AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192120 MtrunA17_Chr2g0282081 98.592 213 3 0 1 213 1 213 1.03e-154 427
Msa0192120 MtrunA17_Chr8g0377351 42.308 208 109 3 11 211 7 210 8.26e-49 159
Msa0192120 MtrunA17_Chr3g0122531 36.649 191 120 1 22 211 23 213 1.37e-36 127
Msa0192120 MtrunA17_Chr4g0047441 44.737 152 63 2 40 191 1 131 4.94e-34 118
Msa0192120 MtrunA17_Chr3g0122501 39.604 202 107 2 21 211 9 206 1.74e-32 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192120 AT3G05500.1 62.557 219 59 3 17 212 13 231 2.00e-91 268
Msa0192120 AT2G47780.1 47.396 192 101 0 20 211 36 227 1.50e-63 197
Msa0192120 AT3G05500.2 60.839 143 34 2 92 212 17 159 1.21e-51 165
Msa0192120 AT1G67360.1 39.899 198 112 2 21 211 9 206 5.03e-38 132
Msa0192120 AT1G67360.2 39.899 198 112 2 21 211 9 206 5.03e-38 132

Find 73 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGGTGGTGAGGGAATTT+AGG 0.106670 2_1:+7826585 None:intergenic
GTGATCGGTGAATTGATATT+AGG 0.185956 2_1:+7826489 None:intergenic
TTTGATATCATGTGAATAAA+TGG 0.226045 2_1:-7825468 Msa0192120:three_prime_UTR
TGAGGTGGTGAGGGAATTTA+GGG 0.255695 2_1:+7826586 None:intergenic
ACAGCACACTGCTCAGCTTT+TGG 0.302785 2_1:+7825911 None:intergenic
AGCTGAGCAGTGTGCTGTTT+CGG 0.305054 2_1:-7825906 Msa0192120:CDS
GGTGTTGTTGAATCTGCTTC+TGG 0.305257 2_1:-7825965 Msa0192120:CDS
TCACTACAATAAGCAGCTTT+TGG 0.309551 2_1:+7825827 None:intergenic
AGGGTTTGAAAAGGGAAAAT+TGG 0.318652 2_1:+7826515 None:intergenic
CGCAAGATTGGTATTGCATT+AGG 0.331896 2_1:+7825417 None:intergenic
CTGCTTAGTTGATATGATTT+CGG 0.361753 2_1:-7825706 Msa0192120:exon
CATGTGAATAAATGGATTAT+TGG 0.369399 2_1:-7825460 Msa0192120:three_prime_UTR
TGATCGGTGAATTGATATTA+GGG 0.370599 2_1:+7826490 None:intergenic
GGTGAATTGATATTAGGGTT+AGG 0.372007 2_1:+7826495 None:intergenic
TTGCGAATTGCACGAATTCA+AGG 0.379176 2_1:+7826260 None:intergenic
GGGTTTGAAAAGGGAAAATT+GGG 0.383745 2_1:+7826516 None:intergenic
GTGAATTGATATTAGGGTTA+GGG 0.390835 2_1:+7826496 None:intergenic
CCTCACATTGAGCGCAAGAT+TGG 0.396711 2_1:+7825405 None:intergenic
AGTCTATGGTAGTATGATAC+TGG 0.397279 2_1:-7825513 Msa0192120:three_prime_UTR
AAGATGAGTTGGGTTATATA+TGG 0.408114 2_1:+7826554 None:intergenic
TCTTCGACGGTGTCAACGCC+GGG 0.411977 2_1:+7826169 None:intergenic
CAATTGTTGTTGTTGTGGTT+CGG 0.426722 2_1:+7826458 None:intergenic
AGTTTCTGCTTATCTTCCAT+TGG 0.431002 2_1:-7825759 Msa0192120:CDS
GAAACCTTCTTGACGTTAGT+AGG 0.432023 2_1:+7826028 None:intergenic
GTGTCAACGCCGGGTTTCAA+TGG 0.442609 2_1:+7826178 None:intergenic
CGAACCACAACAACAACAAT+TGG 0.444881 2_1:-7826457 Msa0192120:intron
TGAGAAGCTCGACGGGGACT+TGG 0.446518 2_1:+7826104 None:intergenic
TTGTTGTGGTTCGGCGGCCA+TGG 0.446829 2_1:+7826467 None:intergenic
ATTAGGGTTAGGGTTTGAAA+AGG 0.453815 2_1:+7826506 None:intergenic
TTCTTCGACGGTGTCAACGC+CGG 0.458313 2_1:+7826168 None:intergenic
GAAAAGGGAAAATTGGGAAT+GGG 0.478733 2_1:+7826522 None:intergenic
AGCAGTGTGCTGTTTCGGCT+TGG 0.479724 2_1:-7825901 Msa0192120:CDS
TGGTGGAATTTGTCGTAGAC+TGG 0.480970 2_1:+7826124 None:intergenic
ACGTATTTGAGAAGCTCGAC+GGG 0.492555 2_1:+7826097 None:intergenic
GTGGATAGTGTGACTTTGAA+TGG 0.502511 2_1:-7825610 Msa0192120:three_prime_UTR
TCCTACGCTAAGGAACGTTC+CGG 0.516851 2_1:-7826202 Msa0192120:CDS
GAAGCTCGACGGGGACTTGG+TGG 0.519628 2_1:+7826107 None:intergenic
GAGAATCGCGCATCGCATAA+CGG 0.523618 2_1:+7826228 None:intergenic
TTAGGGTTAGGGTTTGAAAA+GGG 0.532905 2_1:+7826507 None:intergenic
ATCTTCTCAGTAGGAACCAA+TGG 0.534408 2_1:+7825743 None:intergenic
TATATATGGAAGAGGAAATG+AGG 0.535233 2_1:+7826568 None:intergenic
TGAAAAGGGAAAATTGGGAA+TGG 0.536602 2_1:+7826521 None:intergenic
AAGAGGAAATGAGGTGGTGA+GGG 0.537299 2_1:+7826577 None:intergenic
GTTGTTTCGAGTGCGGAGAA+AGG 0.541026 2_1:-7825788 Msa0192120:CDS
GGTTCGGCGGCCATGGTGAT+CGG 0.541124 2_1:+7826474 None:intergenic
TCGCGCATCGCATAACGGCT+TGG 0.544794 2_1:+7826233 None:intergenic
GAAGAGGAAATGAGGTGGTG+AGG 0.548134 2_1:+7826576 None:intergenic
GTCAGCGAAGTCCGTCGCAC+CGG 0.549076 2_1:-7825986 Msa0192120:CDS
AGTTGGGTTATATATGGAAG+AGG 0.555379 2_1:+7826560 None:intergenic
AGTACCAATTGTTGTTGTTG+TGG 0.556611 2_1:+7826453 None:intergenic
GTTTCCTACTGTTGCAAACG+TGG 0.564096 2_1:-7825855 Msa0192120:CDS
TGTTCCTACTAACGTCAAGA+AGG 0.568434 2_1:-7826032 Msa0192120:CDS
TGGATAGTGTGACTTTGAAT+GGG 0.568901 2_1:-7825609 Msa0192120:three_prime_UTR
ATGACGATCGATCTCAGAAA+CGG 0.570897 2_1:+7826054 None:intergenic
ATTCAACAACACCGGTGCGA+CGG 0.585997 2_1:+7825975 None:intergenic
CCAATCTTGCGCTCAATGTG+AGG 0.591238 2_1:-7825405 Msa0192120:three_prime_UTR
AACGCCGGGTTTCAATGGAC+CGG 0.601419 2_1:+7826183 None:intergenic
ACCTTAGCAATCTTCTCAGT+AGG 0.605822 2_1:+7825734 None:intergenic
TCCTACTGAGAAGATTGCTA+AGG 0.606990 2_1:-7825735 Msa0192120:CDS
GACGTATTTGAGAAGCTCGA+CGG 0.607045 2_1:+7826096 None:intergenic
CGTTCCGGTCCATTGAAACC+CGG 0.611239 2_1:-7826187 Msa0192120:CDS
GAAGCCGTTAAAACTGTCGT+TGG 0.611861 2_1:-7826148 Msa0192120:CDS
TGAAGCTGTTGTTTCGAGTG+CGG 0.612597 2_1:-7825795 Msa0192120:CDS
GATTCTCTATTCCTACGCTA+AGG 0.614531 2_1:-7826212 Msa0192120:CDS
AAAACCACGTTTGCAACAGT+AGG 0.643849 2_1:+7825851 None:intergenic
TTGTTGTTGTTGTGGTTCGG+CGG 0.643985 2_1:+7826461 None:intergenic
ACGGACTTCGCTGACGACAG+AGG 0.645739 2_1:+7825994 None:intergenic
TATCAATTCACCGATCACCA+TGG 0.660917 2_1:-7826484 Msa0192120:exon
TTTGCAACAGTAGGAAACAA+AGG 0.663535 2_1:+7825860 None:intergenic
AGAAGCAGATTCAACAACAC+CGG 0.667309 2_1:+7825967 None:intergenic
ATATGGAAGAGGAAATGAGG+TGG 0.704754 2_1:+7826571 None:intergenic
ACCGGAACGTTCCTTAGCGT+AGG 0.708340 2_1:+7826201 None:intergenic
CGTATTTGAGAAGCTCGACG+GGG 0.734758 2_1:+7826098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATGTTGAATGTTTAAT+TGG - chr2_1:7825712-7825731 Msa0192120:exon 15.0%
!!! ATTTAATTTGATTTGATTGA+AGG - chr2_1:7825764-7825783 Msa0192120:CDS 15.0%
!!! TTTTATTTTAAAACTGTTTC+TGG - chr2_1:7826462-7826481 Msa0192120:CDS 15.0%
!! TTATATGTTGAATGTTTAAT+TGG - chr2_1:7825712-7825731 Msa0192120:exon 15.0%
!!! ATTTAATTTGATTTGATTGA+AGG - chr2_1:7825764-7825783 Msa0192120:CDS 15.0%
!!! TTTTATTTTAAAACTGTTTC+TGG - chr2_1:7826462-7826481 Msa0192120:CDS 15.0%
!!! TTTTTAATCGTTATAGTCTA+TGG - chr2_1:7826569-7826588 Msa0192120:five_prime_UTR 20.0%
!! TTTGATATCATGTGAATAAA+TGG - chr2_1:7826628-7826647 Msa0192120:five_prime_UTR 20.0%
!!! TTTTTAATCGTTATAGTCTA+TGG - chr2_1:7826569-7826588 Msa0192120:five_prime_UTR 20.0%
!! TTTGATATCATGTGAATAAA+TGG - chr2_1:7826628-7826647 Msa0192120:five_prime_UTR 20.0%
!!! TCAGTTTTTCAATACTATAG+CGG + chr2_1:7825437-7825456 None:intergenic 25.0%
! GATTAAAAAACAGTAGAAAC+AGG + chr2_1:7826558-7826577 None:intergenic 25.0%
!!! CATGTGAATAAATGGATTAT+TGG - chr2_1:7826636-7826655 Msa0192120:five_prime_UTR 25.0%
!!! TCAGTTTTTCAATACTATAG+CGG + chr2_1:7825437-7825456 None:intergenic 25.0%
! GATTAAAAAACAGTAGAAAC+AGG + chr2_1:7826558-7826577 None:intergenic 25.0%
!!! CATGTGAATAAATGGATTAT+TGG - chr2_1:7826636-7826655 Msa0192120:five_prime_UTR 25.0%
TATATATGGAAGAGGAAATG+AGG + chr2_1:7825531-7825550 None:intergenic 30.0%
! AAGATGAGTTGGGTTATATA+TGG + chr2_1:7825545-7825564 None:intergenic 30.0%
GAAAAATGTGAAGATGAGTT+GGG + chr2_1:7825555-7825574 None:intergenic 30.0%
GTGAATTGATATTAGGGTTA+GGG + chr2_1:7825603-7825622 None:intergenic 30.0%
TGATCGGTGAATTGATATTA+GGG + chr2_1:7825609-7825628 None:intergenic 30.0%
! CTGCTTAGTTGATATGATTT+CGG - chr2_1:7826390-7826409 Msa0192120:intron 30.0%
!!! TTTTAAAACTGTTTCTGGTG+TGG - chr2_1:7826467-7826486 Msa0192120:CDS 30.0%
TATATATGGAAGAGGAAATG+AGG + chr2_1:7825531-7825550 None:intergenic 30.0%
! AAGATGAGTTGGGTTATATA+TGG + chr2_1:7825545-7825564 None:intergenic 30.0%
GAAAAATGTGAAGATGAGTT+GGG + chr2_1:7825555-7825574 None:intergenic 30.0%
GTGAATTGATATTAGGGTTA+GGG + chr2_1:7825603-7825622 None:intergenic 30.0%
TGATCGGTGAATTGATATTA+GGG + chr2_1:7825609-7825628 None:intergenic 30.0%
! CTGCTTAGTTGATATGATTT+CGG - chr2_1:7826390-7826409 Msa0192120:intron 30.0%
!!! TTTTAAAACTGTTTCTGGTG+TGG - chr2_1:7826467-7826486 Msa0192120:CDS 30.0%
AGTTGGGTTATATATGGAAG+AGG + chr2_1:7825539-7825558 None:intergenic 35.0%
GGAAAAATGTGAAGATGAGT+TGG + chr2_1:7825556-7825575 None:intergenic 35.0%
GAAAAGGGAAAATTGGGAAT+GGG + chr2_1:7825577-7825596 None:intergenic 35.0%
TGAAAAGGGAAAATTGGGAA+TGG + chr2_1:7825578-7825597 None:intergenic 35.0%
GGGTTTGAAAAGGGAAAATT+GGG + chr2_1:7825583-7825602 None:intergenic 35.0%
AGGGTTTGAAAAGGGAAAAT+TGG + chr2_1:7825584-7825603 None:intergenic 35.0%
TTAGGGTTAGGGTTTGAAAA+GGG + chr2_1:7825592-7825611 None:intergenic 35.0%
ATTAGGGTTAGGGTTTGAAA+AGG + chr2_1:7825593-7825612 None:intergenic 35.0%
GGTGAATTGATATTAGGGTT+AGG + chr2_1:7825604-7825623 None:intergenic 35.0%
GTGATCGGTGAATTGATATT+AGG + chr2_1:7825610-7825629 None:intergenic 35.0%
! CAATTGTTGTTGTTGTGGTT+CGG + chr2_1:7825641-7825660 None:intergenic 35.0%
! AGTACCAATTGTTGTTGTTG+TGG + chr2_1:7825646-7825665 None:intergenic 35.0%
TTTGCAACAGTAGGAAACAA+AGG + chr2_1:7826239-7826258 None:intergenic 35.0%
! TCACTACAATAAGCAGCTTT+TGG + chr2_1:7826272-7826291 None:intergenic 35.0%
AGTTTCTGCTTATCTTCCAT+TGG - chr2_1:7826337-7826356 Msa0192120:intron 35.0%
TGGATAGTGTGACTTTGAAT+GGG - chr2_1:7826487-7826506 Msa0192120:exon 35.0%
AGTCTATGGTAGTATGATAC+TGG - chr2_1:7826583-7826602 Msa0192120:five_prime_UTR 35.0%
AGTTGGGTTATATATGGAAG+AGG + chr2_1:7825539-7825558 None:intergenic 35.0%
GGAAAAATGTGAAGATGAGT+TGG + chr2_1:7825556-7825575 None:intergenic 35.0%
GAAAAGGGAAAATTGGGAAT+GGG + chr2_1:7825577-7825596 None:intergenic 35.0%
TGAAAAGGGAAAATTGGGAA+TGG + chr2_1:7825578-7825597 None:intergenic 35.0%
GGGTTTGAAAAGGGAAAATT+GGG + chr2_1:7825583-7825602 None:intergenic 35.0%
AGGGTTTGAAAAGGGAAAAT+TGG + chr2_1:7825584-7825603 None:intergenic 35.0%
TTAGGGTTAGGGTTTGAAAA+GGG + chr2_1:7825592-7825611 None:intergenic 35.0%
ATTAGGGTTAGGGTTTGAAA+AGG + chr2_1:7825593-7825612 None:intergenic 35.0%
GGTGAATTGATATTAGGGTT+AGG + chr2_1:7825604-7825623 None:intergenic 35.0%
GTGATCGGTGAATTGATATT+AGG + chr2_1:7825610-7825629 None:intergenic 35.0%
! CAATTGTTGTTGTTGTGGTT+CGG + chr2_1:7825641-7825660 None:intergenic 35.0%
! AGTACCAATTGTTGTTGTTG+TGG + chr2_1:7825646-7825665 None:intergenic 35.0%
TTTGCAACAGTAGGAAACAA+AGG + chr2_1:7826239-7826258 None:intergenic 35.0%
! TCACTACAATAAGCAGCTTT+TGG + chr2_1:7826272-7826291 None:intergenic 35.0%
AGTTTCTGCTTATCTTCCAT+TGG - chr2_1:7826337-7826356 Msa0192120:intron 35.0%
TGGATAGTGTGACTTTGAAT+GGG - chr2_1:7826487-7826506 Msa0192120:exon 35.0%
AGTCTATGGTAGTATGATAC+TGG - chr2_1:7826583-7826602 Msa0192120:five_prime_UTR 35.0%
TTAGAGAGAAAAACGAAGGG+TGG + chr2_1:7825490-7825509 None:intergenic 40.0%
GGGTTAGAGAGAAAAACGAA+GGG + chr2_1:7825493-7825512 None:intergenic 40.0%
AGGGTTAGAGAGAAAAACGA+AGG + chr2_1:7825494-7825513 None:intergenic 40.0%
ATATGGAAGAGGAAATGAGG+TGG + chr2_1:7825528-7825547 None:intergenic 40.0%
TATCAATTCACCGATCACCA+TGG - chr2_1:7825612-7825631 Msa0192120:three_prime_UTR 40.0%
CGAACCACAACAACAACAAT+TGG - chr2_1:7825639-7825658 Msa0192120:three_prime_UTR 40.0%
TTGCGAATTGCACGAATTCA+AGG + chr2_1:7825839-7825858 None:intergenic 40.0%
GATTCTCTATTCCTACGCTA+AGG - chr2_1:7825884-7825903 Msa0192120:CDS 40.0%
!! AGTTTTAACGGCTTCTTCGA+CGG + chr2_1:7825943-7825962 None:intergenic 40.0%
!! TGGTCCAACGACAGTTTTAA+CGG + chr2_1:7825955-7825974 None:intergenic 40.0%
ATGACGATCGATCTCAGAAA+CGG + chr2_1:7826045-7826064 None:intergenic 40.0%
TGTTCCTACTAACGTCAAGA+AGG - chr2_1:7826064-7826083 Msa0192120:CDS 40.0%
GAAACCTTCTTGACGTTAGT+AGG + chr2_1:7826071-7826090 None:intergenic 40.0%
AGAAGCAGATTCAACAACAC+CGG + chr2_1:7826132-7826151 None:intergenic 40.0%
AAAACCACGTTTGCAACAGT+AGG + chr2_1:7826248-7826267 None:intergenic 40.0%
ATCTTCTCAGTAGGAACCAA+TGG + chr2_1:7826356-7826375 None:intergenic 40.0%
TCCTACTGAGAAGATTGCTA+AGG - chr2_1:7826361-7826380 Msa0192120:intron 40.0%
ACCTTAGCAATCTTCTCAGT+AGG + chr2_1:7826365-7826384 None:intergenic 40.0%
GTGGATAGTGTGACTTTGAA+TGG - chr2_1:7826486-7826505 Msa0192120:exon 40.0%
CGCAAGATTGGTATTGCATT+AGG + chr2_1:7826682-7826701 None:intergenic 40.0%
TTAGAGAGAAAAACGAAGGG+TGG + chr2_1:7825490-7825509 None:intergenic 40.0%
GGGTTAGAGAGAAAAACGAA+GGG + chr2_1:7825493-7825512 None:intergenic 40.0%
AGGGTTAGAGAGAAAAACGA+AGG + chr2_1:7825494-7825513 None:intergenic 40.0%
ATATGGAAGAGGAAATGAGG+TGG + chr2_1:7825528-7825547 None:intergenic 40.0%
TATCAATTCACCGATCACCA+TGG - chr2_1:7825612-7825631 Msa0192120:three_prime_UTR 40.0%
CGAACCACAACAACAACAAT+TGG - chr2_1:7825639-7825658 Msa0192120:three_prime_UTR 40.0%
TTGCGAATTGCACGAATTCA+AGG + chr2_1:7825839-7825858 None:intergenic 40.0%
GATTCTCTATTCCTACGCTA+AGG - chr2_1:7825884-7825903 Msa0192120:CDS 40.0%
!! AGTTTTAACGGCTTCTTCGA+CGG + chr2_1:7825943-7825962 None:intergenic 40.0%
!! TGGTCCAACGACAGTTTTAA+CGG + chr2_1:7825955-7825974 None:intergenic 40.0%
ATGACGATCGATCTCAGAAA+CGG + chr2_1:7826045-7826064 None:intergenic 40.0%
TGTTCCTACTAACGTCAAGA+AGG - chr2_1:7826064-7826083 Msa0192120:CDS 40.0%
GAAACCTTCTTGACGTTAGT+AGG + chr2_1:7826071-7826090 None:intergenic 40.0%
AGAAGCAGATTCAACAACAC+CGG + chr2_1:7826132-7826151 None:intergenic 40.0%
AAAACCACGTTTGCAACAGT+AGG + chr2_1:7826248-7826267 None:intergenic 40.0%
ATCTTCTCAGTAGGAACCAA+TGG + chr2_1:7826356-7826375 None:intergenic 40.0%
TCCTACTGAGAAGATTGCTA+AGG - chr2_1:7826361-7826380 Msa0192120:intron 40.0%
ACCTTAGCAATCTTCTCAGT+AGG + chr2_1:7826365-7826384 None:intergenic 40.0%
GTGGATAGTGTGACTTTGAA+TGG - chr2_1:7826486-7826505 Msa0192120:exon 40.0%
CGCAAGATTGGTATTGCATT+AGG + chr2_1:7826682-7826701 None:intergenic 40.0%
TGAAAAACTGAGACGCGTGT+TGG - chr2_1:7825446-7825465 Msa0192120:three_prime_UTR 45.0%
TGAGGTGGTGAGGGAATTTA+GGG + chr2_1:7825513-7825532 None:intergenic 45.0%
ATGAGGTGGTGAGGGAATTT+AGG + chr2_1:7825514-7825533 None:intergenic 45.0%
AAGAGGAAATGAGGTGGTGA+GGG + chr2_1:7825522-7825541 None:intergenic 45.0%
! TTGTTGTTGTTGTGGTTCGG+CGG + chr2_1:7825638-7825657 None:intergenic 45.0%
GAAGCCGTTAAAACTGTCGT+TGG - chr2_1:7825948-7825967 Msa0192120:CDS 45.0%
TGGTGGAATTTGTCGTAGAC+TGG + chr2_1:7825975-7825994 None:intergenic 45.0%
ACGTATTTGAGAAGCTCGAC+GGG + chr2_1:7826002-7826021 None:intergenic 45.0%
GACGTATTTGAGAAGCTCGA+CGG + chr2_1:7826003-7826022 None:intergenic 45.0%
!! GGTGTTGTTGAATCTGCTTC+TGG - chr2_1:7826131-7826150 Msa0192120:CDS 45.0%
GTTTCCTACTGTTGCAAACG+TGG - chr2_1:7826241-7826260 Msa0192120:CDS 45.0%
!! TGAAGCTGTTGTTTCGAGTG+CGG - chr2_1:7826301-7826320 Msa0192120:CDS 45.0%
TGAAAAACTGAGACGCGTGT+TGG - chr2_1:7825446-7825465 Msa0192120:three_prime_UTR 45.0%
TGAGGTGGTGAGGGAATTTA+GGG + chr2_1:7825513-7825532 None:intergenic 45.0%
ATGAGGTGGTGAGGGAATTT+AGG + chr2_1:7825514-7825533 None:intergenic 45.0%
AAGAGGAAATGAGGTGGTGA+GGG + chr2_1:7825522-7825541 None:intergenic 45.0%
! TTGTTGTTGTTGTGGTTCGG+CGG + chr2_1:7825638-7825657 None:intergenic 45.0%
GAAGCCGTTAAAACTGTCGT+TGG - chr2_1:7825948-7825967 Msa0192120:CDS 45.0%
TGGTGGAATTTGTCGTAGAC+TGG + chr2_1:7825975-7825994 None:intergenic 45.0%
ACGTATTTGAGAAGCTCGAC+GGG + chr2_1:7826002-7826021 None:intergenic 45.0%
GACGTATTTGAGAAGCTCGA+CGG + chr2_1:7826003-7826022 None:intergenic 45.0%
!! GGTGTTGTTGAATCTGCTTC+TGG - chr2_1:7826131-7826150 Msa0192120:CDS 45.0%
GTTTCCTACTGTTGCAAACG+TGG - chr2_1:7826241-7826260 Msa0192120:CDS 45.0%
!! TGAAGCTGTTGTTTCGAGTG+CGG - chr2_1:7826301-7826320 Msa0192120:CDS 45.0%
GAAGAGGAAATGAGGTGGTG+AGG + chr2_1:7825523-7825542 None:intergenic 50.0%
GAGAATCGCGCATCGCATAA+CGG + chr2_1:7825871-7825890 None:intergenic 50.0%
TCCTACGCTAAGGAACGTTC+CGG - chr2_1:7825894-7825913 Msa0192120:CDS 50.0%
CGTATTTGAGAAGCTCGACG+GGG + chr2_1:7826001-7826020 None:intergenic 50.0%
ATTCAACAACACCGGTGCGA+CGG + chr2_1:7826124-7826143 None:intergenic 50.0%
! ACAGCACACTGCTCAGCTTT+TGG + chr2_1:7826188-7826207 None:intergenic 50.0%
!! AGCTGAGCAGTGTGCTGTTT+CGG - chr2_1:7826190-7826209 Msa0192120:CDS 50.0%
! GTTGTTTCGAGTGCGGAGAA+AGG - chr2_1:7826308-7826327 Msa0192120:CDS 50.0%
CCTCACATTGAGCGCAAGAT+TGG + chr2_1:7826694-7826713 None:intergenic 50.0%
CCAATCTTGCGCTCAATGTG+AGG - chr2_1:7826691-7826710 Msa0192120:five_prime_UTR 50.0%
GAAGAGGAAATGAGGTGGTG+AGG + chr2_1:7825523-7825542 None:intergenic 50.0%
GAGAATCGCGCATCGCATAA+CGG + chr2_1:7825871-7825890 None:intergenic 50.0%
TCCTACGCTAAGGAACGTTC+CGG - chr2_1:7825894-7825913 Msa0192120:CDS 50.0%
CGTATTTGAGAAGCTCGACG+GGG + chr2_1:7826001-7826020 None:intergenic 50.0%
ATTCAACAACACCGGTGCGA+CGG + chr2_1:7826124-7826143 None:intergenic 50.0%
! ACAGCACACTGCTCAGCTTT+TGG + chr2_1:7826188-7826207 None:intergenic 50.0%
!! AGCTGAGCAGTGTGCTGTTT+CGG - chr2_1:7826190-7826209 Msa0192120:CDS 50.0%
! GTTGTTTCGAGTGCGGAGAA+AGG - chr2_1:7826308-7826327 Msa0192120:CDS 50.0%
CCTCACATTGAGCGCAAGAT+TGG + chr2_1:7826694-7826713 None:intergenic 50.0%
ACCGGAACGTTCCTTAGCGT+AGG + chr2_1:7825898-7825917 None:intergenic 55.0%
CGTTCCGGTCCATTGAAACC+CGG - chr2_1:7825909-7825928 Msa0192120:CDS 55.0%
AACGCCGGGTTTCAATGGAC+CGG + chr2_1:7825916-7825935 None:intergenic 55.0%
GTGTCAACGCCGGGTTTCAA+TGG + chr2_1:7825921-7825940 None:intergenic 55.0%
! TTCTTCGACGGTGTCAACGC+CGG + chr2_1:7825931-7825950 None:intergenic 55.0%
!! AGCAGTGTGCTGTTTCGGCT+TGG - chr2_1:7826195-7826214 Msa0192120:CDS 55.0%
ACCGGAACGTTCCTTAGCGT+AGG + chr2_1:7825898-7825917 None:intergenic 55.0%
CGTTCCGGTCCATTGAAACC+CGG - chr2_1:7825909-7825928 Msa0192120:CDS 55.0%
AACGCCGGGTTTCAATGGAC+CGG + chr2_1:7825916-7825935 None:intergenic 55.0%
GTGTCAACGCCGGGTTTCAA+TGG + chr2_1:7825921-7825940 None:intergenic 55.0%
! TTCTTCGACGGTGTCAACGC+CGG + chr2_1:7825931-7825950 None:intergenic 55.0%
!! AGCAGTGTGCTGTTTCGGCT+TGG - chr2_1:7826195-7826214 Msa0192120:CDS 55.0%
! TTGTTGTGGTTCGGCGGCCA+TGG + chr2_1:7825632-7825651 None:intergenic 60.0%
TCGCGCATCGCATAACGGCT+TGG + chr2_1:7825866-7825885 None:intergenic 60.0%
! TCTTCGACGGTGTCAACGCC+GGG + chr2_1:7825930-7825949 None:intergenic 60.0%
TGAGAAGCTCGACGGGGACT+TGG + chr2_1:7825995-7826014 None:intergenic 60.0%
ACGGACTTCGCTGACGACAG+AGG + chr2_1:7826105-7826124 None:intergenic 60.0%
! TTGTTGTGGTTCGGCGGCCA+TGG + chr2_1:7825632-7825651 None:intergenic 60.0%
TCGCGCATCGCATAACGGCT+TGG + chr2_1:7825866-7825885 None:intergenic 60.0%
! TCTTCGACGGTGTCAACGCC+GGG + chr2_1:7825930-7825949 None:intergenic 60.0%
TGAGAAGCTCGACGGGGACT+TGG + chr2_1:7825995-7826014 None:intergenic 60.0%
ACGGACTTCGCTGACGACAG+AGG + chr2_1:7826105-7826124 None:intergenic 60.0%
GGTTCGGCGGCCATGGTGAT+CGG + chr2_1:7825625-7825644 None:intergenic 65.0%
GAAGCTCGACGGGGACTTGG+TGG + chr2_1:7825992-7826011 None:intergenic 65.0%
GTCAGCGAAGTCCGTCGCAC+CGG - chr2_1:7826110-7826129 Msa0192120:CDS 65.0%
GGTTCGGCGGCCATGGTGAT+CGG + chr2_1:7825625-7825644 None:intergenic 65.0%
GAAGCTCGACGGGGACTTGG+TGG + chr2_1:7825992-7826011 None:intergenic 65.0%
GTCAGCGAAGTCCGTCGCAC+CGG - chr2_1:7826110-7826129 Msa0192120:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 7825405 7826713 7825405 ID=Msa0192120;Name=Msa0192120
chr2_1 mRNA 7825405 7826713 7825405 ID=Msa0192120-mRNA-1;Parent=Msa0192120;Name=Msa0192120-mRNA-1;_AED=0.00;_eAED=0.00;_QI=226|1|1|1|1|1|2|316|213
chr2_1 exon 7825405 7826332 7825405 ID=Msa0192120-mRNA-1:exon:3634;Parent=Msa0192120-mRNA-1
chr2_1 exon 7826458 7826713 7826458 ID=Msa0192120-mRNA-1:exon:3633;Parent=Msa0192120-mRNA-1
chr2_1 five_prime_UTR 7826488 7826713 7826488 ID=Msa0192120-mRNA-1:five_prime_utr;Parent=Msa0192120-mRNA-1
chr2_1 CDS 7826458 7826487 7826458 ID=Msa0192120-mRNA-1:cds;Parent=Msa0192120-mRNA-1
chr2_1 CDS 7825721 7826332 7825721 ID=Msa0192120-mRNA-1:cds;Parent=Msa0192120-mRNA-1
chr2_1 three_prime_UTR 7825405 7825720 7825405 ID=Msa0192120-mRNA-1:three_prime_utr;Parent=Msa0192120-mRNA-1
Gene Sequence

>Msa0192120

ATGGCCGCCGAACCACAACAACAACAATTGGTTGATGAGAACAATCGCGATCACACTGAAGAAGAGCAAGAATTGAAGTACCTTGAATTCGTGCAATTCGCAACGATCCAAGCCGTTATGCGATGCGCGATTCTCTATTCCTACGCTAAGGAACGTTCCGGTCCATTGAAACCCGGCGTTGACACCGTCGAAGAAGCCGTTAAAACTGTCGTTGGACCAGTCTACGACAAATTCCACCAAGTCCCCGTCGAGCTTCTCAAATACGTCGATCGCAAAGTCGACGAATCCGTTTCTGAGATCGATCGTCATGTTCCTACTAACGTCAAGAAGGTTTCAAATCAAGCTGCCTCTGTCGTCAGCGAAGTCCGTCGCACCGGTGTTGTTGAATCTGCTTCTGGACTTGCGAAAACTGTTTACTCGAAATATGAACCAAAAGCTGAGCAGTGTGCTGTTTCGGCTTGGAAAAAGTTGAATCAGCTTCCTTTGTTTCCTACTGTTGCAAACGTGGTTTTGCCAAAAGCTGCTTATTGTAGTGAGAAGTATAATGAAGCTGTTGTTTCGAGTGCGGAGAAAGGTTATAGAGTTTCTGCTTATCTTCCATTGGTTCCTACTGAGAAGATTGCTAAGGTGTTTGCTGCTTAG
Protein sequence

>Msa0192120

MAAEPQQQQLVDENNRDHTEEEQELKYLEFVQFATIQAVMRCAILYSYAKERSGPLKPGVDTVEEAVKTVVGPVYDKFHQVPVELLKYVDRKVDESVSEIDRHVPTNVKKVSNQAASVVSEVRRTGVVESASGLAKTVYSKYEPKAEQCAVSAWKKLNQLPLFPTVANVVLPKAAYCSEKYNEAVVSSAEKGYRVSAYLPLVPTEKIAKVFAA*