AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0217110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217110 MtrunA17_Chr2g0318901 94.636 261 14 0 1 261 13 273 0.0 529
Msa0217110 MtrunA17_Chr2g0318871 82.759 261 45 0 1 261 13 273 4.03e-169 468
Msa0217110 MtrunA17_Chr2g0318931 64.453 256 83 4 1 249 18 272 3.70e-119 342
Msa0217110 MtrunA17_Chr8g0355861 46.122 245 120 5 11 248 22 261 4.13e-77 234
Msa0217110 MtrunA17_Chr8g0355321 38.462 299 121 5 8 248 10 303 4.03e-75 231
Msa0217110 MtrunA17_Chr1g0168781 43.411 258 133 5 3 253 14 265 1.36e-71 220
Msa0217110 MtrunA17_Chr8g0355871 45.918 196 97 3 59 247 29 222 1.20e-60 191
Msa0217110 MtrunA17_Chr8g0355841 53.797 158 72 1 99 256 362 518 3.83e-58 193
Msa0217110 MtrunA17_Chr8g0355311 48.824 170 80 2 84 253 34 196 2.30e-57 182
Msa0217110 MtrunA17_Chr8g0355331 45.882 170 85 2 85 254 266 428 4.11e-54 180
Msa0217110 MtrunA17_Chr8g0355831 55.333 150 66 1 99 248 204 352 5.08e-52 173
Msa0217110 MtrunA17_Chr8g0355831 29.457 129 72 3 8 121 18 142 7.91e-11 61.6
Msa0217110 MtrunA17_Chr3g0139431 31.392 309 138 11 8 247 18 321 1.51e-42 147
Msa0217110 MtrunA17_Chr1g0147261 32.646 291 145 12 1 245 12 297 1.56e-40 141
Msa0217110 MtrunA17_Chr1g0147311 28.527 319 153 9 1 246 38 354 4.33e-37 134
Msa0217110 MtrunA17_Chr1g0147331 29.524 315 146 11 3 246 14 323 4.53e-36 130
Msa0217110 MtrunA17_Chr1g0146631 29.206 315 147 12 3 246 14 323 7.43e-35 127
Msa0217110 MtrunA17_Chr1g0147241 28.904 301 156 11 1 245 12 310 3.65e-33 122
Msa0217110 MtrunA17_Chr1g0147291 32.895 228 125 9 45 246 40 265 5.18e-31 115
Msa0217110 MtrunA17_Chr1g0147301 33.047 233 122 10 45 246 54 283 5.56e-31 116
Msa0217110 MtrunA17_Chr1g0147271 34.579 214 111 7 61 246 171 383 5.78e-30 115
Msa0217110 MtrunA17_Chr1g0147321 32.883 222 121 9 50 245 16 235 1.15e-29 112
Msa0217110 MtrunA17_Chr1g0147251 31.532 222 119 6 51 245 25 240 2.00e-29 111
Msa0217110 MtrunA17_Chr3g0144421 35.461 141 82 5 116 247 162 302 1.56e-19 85.9
Msa0217110 MtrunA17_Chr4g0034821 29.778 225 116 11 61 249 75 293 3.29e-18 82.4
Msa0217110 MtrunA17_Chr4g0034731 34.965 143 81 6 117 247 290 432 1.36e-15 75.9
Msa0217110 MtrunA17_Chr3g0138231 31.250 144 89 4 116 249 125 268 1.61e-15 74.3
Msa0217110 MtrunA17_Chr4g0034721 34.965 143 81 6 117 247 434 576 5.46e-15 74.3
Msa0217110 MtrunA17_Chr3g0144451 32.639 144 87 5 116 249 134 277 1.03e-14 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0217110 AT5G25610.1 33.036 224 121 8 51 245 166 389 1.27e-28 112
Msa0217110 AT1G49320.1 33.784 148 83 5 114 246 131 278 6.69e-16 75.9

Find 60 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTCATTTCCTTGGAAATA+AGG 0.224770 2_1:+63680153 Msa0217110:CDS
ACAAAGATTGTGCCTCTTTA+GGG 0.230864 2_1:-63680365 None:intergenic
AACAAAGATTGTGCCTCTTT+AGG 0.250870 2_1:-63680366 None:intergenic
GTCTTATCAATGATAATTTC+AGG 0.264308 2_1:+63680494 Msa0217110:three_prime_UTR
AAAATCCTTGGGAACATTAA+TGG 0.270156 2_1:+63679820 Msa0217110:CDS
CGTCCCTTTGCACAACCTTA+TGG 0.298036 2_1:+63679689 Msa0217110:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.300262 2_1:+63677329 Msa0217110:CDS
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 2_1:+63680216 Msa0217110:CDS
AAATCCTTGGGAACATTAAT+GGG 0.325478 2_1:+63679821 Msa0217110:CDS
GCTTCCCAATATTCTTCTTC+AGG 0.355894 2_1:-63677334 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 2_1:+63677341 Msa0217110:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 2_1:-63680190 None:intergenic
GTTTGTCATGCTGATACTTC+AGG 0.376770 2_1:+63680073 Msa0217110:CDS
GGATTTGCAATTTCTAAGCT+TGG 0.378778 2_1:+63679842 Msa0217110:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.385982 2_1:+63677292 Msa0217110:intron
TTGTTCATGTTTATCTATGA+AGG 0.411973 2_1:-63679891 None:intergenic
ACTGCCTGAAGAAGAATATT+GGG 0.412787 2_1:+63677330 Msa0217110:CDS
TATGAACATGAACTTTATCC+AGG 0.415715 2_1:+63679638 Msa0217110:CDS
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 2_1:+63679920 Msa0217110:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 2_1:+63680183 Msa0217110:CDS
AGAAAGCAGTTATGTGTCAT+AGG 0.437124 2_1:+63679945 Msa0217110:CDS
AAATAACAACCTTGAGGTCC+AGG 0.452077 2_1:-63677406 None:intergenic
AAACCTGTATGTCATTTCCT+TGG 0.455480 2_1:+63680145 Msa0217110:CDS
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 2_1:+63680322 Msa0217110:three_prime_UTR
TAGAAGTTCTAACAGAGAAG+TGG 0.468544 2_1:-63677380 None:intergenic
AACCTTATGGTGTTTATACA+TGG 0.471062 2_1:+63679702 Msa0217110:CDS
CTTCTTCAGGCAGTGATGCA+TGG 0.473820 2_1:-63677321 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.474967 2_1:-63677364 None:intergenic
TTGTGTGTCATCAATTTCCA+TGG 0.477747 2_1:-63679597 None:intergenic
GTTCCATCATTTGCCACCAA+TGG 0.478916 2_1:-63680031 None:intergenic
ACACCATAAGGTTGTGCAAA+GGG 0.481077 2_1:-63679692 None:intergenic
TTTCCAAGGAAATGACATAC+AGG 0.483607 2_1:-63680148 None:intergenic
CTGTTAGAACTTCTAAAGCC+TGG 0.484254 2_1:+63677388 Msa0217110:CDS
AGAAGTGGGAATGGGAGTGT+TGG 0.493399 2_1:-63677365 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 2_1:+63680196 Msa0217110:CDS
GGAGTACTCTCCATCATGGT+TGG 0.497653 2_1:+63680237 Msa0217110:exon
ATCCATGTATAAACACCATA+AGG 0.501910 2_1:-63679704 None:intergenic
GTTCCATTGGTGGCAAATGA+TGG 0.508291 2_1:+63680028 Msa0217110:CDS
CAGATTATGAAAGCTGATCC+TGG 0.513741 2_1:+63680118 Msa0217110:CDS
TTCTAACAGAGAAGTGGGAA+TGG 0.520149 2_1:-63677374 None:intergenic
ATAAAAGAGCCTTATTTCCA+AGG 0.523232 2_1:-63680162 None:intergenic
AGAAGTTCTAACAGAGAAGT+GGG 0.537078 2_1:-63677379 None:intergenic
TGTGTGTCATCAATTTCCAT+GGG 0.545618 2_1:-63679596 None:intergenic
TTTAGGAGTACTCTCCATCA+TGG 0.550450 2_1:+63680233 Msa0217110:exon
TCTAACAGAGAAGTGGGAAT+GGG 0.553253 2_1:-63677373 None:intergenic
CTTCTAAAGCCTGGACCTCA+AGG 0.560362 2_1:+63677397 Msa0217110:CDS
AACAGCAACATTGGCACCAT+AGG 0.567015 2_1:-63680212 None:intergenic
AAATCCCATTAATGTTCCCA+AGG 0.567393 2_1:-63679825 None:intergenic
GTAAAGAAAGCAGCTGCAGA+AGG 0.576977 2_1:+63679779 Msa0217110:CDS
ATTTATGTTACACAGTCCCA+TGG 0.579361 2_1:-63680265 None:intergenic
ATCATGGTTGGACACACCAT+GGG 0.579868 2_1:+63680249 Msa0217110:three_prime_UTR
GTACTCCTAAACAGCAACAT+TGG 0.581094 2_1:-63680221 None:intergenic
CATCATGGTTGGACACACCA+TGG 0.594647 2_1:+63680248 Msa0217110:three_prime_UTR
CATGGTGTGTCCAACCATGA+TGG 0.616911 2_1:-63680247 None:intergenic
TGAAATTAATGACCTCCCCA+TGG 0.617419 2_1:+63679580 Msa0217110:CDS
TGTCATCAATTTCCATGGGG+AGG 0.625070 2_1:-63679592 None:intergenic
TGACATACAGGTTTACTACC+AGG 0.625667 2_1:-63680136 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 2_1:-63679693 None:intergenic
GTGCAGCTTGCACTTGCAGG+AGG 0.696243 2_1:+63677295 Msa0217110:intron
GTGTGTCATCAATTTCCATG+GGG 0.722357 2_1:-63679595 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATCCTTTATTAATTTTAT+TGG + chr2_1:63677952-63677971 Msa0217110:intron 10.0%
!! TTAAATTGAAAAACTAATAA+GGG - chr2_1:63678011-63678030 None:intergenic 10.0%
!! ATTAAATTGAAAAACTAATA+AGG - chr2_1:63678012-63678031 None:intergenic 10.0%
!! TAAGATAAAACTTATATATT+TGG - chr2_1:63678349-63678368 None:intergenic 10.0%
!! ATAATTTACAAAGAAAAAAT+AGG - chr2_1:63679486-63679505 None:intergenic 10.0%
!!! TATTTTTTCTTTGTAAATTA+TGG + chr2_1:63679485-63679504 Msa0217110:intron 10.0%
!!! ATGTTTTAATATAATGAAAA+TGG + chr2_1:63680406-63680425 Msa0217110:three_prime_UTR 10.0%
!! TGATCCAATAAAATTAATAA+AGG - chr2_1:63677959-63677978 None:intergenic 15.0%
!! TAAATTGAAAAACTAATAAG+GGG - chr2_1:63678010-63678029 None:intergenic 15.0%
!! TATTTGTCATATAAATTGAA+TGG - chr2_1:63678182-63678201 None:intergenic 15.0%
!! TTATATGACAAATAATTCTA+AGG + chr2_1:63678188-63678207 Msa0217110:intron 15.0%
!! TATATGACAAATAATTCTAA+GGG + chr2_1:63678189-63678208 Msa0217110:intron 15.0%
!! TTTCTTAATTTATGTCTTAA+GGG - chr2_1:63678326-63678345 None:intergenic 15.0%
!!! GAATTTTAACTAATAAAAGA+TGG - chr2_1:63679043-63679062 None:intergenic 15.0%
!! TTTATTAGTTAAAATTCAGA+TGG + chr2_1:63679046-63679065 Msa0217110:intron 15.0%
!! TTATTAGTTAAAATTCAGAT+GGG + chr2_1:63679047-63679066 Msa0217110:intron 15.0%
!!! ATTTTGCTTATATTTTGAAA+CGG - chr2_1:63679254-63679273 None:intergenic 15.0%
!! AAAAAAAAATATCTACAATG+AGG - chr2_1:63680439-63680458 None:intergenic 15.0%
!! AAAATTAAATAACAACCTTG+AGG - chr2_1:63677415-63677434 None:intergenic 20.0%
!!! TCAAGGTTGTTATTTAATTT+TGG + chr2_1:63677414-63677433 Msa0217110:intron 20.0%
!! TAGTACCTATATTAATTAAC+AGG + chr2_1:63677505-63677524 Msa0217110:intron 20.0%
!! AGTACCTATATTAATTAACA+GGG + chr2_1:63677506-63677525 Msa0217110:intron 20.0%
!! GTTGAATCACAATATTAATT+AGG - chr2_1:63677702-63677721 None:intergenic 20.0%
!!! TCAGAAAAGGTAAATTTTAT+GGG + chr2_1:63677744-63677763 Msa0217110:intron 20.0%
!!! GGATTACTAATTCTTTTTTT+TGG - chr2_1:63677938-63677957 None:intergenic 20.0%
!!! AATTTATTTGTTAGGATGAA+AGG - chr2_1:63678283-63678302 None:intergenic 20.0%
!! TCATCCTAACAAATAAATTA+AGG + chr2_1:63678284-63678303 Msa0217110:intron 20.0%
!! CATCCTAACAAATAAATTAA+GGG + chr2_1:63678285-63678304 Msa0217110:intron 20.0%
!! TTTCCCTTAATTTATTTGTT+AGG - chr2_1:63678291-63678310 None:intergenic 20.0%
!! GTTTCTTAATTTATGTCTTA+AGG - chr2_1:63678327-63678346 None:intergenic 20.0%
!! GGCAAACATTATTAATTTAT+GGG - chr2_1:63678487-63678506 None:intergenic 20.0%
!! TGGCAAACATTATTAATTTA+TGG - chr2_1:63678488-63678507 None:intergenic 20.0%
!! TCTCTCAAGTTATATATTAT+TGG - chr2_1:63678508-63678527 None:intergenic 20.0%
!! ATAAAAGATGGAAAATTCTA+TGG - chr2_1:63679031-63679050 None:intergenic 20.0%
!!! ATAAGCAAAATTCACTTTTT+AGG + chr2_1:63679157-63679176 Msa0217110:intron 20.0%
!!! ATAAGCAAAATTCACTTTTT+AGG - chr2_1:63679157-63679176 None:intergenic 20.0%
!! AGAAAAAATAGGTATTTGAA+CGG - chr2_1:63679475-63679494 None:intergenic 20.0%
!!! TATTTTAAACCCATCTATTA+TGG + chr2_1:63679548-63679567 Msa0217110:intron 20.0%
!!! GTTCATAGAAAAATGTTTTT+GGG - chr2_1:63679625-63679644 None:intergenic 20.0%
!!! TGTTCATAGAAAAATGTTTT+TGG - chr2_1:63679626-63679645 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_1:63679734-63679753 Msa0217110:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_1:63679989-63680008 Msa0217110:CDS 20.0%
!!! ACTAAATTTTTAGTCTCTAC+AGG - chr2_1:63677489-63677508 None:intergenic 25.0%
! AAGACCCTGTTAATTAATAT+AGG - chr2_1:63677513-63677532 None:intergenic 25.0%
!! AAGATATGGTTTCTTTTTGT+GGG - chr2_1:63677570-63677589 None:intergenic 25.0%
!! AAATGTAACAACTTTTGCAT+TGG - chr2_1:63677638-63677657 None:intergenic 25.0%
! GTAAGAAATAAACTCAGAAA+AGG + chr2_1:63677731-63677750 Msa0217110:intron 25.0%
!! CTCAGAAAAGGTAAATTTTA+TGG + chr2_1:63677743-63677762 Msa0217110:intron 25.0%
! AGAGATGAAAAACTAATAAG+GGG - chr2_1:63678055-63678074 None:intergenic 25.0%
! CAGAGATGAAAAACTAATAA+GGG - chr2_1:63678056-63678075 None:intergenic 25.0%
! TCAGAGATGAAAAACTAATA+AGG - chr2_1:63678057-63678076 None:intergenic 25.0%
!! GAGAGATGTTATGATTTTTA+TGG + chr2_1:63678524-63678543 Msa0217110:intron 25.0%
! AAGATAGTGTGTTGAAAAAT+GGG - chr2_1:63678591-63678610 None:intergenic 25.0%
! AAAGATAGTGTGTTGAAAAA+TGG - chr2_1:63678592-63678611 None:intergenic 25.0%
!! TTTCAACACACTATCTTTTA+TGG + chr2_1:63678594-63678613 Msa0217110:intron 25.0%
!! TTCAACACACTATCTTTTAT+GGG + chr2_1:63678595-63678614 Msa0217110:intron 25.0%
! TGTTACACTTAAAACAAAGT+AGG + chr2_1:63678848-63678867 Msa0217110:intron 25.0%
!!! GAAGGAGTATTCTTTTTTAT+TGG - chr2_1:63679126-63679145 None:intergenic 25.0%
! CATTCATTTAATGATGTATG+TGG + chr2_1:63679182-63679201 Msa0217110:intron 25.0%
! CATTCATTTAATGATGTATG+TGG - chr2_1:63679182-63679201 None:intergenic 25.0%
!!! TTGCTTATATTTTGAAACGA+AGG + chr2_1:63679257-63679276 Msa0217110:intron 25.0%
!!! TGCTTATATTTTGAAACGAA+GGG + chr2_1:63679258-63679277 Msa0217110:intron 25.0%
! TCACAAAAATTGTCATCAAA+TGG - chr2_1:63679396-63679415 None:intergenic 25.0%
!! AAAATTTTGTGCAAAATCCT+TGG + chr2_1:63679808-63679827 Msa0217110:CDS 25.0%
!! AAATTTTGTGCAAAATCCTT+GGG + chr2_1:63679809-63679828 Msa0217110:CDS 25.0%
! TTGTTCATGTTTATCTATGA+AGG - chr2_1:63679894-63679913 None:intergenic 25.0%
!!! TCATAGGTTGAATTTTCAAA+AGG + chr2_1:63679961-63679980 Msa0217110:CDS 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_1:63680456-63680475 Msa0217110:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_1:63680457-63680476 Msa0217110:three_prime_UTR 25.0%
!!! TTTTTAGGGGTAATTATGTT+TGG + chr2_1:63680471-63680490 Msa0217110:three_prime_UTR 25.0%
!!! TTTTAGGGGTAATTATGTTT+GGG + chr2_1:63680472-63680491 Msa0217110:three_prime_UTR 25.0%
! GTCTTATCAATGATAATTTC+AGG + chr2_1:63680494-63680513 Msa0217110:three_prime_UTR 25.0%
! CAAGATATGGTTTCTTTTTG+TGG - chr2_1:63677571-63677590 None:intergenic 30.0%
!! GGACTTGTATTTTCAAGATA+TGG - chr2_1:63677584-63677603 None:intergenic 30.0%
! CTTTTGCATTGGAAATTGTA+CGG - chr2_1:63677627-63677646 None:intergenic 30.0%
ATGCTGAAACAATACTCTTA+GGG + chr2_1:63677660-63677679 Msa0217110:intron 30.0%
!! TCTCTGAAGTTGTAATATAG+TGG + chr2_1:63678070-63678089 Msa0217110:intron 30.0%
ACATAATTAATGCTCTCCTT+CGG - chr2_1:63678112-63678131 None:intergenic 30.0%
!! AAGGAGAGCATTAATTATGT+AGG + chr2_1:63678112-63678131 Msa0217110:intron 30.0%
TCTTAAGTGTTACACTTTGT+GGG + chr2_1:63678824-63678843 Msa0217110:intron 30.0%
! AGTCATGAAAATGACTTTTC+TGG - chr2_1:63679005-63679024 None:intergenic 30.0%
!!! TGCTTATATTTTGAAACGGA+AGG - chr2_1:63679144-63679163 None:intergenic 30.0%
TTACTTTCTCTCTATTGCTT+TGG + chr2_1:63679446-63679465 Msa0217110:intron 30.0%
! GGTGTTACATAAATTGTCAA+CGG + chr2_1:63679506-63679525 Msa0217110:intron 30.0%
TATGAACATGAACTTTATCC+AGG + chr2_1:63679638-63679657 Msa0217110:CDS 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_1:63679702-63679721 Msa0217110:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_1:63679707-63679726 None:intergenic 30.0%
AAAATCCTTGGGAACATTAA+TGG + chr2_1:63679820-63679839 Msa0217110:CDS 30.0%
AAATCCTTGGGAACATTAAT+GGG + chr2_1:63679821-63679840 Msa0217110:CDS 30.0%
! AAATGATGGAACAAAAACTC+AGG + chr2_1:63680042-63680061 Msa0217110:CDS 30.0%
! ATGTCATTTCCTTGGAAATA+AGG + chr2_1:63680153-63680172 Msa0217110:CDS 30.0%
!! ATAAAAGAGCCTTATTTCCA+AGG - chr2_1:63680165-63680184 None:intergenic 30.0%
! TTGGAAATAAGGCTCTTTTA+TGG + chr2_1:63680164-63680183 Msa0217110:CDS 30.0%
! TGGAAATAAGGCTCTTTTAT+GGG + chr2_1:63680165-63680184 Msa0217110:CDS 30.0%
! CTTTTATGGGTACCTAATTT+AGG + chr2_1:63680178-63680197 Msa0217110:CDS 30.0%
!!! TAGTTTTATGTACCCTAAAG+AGG + chr2_1:63680353-63680372 Msa0217110:three_prime_UTR 30.0%
!!! TCGTGAGTAAATGTTTTTAG+GGG + chr2_1:63680458-63680477 Msa0217110:three_prime_UTR 30.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_1:63677330-63677349 Msa0217110:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_1:63677341-63677360 Msa0217110:CDS 35.0%
! AGAAGTTCTAACAGAGAAGT+GGG - chr2_1:63677382-63677401 None:intergenic 35.0%
! TAGAAGTTCTAACAGAGAAG+TGG - chr2_1:63677383-63677402 None:intergenic 35.0%
TTCCTTAAACAATGCCCTTA+GGG - chr2_1:63677547-63677566 None:intergenic 35.0%
GAAAATACAAGTCCAACAGA+TGG + chr2_1:63677590-63677609 Msa0217110:intron 35.0%
GATGCTGAAACAATACTCTT+AGG + chr2_1:63677659-63677678 Msa0217110:intron 35.0%
TTAGTAATCCTTTATCCCGT+AGG + chr2_1:63677798-63677817 Msa0217110:intron 35.0%
!! TATTGTTGAGTTGAATCCGA+AGG + chr2_1:63678093-63678112 Msa0217110:intron 35.0%
AGGTTGACAAATTTGAATGC+TGG - chr2_1:63678142-63678161 None:intergenic 35.0%
!! TTGTTAGGATGAAAGGTGTT+TGG - chr2_1:63678276-63678295 None:intergenic 35.0%
!! CATGTTAATTTGTGCCCTTA+GGG - chr2_1:63678463-63678482 None:intergenic 35.0%
!! TCATGTTAATTTGTGCCCTT+AGG - chr2_1:63678464-63678483 None:intergenic 35.0%
! ATCTTTTATGGGAGCTTCTA+TGG + chr2_1:63678606-63678625 Msa0217110:intron 35.0%
!!! AGTCAGGAAACTGACTTTTT+TGG + chr2_1:63678632-63678651 Msa0217110:intron 35.0%
AGTAATGCTTGCAAACATCT+TGG + chr2_1:63678733-63678752 Msa0217110:intron 35.0%
GTCTTAAGTGTTACACTTTG+TGG + chr2_1:63678823-63678842 Msa0217110:intron 35.0%
CTTAAGTGTTACACTTTGTG+GGG + chr2_1:63678825-63678844 Msa0217110:intron 35.0%
TCATGTGGGTCTCATAAATT+TGG - chr2_1:63679077-63679096 None:intergenic 35.0%
TGATGTATGTGGTCCAAATA+TGG + chr2_1:63679193-63679212 Msa0217110:intron 35.0%
TGATGTATGTGGTCCATATT+TGG - chr2_1:63679209-63679228 None:intergenic 35.0%
TGTGTGTCATCAATTTCCAT+GGG - chr2_1:63679599-63679618 None:intergenic 35.0%
TTGTGTGTCATCAATTTCCA+TGG - chr2_1:63679600-63679619 None:intergenic 35.0%
AAATCCCATTAATGTTCCCA+AGG - chr2_1:63679828-63679847 None:intergenic 35.0%
GGATTTGCAATTTCTAAGCT+TGG + chr2_1:63679842-63679861 Msa0217110:CDS 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_1:63679920-63679939 Msa0217110:CDS 35.0%
AGAAAGCAGTTATGTGTCAT+AGG + chr2_1:63679945-63679964 Msa0217110:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_1:63680006-63680025 Msa0217110:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_1:63680015-63680034 Msa0217110:CDS 35.0%
! AAACCTGTATGTCATTTCCT+TGG + chr2_1:63680145-63680164 Msa0217110:CDS 35.0%
TTTCCAAGGAAATGACATAC+AGG - chr2_1:63680151-63680170 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_1:63680183-63680202 Msa0217110:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_1:63680193-63680212 None:intergenic 35.0%
ATTTATGTTACACAGTCCCA+TGG - chr2_1:63680268-63680287 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_1:63680322-63680341 Msa0217110:three_prime_UTR 35.0%
ACAAAGATTGTGCCTCTTTA+GGG - chr2_1:63680368-63680387 None:intergenic 35.0%
AACAAAGATTGTGCCTCTTT+AGG - chr2_1:63680369-63680388 None:intergenic 35.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_1:63677329-63677348 Msa0217110:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_1:63677337-63677356 None:intergenic 40.0%
TCTAACAGAGAAGTGGGAAT+GGG - chr2_1:63677376-63677395 None:intergenic 40.0%
TTCTAACAGAGAAGTGGGAA+TGG - chr2_1:63677377-63677396 None:intergenic 40.0%
!! CTGTTAGAACTTCTAAAGCC+TGG + chr2_1:63677388-63677407 Msa0217110:CDS 40.0%
AAATAACAACCTTGAGGTCC+AGG - chr2_1:63677409-63677428 None:intergenic 40.0%
GTTCCTTAAACAATGCCCTT+AGG - chr2_1:63677548-63677567 None:intergenic 40.0%
TACAAGTCCAACAGATGGTA+AGG + chr2_1:63677595-63677614 Msa0217110:intron 40.0%
GTTATGACCTTACCATCTGT+TGG - chr2_1:63677605-63677624 None:intergenic 40.0%
AGATTCACACACATTACGTG+TGG - chr2_1:63677897-63677916 None:intergenic 40.0%
TGTGTGTGAATCTAGTCACT+AGG + chr2_1:63677904-63677923 Msa0217110:intron 40.0%
TGTGGGTCTCATAAATTTGG+AGG - chr2_1:63679074-63679093 None:intergenic 40.0%
TGAGTCTCGCTAAATCATGT+GGG - chr2_1:63679091-63679110 None:intergenic 40.0%
ATGAGTCTCGCTAAATCATG+TGG - chr2_1:63679092-63679111 None:intergenic 40.0%
AAACCCATCTATTATGGCAC+AGG + chr2_1:63679554-63679573 Msa0217110:intron 40.0%
ACACCTGTGCCATAATAGAT+GGG - chr2_1:63679560-63679579 None:intergenic 40.0%
AACACCTGTGCCATAATAGA+TGG - chr2_1:63679561-63679580 None:intergenic 40.0%
TGAAATTAATGACCTCCCCA+TGG + chr2_1:63679580-63679599 Msa0217110:CDS 40.0%
GTGTGTCATCAATTTCCATG+GGG - chr2_1:63679598-63679617 None:intergenic 40.0%
!! TGCACTTTCATTGTTTTGCC+TGG - chr2_1:63679659-63679678 None:intergenic 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_1:63679695-63679714 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_1:63679696-63679715 None:intergenic 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_1:63680018-63680037 Msa0217110:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_1:63680073-63680092 Msa0217110:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_1:63680118-63680137 Msa0217110:CDS 40.0%
TGACATACAGGTTTACTACC+AGG - chr2_1:63680139-63680158 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_1:63680196-63680215 Msa0217110:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_1:63680224-63680243 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_1:63680233-63680252 Msa0217110:exon 40.0%
TCTTGCTAACAAGTGCCCTA+AGG + chr2_1:63677529-63677548 Msa0217110:intron 45.0%
CTTGCTAACAAGTGCCCTAA+GGG + chr2_1:63677530-63677549 Msa0217110:intron 45.0%
!! TGCCCTAAGGGCATTGTTTA+AGG + chr2_1:63677542-63677561 Msa0217110:intron 45.0%
TACCAACTGAGCTACACCTA+CGG - chr2_1:63677817-63677836 None:intergenic 45.0%
! TACCCGGGATAATTTGTGCA+GGG + chr2_1:63677839-63677858 Msa0217110:intron 45.0%
ACATTACGTGTGGAGAAGTG+GGG - chr2_1:63677887-63677906 None:intergenic 45.0%
CACATTACGTGTGGAGAAGT+GGG - chr2_1:63677888-63677907 None:intergenic 45.0%
ACACATTACGTGTGGAGAAG+TGG - chr2_1:63677889-63677908 None:intergenic 45.0%
TACAGCACCAGACTGTACAT+CGG + chr2_1:63678242-63678261 Msa0217110:intron 45.0%
!! CTCCTTAACTTGTGCCCTAA+GGG + chr2_1:63678446-63678465 Msa0217110:intron 45.0%
TGATGCCACTGTACAAACAG+TGG + chr2_1:63678937-63678956 Msa0217110:intron 45.0%
GATGCCACTGTACAAACAGT+GGG + chr2_1:63678938-63678957 Msa0217110:intron 45.0%
ACATCCCACTGTTTGTACAG+TGG - chr2_1:63678945-63678964 None:intergenic 45.0%
TGTCATCAATTTCCATGGGG+AGG - chr2_1:63679595-63679614 None:intergenic 45.0%
GTAAAGAAAGCAGCTGCAGA+AGG + chr2_1:63679779-63679798 Msa0217110:CDS 45.0%
! GTTCCATTGGTGGCAAATGA+TGG + chr2_1:63680028-63680047 Msa0217110:CDS 45.0%
GTTCCATCATTTGCCACCAA+TGG - chr2_1:63680034-63680053 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_1:63680215-63680234 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_1:63680216-63680235 Msa0217110:CDS 45.0%
ATCATGGTTGGACACACCAT+GGG + chr2_1:63680249-63680268 Msa0217110:three_prime_UTR 45.0%
CTTCTTCAGGCAGTGATGCA+TGG - chr2_1:63677324-63677343 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_1:63677367-63677386 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_1:63677368-63677387 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCTCA+AGG + chr2_1:63677397-63677416 Msa0217110:CDS 50.0%
GAGCTACACCTACGGGATAA+AGG - chr2_1:63677809-63677828 None:intergenic 50.0%
ACCAACTGAGCTACACCTAC+GGG - chr2_1:63677816-63677835 None:intergenic 50.0%
!! TAGCTCAGTTGGTAGCTACC+CGG + chr2_1:63677823-63677842 Msa0217110:intron 50.0%
! CTACCCGGGATAATTTGTGC+AGG + chr2_1:63677838-63677857 Msa0217110:intron 50.0%
! ACCCGGGATAATTTGTGCAG+GGG + chr2_1:63677840-63677859 Msa0217110:intron 50.0%
TCAACCTCTGCTACAGCTGT+AGG + chr2_1:63678155-63678174 Msa0217110:intron 50.0%
ACCAGACTGTACATCGGTAG+AGG + chr2_1:63678248-63678267 Msa0217110:intron 50.0%
TCCTCTACCGATGTACAGTC+TGG - chr2_1:63678252-63678271 None:intergenic 50.0%
!! GCTCCTTAACTTGTGCCCTA+AGG + chr2_1:63678445-63678464 Msa0217110:intron 50.0%
TGCCCTTAGGGCACAAGTTA+AGG - chr2_1:63678451-63678470 None:intergenic 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_1:63679689-63679708 Msa0217110:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_1:63680237-63680256 Msa0217110:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_1:63680250-63680269 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_1:63680248-63680267 Msa0217110:three_prime_UTR 50.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_1:63677292-63677311 Msa0217110:intron 55.0%
TCCCGTAGGTGTAGCTCAGT+TGG + chr2_1:63677812-63677831 Msa0217110:intron 55.0%
!! AGCTCAGTTGGTAGCTACCC+GGG + chr2_1:63677824-63677843 Msa0217110:intron 55.0%
GCCCCTGCACAAATTATCCC+GGG - chr2_1:63677844-63677863 None:intergenic 55.0%
GGCCCCTGCACAAATTATCC+CGG - chr2_1:63677845-63677864 None:intergenic 55.0%
! GGGATAATTTGTGCAGGGGC+CGG + chr2_1:63677844-63677863 Msa0217110:intron 55.0%
! GGATAATTTGTGCAGGGGCC+GGG + chr2_1:63677845-63677864 Msa0217110:intron 55.0%
! GATAATTTGTGCAGGGGCCG+GGG + chr2_1:63677846-63677865 Msa0217110:intron 55.0%
GTGGAGAAGTGGGGAATCCA+GGG - chr2_1:63677878-63677897 None:intergenic 55.0%
TGTGGAGAAGTGGGGAATCC+AGG - chr2_1:63677879-63677898 None:intergenic 55.0%
TGGTCCTACAGCTGTAGCAG+AGG - chr2_1:63678162-63678181 None:intergenic 55.0%
GGAGCTTCTATGGTGCAGTC+AGG + chr2_1:63678616-63678635 Msa0217110:intron 55.0%
GTGCAGCTTGCACTTGCAGG+AGG + chr2_1:63677295-63677314 Msa0217110:intron 60.0%
GGAATCCAGGGTTCGAACCC+CGG - chr2_1:63677866-63677885 None:intergenic 60.0%
AGGGGCCGGGGTTCGAACCC+TGG + chr2_1:63677858-63677877 Msa0217110:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 63677134 63680535 63677134 ID=Msa0217110;Name=Msa0217110
chr2_1 mRNA 63677134 63680535 63677134 ID=Msa0217110-mRNA-1;Parent=Msa0217110;Name=Msa0217110-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0.5|0.66|0.66|1|1|3|298|261
chr2_1 exon 63677134 63677139 63677134 ID=Msa0217110-mRNA-1:exon:17660;Parent=Msa0217110-mRNA-1
chr2_1 exon 63677301 63677418 63677301 ID=Msa0217110-mRNA-1:exon:17661;Parent=Msa0217110-mRNA-1
chr2_1 exon 63679576 63680535 63679576 ID=Msa0217110-mRNA-1:exon:17662;Parent=Msa0217110-mRNA-1
chr2_1 CDS 63677134 63677139 63677134 ID=Msa0217110-mRNA-1:cds;Parent=Msa0217110-mRNA-1
chr2_1 CDS 63677301 63677418 63677301 ID=Msa0217110-mRNA-1:cds;Parent=Msa0217110-mRNA-1
chr2_1 CDS 63679576 63680237 63679576 ID=Msa0217110-mRNA-1:cds;Parent=Msa0217110-mRNA-1
chr2_1 three_prime_UTR 63680238 63680535 63680238 ID=Msa0217110-mRNA-1:three_prime_utr;Parent=Msa0217110-mRNA-1
Gene Sequence

>Msa0217110

ATGACGCTTGCACTTGCAGGAGGAAGCCATGCATCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTCTGTTAGAACTTCTAAAGCCTGGACCTCAAGGTGTTGAAATTAATGACCTCCCCATGGAAATTGATGACACACAATACCCAAAAACATTTTTCTATGAACATGAACTTTATCCAGGCAAAACAATGAAAGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAAGTATGTGTAAAGAAAGCAGCTGCAGAAGGAGAACAAAAATTTTGTGCAAAATCCTTGGGAACATTAATGGGATTTGCAATTTCTAAGCTTGGAAAAAATATTCAAGCACTTTCAAGCTCCTTCATAGATAAACATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTTATGTGTCATAGGTTGAATTTTCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCAAATGATGGAACAAAAACTCAGGCACTCGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGCTGAAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTATGTCATTTCCTTGGAAATAAGGCTCTTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa0217110

MTLALAGGSHASLPEEEYWEAVWPNTPIPTSLLELLKPGPQGVEINDLPMEIDDTQYPKTFFYEHELYPGKTMKVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLMGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLKQIMKADPGSKPVCHFLGNKALLWVPNLGLDNAYGANVAV*