AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1385290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1385290 MtrunA17_Chr2g0318901 94.872 273 14 0 1 273 1 273 0.0 548
Msa1385290 MtrunA17_Chr2g0318871 83.516 273 45 0 1 273 1 273 2.40e-176 486
Msa1385290 MtrunA17_Chr2g0318931 63.941 269 88 5 1 261 5 272 1.11e-123 353
Msa1385290 MtrunA17_Chr8g0355861 45.149 268 132 7 1 260 1 261 4.08e-78 238
Msa1385290 MtrunA17_Chr8g0355321 37.540 309 129 6 10 260 1 303 1.49e-73 227
Msa1385290 MtrunA17_Chr1g0168781 42.435 271 142 6 1 264 1 264 3.16e-72 222
Msa1385290 MtrunA17_Chr8g0355871 45.918 196 97 3 71 259 29 222 1.20e-60 192
Msa1385290 MtrunA17_Chr8g0355311 49.412 170 79 2 96 265 34 196 7.79e-58 183
Msa1385290 MtrunA17_Chr8g0355841 53.165 158 73 1 111 268 362 518 1.33e-57 192
Msa1385290 MtrunA17_Chr8g0355331 45.882 170 85 2 97 266 266 428 1.56e-53 179
Msa1385290 MtrunA17_Chr8g0355831 55.333 150 66 1 111 260 204 352 1.16e-51 172
Msa1385290 MtrunA17_Chr3g0139431 31.889 323 145 12 6 259 5 321 1.60e-44 153
Msa1385290 MtrunA17_Chr1g0147261 33.663 303 149 12 1 257 1 297 2.10e-44 152
Msa1385290 MtrunA17_Chr1g0147311 28.746 327 158 9 5 258 30 354 1.89e-39 140
Msa1385290 MtrunA17_Chr1g0147331 29.538 325 153 11 5 258 4 323 3.10e-38 136
Msa1385290 MtrunA17_Chr1g0147241 30.671 313 158 12 1 257 1 310 1.02e-37 135
Msa1385290 MtrunA17_Chr1g0146631 28.923 325 155 11 5 258 4 323 7.25e-37 133
Msa1385290 MtrunA17_Chr1g0147291 32.456 228 126 9 57 258 40 265 5.96e-31 116
Msa1385290 MtrunA17_Chr1g0147301 32.618 233 123 10 57 258 54 283 7.58e-31 116
Msa1385290 MtrunA17_Chr1g0147271 34.579 214 111 7 73 258 171 383 3.17e-30 116
Msa1385290 MtrunA17_Chr1g0147321 32.456 228 126 9 56 257 10 235 3.78e-30 114
Msa1385290 MtrunA17_Chr1g0147251 31.278 227 123 6 58 257 20 240 5.99e-30 112
Msa1385290 MtrunA17_Chr3g0144421 28.879 232 130 10 59 259 75 302 3.25e-19 85.5
Msa1385290 MtrunA17_Chr4g0034821 29.778 225 116 11 73 261 75 293 5.11e-18 82.0
Msa1385290 MtrunA17_Chr3g0138231 31.250 144 89 4 128 261 125 268 2.18e-15 74.3
Msa1385290 MtrunA17_Chr4g0034731 32.867 143 84 5 129 259 290 432 1.50e-14 73.2
Msa1385290 MtrunA17_Chr3g0144451 32.639 144 87 5 128 261 134 277 1.84e-14 71.6
Msa1385290 MtrunA17_Chr4g0034721 34.266 143 82 6 129 259 434 576 2.76e-14 72.4
Msa1385290 MtrunA17_Chr4g0076231 26.897 145 95 5 123 257 13 156 7.69e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1385290 AT5G25610.1 33.036 224 121 8 63 257 166 389 4.08e-29 114
Msa1385290 AT1G49320.1 35.135 148 81 5 126 258 131 278 1.23e-16 78.2

Find 65 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTCATTTCCTTGGAAATA+AGG 0.195752 tig0020728:+30379 Msa1385290:CDS
GCAAAGATTGTGCCTCTTTA+GGG 0.226798 tig0020728:-30588 None:intergenic
AGCAAAGATTGTGCCTCTTT+AGG 0.246185 tig0020728:-30589 None:intergenic
GACAATAAAATACAACCTTT+TGG 0.266567 tig0020728:-30202 None:intergenic
ATTGAAATCAGAAAATTTAG+AGG 0.297761 tig0020728:+30820 Msa1385290:three_prime_UTR
CGTCCCTTTGCACAACCTTA+TGG 0.298036 tig0020728:+29915 Msa1385290:CDS
CACTGCCTGAAGAAGAATAT+TGG 0.300262 tig0020728:+28720 Msa1385290:CDS
TGGTGCCAATGTTGCTGTTT+AGG 0.323097 tig0020728:+30442 Msa1385290:CDS
GCTTCCCAATATTCTTCTTC+AGG 0.355894 tig0020728:-28725 None:intergenic
AAGAATATTGGGAAGCTGTT+TGG 0.356329 tig0020728:+28732 Msa1385290:CDS
GCATTGTCCAAACCTAAATT+AGG 0.363667 tig0020728:-30416 None:intergenic
AAATCCTTAGGAACATTAAT+CGG 0.372556 tig0020728:+30047 Msa1385290:CDS
GTTTGTCATGCTGATACTTC+AGG 0.375794 tig0020728:+30299 Msa1385290:CDS
GGATTTGCAATTTCTAAGCT+TGG 0.386794 tig0020728:+30068 Msa1385290:CDS
TATGAACATGAACTTTATCC+TGG 0.401999 tig0020728:+29864 Msa1385290:CDS
AAACCTGTTTGTCATTTCCT+TGG 0.406653 tig0020728:+30371 Msa1385290:CDS
GATGTGCAGCTTGCACTTGC+AGG 0.411503 tig0020728:+28683 Msa1385290:intron
ACTGCCTGAAGAAGAATATT+GGG 0.412787 tig0020728:+28721 Msa1385290:CDS
TCTAGCTTAATTAGCTAGAA+AGG 0.415491 tig0020728:-30540 None:intergenic
ATAGAGTCAGTGCAAAATCT+TGG 0.422753 tig0020728:+30146 Msa1385290:CDS
ATGGGTACCTAATTTAGGTT+TGG 0.424424 tig0020728:+30409 Msa1385290:CDS
TTGTGTGTCATCAATTTCCA+TGG 0.427694 tig0020728:-29823 None:intergenic
AGAAAGCAGTTATGTGTCAT+AGG 0.437124 tig0020728:+30171 Msa1385290:CDS
TTGTTCATGCTTATCTATGA+AGG 0.440092 tig0020728:-30117 None:intergenic
AAATAACAACCTTGAGGTCC+AGG 0.452077 tig0020728:-28797 None:intergenic
TAGAAGTTCTCGAAGAGAAG+TGG 0.456535 tig0020728:-28771 None:intergenic
TTTCCAAGGAAATGACAAAC+AGG 0.462441 tig0020728:-30374 None:intergenic
CTAGCTAATTAAGCTAGAGT+TGG 0.466469 tig0020728:+30545 Msa1385290:three_prime_UTR
CATGTTATAATATGATGAAA+TGG 0.470529 tig0020728:+30628 Msa1385290:three_prime_UTR
AACCTTATGGTGTTTATACA+TGG 0.471062 tig0020728:+29928 Msa1385290:CDS
CTTCTTCAGGCAGTGATGCA+TGG 0.473820 tig0020728:-28712 None:intergenic
GAAGTGGGAATGGGAGTGTT+GGG 0.474967 tig0020728:-28755 None:intergenic
ACACCATAAGGTTGTGCAAA+GGG 0.481077 tig0020728:-29918 None:intergenic
TTAGGTTTGGACAATGCCTA+TGG 0.494021 tig0020728:+30422 Msa1385290:CDS
TTCTCGAAGAGAAGTGGGAA+TGG 0.495855 tig0020728:-28765 None:intergenic
CTTCGAGAACTTCTAAAGCC+TGG 0.497324 tig0020728:+28779 Msa1385290:CDS
ATAAAATAGCCTTATTTCCA+AGG 0.498608 tig0020728:-30388 None:intergenic
ATCCCATGGGTTGTCCATGA+TGG 0.500990 tig0020728:-30473 None:intergenic
ATCCATGTATAAACACCATA+AGG 0.501910 tig0020728:-29930 None:intergenic
AGAAGTGGGAATGGGAGTGT+TGG 0.505103 tig0020728:-28756 None:intergenic
GTTCCATCATTAGCCACCAA+TGG 0.510862 tig0020728:-30257 None:intergenic
TCATAGGTTGAATTTCCAAA+AGG 0.512621 tig0020728:+30187 Msa1385290:CDS
CAGATTATGAAAGCTGATCC+TGG 0.522799 tig0020728:+30344 Msa1385290:CDS
ATTTATGTTACACAATCCCA+TGG 0.523591 tig0020728:-30487 None:intergenic
AAATCCGATTAATGTTCCTA+AGG 0.524830 tig0020728:-30051 None:intergenic
GTTCCATTGGTGGCTAATGA+TGG 0.535070 tig0020728:+30254 Msa1385290:CDS
TGTGTGTCATCAATTTCCAT+GGG 0.537514 tig0020728:-29822 None:intergenic
TCTCGAAGAGAAGTGGGAAT+GGG 0.548515 tig0020728:-28764 None:intergenic
CTTCTAAAGCCTGGACCTCA+AGG 0.560362 tig0020728:+28788 Msa1385290:CDS
AACAGCAACATTGGCACCAT+AGG 0.567015 tig0020728:-30438 None:intergenic
AGAAGTTCTCGAAGAGAAGT+GGG 0.572137 tig0020728:-28770 None:intergenic
TCTCCATCATGGACAACCCA+TGG 0.575120 tig0020728:+30470 Msa1385290:three_prime_UTR
GTAAAGAAAGCAGCTGCAGA+AGG 0.576977 tig0020728:+30005 Msa1385290:CDS
GTACTCCTAAACAGCAACAT+TGG 0.581094 tig0020728:-30447 None:intergenic
AGAAGGATACACATTTAATG+AGG 0.587845 tig0020728:+29977 Msa1385290:CDS
TTTAGGAGTACTCTCCATCA+TGG 0.588077 tig0020728:+30459 Msa1385290:exon
CTCCATCATGGACAACCCAT+GGG 0.596865 tig0020728:+30471 Msa1385290:three_prime_UTR
TGTCATCAATTTCCATGGGG+TGG 0.605445 tig0020728:-29818 None:intergenic
GAACTGAAATGAAAACGAGA+AGG 0.613747 tig0020728:-28281 None:intergenic
TTTATGTTACACAATCCCAT+GGG 0.616443 tig0020728:-30486 None:intergenic
TGACAAACAGGTTTACTACC+AGG 0.616609 tig0020728:-30362 None:intergenic
AAAATATCATCGATAAGACT+CGG 0.638308 tig0020728:-30715 None:intergenic
AACACCATAAGGTTGTGCAA+AGG 0.645055 tig0020728:-29919 None:intergenic
TGAAATTGATGACCACCCCA+TGG 0.663021 tig0020728:+29806 Msa1385290:CDS
GTGTGTCATCAATTTCCATG+GGG 0.716189 tig0020728:-29821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCACAATATTAATTAATAT+AGG - tig0020728:28899-28918 None:intergenic 10.0%
!!! TAATCCTTTATTAATTTTAT+TGG + tig0020728:29004-29023 Msa1385290:intron 10.0%
!!! CATTTAAAATATAAAAAAGT+TGG + tig0020728:29257-29276 Msa1385290:intron 10.0%
!! ACATTATTAATTTATTTGTT+AGG - tig0020728:29498-29517 None:intergenic 10.0%
!! TGATCCAATAAAATTAATAA+AGG - tig0020728:29011-29030 None:intergenic 15.0%
!! ATCGATATTTACTTTATATT+TGG + tig0020728:29222-29241 Msa1385290:intron 15.0%
!!! TATTTTAAATGTATTAGGTT+GGG - tig0020728:29248-29267 None:intergenic 15.0%
!!! ATATTTTAAATGTATTAGGT+TGG - tig0020728:29249-29268 None:intergenic 15.0%
!! TATTTGTCATATAAATTGAA+TGG - tig0020728:29389-29408 None:intergenic 15.0%
!! TTTATTAGTTAAAATTCAGA+TGG + tig0020728:29626-29645 Msa1385290:intron 15.0%
!! TTATTAGTTAAAATTCAGAT+GGG + tig0020728:29627-29646 Msa1385290:intron 15.0%
!!! TAACACATATTCTTTTTTAT+TGG - tig0020728:29706-29725 None:intergenic 15.0%
!! AAAGAATATGTGTTAAATTA+TGG + tig0020728:29711-29730 Msa1385290:intron 15.0%
!!! TTTCGTAAGTAAATATTTTT+AGG + tig0020728:30677-30696 Msa1385290:three_prime_UTR 15.0%
!!! TTCGTAAGTAAATATTTTTA+GGG + tig0020728:30678-30697 Msa1385290:three_prime_UTR 15.0%
!! AATAACATGTCATAAAAAAA+AGG + tig0020728:30749-30768 Msa1385290:three_prime_UTR 15.0%
!! TTAAACAACAACCTAAAATA+TGG + tig0020728:28403-28422 Msa1385290:intron 20.0%
!!! TACTTTAATTGCCATATTTT+AGG - tig0020728:28417-28436 None:intergenic 20.0%
!! ATTATTACTTACATATGTTG+AGG + tig0020728:28453-28472 Msa1385290:intron 20.0%
!! TTATTACTTACATATGTTGA+GGG + tig0020728:28454-28473 Msa1385290:intron 20.0%
!! AAAATTAAATAACAACCTTG+AGG - tig0020728:28806-28825 None:intergenic 20.0%
!!! TCAGAAAAGGTAAATTTTAT+GGG + tig0020728:28946-28965 Msa1385290:intron 20.0%
!! TTATATGACAAATAATTCCA+AGG + tig0020728:29395-29414 Msa1385290:intron 20.0%
!! TATATGACAAATAATTCCAA+GGG + tig0020728:29396-29415 Msa1385290:intron 20.0%
!!! AATTTATTTGTTAGGATGAA+AGG - tig0020728:29490-29509 None:intergenic 20.0%
!! TCTCTCAAGTTATATATTAT+TGG - tig0020728:29524-29543 None:intergenic 20.0%
!!! TATTTTAAACCCATGTATTA+TGG + tig0020728:29774-29793 Msa1385290:intron 20.0%
!!! GTTCATAGAAAAATGTTTTT+GGG - tig0020728:29851-29870 None:intergenic 20.0%
!!! TGTTCATAGAAAAATGTTTT+TGG - tig0020728:29852-29871 None:intergenic 20.0%
!! ATTAAAGACATTGAAAAAGA+AGG + tig0020728:29960-29979 Msa1385290:CDS 20.0%
!!! TTTTATTGTCATGAAATTCA+TGG + tig0020728:30215-30234 Msa1385290:CDS 20.0%
!! CATGTTATAATATGATGAAA+TGG + tig0020728:30628-30647 Msa1385290:three_prime_UTR 20.0%
!!! TTTTTAGGGTTAAATATGTT+TGG + tig0020728:30692-30711 Msa1385290:three_prime_UTR 20.0%
!! ATTGAAATCAGAAAATTTAG+AGG + tig0020728:30820-30839 Msa1385290:three_prime_UTR 20.0%
! TAAGTTTATAGTACATCAGT+TGG - tig0020728:28386-28405 None:intergenic 25.0%
! GTAAGAAATAAACTCAGAAA+AGG + tig0020728:28933-28952 Msa1385290:intron 25.0%
!! CTCAGAAAAGGTAAATTTTA+TGG + tig0020728:28945-28964 Msa1385290:intron 25.0%
! AGAGATGAAAAACTAATAAG+GGG - tig0020728:29062-29081 None:intergenic 25.0%
! CAGAGATGAAAAACTAATAA+GGG - tig0020728:29063-29082 None:intergenic 25.0%
! TCAGAGATGAAAAACTAATA+AGG - tig0020728:29064-29083 None:intergenic 25.0%
! ACAAAATTAATACTCTCCTT+CGG - tig0020728:29114-29133 None:intergenic 25.0%
!!! AAGGAGAGTATTAATTTTGT+AGG + tig0020728:29114-29133 Msa1385290:intron 25.0%
! TCATGTGAGTTTCATAAATT+TGG - tig0020728:29657-29676 None:intergenic 25.0%
! TTTATGAAACTCACATGATT+TGG + tig0020728:29659-29678 Msa1385290:intron 25.0%
!! AAATTTTGTGCAAAATCCTT+AGG + tig0020728:30035-30054 Msa1385290:CDS 25.0%
! AAATCCTTAGGAACATTAAT+CGG + tig0020728:30047-30066 Msa1385290:CDS 25.0%
!! GACAATAAAATACAACCTTT+TGG - tig0020728:30205-30224 None:intergenic 25.0%
!!! ATAAAATAGCCTTATTTCCA+AGG - tig0020728:30391-30410 None:intergenic 25.0%
!!! TTGGAAATAAGGCTATTTTA+TGG + tig0020728:30390-30409 Msa1385290:CDS 25.0%
!!! TGGAAATAAGGCTATTTTAT+GGG + tig0020728:30391-30410 Msa1385290:CDS 25.0%
!! ATTTTATGGGTACCTAATTT+AGG + tig0020728:30404-30423 Msa1385290:CDS 25.0%
! AAAATATCATCGATAAGACT+CGG - tig0020728:30718-30737 None:intergenic 25.0%
!! GTCTTATCGATGATATTTTT+AGG + tig0020728:30719-30738 Msa1385290:three_prime_UTR 25.0%
AAGAGTTCTTAAGTGTTTAG+AGG - tig0020728:28351-28370 None:intergenic 30.0%
TCTAAACACTTAAGAACTCT+TGG + tig0020728:28350-28369 Msa1385290:intron 30.0%
AGGTTGACAAATTTGAATGT+TGG - tig0020728:29144-29163 None:intergenic 30.0%
! TGACTCTATAATTTTTGCCT+AGG + tig0020728:29320-29339 Msa1385290:intron 30.0%
TAAATTTAGAAAGAGACCCT+TGG - tig0020728:29415-29434 None:intergenic 30.0%
! GAGAGATGTTATGATTTTTC+TGG + tig0020728:29540-29559 Msa1385290:intron 30.0%
TGTGAGTTTCATAAATTTGG+AGG - tig0020728:29654-29673 None:intergenic 30.0%
TATGAACATGAACTTTATCC+TGG + tig0020728:29864-29883 Msa1385290:CDS 30.0%
AACCTTATGGTGTTTATACA+TGG + tig0020728:29928-29947 Msa1385290:CDS 30.0%
ATCCATGTATAAACACCATA+AGG - tig0020728:29933-29952 None:intergenic 30.0%
AGAAGGATACACATTTAATG+AGG + tig0020728:29977-29996 Msa1385290:CDS 30.0%
AAATCCGATTAATGTTCCTA+AGG - tig0020728:30054-30073 None:intergenic 30.0%
TTGTTCATGCTTATCTATGA+AGG - tig0020728:30120-30139 None:intergenic 30.0%
! TCATAGGTTGAATTTCCAAA+AGG + tig0020728:30187-30206 Msa1385290:CDS 30.0%
! TTGTCATTTCCTTGGAAATA+AGG + tig0020728:30379-30398 Msa1385290:CDS 30.0%
TTTATGTTACACAATCCCAT+GGG - tig0020728:30489-30508 None:intergenic 30.0%
ATTTATGTTACACAATCCCA+TGG - tig0020728:30490-30509 None:intergenic 30.0%
! TCTAGCTTAATTAGCTAGAA+AGG - tig0020728:30543-30562 None:intergenic 30.0%
! TACTTTTATGTACCCTAAAG+AGG + tig0020728:30576-30595 Msa1385290:three_prime_UTR 30.0%
GAACTGAAATGAAAACGAGA+AGG - tig0020728:28284-28303 None:intergenic 35.0%
!!! GAAATGAAAGGTTTTAGAGC+AGG - tig0020728:28322-28341 None:intergenic 35.0%
!! TAGAGGATGATGGAAATGAA+AGG - tig0020728:28334-28353 None:intergenic 35.0%
CTTAAGTGTTTAGAGGATGA+TGG - tig0020728:28344-28363 None:intergenic 35.0%
ACTGCCTGAAGAAGAATATT+GGG + tig0020728:28721-28740 Msa1385290:CDS 35.0%
AAGAATATTGGGAAGCTGTT+TGG + tig0020728:28732-28751 Msa1385290:CDS 35.0%
!! TCTCTGAAGCTGTAATATAG+TGG + tig0020728:29077-29096 Msa1385290:intron 35.0%
!! TTGTTAGGATGAAAGGTGTT+TGG - tig0020728:29483-29502 None:intergenic 35.0%
!! TATGGTGTCACAGTCAAAAT+TGG + tig0020728:29729-29748 Msa1385290:intron 35.0%
TGTGTGTCATCAATTTCCAT+GGG - tig0020728:29825-29844 None:intergenic 35.0%
TTGTGTGTCATCAATTTCCA+TGG - tig0020728:29826-29845 None:intergenic 35.0%
GGATTTGCAATTTCTAAGCT+TGG + tig0020728:30068-30087 Msa1385290:CDS 35.0%
ATAGAGTCAGTGCAAAATCT+TGG + tig0020728:30146-30165 Msa1385290:CDS 35.0%
AGAAAGCAGTTATGTGTCAT+AGG + tig0020728:30171-30190 Msa1385290:CDS 35.0%
! TCATGGAACAACAGCTTTTA+TGG + tig0020728:30232-30251 Msa1385290:CDS 35.0%
! AACAGCTTTTATGGTTCCAT+TGG + tig0020728:30241-30260 Msa1385290:CDS 35.0%
! AAACCTGTTTGTCATTTCCT+TGG + tig0020728:30371-30390 Msa1385290:CDS 35.0%
TTTCCAAGGAAATGACAAAC+AGG - tig0020728:30377-30396 None:intergenic 35.0%
! ATGGGTACCTAATTTAGGTT+TGG + tig0020728:30409-30428 Msa1385290:CDS 35.0%
GCATTGTCCAAACCTAAATT+AGG - tig0020728:30419-30438 None:intergenic 35.0%
CTAGCTAATTAAGCTAGAGT+TGG + tig0020728:30545-30564 Msa1385290:three_prime_UTR 35.0%
ACTTACATATGTTGAGGGTG+TGG + tig0020728:28459-28478 Msa1385290:intron 40.0%
CACTGCCTGAAGAAGAATAT+TGG + tig0020728:28720-28739 Msa1385290:CDS 40.0%
GCTTCCCAATATTCTTCTTC+AGG - tig0020728:28728-28747 None:intergenic 40.0%
AGAAGTTCTCGAAGAGAAGT+GGG - tig0020728:28773-28792 None:intergenic 40.0%
TAGAAGTTCTCGAAGAGAAG+TGG - tig0020728:28774-28793 None:intergenic 40.0%
AAATAACAACCTTGAGGTCC+AGG - tig0020728:28800-28819 None:intergenic 40.0%
AGTGGTATTGTTGAATCCGA+AGG + tig0020728:29095-29114 Msa1385290:intron 40.0%
! CCAGGAATTTTGTAAAGCCT+AGG - tig0020728:29340-29359 None:intergenic 40.0%
CCTAGGCTTTACAAAATTCC+TGG + tig0020728:29337-29356 Msa1385290:intron 40.0%
TACAGCACCATACTGTACAT+CGG + tig0020728:29449-29468 Msa1385290:intron 40.0%
AAACCCATGTATTATGGCAC+AGG + tig0020728:29780-29799 Msa1385290:intron 40.0%
ACACCTGTGCCATAATACAT+GGG - tig0020728:29786-29805 None:intergenic 40.0%
AACACCTGTGCCATAATACA+TGG - tig0020728:29787-29806 None:intergenic 40.0%
GTGTGTCATCAATTTCCATG+GGG - tig0020728:29824-29843 None:intergenic 40.0%
!! TGCACTTTCATTGTTTTGCC+AGG - tig0020728:29885-29904 None:intergenic 40.0%
ACACCATAAGGTTGTGCAAA+GGG - tig0020728:29921-29940 None:intergenic 40.0%
AACACCATAAGGTTGTGCAA+AGG - tig0020728:29922-29941 None:intergenic 40.0%
! AGCTTTTATGGTTCCATTGG+TGG + tig0020728:30244-30263 Msa1385290:CDS 40.0%
GTTTGTCATGCTGATACTTC+AGG + tig0020728:30299-30318 Msa1385290:CDS 40.0%
CAGATTATGAAAGCTGATCC+TGG + tig0020728:30344-30363 Msa1385290:CDS 40.0%
TGACAAACAGGTTTACTACC+AGG - tig0020728:30365-30384 None:intergenic 40.0%
TTAGGTTTGGACAATGCCTA+TGG + tig0020728:30422-30441 Msa1385290:CDS 40.0%
GTACTCCTAAACAGCAACAT+TGG - tig0020728:30450-30469 None:intergenic 40.0%
!! TTTAGGAGTACTCTCCATCA+TGG + tig0020728:30459-30478 Msa1385290:exon 40.0%
GCAAAGATTGTGCCTCTTTA+GGG - tig0020728:30591-30610 None:intergenic 40.0%
AGCAAAGATTGTGCCTCTTT+AGG - tig0020728:30592-30611 None:intergenic 40.0%
TCTCGAAGAGAAGTGGGAAT+GGG - tig0020728:28767-28786 None:intergenic 45.0%
TTCTCGAAGAGAAGTGGGAA+TGG - tig0020728:28768-28787 None:intergenic 45.0%
!! CTTCGAGAACTTCTAAAGCC+TGG + tig0020728:28779-28798 Msa1385290:CDS 45.0%
ACCATACTGTACATCGGTAG+AGG + tig0020728:29455-29474 Msa1385290:intron 45.0%
TCCTCTACCGATGTACAGTA+TGG - tig0020728:29459-29478 None:intergenic 45.0%
! TGAAATTGATGACCACCCCA+TGG + tig0020728:29806-29825 Msa1385290:CDS 45.0%
TGTCATCAATTTCCATGGGG+TGG - tig0020728:29821-29840 None:intergenic 45.0%
GTAAAGAAAGCAGCTGCAGA+AGG + tig0020728:30005-30024 Msa1385290:CDS 45.0%
! GTTCCATTGGTGGCTAATGA+TGG + tig0020728:30254-30273 Msa1385290:CDS 45.0%
GTTCCATCATTAGCCACCAA+TGG - tig0020728:30260-30279 None:intergenic 45.0%
AACAGCAACATTGGCACCAT+AGG - tig0020728:30441-30460 None:intergenic 45.0%
!! TGGTGCCAATGTTGCTGTTT+AGG + tig0020728:30442-30461 Msa1385290:CDS 45.0%
!!! TTTTATATTTTAAATGTATT+AGG - tig0020728:29253-29272 None:intergenic 5.0%
!!! ATTTAAAATATAAAAAAGTT+GGG + tig0020728:29258-29277 Msa1385290:intron 5.0%
!! AAAATAAAAATTATACAATA+AGG - tig0020728:30660-30679 None:intergenic 5.0%
CTTCTTCAGGCAGTGATGCA+TGG - tig0020728:28715-28734 None:intergenic 50.0%
!! GAAGTGGGAATGGGAGTGTT+GGG - tig0020728:28758-28777 None:intergenic 50.0%
! AGAAGTGGGAATGGGAGTGT+TGG - tig0020728:28759-28778 None:intergenic 50.0%
!! CTTCTAAAGCCTGGACCTCA+AGG + tig0020728:28788-28807 Msa1385290:CDS 50.0%
AAAATTCCTGGCTCCACCAC+TGG + tig0020728:29349-29368 Msa1385290:intron 50.0%
CGTCCCTTTGCACAACCTTA+TGG + tig0020728:29915-29934 Msa1385290:CDS 50.0%
TCTCCATCATGGACAACCCA+TGG + tig0020728:30470-30489 Msa1385290:three_prime_UTR 50.0%
CTCCATCATGGACAACCCAT+GGG + tig0020728:30471-30490 Msa1385290:three_prime_UTR 50.0%
! ATCCCATGGGTTGTCCATGA+TGG - tig0020728:30476-30495 None:intergenic 50.0%
GATGTGCAGCTTGCACTTGC+AGG + tig0020728:28683-28702 Msa1385290:intron 55.0%
TGTCAACCTCTGCTGCAGCC+AGG + tig0020728:29155-29174 Msa1385290:intron 60.0%
GTCAACCTCTGCTGCAGCCA+GGG + tig0020728:29156-29175 Msa1385290:intron 60.0%
TCAACCTCTGCTGCAGCCAG+GGG + tig0020728:29157-29176 Msa1385290:intron 60.0%
ATTGAGCCCAGACGAGTGCC+CGG - tig0020728:29204-29223 None:intergenic 60.0%
TTGAGCCCAGACGAGTGCCC+GGG - tig0020728:29203-29222 None:intergenic 65.0%
CCTACAGCTGCAGCCAGTGG+TGG - tig0020728:29365-29384 None:intergenic 65.0%
! CCACCACTGGCTGCAGCTGT+AGG + tig0020728:29362-29381 Msa1385290:intron 65.0%
GGTCCTACAGCTGCAGCCAG+TGG - tig0020728:29368-29387 None:intergenic 65.0%
TTCGCCCCTGGCTGCAGCAG+AGG - tig0020728:29164-29183 None:intergenic 70.0%
TCTGCTGCAGCCAGGGGCGA+AGG + tig0020728:29163-29182 Msa1385290:intron 70.0%
! CTGCAGCCAGTGGTGGAGCC+AGG - tig0020728:29358-29377 None:intergenic 70.0%
!! CCAGGGGCGAAGGCACCACC+CGG + tig0020728:29173-29192 Msa1385290:intron 75.0%
! AAGGCACCACCCGGCGAGCC+CGG + tig0020728:29182-29201 Msa1385290:intron 75.0%
AGACGAGTGCCCGGGCTCGC+CGG - tig0020728:29195-29214 None:intergenic 75.0%
GCGAGCCCGGGCACTCGTCT+GGG + tig0020728:29195-29214 Msa1385290:intron 75.0%
! CCGGGTGGTGCCTTCGCCCC+TGG - tig0020728:29176-29195 None:intergenic 80.0%
! AGGCACCACCCGGCGAGCCC+GGG + tig0020728:29183-29202 Msa1385290:intron 80.0%
! GACGAGTGCCCGGGCTCGCC+GGG - tig0020728:29194-29213 None:intergenic 80.0%
! GGCGAGCCCGGGCACTCGTC+TGG + tig0020728:29194-29213 Msa1385290:intron 80.0%
! GAGTGCCCGGGCTCGCCGGG+TGG - tig0020728:29191-29210 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
tig0020728 gene 28267 30860 28267 ID=Msa1385290;Name=Msa1385290
tig0020728 mRNA 28267 30860 28267 ID=Msa1385290-mRNA-1;Parent=Msa1385290;Name=Msa1385290-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|1|0.66|1|1|1|3|397|273
tig0020728 exon 28267 28308 28267 ID=Msa1385290-mRNA-1:exon:9522;Parent=Msa1385290-mRNA-1
tig0020728 exon 28692 28809 28692 ID=Msa1385290-mRNA-1:exon:9523;Parent=Msa1385290-mRNA-1
tig0020728 exon 29802 30860 29802 ID=Msa1385290-mRNA-1:exon:9524;Parent=Msa1385290-mRNA-1
tig0020728 CDS 28267 28308 28267 ID=Msa1385290-mRNA-1:cds;Parent=Msa1385290-mRNA-1
tig0020728 CDS 28692 28809 28692 ID=Msa1385290-mRNA-1:cds;Parent=Msa1385290-mRNA-1
tig0020728 CDS 29802 30463 29802 ID=Msa1385290-mRNA-1:cds;Parent=Msa1385290-mRNA-1
tig0020728 three_prime_UTR 30464 30860 30464 ID=Msa1385290-mRNA-1:three_prime_utr;Parent=Msa1385290-mRNA-1
Gene Sequence

>Msa1385290

ATGGGATTTCATCACCTTCTCGTTTTCATTTCAGTTCTCTCTCTTGCACTTGCAGGAGAAAGCCATGCATCACTGCCTGAAGAAGAATATTGGGAAGCTGTTTGGCCCAACACTCCCATTCCCACTTCTCTTCGAGAACTTCTAAAGCCTGGACCTCAAGGTGTTGAAATTGATGACCACCCCATGGAAATTGATGACACACAATACCCAAAAACATTTTTCTATGAACATGAACTTTATCCTGGCAAAACAATGAAAGTGCAATTCAGCAAGCGTCCCTTTGCACAACCTTATGGTGTTTATACATGGATGCGTGAAATTAAAGACATTGAAAAAGAAGGATACACATTTAATGAGGTATGTGTAAAGAAAGCAGCTGCAGAAGGAGAACAAAAATTTTGTGCAAAATCCTTAGGAACATTAATCGGATTTGCAATTTCTAAGCTTGGAAAAAATATTCAAGCACTTTCAAGTTCCTTCATAGATAAGCATGAACAATACAAAATAGAGTCAGTGCAAAATCTTGGAGAGAAAGCAGTTATGTGTCATAGGTTGAATTTCCAAAAGGTTGTATTTTATTGTCATGAAATTCATGGAACAACAGCTTTTATGGTTCCATTGGTGGCTAATGATGGAACAAAAACTCAAGCTCTAGCTGTTTGTCATGCTGATACTTCAGGCATGAATCATGAAATGTTGCAACAGATTATGAAAGCTGATCCTGGTAGTAAACCTGTTTGTCATTTCCTTGGAAATAAGGCTATTTTATGGGTACCTAATTTAGGTTTGGACAATGCCTATGGTGCCAATGTTGCTGTTTAG
Protein sequence

>Msa1385290

MGFHHLLVFISVLSLALAGESHASLPEEEYWEAVWPNTPIPTSLRELLKPGPQGVEIDDHPMEIDDTQYPKTFFYEHELYPGKTMKVQFSKRPFAQPYGVYTWMREIKDIEKEGYTFNEVCVKKAAAEGEQKFCAKSLGTLIGFAISKLGKNIQALSSSFIDKHEQYKIESVQNLGEKAVMCHRLNFQKVVFYCHEIHGTTAFMVPLVANDGTKTQALAVCHADTSGMNHEMLQQIMKADPGSKPVCHFLGNKAILWVPNLGLDNAYGANVAV*