AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0116480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116480 MtrunA17_Chr1g0193061 98.519 135 2 0 2 136 92 226 4.66e-100 286
Msa0116480 MtrunA17_Chr8g0365921 73.643 129 33 1 2 129 88 216 8.30e-67 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0116480 AT1G56700.3 75.969 129 31 0 2 130 60 188 3.64e-76 224
Msa0116480 AT1G56700.1 75.969 129 31 0 2 130 89 217 2.09e-75 223
Msa0116480 AT1G56700.2 75.969 129 31 0 2 130 89 217 2.09e-75 223
Msa0116480 AT1G56700.4 75.969 129 31 0 2 130 89 217 2.09e-75 223
Msa0116480 AT1G23440.1 70.866 127 37 0 2 128 88 214 5.95e-67 202
Msa0116480 AT1G23440.3 70.866 127 37 0 2 128 88 214 5.95e-67 202

Find 56 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGTTCCAATAAAATTATC+TGG 0.221027 1_3:+35483178 Msa0116480:five_prime_UTR
ATGTTTGTTCAGCTGCATTT+TGG 0.221330 1_3:+35483564 Msa0116480:intron
TAGATATGATAATATAATTA+TGG 0.272196 1_3:+35483386 Msa0116480:five_prime_UTR
ATTTGCTGCTTCCTTATTAG+AGG 0.294466 1_3:+35484428 Msa0116480:CDS
ACCTCCCGTATTCTTGAAAT+CGG 0.310925 1_3:-35483797 None:intergenic
CTTCCGTTGCCCTGATGAAA+TGG 0.315906 1_3:+35483644 Msa0116480:CDS
AGGAAGCAGCAAATTGCATT+TGG 0.316795 1_3:-35484419 None:intergenic
ATTAGCAAGTACCTCTAATA+AGG 0.317751 1_3:-35484439 None:intergenic
GGCTTCCATCCCATTTCATC+AGG 0.344197 1_3:-35483654 None:intergenic
TTAGCATAGAACTGAAATAA+TGG 0.399329 1_3:+35483464 Msa0116480:exon
CTATCATTCTCTTCGGTTCG+CGG 0.401359 1_3:+35484332 Msa0116480:CDS
ACCTTTCGTTGCCAAGGTCT+TGG 0.402676 1_3:-35484256 None:intergenic
GGAAGCAGCAAATTGCATTT+GGG 0.414368 1_3:-35484418 None:intergenic
ATGTTTACTATCATTCTCTT+CGG 0.426824 1_3:+35484325 Msa0116480:CDS
ACCAGAACAATTACATGAGC+TGG 0.432033 1_3:-35484473 None:intergenic
CTTAGTCATACTTTGTGTGT+AGG 0.452475 1_3:+35483238 Msa0116480:five_prime_UTR
GTCTTGGTCATCTGCTCCAC+AGG 0.464244 1_3:-35484240 None:intergenic
ATGGCCCGATTTCAAGAATA+CGG 0.466039 1_3:+35483792 Msa0116480:CDS
GTTCCCATTGTTCCTTCAGA+TGG 0.480004 1_3:+35483773 Msa0116480:CDS
TACTCACAAAGTAGGATCCT+TGG 0.492422 1_3:-35483209 None:intergenic
CCTCCCGTATTCTTGAAATC+GGG 0.492780 1_3:-35483796 None:intergenic
ATCATAACCTTTCGTTGCCA+AGG 0.509665 1_3:-35484262 None:intergenic
TACATAAATAAAACTTCAGA+AGG 0.511970 1_3:-35483274 None:intergenic
TAACTGCGACTAAAGATCAC+TGG 0.518612 1_3:+35483412 Msa0116480:five_prime_UTR
ATCATATCTAAACAAGGATA+CGG 0.523644 1_3:-35483373 None:intergenic
AGGATCCTACTTTGTGAGTA+GGG 0.525838 1_3:+35483212 Msa0116480:five_prime_UTR
TTCCGTTGCCCTGATGAAAT+GGG 0.529742 1_3:+35483645 Msa0116480:CDS
CTACACACAAAGTATGACTA+AGG 0.530621 1_3:-35483237 None:intergenic
AAGTTCCAATAAAATTATCT+GGG 0.531862 1_3:+35483179 Msa0116480:five_prime_UTR
CGGGCCATCTGAAGGAACAA+TGG 0.539127 1_3:-35483777 None:intergenic
AAGGATCCTACTTTGTGAGT+AGG 0.540291 1_3:+35483211 Msa0116480:five_prime_UTR
GGGCCATCTGAAGGAACAAT+GGG 0.541004 1_3:-35483776 None:intergenic
ATTATCTGGGTGAGTTGCCA+AGG 0.544266 1_3:+35483192 Msa0116480:five_prime_UTR
TGGTCATCTGCTCCACAGGA+AGG 0.549415 1_3:-35484236 None:intergenic
TCATTTATTGTGAAGAAGAG+TGG 0.550686 1_3:-35484390 None:intergenic
CTTGAAATCGGGCCATCTGA+AGG 0.551552 1_3:-35483785 None:intergenic
ATCCCATTTCATCAGGGCAA+CGG 0.562088 1_3:-35483647 None:intergenic
ACCAAGACCTTGGCAACGAA+AGG 0.585242 1_3:+35484255 Msa0116480:CDS
GGAGCAGATGACCAAGACCT+TGG 0.592442 1_3:+35484245 Msa0116480:CDS
CTTCGGTTCGCGGAGCAGAA+CGG 0.596040 1_3:+35484342 Msa0116480:CDS
CCAGAACAATTACATGAGCT+GGG 0.598749 1_3:-35484472 None:intergenic
TGGCCCGATTTCAAGAATAC+GGG 0.601050 1_3:+35483793 Msa0116480:CDS
AGGCTCCCTACTCACAAAGT+AGG 0.607174 1_3:-35483217 None:intergenic
GGGTTAACAGTGGTGCAACA+AGG 0.614278 1_3:+35483586 Msa0116480:CDS
GGTCATCTGCTCCACAGGAA+GGG 0.614706 1_3:-35484235 None:intergenic
TGAAATGGGATGGAAGCCAC+AGG 0.624135 1_3:+35483659 Msa0116480:CDS
AAGATCACTGGAATTTGACA+TGG 0.627203 1_3:+35483424 Msa0116480:five_prime_UTR
CATTTCATCAGGGCAACGGA+AGG 0.630663 1_3:-35483643 None:intergenic
TATGCAGACTACCCTTCCTG+TGG 0.633375 1_3:+35484224 Msa0116480:intron
GCTTCCATCCCATTTCATCA+GGG 0.640372 1_3:-35483653 None:intergenic
GTTGCCCTGATGAAATGGGA+TGG 0.648441 1_3:+35483649 Msa0116480:CDS
CCCGATTTCAAGAATACGGG+AGG 0.650919 1_3:+35483796 Msa0116480:CDS
TGACATCTGATGATGCAGGA+AGG 0.659977 1_3:+35484289 Msa0116480:CDS
GTAATGACATCTGATGATGC+AGG 0.664436 1_3:+35484285 Msa0116480:CDS
TATATTATCATATCTAAACA+AGG 0.664563 1_3:-35483379 None:intergenic
AGAACGTCAAGCTGTCAATG+AGG 0.679006 1_3:+35483617 Msa0116480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGATATGATAATATAATTA+TGG + chr1_3:35483386-35483405 Msa0116480:five_prime_UTR 10.0%
!! TAGATATGATAATATAATTA+TGG + chr1_3:35483386-35483405 Msa0116480:five_prime_UTR 10.0%
!! TATATTATCATATCTAAACA+AGG - chr1_3:35483382-35483401 None:intergenic 15.0%
!! TATATTATCATATCTAAACA+AGG - chr1_3:35483382-35483401 None:intergenic 15.0%
!! AAGTTCCAATAAAATTATCT+GGG + chr1_3:35483179-35483198 Msa0116480:five_prime_UTR 20.0%
!! TACATAAATAAAACTTCAGA+AGG - chr1_3:35483277-35483296 None:intergenic 20.0%
!!! TTTCTGCAATAAAAGTTTTA+TGG - chr1_3:35483753-35483772 None:intergenic 20.0%
!! ATACAAATATGAAAATGTTG+AGG + chr1_3:35483881-35483900 Msa0116480:intron 20.0%
!! AAGTTCCAATAAAATTATCT+GGG + chr1_3:35483179-35483198 Msa0116480:five_prime_UTR 20.0%
!! TACATAAATAAAACTTCAGA+AGG - chr1_3:35483277-35483296 None:intergenic 20.0%
!!! TTTCTGCAATAAAAGTTTTA+TGG - chr1_3:35483753-35483772 None:intergenic 20.0%
!! ATACAAATATGAAAATGTTG+AGG + chr1_3:35483881-35483900 Msa0116480:intron 20.0%
! CAAGTTCCAATAAAATTATC+TGG + chr1_3:35483178-35483197 Msa0116480:five_prime_UTR 25.0%
! ATCATATCTAAACAAGGATA+CGG - chr1_3:35483376-35483395 None:intergenic 25.0%
! TTAGCATAGAACTGAAATAA+TGG + chr1_3:35483464-35483483 Msa0116480:exon 25.0%
! TTATCATGAATCTTATGTTC+TGG + chr1_3:35483539-35483558 Msa0116480:intron 25.0%
!! CTGTTCTTTAAATATTTCTG+AGG - chr1_3:35483935-35483954 None:intergenic 25.0%
! TTGTGAAATGATACTTAGAA+TGG - chr1_3:35483962-35483981 None:intergenic 25.0%
!!! AATTATTTTTTGCCTCTTGA+TGG + chr1_3:35484056-35484075 Msa0116480:intron 25.0%
! ATGTTTACTATCATTCTCTT+CGG + chr1_3:35484325-35484344 Msa0116480:CDS 25.0%
! CAAGTTCCAATAAAATTATC+TGG + chr1_3:35483178-35483197 Msa0116480:five_prime_UTR 25.0%
! ATCATATCTAAACAAGGATA+CGG - chr1_3:35483376-35483395 None:intergenic 25.0%
! TTAGCATAGAACTGAAATAA+TGG + chr1_3:35483464-35483483 Msa0116480:exon 25.0%
! TTATCATGAATCTTATGTTC+TGG + chr1_3:35483539-35483558 Msa0116480:intron 25.0%
!! CTGTTCTTTAAATATTTCTG+AGG - chr1_3:35483935-35483954 None:intergenic 25.0%
! TTGTGAAATGATACTTAGAA+TGG - chr1_3:35483962-35483981 None:intergenic 25.0%
!!! AATTATTTTTTGCCTCTTGA+TGG + chr1_3:35484056-35484075 Msa0116480:intron 25.0%
! ATGTTTACTATCATTCTCTT+CGG + chr1_3:35484325-35484344 Msa0116480:CDS 25.0%
! ACTCACCCAGATAATTTTAT+TGG - chr1_3:35483187-35483206 None:intergenic 30.0%
! AATGGTAAGAAAGTTCTAGT+TGG + chr1_3:35483482-35483501 Msa0116480:intron 30.0%
ATGAAAACAAGTTTAACCTG+TGG - chr1_3:35483678-35483697 None:intergenic 30.0%
! TTGTTTTCATCAACCCTTTT+AGG + chr1_3:35483688-35483707 Msa0116480:intron 30.0%
! TAATAGAGTACTTCAAGATC+AGG - chr1_3:35483714-35483733 None:intergenic 30.0%
! TTTTATGGAACTGTCACTAA+AGG - chr1_3:35483738-35483757 None:intergenic 30.0%
! TTCATTTTCAAAAACTTGCG+AGG + chr1_3:35484025-35484044 Msa0116480:intron 30.0%
AAAACATGGAACAGTCATTA+AGG - chr1_3:35484157-35484176 None:intergenic 30.0%
TTAGGACAAAAGACAAAGTT+AGG - chr1_3:35484201-35484220 None:intergenic 30.0%
TCATTTATTGTGAAGAAGAG+TGG - chr1_3:35484393-35484412 None:intergenic 30.0%
ATTAGCAAGTACCTCTAATA+AGG - chr1_3:35484442-35484461 None:intergenic 30.0%
! ACTCACCCAGATAATTTTAT+TGG - chr1_3:35483187-35483206 None:intergenic 30.0%
! AATGGTAAGAAAGTTCTAGT+TGG + chr1_3:35483482-35483501 Msa0116480:intron 30.0%
ATGAAAACAAGTTTAACCTG+TGG - chr1_3:35483678-35483697 None:intergenic 30.0%
! TTGTTTTCATCAACCCTTTT+AGG + chr1_3:35483688-35483707 Msa0116480:intron 30.0%
! TAATAGAGTACTTCAAGATC+AGG - chr1_3:35483714-35483733 None:intergenic 30.0%
! TTTTATGGAACTGTCACTAA+AGG - chr1_3:35483738-35483757 None:intergenic 30.0%
! TTCATTTTCAAAAACTTGCG+AGG + chr1_3:35484025-35484044 Msa0116480:intron 30.0%
AAAACATGGAACAGTCATTA+AGG - chr1_3:35484157-35484176 None:intergenic 30.0%
TTAGGACAAAAGACAAAGTT+AGG - chr1_3:35484201-35484220 None:intergenic 30.0%
TCATTTATTGTGAAGAAGAG+TGG - chr1_3:35484393-35484412 None:intergenic 30.0%
ATTAGCAAGTACCTCTAATA+AGG - chr1_3:35484442-35484461 None:intergenic 30.0%
CTACACACAAAGTATGACTA+AGG - chr1_3:35483240-35483259 None:intergenic 35.0%
CTTAGTCATACTTTGTGTGT+AGG + chr1_3:35483238-35483257 Msa0116480:five_prime_UTR 35.0%
AAGATCACTGGAATTTGACA+TGG + chr1_3:35483424-35483443 Msa0116480:five_prime_UTR 35.0%
! ATGTTTGTTCAGCTGCATTT+TGG + chr1_3:35483564-35483583 Msa0116480:intron 35.0%
! TGTTTGTTCAGCTGCATTTT+GGG + chr1_3:35483565-35483584 Msa0116480:intron 35.0%
! TTTTCGATTATCTGAGTCCT+TGG + chr1_3:35483984-35484003 Msa0116480:intron 35.0%
! CACAAAGTTTCTAGATACCA+AGG - chr1_3:35484004-35484023 None:intergenic 35.0%
!!! TATTTTTTGCCTCTTGATGG+AGG + chr1_3:35484059-35484078 Msa0116480:intron 35.0%
!!! TGTTCCATGTTTTTGAGATG+CGG + chr1_3:35484164-35484183 Msa0116480:intron 35.0%
! ATTTGCTGCTTCCTTATTAG+AGG + chr1_3:35484428-35484447 Msa0116480:CDS 35.0%
CTACACACAAAGTATGACTA+AGG - chr1_3:35483240-35483259 None:intergenic 35.0%
CTTAGTCATACTTTGTGTGT+AGG + chr1_3:35483238-35483257 Msa0116480:five_prime_UTR 35.0%
AAGATCACTGGAATTTGACA+TGG + chr1_3:35483424-35483443 Msa0116480:five_prime_UTR 35.0%
! ATGTTTGTTCAGCTGCATTT+TGG + chr1_3:35483564-35483583 Msa0116480:intron 35.0%
! TGTTTGTTCAGCTGCATTTT+GGG + chr1_3:35483565-35483584 Msa0116480:intron 35.0%
! TTTTCGATTATCTGAGTCCT+TGG + chr1_3:35483984-35484003 Msa0116480:intron 35.0%
! CACAAAGTTTCTAGATACCA+AGG - chr1_3:35484004-35484023 None:intergenic 35.0%
!!! TATTTTTTGCCTCTTGATGG+AGG + chr1_3:35484059-35484078 Msa0116480:intron 35.0%
!!! TGTTCCATGTTTTTGAGATG+CGG + chr1_3:35484164-35484183 Msa0116480:intron 35.0%
! ATTTGCTGCTTCCTTATTAG+AGG + chr1_3:35484428-35484447 Msa0116480:CDS 35.0%
TACTCACAAAGTAGGATCCT+TGG - chr1_3:35483212-35483231 None:intergenic 40.0%
AAGGATCCTACTTTGTGAGT+AGG + chr1_3:35483211-35483230 Msa0116480:five_prime_UTR 40.0%
AGGATCCTACTTTGTGAGTA+GGG + chr1_3:35483212-35483231 Msa0116480:five_prime_UTR 40.0%
TAACTGCGACTAAAGATCAC+TGG + chr1_3:35483412-35483431 Msa0116480:five_prime_UTR 40.0%
! GTTTGTTCAGCTGCATTTTG+GGG + chr1_3:35483566-35483585 Msa0116480:intron 40.0%
CTTCAAGATCAGGCCTAAAA+GGG - chr1_3:35483704-35483723 None:intergenic 40.0%
ACTTCAAGATCAGGCCTAAA+AGG - chr1_3:35483705-35483724 None:intergenic 40.0%
ATGGCCCGATTTCAAGAATA+CGG + chr1_3:35483792-35483811 Msa0116480:CDS 40.0%
ACCTCCCGTATTCTTGAAAT+CGG - chr1_3:35483800-35483819 None:intergenic 40.0%
CAAGAATACGGGAGGTAAAT+TGG + chr1_3:35483804-35483823 Msa0116480:intron 40.0%
AGGTGCAAGTGCAAAGATAT+TGG + chr1_3:35484088-35484107 Msa0116480:intron 40.0%
ACAACCGCATCTCAAAAACA+TGG - chr1_3:35484171-35484190 None:intergenic 40.0%
AGGGTAGTCTGCATAAAGTT+AGG - chr1_3:35484219-35484238 None:intergenic 40.0%
ATCATAACCTTTCGTTGCCA+AGG - chr1_3:35484265-35484284 None:intergenic 40.0%
! GTAATGACATCTGATGATGC+AGG + chr1_3:35484285-35484304 Msa0116480:CDS 40.0%
GGAAGCAGCAAATTGCATTT+GGG - chr1_3:35484421-35484440 None:intergenic 40.0%
AGGAAGCAGCAAATTGCATT+TGG - chr1_3:35484422-35484441 None:intergenic 40.0%
TACTCACAAAGTAGGATCCT+TGG - chr1_3:35483212-35483231 None:intergenic 40.0%
AAGGATCCTACTTTGTGAGT+AGG + chr1_3:35483211-35483230 Msa0116480:five_prime_UTR 40.0%
AGGATCCTACTTTGTGAGTA+GGG + chr1_3:35483212-35483231 Msa0116480:five_prime_UTR 40.0%
TAACTGCGACTAAAGATCAC+TGG + chr1_3:35483412-35483431 Msa0116480:five_prime_UTR 40.0%
! GTTTGTTCAGCTGCATTTTG+GGG + chr1_3:35483566-35483585 Msa0116480:intron 40.0%
CTTCAAGATCAGGCCTAAAA+GGG - chr1_3:35483704-35483723 None:intergenic 40.0%
ACTTCAAGATCAGGCCTAAA+AGG - chr1_3:35483705-35483724 None:intergenic 40.0%
ATGGCCCGATTTCAAGAATA+CGG + chr1_3:35483792-35483811 Msa0116480:CDS 40.0%
ACCTCCCGTATTCTTGAAAT+CGG - chr1_3:35483800-35483819 None:intergenic 40.0%
CAAGAATACGGGAGGTAAAT+TGG + chr1_3:35483804-35483823 Msa0116480:intron 40.0%
AGGTGCAAGTGCAAAGATAT+TGG + chr1_3:35484088-35484107 Msa0116480:intron 40.0%
ACAACCGCATCTCAAAAACA+TGG - chr1_3:35484171-35484190 None:intergenic 40.0%
AGGGTAGTCTGCATAAAGTT+AGG - chr1_3:35484219-35484238 None:intergenic 40.0%
ATCATAACCTTTCGTTGCCA+AGG - chr1_3:35484265-35484284 None:intergenic 40.0%
! GTAATGACATCTGATGATGC+AGG + chr1_3:35484285-35484304 Msa0116480:CDS 40.0%
GGAAGCAGCAAATTGCATTT+GGG - chr1_3:35484421-35484440 None:intergenic 40.0%
AGGAAGCAGCAAATTGCATT+TGG - chr1_3:35484422-35484441 None:intergenic 40.0%
ATTATCTGGGTGAGTTGCCA+AGG + chr1_3:35483192-35483211 Msa0116480:five_prime_UTR 45.0%
!!! CTGCATTTTGGGGTTAACAG+TGG + chr1_3:35483576-35483595 Msa0116480:CDS 45.0%
AGAACGTCAAGCTGTCAATG+AGG + chr1_3:35483617-35483636 Msa0116480:CDS 45.0%
TTCCGTTGCCCTGATGAAAT+GGG + chr1_3:35483645-35483664 Msa0116480:CDS 45.0%
ATCCCATTTCATCAGGGCAA+CGG - chr1_3:35483650-35483669 None:intergenic 45.0%
GCTTCCATCCCATTTCATCA+GGG - chr1_3:35483656-35483675 None:intergenic 45.0%
GTTCCCATTGTTCCTTCAGA+TGG + chr1_3:35483773-35483792 Msa0116480:CDS 45.0%
TGGCCCGATTTCAAGAATAC+GGG + chr1_3:35483793-35483812 Msa0116480:CDS 45.0%
CCTCCCGTATTCTTGAAATC+GGG - chr1_3:35483799-35483818 None:intergenic 45.0%
CCTTATATGCCTCCATCAAG+AGG - chr1_3:35484071-35484090 None:intergenic 45.0%
! CCTCTTGATGGAGGCATATA+AGG + chr1_3:35484068-35484087 Msa0116480:intron 45.0%
! TGACATCTGATGATGCAGGA+AGG + chr1_3:35484289-35484308 Msa0116480:CDS 45.0%
! CTATCATTCTCTTCGGTTCG+CGG + chr1_3:35484332-35484351 Msa0116480:CDS 45.0%
ATTATCTGGGTGAGTTGCCA+AGG + chr1_3:35483192-35483211 Msa0116480:five_prime_UTR 45.0%
!!! CTGCATTTTGGGGTTAACAG+TGG + chr1_3:35483576-35483595 Msa0116480:CDS 45.0%
AGAACGTCAAGCTGTCAATG+AGG + chr1_3:35483617-35483636 Msa0116480:CDS 45.0%
TTCCGTTGCCCTGATGAAAT+GGG + chr1_3:35483645-35483664 Msa0116480:CDS 45.0%
ATCCCATTTCATCAGGGCAA+CGG - chr1_3:35483650-35483669 None:intergenic 45.0%
GCTTCCATCCCATTTCATCA+GGG - chr1_3:35483656-35483675 None:intergenic 45.0%
GTTCCCATTGTTCCTTCAGA+TGG + chr1_3:35483773-35483792 Msa0116480:CDS 45.0%
TGGCCCGATTTCAAGAATAC+GGG + chr1_3:35483793-35483812 Msa0116480:CDS 45.0%
CCTCCCGTATTCTTGAAATC+GGG - chr1_3:35483799-35483818 None:intergenic 45.0%
CCTTATATGCCTCCATCAAG+AGG - chr1_3:35484071-35484090 None:intergenic 45.0%
! CCTCTTGATGGAGGCATATA+AGG + chr1_3:35484068-35484087 Msa0116480:intron 45.0%
! TGACATCTGATGATGCAGGA+AGG + chr1_3:35484289-35484308 Msa0116480:CDS 45.0%
! CTATCATTCTCTTCGGTTCG+CGG + chr1_3:35484332-35484351 Msa0116480:CDS 45.0%
!!! ATTTTATAATATCATTTATT+TGG + chr1_3:35483907-35483926 Msa0116480:intron 5.0%
!!! ATTTTATAATATCATTTATT+TGG + chr1_3:35483907-35483926 Msa0116480:intron 5.0%
AGGCTCCCTACTCACAAAGT+AGG - chr1_3:35483220-35483239 None:intergenic 50.0%
GGGTTAACAGTGGTGCAACA+AGG + chr1_3:35483586-35483605 Msa0116480:CDS 50.0%
CATTTCATCAGGGCAACGGA+AGG - chr1_3:35483646-35483665 None:intergenic 50.0%
CTTCCGTTGCCCTGATGAAA+TGG + chr1_3:35483644-35483663 Msa0116480:CDS 50.0%
GTTGCCCTGATGAAATGGGA+TGG + chr1_3:35483649-35483668 Msa0116480:CDS 50.0%
GGCTTCCATCCCATTTCATC+AGG - chr1_3:35483657-35483676 None:intergenic 50.0%
TGAAATGGGATGGAAGCCAC+AGG + chr1_3:35483659-35483678 Msa0116480:CDS 50.0%
GGGCCATCTGAAGGAACAAT+GGG - chr1_3:35483779-35483798 None:intergenic 50.0%
CTTGAAATCGGGCCATCTGA+AGG - chr1_3:35483788-35483807 None:intergenic 50.0%
CCCGATTTCAAGAATACGGG+AGG + chr1_3:35483796-35483815 Msa0116480:CDS 50.0%
TATGCAGACTACCCTTCCTG+TGG + chr1_3:35484224-35484243 Msa0116480:intron 50.0%
ACCAAGACCTTGGCAACGAA+AGG + chr1_3:35484255-35484274 Msa0116480:CDS 50.0%
ACCTTTCGTTGCCAAGGTCT+TGG - chr1_3:35484259-35484278 None:intergenic 50.0%
AGGCTCCCTACTCACAAAGT+AGG - chr1_3:35483220-35483239 None:intergenic 50.0%
GGGTTAACAGTGGTGCAACA+AGG + chr1_3:35483586-35483605 Msa0116480:CDS 50.0%
CATTTCATCAGGGCAACGGA+AGG - chr1_3:35483646-35483665 None:intergenic 50.0%
CTTCCGTTGCCCTGATGAAA+TGG + chr1_3:35483644-35483663 Msa0116480:CDS 50.0%
GTTGCCCTGATGAAATGGGA+TGG + chr1_3:35483649-35483668 Msa0116480:CDS 50.0%
GGCTTCCATCCCATTTCATC+AGG - chr1_3:35483657-35483676 None:intergenic 50.0%
TGAAATGGGATGGAAGCCAC+AGG + chr1_3:35483659-35483678 Msa0116480:CDS 50.0%
GGGCCATCTGAAGGAACAAT+GGG - chr1_3:35483779-35483798 None:intergenic 50.0%
CTTGAAATCGGGCCATCTGA+AGG - chr1_3:35483788-35483807 None:intergenic 50.0%
CCCGATTTCAAGAATACGGG+AGG + chr1_3:35483796-35483815 Msa0116480:CDS 50.0%
TATGCAGACTACCCTTCCTG+TGG + chr1_3:35484224-35484243 Msa0116480:intron 50.0%
ACCAAGACCTTGGCAACGAA+AGG + chr1_3:35484255-35484274 Msa0116480:CDS 50.0%
ACCTTTCGTTGCCAAGGTCT+TGG - chr1_3:35484259-35484278 None:intergenic 50.0%
CGGGCCATCTGAAGGAACAA+TGG - chr1_3:35483780-35483799 None:intergenic 55.0%
CGGGAGGTAAATTGGATCGC+TGG + chr1_3:35483812-35483831 Msa0116480:intron 55.0%
GGTCATCTGCTCCACAGGAA+GGG - chr1_3:35484238-35484257 None:intergenic 55.0%
TGGTCATCTGCTCCACAGGA+AGG - chr1_3:35484239-35484258 None:intergenic 55.0%
GTCTTGGTCATCTGCTCCAC+AGG - chr1_3:35484243-35484262 None:intergenic 55.0%
GGAGCAGATGACCAAGACCT+TGG + chr1_3:35484245-35484264 Msa0116480:CDS 55.0%
CGGGCCATCTGAAGGAACAA+TGG - chr1_3:35483780-35483799 None:intergenic 55.0%
CGGGAGGTAAATTGGATCGC+TGG + chr1_3:35483812-35483831 Msa0116480:intron 55.0%
GGTCATCTGCTCCACAGGAA+GGG - chr1_3:35484238-35484257 None:intergenic 55.0%
TGGTCATCTGCTCCACAGGA+AGG - chr1_3:35484239-35484258 None:intergenic 55.0%
GTCTTGGTCATCTGCTCCAC+AGG - chr1_3:35484243-35484262 None:intergenic 55.0%
GGAGCAGATGACCAAGACCT+TGG + chr1_3:35484245-35484264 Msa0116480:CDS 55.0%
! CTTCGGTTCGCGGAGCAGAA+CGG + chr1_3:35484342-35484361 Msa0116480:CDS 60.0%
! CTTCGGTTCGCGGAGCAGAA+CGG + chr1_3:35484342-35484361 Msa0116480:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 35483177 35484485 35483177 ID=Msa0116480;Name=Msa0116480
chr1_3 mRNA 35483177 35484485 35483177 ID=Msa0116480-mRNA-1;Parent=Msa0116480;Name=Msa0116480-mRNA-1;_AED=0.04;_eAED=0.04;_QI=306|0.33|0.25|1|1|1|4|0|136
chr1_3 exon 35483177 35483485 35483177 ID=Msa0116480-mRNA-1:exon:11322;Parent=Msa0116480-mRNA-1
chr1_3 exon 35483576 35483680 35483576 ID=Msa0116480-mRNA-1:exon:11323;Parent=Msa0116480-mRNA-1
chr1_3 exon 35483770 35483817 35483770 ID=Msa0116480-mRNA-1:exon:11324;Parent=Msa0116480-mRNA-1
chr1_3 exon 35484231 35484485 35484231 ID=Msa0116480-mRNA-1:exon:11325;Parent=Msa0116480-mRNA-1
chr1_3 five_prime_UTR 35483177 35483482 35483177 ID=Msa0116480-mRNA-1:five_prime_utr;Parent=Msa0116480-mRNA-1
chr1_3 CDS 35483483 35483485 35483483 ID=Msa0116480-mRNA-1:cds;Parent=Msa0116480-mRNA-1
chr1_3 CDS 35483576 35483680 35483576 ID=Msa0116480-mRNA-1:cds;Parent=Msa0116480-mRNA-1
chr1_3 CDS 35483770 35483817 35483770 ID=Msa0116480-mRNA-1:cds;Parent=Msa0116480-mRNA-1
chr1_3 CDS 35484231 35484485 35484231 ID=Msa0116480-mRNA-1:cds;Parent=Msa0116480-mRNA-1
Gene Sequence

>Msa0116480

ATGCTGCATTTTGGGGTTAACAGTGGTGCAACAAGGTTCGCTATAGAACGTCAAGCTGTCAATGAGGCTACCTTCCGTTGCCCTGATGAAATGGGATGGAAGCCACAGAAAGTTCCCATTGTTCCTTCAGATGGCCCGATTTCAAGAATACGGGAGACTACCCTTCCTGTGGAGCAGATGACCAAGACCTTGGCAACGAAAGGTTATGATGTAATGACATCTGATGATGCAGGAAGGTTTGTGTGCAATTATGTTTACTATCATTCTCTTCGGTTCGCGGAGCAGAACGGAAACAAATCACTTTTCGTGCATGTGCCACTCTTCTTCACAATAAATGAAGAAACCCAAATGCAATTTGCTGCTTCCTTATTAGAGGTACTTGCTAATGTTAGTAACACCCAGCTCATGTAA
Protein sequence

>Msa0116480

MLHFGVNSGATRFAIERQAVNEATFRCPDEMGWKPQKVPIVPSDGPISRIRETTLPVEQMTKTLATKGYDVMTSDDAGRFVCNYVYYHSLRFAEQNGNKSLFVHVPLFFTINEETQMQFAASLLEVLANVSNTQLM*