AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0256730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256730 MtrunA17_Chr2g0318901 94.505 273 15 0 1 273 1 273 0.0 546
Msa0256730 MtrunA17_Chr2g0318871 83.150 273 46 0 1 273 1 273 6.66e-176 485
Msa0256730 MtrunA17_Chr2g0318931 63.569 269 89 5 1 261 5 272 2.61e-123 352
Msa0256730 MtrunA17_Chr8g0355861 44.776 268 133 7 1 260 1 261 9.12e-78 237
Msa0256730 MtrunA17_Chr8g0355321 37.217 309 130 6 10 260 1 303 3.60e-73 226
Msa0256730 MtrunA17_Chr1g0168781 42.435 271 142 6 1 264 1 264 3.52e-72 222
Msa0256730 MtrunA17_Chr8g0355871 45.918 196 97 3 71 259 29 222 2.35e-60 191
Msa0256730 MtrunA17_Chr8g0355311 48.824 170 80 2 96 265 34 196 1.76e-57 182
Msa0256730 MtrunA17_Chr8g0355841 53.165 158 73 1 111 268 362 518 2.50e-57 191
Msa0256730 MtrunA17_Chr8g0355331 45.294 170 86 2 97 266 266 428 3.43e-53 178
Msa0256730 MtrunA17_Chr8g0355831 54.667 150 67 1 111 260 204 352 2.21e-51 172
Msa0256730 MtrunA17_Chr8g0355831 29.752 121 66 3 28 133 26 142 9.96e-11 61.6
Msa0256730 MtrunA17_Chr1g0147261 33.663 303 149 12 1 257 1 297 3.40e-44 151
Msa0256730 MtrunA17_Chr3g0139431 31.579 323 146 12 6 259 5 321 3.42e-44 152
Msa0256730 MtrunA17_Chr1g0147311 28.746 327 158 9 5 258 30 354 3.44e-39 140
Msa0256730 MtrunA17_Chr1g0147331 29.538 325 153 11 5 258 4 323 5.43e-38 136
Msa0256730 MtrunA17_Chr1g0147241 30.671 313 158 12 1 257 1 310 1.89e-37 134
Msa0256730 MtrunA17_Chr1g0146631 28.923 325 155 11 5 258 4 323 1.44e-36 132
Msa0256730 MtrunA17_Chr1g0147291 32.456 228 126 9 57 258 40 265 9.27e-31 115
Msa0256730 MtrunA17_Chr1g0147301 32.618 233 123 10 57 258 54 283 1.35e-30 115
Msa0256730 MtrunA17_Chr1g0147271 34.579 214 111 7 73 258 171 383 5.02e-30 116
Msa0256730 MtrunA17_Chr1g0147321 32.456 228 126 9 56 257 10 235 7.10e-30 113
Msa0256730 MtrunA17_Chr1g0147251 31.278 227 123 6 58 257 20 240 1.21e-29 112
Msa0256730 MtrunA17_Chr3g0144421 29.310 232 129 10 59 259 75 302 1.31e-19 86.7
Msa0256730 MtrunA17_Chr4g0034821 29.778 225 116 11 73 261 75 293 4.27e-18 82.0
Msa0256730 MtrunA17_Chr3g0138231 31.250 144 89 4 128 261 125 268 1.96e-15 74.3
Msa0256730 MtrunA17_Chr4g0034731 33.566 143 83 5 129 259 290 432 6.90e-15 73.9
Msa0256730 MtrunA17_Chr4g0034721 34.965 143 81 6 129 259 434 576 1.31e-14 73.6
Msa0256730 MtrunA17_Chr3g0144451 32.639 144 87 5 128 261 134 277 1.55e-14 72.0
Msa0256730 MtrunA17_Chr4g0076231 26.897 145 95 5 123 257 13 156 5.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0256730 AT5G25610.1 33.036 224 121 8 63 257 166 389 7.52e-29 113
Msa0256730 AT1G49320.1 34.459 148 82 5 126 258 131 278 2.78e-16 77.4

Find 62 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCATTTCCTTGGAAATA+AGG 0.188317 2_2:+51723340 Msa0256730:CDS
ACAAAGATTGTGCCTCTTTA+GGG 0.230864 2_2:-51723552 None:intergenic
AACAAAGATTGTGCCTCTTT+AGG 0.250870 2_2:-51723553 None:intergenic
GTCTTATCAATGATAATTTC+AGG 0.264308 2_2:+51723681 Msa0256730:three_prime_UTR
GACAATAAAATACAACCTTT+TGG 0.266567 2_2:-51723163 None:intergenic
CGTCCCTTTGCACAACCTTA+TGG 0.298036 2_2:+51722876 Msa0256730:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.300262 2_2:+51721681 Msa0256730:CDS
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 2_2:+51723403 Msa0256730:CDS
GCTTCCCAATATTCTTCTTC+AGG 0.355894 2_2:-51721686 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 2_2:+51721693 Msa0256730:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 2_2:-51723377 None:intergenic
AAATCCTTAGGAACATTAAT+CGG 0.372556 2_2:+51723008 Msa0256730:CDS
GTTTGTCATGCTGATACTTC+AGG 0.375794 2_2:+51723260 Msa0256730:CDS
GGATTTGCAATTTCTAAGCT+TGG 0.386794 2_2:+51723029 Msa0256730:CDS
TATGAACATGAACTTTATCC+TGG 0.401999 2_2:+51722825 Msa0256730:CDS
AAACCTGTTTGTCATTTCCT+TGG 0.406653 2_2:+51723332 Msa0256730:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.411503 2_2:+51721644 Msa0256730:intron
ACTGCCTGAAGAAGAATATT+GGG 0.412787 2_2:+51721682 Msa0256730:CDS
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 2_2:+51723107 Msa0256730:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 2_2:+51723370 Msa0256730:CDS
TTGTGTGTCATCAATTTCCA+TGG 0.427694 2_2:-51722784 None:intergenic
AGAAAGCAGTTATGTGTCAT+AGG 0.437124 2_2:+51723132 Msa0256730:CDS
TTGTTCATGCTTATCTATGA+AGG 0.440092 2_2:-51723078 None:intergenic
AAATAACAACCTTGAGGTCC+AGG 0.452077 2_2:-51721758 None:intergenic
TAGAAGTTCTCGAAGAGAAG+TGG 0.456535 2_2:-51721732 None:intergenic
TTTCCAAGGAAATGACAAAC+AGG 0.462441 2_2:-51723335 None:intergenic
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 2_2:+51723509 Msa0256730:three_prime_UTR
AACCTTATGGTGTTTATACA+TGG 0.471062 2_2:+51722889 Msa0256730:CDS
CTTCTTCAGGCAGTGATGCA+TGG 0.473820 2_2:-51721673 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.474967 2_2:-51721716 None:intergenic
ACACCATAAGGTTGTGCAAA+GGG 0.481077 2_2:-51722879 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 2_2:+51723383 Msa0256730:CDS
TTCTCGAAGAGAAGTGGGAA+TGG 0.495855 2_2:-51721726 None:intergenic
CTTCGAGAACTTCTAAAGCC+TGG 0.497324 2_2:+51721740 Msa0256730:CDS
GGAGTACTCTCCATCATGGT+TGG 0.497653 2_2:+51723424 Msa0256730:exon
ATCCATGTATAAACACCATA+AGG 0.501910 2_2:-51722891 None:intergenic
AGAAGTGGGAATGGGAGTGT+TGG 0.505103 2_2:-51721717 None:intergenic
GTTCCATCATTAGCCACCAA+TGG 0.510862 2_2:-51723218 None:intergenic
TCATAGGTTGAATTTCCAAA+AGG 0.512621 2_2:+51723148 Msa0256730:CDS
CAGATTATGAAAGCTGATCC+TGG 0.522799 2_2:+51723305 Msa0256730:CDS
ATAAAAGAGCCTTATTTCCA+AGG 0.523232 2_2:-51723349 None:intergenic
AAATCCGATTAATGTTCCTA+AGG 0.524830 2_2:-51723012 None:intergenic
GTTCCATTGGTGGCTAATGA+TGG 0.535070 2_2:+51723215 Msa0256730:CDS
TGTGTGTCATCAATTTCCAT+GGG 0.537514 2_2:-51722783 None:intergenic
TCTCGAAGAGAAGTGGGAAT+GGG 0.548515 2_2:-51721725 None:intergenic
TTTAGGAGTACTCTCCATCA+TGG 0.550450 2_2:+51723420 Msa0256730:exon
CTTCTAAAGCCTGGACCTCA+AGG 0.560362 2_2:+51721749 Msa0256730:CDS
AACAGCAACATTGGCACCAT+AGG 0.567015 2_2:-51723399 None:intergenic
AGAAGTTCTCGAAGAGAAGT+GGG 0.572137 2_2:-51721731 None:intergenic
GTAAAGAAAGCAGCTGCAGA+AGG 0.576977 2_2:+51722966 Msa0256730:CDS
ATTTATGTTACACAGTCCCA+TGG 0.579361 2_2:-51723452 None:intergenic
ATCATGGTTGGACACACCAT+GGG 0.579868 2_2:+51723436 Msa0256730:three_prime_UTR
GTACTCCTAAACAGCAACAT+TGG 0.581094 2_2:-51723408 None:intergenic
AGAAGGATACACATTTAATG+AGG 0.587845 2_2:+51722938 Msa0256730:CDS
CATCATGGTTGGACACACCA+TGG 0.594647 2_2:+51723435 Msa0256730:three_prime_UTR
TGTCATCAATTTCCATGGGG+TGG 0.605445 2_2:-51722779 None:intergenic
GAACTGAAATGAAAACGAGA+AGG 0.613747 2_2:-51721264 None:intergenic
TGACAAACAGGTTTACTACC+AGG 0.616609 2_2:-51723323 None:intergenic
CATGGTGTGTCCAACCATGA+TGG 0.616911 2_2:-51723434 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 2_2:-51722880 None:intergenic
TGAAATTGATGACCACCCCA+TGG 0.663021 2_2:+51722767 Msa0256730:CDS
GTGTGTCATCAATTTCCATG+GGG 0.716189 2_2:-51722782 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCACAATATTAATTAATAT+AGG - chr2_2:51721860-51721879 None:intergenic 10.0%
!!! TAATCCTTTATTAATTTTAT+TGG + chr2_2:51721965-51721984 Msa0256730:intron 10.0%
!!! CATTTAAAATATAAAAAAGT+TGG + chr2_2:51722218-51722237 Msa0256730:intron 10.0%
!! ACATTATTAATTTATTTGTT+AGG - chr2_2:51722459-51722478 None:intergenic 10.0%
!!! ATGTTTTAATATAATGAAAA+TGG + chr2_2:51723593-51723612 Msa0256730:three_prime_UTR 10.0%
!! TGATCCAATAAAATTAATAA+AGG - chr2_2:51721972-51721991 None:intergenic 15.0%
!! ATCGATATTTACTTTATATT+TGG + chr2_2:51722183-51722202 Msa0256730:intron 15.0%
!!! TATTTTAAATGTATTAGGTT+GGG - chr2_2:51722209-51722228 None:intergenic 15.0%
!!! ATATTTTAAATGTATTAGGT+TGG - chr2_2:51722210-51722229 None:intergenic 15.0%
!! TATTTGTCATATAAATTGAA+TGG - chr2_2:51722350-51722369 None:intergenic 15.0%
!! TTTATTAGTTAAAATTCAGA+TGG + chr2_2:51722587-51722606 Msa0256730:intron 15.0%
!! TTATTAGTTAAAATTCAGAT+GGG + chr2_2:51722588-51722607 Msa0256730:intron 15.0%
!!! TAACACATATTCTTTTTTAT+TGG - chr2_2:51722667-51722686 None:intergenic 15.0%
!! AAAGAATATGTGTTAAATTA+TGG + chr2_2:51722672-51722691 Msa0256730:intron 15.0%
!! AAAAAAAAATATCTACAATG+AGG - chr2_2:51723626-51723645 None:intergenic 15.0%
!! TTAAACAACAACCTAAAATA+TGG + chr2_2:51721386-51721405 Msa0256730:intron 20.0%
!!! TACTTTAATTGCCATATTTT+AGG - chr2_2:51721400-51721419 None:intergenic 20.0%
!! ATTATTACTTACATATGTTG+AGG + chr2_2:51721436-51721455 Msa0256730:intron 20.0%
!! TTATTACTTACATATGTTGA+GGG + chr2_2:51721437-51721456 Msa0256730:intron 20.0%
!! AAAATTAAATAACAACCTTG+AGG - chr2_2:51721767-51721786 None:intergenic 20.0%
!!! TCAGAAAAGGTAAATTTTAT+GGG + chr2_2:51721907-51721926 Msa0256730:intron 20.0%
!! TTATATGACAAATAATTCCA+AGG + chr2_2:51722356-51722375 Msa0256730:intron 20.0%
!! TATATGACAAATAATTCCAA+GGG + chr2_2:51722357-51722376 Msa0256730:intron 20.0%
!!! AATTTATTTGTTAGGATGAA+AGG - chr2_2:51722451-51722470 None:intergenic 20.0%
!! TCTCTCAAGTTATATATTAT+TGG - chr2_2:51722485-51722504 None:intergenic 20.0%
!!! TATTTTAAACCCATCTATTA+TGG + chr2_2:51722735-51722754 Msa0256730:intron 20.0%
!!! GTTCATAGAAAAATGTTTTT+GGG - chr2_2:51722812-51722831 None:intergenic 20.0%
!!! TGTTCATAGAAAAATGTTTT+TGG - chr2_2:51722813-51722832 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + chr2_2:51722921-51722940 Msa0256730:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + chr2_2:51723176-51723195 Msa0256730:CDS 20.0%
! TAAGTTTATAGTACATCAGT+TGG - chr2_2:51721369-51721388 None:intergenic 25.0%
! GTAAGAAATAAACTCAGAAA+AGG + chr2_2:51721894-51721913 Msa0256730:intron 25.0%
!! CTCAGAAAAGGTAAATTTTA+TGG + chr2_2:51721906-51721925 Msa0256730:intron 25.0%
! AGAGATGAAAAACTAATAAG+GGG - chr2_2:51722023-51722042 None:intergenic 25.0%
! CAGAGATGAAAAACTAATAA+GGG - chr2_2:51722024-51722043 None:intergenic 25.0%
! TCAGAGATGAAAAACTAATA+AGG - chr2_2:51722025-51722044 None:intergenic 25.0%
! ACAAAATTAATACTCTCCTT+CGG - chr2_2:51722075-51722094 None:intergenic 25.0%
!!! AAGGAGAGTATTAATTTTGT+AGG + chr2_2:51722075-51722094 Msa0256730:intron 25.0%
!! AAATTTTGTGCAAAATCCTT+AGG + chr2_2:51722996-51723015 Msa0256730:CDS 25.0%
! AAATCCTTAGGAACATTAAT+CGG + chr2_2:51723008-51723027 Msa0256730:CDS 25.0%
!! GACAATAAAATACAACCTTT+TGG - chr2_2:51723166-51723185 None:intergenic 25.0%
!! TTTCGTGAGTAAATGTTTTT+AGG + chr2_2:51723643-51723662 Msa0256730:three_prime_UTR 25.0%
!!! TTCGTGAGTAAATGTTTTTA+GGG + chr2_2:51723644-51723663 Msa0256730:three_prime_UTR 25.0%
!!! TTTTTAGGGGTAATTATGTT+TGG + chr2_2:51723658-51723677 Msa0256730:three_prime_UTR 25.0%
!!! TTTTAGGGGTAATTATGTTT+GGG + chr2_2:51723659-51723678 Msa0256730:three_prime_UTR 25.0%
! GTCTTATCAATGATAATTTC+AGG + chr2_2:51723681-51723700 Msa0256730:three_prime_UTR 25.0%
AAGAGTTCTTAAGTGTTTAG+AGG - chr2_2:51721334-51721353 None:intergenic 30.0%
TCTAAACACTTAAGAACTCT+TGG + chr2_2:51721333-51721352 Msa0256730:intron 30.0%
AGGTTGACAAATTTGAATGT+TGG - chr2_2:51722105-51722124 None:intergenic 30.0%
! TGACTCTATAATTTTTGCCT+AGG + chr2_2:51722281-51722300 Msa0256730:intron 30.0%
TAAATTTAGAAAGAGACCCT+TGG - chr2_2:51722376-51722395 None:intergenic 30.0%
! GAGAGATGTTATGATTTTTC+TGG + chr2_2:51722501-51722520 Msa0256730:intron 30.0%
TCATGTGGGTTTCATAAATT+TGG - chr2_2:51722618-51722637 None:intergenic 30.0%
TTTATGAAACCCACATGATT+TGG + chr2_2:51722620-51722639 Msa0256730:intron 30.0%
TATGAACATGAACTTTATCC+TGG + chr2_2:51722825-51722844 Msa0256730:CDS 30.0%
AACCTTATGGTGTTTATACA+TGG + chr2_2:51722889-51722908 Msa0256730:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - chr2_2:51722894-51722913 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + chr2_2:51722938-51722957 Msa0256730:CDS 30.0%
AAATCCGATTAATGTTCCTA+AGG - chr2_2:51723015-51723034 None:intergenic 30.0%
TTGTTCATGCTTATCTATGA+AGG - chr2_2:51723081-51723100 None:intergenic 30.0%
! TCATAGGTTGAATTTCCAAA+AGG + chr2_2:51723148-51723167 Msa0256730:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + chr2_2:51723340-51723359 Msa0256730:CDS 30.0%
!! ATAAAAGAGCCTTATTTCCA+AGG - chr2_2:51723352-51723371 None:intergenic 30.0%
! TTGGAAATAAGGCTCTTTTA+TGG + chr2_2:51723351-51723370 Msa0256730:CDS 30.0%
! TGGAAATAAGGCTCTTTTAT+GGG + chr2_2:51723352-51723371 Msa0256730:CDS 30.0%
! CTTTTATGGGTACCTAATTT+AGG + chr2_2:51723365-51723384 Msa0256730:CDS 30.0%
!!! TAGTTTTATGTACCCTAAAG+AGG + chr2_2:51723540-51723559 Msa0256730:three_prime_UTR 30.0%
!!! TCGTGAGTAAATGTTTTTAG+GGG + chr2_2:51723645-51723664 Msa0256730:three_prime_UTR 30.0%
GAACTGAAATGAAAACGAGA+AGG - chr2_2:51721267-51721286 None:intergenic 35.0%
!!! GAAATGAAAGGTTTTAGAGC+AGG - chr2_2:51721305-51721324 None:intergenic 35.0%
!! TAGAGGATGATGGAAATGAA+AGG - chr2_2:51721317-51721336 None:intergenic 35.0%
CTTAAGTGTTTAGAGGATGA+TGG - chr2_2:51721327-51721346 None:intergenic 35.0%
ACTGCCTGAAGAAGAATATT+GGG + chr2_2:51721682-51721701 Msa0256730:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + chr2_2:51721693-51721712 Msa0256730:CDS 35.0%
!! TCTCTGAAGCTGTAATATAG+TGG + chr2_2:51722038-51722057 Msa0256730:intron 35.0%
!! TTGTTAGGATGAAAGGTGTT+TGG - chr2_2:51722444-51722463 None:intergenic 35.0%
TGTGGGTTTCATAAATTTGG+AGG - chr2_2:51722615-51722634 None:intergenic 35.0%
!! TATGGTGTCACAGTCAAAAT+TGG + chr2_2:51722690-51722709 Msa0256730:intron 35.0%
TGTGTGTCATCAATTTCCAT+GGG - chr2_2:51722786-51722805 None:intergenic 35.0%
TTGTGTGTCATCAATTTCCA+TGG - chr2_2:51722787-51722806 None:intergenic 35.0%
GGATTTGCAATTTCTAAGCT+TGG + chr2_2:51723029-51723048 Msa0256730:CDS 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + chr2_2:51723107-51723126 Msa0256730:CDS 35.0%
AGAAAGCAGTTATGTGTCAT+AGG + chr2_2:51723132-51723151 Msa0256730:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + chr2_2:51723193-51723212 Msa0256730:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + chr2_2:51723202-51723221 Msa0256730:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + chr2_2:51723332-51723351 Msa0256730:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - chr2_2:51723338-51723357 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + chr2_2:51723370-51723389 Msa0256730:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - chr2_2:51723380-51723399 None:intergenic 35.0%
ATTTATGTTACACAGTCCCA+TGG - chr2_2:51723455-51723474 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + chr2_2:51723509-51723528 Msa0256730:three_prime_UTR 35.0%
ACAAAGATTGTGCCTCTTTA+GGG - chr2_2:51723555-51723574 None:intergenic 35.0%
AACAAAGATTGTGCCTCTTT+AGG - chr2_2:51723556-51723575 None:intergenic 35.0%
ACTTACATATGTTGAGGGTG+TGG + chr2_2:51721442-51721461 Msa0256730:intron 40.0%
! CTTACATATGTTGAGGGTGT+GGG + chr2_2:51721443-51721462 Msa0256730:intron 40.0%
CACTGCCTGAAGAAGAATAT+TGG + chr2_2:51721681-51721700 Msa0256730:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - chr2_2:51721689-51721708 None:intergenic 40.0%
AGAAGTTCTCGAAGAGAAGT+GGG - chr2_2:51721734-51721753 None:intergenic 40.0%
TAGAAGTTCTCGAAGAGAAG+TGG - chr2_2:51721735-51721754 None:intergenic 40.0%
AAATAACAACCTTGAGGTCC+AGG - chr2_2:51721761-51721780 None:intergenic 40.0%
AGTGGTATTGTTGAATCCGA+AGG + chr2_2:51722056-51722075 Msa0256730:intron 40.0%
! CCAGGAATTTTGTAAAGCCT+AGG - chr2_2:51722301-51722320 None:intergenic 40.0%
CCTAGGCTTTACAAAATTCC+TGG + chr2_2:51722298-51722317 Msa0256730:intron 40.0%
TACAGCACCATACTGTACAT+CGG + chr2_2:51722410-51722429 Msa0256730:intron 40.0%
TGAGTCTCACCAAATCATGT+GGG - chr2_2:51722632-51722651 None:intergenic 40.0%
ATGAGTCTCACCAAATCATG+TGG - chr2_2:51722633-51722652 None:intergenic 40.0%
AAACCCATCTATTATGGCAC+AGG + chr2_2:51722741-51722760 Msa0256730:intron 40.0%
ACACCTGTGCCATAATAGAT+GGG - chr2_2:51722747-51722766 None:intergenic 40.0%
AACACCTGTGCCATAATAGA+TGG - chr2_2:51722748-51722767 None:intergenic 40.0%
GTGTGTCATCAATTTCCATG+GGG - chr2_2:51722785-51722804 None:intergenic 40.0%
!! TGCACTTTCATTGTTTTGCC+AGG - chr2_2:51722846-51722865 None:intergenic 40.0%
ACACCATAAGGTTGTGCAAA+GGG - chr2_2:51722882-51722901 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - chr2_2:51722883-51722902 None:intergenic 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + chr2_2:51723205-51723224 Msa0256730:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + chr2_2:51723260-51723279 Msa0256730:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + chr2_2:51723305-51723324 Msa0256730:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - chr2_2:51723326-51723345 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + chr2_2:51723383-51723402 Msa0256730:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - chr2_2:51723411-51723430 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + chr2_2:51723420-51723439 Msa0256730:exon 40.0%
TCTCGAAGAGAAGTGGGAAT+GGG - chr2_2:51721728-51721747 None:intergenic 45.0%
TTCTCGAAGAGAAGTGGGAA+TGG - chr2_2:51721729-51721748 None:intergenic 45.0%
!! CTTCGAGAACTTCTAAAGCC+TGG + chr2_2:51721740-51721759 Msa0256730:CDS 45.0%
ACCATACTGTACATCGGTAG+AGG + chr2_2:51722416-51722435 Msa0256730:intron 45.0%
TCCTCTACCGATGTACAGTA+TGG - chr2_2:51722420-51722439 None:intergenic 45.0%
! TGAAATTGATGACCACCCCA+TGG + chr2_2:51722767-51722786 Msa0256730:CDS 45.0%
TGTCATCAATTTCCATGGGG+TGG - chr2_2:51722782-51722801 None:intergenic 45.0%
GTAAAGAAAGCAGCTGCAGA+AGG + chr2_2:51722966-51722985 Msa0256730:CDS 45.0%
! GTTCCATTGGTGGCTAATGA+TGG + chr2_2:51723215-51723234 Msa0256730:CDS 45.0%
GTTCCATCATTAGCCACCAA+TGG - chr2_2:51723221-51723240 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - chr2_2:51723402-51723421 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + chr2_2:51723403-51723422 Msa0256730:CDS 45.0%
ATCATGGTTGGACACACCAT+GGG + chr2_2:51723436-51723455 Msa0256730:three_prime_UTR 45.0%
!!! TTTTATATTTTAAATGTATT+AGG - chr2_2:51722214-51722233 None:intergenic 5.0%
!!! ATTTAAAATATAAAAAAGTT+GGG + chr2_2:51722219-51722238 Msa0256730:intron 5.0%
! ACATATGTTGAGGGTGTGGG+CGG + chr2_2:51721446-51721465 Msa0256730:intron 50.0%
CTTCTTCAGGCAGTGATGCA+TGG - chr2_2:51721676-51721695 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - chr2_2:51721719-51721738 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - chr2_2:51721720-51721739 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCTCA+AGG + chr2_2:51721749-51721768 Msa0256730:CDS 50.0%
AAAATTCCTGGCTCCACCAC+TGG + chr2_2:51722310-51722329 Msa0256730:intron 50.0%
CGTCCCTTTGCACAACCTTA+TGG + chr2_2:51722876-51722895 Msa0256730:CDS 50.0%
! GGAGTACTCTCCATCATGGT+TGG + chr2_2:51723424-51723443 Msa0256730:exon 50.0%
CATGGTGTGTCCAACCATGA+TGG - chr2_2:51723437-51723456 None:intergenic 50.0%
CATCATGGTTGGACACACCA+TGG + chr2_2:51723435-51723454 Msa0256730:three_prime_UTR 50.0%
GATGTGCAGCTTGCACTTGC+AGG + chr2_2:51721644-51721663 Msa0256730:intron 55.0%
TGTCAACCTCTGCTGCAGCC+AGG + chr2_2:51722116-51722135 Msa0256730:intron 60.0%
GTCAACCTCTGCTGCAGCCA+GGG + chr2_2:51722117-51722136 Msa0256730:intron 60.0%
TCAACCTCTGCTGCAGCCAG+GGG + chr2_2:51722118-51722137 Msa0256730:intron 60.0%
ATTGAGCCCAGACGAGTGCC+CGG - chr2_2:51722165-51722184 None:intergenic 60.0%
! TGTTGAGGGTGTGGGCGGTG+TGG + chr2_2:51721451-51721470 Msa0256730:intron 65.0%
TTGAGCCCAGACGAGTGCCC+GGG - chr2_2:51722164-51722183 None:intergenic 65.0%
CCTACAGCTGCAGCCAGTGG+TGG - chr2_2:51722326-51722345 None:intergenic 65.0%
! CCACCACTGGCTGCAGCTGT+AGG + chr2_2:51722323-51722342 Msa0256730:intron 65.0%
GGTCCTACAGCTGCAGCCAG+TGG - chr2_2:51722329-51722348 None:intergenic 65.0%
TTCGCCCCTGGCTGCAGCAG+AGG - chr2_2:51722125-51722144 None:intergenic 70.0%
TCTGCTGCAGCCAGGGGCGA+AGG + chr2_2:51722124-51722143 Msa0256730:intron 70.0%
! CTGCAGCCAGTGGTGGAGCC+AGG - chr2_2:51722319-51722338 None:intergenic 70.0%
!! CCAGGGGCGAAGGCACCACC+CGG + chr2_2:51722134-51722153 Msa0256730:intron 75.0%
! AAGGCACCACCCGGCGAGCC+CGG + chr2_2:51722143-51722162 Msa0256730:intron 75.0%
AGACGAGTGCCCGGGCTCGC+CGG - chr2_2:51722156-51722175 None:intergenic 75.0%
GCGAGCCCGGGCACTCGTCT+GGG + chr2_2:51722156-51722175 Msa0256730:intron 75.0%
! CCGGGTGGTGCCTTCGCCCC+TGG - chr2_2:51722137-51722156 None:intergenic 80.0%
! AGGCACCACCCGGCGAGCCC+GGG + chr2_2:51722144-51722163 Msa0256730:intron 80.0%
! GACGAGTGCCCGGGCTCGCC+GGG - chr2_2:51722155-51722174 None:intergenic 80.0%
! GGCGAGCCCGGGCACTCGTC+TGG + chr2_2:51722155-51722174 Msa0256730:intron 80.0%
! GAGTGCCCGGGCTCGCCGGG+TGG - chr2_2:51722152-51722171 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 51721250 51723722 51721250 ID=Msa0256730;Name=Msa0256730
chr2_2 mRNA 51721250 51723722 51721250 ID=Msa0256730-mRNA-1;Parent=Msa0256730;Name=Msa0256730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|1|0.66|1|1|1|3|298|273
chr2_2 exon 51721250 51721291 51721250 ID=Msa0256730-mRNA-1:exon:15580;Parent=Msa0256730-mRNA-1
chr2_2 exon 51721653 51721770 51721653 ID=Msa0256730-mRNA-1:exon:15581;Parent=Msa0256730-mRNA-1
chr2_2 exon 51722763 51723722 51722763 ID=Msa0256730-mRNA-1:exon:15582;Parent=Msa0256730-mRNA-1
chr2_2 CDS 51721250 51721291 51721250 ID=Msa0256730-mRNA-1:cds;Parent=Msa0256730-mRNA-1
chr2_2 CDS 51721653 51721770 51721653 ID=Msa0256730-mRNA-1:cds;Parent=Msa0256730-mRNA-1
chr2_2 CDS 51722763 51723424 51722763 ID=Msa0256730-mRNA-1:cds;Parent=Msa0256730-mRNA-1
chr2_2 three_prime_UTR 51723425 51723722 51723425 ID=Msa0256730-mRNA-1:three_prime_utr;Parent=Msa0256730-mRNA-1
Gene Sequence

>Msa0256730

ATGGGATTTCATCACCTTCTCGTTTTCATTTCAGTTCTCTCTCTTGCACTTGCAGGAGAAAGCCATGCATCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTCTTCGAGAACTTCTAAAGCCTGGACCTCAAGGTGTTGAAATTGATGACCACCCCATGGAAATTGATGACACACAATACCCAAAAACATTTTTCTATGAACATGAACTTTATCCTGGCAAAACAATGAAAGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAGGTATGTGTAAAGAAAGCAGCTGCAGAAGGAGAACAAAAATTTTGTGCAAAATCCTTAGGAACATTAATCGGATTTGCAATTTCTAAGCTTGGAAAAAATATTCAAGCACTTTCAAGTTCCTTCATAGATAAGCATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTTATGTGTCATAGGTTGAATTTCCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCTAATGATGGAACAAAAACTCAAGCTCTAGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGTTGCAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTTTGTCATTTCCTTGGAAATAAGGCTCTTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa0256730

MGFHHLLVFISVLSLALAGESHASLPEEEYWEAVWPNTPIPTSLRELLKPGPQGVEIDDHPMEIDDTQYPKTFFYEHELYPGKTMKVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLIGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLQQIMKADPGSKPVCHFLGNKALLWVPNLGLDNAYGANVAV*