AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0117200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117200 MtrunA17_Chr3g0084311 100.000 87 0 0 1 87 1 87 3.05e-63 185
Msa0117200 MtrunA17_Chr3g0084301 100.000 87 0 0 1 87 1 87 3.05e-63 185
Msa0117200 MtrunA17_Chr8g0364261 97.701 87 2 0 1 87 1 87 1.08e-61 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117200 AT4G14342.1 89.655 87 9 0 1 87 1 87 6.87e-58 172
Msa0117200 AT3G23325.1 87.356 87 11 0 1 87 1 87 9.07e-57 169
Msa0117200 AT4G14342.2 86.250 80 11 0 1 80 1 80 3.84e-49 150

Find 38 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAATCTTCTTCTCTTTC+AGG 0.133068 1_3:-37554498 None:intergenic
GATAGCTATGCTTCGTATAT+TGG 0.282004 1_3:+37553167 Msa0117200:CDS
TGTTGGAGATTAGTCTAAAC+AGG 0.316640 1_3:+37554730 Msa0117200:three_prime_UTR
TACTTTAGTAGCATAGAAAT+AGG 0.321750 1_3:+37554771 Msa0117200:three_prime_UTR
GGATGAACAGTGCTGCGATT+TGG 0.341196 1_3:-37551369 None:intergenic
GATGAACAGTGCTGCGATTT+GGG 0.346664 1_3:-37551368 None:intergenic
ATTGCTCAAAACGAATCTAT+TGG 0.369668 1_3:+37553218 Msa0117200:CDS
TAGAAAAGTAAGAAAGTAAA+GGG 0.419141 1_3:-37553196 None:intergenic
GCAATATGCAGGCGAGTGAT+AGG 0.420071 1_3:+37553045 Msa0117200:exon
CGGTGAAGAACAGAGGGATT+TGG 0.423414 1_3:-37551343 None:intergenic
CAAGCTAAATATGTTGGAAC+TGG 0.450379 1_3:+37553098 Msa0117200:CDS
CTCTCCAACAAGTCACGAAC+TGG 0.452985 1_3:-37553022 None:intergenic
ATAGAAAAGTAAGAAAGTAA+AGG 0.454525 1_3:-37553197 None:intergenic
ATTAGTCTAAACAGGGAACT+TGG 0.470276 1_3:+37554738 Msa0117200:three_prime_UTR
AAGAAGATTAGAATTATCGT+TGG 0.471758 1_3:+37554508 Msa0117200:exon
CTGATATCAATAGATTTGAA+TGG 0.473717 1_3:+37553126 Msa0117200:CDS
AAATTATACAGTACAACATA+TGG 0.481227 1_3:-37554561 None:intergenic
AGCTTGCAGATGCTCGAGTT+GGG 0.481702 1_3:-37553081 None:intergenic
AGAAGATTAGAATTATCGTT+GGG 0.491177 1_3:+37554509 Msa0117200:exon
TGGAATTTGACTTACAGTTA+CGG 0.491517 1_3:-37551390 None:intergenic
AGTTCCAGTTCGTGACTTGT+TGG 0.491647 1_3:+37553018 Msa0117200:intron
GGATTTGGTTCAGCTTCAAT+CGG 0.501068 1_3:-37551328 None:intergenic
AGTAAGAAAGTAAAGGGTAA+TGG 0.502423 1_3:-37553190 None:intergenic
TAGCTTGCAGATGCTCGAGT+TGG 0.512932 1_3:-37553082 None:intergenic
CATCTGCAAGCTAAATATGT+TGG 0.518362 1_3:+37553092 Msa0117200:CDS
ATTATCGTTGGGATCATCAC+AGG 0.545841 1_3:+37554520 Msa0117200:three_prime_UTR
CAGGAGCAAGACCACATGGC+AGG 0.553543 1_3:-37554479 None:intergenic
CAGTTCGTGACTTGTTGGAG+AGG 0.557579 1_3:+37553023 Msa0117200:five_prime_UTR
TGATATCAATAGATTTGAAT+GGG 0.559675 1_3:+37553127 Msa0117200:CDS
TTGTTGGAGAGGCAATATGC+AGG 0.582739 1_3:+37553034 Msa0117200:exon
CTTTCAGGAGCAAGACCACA+TGG 0.584496 1_3:-37554483 None:intergenic
TGGGAACGGTGAAGAACAGA+GGG 0.610035 1_3:-37551349 None:intergenic
ATCATCACAGGAGAGTTGAA+AGG 0.629634 1_3:+37554532 Msa0117200:three_prime_UTR
TTGGGAACGGTGAAGAACAG+AGG 0.658307 1_3:-37551350 None:intergenic
TGTACTGTATAATTTGATCG+AGG 0.659272 1_3:+37554569 Msa0117200:three_prime_UTR
GTTGGAGATTAGTCTAAACA+GGG 0.672427 1_3:+37554731 Msa0117200:three_prime_UTR
CAGAAAATGCTCCTGCCATG+TGG 0.674077 1_3:+37554468 Msa0117200:intron
ACAGTGCTGCGATTTGGGAA+CGG 0.686831 1_3:-37551363 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAGATATATAACATTTA+AGG - chr1_3:37552964-37552983 None:intergenic 10.0%
!! ATACTTAAGTAAATTAAAAT+TGG - chr1_3:37553911-37553930 None:intergenic 10.0%
!!! AAAAAAAAAAAAAAACCTTT+TGG - chr1_3:37554189-37554208 None:intergenic 10.0%
!! GATTTATATAAAAGAAAGAA+AGG + chr1_3:37552325-37552344 Msa0117200:intron 15.0%
!!! TTTTGCAAAATAATTCTATA+TGG + chr1_3:37552607-37552626 Msa0117200:intron 15.0%
!! GATTCATATAACATATAATT+TGG + chr1_3:37552685-37552704 Msa0117200:intron 15.0%
!! TGTAAATTATATGAAAATTG+TGG - chr1_3:37552817-37552836 None:intergenic 15.0%
!! TATGAATTTATATGCATATA+TGG + chr1_3:37553978-37553997 Msa0117200:intron 15.0%
!! ATGAATTTATATGCATATAT+GGG + chr1_3:37553979-37553998 Msa0117200:intron 15.0%
!! AATAAAAATTGAATAAATCC+AGG - chr1_3:37554355-37554374 None:intergenic 15.0%
!!! TGATTTATATGTTATCTTTT+TGG - chr1_3:37554811-37554830 None:intergenic 15.0%
!!! TGTTTTTATTAGCAAAATCA+GGG - chr1_3:37551294-37551313 None:intergenic 20.0%
!!! ATGTTTTTATTAGCAAAATC+AGG - chr1_3:37551295-37551314 None:intergenic 20.0%
!!! TGTTTTTACTATAACATGTT+AGG + chr1_3:37551504-37551523 Msa0117200:intron 20.0%
!!! CGAAAAGTGATTTTATTTTA+TGG + chr1_3:37551558-37551577 Msa0117200:intron 20.0%
!!! TTATTTTATGGAGATTTTGT+AGG + chr1_3:37551570-37551589 Msa0117200:intron 20.0%
!!! AAAGCAATATTACTTGATTT+TGG + chr1_3:37551753-37551772 Msa0117200:intron 20.0%
!! ATGTAGAATATTGATATGTT+TGG - chr1_3:37552542-37552561 None:intergenic 20.0%
!!! TTATATTTTTCACTAGCTTT+TGG + chr1_3:37552636-37552655 Msa0117200:intron 20.0%
!! AATAACCAAATGAATTAACA+AGG - chr1_3:37552764-37552783 None:intergenic 20.0%
!! TCATATAATTTACAGTTACT+TGG + chr1_3:37552823-37552842 Msa0117200:intron 20.0%
!!! TTAGTACTAGTTTTAATCAA+TGG + chr1_3:37552884-37552903 Msa0117200:intron 20.0%
!! TGATATCAATAGATTTGAAT+GGG + chr1_3:37553127-37553146 Msa0117200:CDS 20.0%
!! TAGAAAAGTAAGAAAGTAAA+GGG - chr1_3:37553199-37553218 None:intergenic 20.0%
!! ATAGAAAAGTAAGAAAGTAA+AGG - chr1_3:37553200-37553219 None:intergenic 20.0%
!! AAAGAAAATAGTAACGATAT+AGG - chr1_3:37553312-37553331 None:intergenic 20.0%
!!! ATCCTATTATTTCTAAATCA+CGG - chr1_3:37553627-37553646 None:intergenic 20.0%
!!! GTATTTTATTAGAAAGAATG+GGG - chr1_3:37553715-37553734 None:intergenic 20.0%
!!! GGTATTTTATTAGAAAGAAT+GGG - chr1_3:37553716-37553735 None:intergenic 20.0%
!!! TGGTATTTTATTAGAAAGAA+TGG - chr1_3:37553717-37553736 None:intergenic 20.0%
!! AATTAAAATTGGTAAGCATA+AGG - chr1_3:37553900-37553919 None:intergenic 20.0%
!!! ATTGAGATTTTACACAAAAA+CGG - chr1_3:37554041-37554060 None:intergenic 20.0%
!! TTGATGAAAAATTCTTAAGA+AGG - chr1_3:37554072-37554091 None:intergenic 20.0%
!! AATAAAAATTGAATAAACCC+AGG - chr1_3:37554276-37554295 None:intergenic 20.0%
!! TTACTTGATTGATTGAAATA+TGG + chr1_3:37554301-37554320 Msa0117200:intron 20.0%
!!! AAATTAGAATATAAAGTGCT+AGG - chr1_3:37554428-37554447 None:intergenic 20.0%
!! AAATTATACAGTACAACATA+TGG - chr1_3:37554564-37554583 None:intergenic 20.0%
!!! TTATATGTTATCTTTTTGGA+GGG - chr1_3:37554807-37554826 None:intergenic 20.0%
!!! TTTATATGTTATCTTTTTGG+AGG - chr1_3:37554808-37554827 None:intergenic 20.0%
! GATGACTTATGAAAATAAGT+TGG - chr1_3:37551697-37551716 None:intergenic 25.0%
! TTTGTCACCATAATTATTCA+GGG - chr1_3:37551956-37551975 None:intergenic 25.0%
!! TTTTGTCACCATAATTATTC+AGG - chr1_3:37551957-37551976 None:intergenic 25.0%
! AAGAGATTTAAGAGAGAATA+CGG + chr1_3:37552073-37552092 Msa0117200:intron 25.0%
! TTTAGTGTAATTACATGTGT+CGG + chr1_3:37552214-37552233 Msa0117200:intron 25.0%
! TATAAAAGAAAGAAAGGTTG+TGG + chr1_3:37552331-37552350 Msa0117200:intron 25.0%
! TAGATCCTTGTTAATTCATT+TGG + chr1_3:37552756-37552775 Msa0117200:intron 25.0%
! CTGATATCAATAGATTTGAA+TGG + chr1_3:37553126-37553145 Msa0117200:CDS 25.0%
! TCTATAACATAGTAGAAATC+TGG - chr1_3:37553737-37553756 None:intergenic 25.0%
!! TACGGATTTTTATAATGTTG+AGG - chr1_3:37554102-37554121 None:intergenic 25.0%
! CAACATTATAAAAATCCGTA+TGG + chr1_3:37554102-37554121 Msa0117200:intron 25.0%
! AAAGTGGTGACAAAAATATT+TGG + chr1_3:37554128-37554147 Msa0117200:intron 25.0%
! AAGAAGATTAGAATTATCGT+TGG + chr1_3:37554508-37554527 Msa0117200:exon 25.0%
! AGAAGATTAGAATTATCGTT+GGG + chr1_3:37554509-37554528 Msa0117200:exon 25.0%
! TACTTTAGTAGCATAGAAAT+AGG + chr1_3:37554771-37554790 Msa0117200:three_prime_UTR 25.0%
! TGGAATTTGACTTACAGTTA+CGG - chr1_3:37551393-37551412 None:intergenic 30.0%
AACGGAAGAAAAGTGAAATT+TGG - chr1_3:37551413-37551432 None:intergenic 30.0%
CATGGCATAGAAAGTAAAAA+CGG - chr1_3:37551431-37551450 None:intergenic 30.0%
ATGAAATTGAACTACCCTAT+TGG - chr1_3:37551461-37551480 None:intergenic 30.0%
! TATGGAGATTTTGTAGGTTT+TGG + chr1_3:37551576-37551595 Msa0117200:intron 30.0%
!! AAGCTGTTTTCATAAGTTCT+TGG - chr1_3:37551652-37551671 None:intergenic 30.0%
TTTCATAAGTCATCGAAGAT+AGG + chr1_3:37551703-37551722 Msa0117200:intron 30.0%
AGTTAAATCAGTATCTTCGA+TGG - chr1_3:37551875-37551894 None:intergenic 30.0%
ACAATGTGATGTGAACAATA+AGG + chr1_3:37551999-37552018 Msa0117200:intron 30.0%
TCTCAAATTATTAGCGATGT+CGG - chr1_3:37552183-37552202 None:intergenic 30.0%
TATCTCACAACTAACTACAT+TGG - chr1_3:37552494-37552513 None:intergenic 30.0%
GTGAGATACTAATATTGTGA+AGG + chr1_3:37552506-37552525 Msa0117200:intron 30.0%
AGTAAGAAAGTAAAGGGTAA+TGG - chr1_3:37553193-37553212 None:intergenic 30.0%
ATTGCTCAAAACGAATCTAT+TGG + chr1_3:37553218-37553237 Msa0117200:CDS 30.0%
TGATGTCAAAACATTGAACT+TGG + chr1_3:37553364-37553383 Msa0117200:intron 30.0%
TCAAAACATTGAACTTGGTT+AGG + chr1_3:37553369-37553388 Msa0117200:intron 30.0%
GACCGTGATTTAGAAATAAT+AGG + chr1_3:37553622-37553641 Msa0117200:intron 30.0%
AATAATAGGATACGATGAGA+AGG + chr1_3:37553636-37553655 Msa0117200:intron 30.0%
TACTATGTTATAGAGCATGA+GGG + chr1_3:37553743-37553762 Msa0117200:intron 30.0%
! GATTTTACACAAAAACGGAA+AGG - chr1_3:37554036-37554055 None:intergenic 30.0%
! AAAATCCGTATGGATTAAAG+TGG + chr1_3:37554112-37554131 Msa0117200:intron 30.0%
!!! TTTTGGTTCTCCAAAGATTA+GGG - chr1_3:37554172-37554191 None:intergenic 30.0%
! AGGTGAGATTATAAAGTATC+AGG - chr1_3:37554335-37554354 None:intergenic 30.0%
TGATACTTTATAATCTCACC+TGG + chr1_3:37554334-37554353 Msa0117200:intron 30.0%
TTCTAATCTTCTTCTCTTTC+AGG - chr1_3:37554501-37554520 None:intergenic 30.0%
TGTACTGTATAATTTGATCG+AGG + chr1_3:37554569-37554588 Msa0117200:three_prime_UTR 30.0%
!!! CTTAACTTTGCCTGTTTTTA+TGG + chr1_3:37554639-37554658 Msa0117200:three_prime_UTR 30.0%
! GTACTTTTCATGAATTGTGT+TGG + chr1_3:37554713-37554732 Msa0117200:three_prime_UTR 30.0%
ATGCCATGATTCAATCCAAT+AGG + chr1_3:37551443-37551462 Msa0117200:intron 35.0%
TGCCATGATTCAATCCAATA+GGG + chr1_3:37551444-37551463 Msa0117200:intron 35.0%
TACCCTATTGGATTGAATCA+TGG - chr1_3:37551449-37551468 None:intergenic 35.0%
AGTTCTTGGCAAACAACTTA+TGG - chr1_3:37551638-37551657 None:intergenic 35.0%
ATCCTTCTCAAAGGGTAAAT+GGG - chr1_3:37551905-37551924 None:intergenic 35.0%
TATCCTTCTCAAAGGGTAAA+TGG - chr1_3:37551906-37551925 None:intergenic 35.0%
TAATTATTCAGGGTTTGGCA+GGG - chr1_3:37551946-37551965 None:intergenic 35.0%
ATAATTATTCAGGGTTTGGC+AGG - chr1_3:37551947-37551966 None:intergenic 35.0%
TGCCAAACCCTGAATAATTA+TGG + chr1_3:37551946-37551965 Msa0117200:intron 35.0%
CACCATAATTATTCAGGGTT+TGG - chr1_3:37551951-37551970 None:intergenic 35.0%
AGGAAAAGTTAGAGAAGTGA+AGG + chr1_3:37552019-37552038 Msa0117200:intron 35.0%
TTGATGAGTGATGACTAACA+AGG - chr1_3:37552465-37552484 None:intergenic 35.0%
CATCTGCAAGCTAAATATGT+TGG + chr1_3:37553092-37553111 Msa0117200:CDS 35.0%
CAAGCTAAATATGTTGGAAC+TGG + chr1_3:37553098-37553117 Msa0117200:CDS 35.0%
GATAGCTATGCTTCGTATAT+TGG + chr1_3:37553167-37553186 Msa0117200:CDS 35.0%
TTGACACATCAACTTGTAGT+AGG + chr1_3:37553407-37553426 Msa0117200:intron 35.0%
AAGCGATTTGACAGAGAATT+GGG + chr1_3:37553667-37553686 Msa0117200:intron 35.0%
CTACTATGTTATAGAGCATG+AGG + chr1_3:37553742-37553761 Msa0117200:intron 35.0%
TCTAGAAGAGATTGACGATT+AGG + chr1_3:37553784-37553803 Msa0117200:intron 35.0%
TGTCACCACTTTAATCCATA+CGG - chr1_3:37554120-37554139 None:intergenic 35.0%
GTGGTGACAAAAATATTTGG+AGG + chr1_3:37554131-37554150 Msa0117200:intron 35.0%
!!! CTTTTGGTTCTCCAAAGATT+AGG - chr1_3:37554173-37554192 None:intergenic 35.0%
! CTAATCTTTGGAGAACCAAA+AGG + chr1_3:37554171-37554190 Msa0117200:intron 35.0%
! ATTAGCTGTTGTTGTTAGTG+TGG + chr1_3:37554221-37554240 Msa0117200:intron 35.0%
! TTAGCTGTTGTTGTTAGTGT+GGG + chr1_3:37554222-37554241 Msa0117200:intron 35.0%
!!! CTTTGCCTGTTTTTATGGTA+TGG + chr1_3:37554644-37554663 Msa0117200:three_prime_UTR 35.0%
AAGCTCCATACCATAAAAAC+AGG - chr1_3:37554652-37554671 None:intergenic 35.0%
! GTTTTTATGGTATGGAGCTT+CGG + chr1_3:37554652-37554671 Msa0117200:three_prime_UTR 35.0%
!! TGTTGGAGATTAGTCTAAAC+AGG + chr1_3:37554730-37554749 Msa0117200:three_prime_UTR 35.0%
!! GTTGGAGATTAGTCTAAACA+GGG + chr1_3:37554731-37554750 Msa0117200:three_prime_UTR 35.0%
! ATTAGTCTAAACAGGGAACT+TGG + chr1_3:37554738-37554757 Msa0117200:three_prime_UTR 35.0%
! GGATTTGGTTCAGCTTCAAT+CGG - chr1_3:37551331-37551350 None:intergenic 40.0%
! TTCCCATTTACCCTTTGAGA+AGG + chr1_3:37551900-37551919 Msa0117200:intron 40.0%
GCTTTGGTATCCTTCTCAAA+GGG - chr1_3:37551913-37551932 None:intergenic 40.0%
! AGCTTTGGTATCCTTCTCAA+AGG - chr1_3:37551914-37551933 None:intergenic 40.0%
AATTATTCAGGGTTTGGCAG+GGG - chr1_3:37551945-37551964 None:intergenic 40.0%
GTTAGAGAAGTGAAGGCTTA+AGG + chr1_3:37552026-37552045 Msa0117200:intron 40.0%
! GAGAGATTGGTTGTGATAGA+GGG + chr1_3:37552048-37552067 Msa0117200:intron 40.0%
! GTAATTACATGTGTCGGTGT+CGG + chr1_3:37552220-37552239 Msa0117200:intron 40.0%
ACCTTCTTGCCTATAAAGCA+CGG - chr1_3:37552292-37552311 None:intergenic 40.0%
! TGCATTTTTCCTTCCCTGTT+TGG + chr1_3:37552421-37552440 Msa0117200:intron 40.0%
! TTTTCCTTCCCTGTTTGGTT+TGG + chr1_3:37552426-37552445 Msa0117200:intron 40.0%
!! TTCCCTGTTTGGTTTGGTTT+CGG + chr1_3:37552432-37552451 Msa0117200:intron 40.0%
CTCACAACTAACTACATTGG+TGG - chr1_3:37552491-37552510 None:intergenic 40.0%
TACTTGGTTCACACTTTGAG+AGG + chr1_3:37552839-37552858 Msa0117200:intron 40.0%
! ACTTGGTTCACACTTTGAGA+GGG + chr1_3:37552840-37552859 Msa0117200:intron 40.0%
! ACAGCGTTACACTTTTATGC+AGG + chr1_3:37553244-37553263 Msa0117200:CDS 40.0%
TATTTCGCTATTGCCTGCTA+TGG - chr1_3:37553480-37553499 None:intergenic 40.0%
! CTAAAGTGTTTGCCGCAAAT+AGG + chr1_3:37553529-37553548 Msa0117200:intron 40.0%
CAAATAACCGACCAGAGTTT+GGG - chr1_3:37553575-37553594 None:intergenic 40.0%
TCTGGTCGGTTATTTGGAAA+TGG + chr1_3:37553579-37553598 Msa0117200:intron 40.0%
CAAGCGATTTGACAGAGAAT+TGG + chr1_3:37553666-37553685 Msa0117200:intron 40.0%
AGCGATTTGACAGAGAATTG+GGG + chr1_3:37553668-37553687 Msa0117200:intron 40.0%
TATGTTATAGAGCATGAGGG+TGG + chr1_3:37553746-37553765 Msa0117200:intron 40.0%
! TCGTCAATCTCTTCTAGAGA+AGG - chr1_3:37553782-37553801 None:intergenic 40.0%
GATTGACGATTAGGTGTTCT+TGG + chr1_3:37553793-37553812 Msa0117200:intron 40.0%
TGTAGTGACACCCTAATCTT+TGG + chr1_3:37554159-37554178 Msa0117200:intron 40.0%
! TAGCTGTTGTTGTTAGTGTG+GGG + chr1_3:37554223-37554242 Msa0117200:intron 40.0%
AGGAGTGATAAGGAGGTAAT+GGG - chr1_3:37554256-37554275 None:intergenic 40.0%
ATTACCTCCTTATCACTCCT+GGG + chr1_3:37554256-37554275 Msa0117200:intron 40.0%
GAATAAACCCAGGAGTGATA+AGG - chr1_3:37554266-37554285 None:intergenic 40.0%
CTAGGTGTGATTATAGTGTC+AGG - chr1_3:37554410-37554429 None:intergenic 40.0%
ATTATCGTTGGGATCATCAC+AGG + chr1_3:37554520-37554539 Msa0117200:three_prime_UTR 40.0%
ATCATCACAGGAGAGTTGAA+AGG + chr1_3:37554532-37554551 Msa0117200:three_prime_UTR 40.0%
GATGAACAGTGCTGCGATTT+GGG - chr1_3:37551371-37551390 None:intergenic 45.0%
GTGAAGGCTTAAGGAGAGAT+TGG + chr1_3:37552035-37552054 Msa0117200:intron 45.0%
! GGAGAGATTGGTTGTGATAG+AGG + chr1_3:37552047-37552066 Msa0117200:intron 45.0%
TATAAAGCACGGACACTCCT+CGG - chr1_3:37552281-37552300 None:intergenic 45.0%
TCCGTGCTTTATAGGCAAGA+AGG + chr1_3:37552288-37552307 Msa0117200:intron 45.0%
GGCAAGAAGGTAGTGAAGAA+AGG + chr1_3:37552301-37552320 Msa0117200:intron 45.0%
ACGAGAGAAGTAGAGATGCT+AGG + chr1_3:37552378-37552397 Msa0117200:intron 45.0%
GAAACCAAACCAAACAGGGA+AGG - chr1_3:37552433-37552452 None:intergenic 45.0%
CACCGAAACCAAACCAAACA+GGG - chr1_3:37552437-37552456 None:intergenic 45.0%
ACACCGAAACCAAACCAAAC+AGG - chr1_3:37552438-37552457 None:intergenic 45.0%
! AGTTCCAGTTCGTGACTTGT+TGG + chr1_3:37553018-37553037 Msa0117200:intron 45.0%
TTGTTGGAGAGGCAATATGC+AGG + chr1_3:37553034-37553053 Msa0117200:exon 45.0%
AGCAGGCAATAGCGAAATAG+CGG + chr1_3:37553481-37553500 Msa0117200:intron 45.0%
CCAAATAACCGACCAGAGTT+TGG - chr1_3:37553576-37553595 None:intergenic 45.0%
CCAAACTCTGGTCGGTTATT+TGG + chr1_3:37553573-37553592 Msa0117200:intron 45.0%
GTCGGTTATTTGGAAATGGC+AGG + chr1_3:37553583-37553602 Msa0117200:intron 45.0%
GGATATGAATCCCAACCCAA+AGG - chr1_3:37553694-37553713 None:intergenic 45.0%
CAGGAGTGATAAGGAGGTAA+TGG - chr1_3:37554257-37554276 None:intergenic 45.0%
CATTACCTCCTTATCACTCC+TGG + chr1_3:37554255-37554274 Msa0117200:intron 45.0%
TAAACCCAGGAGTGATAAGG+AGG - chr1_3:37554263-37554282 None:intergenic 45.0%
CGGTGAAGAACAGAGGGATT+TGG - chr1_3:37551346-37551365 None:intergenic 50.0%
TGGGAACGGTGAAGAACAGA+GGG - chr1_3:37551352-37551371 None:intergenic 50.0%
TTGGGAACGGTGAAGAACAG+AGG - chr1_3:37551353-37551372 None:intergenic 50.0%
! ACAGTGCTGCGATTTGGGAA+CGG - chr1_3:37551366-37551385 None:intergenic 50.0%
GGATGAACAGTGCTGCGATT+TGG - chr1_3:37551372-37551391 None:intergenic 50.0%
ACGAACACGAACACGAACAC+CGG + chr1_3:37552135-37552154 Msa0117200:intron 50.0%
ACACAACACGTGTTCGACAC+CGG + chr1_3:37552243-37552262 Msa0117200:intron 50.0%
!! GAGGAGTGTCCGTGCTTTAT+AGG + chr1_3:37552280-37552299 Msa0117200:intron 50.0%
CTCTCCAACAAGTCACGAAC+TGG - chr1_3:37553025-37553044 None:intergenic 50.0%
!! CAGTTCGTGACTTGTTGGAG+AGG + chr1_3:37553023-37553042 Msa0117200:five_prime_UTR 50.0%
GCAATATGCAGGCGAGTGAT+AGG + chr1_3:37553045-37553064 Msa0117200:exon 50.0%
AGCTTGCAGATGCTCGAGTT+GGG - chr1_3:37553084-37553103 None:intergenic 50.0%
! TAGCTTGCAGATGCTCGAGT+TGG - chr1_3:37553085-37553104 None:intergenic 50.0%
! GCAATAGCGAAATAGCGGAG+AGG + chr1_3:37553486-37553505 Msa0117200:intron 50.0%
! GCGAAATAGCGGAGAGGATT+AGG + chr1_3:37553492-37553511 Msa0117200:intron 50.0%
TGACAGAGAATTGGGGCCTT+TGG + chr1_3:37553675-37553694 Msa0117200:intron 50.0%
GACAGAGAATTGGGGCCTTT+GGG + chr1_3:37553676-37553695 Msa0117200:intron 50.0%
! AGAATTGGGGCCTTTGGGTT+GGG + chr1_3:37553681-37553700 Msa0117200:intron 50.0%
CAGAAAATGCTCCTGCCATG+TGG + chr1_3:37554468-37554487 Msa0117200:intron 50.0%
CTTTCAGGAGCAAGACCACA+TGG - chr1_3:37554486-37554505 None:intergenic 50.0%
! GCAGGGGACTTTGGTAGCTT+TGG - chr1_3:37551929-37551948 None:intergenic 55.0%
GGTGTTCGTGTTCGTGTTCG+TGG - chr1_3:37552136-37552155 None:intergenic 55.0%
CACGAACACCGGACACATCA+CGG + chr1_3:37552146-37552165 Msa0117200:intron 55.0%
GTCAGTGTCCGTGATGTGTC+CGG - chr1_3:37552157-37552176 None:intergenic 55.0%
CACAACACGTGTTCGACACC+GGG + chr1_3:37552244-37552263 Msa0117200:intron 55.0%
! TTTGCCGCAAATAGGCGTCG+AGG + chr1_3:37553537-37553556 Msa0117200:intron 55.0%
CTCTCCTCGACGCCTATTTG+CGG - chr1_3:37553544-37553563 None:intergenic 55.0%
!! ATAGGCGTCGAGGAGAGTGT+CGG + chr1_3:37553547-37553566 Msa0117200:intron 55.0%
!! GAGTGTCGGTACCCAAACTC+TGG + chr1_3:37553561-37553580 Msa0117200:intron 55.0%
GTCGGTACCCAAACTCTGGT+CGG + chr1_3:37553565-37553584 Msa0117200:intron 55.0%
GAGAATTGGGGCCTTTGGGT+TGG + chr1_3:37553680-37553699 Msa0117200:intron 55.0%
CAGGGTTTGGCAGGGGACTT+TGG - chr1_3:37551938-37551957 None:intergenic 60.0%
! TCCTCGGATTAGGCGTGTCC+CGG - chr1_3:37552265-37552284 None:intergenic 60.0%
GCACGGACACTCCTCGGATT+AGG - chr1_3:37552275-37552294 None:intergenic 60.0%
CTGTGCTGTGCTGCCATAGC+AGG + chr1_3:37553464-37553483 Msa0117200:intron 60.0%
CAGGAGCAAGACCACATGGC+AGG - chr1_3:37554482-37554501 None:intergenic 60.0%
ACCGGGACACGCCTAATCCG+AGG + chr1_3:37552261-37552280 Msa0117200:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 37551273 37554835 37551273 ID=Msa0117200;Name=Msa0117200
chr1_3 mRNA 37551273 37554835 37551273 ID=Msa0117200-mRNA-1;Parent=Msa0117200;Name=Msa0117200-mRNA-1;_AED=0.01;_eAED=0.01;_QI=155|1|1|1|0.5|0.66|3|317|87
chr1_3 exon 37551273 37551397 37551273 ID=Msa0117200-mRNA-1:exon:11881;Parent=Msa0117200-mRNA-1
chr1_3 exon 37553020 37553265 37553020 ID=Msa0117200-mRNA-1:exon:11882;Parent=Msa0117200-mRNA-1
chr1_3 exon 37554471 37554835 37554471 ID=Msa0117200-mRNA-1:exon:11883;Parent=Msa0117200-mRNA-1
chr1_3 five_prime_UTR 37551273 37551397 37551273 ID=Msa0117200-mRNA-1:five_prime_utr;Parent=Msa0117200-mRNA-1
chr1_3 five_prime_UTR 37553020 37553049 37553020 ID=Msa0117200-mRNA-1:five_prime_utr;Parent=Msa0117200-mRNA-1
chr1_3 CDS 37553050 37553265 37553050 ID=Msa0117200-mRNA-1:cds;Parent=Msa0117200-mRNA-1
chr1_3 CDS 37554471 37554518 37554471 ID=Msa0117200-mRNA-1:cds;Parent=Msa0117200-mRNA-1
chr1_3 three_prime_UTR 37554519 37554835 37554519 ID=Msa0117200-mRNA-1:three_prime_utr;Parent=Msa0117200-mRNA-1
Gene Sequence

>Msa0117200

ATGCAGGCGAGTGATAGGTTTAACATAAATTCCCAACTCGAGCATCTGCAAGCTAAATATGTTGGAACTGGTCATGCTGATATCAATAGATTTGAATGGGCTGTGAATATTCAACGTGATAGCTATGCTTCGTATATTGGCCATTACCCTTTACTTTCTTACTTTTCTATTGCTCAAAACGAATCTATTGGTAGACAGCGTTACACTTTTATGCAGAAAATGCTCCTGCCATGTGGTCTTGCTCCTGAAAGAGAAGAAGATTAG
Protein sequence

>Msa0117200

MQASDRFNINSQLEHLQAKYVGTGHADINRFEWAVNIQRDSYASYIGHYPLLSYFSIAQNESIGRQRYTFMQKMLLPCGLAPEREED*