTGTAATTCCTTTACTTGC+TGG + chr1_2:69604625-69604644 Msa0088520:three_prime_UTR 35.0%
!!! TTTCTTTTTTGCTGAGGTTG+AGG - chr1_2:69603202-69603221 None:intergenic 35.0%
TTCATATGCAGCTGTATACA+AGG + chr1_2:69603328-69603347 Msa0088520:CDS 35.0%
TACTAATAGTGTCACCAAGA+TGG - chr1_2:69603424-69603443 None:intergenic 35.0%
AAGTTTGAAAGGTCCTTTCA+AGG + chr1_2:69603530-69603549 Msa0088520:intron 35.0%
AGTTTGAAAGGTCCTTTCAA+GGG + chr1_2:69603531-69603550 Msa0088520:intron 35.0%
AAAGGTAATGCAAGTGAAAG+TGG - chr1_2:69603947-69603966 None:intergenic 35.0%
! TGCATTACCTTTTTGTTCTG+TGG + chr1_2:69603955-69603974 Msa0088520:intron 35.0%
! AACCATGCTCTTGATGATTT+GGG - chr1_2:69604031-69604050 None:intergenic 35.0%
! AAACCATGCTCTTGATGATT+TGG - chr1_2:69604032-69604051 None:intergenic 35.0%
TCTGTAATTCCTTTACTTGC+TGG + chr1_2:69604625-69604644 Msa0088520:three_prime_UTR 35.0%
TCTTGCAGAGTCTATAGTCA+TGG + chr1_2:69603253-69603272 Msa0088520:exon 40.0%
AGAGTCTATAGTCATGGCTT+TGG + chr1_2:69603259-69603278 Msa0088520:exon 40.0%
TATGCAGCTGTATACAAGGT+TGG + chr1_2:69603332-69603351 Msa0088520:CDS 40.0%
CAAGAAAGATAGTGCAACAG+TGG - chr1_2:69603677-69603696 None:intergenic 40.0%
CACTGTTGCACTATCTTTCT+TGG + chr1_2:69603675-69603694 Msa0088520:intron 40.0%
TTGCACTATCTTTCTTGGCA+TGG + chr1_2:69603680-69603699 Msa0088520:intron 40.0%
TAGTCTCGAGTTCGATTGAA+AGG + chr1_2:69603832-69603851 Msa0088520:intron 40.0%
TTCTAGTCTGGATCCGAATT+CGG + chr1_2:69603878-69603897 Msa0088520:intron 40.0%
GTAATGCAAGTGAAAGTGGT+GGG - chr1_2:69603943-69603962 None:intergenic 40.0%
! CAAAGCACCACAGAACAAAA+AGG - chr1_2:69603965-69603984 None:intergenic 40.0%
! TGCATTGCATGACTTGTACA+TGG - chr1_2:69604201-69604220 None:intergenic 40.0%
CCAGTAGTGAATGTTGCAAT+TGG - chr1_2:69604230-69604249 None:intergenic 40.0%
CCAATTGCAACATTCACTAC+TGG + chr1_2:69604227-69604246 Msa0088520:CDS 40.0%
! GGTCATCACTTCTTCTTTTG+TGG + chr1_2:69604278-69604297 Msa0088520:CDS 40.0%
! TTCTTCTTTTGTGGTGTACC+TGG + chr1_2:69604287-69604306 Msa0088520:CDS 40.0%
AATGCTGAAGGTGAAGATGA+TGG - chr1_2:69604380-69604399 None:intergenic 40.0%
ATCATCTTCACCTTCAGCAT+TGG + chr1_2:69604379-69604398 Msa0088520:CDS 40.0%
TCTTGCAGAGTCTATAGTCA+TGG + chr1_2:69603253-69603272 Msa0088520:exon 40.0%
AGAGTCTATAGTCATGGCTT+TGG + chr1_2:69603259-69603278 Msa0088520:exon 40.0%
TATGCAGCTGTATACAAGGT+TGG + chr1_2:69603332-69603351 Msa0088520:CDS 40.0%
CAAGAAAGATAGTGCAACAG+TGG - chr1_2:69603677-69603696 None:intergenic 40.0%
CACTGTTGCACTATCTTTCT+TGG + chr1_2:69603675-69603694 Msa0088520:intron 40.0%
TTGCACTATCTTTCTTGGCA+TGG + chr1_2:69603680-69603699 Msa0088520:intron 40.0%
TAGTCTCGAGTTCGATTGAA+AGG + chr1_2:69603832-69603851 Msa0088520:intron 40.0%
TTCTAGTCTGGATCCGAATT+CGG + chr1_2:69603878-69603897 Msa0088520:intron 40.0%
GTAATGCAAGTGAAAGTGGT+GGG - chr1_2:69603943-69603962 None:intergenic 40.0%
! CAAAGCACCACAGAACAAAA+AGG - chr1_2:69603965-69603984 None:intergenic 40.0%
! TGCATTGCATGACTTGTACA+TGG - chr1_2:69604201-69604220 None:intergenic 40.0%
CCAGTAGTGAATGTTGCAAT+TGG - chr1_2:69604230-69604249 None:intergenic 40.0%
CCAATTGCAACATTCACTAC+TGG + chr1_2:69604227-69604246 Msa0088520:CDS 40.0%
! GGTCATCACTTCTTCTTTTG+TGG + chr1_2:69604278-69604297 Msa0088520:CDS 40.0%
! TTCTTCTTTTGTGGTGTACC+TGG + chr1_2:69604287-69604306 Msa0088520:CDS 40.0%
AATGCTGAAGGTGAAGATGA+TGG - chr1_2:69604380-69604399 None:intergenic 40.0%
ATCATCTTCACCTTCAGCAT+TGG + chr1_2:69604379-69604398 Msa0088520:CDS 40.0%
! GGCTTTGGTAGAAAGAGCTA+TGG + chr1_2:69603274-69603293 Msa0088520:CDS 45.0%
!! TACAAGGTTGGTGATTCTGC+TGG + chr1_2:69603344-69603363 Msa0088520:CDS 45.0%
GCTACCAAGAACTTCCATCT+TGG + chr1_2:69603407-69603426 Msa0088520:CDS 45.0%
GTCACCAAGATGGAAGTTCT+TGG - chr1_2:69603414-69603433 None:intergenic 45.0%
TGGTTGTCTTCTCCCTTGAA+AGG - chr1_2:69603546-69603565 None:intergenic 45.0%
GGTAATGCAAGTGAAAGTGG+TGG - chr1_2:69603944-69603963 None:intergenic 45.0%
CATGCAATGACTTTGTCGTG+TGG + chr1_2:69604004-69604023 Msa0088520:intron 45.0%
GTCCCAAATCATCAAGAGCA+TGG + chr1_2:69604026-69604045 Msa0088520:intron 45.0%
TTCAGTGGTGCAGCATTACT+AGG - chr1_2:69604455-69604474 None:intergenic 45.0%
!!! GTTTGGTTTGTGGGCCTTTT+TGG + chr1_2:69604579-69604598 Msa0088520:three_prime_UTR 45.0%
! GGCTTTGGTAGAAAGAGCTA+TGG + chr1_2:69603274-69603293 Msa0088520:CDS 45.0%
!! TACAAGGTTGGTGATTCTGC+TGG + chr1_2:69603344-69603363 Msa0088520:CDS 45.0%
GCTACCAAGAACTTCCATCT+TGG + chr1_2:69603407-69603426 Msa0088520:CDS 45.0%
GTCACCAAGATGGAAGTTCT+TGG - chr1_2:69603414-69603433 None:intergenic 45.0%
TGGTTGTCTTCTCCCTTGAA+AGG - chr1_2:69603546-69603565 None:intergenic 45.0%
GGTAATGCAAGTGAAAGTGG+TGG - chr1_2:69603944-69603963 None:intergenic 45.0%
CATGCAATGACTTTGTCGTG+TGG + chr1_2:69604004-69604023 Msa0088520:intron 45.0%
GTCCCAAATCATCAAGAGCA+TGG + chr1_2:69604026-69604045 Msa0088520:intron 45.0%
TTCAGTGGTGCAGCATTACT+AGG - chr1_2:69604455-69604474 None:intergenic 45.0%
!!! GTTTGGTTTGTGGGCCTTTT+TGG + chr1_2:69604579-69604598 Msa0088520:three_prime_UTR 45.0%
! GTGTCACTCGCATCACATTG+TGG - chr1_2:69604175-69604194 None:intergenic 50.0%
ACACTGTCAAGCAGGACAGA+AGG + chr1_2:69604310-69604329 Msa0088520:CDS 50.0%
!! GAAGGTGAAGATGATGGAGC+AGG - chr1_2:69604374-69604393 None:intergenic 50.0%
! AGGAGAAGGTGCAGGTACAT+TGG - chr1_2:69604435-69604454 None:intergenic 50.0%
! GCATTACTAGGAGAAGGTGC+AGG - chr1_2:69604443-69604462 None:intergenic 50.0%
GGTGCAGCATTACTAGGAGA+AGG - chr1_2:69604449-69604468 None:intergenic 50.0%
! GTGTCACTCGCATCACATTG+TGG - chr1_2:69604175-69604194 None:intergenic 50.0%
ACACTGTCAAGCAGGACAGA+AGG + chr1_2:69604310-69604329 Msa0088520:CDS 50.0%
!! GAAGGTGAAGATGATGGAGC+AGG - chr1_2:69604374-69604393 None:intergenic 50.0%
! AGGAGAAGGTGCAGGTACAT+TGG - chr1_2:69604435-69604454 None:intergenic 50.0%
! GCATTACTAGGAGAAGGTGC+AGG - chr1_2:69604443-69604462 None:intergenic 50.0%
GGTGCAGCATTACTAGGAGA+AGG - chr1_2:69604449-69604468 None:intergenic 50.0%
!! AGGTTGGTGATTCTGCTGGC+TGG + chr1_2:69603348-69603367 Msa0088520:CDS 55.0%
! TCTGCTGGCTGGACTACACT+TGG + chr1_2:69603359-69603378 Msa0088520:CDS 55.0%
GTACCTGGACACTGTCAAGC+AGG + chr1_2:69604302-69604321 Msa0088520:CDS 55.0%
TGTCCTGCTTGACAGTGTCC+AGG - chr1_2:69604308-69604327 None:intergenic 55.0%
GCAGGAGAAGCCAATGCTGA+AGG - chr1_2:69604392-69604411 None:intergenic 55.0%
GCTGGAACTGTAGCTTCAGC+AGG - chr1_2:69604410-69604429 None:intergenic 55.0%
GGTGCAGGTACATTGGAAGC+TGG - chr1_2:69604428-69604447 None:intergenic 55.0%
!! AGGTTGGTGATTCTGCTGGC+TGG + chr1_2:69603348-69603367 Msa0088520:CDS 55.0%
! TCTGCTGGCTGGACTACACT+TGG + chr1_2:69603359-69603378 Msa0088520:CDS 55.0%
GTACCTGGACACTGTCAAGC+AGG + chr1_2:69604302-69604321 Msa0088520:CDS 55.0%
TGTCCTGCTTGACAGTGTCC+AGG - chr1_2:69604308-69604327 None:intergenic 55.0%
GCAGGAGAAGCCAATGCTGA+AGG - chr1_2:69604392-69604411 None:intergenic 55.0%
GCTGGAACTGTAGCTTCAGC+AGG - chr1_2:69604410-69604429 None:intergenic 55.0%
GGTGCAGGTACATTGGAAGC+TGG - chr1_2:69604428-69604447 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_2 gene 69603132 69604735 69603132 ID=Msa0088520;Name=Msa0088520
chr1_2 mRNA 69603132 69604735 69603132 ID=Msa0088520-mRNA-1;Parent=Msa0088520;Name=Msa0088520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=140|1|1|1|1|1|2|197|185
chr1_2 exon 69603132 69603440 69603132 ID=Msa0088520-mRNA-1:exon:23914;Parent=Msa0088520-mRNA-1
chr1_2 exon 69604150 69604735 69604150 ID=Msa0088520-mRNA-1:exon:23915;Parent=Msa0088520-mRNA-1
chr1_2 five_prime_UTR 69603132 69603271 69603132 ID=Msa0088520-mRNA-1:five_prime_utr;Parent=Msa0088520-mRNA-1
chr1_2 CDS 69603272 69603440 69603272 ID=Msa0088520-mRNA-1:cds;Parent=Msa0088520-mRNA-1
chr1_2 CDS 69604150 69604538 69604150 ID=Msa0088520-mRNA-1:cds;Parent=Msa0088520-mRNA-1
chr1_2 three_prime_UTR 69604539 69604735 69604539 ID=Msa0088520-mRNA-1:three_prime_utr;Parent=Msa0088520-mRNA-1
Gene Sequence

>Msa0088520

ATGGCTTTGGTAGAAAGAGCTATGGTTTTGTCTATAATGATGGTAGCTATGCAGATTTCATATGCAGCTGTATACAAGGTTGGTGATTCTGCTGGCTGGACTACACTTGGTAACATTGACTACAAAAAATGGGCTGCTACCAAGAACTTCCATCTTGGTGACACTATTATATTTGAATATAGTGCAAAATTCCACAATGTGATGCGAGTGACACATGCCATGTACAAGTCATGCAATGCATCATCACCAATTGCAACATTCACTACTGGAAATGACACCATAAAGATAACAAATCATGGTCATCACTTCTTCTTTTGTGGTGTACCTGGACACTGTCAAGCAGGACAGAAGGTTGATATTAACGTGCTCAAAGTTTCTGTAGCTGCTTCACCTGCTCCATCATCTTCACCTTCAGCATTGGCTTCTCCTGCTGAAGCTACAGTTCCAGCTTCCAATGTACCTGCACCTTCTCCTAGTAATGCTGCACCACTGAAATGTATTGCTTTAAAAATGATGCTGGCTTTTTGGGCCATGCAATTTCTTGCTGTTAATTTCTAG
Protein sequence

>Msa0088520

MALVERAMVLSIMMVAMQISYAAVYKVGDSAGWTTLGNIDYKKWAATKNFHLGDTIIFEYSAKFHNVMRVTHAMYKSCNASSPIATFTTGNDTIKITNHGHHFFFCGVPGHCQAGQKVDINVLKVSVAASPAPSSSPSALASPAEATVPASNVPAPSPSNAAPLKCIALKMMLAFWAMQFLAVNF*