AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0172270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172270 MtrunA17_Chr1g0190961 99.270 137 1 0 1 137 1 137 1.05e-95 271
Msa0172270 MtrunA17_Chr7g0273831 85.401 137 19 1 1 137 1 136 1.20e-79 231
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0172270 AT5G28060.1 86.364 132 18 0 1 132 1 132 1.90e-83 241
Msa0172270 AT3G04920.1 89.394 132 14 0 1 132 1 132 4.72e-76 222
Msa0172270 AT3G04920.2 84.466 103 15 1 1 102 1 103 5.00e-52 160

Find 49 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGTTGGTCATGAACTTTC+TGG 0.100542 1_4:+57232922 None:intergenic
TTCAAGTTCAGAACTCATTT+TGG 0.214352 1_4:-57232385 Msa0172270:CDS
TTTAACAGAATTCTCAAAAT+TGG 0.248098 1_4:+57230978 None:intergenic
TTGGAAACATTTGCCCTTCC+AGG 0.258852 1_4:+57232611 None:intergenic
GGCGGCAAGTCCACTGGTTT+TGG 0.272758 1_4:-57232361 Msa0172270:CDS
ACCCTAACAAGTCTGTATTT+TGG 0.279167 1_4:+57232298 None:intergenic
TTGCTGTTGGTAGTTTCTTA+AGG 0.335656 1_4:-57231113 Msa0172270:three_prime_UTR
GAAACTCACGAATTGCTTCC+TGG 0.336108 1_4:+57232892 None:intergenic
AACCAAAATACAGACTTGTT+AGG 0.354436 1_4:-57232300 Msa0172270:CDS
CTAACAATCCACAATCAAAA+AGG 0.377651 1_4:+57230908 None:intergenic
AAATGAAGGAAAGAAAGAAC+AGG 0.391792 1_4:-57231333 Msa0172270:CDS
CTTGGCAGCATCACCAGCCT+TGG 0.412083 1_4:+57231189 None:intergenic
GCTGGTGATGCTGCCAAGGC+TGG 0.425056 1_4:-57231184 Msa0172270:CDS
GTCATTGATGTTCTTCATCC+TGG 0.431746 1_4:-57232629 Msa0172270:CDS
CAACACTATAACCATGTAAA+CGG 0.433061 1_4:+57230852 None:intergenic
AACAACAACAATGGCAGACA+AGG 0.443820 1_4:-57232960 Msa0172270:exon
TGCTTCAATTCTCTCATCTC+AGG 0.447361 1_4:-57233009 Msa0172270:five_prime_UTR
TTCTCAAAATTGGTCACTGC+TGG 0.474339 1_4:+57230988 None:intergenic
TTATTTCTTCTTGCCAGCCT+TGG 0.476966 1_4:+57231171 None:intergenic
TGACCAACAGGCTTCTCTCC+AGG 0.487847 1_4:-57232910 Msa0172270:CDS
CCAGAAAGTTCATGACCAAC+AGG 0.493715 1_4:-57232922 Msa0172270:CDS
GTAGAATGGACTTGATACTA+AGG 0.495750 1_4:-57231374 Msa0172270:intron
CAAGGCTGGTGATGCTGCCA+AGG 0.500785 1_4:-57231188 Msa0172270:CDS
TTTGGAGGCGGCAAGTCCAC+TGG 0.503274 1_4:-57232367 Msa0172270:CDS
GCTCTATAGAACCGTTTACA+TGG 0.506580 1_4:-57230863 Msa0172270:three_prime_UTR
TGGTGTGAAGGTCGAGCTAA+AGG 0.510706 1_4:-57232458 Msa0172270:intron
ACCAAAATACAGACTTGTTA+GGG 0.511637 1_4:-57232299 Msa0172270:intron
GAAGATCCGTGGAGTTAAGA+AGG 0.518538 1_4:-57231305 Msa0172270:intron
ATTGAAGCAGCTCTGAGAGT+TGG 0.523143 1_4:+57233023 None:intergenic
TCTCATCTCAGGTAGACAAC+CGG 0.528063 1_4:-57232998 Msa0172270:five_prime_UTR
CATGAACTTTCTGGTTCTGA+TGG 0.533482 1_4:+57232931 None:intergenic
GCTCTGAGAGTTGGAACGAA+GGG 0.534348 1_4:+57233032 None:intergenic
TGTTGTTGAGTTGCGGCCGC+CGG 0.540908 1_4:+57232979 None:intergenic
TTGATGTTCTTCATCCTGGA+AGG 0.545793 1_4:-57232625 Msa0172270:CDS
ATACTAAGGTTGAAAAGTCA+AGG 0.557383 1_4:-57231360 Msa0172270:CDS
TTTGGACAAGATCAAACAGT+TGG 0.560959 1_4:+57230793 None:intergenic
GCTTTACCTTCTTAACTCCA+CGG 0.569745 1_4:+57231299 None:intergenic
CTTTCTGGTTCTGATGGTAA+CGG 0.571013 1_4:+57232937 None:intergenic
TGCAAGGATTTACGATGTGA+AGG 0.571848 1_4:-57232428 Msa0172270:CDS
AAAGTCAAGGAAGCAAATGA+AGG 0.574321 1_4:-57231347 Msa0172270:CDS
CTTCCTGGAGAGAAGCCTGT+TGG 0.575264 1_4:+57232907 None:intergenic
AGCTAAAGGAGAAGCTTGCA+AGG 0.579868 1_4:-57232444 Msa0172270:CDS
TGATGTTCTTCATCCTGGAA+GGG 0.593002 1_4:-57232624 Msa0172270:CDS
AGCTCTGAGAGTTGGAACGA+AGG 0.595174 1_4:+57233031 None:intergenic
TGGAAGGGCAAATGTTTCCA+AGG 0.598113 1_4:-57232609 Msa0172270:intron
AACAGGGCAAAGAAGATCCG+TGG 0.612698 1_4:-57231316 Msa0172270:CDS
ATAGATCAAACCAAAACCAG+TGG 0.662445 1_4:+57232351 None:intergenic
AATGAAGGAAAGAAAGAACA+GGG 0.674567 1_4:-57231332 Msa0172270:CDS
CATCTCAGGTAGACAACCGG+CGG 0.767907 1_4:-57232995 Msa0172270:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTATGTCGAATTATTTTT+AGG - chr1_4:57231159-57231178 Msa0172270:exon 15.0%
!! CTTGAAAAAAATAAATGAAT+AGG + chr1_4:57231915-57231934 None:intergenic 15.0%
!! AATGATTGAAATTAAATGTA+GGG + chr1_4:57232070-57232089 None:intergenic 15.0%
!! AAATGATTGAAATTAAATGT+AGG + chr1_4:57232071-57232090 None:intergenic 15.0%
!!! TATAATAGTTTTTTCTAGAA+TGG - chr1_4:57232770-57232789 Msa0172270:intron 15.0%
!!! ATTTATGTCGAATTATTTTT+AGG - chr1_4:57231159-57231178 Msa0172270:exon 15.0%
!! CTTGAAAAAAATAAATGAAT+AGG + chr1_4:57231915-57231934 None:intergenic 15.0%
!! AATGATTGAAATTAAATGTA+GGG + chr1_4:57232070-57232089 None:intergenic 15.0%
!! AAATGATTGAAATTAAATGT+AGG + chr1_4:57232071-57232090 None:intergenic 15.0%
!!! TATAATAGTTTTTTCTAGAA+TGG - chr1_4:57232770-57232789 Msa0172270:intron 15.0%
!! ATACAGATCTAATAAAGAAA+GGG + chr1_4:57230986-57231005 None:intergenic 20.0%
!! TATACAGATCTAATAAAGAA+AGG + chr1_4:57230987-57231006 None:intergenic 20.0%
!! TAGTATGATTAGAATTAGTA+TGG - chr1_4:57231133-57231152 Msa0172270:three_prime_UTR 20.0%
!! TCATCACACAATAAAAAAAT+CGG + chr1_4:57231195-57231214 None:intergenic 20.0%
!!! ATTTTTTTATTGTGTGATGA+AGG - chr1_4:57231195-57231214 Msa0172270:intron 20.0%
!! ACAAATAAACCACAAAAAAA+AGG + chr1_4:57231620-57231639 None:intergenic 20.0%
!! AAAAAACAAACAAATCCATT+TGG + chr1_4:57231754-57231773 None:intergenic 20.0%
!!! AATGGATTTGTTTGTTTTTT+CGG - chr1_4:57231754-57231773 Msa0172270:intron 20.0%
!!! CTTTATTTTCTTATGTAGTT+TGG - chr1_4:57232124-57232143 Msa0172270:intron 20.0%
!!! TTTTCTTTTCTATTGTAGAA+TGG - chr1_4:57232458-57232477 Msa0172270:intron 20.0%
!! TTTAACAGAATTCTCAAAAT+TGG + chr1_4:57232871-57232890 None:intergenic 20.0%
!! ATACAGATCTAATAAAGAAA+GGG + chr1_4:57230986-57231005 None:intergenic 20.0%
!! TATACAGATCTAATAAAGAA+AGG + chr1_4:57230987-57231006 None:intergenic 20.0%
!! TAGTATGATTAGAATTAGTA+TGG - chr1_4:57231133-57231152 Msa0172270:three_prime_UTR 20.0%
!! TCATCACACAATAAAAAAAT+CGG + chr1_4:57231195-57231214 None:intergenic 20.0%
!!! ATTTTTTTATTGTGTGATGA+AGG - chr1_4:57231195-57231214 Msa0172270:intron 20.0%
!! ACAAATAAACCACAAAAAAA+AGG + chr1_4:57231620-57231639 None:intergenic 20.0%
!! AAAAAACAAACAAATCCATT+TGG + chr1_4:57231754-57231773 None:intergenic 20.0%
!!! AATGGATTTGTTTGTTTTTT+CGG - chr1_4:57231754-57231773 Msa0172270:intron 20.0%
!!! CTTTATTTTCTTATGTAGTT+TGG - chr1_4:57232124-57232143 Msa0172270:intron 20.0%
!!! TTTTCTTTTCTATTGTAGAA+TGG - chr1_4:57232458-57232477 Msa0172270:intron 20.0%
!! TTTAACAGAATTCTCAAAAT+TGG + chr1_4:57232871-57232890 None:intergenic 20.0%
! GATACAAATAACAAATACCT+TGG + chr1_4:57231257-57231276 None:intergenic 25.0%
! ATATGCTATGAATGTTAGAT+AGG - chr1_4:57231279-57231298 Msa0172270:intron 25.0%
! CTGAAACTTACGTTATATTA+TGG - chr1_4:57231329-57231348 Msa0172270:CDS 25.0%
! TACGTTATATTATGGTGTAT+TGG - chr1_4:57231337-57231356 Msa0172270:CDS 25.0%
!!! TTGTTTGTTTTTTCGGAATT+TGG - chr1_4:57231761-57231780 Msa0172270:intron 25.0%
!! TATTTCTGTTAAATAGTTCC+TGG - chr1_4:57232001-57232020 Msa0172270:intron 25.0%
!! CAAGAGAGTACATAATTATA+GGG + chr1_4:57232175-57232194 None:intergenic 25.0%
!! TCAAGAGAGTACATAATTAT+AGG + chr1_4:57232176-57232195 None:intergenic 25.0%
!!! GTCATTTTGTTATGATATCA+AGG - chr1_4:57232705-57232724 Msa0172270:intron 25.0%
!!! AATAGTTTTTTCTAGAATGG+AGG - chr1_4:57232773-57232792 Msa0172270:intron 25.0%
! GATACAAATAACAAATACCT+TGG + chr1_4:57231257-57231276 None:intergenic 25.0%
! ATATGCTATGAATGTTAGAT+AGG - chr1_4:57231279-57231298 Msa0172270:intron 25.0%
! CTGAAACTTACGTTATATTA+TGG - chr1_4:57231329-57231348 Msa0172270:CDS 25.0%
! TACGTTATATTATGGTGTAT+TGG - chr1_4:57231337-57231356 Msa0172270:CDS 25.0%
!!! TTGTTTGTTTTTTCGGAATT+TGG - chr1_4:57231761-57231780 Msa0172270:intron 25.0%
!! TATTTCTGTTAAATAGTTCC+TGG - chr1_4:57232001-57232020 Msa0172270:intron 25.0%
!! CAAGAGAGTACATAATTATA+GGG + chr1_4:57232175-57232194 None:intergenic 25.0%
!! TCAAGAGAGTACATAATTAT+AGG + chr1_4:57232176-57232195 None:intergenic 25.0%
!!! GTCATTTTGTTATGATATCA+AGG - chr1_4:57232705-57232724 Msa0172270:intron 25.0%
!!! AATAGTTTTTTCTAGAATGG+AGG - chr1_4:57232773-57232792 Msa0172270:intron 25.0%
TCTAATAAAGAAAGGGAAGA+AGG + chr1_4:57230979-57230998 None:intergenic 30.0%
GATGATTTATATTGTCTCAG+AGG - chr1_4:57231306-57231325 Msa0172270:CDS 30.0%
!! GATTTATCGTTTGTTGTGTT+TGG - chr1_4:57231368-57231387 Msa0172270:CDS 30.0%
TGAACACAAAAACAGTGTTT+GGG + chr1_4:57231445-57231464 None:intergenic 30.0%
TTGAACACAAAAACAGTGTT+TGG + chr1_4:57231446-57231465 None:intergenic 30.0%
!! TTCAAGTTCAGAACTCATTT+TGG - chr1_4:57231461-57231480 Msa0172270:intron 30.0%
!!! GTATTTTGGTTCATACTTCT+TGG + chr1_4:57231537-57231556 None:intergenic 30.0%
! AACCAAAATACAGACTTGTT+AGG - chr1_4:57231546-57231565 Msa0172270:intron 30.0%
!! ACCAAAATACAGACTTGTTA+GGG - chr1_4:57231547-57231566 Msa0172270:intron 30.0%
! GTTAATTCTGTCTTTTCTCT+TGG - chr1_4:57231573-57231592 Msa0172270:intron 30.0%
! TGTCTTTTCTCTTGGATAAT+TGG - chr1_4:57231581-57231600 Msa0172270:intron 30.0%
!!! GCTTTGTAACCTTTTTTTTG+TGG - chr1_4:57231608-57231627 Msa0172270:intron 30.0%
!! TGATTTAGTTTGCTATTTCC+TGG - chr1_4:57231689-57231708 Msa0172270:intron 30.0%
ACTAAATTCACAAGCAAATG+AGG + chr1_4:57231881-57231900 None:intergenic 30.0%
GATTGAAATTAAATGTAGGG+TGG + chr1_4:57232067-57232086 None:intergenic 30.0%
TAGTTTGGAGATAATTTAGC+TGG - chr1_4:57232139-57232158 Msa0172270:intron 30.0%
! TTTGCTTGTAGTGTTGTTAT+TGG - chr1_4:57232275-57232294 Msa0172270:intron 30.0%
!!! ACTTTTCTGAAATTTGCCAA+GGG - chr1_4:57232344-57232363 Msa0172270:CDS 30.0%
GATTCATCAACTACATTAAC+AGG + chr1_4:57232379-57232398 None:intergenic 30.0%
ATACTAAGGTTGAAAAGTCA+AGG - chr1_4:57232486-57232505 Msa0172270:intron 30.0%
AAATGAAGGAAAGAAAGAAC+AGG - chr1_4:57232513-57232532 Msa0172270:intron 30.0%
AATGAAGGAAAGAAAGAACA+GGG - chr1_4:57232514-57232533 Msa0172270:intron 30.0%
!! TATCAAGGTTATTTTGCTGT+TGG - chr1_4:57232720-57232739 Msa0172270:intron 30.0%
!! CTTAATCTCCTTTTTGATTG+TGG - chr1_4:57232930-57232949 Msa0172270:CDS 30.0%
CTAACAATCCACAATCAAAA+AGG + chr1_4:57232941-57232960 None:intergenic 30.0%
CAACACTATAACCATGTAAA+CGG + chr1_4:57232997-57233016 None:intergenic 30.0%
TCTAATAAAGAAAGGGAAGA+AGG + chr1_4:57230979-57230998 None:intergenic 30.0%
GATGATTTATATTGTCTCAG+AGG - chr1_4:57231306-57231325 Msa0172270:CDS 30.0%
!! GATTTATCGTTTGTTGTGTT+TGG - chr1_4:57231368-57231387 Msa0172270:CDS 30.0%
TGAACACAAAAACAGTGTTT+GGG + chr1_4:57231445-57231464 None:intergenic 30.0%
TTGAACACAAAAACAGTGTT+TGG + chr1_4:57231446-57231465 None:intergenic 30.0%
!! TTCAAGTTCAGAACTCATTT+TGG - chr1_4:57231461-57231480 Msa0172270:intron 30.0%
!!! GTATTTTGGTTCATACTTCT+TGG + chr1_4:57231537-57231556 None:intergenic 30.0%
! AACCAAAATACAGACTTGTT+AGG - chr1_4:57231546-57231565 Msa0172270:intron 30.0%
!! ACCAAAATACAGACTTGTTA+GGG - chr1_4:57231547-57231566 Msa0172270:intron 30.0%
! GTTAATTCTGTCTTTTCTCT+TGG - chr1_4:57231573-57231592 Msa0172270:intron 30.0%
! TGTCTTTTCTCTTGGATAAT+TGG - chr1_4:57231581-57231600 Msa0172270:intron 30.0%
!!! GCTTTGTAACCTTTTTTTTG+TGG - chr1_4:57231608-57231627 Msa0172270:intron 30.0%
!! TGATTTAGTTTGCTATTTCC+TGG - chr1_4:57231689-57231708 Msa0172270:intron 30.0%
ACTAAATTCACAAGCAAATG+AGG + chr1_4:57231881-57231900 None:intergenic 30.0%
GATTGAAATTAAATGTAGGG+TGG + chr1_4:57232067-57232086 None:intergenic 30.0%
TAGTTTGGAGATAATTTAGC+TGG - chr1_4:57232139-57232158 Msa0172270:intron 30.0%
! TTTGCTTGTAGTGTTGTTAT+TGG - chr1_4:57232275-57232294 Msa0172270:intron 30.0%
!!! ACTTTTCTGAAATTTGCCAA+GGG - chr1_4:57232344-57232363 Msa0172270:CDS 30.0%
GATTCATCAACTACATTAAC+AGG + chr1_4:57232379-57232398 None:intergenic 30.0%
ATACTAAGGTTGAAAAGTCA+AGG - chr1_4:57232486-57232505 Msa0172270:intron 30.0%
AAATGAAGGAAAGAAAGAAC+AGG - chr1_4:57232513-57232532 Msa0172270:intron 30.0%
AATGAAGGAAAGAAAGAACA+GGG - chr1_4:57232514-57232533 Msa0172270:intron 30.0%
!! TATCAAGGTTATTTTGCTGT+TGG - chr1_4:57232720-57232739 Msa0172270:intron 30.0%
!! CTTAATCTCCTTTTTGATTG+TGG - chr1_4:57232930-57232949 Msa0172270:CDS 30.0%
CTAACAATCCACAATCAAAA+AGG + chr1_4:57232941-57232960 None:intergenic 30.0%
CAACACTATAACCATGTAAA+CGG + chr1_4:57232997-57233016 None:intergenic 30.0%
! TTGTTGTTGTTGTTGAGTTG+CGG + chr1_4:57230877-57230896 None:intergenic 35.0%
CAACTCAACAACAACAACAA+TGG - chr1_4:57230877-57230896 Msa0172270:three_prime_UTR 35.0%
! AAGTTCAGAACTCATTTTGG+AGG - chr1_4:57231464-57231483 Msa0172270:intron 35.0%
ATAGATCAAACCAAAACCAG+TGG + chr1_4:57231498-57231517 None:intergenic 35.0%
! ACCCTAACAAGTCTGTATTT+TGG + chr1_4:57231551-57231570 None:intergenic 35.0%
TTTCTCTTGGATAATTGGAC+TGG - chr1_4:57231586-57231605 Msa0172270:intron 35.0%
CAACATCAATGTTAACAACC+AGG + chr1_4:57231710-57231729 None:intergenic 35.0%
CTGGTTGTTAACATTGATGT+TGG - chr1_4:57231708-57231727 Msa0172270:intron 35.0%
!! ACATCAGTGTTTTTGCCAAA+TGG - chr1_4:57231736-57231755 Msa0172270:intron 35.0%
!!! TGCACTTCCTTTTGATTTGA+CGG - chr1_4:57231961-57231980 Msa0172270:intron 35.0%
! CGTGTTTGTTAACAAGTGAA+GGG - chr1_4:57232036-57232055 Msa0172270:intron 35.0%
ACTCTCTTGAAGAATTTGTC+CGG - chr1_4:57232186-57232205 Msa0172270:intron 35.0%
TAGTTATTTGTATTGCCTGC+TGG - chr1_4:57232301-57232320 Msa0172270:CDS 35.0%
AGTTATTTGTATTGCCTGCT+GGG - chr1_4:57232302-57232321 Msa0172270:CDS 35.0%
!!! CACTTTTCTGAAATTTGCCA+AGG - chr1_4:57232343-57232362 Msa0172270:CDS 35.0%
CAACTACATTAACAGGCAAA+AGG + chr1_4:57232372-57232391 None:intergenic 35.0%
! GTAGAATGGACTTGATACTA+AGG - chr1_4:57232472-57232491 Msa0172270:intron 35.0%
AAAGTCAAGGAAGCAAATGA+AGG - chr1_4:57232499-57232518 Msa0172270:intron 35.0%
!! CATTGTATTTTTGCAGACCA+AGG - chr1_4:57232640-57232659 Msa0172270:intron 35.0%
!! TTGCTGTTGGTAGTTTCTTA+AGG - chr1_4:57232733-57232752 Msa0172270:intron 35.0%
! TTTGAGATCAACCAACTGTT+TGG - chr1_4:57233042-57233061 Msa0172270:five_prime_UTR 35.0%
! TTGTTGTTGTTGTTGAGTTG+CGG + chr1_4:57230877-57230896 None:intergenic 35.0%
CAACTCAACAACAACAACAA+TGG - chr1_4:57230877-57230896 Msa0172270:three_prime_UTR 35.0%
! AAGTTCAGAACTCATTTTGG+AGG - chr1_4:57231464-57231483 Msa0172270:intron 35.0%
ATAGATCAAACCAAAACCAG+TGG + chr1_4:57231498-57231517 None:intergenic 35.0%
! ACCCTAACAAGTCTGTATTT+TGG + chr1_4:57231551-57231570 None:intergenic 35.0%
TTTCTCTTGGATAATTGGAC+TGG - chr1_4:57231586-57231605 Msa0172270:intron 35.0%
CAACATCAATGTTAACAACC+AGG + chr1_4:57231710-57231729 None:intergenic 35.0%
CTGGTTGTTAACATTGATGT+TGG - chr1_4:57231708-57231727 Msa0172270:intron 35.0%
!! ACATCAGTGTTTTTGCCAAA+TGG - chr1_4:57231736-57231755 Msa0172270:intron 35.0%
!!! TGCACTTCCTTTTGATTTGA+CGG - chr1_4:57231961-57231980 Msa0172270:intron 35.0%
! CGTGTTTGTTAACAAGTGAA+GGG - chr1_4:57232036-57232055 Msa0172270:intron 35.0%
ACTCTCTTGAAGAATTTGTC+CGG - chr1_4:57232186-57232205 Msa0172270:intron 35.0%
TAGTTATTTGTATTGCCTGC+TGG - chr1_4:57232301-57232320 Msa0172270:CDS 35.0%
AGTTATTTGTATTGCCTGCT+GGG - chr1_4:57232302-57232321 Msa0172270:CDS 35.0%
!!! CACTTTTCTGAAATTTGCCA+AGG - chr1_4:57232343-57232362 Msa0172270:CDS 35.0%
CAACTACATTAACAGGCAAA+AGG + chr1_4:57232372-57232391 None:intergenic 35.0%
! GTAGAATGGACTTGATACTA+AGG - chr1_4:57232472-57232491 Msa0172270:intron 35.0%
AAAGTCAAGGAAGCAAATGA+AGG - chr1_4:57232499-57232518 Msa0172270:intron 35.0%
!! CATTGTATTTTTGCAGACCA+AGG - chr1_4:57232640-57232659 Msa0172270:intron 35.0%
!! TTGCTGTTGGTAGTTTCTTA+AGG - chr1_4:57232733-57232752 Msa0172270:intron 35.0%
TGCTTCAATTCTCTCATCTC+AGG - chr1_4:57230837-57230856 Msa0172270:three_prime_UTR 40.0%
AACAACAACAATGGCAGACA+AGG - chr1_4:57230886-57230905 Msa0172270:three_prime_UTR 40.0%
!! CTTTCTGGTTCTGATGGTAA+CGG + chr1_4:57230912-57230931 None:intergenic 40.0%
!! CATGAACTTTCTGGTTCTGA+TGG + chr1_4:57230918-57230937 None:intergenic 40.0%
GTCATTGATGTTCTTCATCC+TGG - chr1_4:57231217-57231236 Msa0172270:intron 40.0%
TTGATGTTCTTCATCCTGGA+AGG - chr1_4:57231221-57231240 Msa0172270:intron 40.0%
TGATGTTCTTCATCCTGGAA+GGG - chr1_4:57231222-57231241 Msa0172270:intron 40.0%
!! GTTTGTTGTGTTTGGTGTGA+AGG - chr1_4:57231376-57231395 Msa0172270:intron 40.0%
TGCAAGGATTTACGATGTGA+AGG - chr1_4:57231418-57231437 Msa0172270:intron 40.0%
!! TTCAGAACTCATTTTGGAGG+CGG - chr1_4:57231467-57231486 Msa0172270:intron 40.0%
TGGCAAAAACACTGATGTTG+AGG + chr1_4:57231734-57231753 None:intergenic 40.0%
! GGAAGTGCAAAGTCAAACAT+CGG + chr1_4:57231950-57231969 None:intergenic 40.0%
!!! GCACTTCCTTTTGATTTGAC+GGG - chr1_4:57231962-57231981 Msa0172270:intron 40.0%
! GCGTGTTTGTTAACAAGTGA+AGG - chr1_4:57232035-57232054 Msa0172270:intron 40.0%
ACAAATTCAAACGTGAGCAC+CGG + chr1_4:57232208-57232227 None:intergenic 40.0%
TCACGTTTGAATTTGTCCAG+TGG - chr1_4:57232212-57232231 Msa0172270:intron 40.0%
GCTTTACCTTCTTAACTCCA+CGG + chr1_4:57232550-57232569 None:intergenic 40.0%
! TTATTTCTTCTTGCCAGCCT+TGG + chr1_4:57232678-57232697 None:intergenic 40.0%
TTCTCAAAATTGGTCACTGC+TGG + chr1_4:57232861-57232880 None:intergenic 40.0%
GCTCTATAGAACCGTTTACA+TGG - chr1_4:57232983-57233002 Msa0172270:five_prime_UTR 40.0%
TGCTTCAATTCTCTCATCTC+AGG - chr1_4:57230837-57230856 Msa0172270:three_prime_UTR 40.0%
AACAACAACAATGGCAGACA+AGG - chr1_4:57230886-57230905 Msa0172270:three_prime_UTR 40.0%
!! CTTTCTGGTTCTGATGGTAA+CGG + chr1_4:57230912-57230931 None:intergenic 40.0%
!! CATGAACTTTCTGGTTCTGA+TGG + chr1_4:57230918-57230937 None:intergenic 40.0%
GTCATTGATGTTCTTCATCC+TGG - chr1_4:57231217-57231236 Msa0172270:intron 40.0%
TTGATGTTCTTCATCCTGGA+AGG - chr1_4:57231221-57231240 Msa0172270:intron 40.0%
TGATGTTCTTCATCCTGGAA+GGG - chr1_4:57231222-57231241 Msa0172270:intron 40.0%
!! GTTTGTTGTGTTTGGTGTGA+AGG - chr1_4:57231376-57231395 Msa0172270:intron 40.0%
TGCAAGGATTTACGATGTGA+AGG - chr1_4:57231418-57231437 Msa0172270:intron 40.0%
!! TTCAGAACTCATTTTGGAGG+CGG - chr1_4:57231467-57231486 Msa0172270:intron 40.0%
TGGCAAAAACACTGATGTTG+AGG + chr1_4:57231734-57231753 None:intergenic 40.0%
! GGAAGTGCAAAGTCAAACAT+CGG + chr1_4:57231950-57231969 None:intergenic 40.0%
!!! GCACTTCCTTTTGATTTGAC+GGG - chr1_4:57231962-57231981 Msa0172270:intron 40.0%
! GCGTGTTTGTTAACAAGTGA+AGG - chr1_4:57232035-57232054 Msa0172270:intron 40.0%
ACAAATTCAAACGTGAGCAC+CGG + chr1_4:57232208-57232227 None:intergenic 40.0%
TCACGTTTGAATTTGTCCAG+TGG - chr1_4:57232212-57232231 Msa0172270:intron 40.0%
GCTTTACCTTCTTAACTCCA+CGG + chr1_4:57232550-57232569 None:intergenic 40.0%
! TTATTTCTTCTTGCCAGCCT+TGG + chr1_4:57232678-57232697 None:intergenic 40.0%
TTCTCAAAATTGGTCACTGC+TGG + chr1_4:57232861-57232880 None:intergenic 40.0%
GCTCTATAGAACCGTTTACA+TGG - chr1_4:57232983-57233002 Msa0172270:five_prime_UTR 40.0%
!! ATTGAAGCAGCTCTGAGAGT+TGG + chr1_4:57230826-57230845 None:intergenic 45.0%
TCTCATCTCAGGTAGACAAC+CGG - chr1_4:57230848-57230867 Msa0172270:three_prime_UTR 45.0%
CCTGTTGGTCATGAACTTTC+TGG + chr1_4:57230927-57230946 None:intergenic 45.0%
CCAGAAAGTTCATGACCAAC+AGG - chr1_4:57230924-57230943 Msa0172270:three_prime_UTR 45.0%
GAAACTCACGAATTGCTTCC+TGG + chr1_4:57230957-57230976 None:intergenic 45.0%
TTGGAAACATTTGCCCTTCC+AGG + chr1_4:57231238-57231257 None:intergenic 45.0%
TGGAAGGGCAAATGTTTCCA+AGG - chr1_4:57231237-57231256 Msa0172270:intron 45.0%
AGCTAAAGGAGAAGCTTGCA+AGG - chr1_4:57231402-57231421 Msa0172270:intron 45.0%
TACGCTCCCGTCAAATCAAA+AGG + chr1_4:57231971-57231990 None:intergenic 45.0%
! TGATTTGACGGGAGCGTATT+TGG - chr1_4:57231973-57231992 Msa0172270:intron 45.0%
AACACGCTTATAACAGCACC+AGG + chr1_4:57232022-57232041 None:intergenic 45.0%
GAAGATCCGTGGAGTTAAGA+AGG - chr1_4:57232541-57232560 Msa0172270:intron 45.0%
!! GTATTTTTGCAGACCAAGGC+TGG - chr1_4:57232644-57232663 Msa0172270:intron 45.0%
!! ATTGAAGCAGCTCTGAGAGT+TGG + chr1_4:57230826-57230845 None:intergenic 45.0%
TCTCATCTCAGGTAGACAAC+CGG - chr1_4:57230848-57230867 Msa0172270:three_prime_UTR 45.0%
CCTGTTGGTCATGAACTTTC+TGG + chr1_4:57230927-57230946 None:intergenic 45.0%
CCAGAAAGTTCATGACCAAC+AGG - chr1_4:57230924-57230943 Msa0172270:three_prime_UTR 45.0%
GAAACTCACGAATTGCTTCC+TGG + chr1_4:57230957-57230976 None:intergenic 45.0%
TTGGAAACATTTGCCCTTCC+AGG + chr1_4:57231238-57231257 None:intergenic 45.0%
TGGAAGGGCAAATGTTTCCA+AGG - chr1_4:57231237-57231256 Msa0172270:intron 45.0%
AGCTAAAGGAGAAGCTTGCA+AGG - chr1_4:57231402-57231421 Msa0172270:intron 45.0%
TACGCTCCCGTCAAATCAAA+AGG + chr1_4:57231971-57231990 None:intergenic 45.0%
! TGATTTGACGGGAGCGTATT+TGG - chr1_4:57231973-57231992 Msa0172270:intron 45.0%
AACACGCTTATAACAGCACC+AGG + chr1_4:57232022-57232041 None:intergenic 45.0%
GAAGATCCGTGGAGTTAAGA+AGG - chr1_4:57232541-57232560 Msa0172270:intron 45.0%
!! GTATTTTTGCAGACCAAGGC+TGG - chr1_4:57232644-57232663 Msa0172270:intron 45.0%
!! GCTCTGAGAGTTGGAACGAA+GGG + chr1_4:57230817-57230836 None:intergenic 50.0%
!! AGCTCTGAGAGTTGGAACGA+AGG + chr1_4:57230818-57230837 None:intergenic 50.0%
TGGTGTGAAGGTCGAGCTAA+AGG - chr1_4:57231388-57231407 Msa0172270:intron 50.0%
ACTGACAGAACTGACACCAC+TGG + chr1_4:57232231-57232250 None:intergenic 50.0%
TAACAGGCAAAAGGAGCCCT+TGG + chr1_4:57232363-57232382 None:intergenic 50.0%
AACAGGGCAAAGAAGATCCG+TGG - chr1_4:57232530-57232549 Msa0172270:intron 50.0%
!! GCTCTGAGAGTTGGAACGAA+GGG + chr1_4:57230817-57230836 None:intergenic 50.0%
!! AGCTCTGAGAGTTGGAACGA+AGG + chr1_4:57230818-57230837 None:intergenic 50.0%
TGGTGTGAAGGTCGAGCTAA+AGG - chr1_4:57231388-57231407 Msa0172270:intron 50.0%
ACTGACAGAACTGACACCAC+TGG + chr1_4:57232231-57232250 None:intergenic 50.0%
TAACAGGCAAAAGGAGCCCT+TGG + chr1_4:57232363-57232382 None:intergenic 50.0%
AACAGGGCAAAGAAGATCCG+TGG - chr1_4:57232530-57232549 Msa0172270:intron 50.0%
CATCTCAGGTAGACAACCGG+CGG - chr1_4:57230851-57230870 Msa0172270:three_prime_UTR 55.0%
TGACCAACAGGCTTCTCTCC+AGG - chr1_4:57230936-57230955 Msa0172270:three_prime_UTR 55.0%
CTTCCTGGAGAGAAGCCTGT+TGG + chr1_4:57230942-57230961 None:intergenic 55.0%
TTGTATTGCCTGCTGGGCAC+AGG - chr1_4:57232308-57232327 Msa0172270:CDS 55.0%
AAGTAGAACCTGTGCCCAGC+AGG + chr1_4:57232319-57232338 None:intergenic 55.0%
CATCTCAGGTAGACAACCGG+CGG - chr1_4:57230851-57230870 Msa0172270:three_prime_UTR 55.0%
TGACCAACAGGCTTCTCTCC+AGG - chr1_4:57230936-57230955 Msa0172270:three_prime_UTR 55.0%
CTTCCTGGAGAGAAGCCTGT+TGG + chr1_4:57230942-57230961 None:intergenic 55.0%
TTGTATTGCCTGCTGGGCAC+AGG - chr1_4:57232308-57232327 Msa0172270:CDS 55.0%
AAGTAGAACCTGTGCCCAGC+AGG + chr1_4:57232319-57232338 None:intergenic 55.0%
! TGTTGTTGAGTTGCGGCCGC+CGG + chr1_4:57230870-57230889 None:intergenic 60.0%
TTTGGAGGCGGCAAGTCCAC+TGG - chr1_4:57231479-57231498 Msa0172270:intron 60.0%
!!! GGCGGCAAGTCCACTGGTTT+TGG - chr1_4:57231485-57231504 Msa0172270:intron 60.0%
CTTGGCAGCATCACCAGCCT+TGG + chr1_4:57232660-57232679 None:intergenic 60.0%
! CAAGGCTGGTGATGCTGCCA+AGG - chr1_4:57232658-57232677 Msa0172270:intron 60.0%
! TGTTGTTGAGTTGCGGCCGC+CGG + chr1_4:57230870-57230889 None:intergenic 60.0%
TTTGGAGGCGGCAAGTCCAC+TGG - chr1_4:57231479-57231498 Msa0172270:intron 60.0%
!!! GGCGGCAAGTCCACTGGTTT+TGG - chr1_4:57231485-57231504 Msa0172270:intron 60.0%
CTTGGCAGCATCACCAGCCT+TGG + chr1_4:57232660-57232679 None:intergenic 60.0%
! CAAGGCTGGTGATGCTGCCA+AGG - chr1_4:57232658-57232677 Msa0172270:intron 60.0%
! GCTGGTGATGCTGCCAAGGC+TGG - chr1_4:57232662-57232681 Msa0172270:intron 65.0%
! GCTGGTGATGCTGCCAAGGC+TGG - chr1_4:57232662-57232681 Msa0172270:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 57230805 57233063 57230805 ID=Msa0172270;Name=Msa0172270
chr1_4 mRNA 57230805 57233063 57230805 ID=Msa0172270-mRNA-1;Parent=Msa0172270;Name=Msa0172270-mRNA-1;_AED=0.07;_eAED=0.07;_QI=91|1|1|1|1|1|5|366|137
chr1_4 exon 57230805 57231212 57230805 ID=Msa0172270-mRNA-1:exon:17497;Parent=Msa0172270-mRNA-1
chr1_4 exon 57231306 57231392 57231306 ID=Msa0172270-mRNA-1:exon:17496;Parent=Msa0172270-mRNA-1
chr1_4 exon 57232300 57232470 57232300 ID=Msa0172270-mRNA-1:exon:17495;Parent=Msa0172270-mRNA-1
chr1_4 exon 57232610 57232651 57232610 ID=Msa0172270-mRNA-1:exon:17494;Parent=Msa0172270-mRNA-1
chr1_4 exon 57232901 57233063 57232901 ID=Msa0172270-mRNA-1:exon:17493;Parent=Msa0172270-mRNA-1
chr1_4 five_prime_UTR 57232973 57233063 57232973 ID=Msa0172270-mRNA-1:five_prime_utr;Parent=Msa0172270-mRNA-1
chr1_4 CDS 57232901 57232972 57232901 ID=Msa0172270-mRNA-1:cds;Parent=Msa0172270-mRNA-1
chr1_4 CDS 57232610 57232651 57232610 ID=Msa0172270-mRNA-1:cds;Parent=Msa0172270-mRNA-1
chr1_4 CDS 57232300 57232470 57232300 ID=Msa0172270-mRNA-1:cds;Parent=Msa0172270-mRNA-1
chr1_4 CDS 57231306 57231392 57231306 ID=Msa0172270-mRNA-1:cds;Parent=Msa0172270-mRNA-1
chr1_4 CDS 57231171 57231212 57231171 ID=Msa0172270-mRNA-1:cds;Parent=Msa0172270-mRNA-1
chr1_4 three_prime_UTR 57230805 57231170 57230805 ID=Msa0172270-mRNA-1:three_prime_utr;Parent=Msa0172270-mRNA-1
Gene Sequence

>Msa0172270

ATGGCAGACAAGGCCGTTACCATCAGAACCAGAAAGTTCATGACCAACAGGCTTCTCTCCAGGAAGCAATTCGTCATTGATGTTCTTCATCCTGGAAGGGCAAATGTTTCCAAGGTCGAGCTAAAGGAGAAGCTTGCAAGGATTTACGATGTGAAGGACCCAAACACTGTTTTTGTGTTCAAGTTCAGAACTCATTTTGGAGGCGGCAAGTCCACTGGTTTTGGTTTGATCTATGATTCTGTTGAGAATGCCAAGAAGTATGAACCAAAATACAGACTTGTTAGGAATGGACTTGATACTAAGGTTGAAAAGTCAAGGAAGCAAATGAAGGAAAGAAAGAACAGGGCAAAGAAGATCCGTGGAGTTAAGAAGACCAAGGCTGGTGATGCTGCCAAGGCTGGCAAGAAGAAATAA
Protein sequence

>Msa0172270

MADKAVTIRTRKFMTNRLLSRKQFVIDVLHPGRANVSKVELKEKLARIYDVKDPNTVFVFKFRTHFGGGKSTGFGLIYDSVENAKKYEPKYRLVRNGLDTKVEKSRKQMKERKNRAKKIRGVKKTKAGDAAKAGKKK*