AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0129990

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129990 MtrunA17_Chr1g0197171 96.333 300 6 2 1 300 1 295 0.0 599
Msa0129990 MtrunA17_Chr2g0290991 69.040 323 67 6 1 300 1 313 2.69e-150 423
Msa0129990 MtrunA17_Chr8g0356501 52.096 167 67 4 29 191 17 174 5.84e-46 158
Msa0129990 MtrunA17_Chr2g0310011 57.534 146 50 3 38 180 24 160 2.52e-44 154
Msa0129990 MtrunA17_Chr5g0409731 52.980 151 63 4 42 186 18 166 4.01e-43 149
Msa0129990 MtrunA17_Chr5g0417521 50.704 142 67 1 42 180 8 149 2.98e-39 140
Msa0129990 MtrunA17_Chr4g0042451 48.718 156 71 4 42 191 10 162 3.71e-39 140
Msa0129990 MtrunA17_Chr7g0254981 48.252 143 52 2 49 183 50 178 8.41e-39 137
Msa0129990 MtrunA17_Chr6g0461641 39.378 193 109 3 28 212 21 213 5.82e-38 138
Msa0129990 MtrunA17_Chr2g0321461 41.270 189 99 4 36 212 32 220 6.95e-38 138
Msa0129990 MtrunA17_Chr2g0291021 47.059 136 63 2 49 183 49 176 7.02e-33 123
Msa0129990 MtrunA17_Chr7g0261911 33.333 255 137 9 29 272 247 479 4.39e-32 124
Msa0129990 MtrunA17_Chr1g0190301 40.559 143 76 2 41 183 167 300 3.12e-28 112
Msa0129990 MtrunA17_Chr7g0274471 38.889 144 79 1 49 192 218 352 1.03e-27 111
Msa0129990 MtrunA17_Chr1g0175091 35.882 170 84 4 49 214 258 406 3.17e-26 107
Msa0129990 MtrunA17_Chr7g0258551 36.646 161 81 4 49 203 212 357 6.88e-26 105
Msa0129990 MtrunA17_Chr7g0242521 35.366 164 79 4 49 199 13 162 6.16e-20 86.7
Msa0129990 MtrunA17_Chr7g0243081 60.714 56 22 0 50 105 72 127 5.34e-17 80.1
Msa0129990 MtrunA17_Chr1g0175071 32.877 146 95 3 35 178 163 307 1.08e-16 79.0
Msa0129990 MtrunA17_Chr1g0175081 54.545 55 25 0 51 105 24 78 3.69e-16 72.8
Msa0129990 MtrunA17_Chr6g0479431 58.333 60 25 0 50 109 203 262 7.04e-16 77.0
Msa0129990 MtrunA17_Chr1g0154261 44.681 94 48 1 20 109 93 186 7.50e-16 76.3
Msa0129990 MtrunA17_Chr2g0288601 66.000 50 17 0 50 99 72 121 7.93e-16 72.8
Msa0129990 MtrunA17_Chr3g0095611 61.111 54 21 0 49 102 19 72 1.09e-15 74.3
Msa0129990 MtrunA17_Chr1g0210911 46.988 83 29 2 50 117 23 105 1.12e-15 76.3
Msa0129990 MtrunA17_Chr2g0320231 60.714 56 22 0 50 105 98 153 1.19e-15 76.6
Msa0129990 MtrunA17_Chr8g0379121 56.452 62 25 1 50 109 112 173 1.33e-15 76.6
Msa0129990 MtrunA17_Chr3g0109981 54.930 71 31 1 50 119 275 345 1.66e-15 76.6
Msa0129990 MtrunA17_Chr8g0366281 62.500 56 21 0 50 105 174 229 1.94e-15 75.9
Msa0129990 MtrunA17_Chr1g0162931 62.264 53 20 0 50 102 19 71 3.33e-15 74.3
Msa0129990 MtrunA17_Chr5g0410741 58.333 60 25 0 50 109 220 279 4.59e-15 75.1
Msa0129990 MtrunA17_Chr5g0402631 58.621 58 24 0 46 103 211 268 1.98e-13 69.7
Msa0129990 MtrunA17_Chr7g0258531 34.028 144 89 2 49 186 213 356 3.84e-13 68.9
Msa0129990 MtrunA17_Chr4g0031961 60.000 55 21 1 49 103 131 184 7.84e-13 67.8
Msa0129990 MtrunA17_Chr4g0067981 60.000 55 21 1 49 103 215 268 1.66e-12 67.8
Msa0129990 MtrunA17_Chr1g0161431 58.182 55 22 1 49 103 200 253 2.09e-12 67.4
Msa0129990 MtrunA17_Chr1g0198601 58.182 55 23 0 49 103 211 265 2.17e-12 67.0
Msa0129990 MtrunA17_Chr7g0255711 60.000 55 21 1 49 103 91 144 2.48e-12 66.2
Msa0129990 MtrunA17_Chr2g0283951 48.438 64 32 1 50 113 90 152 2.98e-12 66.6
Msa0129990 MtrunA17_Chr7g0275401 55.556 54 23 1 49 102 192 244 3.30e-12 65.9
Msa0129990 MtrunA17_Chr2g0294911 54.237 59 26 1 45 103 207 264 4.83e-12 66.2
Msa0129990 MtrunA17_Chr1g0149841 55.932 59 25 1 45 103 187 244 7.58e-12 65.5
Msa0129990 MtrunA17_Chr4g0062921 58.182 55 23 0 49 103 235 289 9.22e-12 65.1
Msa0129990 MtrunA17_Chr4g0009901 52.542 59 27 1 45 103 164 221 9.84e-12 64.3
Msa0129990 MtrunA17_Chr2g0304841 49.153 59 29 1 45 103 209 266 2.06e-11 64.3
Msa0129990 MtrunA17_Chr4g0045361 56.364 55 24 0 49 103 274 328 2.13e-11 63.9
Msa0129990 MtrunA17_Chr3g0137101 45.122 82 35 2 49 121 198 278 2.14e-11 64.3
Msa0129990 MtrunA17_Chr5g0422621 54.545 55 25 0 49 103 198 252 7.89e-11 62.0
Msa0129990 MtrunA17_Chr4g0009981 55.556 54 23 1 49 102 116 168 9.73e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0129990 AT3G24120.1 48.707 232 93 5 38 245 30 259 1.08e-64 205
Msa0129990 AT4G13640.1 48.889 225 95 4 39 244 27 250 9.58e-64 203
Msa0129990 AT3G24120.2 48.085 235 93 6 38 245 30 262 1.47e-62 200
Msa0129990 AT4G13640.2 48.246 228 95 5 39 244 27 253 1.37e-61 197
Msa0129990 AT1G79430.2 54.601 163 61 4 33 191 18 171 1.26e-47 163
Msa0129990 AT3G04030.3 39.754 244 111 8 29 246 25 258 1.59e-40 145
Msa0129990 AT3G12730.1 51.282 156 58 4 42 188 16 162 1.82e-40 141
Msa0129990 AT1G69580.1 47.337 169 68 4 38 191 23 185 3.05e-40 143
Msa0129990 AT1G69580.2 47.619 168 70 5 38 191 23 186 2.33e-39 141
Msa0129990 AT5G18240.1 37.190 242 120 6 29 246 25 258 2.01e-38 140
Msa0129990 AT5G18240.4 37.190 242 120 6 29 246 25 258 2.01e-38 140
Msa0129990 AT3G04030.2 38.934 244 112 9 29 246 25 257 1.35e-37 137
Msa0129990 AT5G18240.5 37.190 242 118 7 29 246 25 256 1.38e-36 135
Msa0129990 AT3G04030.1 38.525 244 108 9 29 246 25 252 5.52e-36 133
Msa0129990 AT5G45580.2 40.984 183 90 5 30 198 4 182 2.10e-35 129
Msa0129990 AT5G45580.1 43.529 170 78 5 43 198 1 166 3.53e-35 128
Msa0129990 AT2G01060.1 47.407 135 63 1 49 183 15 141 1.16e-34 127
Msa0129990 AT5G18240.2 35.950 242 117 7 29 246 25 252 6.01e-34 128
Msa0129990 AT5G18240.3 35.950 242 117 7 29 246 25 252 6.01e-34 128
Msa0129990 AT5G06800.2 41.481 135 74 1 49 183 192 321 1.03e-28 113
Msa0129990 AT3G13040.3 43.871 155 66 4 43 184 227 373 2.34e-28 113
Msa0129990 AT3G13040.1 43.871 155 66 4 43 184 227 373 2.34e-28 113
Msa0129990 AT3G13040.2 43.871 155 66 4 43 184 227 373 2.34e-28 113
Msa0129990 AT3G04450.4 39.241 158 81 4 36 189 187 333 2.34e-27 110
Msa0129990 AT3G04450.2 39.241 158 81 4 36 189 187 333 2.34e-27 110
Msa0129990 AT3G04450.1 39.241 158 81 4 36 189 227 373 3.63e-27 110
Msa0129990 AT5G06800.1 40.000 140 64 2 49 183 192 316 3.34e-26 106
Msa0129990 AT5G29000.1 38.356 146 80 2 49 192 188 325 2.02e-25 104
Msa0129990 AT5G29000.3 38.356 146 80 2 49 192 188 325 2.02e-25 104
Msa0129990 AT4G28610.1 40.441 136 71 2 49 183 225 351 2.28e-25 105
Msa0129990 AT5G29000.2 38.356 146 80 2 49 192 231 368 3.10e-25 104
Msa0129990 AT2G20400.2 39.706 136 72 2 49 183 231 357 2.54e-24 102
Msa0129990 AT2G06020.1 56.944 72 31 0 34 105 71 142 2.61e-24 100
Msa0129990 AT2G20400.1 39.259 135 73 1 49 183 231 356 1.49e-23 99.8
Msa0129990 AT1G79430.1 47.826 115 47 4 81 191 1 106 1.40e-20 90.1
Msa0129990 AT3G04450.3 48.276 87 39 2 36 122 227 307 4.44e-19 86.3
Msa0129990 AT5G06800.3 61.404 57 22 0 49 105 192 248 4.61e-19 85.5
Msa0129990 AT5G29000.4 59.649 57 23 0 49 105 231 287 5.21e-18 83.2
Msa0129990 AT4G04605.1 62.264 53 20 0 50 102 15 67 1.33e-17 80.1
Msa0129990 AT2G42660.1 62.500 56 21 0 50 105 51 106 5.88e-17 79.3
Msa0129990 AT3G04030.5 35.417 192 88 8 81 246 1 182 6.06e-17 80.1
Msa0129990 AT2G02060.2 61.017 59 23 0 48 106 28 86 7.43e-17 79.0
Msa0129990 AT2G02060.1 61.017 59 23 0 48 106 28 86 7.43e-17 79.0
Msa0129990 AT1G14600.1 63.636 55 20 0 48 102 22 76 1.03e-16 78.6
Msa0129990 AT2G38300.1 62.500 56 21 0 50 105 55 110 2.71e-16 78.6
Msa0129990 AT1G32240.2 50.617 81 40 0 50 130 214 294 4.36e-16 78.2
Msa0129990 AT1G32240.1 50.617 81 40 0 50 130 214 294 5.27e-16 78.2
Msa0129990 AT2G40260.1 60.714 56 22 0 50 105 83 138 1.75e-15 76.6
Msa0129990 AT5G16560.1 54.286 70 30 1 50 119 220 287 1.98e-15 76.6
Msa0129990 AT5G42630.2 58.333 60 25 0 50 109 106 165 2.67e-15 73.9
Msa0129990 AT5G42630.1 60.714 56 22 0 50 105 106 161 6.02e-15 73.9
Msa0129990 AT2G01060.2 40.000 100 52 1 84 183 1 92 1.18e-14 72.4
Msa0129990 AT4G17695.1 50.000 74 33 1 50 119 165 238 1.73e-14 73.2
Msa0129990 AT3G12730.2 41.026 117 51 5 81 188 1 108 6.42e-14 69.3
Msa0129990 AT5G59570.2 60.000 55 21 1 49 103 141 194 4.68e-13 68.6
Msa0129990 AT5G59570.1 60.000 55 21 1 49 103 141 194 4.68e-13 68.6
Msa0129990 AT4G04580.1 47.143 70 34 2 39 106 3 71 5.26e-13 66.2
Msa0129990 AT3G46640.2 60.000 55 21 1 49 103 144 197 5.69e-13 68.6
Msa0129990 AT3G46640.1 60.000 55 21 1 49 103 144 197 5.69e-13 68.6
Msa0129990 AT3G46640.3 60.000 55 21 1 49 103 144 197 6.66e-13 68.6
Msa0129990 AT4G37180.1 58.491 53 22 0 51 103 212 264 8.90e-13 68.6
Msa0129990 AT3G04030.4 33.854 192 85 9 81 246 1 176 9.21e-13 68.2
Msa0129990 AT4G37180.2 58.491 53 22 0 51 103 219 271 9.27e-13 68.6
Msa0129990 AT1G49560.1 60.000 55 22 0 49 103 192 246 1.10e-12 67.8
Msa0129990 AT3G10760.1 53.623 69 28 2 35 103 94 158 1.16e-12 67.8
Msa0129990 AT5G05090.1 60.000 55 21 1 49 103 81 134 1.87e-12 66.6
Msa0129990 AT3G16857.3 58.182 55 22 1 49 103 167 220 2.70e-12 67.4
Msa0129990 AT3G16857.2 58.182 55 22 1 49 103 237 290 2.95e-12 67.4
Msa0129990 AT3G16857.1 58.182 55 22 1 49 103 237 290 3.41e-12 67.4
Msa0129990 AT2G40970.1 60.000 55 21 1 49 103 105 158 4.05e-12 65.5
Msa0129990 AT3G25790.1 60.000 50 20 0 54 103 201 250 5.60e-12 65.9
Msa0129990 AT5G58080.1 46.914 81 34 3 49 121 194 273 5.72e-12 66.6
Msa0129990 AT4G04555.1 48.387 62 32 0 45 106 10 71 1.03e-11 61.2
Msa0129990 AT1G13300.1 56.364 55 24 0 49 103 181 235 1.96e-11 64.3
Msa0129990 AT2G03500.1 56.897 58 25 0 46 103 230 287 2.28e-11 64.3
Msa0129990 AT5G18240.6 30.000 190 95 7 81 246 1 176 4.64e-11 63.2
Msa0129990 AT5G44190.1 55.556 54 23 1 49 102 147 199 6.12e-11 63.2
Msa0129990 AT5G44190.2 55.556 54 23 1 49 102 147 199 7.35e-11 62.8
Msa0129990 AT1G25550.1 55.172 58 26 0 46 103 206 263 7.67e-11 62.4
Msa0129990 AT1G68670.1 56.364 55 24 0 49 103 215 269 9.08e-11 62.4

Find 93 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGAACAAGACTTTGGTTT+TGG 0.220050 1_3:-60059458 None:intergenic
AAATGCTTAGTAGCTTAATT+TGG 0.222202 1_3:+60063300 Msa0129990:three_prime_UTR
TTAATGCCTCCTTGACTTCA+TGG 0.229322 1_3:-60062052 None:intergenic
CTGTTTCACTCCTAGTTTGT+AGG 0.277225 1_3:+60063184 Msa0129990:three_prime_UTR
CATCAGAAGCTGGTAACTTT+GGG 0.286915 1_3:-60061917 None:intergenic
GTCCAACGAAGACGAGGTTT+TGG 0.346766 1_3:-60059758 None:intergenic
TCATTTAAAGAGTCATCTTC+AGG 0.349057 1_3:+60061477 Msa0129990:CDS
CCTTGAGGCTGGAGAATATA+TGG 0.350192 1_3:-60062930 None:intergenic
ATGGTCCTCATGATCGCTTT+TGG 0.371640 1_3:-60061430 None:intergenic
TAAGCTGATGGGAATGTAAC+TGG 0.371910 1_3:+60063249 Msa0129990:three_prime_UTR
CTATTCCTTGAGGATTGACT+TGG 0.373628 1_3:-60063118 None:intergenic
GCATCAGAAGCTGGTAACTT+TGG 0.381430 1_3:-60061918 None:intergenic
CAACAACAACACTTCCTTCA+TGG 0.381627 1_3:-60059652 None:intergenic
AGGAAGTGTTGTTGTTGTTC+AGG 0.385482 1_3:+60059658 Msa0129990:CDS
CTCAAAGGATTGACACTAGA+AGG 0.392959 1_3:+60062996 Msa0129990:CDS
TTGATGTATTGCAGAAGTAT+AGG 0.393167 1_3:+60061591 Msa0129990:intron
GACAAAGAGGATGCTGGGCT+TGG 0.410829 1_3:+60063031 Msa0129990:CDS
TCACCACTTTCTCTTCCAAA+TGG 0.412073 1_3:+60059486 Msa0129990:five_prime_UTR
AGATGCAGAACGGAGGTATA+TGG 0.412526 1_3:+60062773 Msa0129990:CDS
GGAAATGGAGATGACCCATT+TGG 0.413699 1_3:-60059501 None:intergenic
CACCACTTTCTCTTCCAAAT+GGG 0.414805 1_3:+60059487 Msa0129990:five_prime_UTR
ATGTTGGCTGATCAATTTAT+CGG 0.420599 1_3:+60062819 Msa0129990:CDS
TGTCAAACCCTTGACATTCA+TGG 0.421899 1_3:-60061449 None:intergenic
ACAGGAGAAAATGAAGGAAA+TGG 0.425402 1_3:-60059516 None:intergenic
AGTACCGAGCTAGGTGGAAA+TGG 0.432099 1_3:+60062888 Msa0129990:CDS
AACAGTTAGCCCCTTGAGGC+TGG 0.437173 1_3:-60062941 None:intergenic
GGAGGCATTAAGAGCACAAA+TGG 0.440107 1_3:+60062064 Msa0129990:CDS
CCATGGTATCACCTTTGATC+TGG 0.443004 1_3:+60063057 Msa0129990:CDS
TACTCGTTTGCATCAGAAGC+TGG 0.451451 1_3:-60061927 None:intergenic
TGAGACTCTCAAGTGATGTT+AGG 0.457932 1_3:-60062977 None:intergenic
AATAGCAAGGTATGGAATGT+AGG 0.459706 1_3:+60063136 Msa0129990:CDS
TTTAATTAATCCTACAAACT+AGG 0.484029 1_3:-60063194 None:intergenic
TATAGGCTTGGTAAGCAAGC+TGG 0.484630 1_3:+60061608 Msa0129990:CDS
GTTGATGCTGTCACTCAACT+TGG 0.487312 1_3:+60059803 Msa0129990:CDS
TTTGACGAGGGATGCAAAGA+TGG 0.489601 1_3:+60061638 Msa0129990:CDS
TATACCTCCGTTCTGCATCT+TGG 0.490483 1_3:-60062770 None:intergenic
TTTCCACCTAGCTCGGTACT+TGG 0.492435 1_3:-60062885 None:intergenic
TAGTGGGACAAAGAGGATGC+TGG 0.498265 1_3:+60063025 Msa0129990:CDS
CATATATTCTCCAGCCTCAA+GGG 0.500577 1_3:+60062931 Msa0129990:CDS
AAGTGAAAAGAACAAGACTT+TGG 0.504304 1_3:-60059464 None:intergenic
CCATATATTCTCCAGCCTCA+AGG 0.515023 1_3:+60062930 Msa0129990:CDS
CCTCAAGGAATAGCAAGGTA+TGG 0.523268 1_3:+60063128 Msa0129990:CDS
ATCCAAAACCTCGTCTTCGT+TGG 0.525904 1_3:+60059756 Msa0129990:CDS
CATTCCATTTCCACCTAGCT+CGG 0.529297 1_3:-60062892 None:intergenic
CCTTCATGGTCATGACTATG+AGG 0.529329 1_3:-60059638 None:intergenic
GACCCATTTGGAAGAGAAAG+TGG 0.533364 1_3:-60059489 None:intergenic
CCATACCTTGCTATTCCTTG+AGG 0.535847 1_3:-60063128 None:intergenic
AGTATTACAGGAGAAAATGA+AGG 0.543156 1_3:-60059522 None:intergenic
TGATGTTAGGCAACTTTCAG+TGG 0.544773 1_3:-60062964 None:intergenic
GATGCTGTCACTCAACTTGG+TGG 0.549084 1_3:+60059806 Msa0129990:CDS
CATCAGCTTAGATAAAAGAC+AGG 0.551223 1_3:-60063236 None:intergenic
GATGCTGGGCTTGGACTCCA+TGG 0.556025 1_3:+60063040 Msa0129990:CDS
GAACTACCAAGTACCGAGCT+AGG 0.561496 1_3:+60062879 Msa0129990:CDS
GAAACTAACATGAGAACTCA+AGG 0.562013 1_3:+60063083 Msa0129990:CDS
AGTGGGACAAAGAGGATGCT+GGG 0.566399 1_3:+60063026 Msa0129990:CDS
TATTCCTTGAGGATTGACTT+GGG 0.576040 1_3:-60063117 None:intergenic
AGTGAGAACAAGACAAGGGT+CGG 0.576442 1_3:-60059729 None:intergenic
TAGGTAGGACATTGTAAAGA+GGG 0.579450 1_3:+60063155 Msa0129990:exon
GTAGGTAGGACATTGTAAAG+AGG 0.582445 1_3:+60063154 Msa0129990:exon
TTAGTTTCACTCCAGATCAA+AGG 0.583008 1_3:-60063068 None:intergenic
ACATCACTTGAGAGTCTCAA+AGG 0.584426 1_3:+60062981 Msa0129990:CDS
AGCATCCTCTTTGTCCCACT+AGG 0.587029 1_3:-60063023 None:intergenic
CATGAGGACCATGAATGTCA+AGG 0.591196 1_3:+60061441 Msa0129990:CDS
TCATATCTTCTAGAAAGCCC+TGG 0.597094 1_3:+60061879 Msa0129990:CDS
ATATAGGGGCCATGAAGTCA+AGG 0.600134 1_3:+60062043 Msa0129990:intron
GCAAGGTATGGAATGTAGGT+AGG 0.601220 1_3:+60063140 Msa0129990:exon
ATCAGAAGCTGGTAACTTTG+GGG 0.602195 1_3:-60061916 None:intergenic
TAAGCTACATTTACTAGTGG+AGG 0.603652 1_3:+60062097 Msa0129990:CDS
ATGAGGACCATGAATGTCAA+GGG 0.610152 1_3:+60061442 Msa0129990:CDS
GTATTGCAGAAGTATAGGCT+TGG 0.612116 1_3:+60061596 Msa0129990:intron
GTGGAACAGTTAGCCCCTTG+AGG 0.612894 1_3:-60062945 None:intergenic
AAGTAAGCTACATTTACTAG+TGG 0.617641 1_3:+60062094 Msa0129990:CDS
CCACACCAAAAGCGATCATG+AGG 0.624758 1_3:+60061425 Msa0129990:CDS
TTAACCCAAGTCAATCCTCA+AGG 0.626775 1_3:+60063113 Msa0129990:CDS
TCAATCCTCAAGGAATAGCA+AGG 0.627368 1_3:+60063123 Msa0129990:CDS
CACTAGAAGGATCACCTAGT+GGG 0.627792 1_3:+60063009 Msa0129990:CDS
AGAGAGAGCGTGTAAGATGT+TGG 0.630995 1_3:+60062803 Msa0129990:CDS
GATCACCTAGTGGGACAAAG+AGG 0.632089 1_3:+60063018 Msa0129990:CDS
CATCAACAAAACGTTGGTGA+AGG 0.636840 1_3:-60059787 None:intergenic
CCTCATAGTCATGACCATGA+AGG 0.638338 1_3:+60059638 Msa0129990:CDS
ATATATTCTCCAGCCTCAAG+GGG 0.645538 1_3:+60062932 Msa0129990:CDS
CCAGATCAAAGGTGATACCA+TGG 0.651921 1_3:-60063057 None:intergenic
ATCACAGTAAAGCGTGACAG+TGG 0.652711 1_3:+60059415 Msa0129990:five_prime_UTR
TCAGAAGTGAGAACAAGACA+AGG 0.660603 1_3:-60059734 None:intergenic
CTTACACGCTCTCTCTAGCA+TGG 0.664799 1_3:-60062796 None:intergenic
TCAGTAGTCCAACGAAGACG+AGG 0.666354 1_3:-60059764 None:intergenic
AAATTCGCCAAGATGCAGAA+CGG 0.668751 1_3:+60062763 Msa0129990:CDS
ACACTAGAAGGATCACCTAG+TGG 0.682794 1_3:+60063008 Msa0129990:CDS
CTACCAAGTACCGAGCTAGG+TGG 0.694297 1_3:+60062882 Msa0129990:CDS
TGACAGCATCAACAAAACGT+TGG 0.700307 1_3:-60059793 None:intergenic
TTCGCCAAGATGCAGAACGG+AGG 0.705432 1_3:+60062766 Msa0129990:CDS
CAGAAGTGAGAACAAGACAA+GGG 0.729677 1_3:-60059733 None:intergenic
TAGGGGCCATGAAGTCAAGG+AGG 0.733476 1_3:+60062046 Msa0129990:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTGAAATTTTTTACT+AGG - chr1_3:60060667-60060686 None:intergenic 10.0%
!!! TTTTTTTTTTGTTGTTTTTT+TGG + chr1_3:60061851-60061870 Msa0129990:intron 10.0%
!!! TGAAATTTTTATAGTATTAC+AGG - chr1_3:60059537-60059556 None:intergenic 15.0%
!! ACAAAATTTAGTAAGAAAAA+TGG - chr1_3:60059859-60059878 None:intergenic 15.0%
!! AATGAATAAATTAACCAAAT+GGG - chr1_3:60059904-60059923 None:intergenic 15.0%
!! AAATGAATAAATTAACCAAA+TGG - chr1_3:60059905-60059924 None:intergenic 15.0%
!!! TAATCATTTTTATTTCATCT+TGG + chr1_3:60060103-60060122 Msa0129990:intron 15.0%
!! TAATTCACAAAACAAAAAAA+CGG - chr1_3:60060538-60060557 None:intergenic 15.0%
!!! TTAAGTGAAACTTTATATTT+TGG - chr1_3:60060768-60060787 None:intergenic 15.0%
!! CAAAATCATTCAAATTTAAT+TGG + chr1_3:60060838-60060857 Msa0129990:intron 15.0%
!!! TTATTGACTCAATATAATTA+AGG + chr1_3:60060902-60060921 Msa0129990:intron 15.0%
!!! TATTGACTCAATATAATTAA+GGG + chr1_3:60060903-60060922 Msa0129990:intron 15.0%
!!! AGATCATTGAATTTTATTAT+AGG + chr1_3:60061114-60061133 Msa0129990:intron 15.0%
!!! GATCATTGAATTTTATTATA+GGG + chr1_3:60061115-60061134 Msa0129990:intron 15.0%
!! AAACTACCATAAAAAATAAA+AGG - chr1_3:60061190-60061209 None:intergenic 15.0%
!! TATTATGATGTGAATAATAT+AGG + chr1_3:60061296-60061315 Msa0129990:intron 15.0%
!! ATTTATCAATTTGAATTACA+TGG + chr1_3:60061664-60061683 Msa0129990:intron 15.0%
!!! TTATTTTAGACCAATTTAAT+TGG + chr1_3:60062169-60062188 Msa0129990:intron 15.0%
!! AATATAACATTATAGAGTTA+GGG + chr1_3:60062232-60062251 Msa0129990:intron 15.0%
!! TGATTAATTATGAACTATTA+TGG + chr1_3:60062458-60062477 Msa0129990:intron 15.0%
!!! TTTCTTCTTTTTCTTTAACT+GGG + chr1_3:60059559-60059578 Msa0129990:five_prime_UTR 20.0%
!!! AACATGTTATTTTTCTTCTA+TGG - chr1_3:60059602-60059621 None:intergenic 20.0%
!!! AGAAAAATAACATGTTTTCT+AGG + chr1_3:60059606-60059625 Msa0129990:exon 20.0%
!!! ACTAAATTTTGTTACTTTTC+TGG + chr1_3:60059867-60059886 Msa0129990:intron 20.0%
!! ATGAATAAATTAACCAAATG+GGG - chr1_3:60059903-60059922 None:intergenic 20.0%
!! TATCAATGTCAAACTAAAAA+TGG - chr1_3:60059935-60059954 None:intergenic 20.0%
!!! ATAAATGGTTTTTGTTTTTG+AGG + chr1_3:60059958-60059977 Msa0129990:intron 20.0%
!! CAAATAAATCTATCTAAGAA+AGG - chr1_3:60060472-60060491 None:intergenic 20.0%
!! AATTCACAAAACAAAAAAAC+GGG - chr1_3:60060537-60060556 None:intergenic 20.0%
!! ACTTAAAATACAAAATCTCA+AGG + chr1_3:60060782-60060801 Msa0129990:intron 20.0%
!!! ACATTGCCTTTTATTTTTTA+TGG + chr1_3:60061181-60061200 Msa0129990:intron 20.0%
!! ATTGCACTAAATATTATAAC+TGG - chr1_3:60061530-60061549 None:intergenic 20.0%
!! TTTGTTTAATTATGTATCAC+TGG + chr1_3:60061714-60061733 Msa0129990:intron 20.0%
!!! TTTTTTGTTGTTTTTTTGGA+AGG + chr1_3:60061855-60061874 Msa0129990:intron 20.0%
!!! TTTTTGTTGTTTTTTTGGAA+GGG + chr1_3:60061856-60061875 Msa0129990:intron 20.0%
!!! ATTTAAGATGCCAATTAAAT+TGG - chr1_3:60062182-60062201 None:intergenic 20.0%
!! CAATATAACATTATAGAGTT+AGG + chr1_3:60062231-60062250 Msa0129990:intron 20.0%
!! GAAGTTAAGAATTATTATGT+TGG + chr1_3:60062658-60062677 Msa0129990:intron 20.0%
!! GTAGAAGTTAAGAATTATTA+TGG + chr1_3:60062716-60062735 Msa0129990:intron 20.0%
!! TTTAATTAATCCTACAAACT+AGG - chr1_3:60063197-60063216 None:intergenic 20.0%
!! CTTTCTTCTTTTTCTTTAAC+TGG + chr1_3:60059558-60059577 Msa0129990:five_prime_UTR 25.0%
! TTGACATTGATAGAGATAAA+TGG + chr1_3:60059943-60059962 Msa0129990:intron 25.0%
! TAGTGTAAATTATTGAAGCT+AGG + chr1_3:60059993-60060012 Msa0129990:intron 25.0%
! TAAATTATTGAAGCTAGGAA+AGG + chr1_3:60059998-60060017 Msa0129990:intron 25.0%
! TTTAAATTGTGTGAGATTTG+TGG + chr1_3:60060041-60060060 Msa0129990:intron 25.0%
! GTTTAACACAATTAGAATGT+GGG + chr1_3:60060284-60060303 Msa0129990:intron 25.0%
! TAAATCTATCTAAGAAAGGA+CGG - chr1_3:60060468-60060487 None:intergenic 25.0%
! ATTCACAAAACAAAAAAACG+GGG - chr1_3:60060536-60060555 None:intergenic 25.0%
!!! AACTTTATATTTTGGTTGGT+TGG - chr1_3:60060760-60060779 None:intergenic 25.0%
!!! GTGAAACTTTATATTTTGGT+TGG - chr1_3:60060764-60060783 None:intergenic 25.0%
! GTGACATTAGAATTTGAAAA+GGG - chr1_3:60060812-60060831 None:intergenic 25.0%
! AGTGACATTAGAATTTGAAA+AGG - chr1_3:60060813-60060832 None:intergenic 25.0%
!!! AATTGGTTTTCCTCTAAAAT+TGG + chr1_3:60060855-60060874 Msa0129990:intron 25.0%
! ATAATCCAAAGTCCAAATTA+GGG + chr1_3:60060938-60060957 Msa0129990:intron 25.0%
!! TCCAAATTAGGGTTTTTAAA+CGG + chr1_3:60060949-60060968 Msa0129990:intron 25.0%
!! TTATTTTCTCACTTTATGGT+GGG + chr1_3:60061215-60061234 Msa0129990:intron 25.0%
!!! TGTTTGTAATTTTAACCTGT+TGG + chr1_3:60061789-60061808 Msa0129990:intron 25.0%
!! TAAGAATTTCATTGTTTCTC+TGG + chr1_3:60061948-60061967 Msa0129990:intron 25.0%
!!! TTCGATCATTTTTTGACAAT+AGG - chr1_3:60062260-60062279 None:intergenic 25.0%
! GTTATATAGATTCTGTAGTA+TGG + chr1_3:60062314-60062333 Msa0129990:intron 25.0%
!!! TTTTTTTTTACCGTAGTTTG+TGG + chr1_3:60062595-60062614 Msa0129990:intron 25.0%
!!! AGAATTATTATGTTGGTAAC+TGG + chr1_3:60062665-60062684 Msa0129990:intron 25.0%
!! AGCGATTGAATTTTCTTATT+AGG + chr1_3:60062689-60062708 Msa0129990:intron 25.0%
! AAGAATTATTATGGTATTGC+AGG + chr1_3:60062725-60062744 Msa0129990:intron 25.0%
!!! TTGGTAGTTCTTGAAATTTT+TGG - chr1_3:60062869-60062888 None:intergenic 25.0%
! AAATGCTTAGTAGCTTAATT+TGG + chr1_3:60063300-60063319 Msa0129990:three_prime_UTR 25.0%
!!! AAGAACAAGACTTTGGTTTT+GGG - chr1_3:60059460-60059479 None:intergenic 30.0%
!! AAAGAACAAGACTTTGGTTT+TGG - chr1_3:60059461-60059480 None:intergenic 30.0%
AAGTGAAAAGAACAAGACTT+TGG - chr1_3:60059467-60059486 None:intergenic 30.0%
AGTATTACAGGAGAAAATGA+AGG - chr1_3:60059525-60059544 None:intergenic 30.0%
! TTCTTTTTCTTTAACTGGGT+TGG + chr1_3:60059563-60059582 Msa0129990:five_prime_UTR 30.0%
!! TGGTAAATTGCTGTTTGTTT+GGG + chr1_3:60060061-60060080 Msa0129990:intron 30.0%
TTGCGATTATGACAAATCTA+CGG + chr1_3:60060178-60060197 Msa0129990:intron 30.0%
TACCAAAAATGCACTTAAGA+AGG + chr1_3:60060204-60060223 Msa0129990:intron 30.0%
ACTAGTAAATACAACCTCAA+GGG + chr1_3:60060262-60060281 Msa0129990:intron 30.0%
GGTTTAACACAATTAGAATG+TGG + chr1_3:60060283-60060302 Msa0129990:intron 30.0%
CAAGTGCATAATTATTCACA+TGG - chr1_3:60060406-60060425 None:intergenic 30.0%
GATAGATTTATTTGTACCTC+TGG + chr1_3:60060478-60060497 Msa0129990:intron 30.0%
!!! TTGGTTTTTAGTGGAAAAAG+TGG - chr1_3:60060741-60060760 None:intergenic 30.0%
ACTTTGGATTATGACTGATT+TGG - chr1_3:60060930-60060949 None:intergenic 30.0%
CATAATCCAAAGTCCAAATT+AGG + chr1_3:60060937-60060956 Msa0129990:intron 30.0%
AAAAACCCTAATTTGGACTT+TGG - chr1_3:60060946-60060965 None:intergenic 30.0%
ACCGTTTAAAAACCCTAATT+TGG - chr1_3:60060953-60060972 None:intergenic 30.0%
TCATGCTAAGAAAATACCAA+AGG - chr1_3:60060979-60060998 None:intergenic 30.0%
! ATTTTCTTAGCATGATACCA+TGG + chr1_3:60060984-60061003 Msa0129990:intron 30.0%
AATACTAATAGCATTCTCCT+TGG - chr1_3:60061022-60061041 None:intergenic 30.0%
GATCTTCAGAAAAATTCTGA+AGG - chr1_3:60061099-60061118 None:intergenic 30.0%
!! ACGCTTATTTTCTCACTTTA+TGG + chr1_3:60061211-60061230 Msa0129990:intron 30.0%
!! CTTATTTTCTCACTTTATGG+TGG + chr1_3:60061214-60061233 Msa0129990:intron 30.0%
! TATTTTCTCACTTTATGGTG+GGG + chr1_3:60061216-60061235 Msa0129990:intron 30.0%
CCTCTCTAACTCTAATTATT+GGG + chr1_3:60061319-60061338 Msa0129990:intron 30.0%
CATTAGAAGAGTTTATCAGA+TGG - chr1_3:60061352-60061371 None:intergenic 30.0%
AACTCTTCTAATGCATCTAT+TGG + chr1_3:60061359-60061378 Msa0129990:intron 30.0%
TCATTTAAAGAGTCATCTTC+AGG + chr1_3:60061477-60061496 Msa0129990:CDS 30.0%
! TTGATGTATTGCAGAAGTAT+AGG + chr1_3:60061591-60061610 Msa0129990:intron 30.0%
! AAATCCTTTTCAATAGACTC+TGG + chr1_3:60061739-60061758 Msa0129990:intron 30.0%
! AAGTGTCATTCAGACATAAA+GGG - chr1_3:60061989-60062008 None:intergenic 30.0%
TGCAATCTCATGTCATATAT+AGG + chr1_3:60062027-60062046 Msa0129990:intron 30.0%
GCAATCTCATGTCATATATA+GGG + chr1_3:60062028-60062047 Msa0129990:intron 30.0%
CAATCTCATGTCATATATAG+GGG + chr1_3:60062029-60062048 Msa0129990:intron 30.0%
AAGTAAGCTACATTTACTAG+TGG + chr1_3:60062094-60062113 Msa0129990:CDS 30.0%
AAATGATCGAATCTCAACTT+AGG + chr1_3:60062269-60062288 Msa0129990:intron 30.0%
! TTTTTCTGTCTTTGGCAATT+GGG - chr1_3:60062362-60062381 None:intergenic 30.0%
TTGGTATTGTAAGTAGATTG+TGG + chr1_3:60062540-60062559 Msa0129990:intron 30.0%
TGGTATTGTAAGTAGATTGT+GGG + chr1_3:60062541-60062560 Msa0129990:intron 30.0%
! ATGTTGGCTGATCAATTTAT+CGG + chr1_3:60062819-60062838 Msa0129990:CDS 30.0%
! TGTCTTTTATCTAAGCTGAT+GGG + chr1_3:60063238-60063257 Msa0129990:three_prime_UTR 30.0%
ACAGGAGAAAATGAAGGAAA+TGG - chr1_3:60059519-60059538 None:intergenic 35.0%
GAAATGAGTTAGTTACTGGT+AGG - chr1_3:60059833-60059852 None:intergenic 35.0%
GAGAGAAATGAGTTAGTTAC+TGG - chr1_3:60059837-60059856 None:intergenic 35.0%
!! GTGGTAAATTGCTGTTTGTT+TGG + chr1_3:60060060-60060079 Msa0129990:intron 35.0%
TCTTGGATTTGTCAGAATCA+CGG + chr1_3:60060120-60060139 Msa0129990:intron 35.0%
! CACCTTCTTAAGTGCATTTT+TGG - chr1_3:60060209-60060228 None:intergenic 35.0%
CTTGAGGTTGTATTTACTAG+TGG - chr1_3:60060263-60060282 None:intergenic 35.0%
CACTAGTAAATACAACCTCA+AGG + chr1_3:60060261-60060280 Msa0129990:intron 35.0%
CTAATTGTGTTAAACCCTTG+AGG - chr1_3:60060279-60060298 None:intergenic 35.0%
TAACACAATTAGAATGTGGG+AGG + chr1_3:60060287-60060306 Msa0129990:intron 35.0%
GCTTACTATCCATGTACATA+TGG + chr1_3:60060440-60060459 Msa0129990:intron 35.0%
! TGGTAGCTAATGAGTGATTT+TGG + chr1_3:60060509-60060528 Msa0129990:intron 35.0%
ATGTCTTATCCCACTAAGTA+GGG - chr1_3:60060605-60060624 None:intergenic 35.0%
!!! TTTGGTTGGTTGGTTTTTAG+TGG - chr1_3:60060750-60060769 None:intergenic 35.0%
! AGCTGTTAGACCAATTTTAG+AGG - chr1_3:60060868-60060887 None:intergenic 35.0%
TTGAGTCAATAAAGTCTGTC+TGG - chr1_3:60060894-60060913 None:intergenic 35.0%
! GTTTTTAAACGGTACTCCTT+TGG + chr1_3:60060960-60060979 Msa0129990:intron 35.0%
! ATTTTCTCACTTTATGGTGG+GGG + chr1_3:60061217-60061236 Msa0129990:intron 35.0%
GCCTCTCTAACTCTAATTAT+TGG + chr1_3:60061318-60061337 Msa0129990:intron 35.0%
CCCAATAATTAGAGTTAGAG+AGG - chr1_3:60061322-60061341 None:intergenic 35.0%
TAGAAGAGTTTATCAGATGG+AGG - chr1_3:60061349-60061368 None:intergenic 35.0%
! AACCTGTTGGTTATTGTCTA+TGG + chr1_3:60061802-60061821 Msa0129990:intron 35.0%
! CAAGTGTCATTCAGACATAA+AGG - chr1_3:60061990-60062009 None:intergenic 35.0%
TGAGATTGCAATTCTCAGTT+CGG - chr1_3:60062017-60062036 None:intergenic 35.0%
TAAGCTACATTTACTAGTGG+AGG + chr1_3:60062097-60062116 Msa0129990:CDS 35.0%
TATGGAAAGTAGATCAGAGA+AGG + chr1_3:60062332-60062351 Msa0129990:intron 35.0%
! CTTTTTCTGTCTTTGGCAAT+TGG - chr1_3:60062363-60062382 None:intergenic 35.0%
CAATTGCCAAAGACAGAAAA+AGG + chr1_3:60062361-60062380 Msa0129990:intron 35.0%
! CATTTGCCTTTTTCTGTCTT+TGG - chr1_3:60062370-60062389 None:intergenic 35.0%
TGTGAAGAGTTAGAGGTTAA+TGG + chr1_3:60062422-60062441 Msa0129990:intron 35.0%
TCAATCATTGCTGTACAATC+AGG - chr1_3:60062567-60062586 None:intergenic 35.0%
TTAGTTTCACTCCAGATCAA+AGG - chr1_3:60063071-60063090 None:intergenic 35.0%
GAAACTAACATGAGAACTCA+AGG + chr1_3:60063083-60063102 Msa0129990:CDS 35.0%
! TATTCCTTGAGGATTGACTT+GGG - chr1_3:60063120-60063139 None:intergenic 35.0%
AATAGCAAGGTATGGAATGT+AGG + chr1_3:60063136-60063155 Msa0129990:CDS 35.0%
TAGGTAGGACATTGTAAAGA+GGG + chr1_3:60063155-60063174 Msa0129990:exon 35.0%
CATCAGCTTAGATAAAAGAC+AGG - chr1_3:60063239-60063258 None:intergenic 35.0%
! CTGTCTTTTATCTAAGCTGA+TGG + chr1_3:60063237-60063256 Msa0129990:three_prime_UTR 35.0%
TCACCACTTTCTCTTCCAAA+TGG + chr1_3:60059486-60059505 Msa0129990:five_prime_UTR 40.0%
CACCACTTTCTCTTCCAAAT+GGG + chr1_3:60059487-60059506 Msa0129990:five_prime_UTR 40.0%
CAACAACAACACTTCCTTCA+TGG - chr1_3:60059655-60059674 None:intergenic 40.0%
!! AGGAAGTGTTGTTGTTGTTC+AGG + chr1_3:60059658-60059677 Msa0129990:CDS 40.0%
CAGAAGTGAGAACAAGACAA+GGG - chr1_3:60059736-60059755 None:intergenic 40.0%
TCAGAAGTGAGAACAAGACA+AGG - chr1_3:60059737-60059756 None:intergenic 40.0%
! CATCAACAAAACGTTGGTGA+AGG - chr1_3:60059790-60059809 None:intergenic 40.0%
TGACAGCATCAACAAAACGT+TGG - chr1_3:60059796-60059815 None:intergenic 40.0%
GTGATGTTGTAAAAGCTACG+AGG - chr1_3:60060150-60060169 None:intergenic 40.0%
ATTAGAATGTGGGAGGTTTG+AGG + chr1_3:60060294-60060313 Msa0129990:intron 40.0%
CATGTCTTATCCCACTAAGT+AGG - chr1_3:60060606-60060625 None:intergenic 40.0%
CAGGGGACTTAATAAGATCA+AGG - chr1_3:60061050-60061069 None:intergenic 40.0%
TGATCTTATTAAGTCCCCTG+TGG + chr1_3:60061050-60061069 Msa0129990:intron 40.0%
GATCTTATTAAGTCCCCTGT+GGG + chr1_3:60061051-60061070 Msa0129990:intron 40.0%
TTCAGTCCTCATAGCTTTCT+TGG + chr1_3:60061139-60061158 Msa0129990:intron 40.0%
ATGAGGACCATGAATGTCAA+GGG + chr1_3:60061442-60061461 Msa0129990:CDS 40.0%
TGTCAAACCCTTGACATTCA+TGG - chr1_3:60061452-60061471 None:intergenic 40.0%
! GTATTGCAGAAGTATAGGCT+TGG + chr1_3:60061596-60061615 Msa0129990:intron 40.0%
!! AGCTGGAAAAGATTTTGACG+AGG + chr1_3:60061625-60061644 Msa0129990:CDS 40.0%
!! GCTGGAAAAGATTTTGACGA+GGG + chr1_3:60061626-60061645 Msa0129990:CDS 40.0%
GCAACCAGAGTCTATTGAAA+AGG - chr1_3:60061746-60061765 None:intergenic 40.0%
AGCCATAGACAATAACCAAC+AGG - chr1_3:60061807-60061826 None:intergenic 40.0%
! TCATATCTTCTAGAAAGCCC+TGG + chr1_3:60061879-60061898 Msa0129990:CDS 40.0%
ATCAGAAGCTGGTAACTTTG+GGG - chr1_3:60061919-60061938 None:intergenic 40.0%
CATCAGAAGCTGGTAACTTT+GGG - chr1_3:60061920-60061939 None:intergenic 40.0%
TTAATGCCTCCTTGACTTCA+TGG - chr1_3:60062055-60062074 None:intergenic 40.0%
TGATCGAATCTCAACTTAGG+TGG + chr1_3:60062272-60062291 Msa0129990:intron 40.0%
GAATCTCAACTTAGGTGGTT+TGG + chr1_3:60062277-60062296 Msa0129990:intron 40.0%
GTGAAACTGTGAAGAGTTAG+AGG + chr1_3:60062415-60062434 Msa0129990:intron 40.0%
! TGTTGTTTGATCCGTGTATC+CGG + chr1_3:60062481-60062500 Msa0129990:intron 40.0%
CCAAGCATTATCCACTTGAT+GGG - chr1_3:60062507-60062526 None:intergenic 40.0%
!!! GCTTGGCTGTTTTTGTTGAT+TGG + chr1_3:60062521-60062540 Msa0129990:intron 40.0%
GAAGTCTATGCCACAAACTA+CGG - chr1_3:60062608-60062627 None:intergenic 40.0%
AAATTCGCCAAGATGCAGAA+CGG + chr1_3:60062763-60062782 Msa0129990:CDS 40.0%
CATATATTCTCCAGCCTCAA+GGG + chr1_3:60062931-60062950 Msa0129990:CDS 40.0%
ATATATTCTCCAGCCTCAAG+GGG + chr1_3:60062932-60062951 Msa0129990:CDS 40.0%
TGATGTTAGGCAACTTTCAG+TGG - chr1_3:60062967-60062986 None:intergenic 40.0%
TGAGACTCTCAAGTGATGTT+AGG - chr1_3:60062980-60062999 None:intergenic 40.0%
ACATCACTTGAGAGTCTCAA+AGG + chr1_3:60062981-60063000 Msa0129990:CDS 40.0%
CTCAAAGGATTGACACTAGA+AGG + chr1_3:60062996-60063015 Msa0129990:CDS 40.0%
TTAACCCAAGTCAATCCTCA+AGG + chr1_3:60063113-60063132 Msa0129990:CDS 40.0%
CTATTCCTTGAGGATTGACT+TGG - chr1_3:60063121-60063140 None:intergenic 40.0%
TCAATCCTCAAGGAATAGCA+AGG + chr1_3:60063123-60063142 Msa0129990:CDS 40.0%
GTAGGTAGGACATTGTAAAG+AGG + chr1_3:60063154-60063173 Msa0129990:exon 40.0%
! CTGTTTCACTCCTAGTTTGT+AGG + chr1_3:60063184-60063203 Msa0129990:three_prime_UTR 40.0%
TAAGCTGATGGGAATGTAAC+TGG + chr1_3:60063249-60063268 Msa0129990:three_prime_UTR 40.0%
ATCACAGTAAAGCGTGACAG+TGG + chr1_3:60059415-60059434 Msa0129990:five_prime_UTR 45.0%
GACCCATTTGGAAGAGAAAG+TGG - chr1_3:60059492-60059511 None:intergenic 45.0%
GGAAATGGAGATGACCCATT+TGG - chr1_3:60059504-60059523 None:intergenic 45.0%
CCTTCATGGTCATGACTATG+AGG - chr1_3:60059641-60059660 None:intergenic 45.0%
CCTCATAGTCATGACCATGA+AGG + chr1_3:60059638-60059657 Msa0129990:CDS 45.0%
AGTGAGAACAAGACAAGGGT+CGG - chr1_3:60059732-60059751 None:intergenic 45.0%
ATCCAAAACCTCGTCTTCGT+TGG + chr1_3:60059756-60059775 Msa0129990:CDS 45.0%
GTTGATGCTGTCACTCAACT+TGG + chr1_3:60059803-60059822 Msa0129990:CDS 45.0%
!!! TGGTTTTGATGCACCCCATT+TGG + chr1_3:60059887-60059906 Msa0129990:intron 45.0%
AGGACGGTACCATATGTACA+TGG - chr1_3:60060452-60060471 None:intergenic 45.0%
TTGTACCTCTGGTCTCTCTA+TGG + chr1_3:60060489-60060508 Msa0129990:intron 45.0%
TATAGCCGACCCTACTTAGT+GGG + chr1_3:60060593-60060612 Msa0129990:intron 45.0%
CTTATCCCACTAAGTAGGGT+CGG - chr1_3:60060601-60060620 None:intergenic 45.0%
GCTCAACCAAGAAAGCTATG+AGG - chr1_3:60061148-60061167 None:intergenic 45.0%
! ATGGTCCTCATGATCGCTTT+TGG - chr1_3:60061433-60061452 None:intergenic 45.0%
CATGAGGACCATGAATGTCA+AGG + chr1_3:60061441-60061460 Msa0129990:CDS 45.0%
! TATAGGCTTGGTAAGCAAGC+TGG + chr1_3:60061608-60061627 Msa0129990:CDS 45.0%
TTTGACGAGGGATGCAAAGA+TGG + chr1_3:60061638-60061657 Msa0129990:CDS 45.0%
! GGGATGAGTTTTCAGTACCA+GGG - chr1_3:60061899-60061918 None:intergenic 45.0%
GCATCAGAAGCTGGTAACTT+TGG - chr1_3:60061921-60061940 None:intergenic 45.0%
TACTCGTTTGCATCAGAAGC+TGG - chr1_3:60061930-60061949 None:intergenic 45.0%
ATATAGGGGCCATGAAGTCA+AGG + chr1_3:60062043-60062062 Msa0129990:intron 45.0%
!! GGAGGCATTAAGAGCACAAA+TGG + chr1_3:60062064-60062083 Msa0129990:CDS 45.0%
! TTAGGTGGTTTGGACATGTG+TGG + chr1_3:60062287-60062306 Msa0129990:intron 45.0%
CCCATCAAGTGGATAATGCT+TGG + chr1_3:60062504-60062523 Msa0129990:intron 45.0%
GCCAAGCATTATCCACTTGA+TGG - chr1_3:60062508-60062527 None:intergenic 45.0%
TATACCTCCGTTCTGCATCT+TGG - chr1_3:60062773-60062792 None:intergenic 45.0%
! AGATGCAGAACGGAGGTATA+TGG + chr1_3:60062773-60062792 Msa0129990:CDS 45.0%
! AGAGAGAGCGTGTAAGATGT+TGG + chr1_3:60062803-60062822 Msa0129990:CDS 45.0%
CATTCCATTTCCACCTAGCT+CGG - chr1_3:60062895-60062914 None:intergenic 45.0%
CCTTGAGGCTGGAGAATATA+TGG - chr1_3:60062933-60062952 None:intergenic 45.0%
CCATATATTCTCCAGCCTCA+AGG + chr1_3:60062930-60062949 Msa0129990:CDS 45.0%
ACACTAGAAGGATCACCTAG+TGG + chr1_3:60063008-60063027 Msa0129990:CDS 45.0%
CACTAGAAGGATCACCTAGT+GGG + chr1_3:60063009-60063028 Msa0129990:CDS 45.0%
CCAGATCAAAGGTGATACCA+TGG - chr1_3:60063060-60063079 None:intergenic 45.0%
CCATGGTATCACCTTTGATC+TGG + chr1_3:60063057-60063076 Msa0129990:CDS 45.0%
!! CCATACCTTGCTATTCCTTG+AGG - chr1_3:60063131-60063150 None:intergenic 45.0%
CCTCAAGGAATAGCAAGGTA+TGG + chr1_3:60063128-60063147 Msa0129990:CDS 45.0%
GCAAGGTATGGAATGTAGGT+AGG + chr1_3:60063140-60063159 Msa0129990:exon 45.0%
! GTCCAACGAAGACGAGGTTT+TGG - chr1_3:60059761-60059780 None:intergenic 50.0%
TCAGTAGTCCAACGAAGACG+AGG - chr1_3:60059767-60059786 None:intergenic 50.0%
GATGCTGTCACTCAACTTGG+TGG + chr1_3:60059806-60059825 Msa0129990:CDS 50.0%
AGCTACCATAGAGAGACCAG+AGG - chr1_3:60060497-60060516 None:intergenic 50.0%
CTATAGCCGACCCTACTTAG+TGG + chr1_3:60060592-60060611 Msa0129990:intron 50.0%
CTTGGCTCAATCGTAGACCA+TGG - chr1_3:60061004-60061023 None:intergenic 50.0%
CATGGTCTACGATTGAGCCA+AGG + chr1_3:60061002-60061021 Msa0129990:intron 50.0%
ATTAAGTCCCCTGTGGGAGA+AGG + chr1_3:60061057-60061076 Msa0129990:intron 50.0%
TTAAGTCCCCTGTGGGAGAA+GGG + chr1_3:60061058-60061077 Msa0129990:intron 50.0%
TGAAGAACCCTTCTCCCACA+GGG - chr1_3:60061068-60061087 None:intergenic 50.0%
ATGAAGAACCCTTCTCCCAC+AGG - chr1_3:60061069-60061088 None:intergenic 50.0%
!! CCTCATGATCGCTTTTGGTG+TGG - chr1_3:60061428-60061447 None:intergenic 50.0%
CCACACCAAAAGCGATCATG+AGG + chr1_3:60061425-60061444 Msa0129990:CDS 50.0%
! GGGGATGAGTTTTCAGTACC+AGG - chr1_3:60061900-60061919 None:intergenic 50.0%
! GCATTATCCACTTGATGGGC+CGG - chr1_3:60062503-60062522 None:intergenic 50.0%
CTTACACGCTCTCTCTAGCA+TGG - chr1_3:60062799-60062818 None:intergenic 50.0%
!! GAACTACCAAGTACCGAGCT+AGG + chr1_3:60062879-60062898 Msa0129990:CDS 50.0%
TTTCCACCTAGCTCGGTACT+TGG - chr1_3:60062888-60062907 None:intergenic 50.0%
! AGTACCGAGCTAGGTGGAAA+TGG + chr1_3:60062888-60062907 Msa0129990:CDS 50.0%
GATCACCTAGTGGGACAAAG+AGG + chr1_3:60063018-60063037 Msa0129990:CDS 50.0%
AGCATCCTCTTTGTCCCACT+AGG - chr1_3:60063026-60063045 None:intergenic 50.0%
TAGTGGGACAAAGAGGATGC+TGG + chr1_3:60063025-60063044 Msa0129990:CDS 50.0%
AGTGGGACAAAGAGGATGCT+GGG + chr1_3:60063026-60063045 Msa0129990:CDS 50.0%
GAAGAACCCTTCTCCCACAG+GGG - chr1_3:60061067-60061086 None:intergenic 55.0%
TAGGGGCCATGAAGTCAAGG+AGG + chr1_3:60062046-60062065 Msa0129990:intron 55.0%
! GGTGGTTTGGACATGTGTGG+AGG + chr1_3:60062290-60062309 Msa0129990:intron 55.0%
! CACTTGATGGGCCGGATACA+CGG - chr1_3:60062495-60062514 None:intergenic 55.0%
TTCGCCAAGATGCAGAACGG+AGG + chr1_3:60062766-60062785 Msa0129990:CDS 55.0%
!! CTACCAAGTACCGAGCTAGG+TGG + chr1_3:60062882-60062901 Msa0129990:CDS 55.0%
AACAGTTAGCCCCTTGAGGC+TGG - chr1_3:60062944-60062963 None:intergenic 55.0%
GTGGAACAGTTAGCCCCTTG+AGG - chr1_3:60062948-60062967 None:intergenic 55.0%
GACAAAGAGGATGCTGGGCT+TGG + chr1_3:60063031-60063050 Msa0129990:CDS 55.0%
CGTGTATCCGGCCCATCAAG+TGG + chr1_3:60062493-60062512 Msa0129990:intron 60.0%
GATGCTGGGCTTGGACTCCA+TGG + chr1_3:60063040-60063059 Msa0129990:CDS 60.0%
CGGCAGCAGCAGCAGCAGCA+AGG - chr1_3:60059712-60059731 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 60059401 60063345 60059401 ID=Msa0129990;Name=Msa0129990
chr1_3 mRNA 60059401 60063345 60059401 ID=Msa0129990-mRNA-1;Parent=Msa0129990;Name=Msa0129990-mRNA-1;_AED=0.05;_eAED=0.05;_QI=216|1|1|1|1|1|6|188|300
chr1_3 exon 60059401 60059836 60059401 ID=Msa0129990-mRNA-1:exon:19667;Parent=Msa0129990-mRNA-1
chr1_3 exon 60061422 60061498 60061422 ID=Msa0129990-mRNA-1:exon:19668;Parent=Msa0129990-mRNA-1
chr1_3 exon 60061605 60061659 60061605 ID=Msa0129990-mRNA-1:exon:19669;Parent=Msa0129990-mRNA-1
chr1_3 exon 60061877 60061946 60061877 ID=Msa0129990-mRNA-1:exon:19670;Parent=Msa0129990-mRNA-1
chr1_3 exon 60062049 60062118 60062049 ID=Msa0129990-mRNA-1:exon:19671;Parent=Msa0129990-mRNA-1
chr1_3 exon 60062747 60063345 60062747 ID=Msa0129990-mRNA-1:exon:19672;Parent=Msa0129990-mRNA-1
chr1_3 five_prime_UTR 60059401 60059616 60059401 ID=Msa0129990-mRNA-1:five_prime_utr;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60059617 60059836 60059617 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60061422 60061498 60061422 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60061605 60061659 60061605 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60061877 60061946 60061877 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60062049 60062118 60062049 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 CDS 60062747 60063157 60062747 ID=Msa0129990-mRNA-1:cds;Parent=Msa0129990-mRNA-1
chr1_3 three_prime_UTR 60063158 60063345 60063158 ID=Msa0129990-mRNA-1:three_prime_utr;Parent=Msa0129990-mRNA-1
Gene Sequence

>Msa0129990

ATGTTTTCTAGGTTGATACGTCCTCATAGTCATGACCATGAAGGAAGTGTTGTTGTTGTTCAGGAAGATACAAATCATCATCTTCACACTCACCTTGCTGCTGCTGCTGCTGCCGACCCTTGTCTTGTTCTCACTTCTGATCCAAAACCTCGTCTTCGTTGGACTACTGACCTTCACCAACGTTTTGTTGATGCTGTCACTCAACTTGGTGGTCCTACCAAAGCCACACCAAAAGCGATCATGAGGACCATGAATGTCAAGGGTTTGACACTCTATCATTTAAAGAGTCATCTTCAGAAGTATAGGCTTGGTAAGCAAGCTGGAAAAGATTTTGACGAGGGATGCAAAGATGGGTCATATCTTCTAGAAAGCCCTGGTACTGAAAACTCATCCCCAAAGTTACCAGCTTCTGATGCAAACGAGGGCCATGAAGTCAAGGAGGCATTAAGAGCACAAATGGAAGTGCAAAGTAAGCTACATTTACTAGTGGAGGCAGAGAAGCACTTGCAAATTCGCCAAGATGCAGAACGGAGGTATATGGCCATGCTAGAGAGAGCGTGTAAGATGTTGGCTGATCAATTTATCGGTGATACAACTATAGACACAGACATCCAAAAATTTCAAGAACTACCAAGTACCGAGCTAGGTGGAAATGGAATGCATGTTTCAGAAATACCATATATTCTCCAGCCTCAAGGGGCTAACTGTTCCACTGAAAGTTGCCTAACATCACTTGAGAGTCTCAAAGGATTGACACTAGAAGGATCACCTAGTGGGACAAAGAGGATGCTGGGCTTGGACTCCATGGTATCACCTTTGATCTGGAGTGAAACTAACATGAGAACTCAAGGCATTCATTTAACCCAAGTCAATCCTCAAGGAATAGCAAGGTATGGAATGTAG
Protein sequence

>Msa0129990

MFSRLIRPHSHDHEGSVVVVQEDTNHHLHTHLAAAAAADPCLVLTSDPKPRLRWTTDLHQRFVDAVTQLGGPTKATPKAIMRTMNVKGLTLYHLKSHLQKYRLGKQAGKDFDEGCKDGSYLLESPGTENSSPKLPASDANEGHEVKEALRAQMEVQSKLHLLVEAEKHLQIRQDAERRYMAMLERACKMLADQFIGDTTIDTDIQKFQELPSTELGGNGMHVSEIPYILQPQGANCSTESCLTSLESLKGLTLEGSPSGTKRMLGLDSMVSPLIWSETNMRTQGIHLTQVNPQGIARYGM*