AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0291880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0291880 MtrunA17_Chr2g0312011 100.000 58 0 0 1 58 1 58 9.97e-39 124
Msa0291880 MtrunA17_Chr2g0312051 57.377 61 22 1 1 57 1 61 1.96e-15 65.9
Msa0291880 MtrunA17_Chr4g0048281 57.377 61 19 3 4 57 5 65 1.17e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTAGCAGGGGTGATGGTTTC+TGG 0.154253 2_3:+40613935 Msa0291880:CDS
AATATTTGTGCTTGTCTTCA+TGG 0.210203 2_3:+40614470 Msa0291880:CDS
TAAAGATCCATGTTACAAAT+TGG 0.278218 2_3:-40614574 None:intergenic
AGGATACAAAGATTCTTATT+AGG 0.301506 2_3:-40614530 None:intergenic
TCCAAGTGTGTGTGATTAAC+TGG 0.369868 2_3:-40613498 None:intergenic
TTTAATTGGAACACTTTGTT+GGG 0.371717 2_3:-40613908 None:intergenic
ATAAGAATCTTTGTATCCTA+TGG 0.390499 2_3:+40614534 Msa0291880:CDS
AGCTGTTCCACCACCACCTA+TGG 0.399384 2_3:+40613864 Msa0291880:CDS
ATTATGTTGTTGCTGTGCCC+TGG 0.400888 2_3:+40614406 Msa0291880:CDS
ATATTGTGACTCATTGTGTT+AGG 0.426555 2_3:-40613583 None:intergenic
ACTTTGTTGGGGATCATCCT+TGG 0.445500 2_3:-40613896 None:intergenic
CTGATCGAATATCATACAAC+TGG 0.456561 2_3:+40614439 Msa0291880:CDS
TCGATCAGAGGCAACAATCC+AGG 0.459282 2_3:-40614424 None:intergenic
TTGTTGGGGATCATCCTTGG+AGG 0.481114 2_3:-40613893 None:intergenic
GGGTTGTTCTGACTCACTGC+TGG 0.483166 2_3:-40613838 None:intergenic
TTCCAATTAAAACAACTAGC+AGG 0.486226 2_3:+40613920 Msa0291880:CDS
GGATAACCCATAGGTGGTGG+TGG 0.506331 2_3:-40613871 None:intergenic
AAAACAACTAGCAGGGGTGA+TGG 0.528737 2_3:+40613928 Msa0291880:CDS
ATCAATCAACAACAATCTCC+TGG 0.529469 2_3:+40613604 Msa0291880:CDS
TAAGAATCTTTGTATCCTAT+GGG 0.536702 2_3:+40614535 Msa0291880:CDS
GGTGGTGGTGGAACAGCTTG+AGG 0.543912 2_3:-40613859 None:intergenic
CGATCAGAGGCAACAATCCA+GGG 0.546345 2_3:-40614423 None:intergenic
TCCAGTTAATCACACACACT+TGG 0.546579 2_3:+40613497 Msa0291880:five_prime_UTR
GTGGTGGTGGAACAGCTTGA+GGG 0.555087 2_3:-40613858 None:intergenic
GAGGGATAACCCATAGGTGG+TGG 0.556470 2_3:-40613874 None:intergenic
CAAAGATTCTTATTAGGTGA+GGG 0.558800 2_3:-40614524 None:intergenic
TCCAATTAAAACAACTAGCA+GGG 0.562056 2_3:+40613921 Msa0291880:CDS
ACAAAGATTCTTATTAGGTG+AGG 0.569641 2_3:-40614525 None:intergenic
AGGGGTGATGGTTTCTGGAA+AGG 0.573043 2_3:+40613940 Msa0291880:CDS
ATTCATACCAATTTGTAACA+TGG 0.575973 2_3:+40614567 Msa0291880:exon
GCTGTTCCACCACCACCTAT+GGG 0.592890 2_3:+40613865 Msa0291880:CDS
ACCTATGGGTTATCCCTCCA+AGG 0.593969 2_3:+40613879 Msa0291880:CDS
GTGATGGTTTCTGGAAAGGA+TGG 0.594474 2_3:+40613944 Msa0291880:CDS
GTTGTATGATATTCGATCAG+AGG 0.597281 2_3:-40614436 None:intergenic
TGTTGGGGATCATCCTTGGA+GGG 0.597882 2_3:-40613892 None:intergenic
TTAATTGGAACACTTTGTTG+GGG 0.602125 2_3:-40613907 None:intergenic
CCAATTAAAACAACTAGCAG+GGG 0.643519 2_3:+40613922 Msa0291880:CDS
TCCTTGGAGGGATAACCCAT+AGG 0.667495 2_3:-40613880 None:intergenic
TAACTGGACGATGAGAAGAA+CGG 0.674180 2_3:-40613482 None:intergenic
TTGGAGGGATAACCCATAGG+TGG 0.675232 2_3:-40613877 None:intergenic
TTGCAACGACACAAGTACAA+AGG 0.678074 2_3:-40614601 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGTTATAGTTATTAATT+AGG - chr2_3:40613988-40614007 None:intergenic 10.0%
!! TATAGTTATAGTTATTAATT+AGG - chr2_3:40613988-40614007 None:intergenic 10.0%
!! ATAATTAAAAAAAACTTACC+AGG - chr2_3:40613625-40613644 None:intergenic 15.0%
!! CATTTCTATAAGAAATAATT+TGG + chr2_3:40613761-40613780 Msa0291880:intron 15.0%
!! AACTGCAAAAAAAAAAAAAA+AGG - chr2_3:40614379-40614398 None:intergenic 15.0%
!! ATAATTAAAAAAAACTTACC+AGG - chr2_3:40613625-40613644 None:intergenic 15.0%
!! CATTTCTATAAGAAATAATT+TGG + chr2_3:40613761-40613780 Msa0291880:intron 15.0%
!! AACTGCAAAAAAAAAAAAAA+AGG - chr2_3:40614379-40614398 None:intergenic 15.0%
!! CAAAAAAATTAAACGATTCT+AGG - chr2_3:40613727-40613746 None:intergenic 20.0%
!!! GCGATAACTTTTTTTTTTAA+TGG - chr2_3:40614190-40614209 None:intergenic 20.0%
!! TATTTATGCTAAAAACAGTT+TGG - chr2_3:40614336-40614355 None:intergenic 20.0%
!! CAAAAAAATTAAACGATTCT+AGG - chr2_3:40613727-40613746 None:intergenic 20.0%
!!! GCGATAACTTTTTTTTTTAA+TGG - chr2_3:40614190-40614209 None:intergenic 20.0%
!! TATTTATGCTAAAAACAGTT+TGG - chr2_3:40614336-40614355 None:intergenic 20.0%
! TTTAATTGGAACACTTTGTT+GGG - chr2_3:40613911-40613930 None:intergenic 25.0%
!!! TTTTAATTGGAACACTTTGT+TGG - chr2_3:40613912-40613931 None:intergenic 25.0%
!! TAACTTCTTTTCCTTTAACT+CGG - chr2_3:40614100-40614119 None:intergenic 25.0%
!!! AGTTTTTTTCTCCTTTGTTA+GGG - chr2_3:40614140-40614159 None:intergenic 25.0%
!!! TAGTTTTTTTCTCCTTTGTT+AGG - chr2_3:40614141-40614160 None:intergenic 25.0%
! AGGATACAAAGATTCTTATT+AGG - chr2_3:40614533-40614552 None:intergenic 25.0%
! ATAAGAATCTTTGTATCCTA+TGG + chr2_3:40614534-40614553 Msa0291880:CDS 25.0%
! TAAGAATCTTTGTATCCTAT+GGG + chr2_3:40614535-40614554 Msa0291880:CDS 25.0%
! ATTCATACCAATTTGTAACA+TGG + chr2_3:40614567-40614586 Msa0291880:exon 25.0%
! TAAAGATCCATGTTACAAAT+TGG - chr2_3:40614577-40614596 None:intergenic 25.0%
! TTTAATTGGAACACTTTGTT+GGG - chr2_3:40613911-40613930 None:intergenic 25.0%
!!! TTTTAATTGGAACACTTTGT+TGG - chr2_3:40613912-40613931 None:intergenic 25.0%
!! TAACTTCTTTTCCTTTAACT+CGG - chr2_3:40614100-40614119 None:intergenic 25.0%
!!! AGTTTTTTTCTCCTTTGTTA+GGG - chr2_3:40614140-40614159 None:intergenic 25.0%
!!! TAGTTTTTTTCTCCTTTGTT+AGG - chr2_3:40614141-40614160 None:intergenic 25.0%
! AGGATACAAAGATTCTTATT+AGG - chr2_3:40614533-40614552 None:intergenic 25.0%
! ATAAGAATCTTTGTATCCTA+TGG + chr2_3:40614534-40614553 Msa0291880:CDS 25.0%
! TAAGAATCTTTGTATCCTAT+GGG + chr2_3:40614535-40614554 Msa0291880:CDS 25.0%
! ATTCATACCAATTTGTAACA+TGG + chr2_3:40614567-40614586 Msa0291880:exon 25.0%
! TAAAGATCCATGTTACAAAT+TGG - chr2_3:40614577-40614596 None:intergenic 25.0%
ATATTGTGACTCATTGTGTT+AGG - chr2_3:40613586-40613605 None:intergenic 30.0%
CTAGGCTTGAATATAAAACA+TGG - chr2_3:40613709-40613728 None:intergenic 30.0%
TTAATTGGAACACTTTGTTG+GGG - chr2_3:40613910-40613929 None:intergenic 30.0%
TTCCAATTAAAACAACTAGC+AGG + chr2_3:40613920-40613939 Msa0291880:CDS 30.0%
TCCAATTAAAACAACTAGCA+GGG + chr2_3:40613921-40613940 Msa0291880:CDS 30.0%
GAAATTGAAAACCGAGTTAA+AGG + chr2_3:40614086-40614105 Msa0291880:intron 30.0%
GTTAAAGGAAAAGAAGTTAG+AGG + chr2_3:40614101-40614120 Msa0291880:intron 30.0%
!!! TTTTTTTTTTGCAGTTGTGC+TGG + chr2_3:40614383-40614402 Msa0291880:intron 30.0%
AATATTTGTGCTTGTCTTCA+TGG + chr2_3:40614470-40614489 Msa0291880:CDS 30.0%
CAAAGATTCTTATTAGGTGA+GGG - chr2_3:40614527-40614546 None:intergenic 30.0%
ACAAAGATTCTTATTAGGTG+AGG - chr2_3:40614528-40614547 None:intergenic 30.0%
ATATTGTGACTCATTGTGTT+AGG - chr2_3:40613586-40613605 None:intergenic 30.0%
CTAGGCTTGAATATAAAACA+TGG - chr2_3:40613709-40613728 None:intergenic 30.0%
TTAATTGGAACACTTTGTTG+GGG - chr2_3:40613910-40613929 None:intergenic 30.0%
TTCCAATTAAAACAACTAGC+AGG + chr2_3:40613920-40613939 Msa0291880:CDS 30.0%
TCCAATTAAAACAACTAGCA+GGG + chr2_3:40613921-40613940 Msa0291880:CDS 30.0%
GAAATTGAAAACCGAGTTAA+AGG + chr2_3:40614086-40614105 Msa0291880:intron 30.0%
GTTAAAGGAAAAGAAGTTAG+AGG + chr2_3:40614101-40614120 Msa0291880:intron 30.0%
!!! TTTTTTTTTTGCAGTTGTGC+TGG + chr2_3:40614383-40614402 Msa0291880:intron 30.0%
AATATTTGTGCTTGTCTTCA+TGG + chr2_3:40614470-40614489 Msa0291880:CDS 30.0%
CAAAGATTCTTATTAGGTGA+GGG - chr2_3:40614527-40614546 None:intergenic 30.0%
ACAAAGATTCTTATTAGGTG+AGG - chr2_3:40614528-40614547 None:intergenic 30.0%
ATCAATCAACAACAATCTCC+TGG + chr2_3:40613604-40613623 Msa0291880:CDS 35.0%
CGAAATACACAACACAAGTT+AGG - chr2_3:40613799-40613818 None:intergenic 35.0%
CCAATTAAAACAACTAGCAG+GGG + chr2_3:40613922-40613941 Msa0291880:CDS 35.0%
!! GAAAAGAAGTTAGAGGTGTT+AGG + chr2_3:40614108-40614127 Msa0291880:intron 35.0%
TTAGGTTCAAACCCTAACAA+AGG + chr2_3:40614126-40614145 Msa0291880:intron 35.0%
GTTGTATGATATTCGATCAG+AGG - chr2_3:40614439-40614458 None:intergenic 35.0%
CTGATCGAATATCATACAAC+TGG + chr2_3:40614439-40614458 Msa0291880:CDS 35.0%
ATCAATCAACAACAATCTCC+TGG + chr2_3:40613604-40613623 Msa0291880:CDS 35.0%
CGAAATACACAACACAAGTT+AGG - chr2_3:40613799-40613818 None:intergenic 35.0%
CCAATTAAAACAACTAGCAG+GGG + chr2_3:40613922-40613941 Msa0291880:CDS 35.0%
!! GAAAAGAAGTTAGAGGTGTT+AGG + chr2_3:40614108-40614127 Msa0291880:intron 35.0%
TTAGGTTCAAACCCTAACAA+AGG + chr2_3:40614126-40614145 Msa0291880:intron 35.0%
GTTGTATGATATTCGATCAG+AGG - chr2_3:40614439-40614458 None:intergenic 35.0%
CTGATCGAATATCATACAAC+TGG + chr2_3:40614439-40614458 Msa0291880:CDS 35.0%
TCCAGTTAATCACACACACT+TGG + chr2_3:40613497-40613516 Msa0291880:five_prime_UTR 40.0%
TCCAAGTGTGTGTGATTAAC+TGG - chr2_3:40613501-40613520 None:intergenic 40.0%
!!! CCCCTGCTAGTTGTTTTAAT+TGG - chr2_3:40613925-40613944 None:intergenic 40.0%
! ATGAATTTTCGCTGTCCCAT+AGG - chr2_3:40614553-40614572 None:intergenic 40.0%
TTGCAACGACACAAGTACAA+AGG - chr2_3:40614604-40614623 None:intergenic 40.0%
TCCAGTTAATCACACACACT+TGG + chr2_3:40613497-40613516 Msa0291880:five_prime_UTR 40.0%
TCCAAGTGTGTGTGATTAAC+TGG - chr2_3:40613501-40613520 None:intergenic 40.0%
!!! CCCCTGCTAGTTGTTTTAAT+TGG - chr2_3:40613925-40613944 None:intergenic 40.0%
! ATGAATTTTCGCTGTCCCAT+AGG - chr2_3:40614553-40614572 None:intergenic 40.0%
TTGCAACGACACAAGTACAA+AGG - chr2_3:40614604-40614623 None:intergenic 40.0%
ACTTTGTTGGGGATCATCCT+TGG - chr2_3:40613899-40613918 None:intergenic 45.0%
AAAACAACTAGCAGGGGTGA+TGG + chr2_3:40613928-40613947 Msa0291880:CDS 45.0%
! GTGATGGTTTCTGGAAAGGA+TGG + chr2_3:40613944-40613963 Msa0291880:CDS 45.0%
ATTATGTTGTTGCTGTGCCC+TGG + chr2_3:40614406-40614425 Msa0291880:CDS 45.0%
ACTTTGTTGGGGATCATCCT+TGG - chr2_3:40613899-40613918 None:intergenic 45.0%
AAAACAACTAGCAGGGGTGA+TGG + chr2_3:40613928-40613947 Msa0291880:CDS 45.0%
! GTGATGGTTTCTGGAAAGGA+TGG + chr2_3:40613944-40613963 Msa0291880:CDS 45.0%
ATTATGTTGTTGCTGTGCCC+TGG + chr2_3:40614406-40614425 Msa0291880:CDS 45.0%
TTGGAGGGATAACCCATAGG+TGG - chr2_3:40613880-40613899 None:intergenic 50.0%
! ACCTATGGGTTATCCCTCCA+AGG + chr2_3:40613879-40613898 Msa0291880:CDS 50.0%
TCCTTGGAGGGATAACCCAT+AGG - chr2_3:40613883-40613902 None:intergenic 50.0%
TGTTGGGGATCATCCTTGGA+GGG - chr2_3:40613895-40613914 None:intergenic 50.0%
TTGTTGGGGATCATCCTTGG+AGG - chr2_3:40613896-40613915 None:intergenic 50.0%
! AGGGGTGATGGTTTCTGGAA+AGG + chr2_3:40613940-40613959 Msa0291880:CDS 50.0%
CGATCAGAGGCAACAATCCA+GGG - chr2_3:40614426-40614445 None:intergenic 50.0%
TCGATCAGAGGCAACAATCC+AGG - chr2_3:40614427-40614446 None:intergenic 50.0%
TTGGAGGGATAACCCATAGG+TGG - chr2_3:40613880-40613899 None:intergenic 50.0%
! ACCTATGGGTTATCCCTCCA+AGG + chr2_3:40613879-40613898 Msa0291880:CDS 50.0%
TCCTTGGAGGGATAACCCAT+AGG - chr2_3:40613883-40613902 None:intergenic 50.0%
TGTTGGGGATCATCCTTGGA+GGG - chr2_3:40613895-40613914 None:intergenic 50.0%
TTGTTGGGGATCATCCTTGG+AGG - chr2_3:40613896-40613915 None:intergenic 50.0%
! AGGGGTGATGGTTTCTGGAA+AGG + chr2_3:40613940-40613959 Msa0291880:CDS 50.0%
CGATCAGAGGCAACAATCCA+GGG - chr2_3:40614426-40614445 None:intergenic 50.0%
TCGATCAGAGGCAACAATCC+AGG - chr2_3:40614427-40614446 None:intergenic 50.0%
GGGTTGTTCTGACTCACTGC+TGG - chr2_3:40613841-40613860 None:intergenic 55.0%
!! GTGGTGGTGGAACAGCTTGA+GGG - chr2_3:40613861-40613880 None:intergenic 55.0%
AGCTGTTCCACCACCACCTA+TGG + chr2_3:40613864-40613883 Msa0291880:CDS 55.0%
GCTGTTCCACCACCACCTAT+GGG + chr2_3:40613865-40613884 Msa0291880:CDS 55.0%
! GGATAACCCATAGGTGGTGG+TGG - chr2_3:40613874-40613893 None:intergenic 55.0%
GAGGGATAACCCATAGGTGG+TGG - chr2_3:40613877-40613896 None:intergenic 55.0%
! CTAGCAGGGGTGATGGTTTC+TGG + chr2_3:40613935-40613954 Msa0291880:CDS 55.0%
GGGTTGTTCTGACTCACTGC+TGG - chr2_3:40613841-40613860 None:intergenic 55.0%
!! GTGGTGGTGGAACAGCTTGA+GGG - chr2_3:40613861-40613880 None:intergenic 55.0%
AGCTGTTCCACCACCACCTA+TGG + chr2_3:40613864-40613883 Msa0291880:CDS 55.0%
GCTGTTCCACCACCACCTAT+GGG + chr2_3:40613865-40613884 Msa0291880:CDS 55.0%
! GGATAACCCATAGGTGGTGG+TGG - chr2_3:40613874-40613893 None:intergenic 55.0%
GAGGGATAACCCATAGGTGG+TGG - chr2_3:40613877-40613896 None:intergenic 55.0%
! CTAGCAGGGGTGATGGTTTC+TGG + chr2_3:40613935-40613954 Msa0291880:CDS 55.0%
!! GGTGGTGGTGGAACAGCTTG+AGG - chr2_3:40613862-40613881 None:intergenic 60.0%
!! GGTGGTGGTGGAACAGCTTG+AGG - chr2_3:40613862-40613881 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 40613484 40614746 40613484 ID=Msa0291880;Name=Msa0291880
chr2_3 mRNA 40613484 40614746 40613484 ID=Msa0291880-mRNA-1;Parent=Msa0291880;Name=Msa0291880-mRNA-1;_AED=0.01;_eAED=0.01;_QI=108|0.33|0.75|0.75|0.33|0.5|4|162|122
chr2_3 exon 40613484 40613625 40613484 ID=Msa0291880-mRNA-1:exon:13051;Parent=Msa0291880-mRNA-1
chr2_3 exon 40613827 40613965 40613827 ID=Msa0291880-mRNA-1:exon:13052;Parent=Msa0291880-mRNA-1
chr2_3 exon 40614255 40614262 40614255 ID=Msa0291880-mRNA-1:exon:13053;Parent=Msa0291880-mRNA-1
chr2_3 exon 40614397 40614746 40614397 ID=Msa0291880-mRNA-1:exon:13054;Parent=Msa0291880-mRNA-1
chr2_3 five_prime_UTR 40613484 40613591 40613484 ID=Msa0291880-mRNA-1:five_prime_utr;Parent=Msa0291880-mRNA-1
chr2_3 CDS 40613592 40613625 40613592 ID=Msa0291880-mRNA-1:cds;Parent=Msa0291880-mRNA-1
chr2_3 CDS 40613827 40613965 40613827 ID=Msa0291880-mRNA-1:cds;Parent=Msa0291880-mRNA-1
chr2_3 CDS 40614255 40614262 40614255 ID=Msa0291880-mRNA-1:cds;Parent=Msa0291880-mRNA-1
chr2_3 CDS 40614397 40614584 40614397 ID=Msa0291880-mRNA-1:cds;Parent=Msa0291880-mRNA-1
chr2_3 three_prime_UTR 40614585 40614746 40614585 ID=Msa0291880-mRNA-1:three_prime_utr;Parent=Msa0291880-mRNA-1
Gene Sequence

>Msa0291880

ATGAGTCACAATATCAATCAACAACAATCTCCTGTTACTGCATATCCAGCAGTGAGTCAGAACAACCCTCAAGCTGTTCCACCACCACCTATGGGTTATCCCTCCAAGGATGATCCCCAACAAAGTGTTCCAATTAAAACAACTAGCAGGGGTGATGGTTTCTGGAAAGGATGTAATTATATTGTGCTGGATTATGTTGTTGCTGTGCCCTGGATTGTTGCCTCTGATCGAATATCATACAACTGGATCTGTAGAATATTTGTGCTTGTCTTCATGGTTTTTCTTTATCTTAATGTATTTATTATTGTCCCTCACCTAATAAGAATCTTTGTATCCTATGGGACAGCGAAAATTCATACCAATTTGTAA

Protein sequence

>Msa0291880

MSHNINQQQSPVTAYPAVSQNNPQAVPPPPMGYPSKDDPQQSVPIKTTSRGDGFWKGCNYIVLDYVVAVPWIVASDRISYNWICRIFVLVFMVFLYLNVFIIVPHLIRIFVSYGTAKIHTNL*