AlfalfaGEDB | Msa0162240

M. sativa cultivar zhongmu-4 / Msa0162240

KEGG: -
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0008144 GO:0016020 GO:0016208 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0036094 GO:0043167 GO:0043168 GO:0043169 GO:0044464 GO:0071944 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00582.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0162240	XP_013467750.1	97.895	190	4	0	1	190	1	190	1.41e-135	387

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0162240	sp\|Q8LGG8\|USPAL_ARATH	25.595	168	104	3	23	181	7	162	3.83e-16	75.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0162240	A0A072VJ06	97.895	190	4	0	1	190	1	190	6.72e-136	387

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0023000	Msa0162240	0.830619	2.652611e-55	-8.615850e-47
Msa0070880	Msa0162240	0.847211	1.334915e-59	-8.615850e-47
Msa0118780	Msa0162240	0.842472	2.532376e-58	-8.615850e-47
Msa0162100	Msa0162240	0.874760	5.247840e-68	-8.615850e-47
Msa0162220	Msa0162240	0.903852	2.259119e-79	-8.615850e-47
Msa0162230	Msa0162240	0.999769	0.000000e+00	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0162240	MtrunA17_Chr1g0176311	97.895	190	4	0	1	190	1	190	1.29e-139	387
Msa0162240	MtrunA17_Chr1g0176321	80.226	177	29	4	20	190	1	177	5.15e-99	283
Msa0162240	MtrunA17_Chr1g0192651	40.741	162	83	3	23	184	10	158	2.22e-34	119
Msa0162240	MtrunA17_Chr1g0195661	37.989	179	93	5	13	187	3	167	4.30e-32	113
Msa0162240	MtrunA17_Chr1g0194801	35.294	170	94	5	17	183	2	158	6.68e-31	110
Msa0162240	MtrunA17_Chr5g0427291	35.976	164	96	4	24	181	10	170	1.36e-27	102
Msa0162240	MtrunA17_Chr7g0269301	33.929	168	98	3	21	187	7	162	1.92e-23	91.3
Msa0162240	MtrunA17_Chr4g0001641	49.180	61	31	0	121	181	5	65	6.13e-19	77.0
Msa0162240	MtrunA17_Chr4g0032261	32.738	168	88	4	23	181	6	157	9.37e-19	79.0
Msa0162240	MtrunA17_Chr2g0294421	31.138	167	104	3	23	187	6	163	1.51e-18	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0162240	AT3G11930.1	56.886	167	70	2	23	188	34	199	6.52e-69	209
Msa0162240	AT3G11930.2	57.143	168	69	3	23	188	34	200	7.87e-68	206
Msa0162240	AT3G11930.4	56.805	169	69	3	23	188	34	201	1.03e-67	206
Msa0162240	AT3G11930.3	49.485	194	69	4	23	188	34	226	3.38e-61	190
Msa0162240	AT3G58450.1	53.704	162	74	1	24	185	32	192	4.24e-59	184
Msa0162240	AT3G58450.2	53.086	162	68	3	24	185	32	185	2.99e-55	174
Msa0162240	AT1G09740.2	39.024	164	93	2	24	185	10	168	3.49e-31	111
Msa0162240	AT1G09740.1	39.024	164	93	2	24	185	10	168	3.49e-31	111
Msa0162240	AT3G62550.1	34.731	167	95	4	17	181	2	156	3.49e-26	98.6
Msa0162240	AT2G47710.1	33.333	165	97	3	21	184	7	159	4.37e-26	98.6

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Find 55 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGAAGAGATTATCAA+GGG	0.605580	1_4:+38748063	None:intergenic
AACTTTATGACTGCCATGGA+AGG	0.559729	1_4:-38748046	Msa0162240:CDS
AAGCTTCTATGCTCTTAAGT+GGG	0.645712	1_4:-38748086	Msa0162240:CDS
AAGGATGAAGATGAAGGTGA+TGG	0.645453	1_4:-38748131	Msa0162240:CDS
AATAGGTAGAGCTATTGATG+AGG	0.611252	1_4:-38748176	Msa0162240:CDS
AATCATGGGTAGCCGTGGCC+TGG	0.369904	1_4:-38746339	Msa0162240:CDS
AATTCTCAATGGAGACCCAA+GGG	0.664861	1_4:-38746405	Msa0162240:CDS
AGAGAGAAGGATGAAGATGA+AGG	0.511016	1_4:-38748137	Msa0162240:CDS
AGATACGAACCAGCACCACC+TGG	0.633023	1_4:+38747944	None:intergenic
AGGAACATCATCTGAAAATG+AGG	0.493972	1_4:-38748026	Msa0162240:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCACATAATTGTGGAAAAT+GGG	+	chr1_4:38745530-38745549	None:intergenic	30.0%
ATTAAATGGATCACTTCAAG+TGG	-	chr1_4:38746078-38746097	Msa0162240:intron	30.0%
AGAAAATAAGGGAATAGCTT+TGG	+	chr1_4:38746266-38746285	None:intergenic	30.0%
GGGTGTAATTGAAGAAAATA+AGG	+	chr1_4:38746278-38746297	None:intergenic	30.0%
CTTTGACCAAGAAAAACAAA+GGG	+	chr1_4:38746298-38746317	None:intergenic	30.0%
TAGATCAATTACCCATTATC+TGG	+	chr1_4:38746321-38746340	None:intergenic	30.0%
AATGGGTAATTGATCTACAA+AGG	-	chr1_4:38746324-38746343	Msa0162240:intron	30.0%
ATGGGTAATTGATCTACAAA+GGG	-	chr1_4:38746325-38746344	Msa0162240:intron	30.0%
GGGATGAATCAATTTAGAAA+AGG	+	chr1_4:38746712-38746731	None:intergenic	30.0%
TAGGTGTTCATTTCTTAAGT+GGG	+	chr1_4:38746732-38746751	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	38745320	38748206	38745320	ID=Msa0162240;Name=Msa0162240
chr1_4	mRNA	38745320	38748206	38745320	ID=Msa0162240-mRNA-1;Parent=Msa0162240;Name=Msa0162240-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|4\|0\|190
chr1_4	exon	38747954	38748206	38747954	ID=Msa0162240-mRNA-1:exon:11322;Parent=Msa0162240-mRNA-1
chr1_4	exon	38747579	38747682	38747579	ID=Msa0162240-mRNA-1:exon:11321;Parent=Msa0162240-mRNA-1
chr1_4	exon	38746326	38746444	38746326	ID=Msa0162240-mRNA-1:exon:11320;Parent=Msa0162240-mRNA-1
chr1_4	exon	38745320	38745416	38745320	ID=Msa0162240-mRNA-1:exon:11319;Parent=Msa0162240-mRNA-1
chr1_4	CDS	38747954	38748206	38747954	ID=Msa0162240-mRNA-1:cds;Parent=Msa0162240-mRNA-1
chr1_4	CDS	38747579	38747682	38747579	ID=Msa0162240-mRNA-1:cds;Parent=Msa0162240-mRNA-1
chr1_4	CDS	38746326	38746444	38746326	ID=Msa0162240-mRNA-1:cds;Parent=Msa0162240-mRNA-1
chr1_4	CDS	38745320	38745416	38745320	ID=Msa0162240-mRNA-1:cds;Parent=Msa0162240-mRNA-1

Gene Sequence

>Msa0162240

ATGGAAGAAATAGGTAGAGCTATTGATGAGGGGAGGGAAGGTGCAGAAGAGAGAAGGATGAAGATGAAGGTGATGGTGGCAATAGATGAGAGTGATGGAAGCTTCTATGCTCTTAAGTGGGCCCTTGATAATCTCTTCAACTTTATGACTGCCATGGAAGGAACATCATCTGAAAATGAGGGAATGGTGTTTCTTGTTCATGTTGAACCCATTTTCCACAATTATGTGCACCCTATTGGACCAGGTGGTGCTGCTTTTTATCCAGCATCTGTGGTTGTGGATTCAGTGAAGAAAGGCCAGCAAGAAAGATCAGCAGTTATTCTTTCGCGGGCTTTGCAAATGTGCAAGGATAAGCAGGTCAAAGCAGAAAGTGTAATTCTCAATGGAGACCCAAGGGAAATGATTTGTCAGGCTTCAGAACAAATGCAAGTTGATCTCTTAATCATGGGTAGCCGTGGCCTGGGCACACTCAAAAGAGCATTTCTTGGAAGTGTAAGCGACTATTGTGCACATCATGCAAAAGCACCTATTCTCATTGTGAAGCCACCAGAGGAGCACCATAAAAAGCATTGA

Protein sequence

>Msa0162240

MEEIGRAIDEGREGAEERRMKMKVMVAIDESDGSFYALKWALDNLFNFMTAMEGTSSENEGMVFLVHVEPIFHNYVHPIGPGGAAFYPASVVVDSVKKGQQERSAVILSRALQMCKDKQVKAESVILNGDPREMICQASEQMQVDLLIMGSRGLGTLKRAFLGSVSDYCAHHAKAPILIVKPPEEHHKKH*