Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0003220 | Msa0117920 | 0.805441 | 1.332868e-49 | -8.615850e-47 |
| Msa0064920 | Msa0117920 | 0.812214 | 4.743718e-51 | -8.615850e-47 |
| Msa0117910 | Msa0117920 | 0.838140 | 3.431100e-57 | -8.615850e-47 |
| Msa0117920 | Msa0126230 | 0.819601 | 1.066015e-52 | -8.615850e-47 |
| Msa0117920 | Msa0177170 | 0.805803 | 1.118854e-49 | -8.615850e-47 |
| Msa0117920 | Msa0279390 | 0.813830 | 2.098759e-51 | -8.615850e-47 |
| Msa0117920 | Msa0318880 | 0.810981 | 8.794312e-51 | -8.615850e-47 |
| Msa0117920 | Msa0423840 | 0.820530 | 6.533152e-53 | -8.615850e-47 |
| Msa0117920 | Msa0446900 | 0.814452 | 1.529352e-51 | -8.615850e-47 |
| Msa0117920 | Msa0689420 | 0.802740 | 4.860180e-49 | -8.615850e-47 |
| Msa0117920 | Msa0749400 | 0.809818 | 1.567560e-50 | -8.615850e-47 |
| Msa0117920 | Msa1329790 | 0.800696 | 1.276668e-48 | -8.615850e-47 |
| Msa0117920 | Msa1338250 | 0.807554 | 4.775001e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTATAGATGCTTGAGTTT+TGG | 0.320360 | 1_3:-40008392 | Msa0117920:CDS |
| TTGAAGTTAGCGTCGGAAAA+CGG | 0.466817 | 1_3:+40008562 | None:intergenic |
| TCAGGATCAATCTTCGACCT+TGG | 0.499943 | 1_3:+40008346 | None:intergenic |
| GAAAGTGTGAGTGAGGAAGA+TGG | 0.568365 | 1_3:+40008677 | None:intergenic |
| AAGAGAAGAGAGAAACGAAG+TGG | 0.586874 | 1_3:+40008631 | None:intergenic |
| GGAAAACGGTGAGAAGAAGA+AGG | 0.603048 | 1_3:+40008576 | None:intergenic |
| CAATGGCGTCGAAAGGTCCA+AGG | 0.603635 | 1_3:-40008363 | Msa0117920:CDS |
| TCGTTCGTTGAAGTTAGCGT+CGG | 0.605241 | 1_3:+40008555 | None:intergenic |
| GTGAATGCAATGGCGTCGAA+AGG | 0.607430 | 1_3:-40008370 | Msa0117920:CDS |
| GTTGTGAAGAGCGTTGAGAA+TGG | 0.609672 | 1_3:+40008653 | None:intergenic |
| GAATGGTGAAAGTGTGAGTG+AGG | 0.626805 | 1_3:+40008670 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATAGATGCTTGAGTTT+TGG | - | chr1_3:40008645-40008664 | Msa0117920:CDS | 25.0% |
| !!! | TTCTACTTTTTCACTCTTCA+CGG | - | chr1_3:40008506-40008525 | Msa0117920:intron | 30.0% |
| ! | TTTTATTCACGCATCGAATT+AGG | - | chr1_3:40008560-40008579 | Msa0117920:CDS | 30.0% |
| !! | TTGAGTTTTGGTGAATGCAA+TGG | - | chr1_3:40008657-40008676 | Msa0117920:CDS | 35.0% |
| AAGAGAAGAGAGAAACGAAG+TGG | + | chr1_3:40008409-40008428 | None:intergenic | 40.0% | |
| ! | TTGAAGTTAGCGTCGGAAAA+CGG | + | chr1_3:40008478-40008497 | None:intergenic | 40.0% |
| ! | GAAAGTGTGAGTGAGGAAGA+TGG | + | chr1_3:40008363-40008382 | None:intergenic | 45.0% |
| ! | GAATGGTGAAAGTGTGAGTG+AGG | + | chr1_3:40008370-40008389 | None:intergenic | 45.0% |
| !! | GTTGTGAAGAGCGTTGAGAA+TGG | + | chr1_3:40008387-40008406 | None:intergenic | 45.0% |
| GGAAAACGGTGAGAAGAAGA+AGG | + | chr1_3:40008464-40008483 | None:intergenic | 45.0% | |
| TCGTTCGTTGAAGTTAGCGT+CGG | + | chr1_3:40008485-40008504 | None:intergenic | 45.0% | |
| ! | GTGAATGCAATGGCGTCGAA+AGG | - | chr1_3:40008667-40008686 | Msa0117920:CDS | 50.0% |
| ! | CAATGGCGTCGAAAGGTCCA+AGG | - | chr1_3:40008674-40008693 | Msa0117920:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 40008355 | 40008704 | 40008355 | ID=Msa0117920;Name=Msa0117920 |
| chr1_3 | mRNA | 40008355 | 40008704 | 40008355 | ID=Msa0117920-mRNA-1;Parent=Msa0117920;Name=Msa0117920-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|1|0|1|0|0|2|0|78 |
| chr1_3 | exon | 40008355 | 40008415 | 40008355 | ID=Msa0117920-mRNA-1:exon:12251;Parent=Msa0117920-mRNA-1 |
| chr1_3 | exon | 40008532 | 40008704 | 40008532 | ID=Msa0117920-mRNA-1:exon:12250;Parent=Msa0117920-mRNA-1 |
| chr1_3 | CDS | 40008532 | 40008704 | 40008532 | ID=Msa0117920-mRNA-1:cds;Parent=Msa0117920-mRNA-1 |
| chr1_3 | CDS | 40008355 | 40008415 | 40008355 | ID=Msa0117920-mRNA-1:cds;Parent=Msa0117920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
