AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0683660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683660 MtrunA17_Chr4g0030641 95.041 121 6 0 2 122 3 123 1.23e-76 222
Msa0683660 MtrunA17_Chr1g0182051 92.623 122 9 0 1 122 1 122 1.67e-75 219
Msa0683660 MtrunA17_Chr4g0036351 91.597 119 10 0 4 122 3 121 9.30e-72 209
Msa0683660 MtrunA17_Chr1g0212921 90.000 120 12 0 3 122 2 121 1.54e-71 209
Msa0683660 MtrunA17_Chr7g0249361 85.000 120 18 0 3 122 49 168 3.12e-65 195
Msa0683660 MtrunA17_Chr1g0206641 87.179 39 5 0 10 48 2 40 2.99e-15 64.7
Msa0683660 MtrunA17_Chr5g0419591 82.353 34 6 0 66 99 2 35 1.12e-12 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0683660 AT3G09500.1 86.555 119 16 0 3 121 4 122 4.80e-68 201
Msa0683660 AT5G02610.1 87.395 119 15 0 3 121 4 122 5.02e-68 201
Msa0683660 AT2G39390.1 86.555 119 16 0 3 121 4 122 6.11e-68 201
Msa0683660 AT3G55170.5 84.034 119 19 0 3 121 4 122 2.26e-65 194
Msa0683660 AT3G55170.2 84.034 119 19 0 3 121 4 122 2.26e-65 194
Msa0683660 AT3G55170.1 84.034 119 19 0 3 121 4 122 2.26e-65 194
Msa0683660 AT5G02610.2 73.239 142 15 1 3 121 4 145 2.74e-62 187
Msa0683660 AT3G55170.4 83.505 97 16 0 3 99 4 100 1.87e-50 155
Msa0683660 AT3G55170.3 83.505 97 16 0 3 99 4 100 1.87e-50 155

Find 45 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTAATAGCTCTCGTCTTCT+TGG 0.242711 4_4:+46488588 None:intergenic
AGCATTGAGGGAAGTTTATA+AGG 0.261646 4_4:-46488641 Msa0683660:CDS
TACATCTTGGAGAGCTTATT+GGG 0.264921 4_4:+46488914 None:intergenic
GACAATGTTCTAGAAATATT+TGG 0.270088 4_4:-46487213 Msa0683660:three_prime_UTR
GGTCCAACATTACGCCTTAA+TGG 0.287059 4_4:+46487268 None:intergenic
ATACATCTTGGAGAGCTTAT+TGG 0.293485 4_4:+46488913 None:intergenic
ATATGCGACTATTTGTATAT+TGG 0.314859 4_4:+46487043 None:intergenic
AGCATTAAATAATCTGAAAC+TGG 0.347111 4_4:+46487096 None:intergenic
TTTAACTTATCTTCATGAAA+TGG 0.348353 4_4:-46487160 Msa0683660:three_prime_UTR
CATGAATTTCGATTTGTTGT+TGG 0.373914 4_4:-46487235 Msa0683660:three_prime_UTR
CTAATAGCTCTCGTCTTCTT+GGG 0.390815 4_4:+46488589 None:intergenic
TAGCATGAATGAGTGACTTA+TGG 0.401589 4_4:-46487000 Msa0683660:three_prime_UTR
AGTGAGAAAGTATGCCATTA+AGG 0.406198 4_4:-46487282 Msa0683660:CDS
GCATTGAGGGAAGTTTATAA+GGG 0.408192 4_4:-46488640 Msa0683660:CDS
GGGGAAGAAGTACTTGCCTT+TGG 0.408254 4_4:-46488620 Msa0683660:CDS
TTTGAACGCAATAGCTATCA+AGG 0.421455 4_4:-46489041 Msa0683660:intron
TCGAAATTCATGCAAAAGAA+AGG 0.425679 4_4:+46487246 None:intergenic
CGATTCAAGATCTGCGAAAT+GGG 0.431683 4_4:-46489159 Msa0683660:intron
GCGATTCAAGATCTGCGAAA+TGG 0.438230 4_4:-46489160 Msa0683660:exon
TTGGATGCAGCAAGGTTGTT+CGG 0.445039 4_4:-46488711 Msa0683660:intron
GAAGATAAGTTAAACTCAAA+AGG 0.456428 4_4:+46487169 None:intergenic
GAATGAGTGACTTATGGTTT+GGG 0.478655 4_4:-46486994 Msa0683660:three_prime_UTR
GAAGTCACGTTGAAAACATA+GGG 0.482415 4_4:+46486941 None:intergenic
TGAATGAGTGACTTATGGTT+TGG 0.486189 4_4:-46486995 Msa0683660:three_prime_UTR
ATCGCCGCCAGAAAACTTGT+TGG 0.498743 4_4:+46489178 None:intergenic
GAAACAAAAGACAGCATTGA+GGG 0.506620 4_4:-46488653 Msa0683660:CDS
CTTCGCGTCGCGAAAGTCAC+CGG 0.510669 4_4:-46488944 Msa0683660:CDS
CGAAATTCATGCAAAAGAAA+GGG 0.512267 4_4:+46487247 None:intergenic
AAGACGCCTCACTAAGCATC+AGG 0.512300 4_4:-46488566 Msa0683660:intron
TTAATGGCATACTTTCTCAC+TGG 0.517469 4_4:+46487284 None:intergenic
AGAAGTCACGTTGAAAACAT+AGG 0.522548 4_4:+46486940 None:intergenic
TTCTTGGGACGGAGATCCAA+AGG 0.574336 4_4:+46488604 None:intergenic
TAATGGCATACTTTCTCACT+GGG 0.582481 4_4:+46487285 None:intergenic
GACAGAGCGTGAGAAGAAGA+AGG 0.584517 4_4:-46487318 Msa0683660:CDS
ATGCCATTAAGGCGTAATGT+TGG 0.585342 4_4:-46487271 Msa0683660:exon
CGGTGACTTTCGCGACGCGA+AGG 0.585891 4_4:+46488945 None:intergenic
TAGCTCTCGTCTTCTTGGGA+CGG 0.610807 4_4:+46488593 None:intergenic
CTATCAAGGTTTATGAGCTG+AGG 0.615557 4_4:-46489027 Msa0683660:CDS
ACATCTTGGAGAGCTTATTG+GGG 0.615612 4_4:+46488915 None:intergenic
CATTGAGGGAAGTTTATAAG+GGG 0.623355 4_4:-46488639 Msa0683660:CDS
CGCGTCGCGAAAGTCACCGG+CGG 0.629305 4_4:-46488941 Msa0683660:CDS
TATGTACCTGATGCTTAGTG+AGG 0.656359 4_4:+46488560 None:intergenic
AGAAACAAAAGACAGCATTG+AGG 0.664403 4_4:-46488654 Msa0683660:CDS
CATCTTGGAGAGCTTATTGG+GGG 0.689364 4_4:+46488916 None:intergenic
AAGTCACGTTGAAAACATAG+GGG 0.718692 4_4:+46486942 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCATAATTATTTATG+TGG - chr4_4:46488334-46488353 Msa0683660:intron 10.0%
!!! TTTTTTCATAATTATTTATG+TGG - chr4_4:46488334-46488353 Msa0683660:intron 10.0%
!! AAATTTAGAGAATAGAATAA+AGG + chr4_4:46487017-46487036 None:intergenic 15.0%
!! TGAAATAATTTGATGAAATT+CGG - chr4_4:46487651-46487670 Msa0683660:intron 15.0%
!! ATTATGAAAAAATGAGTAAA+TGG + chr4_4:46488326-46488345 None:intergenic 15.0%
!! TTTGTTACAATTATAATTGT+GGG - chr4_4:46488387-46488406 Msa0683660:intron 15.0%
!! AAATTTAGAGAATAGAATAA+AGG + chr4_4:46487017-46487036 None:intergenic 15.0%
!! TGAAATAATTTGATGAAATT+CGG - chr4_4:46487651-46487670 Msa0683660:intron 15.0%
!! ATTATGAAAAAATGAGTAAA+TGG + chr4_4:46488326-46488345 None:intergenic 15.0%
!! TTTGTTACAATTATAATTGT+GGG - chr4_4:46488387-46488406 Msa0683660:intron 15.0%
!!! TCATCTATAGTAATTTTGAT+TGG + chr4_4:46487351-46487370 None:intergenic 20.0%
!!! TGATTGTTTTTGTTATTGTT+TGG - chr4_4:46487404-46487423 Msa0683660:intron 20.0%
!! CTTTGTTACAATTATAATTG+TGG - chr4_4:46488386-46488405 Msa0683660:intron 20.0%
!! TTGTTACAATTATAATTGTG+GGG - chr4_4:46488388-46488407 Msa0683660:intron 20.0%
!! TATAAGCTTTCTTCATAAAA+TGG - chr4_4:46488752-46488771 Msa0683660:intron 20.0%
!!! TTTAACTTATCTTCATGAAA+TGG - chr4_4:46488974-46488993 Msa0683660:CDS 20.0%
!!! TCATCTATAGTAATTTTGAT+TGG + chr4_4:46487351-46487370 None:intergenic 20.0%
!!! TGATTGTTTTTGTTATTGTT+TGG - chr4_4:46487404-46487423 Msa0683660:intron 20.0%
!! CTTTGTTACAATTATAATTG+TGG - chr4_4:46488386-46488405 Msa0683660:intron 20.0%
!! TTGTTACAATTATAATTGTG+GGG - chr4_4:46488388-46488407 Msa0683660:intron 20.0%
!! TATAAGCTTTCTTCATAAAA+TGG - chr4_4:46488752-46488771 Msa0683660:intron 20.0%
!!! TTTAACTTATCTTCATGAAA+TGG - chr4_4:46488974-46488993 Msa0683660:CDS 20.0%
! TAGAGAATAGAATAAAGGAT+TGG + chr4_4:46487012-46487031 None:intergenic 25.0%
! ATGAGTACAAAAAATGATGT+TGG + chr4_4:46487044-46487063 None:intergenic 25.0%
! ATGAAATTCGGACTTATATT+TGG - chr4_4:46487663-46487682 Msa0683660:intron 25.0%
!! TGAATAGTTTGTAAAGTTTG+TGG - chr4_4:46487735-46487754 Msa0683660:intron 25.0%
!! TCAATGATTTTCATGATGTA+TGG - chr4_4:46487790-46487809 Msa0683660:intron 25.0%
!! AAGCTATCTTTGTAGATTTT+TGG - chr4_4:46487861-46487880 Msa0683660:intron 25.0%
!! AGCTATCTTTGTAGATTTTT+GGG - chr4_4:46487862-46487881 Msa0683660:intron 25.0%
! TGAAAAAATGAGTAAATGGT+TGG + chr4_4:46488322-46488341 None:intergenic 25.0%
!! ATTTTATCAATGTGCCTTAT+TGG - chr4_4:46488457-46488476 Msa0683660:intron 25.0%
!!! GACAATGTTCTAGAAATATT+TGG - chr4_4:46488921-46488940 Msa0683660:CDS 25.0%
! GAAGATAAGTTAAACTCAAA+AGG + chr4_4:46488968-46488987 None:intergenic 25.0%
!! AGCATTAAATAATCTGAAAC+TGG + chr4_4:46489041-46489060 None:intergenic 25.0%
! ATATGCGACTATTTGTATAT+TGG + chr4_4:46489094-46489113 None:intergenic 25.0%
! TAGAGAATAGAATAAAGGAT+TGG + chr4_4:46487012-46487031 None:intergenic 25.0%
! ATGAGTACAAAAAATGATGT+TGG + chr4_4:46487044-46487063 None:intergenic 25.0%
! ATGAAATTCGGACTTATATT+TGG - chr4_4:46487663-46487682 Msa0683660:intron 25.0%
!! TGAATAGTTTGTAAAGTTTG+TGG - chr4_4:46487735-46487754 Msa0683660:intron 25.0%
!! TCAATGATTTTCATGATGTA+TGG - chr4_4:46487790-46487809 Msa0683660:intron 25.0%
!! AAGCTATCTTTGTAGATTTT+TGG - chr4_4:46487861-46487880 Msa0683660:intron 25.0%
!! AGCTATCTTTGTAGATTTTT+GGG - chr4_4:46487862-46487881 Msa0683660:intron 25.0%
! TGAAAAAATGAGTAAATGGT+TGG + chr4_4:46488322-46488341 None:intergenic 25.0%
!! ATTTTATCAATGTGCCTTAT+TGG - chr4_4:46488457-46488476 Msa0683660:intron 25.0%
!!! GACAATGTTCTAGAAATATT+TGG - chr4_4:46488921-46488940 Msa0683660:CDS 25.0%
! GAAGATAAGTTAAACTCAAA+AGG + chr4_4:46488968-46488987 None:intergenic 25.0%
!! AGCATTAAATAATCTGAAAC+TGG + chr4_4:46489041-46489060 None:intergenic 25.0%
! ATATGCGACTATTTGTATAT+TGG + chr4_4:46489094-46489113 None:intergenic 25.0%
TTGCGTTCAAAAAAGAAGAA+AGG + chr4_4:46487084-46487103 None:intergenic 30.0%
AATTAAGTGCAATAGAAGAG+CGG + chr4_4:46487254-46487273 None:intergenic 30.0%
GACTTATATTTGGTATGCTT+TGG - chr4_4:46487673-46487692 Msa0683660:intron 30.0%
TTCTGCTCAATTATTGTTCT+AGG - chr4_4:46487825-46487844 Msa0683660:intron 30.0%
TCTGCTCAATTATTGTTCTA+GGG - chr4_4:46487826-46487845 Msa0683660:intron 30.0%
ATTGTGGAATATAAGAACAG+TGG - chr4_4:46488060-46488079 Msa0683660:intron 30.0%
ATTTATGTGGACAATACTTG+TGG - chr4_4:46488347-46488366 Msa0683660:intron 30.0%
GTGATATATTTGCCTCTTAT+CGG - chr4_4:46488487-46488506 Msa0683660:intron 30.0%
TGATATATTTGCCTCTTATC+GGG - chr4_4:46488488-46488507 Msa0683660:intron 30.0%
AAAACATGTATGTATGTGCT+CGG + chr4_4:46488604-46488623 None:intergenic 30.0%
!!! ACATACATGTTTTGACTTCT+AGG - chr4_4:46488611-46488630 Msa0683660:CDS 30.0%
!!! CATACATGTTTTGACTTCTA+GGG - chr4_4:46488612-46488631 Msa0683660:CDS 30.0%
AAATGGCTGAATGAATTTAC+TGG - chr4_4:46488769-46488788 Msa0683660:intron 30.0%
AATGGCTGAATGAATTTACT+GGG - chr4_4:46488770-46488789 Msa0683660:intron 30.0%
CGAAATTCATGCAAAAGAAA+GGG + chr4_4:46488890-46488909 None:intergenic 30.0%
TCGAAATTCATGCAAAAGAA+AGG + chr4_4:46488891-46488910 None:intergenic 30.0%
!! CATGAATTTCGATTTGTTGT+TGG - chr4_4:46488899-46488918 Msa0683660:intron 30.0%
TTGCGTTCAAAAAAGAAGAA+AGG + chr4_4:46487084-46487103 None:intergenic 30.0%
AATTAAGTGCAATAGAAGAG+CGG + chr4_4:46487254-46487273 None:intergenic 30.0%
GACTTATATTTGGTATGCTT+TGG - chr4_4:46487673-46487692 Msa0683660:intron 30.0%
TTCTGCTCAATTATTGTTCT+AGG - chr4_4:46487825-46487844 Msa0683660:intron 30.0%
TCTGCTCAATTATTGTTCTA+GGG - chr4_4:46487826-46487845 Msa0683660:intron 30.0%
ATTGTGGAATATAAGAACAG+TGG - chr4_4:46488060-46488079 Msa0683660:intron 30.0%
ATTTATGTGGACAATACTTG+TGG - chr4_4:46488347-46488366 Msa0683660:intron 30.0%
GTGATATATTTGCCTCTTAT+CGG - chr4_4:46488487-46488506 Msa0683660:intron 30.0%
TGATATATTTGCCTCTTATC+GGG - chr4_4:46488488-46488507 Msa0683660:intron 30.0%
AAAACATGTATGTATGTGCT+CGG + chr4_4:46488604-46488623 None:intergenic 30.0%
!!! ACATACATGTTTTGACTTCT+AGG - chr4_4:46488611-46488630 Msa0683660:CDS 30.0%
!!! CATACATGTTTTGACTTCTA+GGG - chr4_4:46488612-46488631 Msa0683660:CDS 30.0%
AAATGGCTGAATGAATTTAC+TGG - chr4_4:46488769-46488788 Msa0683660:intron 30.0%
AATGGCTGAATGAATTTACT+GGG - chr4_4:46488770-46488789 Msa0683660:intron 30.0%
CGAAATTCATGCAAAAGAAA+GGG + chr4_4:46488890-46488909 None:intergenic 30.0%
TCGAAATTCATGCAAAAGAA+AGG + chr4_4:46488891-46488910 None:intergenic 30.0%
!! CATGAATTTCGATTTGTTGT+TGG - chr4_4:46488899-46488918 Msa0683660:intron 30.0%
! CTTTCTACCAACAAGTTTTC+TGG - chr4_4:46486949-46486968 Msa0683660:three_prime_UTR 35.0%
TTTGAACGCAATAGCTATCA+AGG - chr4_4:46487093-46487112 Msa0683660:three_prime_UTR 35.0%
TACATCTTGGAGAGCTTATT+GGG + chr4_4:46487223-46487242 None:intergenic 35.0%
ATACATCTTGGAGAGCTTAT+TGG + chr4_4:46487224-46487243 None:intergenic 35.0%
AGAAACAAAAGACAGCATTG+AGG - chr4_4:46487480-46487499 Msa0683660:intron 35.0%
! GAAACAAAAGACAGCATTGA+GGG - chr4_4:46487481-46487500 Msa0683660:intron 35.0%
! AGCATTGAGGGAAGTTTATA+AGG - chr4_4:46487493-46487512 Msa0683660:intron 35.0%
GCATTGAGGGAAGTTTATAA+GGG - chr4_4:46487494-46487513 Msa0683660:intron 35.0%
CATTGAGGGAAGTTTATAAG+GGG - chr4_4:46487495-46487514 Msa0683660:intron 35.0%
GCAAGTGTTTCTTTCTATGA+AGG - chr4_4:46487612-46487631 Msa0683660:intron 35.0%
!! CTTAATCTTTGACTTGCTGA+AGG + chr4_4:46488249-46488268 None:intergenic 35.0%
AGATTAAGCTTGAACTTGCA+TGG - chr4_4:46488261-46488280 Msa0683660:intron 35.0%
ATTAATGGTACCACATGTTG+TGG + chr4_4:46488293-46488312 None:intergenic 35.0%
ACATGTGGTACCATTAATTG+AGG - chr4_4:46488295-46488314 Msa0683660:intron 35.0%
! AATGGTTGGTCCTCAATTAA+TGG + chr4_4:46488308-46488327 None:intergenic 35.0%
ACAATTATAATTGTGGGGAC+TGG - chr4_4:46488393-46488412 Msa0683660:intron 35.0%
CAATTATAATTGTGGGGACT+GGG - chr4_4:46488394-46488413 Msa0683660:intron 35.0%
AATTATAATTGTGGGGACTG+GGG - chr4_4:46488395-46488414 Msa0683660:intron 35.0%
ATATCACCTATGCTCCAATA+AGG + chr4_4:46488474-46488493 None:intergenic 35.0%
AAGTTAAAACTCCCGATAAG+AGG + chr4_4:46488502-46488521 None:intergenic 35.0%
AAACATGTATGTATGTGCTC+GGG + chr4_4:46488603-46488622 None:intergenic 35.0%
ACAACTTCACCAACAAAGAT+TGG + chr4_4:46488638-46488657 None:intergenic 35.0%
CAACCATTCATGAAAAGTCA+AGG + chr4_4:46488720-46488739 None:intergenic 35.0%
TAATGGCATACTTTCTCACT+GGG + chr4_4:46488852-46488871 None:intergenic 35.0%
TTAATGGCATACTTTCTCAC+TGG + chr4_4:46488853-46488872 None:intergenic 35.0%
! AGTGAGAAAGTATGCCATTA+AGG - chr4_4:46488852-46488871 Msa0683660:intron 35.0%
TAGCATGAATGAGTGACTTA+TGG - chr4_4:46489134-46489153 Msa0683660:intron 35.0%
TGAATGAGTGACTTATGGTT+TGG - chr4_4:46489139-46489158 Msa0683660:intron 35.0%
GAATGAGTGACTTATGGTTT+GGG - chr4_4:46489140-46489159 Msa0683660:intron 35.0%
AAGTCACGTTGAAAACATAG+GGG + chr4_4:46489195-46489214 None:intergenic 35.0%
GAAGTCACGTTGAAAACATA+GGG + chr4_4:46489196-46489215 None:intergenic 35.0%
AGAAGTCACGTTGAAAACAT+AGG + chr4_4:46489197-46489216 None:intergenic 35.0%
!! TTGGTAGAAAGTGCAAGTTT+TGG + chr4_4:46486940-46486959 None:intergenic 35.0%
! CTTTCTACCAACAAGTTTTC+TGG - chr4_4:46486949-46486968 Msa0683660:three_prime_UTR 35.0%
TTTGAACGCAATAGCTATCA+AGG - chr4_4:46487093-46487112 Msa0683660:three_prime_UTR 35.0%
TACATCTTGGAGAGCTTATT+GGG + chr4_4:46487223-46487242 None:intergenic 35.0%
ATACATCTTGGAGAGCTTAT+TGG + chr4_4:46487224-46487243 None:intergenic 35.0%
AGAAACAAAAGACAGCATTG+AGG - chr4_4:46487480-46487499 Msa0683660:intron 35.0%
! GAAACAAAAGACAGCATTGA+GGG - chr4_4:46487481-46487500 Msa0683660:intron 35.0%
! AGCATTGAGGGAAGTTTATA+AGG - chr4_4:46487493-46487512 Msa0683660:intron 35.0%
GCATTGAGGGAAGTTTATAA+GGG - chr4_4:46487494-46487513 Msa0683660:intron 35.0%
CATTGAGGGAAGTTTATAAG+GGG - chr4_4:46487495-46487514 Msa0683660:intron 35.0%
GCAAGTGTTTCTTTCTATGA+AGG - chr4_4:46487612-46487631 Msa0683660:intron 35.0%
!! CTTAATCTTTGACTTGCTGA+AGG + chr4_4:46488249-46488268 None:intergenic 35.0%
AGATTAAGCTTGAACTTGCA+TGG - chr4_4:46488261-46488280 Msa0683660:intron 35.0%
ATTAATGGTACCACATGTTG+TGG + chr4_4:46488293-46488312 None:intergenic 35.0%
ACATGTGGTACCATTAATTG+AGG - chr4_4:46488295-46488314 Msa0683660:intron 35.0%
! AATGGTTGGTCCTCAATTAA+TGG + chr4_4:46488308-46488327 None:intergenic 35.0%
ACAATTATAATTGTGGGGAC+TGG - chr4_4:46488393-46488412 Msa0683660:intron 35.0%
CAATTATAATTGTGGGGACT+GGG - chr4_4:46488394-46488413 Msa0683660:intron 35.0%
AATTATAATTGTGGGGACTG+GGG - chr4_4:46488395-46488414 Msa0683660:intron 35.0%
ATATCACCTATGCTCCAATA+AGG + chr4_4:46488474-46488493 None:intergenic 35.0%
AAGTTAAAACTCCCGATAAG+AGG + chr4_4:46488502-46488521 None:intergenic 35.0%
AAACATGTATGTATGTGCTC+GGG + chr4_4:46488603-46488622 None:intergenic 35.0%
ACAACTTCACCAACAAAGAT+TGG + chr4_4:46488638-46488657 None:intergenic 35.0%
CAACCATTCATGAAAAGTCA+AGG + chr4_4:46488720-46488739 None:intergenic 35.0%
TAATGGCATACTTTCTCACT+GGG + chr4_4:46488852-46488871 None:intergenic 35.0%
TTAATGGCATACTTTCTCAC+TGG + chr4_4:46488853-46488872 None:intergenic 35.0%
! AGTGAGAAAGTATGCCATTA+AGG - chr4_4:46488852-46488871 Msa0683660:intron 35.0%
TAGCATGAATGAGTGACTTA+TGG - chr4_4:46489134-46489153 Msa0683660:intron 35.0%
TGAATGAGTGACTTATGGTT+TGG - chr4_4:46489139-46489158 Msa0683660:intron 35.0%
GAATGAGTGACTTATGGTTT+GGG - chr4_4:46489140-46489159 Msa0683660:intron 35.0%
AAGTCACGTTGAAAACATAG+GGG + chr4_4:46489195-46489214 None:intergenic 35.0%
GAAGTCACGTTGAAAACATA+GGG + chr4_4:46489196-46489215 None:intergenic 35.0%
AGAAGTCACGTTGAAAACAT+AGG + chr4_4:46489197-46489216 None:intergenic 35.0%
! TCTACCAACAAGTTTTCTGG+CGG - chr4_4:46486952-46486971 Msa0683660:three_prime_UTR 40.0%
CGATTCAAGATCTGCGAAAT+GGG - chr4_4:46486975-46486994 Msa0683660:three_prime_UTR 40.0%
CTATCAAGGTTTATGAGCTG+AGG - chr4_4:46487107-46487126 Msa0683660:three_prime_UTR 40.0%
ACATCTTGGAGAGCTTATTG+GGG + chr4_4:46487222-46487241 None:intergenic 40.0%
AGCGGAAAATGCATACATCT+TGG + chr4_4:46487236-46487255 None:intergenic 40.0%
GTTATTGTTTGGATGCAGCA+AGG - chr4_4:46487415-46487434 Msa0683660:intron 40.0%
CTAATAGCTCTCGTCTTCTT+GGG + chr4_4:46487548-46487567 None:intergenic 40.0%
TCTAATAGCTCTCGTCTTCT+TGG + chr4_4:46487549-46487568 None:intergenic 40.0%
TATGTACCTGATGCTTAGTG+AGG + chr4_4:46487577-46487596 None:intergenic 40.0%
GACAACACTTTGTACTACAG+CGG + chr4_4:46487960-46487979 None:intergenic 40.0%
TCGCAGATGACAGAAAATAG+CGG + chr4_4:46488032-46488051 None:intergenic 40.0%
! CTATTTTCTGTCATCTGCGA+CGG - chr4_4:46488032-46488051 Msa0683660:intron 40.0%
TGGAAGTAATAGAGGACTTC+GGG + chr4_4:46488128-46488147 None:intergenic 40.0%
GTGGAAGTAATAGAGGACTT+CGG + chr4_4:46488129-46488148 None:intergenic 40.0%
! GAAGTGTTTGTCTCAAGGAA+GGG + chr4_4:46488179-46488198 None:intergenic 40.0%
!! TTAGGGAAGTGTTTGTCTCA+AGG + chr4_4:46488184-46488203 None:intergenic 40.0%
!! TTTGACTTGCTGAAGGAATG+TGG + chr4_4:46488242-46488261 None:intergenic 40.0%
AGCTTGAACTTGCATGGTTT+AGG - chr4_4:46488267-46488286 Msa0683660:intron 40.0%
ATTATAATTGTGGGGACTGG+GGG - chr4_4:46488396-46488415 Msa0683660:intron 40.0%
! GTTGTAGAGTTTTGCTGCAA+TGG - chr4_4:46488430-46488449 Msa0683660:intron 40.0%
! AATGTGCCTTATTGGAGCAT+AGG - chr4_4:46488465-46488484 Msa0683660:intron 40.0%
! GTGCCTTGACTTTTCATGAA+TGG - chr4_4:46488714-46488733 Msa0683660:intron 40.0%
ATGCCATTAAGGCGTAATGT+TGG - chr4_4:46488863-46488882 Msa0683660:intron 40.0%
! TCTACCAACAAGTTTTCTGG+CGG - chr4_4:46486952-46486971 Msa0683660:three_prime_UTR 40.0%
CGATTCAAGATCTGCGAAAT+GGG - chr4_4:46486975-46486994 Msa0683660:three_prime_UTR 40.0%
CTATCAAGGTTTATGAGCTG+AGG - chr4_4:46487107-46487126 Msa0683660:three_prime_UTR 40.0%
ACATCTTGGAGAGCTTATTG+GGG + chr4_4:46487222-46487241 None:intergenic 40.0%
AGCGGAAAATGCATACATCT+TGG + chr4_4:46487236-46487255 None:intergenic 40.0%
GTTATTGTTTGGATGCAGCA+AGG - chr4_4:46487415-46487434 Msa0683660:intron 40.0%
CTAATAGCTCTCGTCTTCTT+GGG + chr4_4:46487548-46487567 None:intergenic 40.0%
TCTAATAGCTCTCGTCTTCT+TGG + chr4_4:46487549-46487568 None:intergenic 40.0%
TATGTACCTGATGCTTAGTG+AGG + chr4_4:46487577-46487596 None:intergenic 40.0%
GACAACACTTTGTACTACAG+CGG + chr4_4:46487960-46487979 None:intergenic 40.0%
TCGCAGATGACAGAAAATAG+CGG + chr4_4:46488032-46488051 None:intergenic 40.0%
! CTATTTTCTGTCATCTGCGA+CGG - chr4_4:46488032-46488051 Msa0683660:intron 40.0%
TGGAAGTAATAGAGGACTTC+GGG + chr4_4:46488128-46488147 None:intergenic 40.0%
GTGGAAGTAATAGAGGACTT+CGG + chr4_4:46488129-46488148 None:intergenic 40.0%
! GAAGTGTTTGTCTCAAGGAA+GGG + chr4_4:46488179-46488198 None:intergenic 40.0%
!! TTAGGGAAGTGTTTGTCTCA+AGG + chr4_4:46488184-46488203 None:intergenic 40.0%
!! TTTGACTTGCTGAAGGAATG+TGG + chr4_4:46488242-46488261 None:intergenic 40.0%
AGCTTGAACTTGCATGGTTT+AGG - chr4_4:46488267-46488286 Msa0683660:intron 40.0%
ATTATAATTGTGGGGACTGG+GGG - chr4_4:46488396-46488415 Msa0683660:intron 40.0%
! GTTGTAGAGTTTTGCTGCAA+TGG - chr4_4:46488430-46488449 Msa0683660:intron 40.0%
! AATGTGCCTTATTGGAGCAT+AGG - chr4_4:46488465-46488484 Msa0683660:intron 40.0%
! GTGCCTTGACTTTTCATGAA+TGG - chr4_4:46488714-46488733 Msa0683660:intron 40.0%
ATGCCATTAAGGCGTAATGT+TGG - chr4_4:46488863-46488882 Msa0683660:intron 40.0%
GCGATTCAAGATCTGCGAAA+TGG - chr4_4:46486974-46486993 Msa0683660:three_prime_UTR 45.0%
CATCTTGGAGAGCTTATTGG+GGG + chr4_4:46487221-46487240 None:intergenic 45.0%
TTGGATGCAGCAAGGTTGTT+CGG - chr4_4:46487423-46487442 Msa0683660:intron 45.0%
CGGATAGCAGAACAATACAC+AGG + chr4_4:46487940-46487959 None:intergenic 45.0%
GCTATAGCGTGGTAGTATAG+CGG - chr4_4:46487995-46488014 Msa0683660:intron 45.0%
ATCTGCGACGGACAATATTG+TGG - chr4_4:46488044-46488063 Msa0683660:intron 45.0%
AAGTAATAGAGGACTTCGGG+AGG + chr4_4:46488125-46488144 None:intergenic 45.0%
GGAGCAAGTGGAAGTAATAG+AGG + chr4_4:46488136-46488155 None:intergenic 45.0%
TGTTTGTCTCAAGGAAGGGA+GGG + chr4_4:46488175-46488194 None:intergenic 45.0%
!! GGAAGTGTTTGTCTCAAGGA+AGG + chr4_4:46488180-46488199 None:intergenic 45.0%
GACAAACACTTCCCTAAGCT+TGG - chr4_4:46488187-46488206 Msa0683660:intron 45.0%
ATGGTTTAGGCCACAACATG+TGG - chr4_4:46488280-46488299 Msa0683660:intron 45.0%
!! CTTCTAGGGCCAATCTTTGT+TGG - chr4_4:46488626-46488645 Msa0683660:CDS 45.0%
GGTCCAACATTACGCCTTAA+TGG + chr4_4:46488869-46488888 None:intergenic 45.0%
GCGATTCAAGATCTGCGAAA+TGG - chr4_4:46486974-46486993 Msa0683660:three_prime_UTR 45.0%
CATCTTGGAGAGCTTATTGG+GGG + chr4_4:46487221-46487240 None:intergenic 45.0%
TTGGATGCAGCAAGGTTGTT+CGG - chr4_4:46487423-46487442 Msa0683660:intron 45.0%
CGGATAGCAGAACAATACAC+AGG + chr4_4:46487940-46487959 None:intergenic 45.0%
GCTATAGCGTGGTAGTATAG+CGG - chr4_4:46487995-46488014 Msa0683660:intron 45.0%
ATCTGCGACGGACAATATTG+TGG - chr4_4:46488044-46488063 Msa0683660:intron 45.0%
AAGTAATAGAGGACTTCGGG+AGG + chr4_4:46488125-46488144 None:intergenic 45.0%
GGAGCAAGTGGAAGTAATAG+AGG + chr4_4:46488136-46488155 None:intergenic 45.0%
TGTTTGTCTCAAGGAAGGGA+GGG + chr4_4:46488175-46488194 None:intergenic 45.0%
!! GGAAGTGTTTGTCTCAAGGA+AGG + chr4_4:46488180-46488199 None:intergenic 45.0%
GACAAACACTTCCCTAAGCT+TGG - chr4_4:46488187-46488206 Msa0683660:intron 45.0%
ATGGTTTAGGCCACAACATG+TGG - chr4_4:46488280-46488299 Msa0683660:intron 45.0%
!! CTTCTAGGGCCAATCTTTGT+TGG - chr4_4:46488626-46488645 Msa0683660:CDS 45.0%
GGTCCAACATTACGCCTTAA+TGG + chr4_4:46488869-46488888 None:intergenic 45.0%
ATCGCCGCCAGAAAACTTGT+TGG + chr4_4:46486959-46486978 None:intergenic 50.0%
!! GGGGAAGAAGTACTTGCCTT+TGG - chr4_4:46487514-46487533 Msa0683660:intron 50.0%
TTCTTGGGACGGAGATCCAA+AGG + chr4_4:46487533-46487552 None:intergenic 50.0%
TAGCTCTCGTCTTCTTGGGA+CGG + chr4_4:46487544-46487563 None:intergenic 50.0%
AAGACGCCTCACTAAGCATC+AGG - chr4_4:46487568-46487587 Msa0683660:intron 50.0%
AGTGTTGTCAGCCGCTATTG+CGG - chr4_4:46487971-46487990 Msa0683660:intron 50.0%
ACGCTATAGCACCGCAATAG+CGG + chr4_4:46487985-46488004 None:intergenic 50.0%
TAGAGGACTTCGGGAGGAAA+GGG + chr4_4:46488119-46488138 None:intergenic 50.0%
ATAGAGGACTTCGGGAGGAA+AGG + chr4_4:46488120-46488139 None:intergenic 50.0%
GTGTTTGTCTCAAGGAAGGG+AGG + chr4_4:46488176-46488195 None:intergenic 50.0%
ACTTCCCTAAGCTTGGAACG+TGG - chr4_4:46488194-46488213 Msa0683660:intron 50.0%
TCTGCCACGTTCCAAGCTTA+GGG + chr4_4:46488201-46488220 None:intergenic 50.0%
ATCTGCCACGTTCCAAGCTT+AGG + chr4_4:46488202-46488221 None:intergenic 50.0%
GACAGAGCGTGAGAAGAAGA+AGG - chr4_4:46488816-46488835 Msa0683660:intron 50.0%
ATCGCCGCCAGAAAACTTGT+TGG + chr4_4:46486959-46486978 None:intergenic 50.0%
!! GGGGAAGAAGTACTTGCCTT+TGG - chr4_4:46487514-46487533 Msa0683660:intron 50.0%
TTCTTGGGACGGAGATCCAA+AGG + chr4_4:46487533-46487552 None:intergenic 50.0%
TAGCTCTCGTCTTCTTGGGA+CGG + chr4_4:46487544-46487563 None:intergenic 50.0%
AAGACGCCTCACTAAGCATC+AGG - chr4_4:46487568-46487587 Msa0683660:intron 50.0%
AGTGTTGTCAGCCGCTATTG+CGG - chr4_4:46487971-46487990 Msa0683660:intron 50.0%
ACGCTATAGCACCGCAATAG+CGG + chr4_4:46487985-46488004 None:intergenic 50.0%
TAGAGGACTTCGGGAGGAAA+GGG + chr4_4:46488119-46488138 None:intergenic 50.0%
ATAGAGGACTTCGGGAGGAA+AGG + chr4_4:46488120-46488139 None:intergenic 50.0%
GTGTTTGTCTCAAGGAAGGG+AGG + chr4_4:46488176-46488195 None:intergenic 50.0%
ACTTCCCTAAGCTTGGAACG+TGG - chr4_4:46488194-46488213 Msa0683660:intron 50.0%
TCTGCCACGTTCCAAGCTTA+GGG + chr4_4:46488201-46488220 None:intergenic 50.0%
ATCTGCCACGTTCCAAGCTT+AGG + chr4_4:46488202-46488221 None:intergenic 50.0%
GACAGAGCGTGAGAAGAAGA+AGG - chr4_4:46488816-46488835 Msa0683660:intron 50.0%
!! GCTATTGCGGTGCTATAGCG+TGG - chr4_4:46487984-46488003 Msa0683660:intron 55.0%
TTCGGGAGGAAAGGGCTTTG+CGG + chr4_4:46488111-46488130 None:intergenic 55.0%
GGAAAGGAGGAAGGAGCAAG+TGG + chr4_4:46488148-46488167 None:intergenic 55.0%
TTGTGGGGACTGGGGGAAAT+TGG - chr4_4:46488403-46488422 Msa0683660:intron 55.0%
!! GCTATTGCGGTGCTATAGCG+TGG - chr4_4:46487984-46488003 Msa0683660:intron 55.0%
TTCGGGAGGAAAGGGCTTTG+CGG + chr4_4:46488111-46488130 None:intergenic 55.0%
GGAAAGGAGGAAGGAGCAAG+TGG + chr4_4:46488148-46488167 None:intergenic 55.0%
TTGTGGGGACTGGGGGAAAT+TGG - chr4_4:46488403-46488422 Msa0683660:intron 55.0%
CTTCGCGTCGCGAAAGTCAC+CGG - chr4_4:46487190-46487209 Msa0683660:three_prime_UTR 60.0%
GAGGGCTGTGGAAAGGAGGA+AGG + chr4_4:46488157-46488176 None:intergenic 60.0%
AAGGGAGGGCTGTGGAAAGG+AGG + chr4_4:46488161-46488180 None:intergenic 60.0%
AGGAAGGGAGGGCTGTGGAA+AGG + chr4_4:46488164-46488183 None:intergenic 60.0%
TCTCAAGGAAGGGAGGGCTG+TGG + chr4_4:46488169-46488188 None:intergenic 60.0%
CTTCGCGTCGCGAAAGTCAC+CGG - chr4_4:46487190-46487209 Msa0683660:three_prime_UTR 60.0%
GAGGGCTGTGGAAAGGAGGA+AGG + chr4_4:46488157-46488176 None:intergenic 60.0%
AAGGGAGGGCTGTGGAAAGG+AGG + chr4_4:46488161-46488180 None:intergenic 60.0%
AGGAAGGGAGGGCTGTGGAA+AGG + chr4_4:46488164-46488183 None:intergenic 60.0%
TCTCAAGGAAGGGAGGGCTG+TGG + chr4_4:46488169-46488188 None:intergenic 60.0%
CGGTGACTTTCGCGACGCGA+AGG + chr4_4:46487192-46487211 None:intergenic 65.0%
CGGTGACTTTCGCGACGCGA+AGG + chr4_4:46487192-46487211 None:intergenic 65.0%
CGCGTCGCGAAAGTCACCGG+CGG - chr4_4:46487193-46487212 Msa0683660:three_prime_UTR 70.0%
!! GAGCTTATTGGGGGCGCCGC+CGG + chr4_4:46487212-46487231 None:intergenic 70.0%
CGCGTCGCGAAAGTCACCGG+CGG - chr4_4:46487193-46487212 Msa0683660:three_prime_UTR 70.0%
!! GAGCTTATTGGGGGCGCCGC+CGG + chr4_4:46487212-46487231 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 46486938 46489218 46486938 ID=Msa0683660;Name=Msa0683660
chr4_4 mRNA 46486938 46489218 46486938 ID=Msa0683660-mRNA-1;Parent=Msa0683660;Name=Msa0683660-mRNA-1;_AED=0.04;_eAED=0.04;_QI=55|1|1|1|1|1|4|339|122
chr4_4 exon 46486938 46487351 46486938 ID=Msa0683660-mRNA-1:exon:11442;Parent=Msa0683660-mRNA-1
chr4_4 exon 46488567 46488723 46488567 ID=Msa0683660-mRNA-1:exon:11441;Parent=Msa0683660-mRNA-1
chr4_4 exon 46488917 46489049 46488917 ID=Msa0683660-mRNA-1:exon:11440;Parent=Msa0683660-mRNA-1
chr4_4 exon 46489160 46489218 46489160 ID=Msa0683660-mRNA-1:exon:11439;Parent=Msa0683660-mRNA-1
chr4_4 five_prime_UTR 46489164 46489218 46489164 ID=Msa0683660-mRNA-1:five_prime_utr;Parent=Msa0683660-mRNA-1
chr4_4 CDS 46489160 46489163 46489160 ID=Msa0683660-mRNA-1:cds;Parent=Msa0683660-mRNA-1
chr4_4 CDS 46488917 46489049 46488917 ID=Msa0683660-mRNA-1:cds;Parent=Msa0683660-mRNA-1
chr4_4 CDS 46488567 46488723 46488567 ID=Msa0683660-mRNA-1:cds;Parent=Msa0683660-mRNA-1
chr4_4 CDS 46487277 46487351 46487277 ID=Msa0683660-mRNA-1:cds;Parent=Msa0683660-mRNA-1
chr4_4 three_prime_UTR 46486938 46487276 46486938 ID=Msa0683660-mRNA-1:three_prime_utr;Parent=Msa0683660-mRNA-1
Gene Sequence

>Msa0683660

ATGGCTATCAAGGTTTATGAGCTGAGGCAAAAAACAAAAGCAGATCTTCTCAATCAATTGAAAGATCTGAAAGCAGAACTTGCTCTCCTTCGCGTCGCGAAAGTCACCGGCGGCGCCCCCAATAAGCTCTCCAAGATCAAGGTTGTTCGGCTCAACATCGCTCAAGTTTTGACTGTGATATCACAGAAACAAAAGACAGCATTGAGGGAAGTTTATAAGGGGAAGAAGTACTTGCCTTTGGATCTCCGTCCCAAGAAGACGAGAGCTATTAGAAGACGCCTCACTAAGCATCAGCTCTCTTTGAAGACAGAGCGTGAGAAGAAGAAGGAAATCTACTTCCCAGTGAGAAAGTATGCCATTAAGGCGTAA
Protein sequence

>Msa0683660

MAIKVYELRQKTKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLNIAQVLTVISQKQKTALREVYKGKKYLPLDLRPKKTRAIRRRLTKHQLSLKTEREKKKEIYFPVRKYAIKA*