Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270730 | A0A072TLH5 | 96.203 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 1.02e-45 | 150 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002250 | Msa1270730 | 0.822383 | 2.438941e-53 | -8.615850e-47 |
| Msa0003630 | Msa1270730 | 0.845405 | 4.144347e-59 | -8.615850e-47 |
| Msa0004840 | Msa1270730 | 0.820377 | 7.084162e-53 | -8.615850e-47 |
| Msa0005270 | Msa1270730 | 0.864854 | 9.011754e-65 | -8.615850e-47 |
| Msa0006110 | Msa1270730 | 0.816850 | 4.469802e-52 | -8.615850e-47 |
| Msa0007440 | Msa1270730 | 0.815183 | 1.053561e-51 | -8.615850e-47 |
| Msa0007470 | Msa1270730 | 0.803688 | 3.094296e-49 | -8.615850e-47 |
| Msa0007990 | Msa1270730 | 0.810564 | 1.082555e-50 | -8.615850e-47 |
| Msa0008220 | Msa1270730 | 0.809264 | 2.062441e-50 | -8.615850e-47 |
| Msa0009790 | Msa1270730 | 0.835883 | 1.294934e-56 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1270730 | MtrunA17_Chr8g0341501 | 96.203 | 79 | 3 | 0 | 1 | 79 | 1 | 79 | 1.97e-49 | 150 |
Find 11 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAAAGAAGCTGAAAGAAA+TGG | 0.356123 | 8_3:+4053978 | Msa1270730:CDS |
| AGTTGAGGTGGTGTCTATGG+AGG | 0.609297 | 8_3:+4053954 | Msa1270730:CDS |
| ATGGATTCAAATAAGTTGAA+AGG | 0.552282 | 8_3:+4053997 | Msa1270730:CDS |
| CGTCAAGTTAGTGCTCGTTT+TGG | 0.356065 | 8_3:+4054060 | Msa1270730:CDS |
| CTCCTTCTTTAATTTGTGAA+TGG | 0.374793 | 8_3:-4053890 | None:intergenic |
| GAAAGGTGAGATCAAGAAAT+GGG | 0.482363 | 8_3:+4054014 | Msa1270730:CDS |
| GAGGTGGTGTCTATGGAGGA+AGG | 0.559344 | 8_3:+4053958 | Msa1270730:CDS |
| TACCATTCACAAATTAAAGA+AGG | 0.518100 | 8_3:+4053888 | Msa1270730:CDS |
| TGAAAGGTGAGATCAAGAAA+TGG | 0.464102 | 8_3:+4054013 | Msa1270730:CDS |
| TGAGATCAAGAAATGGGCTA+AGG | 0.607413 | 8_3:+4054020 | Msa1270730:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGGAAAGAAGCTGAAAGAAA+TGG | + | chr8_3:4053978-4053997 | Msa1270730:CDS | 35.0% | |
| TGAAAGGTGAGATCAAGAAA+TGG | + | chr8_3:4054013-4054032 | Msa1270730:CDS | 35.0% | |
| GAAAGGTGAGATCAAGAAAT+GGG | + | chr8_3:4054014-4054033 | Msa1270730:CDS | 35.0% | |
| TTCAGTTGAGGTGGTGTCTA+TGG | + | chr8_3:4053951-4053970 | Msa1270730:CDS | 45.0% | |
| AGTTGAGGTGGTGTCTATGG+AGG | + | chr8_3:4053954-4053973 | Msa1270730:CDS | 50.0% | |
| GAGGTGGTGTCTATGGAGGA+AGG | + | chr8_3:4053958-4053977 | Msa1270730:CDS | 55.0% | |
| ! | TACCATTCACAAATTAAAGA+AGG | + | chr8_3:4053888-4053907 | Msa1270730:CDS | 25.0% |
| ! | CGTCAAGTTAGTGCTCGTTT+TGG | + | chr8_3:4054060-4054079 | Msa1270730:CDS | 45.0% |
| !! | AAATCTCTATCTTTTAATGG+TGG | + | chr8_3:4053862-4053881 | Msa1270730:CDS | 25.0% |
| !! | ATGGATTCAAATAAGTTGAA+AGG | + | chr8_3:4053997-4054016 | Msa1270730:CDS | 25.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_3 | gene | 4053850 | 4054089 | 4053850 | ID=Msa1270730;Name=Msa1270730 |
| chr8_3 | mRNA | 4053850 | 4054089 | 4053850 | ID=Msa1270730-mRNA-1;Parent=Msa1270730;Name=Msa1270730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|79 |
| chr8_3 | exon | 4053850 | 4054089 | 4053850 | ID=Msa1270730-mRNA-1:exon:1301;Parent=Msa1270730-mRNA-1 |
| chr8_3 | CDS | 4053850 | 4054089 | 4053850 | ID=Msa1270730-mRNA-1:cds;Parent=Msa1270730-mRNA-1 |
| Gene Sequence |
>Msa1270730 ATGTCTGTGAAGAAATCTCTATCTTTTAATGGTGGAAGTACCATTCACAAATTAAAGAAGGAGAAATCTACAAAACGAAGACGTTTTGATCGTTGTTTTTCTTCAGTTGAGGTGGTGTCTATGGAGGAAGGAAAGAAGCTGAAAGAAATGGATTCAAATAAGTTGAAAGGTGAGATCAAGAAATGGGCTAAGGCTGTTGTTGCATATGCTCGTCAAGTTAGTGCTCGTTTTGGAAGCTAG |
| Protein sequence |
>Msa1270730 MSVKKSLSFNGGSTIHKLKKEKSTKRRRFDRCFSSVEVVSMEEGKKLKEMDSNKLKGEIKKWAKAVVAYARQVSARFGS* |
