AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0110630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0110630 MtrunA17_Chr1g0168531 99.754 406 1 0 1 406 46 451 0.0 824
Msa0110630 MtrunA17_Chr5g0407811 95.750 400 17 0 7 406 9 408 0.0 746
Msa0110630 MtrunA17_Chr1g0181761 93.812 404 25 0 3 406 2 405 0.0 738
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0110630 AT3G09630.1 82.512 406 71 0 1 406 1 406 0.0 692
Msa0110630 AT3G09630.2 82.266 406 71 1 1 406 1 405 0.0 686
Msa0110630 AT5G02870.1 82.266 406 72 0 1 406 2 407 0.0 684
Msa0110630 AT5G02870.2 82.020 406 72 1 1 406 2 406 0.0 677

Find 138 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTACAAACAAGTATGATTT+TGG 0.165926 1_3:-23565408 None:intergenic
AAAGGACCGTTGATTGTTTA+TGG 0.172085 1_3:+23563076 Msa0110630:CDS
CGACCAGAATCTGGCGTAAA+TGG 0.252089 1_3:+23562774 Msa0110630:CDS
CCTGAGTTTCCTCTTGTTGT+AGG 0.252761 1_3:+23562905 Msa0110630:CDS
CGAAAGCAGTTAAAGCATTT+AGG 0.283748 1_3:+23563110 Msa0110630:CDS
GAGAAACTTGATTCGATTTA+CGG 0.289997 1_3:+23563238 Msa0110630:CDS
GTGCCATTTACGCCAGATTC+TGG 0.296253 1_3:-23562777 None:intergenic
CTTGCACCTGGTGGGCATTT+GGG 0.316137 1_3:+23563184 Msa0110630:CDS
TTGTTCTTGCTCGTGGTCAT+AGG 0.318133 1_3:+23562870 Msa0110630:CDS
CCCATGACTCGGCGGAGGTT+TGG 0.322077 1_3:-23562643 None:intergenic
CGCTTAACATCCTTCTTAAT+CGG 0.329357 1_3:-23563376 None:intergenic
ACTTGCACCTGGTGGGCATT+TGG 0.330852 1_3:+23563183 Msa0110630:CDS
GGAGGAAGCTTCTGCCATTA+AGG 0.333983 1_3:+23565004 Msa0110630:intron
AGGTTTGGTGGCCGGCTTTA+CGG 0.335499 1_3:-23562628 None:intergenic
TCCCGTATTCCTCGTGTTGC+CGG 0.340192 1_3:+23562683 Msa0110630:CDS
ATGGACAAAGTTAACAATAT+CGG 0.355207 1_3:-23562564 None:intergenic
TGGTAGGGTGGTTTGTGAAT+CGG 0.366460 1_3:-23562510 None:intergenic
CGTGCTGGTCAAGCTGCTTT+CGG 0.369434 1_3:+23562719 Msa0110630:CDS
AACAAAATTTCACCGCTGTC+TGG 0.376567 1_3:-23565261 None:intergenic
ATTCCTCGTGTTGCCGGAGG+TGG 0.382483 1_3:+23562689 Msa0110630:CDS
ATTTGGGTAGGTTTGTTGTT+TGG 0.382908 1_3:+23563200 Msa0110630:CDS
TGTTTGCTCCGACCAGAATC+TGG 0.383149 1_3:+23562765 Msa0110630:CDS
AGAAACTTGATTCGATTTAC+GGG 0.384012 1_3:+23563239 Msa0110630:CDS
GAAGAGGAAGACTGTTTCTA+AGG 0.386500 1_3:+23563534 Msa0110630:CDS
GACGAGGGGTCTTGCGGCGG+CGG 0.388200 1_3:-23562456 None:intergenic
CTCTTAGCAGTCTTAGCATA+AGG 0.391556 1_3:-23563454 None:intergenic
GTTTGTTGTTTGGACTAAAT+CGG 0.397101 1_3:+23563210 Msa0110630:CDS
CGGCGGCGGAAGCCATTGCT+AGG 0.402539 1_3:-23562442 None:intergenic
GGCTGACGGCGTACGGTTGT+CGG 0.414221 1_3:-23562607 None:intergenic
AGCACGGTGGGTCCCACCTC+CGG 0.414888 1_3:-23562702 None:intergenic
GACAACTTCTCCAAGTGGTT+GGG 0.425629 1_3:+23565083 Msa0110630:CDS
CCATGTCGGAGTCGAGTGTT+TGG 0.427372 1_3:-23562484 None:intergenic
CTCGGCGGAGGTTTGGTGGC+CGG 0.427401 1_3:-23562636 None:intergenic
ATAACATTAAGATTCTTAAG+AGG 0.432087 1_3:-23563418 None:intergenic
CCAAACCTCCGCCGAGTCAT+GGG 0.437310 1_3:+23562643 Msa0110630:CDS
GCATTACATCGGGGATTGGT+AGG 0.449379 1_3:-23562526 None:intergenic
TGCTGAGGCTGAGAGGGTTA+AGG 0.451803 1_3:+23563489 Msa0110630:CDS
GCTGACGGCGTACGGTTGTC+GGG 0.457389 1_3:-23562606 None:intergenic
ATTGAAGCTCGCATTACATC+GGG 0.459128 1_3:-23562536 None:intergenic
TTTAACTGCTTTCGCACCTT+CGG 0.461535 1_3:-23563101 None:intergenic
ACCAAACCTCCGCCGAGTCA+TGG 0.466870 1_3:+23562642 Msa0110630:CDS
GAGAAAGCGAAAGCGAGTCT+TGG 0.467857 1_3:+23563004 Msa0110630:CDS
TCCGCCGAGTCATGGGGAAC+CGG 0.468319 1_3:+23562650 Msa0110630:CDS
AGAAAGCGAAAGCGAGTCTT+GGG 0.468700 1_3:+23563005 Msa0110630:CDS
TGGACAAAGTTAACAATATC+GGG 0.471093 1_3:-23562563 None:intergenic
CTCCGGCAACACGAGGAATA+CGG 0.471401 1_3:-23562685 None:intergenic
CACCTGGTGGGCATTTGGGT+AGG 0.482518 1_3:+23563188 Msa0110630:CDS
CCTTCGCTTGTTCTTGCTCG+TGG 0.487815 1_3:+23562863 Msa0110630:CDS
ATGAGGTGCAGAGTGTTGTT+AGG 0.491829 1_3:+23563353 Msa0110630:CDS
GCTTCTGCCATTAAGGCAGC+TGG 0.495195 1_3:+23565011 Msa0110630:CDS
GCTTTACGGCTGACGGCGTA+CGG 0.496325 1_3:-23562614 None:intergenic
TGAGAGGGTTAAGGCTAAGA+AGG 0.498124 1_3:+23563498 Msa0110630:CDS
TGACAACTTCTCCAAGTGGT+TGG 0.498943 1_3:+23565082 Msa0110630:CDS
TACATCGGGGATTGGTAGGG+TGG 0.502773 1_3:-23562522 None:intergenic
TGTACTTTGCATCACCCACT+AGG 0.507059 1_3:+23565425 Msa0110630:three_prime_UTR
AACACGAGGAATACGGGAAA+CGG 0.507528 1_3:-23562678 None:intergenic
CTTTGCTTGCTGAGGCTGAG+AGG 0.509740 1_3:+23563482 Msa0110630:CDS
TGTTTCAGCTATCGCTGCTT+CGG 0.510115 1_3:+23562835 Msa0110630:CDS
ATGACTCGGCGGAGGTTTGG+TGG 0.511760 1_3:-23562640 None:intergenic
TGTTGTTAGGCCGATTAAGA+AGG 0.511770 1_3:+23563366 Msa0110630:CDS
AAAAGCTTGGTACAACACTA+TGG 0.513409 1_3:+23565034 Msa0110630:CDS
CATTACATCGGGGATTGGTA+GGG 0.514894 1_3:-23562525 None:intergenic
GCTCGCATTACATCGGGGAT+TGG 0.515117 1_3:-23562530 None:intergenic
TTTGCTTGCTGAGGCTGAGA+GGG 0.515410 1_3:+23563483 Msa0110630:CDS
GGAGGTGGGACCCACCGTGC+TGG 0.518181 1_3:+23562704 Msa0110630:CDS
TTCATGATGATTGTATTCGC+TGG 0.521226 1_3:+23565133 Msa0110630:three_prime_UTR
TCCGGCAACACGAGGAATAC+GGG 0.522342 1_3:-23562684 None:intergenic
GAGGATGGCTTTGCTTGCTG+AGG 0.523283 1_3:+23563474 Msa0110630:CDS
GACGGTGACGAGGGGTCTTG+CGG 0.525418 1_3:-23562462 None:intergenic
TTCTAAAAGGTTGCCCTAGT+GGG 0.529418 1_3:-23565439 None:intergenic
GATTGAAGCTCGCATTACAT+CGG 0.530724 1_3:-23562537 None:intergenic
CGTAGGTATATATCGCGAAA+AGG 0.530754 1_3:+23563058 Msa0110630:CDS
AGCGAGTCTTGGGATTCGAC+CGG 0.531495 1_3:+23563015 Msa0110630:CDS
AACCTACCCAAATGCCCACC+AGG 0.531845 1_3:-23563190 None:intergenic
AGGAGAAACTTGACAAGAAG+AGG 0.532236 1_3:+23563518 Msa0110630:CDS
GGCGGCGGAAGCCATTGCTA+GGG 0.532782 1_3:-23562441 None:intergenic
CGAGTGTTTGGACGGTGACG+AGG 0.534152 1_3:-23562472 None:intergenic
TACGCCGTCAGCCGTAAAGC+CGG 0.535611 1_3:+23562617 Msa0110630:CDS
GTTGTAGGTGATTCAGCTGA+AGG 0.538478 1_3:+23562920 Msa0110630:CDS
GCGAGTCTTGGGATTCGACC+GGG 0.544565 1_3:+23563016 Msa0110630:CDS
GGTTCCCCATGACTCGGCGG+AGG 0.548363 1_3:-23562648 None:intergenic
TGGTTAATTCTGATTTGACT+AGG 0.548923 1_3:+23563317 Msa0110630:CDS
AATACGGGAAACGGCACGAC+CGG 0.550446 1_3:-23562669 None:intergenic
TGAAGGAGTAGAGAAAACTA+AGG 0.551422 1_3:+23562937 Msa0110630:CDS
AGCAGCGATAGCTGAAACAA+CGG 0.554324 1_3:-23562831 None:intergenic
GCTGAATCACCTACAACAAG+AGG 0.554499 1_3:-23562914 None:intergenic
TTAAGGCAGCTGGAAAAGCT+TGG 0.555525 1_3:+23565021 Msa0110630:CDS
TTCCTCGTGTTGCCGGAGGT+GGG 0.556101 1_3:+23562690 Msa0110630:CDS
CTTGGGATTCGACCGGGGAA+AGG 0.556923 1_3:+23563022 Msa0110630:CDS
AGCAGCTTGACCAGCACGGT+GGG 0.557276 1_3:-23562714 None:intergenic
CTGTGAAACACCCAACCACT+TGG 0.559270 1_3:-23565093 None:intergenic
TTGTGAATCGGTGGCCATGT+CGG 0.562473 1_3:-23562498 None:intergenic
GAATTAACCATCTTAGCCCT+AGG 0.569796 1_3:-23563304 None:intergenic
CATTTACGCCAGATTCTGGT+CGG 0.570290 1_3:-23562773 None:intergenic
TTACGCATCTTACCTTTCCC+CGG 0.571177 1_3:-23563034 None:intergenic
ACTGGGAATTTACTCTACAC+TGG 0.571321 1_3:-23565495 None:intergenic
TTGATTGTTTATGGTACCGA+AGG 0.573110 1_3:+23563085 Msa0110630:CDS
GTGGCCGGCTTTACGGCTGA+CGG 0.574691 1_3:-23562621 None:intergenic
AATCATCATGAACATCAAGA+TGG 0.577020 1_3:-23565122 None:intergenic
GTCGGAGTCGAGTGTTTGGA+CGG 0.584029 1_3:-23562480 None:intergenic
GAATCTGGCGTAAATGGCAC+CGG 0.584056 1_3:+23562780 Msa0110630:CDS
CTTATGCTAAGACTGCTAAG+AGG 0.584978 1_3:+23563455 Msa0110630:CDS
TTGGTACAACACTATGGTCT+CGG 0.586235 1_3:+23565040 Msa0110630:CDS
GTTCTAAAAGGTTGCCCTAG+TGG 0.587381 1_3:-23565440 None:intergenic
GGTGACGAGGGGTCTTGCGG+CGG 0.587727 1_3:-23562459 None:intergenic
TGCTAAGACTGCTAAGAGGA+TGG 0.588122 1_3:+23563459 Msa0110630:CDS
GAGTGTTTGGACGGTGACGA+GGG 0.598147 1_3:-23562471 None:intergenic
GTCCCACCTCCGGCAACACG+AGG 0.600871 1_3:-23562692 None:intergenic
AAACAAAACACTAATACGAA+AGG 0.603474 1_3:-23565169 None:intergenic
CCTACAACAAGAGGAAACTC+AGG 0.611282 1_3:-23562905 None:intergenic
CGTATTCCTCGTGTTGCCGG+AGG 0.611699 1_3:+23562686 Msa0110630:CDS
TATTGAAACTTGCACCTGGT+GGG 0.615402 1_3:+23563176 Msa0110630:CDS
AAGGTAAGATGCGTAACCGT+AGG 0.626245 1_3:+23563041 Msa0110630:CDS
AATTTGACAACTTCTCCAAG+TGG 0.627357 1_3:+23565078 Msa0110630:CDS
TTGAGATTACTAATGTTGAG+AGG 0.628464 1_3:+23563146 Msa0110630:CDS
ACGACCGGTTCCCCATGACT+CGG 0.634902 1_3:-23562654 None:intergenic
CTATTGAAACTTGCACCTGG+TGG 0.636819 1_3:+23563175 Msa0110630:CDS
TCGGTACCATAAACAATCAA+CGG 0.638403 1_3:-23563082 None:intergenic
CCAAACACTCGACTCCGACA+TGG 0.639783 1_3:+23562484 Msa0110630:CDS
GTACTTTGCATCACCCACTA+GGG 0.640151 1_3:+23565426 Msa0110630:three_prime_UTR
TTCGGTAACATGTGCCGTGG+TGG 0.641371 1_3:+23562737 Msa0110630:CDS
ACGATAGAAAACCCTAGCAA+TGG 0.641909 1_3:+23562430 Msa0110630:exon
TAGGGTGGTTTGTGAATCGG+TGG 0.642809 1_3:-23562507 None:intergenic
CAAACCTCCGCCGAGTCATG+GGG 0.643143 1_3:+23562644 Msa0110630:CDS
AAAGCATTTAGGAATATACC+TGG 0.643999 1_3:+23563121 Msa0110630:CDS
CAAAGTTAACAATATCGGGA+CGG 0.646080 1_3:-23562559 None:intergenic
TAGGATTATTAACAGTGATG+AGG 0.647484 1_3:+23563336 Msa0110630:CDS
AATCTATTGAAACTTGCACC+TGG 0.652549 1_3:+23563172 Msa0110630:CDS
CGAAAGCAGCTTGACCAGCA+CGG 0.654853 1_3:-23562718 None:intergenic
AGTGTTTGGACGGTGACGAG+GGG 0.664474 1_3:-23562470 None:intergenic
CCACGAGCAAGAACAAGCGA+AGG 0.669367 1_3:-23562863 None:intergenic
TTGAAGCTCGCATTACATCG+GGG 0.675972 1_3:-23562535 None:intergenic
ACATTAGTAATCTCAACACC+AGG 0.679853 1_3:-23563139 None:intergenic
CGAGTCTTGGGATTCGACCG+GGG 0.689790 1_3:+23563017 Msa0110630:CDS
AAGCAGCTTGACCAGCACGG+TGG 0.692670 1_3:-23562715 None:intergenic
ACCGGTTCCCCATGACTCGG+CGG 0.694101 1_3:-23562651 None:intergenic
GCTTTCGGTAACATGTGCCG+TGG 0.703272 1_3:+23562734 Msa0110630:CDS
GAGCAAACATACGACCACCA+CGG 0.745806 1_3:-23562751 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTTTATGTTTTATTATT+AGG + chr1_3:23564926-23564945 Msa0110630:intron 10.0%
!!! AAAAAAAATCTAGTTCTAAA+AGG - chr1_3:23565455-23565474 None:intergenic 15.0%
!!! GATTTTTTTTTTCATTTTCT+CGG + chr1_3:23565465-23565484 Msa0110630:three_prime_UTR 15.0%
!!! ATTAGTGTTTTGAAAAAGAT+TGG + chr1_3:23562965-23562984 Msa0110630:CDS 20.0%
!! ATAACATTAAGATTCTTAAG+AGG - chr1_3:23563421-23563440 None:intergenic 20.0%
!! ATTCAAATCAAAATGCAATA+AGG - chr1_3:23563700-23563719 None:intergenic 20.0%
!! AATGATAGAAATTAAAAACC+AGG - chr1_3:23564145-23564164 None:intergenic 20.0%
!! AATCATATTCCATTTACAAA+AGG + chr1_3:23564482-23564501 Msa0110630:intron 20.0%
!! TAGTAAGTTAGATAGAAATA+TGG + chr1_3:23564514-23564533 Msa0110630:intron 20.0%
!!! TATTAGTGTTTTGTTTTGTA+TGG + chr1_3:23565176-23565195 Msa0110630:three_prime_UTR 20.0%
! ATGGACAAAGTTAACAATAT+CGG - chr1_3:23562567-23562586 None:intergenic 25.0%
!!! AGTTTTTAGAAATGTTGGAA+TGG - chr1_3:23562586-23562605 None:intergenic 25.0%
!!! TGTTTTGATGTTTCATTTTG+TGG + chr1_3:23563628-23563647 Msa0110630:intron 25.0%
!!! GTTTTGATGTTTCATTTTGT+GGG + chr1_3:23563629-23563648 Msa0110630:intron 25.0%
! TTCTTCAAATGATGCAATTA+AGG + chr1_3:23564408-23564427 Msa0110630:intron 25.0%
! TTTCCATTTACATTTCTGTT+TGG + chr1_3:23564627-23564646 Msa0110630:intron 25.0%
! ATACCAAACAGAAATGTAAA+TGG - chr1_3:23564633-23564652 None:intergenic 25.0%
!! TATGTCTTTATGACAACTTT+TGG + chr1_3:23564692-23564711 Msa0110630:intron 25.0%
! ACATAAAACAATTACAATGC+AGG - chr1_3:23564917-23564936 None:intergenic 25.0%
! ATTTGTCTCAATATTGTTCA+TGG + chr1_3:23564950-23564969 Msa0110630:intron 25.0%
!!! ACATTATTTTCCTTTGTTTC+AGG + chr1_3:23564983-23565002 Msa0110630:intron 25.0%
! AAACAAAACACTAATACGAA+AGG - chr1_3:23565172-23565191 None:intergenic 25.0%
!! AGTACAAACAAGTATGATTT+TGG - chr1_3:23565411-23565430 None:intergenic 25.0%
TGGACAAAGTTAACAATATC+GGG - chr1_3:23562566-23562585 None:intergenic 30.0%
!!! TCTGATTCACATTGATTTTC+CGG - chr1_3:23562802-23562821 None:intergenic 30.0%
! AAAGCATTTAGGAATATACC+TGG + chr1_3:23563121-23563140 Msa0110630:CDS 30.0%
TTGAGATTACTAATGTTGAG+AGG + chr1_3:23563146-23563165 Msa0110630:CDS 30.0%
!! GTTTGTTGTTTGGACTAAAT+CGG + chr1_3:23563210-23563229 Msa0110630:CDS 30.0%
! GAGAAACTTGATTCGATTTA+CGG + chr1_3:23563238-23563257 Msa0110630:CDS 30.0%
! AGAAACTTGATTCGATTTAC+GGG + chr1_3:23563239-23563258 Msa0110630:CDS 30.0%
! ATTTACGGGACTTTTGATAA+GGG + chr1_3:23563253-23563272 Msa0110630:CDS 30.0%
!! AAAAAGGGTATGTTTTACCT+AGG + chr1_3:23563287-23563306 Msa0110630:CDS 30.0%
!! AAAAGGGTATGTTTTACCTA+GGG + chr1_3:23563288-23563307 Msa0110630:CDS 30.0%
! TGGTTAATTCTGATTTGACT+AGG + chr1_3:23563317-23563336 Msa0110630:CDS 30.0%
TAGGATTATTAACAGTGATG+AGG + chr1_3:23563336-23563355 Msa0110630:CDS 30.0%
! GCTTTTTGTGTACATCATTT+AGG - chr1_3:23563895-23563914 None:intergenic 30.0%
!! ATAGATGGTGGATATAGAAT+AGG + chr1_3:23564225-23564244 Msa0110630:intron 30.0%
TGTGATTTATTGTCAACTAG+CGG + chr1_3:23564260-23564279 Msa0110630:intron 30.0%
CTATCTAACTTACTAATCTC+TGG - chr1_3:23564509-23564528 None:intergenic 30.0%
TTATAACGTCGTTTGATCAA+TGG + chr1_3:23564551-23564570 Msa0110630:intron 30.0%
CAATAGAGGATAATCAAAAG+TGG - chr1_3:23564754-23564773 None:intergenic 30.0%
CATTAACTGCAATTCAATAG+AGG - chr1_3:23564768-23564787 None:intergenic 30.0%
!! TTATTTTCCTTTGTTTCAGG+AGG + chr1_3:23564986-23565005 Msa0110630:intron 30.0%
AATCATCATGAACATCAAGA+TGG - chr1_3:23565125-23565144 None:intergenic 30.0%
! TTACCTGAACGGAATATTTT+TGG + chr1_3:23565199-23565218 Msa0110630:three_prime_UTR 30.0%
! TACCTGAACGGAATATTTTT+GGG + chr1_3:23565200-23565219 Msa0110630:three_prime_UTR 30.0%
!! TTTTATTGTTTTGCGAGTTG+AGG + chr1_3:23565224-23565243 Msa0110630:three_prime_UTR 30.0%
CAAAGTTAACAATATCGGGA+CGG - chr1_3:23562562-23562581 None:intergenic 35.0%
!! TCGGGAGTTTTTAGAAATGT+TGG - chr1_3:23562591-23562610 None:intergenic 35.0%
TGAAGGAGTAGAGAAAACTA+AGG + chr1_3:23562937-23562956 Msa0110630:CDS 35.0%
! CTTTTCGCGATATATACCTA+CGG - chr1_3:23563060-23563079 None:intergenic 35.0%
! AAAGGACCGTTGATTGTTTA+TGG + chr1_3:23563076-23563095 Msa0110630:CDS 35.0%
TCGGTACCATAAACAATCAA+CGG - chr1_3:23563085-23563104 None:intergenic 35.0%
! TTGATTGTTTATGGTACCGA+AGG + chr1_3:23563085-23563104 Msa0110630:CDS 35.0%
! CGAAAGCAGTTAAAGCATTT+AGG + chr1_3:23563110-23563129 Msa0110630:CDS 35.0%
ACATTAGTAATCTCAACACC+AGG - chr1_3:23563142-23563161 None:intergenic 35.0%
AATCTATTGAAACTTGCACC+TGG + chr1_3:23563172-23563191 Msa0110630:CDS 35.0%
!! ATTTGGGTAGGTTTGTTGTT+TGG + chr1_3:23563200-23563219 Msa0110630:CDS 35.0%
! GATTTACGGGACTTTTGATA+AGG + chr1_3:23563252-23563271 Msa0110630:CDS 35.0%
AAGGGTAGTGAGAAGAAAAA+AGG + chr1_3:23563271-23563290 Msa0110630:CDS 35.0%
AGGGTAGTGAGAAGAAAAAA+GGG + chr1_3:23563272-23563291 Msa0110630:CDS 35.0%
CGCTTAACATCCTTCTTAAT+CGG - chr1_3:23563379-23563398 None:intergenic 35.0%
AACTGGTTGTGCAATTTCAA+AGG - chr1_3:23563763-23563782 None:intergenic 35.0%
! TTTGAAATTGCACAACCAGT+TGG + chr1_3:23563762-23563781 Msa0110630:intron 35.0%
! CGAACTGAATTTTACTTTGC+TGG + chr1_3:23564062-23564081 Msa0110630:intron 35.0%
GTGCATAGTGTAATGTTGTT+TGG + chr1_3:23564108-23564127 Msa0110630:intron 35.0%
! TGTTTGGTTTAGATGTAACC+TGG + chr1_3:23564124-23564143 Msa0110630:intron 35.0%
AGTTAGTGCTGTCAAATAGA+TGG + chr1_3:23564210-23564229 Msa0110630:intron 35.0%
TTACTGCAACGTATCTTAGT+GGG + chr1_3:23564285-23564304 Msa0110630:intron 35.0%
TTGGCATTGCTATGAAACTA+GGG - chr1_3:23564367-23564386 None:intergenic 35.0%
ATTGGCATTGCTATGAAACT+AGG - chr1_3:23564368-23564387 None:intergenic 35.0%
! ATCTCTGGACCTTTTGTAAA+TGG - chr1_3:23564494-23564513 None:intergenic 35.0%
GATAATCAAAAGTGGAGCAT+CGG - chr1_3:23564746-23564765 None:intergenic 35.0%
AAAAGCTTGGTACAACACTA+TGG + chr1_3:23565034-23565053 Msa0110630:CDS 35.0%
AATTTGACAACTTCTCCAAG+TGG + chr1_3:23565078-23565097 Msa0110630:CDS 35.0%
TTCATGATGATTGTATTCGC+TGG + chr1_3:23565133-23565152 Msa0110630:three_prime_UTR 35.0%
! GTTTTGTATGGTTACCTGAA+CGG + chr1_3:23565188-23565207 Msa0110630:three_prime_UTR 35.0%
!! ACCTGAACGGAATATTTTTG+GGG + chr1_3:23565201-23565220 Msa0110630:three_prime_UTR 35.0%
ACGATAGAAAACCCTAGCAA+TGG + chr1_3:23562430-23562449 Msa0110630:exon 40.0%
ATTGAAGCTCGCATTACATC+GGG - chr1_3:23562539-23562558 None:intergenic 40.0%
GATTGAAGCTCGCATTACAT+CGG - chr1_3:23562540-23562559 None:intergenic 40.0%
CGTAGGTATATATCGCGAAA+AGG + chr1_3:23563058-23563077 Msa0110630:CDS 40.0%
TTTAACTGCTTTCGCACCTT+CGG - chr1_3:23563104-23563123 None:intergenic 40.0%
TATTGAAACTTGCACCTGGT+GGG + chr1_3:23563176-23563195 Msa0110630:CDS 40.0%
! TGTTTTACCTAGGGCTAAGA+TGG + chr1_3:23563297-23563316 Msa0110630:CDS 40.0%
GAATTAACCATCTTAGCCCT+AGG - chr1_3:23563307-23563326 None:intergenic 40.0%
TGTTGTTAGGCCGATTAAGA+AGG + chr1_3:23563366-23563385 Msa0110630:CDS 40.0%
CTCTTAGCAGTCTTAGCATA+AGG - chr1_3:23563457-23563476 None:intergenic 40.0%
CTTATGCTAAGACTGCTAAG+AGG + chr1_3:23563455-23563474 Msa0110630:CDS 40.0%
AGGAGAAACTTGACAAGAAG+AGG + chr1_3:23563518-23563537 Msa0110630:CDS 40.0%
!! GAAGAGGAAGACTGTTTCTA+AGG + chr1_3:23563534-23563553 Msa0110630:CDS 40.0%
AACCAGTTGGACTCTCTATT+TGG + chr1_3:23563775-23563794 Msa0110630:intron 40.0%
! TCCTCTAGTGTAAGGTATCA+CGG + chr1_3:23563933-23563952 Msa0110630:intron 40.0%
TAGTGCTGTCAAATAGATGG+TGG + chr1_3:23564213-23564232 Msa0110630:intron 40.0%
GTTACTGCAACGTATCTTAG+TGG + chr1_3:23564284-23564303 Msa0110630:intron 40.0%
ACCAATCCGTATGTGACTAA+GGG - chr1_3:23564586-23564605 None:intergenic 40.0%
AACCAATCCGTATGTGACTA+AGG - chr1_3:23564587-23564606 None:intergenic 40.0%
CAATGCAGGAAATAGAGCAA+AGG - chr1_3:23564903-23564922 None:intergenic 40.0%
TTGGTACAACACTATGGTCT+CGG + chr1_3:23565040-23565059 Msa0110630:CDS 40.0%
ACCCCAAAAATATTCCGTTC+AGG - chr1_3:23565205-23565224 None:intergenic 40.0%
! ATTGCACATTTTCCAGACAG+CGG + chr1_3:23565249-23565268 Msa0110630:three_prime_UTR 40.0%
AACAAAATTTCACCGCTGTC+TGG - chr1_3:23565264-23565283 None:intergenic 40.0%
! TTCTAAAAGGTTGCCCTAGT+GGG - chr1_3:23565442-23565461 None:intergenic 40.0%
! TGGTAGGGTGGTTTGTGAAT+CGG - chr1_3:23562513-23562532 None:intergenic 45.0%
! CATTACATCGGGGATTGGTA+GGG - chr1_3:23562528-23562547 None:intergenic 45.0%
TTGAAGCTCGCATTACATCG+GGG - chr1_3:23562538-23562557 None:intergenic 45.0%
AACACGAGGAATACGGGAAA+CGG - chr1_3:23562681-23562700 None:intergenic 45.0%
CATTTACGCCAGATTCTGGT+CGG - chr1_3:23562776-23562795 None:intergenic 45.0%
AGCAGCGATAGCTGAAACAA+CGG - chr1_3:23562834-23562853 None:intergenic 45.0%
TGTTTCAGCTATCGCTGCTT+CGG + chr1_3:23562835-23562854 Msa0110630:CDS 45.0%
TTGTTCTTGCTCGTGGTCAT+AGG + chr1_3:23562870-23562889 Msa0110630:CDS 45.0%
CCTACAACAAGAGGAAACTC+AGG - chr1_3:23562908-23562927 None:intergenic 45.0%
! CCTGAGTTTCCTCTTGTTGT+AGG + chr1_3:23562905-23562924 Msa0110630:CDS 45.0%
GCTGAATCACCTACAACAAG+AGG - chr1_3:23562917-23562936 None:intergenic 45.0%
GTTGTAGGTGATTCAGCTGA+AGG + chr1_3:23562920-23562939 Msa0110630:CDS 45.0%
AGAAAGCGAAAGCGAGTCTT+GGG + chr1_3:23563005-23563024 Msa0110630:CDS 45.0%
TTACGCATCTTACCTTTCCC+CGG - chr1_3:23563037-23563056 None:intergenic 45.0%
AAGGTAAGATGCGTAACCGT+AGG + chr1_3:23563041-23563060 Msa0110630:CDS 45.0%
CTATTGAAACTTGCACCTGG+TGG + chr1_3:23563175-23563194 Msa0110630:CDS 45.0%
!! ATGAGGTGCAGAGTGTTGTT+AGG + chr1_3:23563353-23563372 Msa0110630:CDS 45.0%
TGCTAAGACTGCTAAGAGGA+TGG + chr1_3:23563459-23563478 Msa0110630:CDS 45.0%
TGAGAGGGTTAAGGCTAAGA+AGG + chr1_3:23563498-23563517 Msa0110630:CDS 45.0%
GACCAAATAGAGAGTCCAAC+TGG - chr1_3:23563780-23563799 None:intergenic 45.0%
ACACTAGAGGATGTCTTGCT+TGG - chr1_3:23563924-23563943 None:intergenic 45.0%
GCAAGACATCCTCTAGTGTA+AGG + chr1_3:23563925-23563944 Msa0110630:intron 45.0%
! CCTCTAGTGTAAGGTATCAC+GGG + chr1_3:23563934-23563953 Msa0110630:intron 45.0%
CACTATGCACAGCACATTCT+AGG - chr1_3:23564098-23564117 None:intergenic 45.0%
CACAACAGAACGAAGCACAT+TGG - chr1_3:23564386-23564405 None:intergenic 45.0%
CAATCCGTATGTGACTAAGG+GGG - chr1_3:23564584-23564603 None:intergenic 45.0%
CCAATCCGTATGTGACTAAG+GGG - chr1_3:23564585-23564604 None:intergenic 45.0%
!! GTCACATACGGATTGGTTGT+TGG + chr1_3:23564589-23564608 Msa0110630:intron 45.0%
!! TGGAGCATCGGTTGCAATTA+AGG - chr1_3:23564734-23564753 None:intergenic 45.0%
GAAGCTTCCTCCTGAAACAA+AGG - chr1_3:23564996-23565015 None:intergenic 45.0%
! AGCTTTTCCAGCTGCCTTAA+TGG - chr1_3:23565021-23565040 None:intergenic 45.0%
TTAAGGCAGCTGGAAAAGCT+TGG + chr1_3:23565021-23565040 Msa0110630:CDS 45.0%
TGACAACTTCTCCAAGTGGT+TGG + chr1_3:23565082-23565101 Msa0110630:CDS 45.0%
GACAACTTCTCCAAGTGGTT+GGG + chr1_3:23565083-23565102 Msa0110630:CDS 45.0%
TGTACTTTGCATCACCCACT+AGG + chr1_3:23565425-23565444 Msa0110630:three_prime_UTR 45.0%
GTACTTTGCATCACCCACTA+GGG + chr1_3:23565426-23565445 Msa0110630:three_prime_UTR 45.0%
!! GTTCTAAAAGGTTGCCCTAG+TGG - chr1_3:23565443-23565462 None:intergenic 45.0%
TTGTGAATCGGTGGCCATGT+CGG - chr1_3:23562501-23562520 None:intergenic 50.0%
! TAGGGTGGTTTGTGAATCGG+TGG - chr1_3:23562510-23562529 None:intergenic 50.0%
! GCATTACATCGGGGATTGGT+AGG - chr1_3:23562529-23562548 None:intergenic 50.0%
GAGCAAACATACGACCACCA+CGG - chr1_3:23562754-23562773 None:intergenic 50.0%
TGTTTGCTCCGACCAGAATC+TGG + chr1_3:23562765-23562784 Msa0110630:CDS 50.0%
CGACCAGAATCTGGCGTAAA+TGG + chr1_3:23562774-23562793 Msa0110630:CDS 50.0%
!! GTGCCATTTACGCCAGATTC+TGG - chr1_3:23562780-23562799 None:intergenic 50.0%
GAATCTGGCGTAAATGGCAC+CGG + chr1_3:23562780-23562799 Msa0110630:CDS 50.0%
GAGAAAGCGAAAGCGAGTCT+TGG + chr1_3:23563004-23563023 Msa0110630:CDS 50.0%
! TTTGCTTGCTGAGGCTGAGA+GGG + chr1_3:23563483-23563502 Msa0110630:CDS 50.0%
CCCGTGATACCTTACACTAG+AGG - chr1_3:23563937-23563956 None:intergenic 50.0%
CGTATGTGACTAAGGGGGAT+CGG - chr1_3:23564579-23564598 None:intergenic 50.0%
CGATCCCCCTTAGTCACATA+CGG + chr1_3:23564577-23564596 Msa0110630:intron 50.0%
CCCCTTAGTCACATACGGAT+TGG + chr1_3:23564582-23564601 Msa0110630:intron 50.0%
GGAGGAAGCTTCTGCCATTA+AGG + chr1_3:23565004-23565023 Msa0110630:intron 50.0%
CTGTGAAACACCCAACCACT+TGG - chr1_3:23565096-23565115 None:intergenic 50.0%
AGTGTTTGGACGGTGACGAG+GGG - chr1_3:23562473-23562492 None:intergenic 55.0%
GAGTGTTTGGACGGTGACGA+GGG - chr1_3:23562474-23562493 None:intergenic 55.0%
GTCGGAGTCGAGTGTTTGGA+CGG - chr1_3:23562483-23562502 None:intergenic 55.0%
CCATGTCGGAGTCGAGTGTT+TGG - chr1_3:23562487-23562506 None:intergenic 55.0%
CCAAACACTCGACTCCGACA+TGG + chr1_3:23562484-23562503 Msa0110630:CDS 55.0%
! TACATCGGGGATTGGTAGGG+TGG - chr1_3:23562525-23562544 None:intergenic 55.0%
GCTCGCATTACATCGGGGAT+TGG - chr1_3:23562533-23562552 None:intergenic 55.0%
!! AGGTTTGGTGGCCGGCTTTA+CGG - chr1_3:23562631-23562650 None:intergenic 55.0%
AATACGGGAAACGGCACGAC+CGG - chr1_3:23562672-23562691 None:intergenic 55.0%
TCCCGTATTCCTCGTGTTGC+CGG + chr1_3:23562683-23562702 Msa0110630:CDS 55.0%
TCCGGCAACACGAGGAATAC+GGG - chr1_3:23562687-23562706 None:intergenic 55.0%
CTCCGGCAACACGAGGAATA+CGG - chr1_3:23562688-23562707 None:intergenic 55.0%
CGAAAGCAGCTTGACCAGCA+CGG - chr1_3:23562721-23562740 None:intergenic 55.0%
! CGTGCTGGTCAAGCTGCTTT+CGG + chr1_3:23562719-23562738 Msa0110630:CDS 55.0%
GCTTTCGGTAACATGTGCCG+TGG + chr1_3:23562734-23562753 Msa0110630:CDS 55.0%
TTCGGTAACATGTGCCGTGG+TGG + chr1_3:23562737-23562756 Msa0110630:CDS 55.0%
CCACGAGCAAGAACAAGCGA+AGG - chr1_3:23562866-23562885 None:intergenic 55.0%
CCTTCGCTTGTTCTTGCTCG+TGG + chr1_3:23562863-23562882 Msa0110630:CDS 55.0%
AGCGAGTCTTGGGATTCGAC+CGG + chr1_3:23563015-23563034 Msa0110630:CDS 55.0%
! ACTTGCACCTGGTGGGCATT+TGG + chr1_3:23563183-23563202 Msa0110630:CDS 55.0%
! CTTGCACCTGGTGGGCATTT+GGG + chr1_3:23563184-23563203 Msa0110630:CDS 55.0%
AACCTACCCAAATGCCCACC+AGG - chr1_3:23563193-23563212 None:intergenic 55.0%
! GAGGATGGCTTTGCTTGCTG+AGG + chr1_3:23563474-23563493 Msa0110630:CDS 55.0%
! CTTTGCTTGCTGAGGCTGAG+AGG + chr1_3:23563482-23563501 Msa0110630:CDS 55.0%
TGCTGAGGCTGAGAGGGTTA+AGG + chr1_3:23563489-23563508 Msa0110630:CDS 55.0%
GCTTCTGCCATTAAGGCAGC+TGG + chr1_3:23565011-23565030 Msa0110630:CDS 55.0%
CGAGTGTTTGGACGGTGACG+AGG - chr1_3:23562475-23562494 None:intergenic 60.0%
GCTTTACGGCTGACGGCGTA+CGG - chr1_3:23562617-23562636 None:intergenic 60.0%
TACGCCGTCAGCCGTAAAGC+CGG + chr1_3:23562617-23562636 Msa0110630:CDS 60.0%
! ATGACTCGGCGGAGGTTTGG+TGG - chr1_3:23562643-23562662 None:intergenic 60.0%
ACCAAACCTCCGCCGAGTCA+TGG + chr1_3:23562642-23562661 Msa0110630:CDS 60.0%
CCAAACCTCCGCCGAGTCAT+GGG + chr1_3:23562643-23562662 Msa0110630:CDS 60.0%
CAAACCTCCGCCGAGTCATG+GGG + chr1_3:23562644-23562663 Msa0110630:CDS 60.0%
! ACGACCGGTTCCCCATGACT+CGG - chr1_3:23562657-23562676 None:intergenic 60.0%
CGTATTCCTCGTGTTGCCGG+AGG + chr1_3:23562686-23562705 Msa0110630:CDS 60.0%
ATTCCTCGTGTTGCCGGAGG+TGG + chr1_3:23562689-23562708 Msa0110630:CDS 60.0%
TTCCTCGTGTTGCCGGAGGT+GGG + chr1_3:23562690-23562709 Msa0110630:CDS 60.0%
AGCAGCTTGACCAGCACGGT+GGG - chr1_3:23562717-23562736 None:intergenic 60.0%
AAGCAGCTTGACCAGCACGG+TGG - chr1_3:23562718-23562737 None:intergenic 60.0%
GCGAGTCTTGGGATTCGACC+GGG + chr1_3:23563016-23563035 Msa0110630:CDS 60.0%
CGAGTCTTGGGATTCGACCG+GGG + chr1_3:23563017-23563036 Msa0110630:CDS 60.0%
CTTGGGATTCGACCGGGGAA+AGG + chr1_3:23563022-23563041 Msa0110630:CDS 60.0%
! CACCTGGTGGGCATTTGGGT+AGG + chr1_3:23563188-23563207 Msa0110630:CDS 60.0%
GGCGGCGGAAGCCATTGCTA+GGG - chr1_3:23562444-23562463 None:intergenic 65.0%
GACGGTGACGAGGGGTCTTG+CGG - chr1_3:23562465-23562484 None:intergenic 65.0%
GCTGACGGCGTACGGTTGTC+GGG - chr1_3:23562609-23562628 None:intergenic 65.0%
GGCTGACGGCGTACGGTTGT+CGG - chr1_3:23562610-23562629 None:intergenic 65.0%
! GTGGCCGGCTTTACGGCTGA+CGG - chr1_3:23562624-23562643 None:intergenic 65.0%
! CCCATGACTCGGCGGAGGTT+TGG - chr1_3:23562646-23562665 None:intergenic 65.0%
TCCGCCGAGTCATGGGGAAC+CGG + chr1_3:23562650-23562669 Msa0110630:CDS 65.0%
! ACCGGTTCCCCATGACTCGG+CGG - chr1_3:23562654-23562673 None:intergenic 65.0%
CGGCGGCGGAAGCCATTGCT+AGG - chr1_3:23562445-23562464 None:intergenic 70.0%
GGTGACGAGGGGTCTTGCGG+CGG - chr1_3:23562462-23562481 None:intergenic 70.0%
!! CTCGGCGGAGGTTTGGTGGC+CGG - chr1_3:23562639-23562658 None:intergenic 70.0%
! GGTTCCCCATGACTCGGCGG+AGG - chr1_3:23562651-23562670 None:intergenic 70.0%
GTCCCACCTCCGGCAACACG+AGG - chr1_3:23562695-23562714 None:intergenic 70.0%
AGCACGGTGGGTCCCACCTC+CGG - chr1_3:23562705-23562724 None:intergenic 70.0%
GACGAGGGGTCTTGCGGCGG+CGG - chr1_3:23562459-23562478 None:intergenic 75.0%
GGAGGTGGGACCCACCGTGC+TGG + chr1_3:23562704-23562723 Msa0110630:CDS 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 23562424 23565513 23562424 ID=Msa0110630;Name=Msa0110630
chr1_3 mRNA 23562424 23565513 23562424 ID=Msa0110630-mRNA-1;Parent=Msa0110630;Name=Msa0110630-mRNA-1;_AED=0.00;_eAED=0.00;_QI=25|1|1|1|1|1|2|395|406
chr1_3 exon 23562424 23563555 23562424 ID=Msa0110630-mRNA-1:exon:8228;Parent=Msa0110630-mRNA-1
chr1_3 exon 23565005 23565513 23565005 ID=Msa0110630-mRNA-1:exon:8229;Parent=Msa0110630-mRNA-1
chr1_3 five_prime_UTR 23562424 23562448 23562424 ID=Msa0110630-mRNA-1:five_prime_utr;Parent=Msa0110630-mRNA-1
chr1_3 CDS 23562449 23563555 23562449 ID=Msa0110630-mRNA-1:cds;Parent=Msa0110630-mRNA-1
chr1_3 CDS 23565005 23565118 23565005 ID=Msa0110630-mRNA-1:cds;Parent=Msa0110630-mRNA-1
chr1_3 three_prime_UTR 23565119 23565513 23565119 ID=Msa0110630-mRNA-1:three_prime_utr;Parent=Msa0110630-mRNA-1
Gene Sequence

>Msa0110630

ATGGCTTCCGCCGCCGCAAGACCCCTCGTCACCGTCCAAACACTCGACTCCGACATGGCCACCGATTCACAAACCACCCTACCAATCCCCGATGTAATGCGAGCTTCAATCCGTCCCGATATTGTTAACTTTGTCCATTCCAACATTTCTAAAAACTCCCGACAACCGTACGCCGTCAGCCGTAAAGCCGGCCACCAAACCTCCGCCGAGTCATGGGGAACCGGTCGTGCCGTTTCCCGTATTCCTCGTGTTGCCGGAGGTGGGACCCACCGTGCTGGTCAAGCTGCTTTCGGTAACATGTGCCGTGGTGGTCGTATGTTTGCTCCGACCAGAATCTGGCGTAAATGGCACCGGAAAATCAATGTGAATCAGAAGCGTTACGCCGTTGTTTCAGCTATCGCTGCTTCGGCGATTCCTTCGCTTGTTCTTGCTCGTGGTCATAGGATTGAAACTGTACCTGAGTTTCCTCTTGTTGTAGGTGATTCAGCTGAAGGAGTAGAGAAAACTAAGGAAGCTATTAGTGTTTTGAAAAAGATTGGAGCTTTTGCTGATGCTGAGAAAGCGAAAGCGAGTCTTGGGATTCGACCGGGGAAAGGTAAGATGCGTAACCGTAGGTATATATCGCGAAAAGGACCGTTGATTGTTTATGGTACCGAAGGTGCGAAAGCAGTTAAAGCATTTAGGAATATACCTGGTGTTGAGATTACTAATGTTGAGAGGTTGAATCTATTGAAACTTGCACCTGGTGGGCATTTGGGTAGGTTTGTTGTTTGGACTAAATCGGCTTTTGAGAAACTTGATTCGATTTACGGGACTTTTGATAAGGGTAGTGAGAAGAAAAAAGGGTATGTTTTACCTAGGGCTAAGATGGTTAATTCTGATTTGACTAGGATTATTAACAGTGATGAGGTGCAGAGTGTTGTTAGGCCGATTAAGAAGGATGTTAAGCGCGCTACGCTTAAGAAGAATCCTCTTAAGAATCTTAATGTTATGCTTAGACTTAATCCTTATGCTAAGACTGCTAAGAGGATGGCTTTGCTTGCTGAGGCTGAGAGGGTTAAGGCTAAGAAGGAGAAACTTGACAAGAAGAGGAAGACTGTTTCTAAGGAGGAAGCTTCTGCCATTAAGGCAGCTGGAAAAGCTTGGTACAACACTATGGTCTCGGATTCAGATTATACAGAATTTGACAACTTCTCCAAGTGGTTGGGTGTTTCACAGTGA
Protein sequence

>Msa0110630

MASAAARPLVTVQTLDSDMATDSQTTLPIPDVMRASIRPDIVNFVHSNISKNSRQPYAVSRKAGHQTSAESWGTGRAVSRIPRVAGGGTHRAGQAAFGNMCRGGRMFAPTRIWRKWHRKINVNQKRYAVVSAIAASAIPSLVLARGHRIETVPEFPLVVGDSAEGVEKTKEAISVLKKIGAFADAEKAKASLGIRPGKGKMRNRRYISRKGPLIVYGTEGAKAVKAFRNIPGVEITNVERLNLLKLAPGGHLGRFVVWTKSAFEKLDSIYGTFDKGSEKKKGYVLPRAKMVNSDLTRIINSDEVQSVVRPIKKDVKRATLKKNPLKNLNVMLRLNPYAKTAKRMALLAEAERVKAKKEKLDKKRKTVSKEEASAIKAAGKAWYNTMVSDSDYTEFDNFSKWLGVSQ*