AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0262420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262420 MtrunA17_Chr2g0325041 81.633 49 9 0 4 52 3 51 7.38e-23 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGGTCTGATACGGGTTT+TGG 0.179646 2_2:+61353665 Msa0262420:five_prime_UTR
TCTGCAAACAAACTGGTTTA+TGG 0.217646 2_2:+61353112 Msa0262420:five_prime_UTR
GAGAGGATGAGGAAGGTTTC+TGG 0.226601 2_2:+61353647 Msa0262420:five_prime_UTR
TGCCGAGGGAGGAGTGTCTT+AGG 0.240149 2_2:+61353594 Msa0262420:five_prime_UTR
CTTTGAAGCGTTGTTGGTTT+TGG 0.257075 2_2:-61353204 None:intergenic
TCTTTCTCGCCGTTCAAAAC+CGG 0.266026 2_2:-61353512 None:intergenic
TTGTGAATCATTACAAATTT+CGG 0.267761 2_2:-61355823 None:intergenic
TGTTGGATCGGGTTGGGTTT+GGG 0.289585 2_2:-61353377 None:intergenic
GTAGATGTGGTTGAAGTTAA+TGG 0.296091 2_2:-61353156 None:intergenic
TTGTTGGATCGGGTTGGGTT+TGG 0.297089 2_2:-61353378 None:intergenic
TAATGATTCACAAGATGATT+TGG 0.306096 2_2:+61355833 Msa0262420:CDS
ATGTGAAATGGGTTTGGGTG+TGG 0.311571 2_2:-61353330 None:intergenic
TCATGATCATCCACTAGAAT+TGG 0.332847 2_2:-61353696 None:intergenic
TTGGGCTGTGATTGTGCGGG+TGG 0.336985 2_2:-61353359 None:intergenic
GGGTGGGGTTGGATGTGAAA+TGG 0.337055 2_2:-61353342 None:intergenic
CGGTTACAAATTGCGATGTT+AGG 0.340431 2_2:-61355803 None:intergenic
GGTTGGATGTGAAATGGGTT+TGG 0.349429 2_2:-61353336 None:intergenic
GGTGGGGTTGGATGTGAAAT+GGG 0.353408 2_2:-61353341 None:intergenic
TTCAATCTCGCTTCGTCCGT+TGG 0.354260 2_2:+61353451 Msa0262420:five_prime_UTR
CTTTCTCGCCGTTCAAAACC+GGG 0.355045 2_2:-61353511 None:intergenic
TCGCCGTTCAAAACCGGGTT+CGG 0.362760 2_2:-61353506 None:intergenic
GGTGTTGAGAATCTGTCTTC+TGG 0.363513 2_2:-61355782 None:intergenic
CCTCTCATTCCCACCAGACT+TGG 0.365997 2_2:-61353630 None:intergenic
AATGGGTTTGGGTGTGGGTC+GGG 0.366489 2_2:-61353324 None:intergenic
GTTGGATGTGAAATGGGTTT+GGG 0.371160 2_2:-61353335 None:intergenic
TGTGCCGCCGCATCTTCGTC+CGG 0.384242 2_2:+61353565 Msa0262420:five_prime_UTR
AGGATTCGGCCATGTTTGTT+TGG 0.385651 2_2:-61353293 None:intergenic
AAATGGGTTTGGGTGTGGGT+CGG 0.388508 2_2:-61353325 None:intergenic
AGATCGACCCCGGTCTGAAC+CGG 0.391141 2_2:+61353421 Msa0262420:five_prime_UTR
GTACATATCCTTTGTCCCTC+TGG 0.393138 2_2:+61355704 Msa0262420:CDS
TCAATCTCGCTTCGTCCGTT+GGG 0.395438 2_2:+61353452 Msa0262420:five_prime_UTR
TGTGATTGTGCGGGTGGGGT+TGG 0.395841 2_2:-61353353 None:intergenic
CTCTCTCTCTGCAAACAAAC+TGG 0.399039 2_2:+61353105 Msa0262420:five_prime_UTR
TGGGCTGTGATTGTGCGGGT+GGG 0.405665 2_2:-61353358 None:intergenic
TGTTGTTGTTGGATCGGGTT+GGG 0.408930 2_2:-61353383 None:intergenic
GAAGGTTTCTGGGTCTGATA+CGG 0.416702 2_2:+61353658 Msa0262420:five_prime_UTR
CGGGGTCGATCTGAAGAAAC+TGG 0.419897 2_2:-61353410 None:intergenic
GGACGAAGCGAGATTGAATC+TGG 0.420282 2_2:-61353446 None:intergenic
TGTGAAATGGGTTTGGGTGT+GGG 0.421896 2_2:-61353329 None:intergenic
GTGCCGCCGCATCTTCGTCC+GGG 0.426862 2_2:+61353566 Msa0262420:five_prime_UTR
TCGAATCGGTTCTTTGTCGT+CGG 0.435485 2_2:+61353475 Msa0262420:five_prime_UTR
AGGGTTCTTTGAAGCGTTGT+TGG 0.436197 2_2:-61353210 None:intergenic
GGGCTGTGATTGTGCGGGTG+GGG 0.445432 2_2:-61353357 None:intergenic
TCGTCCGGGGTTTGTGCCGA+GGG 0.450244 2_2:+61353580 Msa0262420:five_prime_UTR
ATCTGGTCCCGGTTCAGACC+GGG 0.453477 2_2:-61353429 None:intergenic
GAAAACTTGCGACCCAAGTC+TGG 0.455303 2_2:+61353617 Msa0262420:five_prime_UTR
AAACTGGAGGCTGTTGTTGT+TGG 0.455936 2_2:-61353394 None:intergenic
AGAGGATGAGGAAGGTTTCT+GGG 0.456615 2_2:+61353648 Msa0262420:five_prime_UTR
CTGTTGTTGTTGGATCGGGT+TGG 0.464894 2_2:-61353384 None:intergenic
CTTCGTCCGTTGGGTCGAAT+CGG 0.470054 2_2:+61353461 Msa0262420:five_prime_UTR
CGAATCGGTTCTTTGTCGTC+GGG 0.470387 2_2:+61353476 Msa0262420:five_prime_UTR
CTGGTGGGAATGAGAGGATG+AGG 0.472518 2_2:+61353636 Msa0262420:five_prime_UTR
GGGTTTGGGCTGTGATTGTG+CGG 0.473346 2_2:-61353363 None:intergenic
CATGATCATCCACTAGAATT+GGG 0.480790 2_2:-61353695 None:intergenic
TCCCTAAGACACTCCTCCCT+CGG 0.483826 2_2:-61353596 None:intergenic
CTCTCATTCCCACCAGACTT+GGG 0.484303 2_2:-61353629 None:intergenic
GAGGCTGTTGTTGTTGGATC+GGG 0.484796 2_2:-61353388 None:intergenic
CGAGAAAGAGAAGAGTTTGC+CGG 0.486001 2_2:+61353526 Msa0262420:five_prime_UTR
TCCTCCCTCGGCACAAACCC+CGG 0.493049 2_2:-61353584 None:intergenic
AAGGTTTCTGGGTCTGATAC+GGG 0.495133 2_2:+61353659 Msa0262420:five_prime_UTR
AAGAAGAAGAAGAAAGAGAA+GGG 0.496336 2_2:-61353229 None:intergenic
GCAAGAAGAAGAAAGAAGGA+AGG 0.498038 2_2:-61353034 None:intergenic
TTCGTCCGGGGTTTGTGCCG+AGG 0.499430 2_2:+61353579 Msa0262420:five_prime_UTR
GGAGGCTGTTGTTGTTGGAT+CGG 0.500195 2_2:-61353389 None:intergenic
GTCGGGTTAAAACGAGGATT+CGG 0.502204 2_2:-61353307 None:intergenic
AGAATACGAAGCGGAGGAGA+AGG 0.505453 2_2:-61353258 None:intergenic
GCCGAGGGAGGAGTGTCTTA+GGG 0.509355 2_2:+61353595 Msa0262420:five_prime_UTR
AAATGCAAGAAGAAGAAAGA+AGG 0.510872 2_2:-61353038 None:intergenic
AATCTGGTCCCGGTTCAGAC+CGG 0.519367 2_2:-61353430 None:intergenic
AGCGAGATTGAATCTGGTCC+CGG 0.526016 2_2:-61353440 None:intergenic
CTAGTGGATGATCATGATCA+TGG 0.538732 2_2:+61353702 Msa0262420:exon
GATCGACCCCGGTCTGAACC+GGG 0.543684 2_2:+61353422 Msa0262420:five_prime_UTR
CCAAGTCTGGTGGGAATGAG+AGG 0.547204 2_2:+61353630 Msa0262420:five_prime_UTR
GGGAATCGACCCAATTCTAG+TGG 0.555420 2_2:+61353686 Msa0262420:five_prime_UTR
AACTTGCGACCCAAGTCTGG+TGG 0.558391 2_2:+61353620 Msa0262420:five_prime_UTR
GGTTTGGGCTGTGATTGTGC+GGG 0.560295 2_2:-61353362 None:intergenic
TGCCGCCGCATCTTCGTCCG+GGG 0.561537 2_2:+61353567 Msa0262420:five_prime_UTR
TCCGGGGTTTGTGCCGAGGG+AGG 0.569379 2_2:+61353583 Msa0262420:five_prime_UTR
TGGGAATGAGAGGATGAGGA+AGG 0.570759 2_2:+61353640 Msa0262420:five_prime_UTR
CAGTTTCTTCAGATCGACCC+CGG 0.573018 2_2:+61353411 Msa0262420:five_prime_UTR
AAGAGTTTGCCGGTGAAAAG+TGG 0.574187 2_2:+61353536 Msa0262420:five_prime_UTR
TGGGTTTGTTATCGTAGATG+TGG 0.577249 2_2:-61353169 None:intergenic
TCGGCCATGTTTGTTTGGTG+CGG 0.579784 2_2:-61353288 None:intergenic
ACAAACCCCGGACGAAGATG+CGG 0.590708 2_2:-61353572 None:intergenic
TCTGGTCCCGGTTCAGACCG+GGG 0.604345 2_2:-61353428 None:intergenic
AAAATTGATGTCAGTTCCAG+AGG 0.606435 2_2:-61355720 None:intergenic
GTGTGGGTCGGGTTAAAACG+AGG 0.611653 2_2:-61353313 None:intergenic
TGTCAGTTCCAGAGGGACAA+AGG 0.616152 2_2:-61355712 None:intergenic
ACTTGCGACCCAAGTCTGGT+GGG 0.619752 2_2:+61353621 Msa0262420:five_prime_UTR
AACCCCGGACGAAGATGCGG+CGG 0.626124 2_2:-61353569 None:intergenic
AACTGCAGAATACGAAGCGG+AGG 0.631412 2_2:-61353264 None:intergenic
GTTTCCGCACCAAACAAACA+TGG 0.648595 2_2:+61353284 Msa0262420:five_prime_UTR
AAAGAACCGATTCGACCCAA+CGG 0.649372 2_2:-61353467 None:intergenic
AAATTGATGTCAGTTCCAGA+GGG 0.658418 2_2:-61355719 None:intergenic
GGTCGATCTGAAGAAACTGG+AGG 0.681748 2_2:-61353407 None:intergenic
GGAAACTGCAGAATACGAAG+CGG 0.759361 2_2:-61353267 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATATGATGAAATTAAT+TGG + chr2_2:61353915-61353934 Msa0262420:intron 10.0%
!! TAAATATGATGAAATTAATT+GGG + chr2_2:61353916-61353935 Msa0262420:intron 10.0%
!! TTTCTAAATTAGTTAACATA+TGG - chr2_2:61353863-61353882 None:intergenic 15.0%
!!! AATCACTTATATAGTATTTT+AGG + chr2_2:61354023-61354042 Msa0262420:intron 15.0%
!! GAATGATAACATTAATTATA+TGG + chr2_2:61354160-61354179 Msa0262420:intron 15.0%
!!! TTTTTTTTTTTTTGGATGAA+GGG - chr2_2:61355167-61355186 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTGGATGA+AGG - chr2_2:61355168-61355187 None:intergenic 15.0%
!!! TTTTCTTGATTGTTCTTTAA+TGG + chr2_2:61353787-61353806 Msa0262420:intron 20.0%
!! CAACAAAATATCATATTTGA+TGG - chr2_2:61354218-61354237 None:intergenic 20.0%
!! TATATTTCTTTAACATGATC+TGG - chr2_2:61354689-61354708 None:intergenic 20.0%
!! ATAAAACCATATATACATGT+AGG + chr2_2:61354721-61354740 Msa0262420:intron 20.0%
!!! TAGGAGTTATTTTTTGTTTA+GGG + chr2_2:61354740-61354759 Msa0262420:intron 20.0%
!! GATTCTAAGTAAAACTAAAT+TGG - chr2_2:61355099-61355118 None:intergenic 20.0%
!!! TTTAGTTTTACTTAGAATCA+TGG + chr2_2:61355100-61355119 Msa0262420:intron 20.0%
!! TTGTATGTCTAATATGTAAA+AGG + chr2_2:61355397-61355416 Msa0262420:intron 20.0%
!! TGTATGTCTAATATGTAAAA+GGG + chr2_2:61355398-61355417 Msa0262420:intron 20.0%
!!! ATGAAATTGGTTTATGAAAT+TGG + chr2_2:61355466-61355485 Msa0262420:intron 20.0%
!! TTGTGAATCATTACAAATTT+CGG - chr2_2:61355826-61355845 None:intergenic 20.0%
!!! TTTGCTTGTATTTTAGGATT+TGG + chr2_2:61353940-61353959 Msa0262420:intron 25.0%
!! TGGAGGATTTTATTATTATG+TGG + chr2_2:61353960-61353979 Msa0262420:intron 25.0%
!!! ATTTGTGTATCTTTTGAGTT+TGG + chr2_2:61353986-61354005 Msa0262420:intron 25.0%
!!! CACTTATATAGTATTTTAGG+TGG + chr2_2:61354026-61354045 Msa0262420:intron 25.0%
! GATAAATAAGTATTCGTGAA+AGG + chr2_2:61354065-61354084 Msa0262420:intron 25.0%
! GTTCTAATAACTATGATATG+AGG + chr2_2:61354373-61354392 Msa0262420:intron 25.0%
!! TAATTTTATTCCCAACCTTA+TGG - chr2_2:61354475-61354494 None:intergenic 25.0%
!! TTATTTGATGACAAAACAGT+TGG - chr2_2:61354610-61354629 None:intergenic 25.0%
! AGAAATCTCTTGTACAAAAA+TGG + chr2_2:61354641-61354660 Msa0262420:intron 25.0%
! ATAACTCCTACATGTATATA+TGG - chr2_2:61354730-61354749 None:intergenic 25.0%
!!! GTAGGAGTTATTTTTTGTTT+AGG + chr2_2:61354739-61354758 Msa0262420:intron 25.0%
!!! AGGAGTTATTTTTTGTTTAG+GGG + chr2_2:61354741-61354760 Msa0262420:intron 25.0%
!!! ATAATTTTAAATGGTTTGCC+CGG + chr2_2:61355275-61355294 Msa0262420:intron 25.0%
! TGAAGAGTTTAACATCATAT+GGG - chr2_2:61355370-61355389 None:intergenic 25.0%
! ATGAAGAGTTTAACATCATA+TGG - chr2_2:61355371-61355390 None:intergenic 25.0%
! CTTAAATACGTGAATGAAAT+TGG + chr2_2:61355453-61355472 Msa0262420:intron 25.0%
! CTTAAATACGTGAATGAAAT+TGG + chr2_2:61355453-61355472 Msa0262420:intron 25.0%
!! AATAGGTTAATTCTTTTCAG+AGG + chr2_2:61355621-61355640 Msa0262420:intron 25.0%
! TAATGATTCACAAGATGATT+TGG + chr2_2:61355833-61355852 Msa0262420:CDS 25.0%
AAATGCAAGAAGAAGAAAGA+AGG - chr2_2:61353041-61353060 None:intergenic 30.0%
AAGAAGAAGAAGAAAGAGAA+GGG - chr2_2:61353232-61353251 None:intergenic 30.0%
!! ACAAATTTACCACTTTTCAC+CGG - chr2_2:61353548-61353567 None:intergenic 30.0%
!! TTGGGATTTGCTTGTATTTT+AGG + chr2_2:61353934-61353953 Msa0262420:intron 30.0%
!! TGTTTCATGAGAGCATTTAA+AGG + chr2_2:61354128-61354147 Msa0262420:intron 30.0%
!! GTTTCATGAGAGCATTTAAA+GGG + chr2_2:61354129-61354148 Msa0262420:intron 30.0%
!!! GAGCATTTAAAGGGTATTTT+TGG + chr2_2:61354138-61354157 Msa0262420:intron 30.0%
!! TTGCTTATATTTGAGTTGCA+AGG + chr2_2:61354235-61354254 Msa0262420:intron 30.0%
!! TGCTTATATTTGAGTTGCAA+GGG + chr2_2:61354236-61354255 Msa0262420:intron 30.0%
TAACTCCTTAGATATCCATA+AGG + chr2_2:61354457-61354476 Msa0262420:intron 30.0%
CATAGTACTTCTTAAGAAGA+TGG + chr2_2:61354558-61354577 Msa0262420:intron 30.0%
! GTTTTTATTGAGTGCATGTT+TGG + chr2_2:61354789-61354808 Msa0262420:intron 30.0%
ATTTGGTAAAAGCTACACTT+TGG + chr2_2:61354854-61354873 Msa0262420:intron 30.0%
ACAGCTACACATGAAAATTA+CGG - chr2_2:61354903-61354922 None:intergenic 30.0%
!! TTTGGTTTGACAGTAAAGTT+TGG - chr2_2:61354933-61354952 None:intergenic 30.0%
!! TAATTTTAAATGGTTTGCCC+GGG + chr2_2:61355276-61355295 Msa0262420:intron 30.0%
TCTGCTGTTGAAATAAGATA+TGG + chr2_2:61355545-61355564 Msa0262420:intron 30.0%
AAAATGTGTTTGTGCAGAAT+AGG + chr2_2:61355604-61355623 Msa0262420:intron 30.0%
! TGACATCAATTTTCACAAGA+TGG + chr2_2:61355730-61355749 Msa0262420:CDS 30.0%
GAGAGAGAGAGAAATGAAAA+TGG - chr2_2:61353088-61353107 None:intergenic 35.0%
! TCTGCAAACAAACTGGTTTA+TGG + chr2_2:61353112-61353131 Msa0262420:five_prime_UTR 35.0%
GTAGATGTGGTTGAAGTTAA+TGG - chr2_2:61353159-61353178 None:intergenic 35.0%
GAAGAAGAAGAAGAAAGAGA+AGG - chr2_2:61353233-61353252 None:intergenic 35.0%
CATGATCATCCACTAGAATT+GGG - chr2_2:61353698-61353717 None:intergenic 35.0%
TCATGATCATCCACTAGAAT+TGG - chr2_2:61353699-61353718 None:intergenic 35.0%
!!! GCTTGTATTTTAGGATTTGG+AGG + chr2_2:61353943-61353962 Msa0262420:intron 35.0%
! CTTTGTGGATTATGTATGCT+AGG + chr2_2:61354291-61354310 Msa0262420:intron 35.0%
TCCTTAGATATCCATAAGGT+TGG + chr2_2:61354461-61354480 Msa0262420:intron 35.0%
CCTTAGATATCCATAAGGTT+GGG + chr2_2:61354462-61354481 Msa0262420:intron 35.0%
TTTGTTTAGGGGATACAAGT+AGG + chr2_2:61354752-61354771 Msa0262420:intron 35.0%
! TAGCTTTTACCAAATCACAG+TGG - chr2_2:61354849-61354868 None:intergenic 35.0%
!! AAACAAGGGTGATGTTTTTG+TGG + chr2_2:61354967-61354986 Msa0262420:intron 35.0%
!! AACAAGGGTGATGTTTTTGT+GGG + chr2_2:61354968-61354987 Msa0262420:intron 35.0%
!!! AGTTTTTTTGTGCCCTTTTG+AGG + chr2_2:61355007-61355026 Msa0262420:intron 35.0%
TTAGTAACTCTGCCTCAAAA+GGG - chr2_2:61355022-61355041 None:intergenic 35.0%
ATTAGTAACTCTGCCTCAAA+AGG - chr2_2:61355023-61355042 None:intergenic 35.0%
!!! TGTCTTTGTTGTTTTGTGCT+TGG + chr2_2:61355055-61355074 Msa0262420:intron 35.0%
TTCACGTATTTAAGCCACAA+TGG - chr2_2:61355447-61355466 None:intergenic 35.0%
GTTTATGAAATTGGCCATTG+TGG + chr2_2:61355475-61355494 Msa0262420:intron 35.0%
TTCACGTATTTAAGCCACAA+TGG - chr2_2:61355447-61355466 None:intergenic 35.0%
CAGATGTAACATAGCATGAT+AGG - chr2_2:61355529-61355548 None:intergenic 35.0%
AAATTGATGTCAGTTCCAGA+GGG - chr2_2:61355722-61355741 None:intergenic 35.0%
AAAATTGATGTCAGTTCCAG+AGG - chr2_2:61355723-61355742 None:intergenic 35.0%
GCAAGAAGAAGAAAGAAGGA+AGG - chr2_2:61353037-61353056 None:intergenic 40.0%
!! TGGGTTTGTTATCGTAGATG+TGG - chr2_2:61353172-61353191 None:intergenic 40.0%
!!! GTTTTGGTGGTGGTTGTTAT+GGG - chr2_2:61353191-61353210 None:intergenic 40.0%
!!! CTTTGAAGCGTTGTTGGTTT+TGG - chr2_2:61353207-61353226 None:intergenic 40.0%
! GTTGGATGTGAAATGGGTTT+GGG - chr2_2:61353338-61353357 None:intergenic 40.0%
! CTAGTGGATGATCATGATCA+TGG + chr2_2:61353702-61353721 Msa0262420:exon 40.0%
AGGAGGATTAGATTGCTTTG+TGG + chr2_2:61354276-61354295 Msa0262420:intron 40.0%
CCCAACCTTATGGATATCTA+AGG - chr2_2:61354465-61354484 None:intergenic 40.0%
! TGAGTGCATGTTTGGATTAG+CGG + chr2_2:61354797-61354816 Msa0262420:intron 40.0%
! TTTGGAGCTACCCAAAATCA+TGG + chr2_2:61354872-61354891 Msa0262420:intron 40.0%
CTGTCAAACCAAACATACAC+CGG + chr2_2:61354940-61354959 Msa0262420:intron 40.0%
!! GTTTTGATCCGGTGTATGTT+TGG - chr2_2:61354951-61354970 None:intergenic 40.0%
ACATACACCGGATCAAAACA+AGG + chr2_2:61354952-61354971 Msa0262420:intron 40.0%
CATACACCGGATCAAAACAA+GGG + chr2_2:61354953-61354972 Msa0262420:intron 40.0%
!!! ACATCACCCTTGTTTTGATC+CGG - chr2_2:61354962-61354981 None:intergenic 40.0%
!! ACAAGGGTGATGTTTTTGTG+GGG + chr2_2:61354969-61354988 Msa0262420:intron 40.0%
TTGTGGGGTTCTTGATTAAG+AGG + chr2_2:61354984-61355003 Msa0262420:intron 40.0%
TTGAGGCAGAGTTACTAATG+TGG + chr2_2:61355024-61355043 Msa0262420:intron 40.0%
TGAGGCAGAGTTACTAATGT+GGG + chr2_2:61355025-61355044 Msa0262420:intron 40.0%
TTTGGATGAAGGGTGAAATG+AGG - chr2_2:61355157-61355176 None:intergenic 40.0%
CGGTTACAAATTGCGATGTT+AGG - chr2_2:61355806-61355825 None:intergenic 40.0%
CTCTCTCTCTGCAAACAAAC+TGG + chr2_2:61353105-61353124 Msa0262420:five_prime_UTR 45.0%
!!! GGTTTTGGTGGTGGTTGTTA+TGG - chr2_2:61353192-61353211 None:intergenic 45.0%
!!! TGAAGCGTTGTTGGTTTTGG+TGG - chr2_2:61353204-61353223 None:intergenic 45.0%
!! AGGGTTCTTTGAAGCGTTGT+TGG - chr2_2:61353213-61353232 None:intergenic 45.0%
GGAAACTGCAGAATACGAAG+CGG - chr2_2:61353270-61353289 None:intergenic 45.0%
GTTTCCGCACCAAACAAACA+TGG + chr2_2:61353284-61353303 Msa0262420:five_prime_UTR 45.0%
AGGATTCGGCCATGTTTGTT+TGG - chr2_2:61353296-61353315 None:intergenic 45.0%
GTCGGGTTAAAACGAGGATT+CGG - chr2_2:61353310-61353329 None:intergenic 45.0%
! TGTGAAATGGGTTTGGGTGT+GGG - chr2_2:61353332-61353351 None:intergenic 45.0%
! ATGTGAAATGGGTTTGGGTG+TGG - chr2_2:61353333-61353352 None:intergenic 45.0%
! GGTTGGATGTGAAATGGGTT+TGG - chr2_2:61353339-61353358 None:intergenic 45.0%
! TGTTGTTGTTGGATCGGGTT+GGG - chr2_2:61353386-61353405 None:intergenic 45.0%
AAACTGGAGGCTGTTGTTGT+TGG - chr2_2:61353397-61353416 None:intergenic 45.0%
!! AAAGAACCGATTCGACCCAA+CGG - chr2_2:61353470-61353489 None:intergenic 45.0%
! TCGAATCGGTTCTTTGTCGT+CGG + chr2_2:61353475-61353494 Msa0262420:five_prime_UTR 45.0%
!!! TTACCGAACCCGGTTTTGAA+CGG + chr2_2:61353503-61353522 Msa0262420:five_prime_UTR 45.0%
TCTTTCTCGCCGTTCAAAAC+CGG - chr2_2:61353515-61353534 None:intergenic 45.0%
CGAGAAAGAGAAGAGTTTGC+CGG + chr2_2:61353526-61353545 Msa0262420:five_prime_UTR 45.0%
AAGAGTTTGCCGGTGAAAAG+TGG + chr2_2:61353536-61353555 Msa0262420:five_prime_UTR 45.0%
AGAGGATGAGGAAGGTTTCT+GGG + chr2_2:61353648-61353667 Msa0262420:five_prime_UTR 45.0%
! GAAGGTTTCTGGGTCTGATA+CGG + chr2_2:61353658-61353677 Msa0262420:five_prime_UTR 45.0%
! AAGGTTTCTGGGTCTGATAC+GGG + chr2_2:61353659-61353678 Msa0262420:five_prime_UTR 45.0%
!! GAGTTGCAAGGGAGTATCTT+AGG + chr2_2:61354247-61354266 Msa0262420:intron 45.0%
!! CAAGGGAGTATCTTAGGACT+TGG + chr2_2:61354253-61354272 Msa0262420:intron 45.0%
AGTATCTTAGGACTTGGAGG+AGG + chr2_2:61354259-61354278 Msa0262420:intron 45.0%
! TACGGCGACACCATGATTTT+GGG - chr2_2:61354885-61354904 None:intergenic 45.0%
! TTACGGCGACACCATGATTT+TGG - chr2_2:61354886-61354905 None:intergenic 45.0%
CAACCAGATGCACAAAGCTT+TGG + chr2_2:61355421-61355440 Msa0262420:intron 45.0%
GTACATATCCTTTGTCCCTC+TGG + chr2_2:61355704-61355723 Msa0262420:CDS 45.0%
! GGTGTTGAGAATCTGTCTTC+TGG - chr2_2:61355785-61355804 None:intergenic 45.0%
!!! TCATTTTTTTTTTTTTTTTT+TGG - chr2_2:61355175-61355194 None:intergenic 5.0%
!! AAAAAAAAAAAAAAAATGAA+TGG + chr2_2:61355176-61355195 Msa0262420:intron 5.0%
!!! CTTTATTTAATAATTTTAAA+TGG + chr2_2:61355266-61355285 Msa0262420:intron 5.0%
!!! AGCGTTGTTGGTTTTGGTGG+TGG - chr2_2:61353201-61353220 None:intergenic 50.0%
AGAATACGAAGCGGAGGAGA+AGG - chr2_2:61353261-61353280 None:intergenic 50.0%
AACTGCAGAATACGAAGCGG+AGG - chr2_2:61353267-61353286 None:intergenic 50.0%
! TCGGCCATGTTTGTTTGGTG+CGG - chr2_2:61353291-61353310 None:intergenic 50.0%
! AAATGGGTTTGGGTGTGGGT+CGG - chr2_2:61353328-61353347 None:intergenic 50.0%
GGTGGGGTTGGATGTGAAAT+GGG - chr2_2:61353344-61353363 None:intergenic 50.0%
!! TGTTGGATCGGGTTGGGTTT+GGG - chr2_2:61353380-61353399 None:intergenic 50.0%
!! TTGTTGGATCGGGTTGGGTT+TGG - chr2_2:61353381-61353400 None:intergenic 50.0%
! CTGTTGTTGTTGGATCGGGT+TGG - chr2_2:61353387-61353406 None:intergenic 50.0%
! GAGGCTGTTGTTGTTGGATC+GGG - chr2_2:61353391-61353410 None:intergenic 50.0%
! GGAGGCTGTTGTTGTTGGAT+CGG - chr2_2:61353392-61353411 None:intergenic 50.0%
GGTCGATCTGAAGAAACTGG+AGG - chr2_2:61353410-61353429 None:intergenic 50.0%
CAGTTTCTTCAGATCGACCC+CGG + chr2_2:61353411-61353430 Msa0262420:five_prime_UTR 50.0%
AGCGAGATTGAATCTGGTCC+CGG - chr2_2:61353443-61353462 None:intergenic 50.0%
GGACGAAGCGAGATTGAATC+TGG - chr2_2:61353449-61353468 None:intergenic 50.0%
TTCAATCTCGCTTCGTCCGT+TGG + chr2_2:61353451-61353470 Msa0262420:five_prime_UTR 50.0%
TCAATCTCGCTTCGTCCGTT+GGG + chr2_2:61353452-61353471 Msa0262420:five_prime_UTR 50.0%
! CGAATCGGTTCTTTGTCGTC+GGG + chr2_2:61353476-61353495 Msa0262420:five_prime_UTR 50.0%
CTTTCTCGCCGTTCAAAACC+GGG - chr2_2:61353514-61353533 None:intergenic 50.0%
GAAAACTTGCGACCCAAGTC+TGG + chr2_2:61353617-61353636 Msa0262420:five_prime_UTR 50.0%
CTCTCATTCCCACCAGACTT+GGG - chr2_2:61353632-61353651 None:intergenic 50.0%
TGGGAATGAGAGGATGAGGA+AGG + chr2_2:61353640-61353659 Msa0262420:five_prime_UTR 50.0%
GAGAGGATGAGGAAGGTTTC+TGG + chr2_2:61353647-61353666 Msa0262420:five_prime_UTR 50.0%
! TCTGGGTCTGATACGGGTTT+TGG + chr2_2:61353665-61353684 Msa0262420:five_prime_UTR 50.0%
! CTGGGTCTGATACGGGTTTT+GGG + chr2_2:61353666-61353685 Msa0262420:five_prime_UTR 50.0%
GGGAATCGACCCAATTCTAG+TGG + chr2_2:61353686-61353705 Msa0262420:five_prime_UTR 50.0%
!! GGGAGTATCTTAGGACTTGG+AGG + chr2_2:61354256-61354275 Msa0262420:intron 50.0%
!! GCATGTTTGGATTAGCGGTG+AGG + chr2_2:61354802-61354821 Msa0262420:intron 50.0%
CTTGGTGAGCCACTGTGATT+TGG + chr2_2:61354837-61354856 Msa0262420:intron 50.0%
GACTCAAACCCGGAACTAGT+CGG + chr2_2:61355298-61355317 Msa0262420:intron 50.0%
! TACTCCATCCGACTAGTTCC+GGG - chr2_2:61355309-61355328 None:intergenic 50.0%
! CTACTCCATCCGACTAGTTC+CGG - chr2_2:61355310-61355329 None:intergenic 50.0%
! TGGCCAAAGCTTTGTGCATC+TGG - chr2_2:61355427-61355446 None:intergenic 50.0%
! GCACAAAGCTTTGGCCATTG+TGG + chr2_2:61355430-61355449 Msa0262420:intron 50.0%
TGTCAGTTCCAGAGGGACAA+AGG - chr2_2:61355715-61355734 None:intergenic 50.0%
GTGTGGGTCGGGTTAAAACG+AGG - chr2_2:61353316-61353335 None:intergenic 55.0%
! AATGGGTTTGGGTGTGGGTC+GGG - chr2_2:61353327-61353346 None:intergenic 55.0%
GGGTGGGGTTGGATGTGAAA+TGG - chr2_2:61353345-61353364 None:intergenic 55.0%
GGTTTGGGCTGTGATTGTGC+GGG - chr2_2:61353365-61353384 None:intergenic 55.0%
GGGTTTGGGCTGTGATTGTG+CGG - chr2_2:61353366-61353385 None:intergenic 55.0%
CGGGGTCGATCTGAAGAAAC+TGG - chr2_2:61353413-61353432 None:intergenic 55.0%
AATCTGGTCCCGGTTCAGAC+CGG - chr2_2:61353433-61353452 None:intergenic 55.0%
CTTCGTCCGTTGGGTCGAAT+CGG + chr2_2:61353461-61353480 Msa0262420:five_prime_UTR 55.0%
! GTCGGGTCTTTTACCGAACC+CGG + chr2_2:61353493-61353512 Msa0262420:five_prime_UTR 55.0%
TCGCCGTTCAAAACCGGGTT+CGG - chr2_2:61353509-61353528 None:intergenic 55.0%
ACAAACCCCGGACGAAGATG+CGG - chr2_2:61353575-61353594 None:intergenic 55.0%
TCCCTAAGACACTCCTCCCT+CGG - chr2_2:61353599-61353618 None:intergenic 55.0%
AACTTGCGACCCAAGTCTGG+TGG + chr2_2:61353620-61353639 Msa0262420:five_prime_UTR 55.0%
ACTTGCGACCCAAGTCTGGT+GGG + chr2_2:61353621-61353640 Msa0262420:five_prime_UTR 55.0%
CCTCTCATTCCCACCAGACT+TGG - chr2_2:61353633-61353652 None:intergenic 55.0%
CCAAGTCTGGTGGGAATGAG+AGG + chr2_2:61353630-61353649 Msa0262420:five_prime_UTR 55.0%
CTGGTGGGAATGAGAGGATG+AGG + chr2_2:61353636-61353655 Msa0262420:five_prime_UTR 55.0%
GTGAGGTTGCCATGCTAGCT+TGG + chr2_2:61354819-61354838 Msa0262420:intron 55.0%
AGTGGCTCACCAAGCTAGCA+TGG - chr2_2:61354831-61354850 None:intergenic 55.0%
TAGTTCCGGGTTTGAGTCCC+GGG - chr2_2:61355296-61355315 None:intergenic 55.0%
CTAGTTCCGGGTTTGAGTCC+CGG - chr2_2:61355297-61355316 None:intergenic 55.0%
CAAACCCGGAACTAGTCGGA+TGG + chr2_2:61355302-61355321 Msa0262420:intron 55.0%
TGTGATTGTGCGGGTGGGGT+TGG - chr2_2:61353356-61353375 None:intergenic 60.0%
TGGGCTGTGATTGTGCGGGT+GGG - chr2_2:61353361-61353380 None:intergenic 60.0%
TTGGGCTGTGATTGTGCGGG+TGG - chr2_2:61353362-61353381 None:intergenic 60.0%
AGATCGACCCCGGTCTGAAC+CGG + chr2_2:61353421-61353440 Msa0262420:five_prime_UTR 60.0%
ATCTGGTCCCGGTTCAGACC+GGG - chr2_2:61353432-61353451 None:intergenic 60.0%
!! TGCCGAGGGAGGAGTGTCTT+AGG + chr2_2:61353594-61353613 Msa0262420:five_prime_UTR 60.0%
!! GCCGAGGGAGGAGTGTCTTA+GGG + chr2_2:61353595-61353614 Msa0262420:five_prime_UTR 60.0%
GTTTGCCCGGGACTCAAACC+CGG + chr2_2:61355288-61355307 Msa0262420:intron 60.0%
GGGCTGTGATTGTGCGGGTG+GGG - chr2_2:61353360-61353379 None:intergenic 65.0%
GATCGACCCCGGTCTGAACC+GGG + chr2_2:61353422-61353441 Msa0262420:five_prime_UTR 65.0%
TCTGGTCCCGGTTCAGACCG+GGG - chr2_2:61353431-61353450 None:intergenic 65.0%
TGTGCCGCCGCATCTTCGTC+CGG + chr2_2:61353565-61353584 Msa0262420:five_prime_UTR 65.0%
AACCCCGGACGAAGATGCGG+CGG - chr2_2:61353572-61353591 None:intergenic 65.0%
TTCGTCCGGGGTTTGTGCCG+AGG + chr2_2:61353579-61353598 Msa0262420:five_prime_UTR 65.0%
TCGTCCGGGGTTTGTGCCGA+GGG + chr2_2:61353580-61353599 Msa0262420:five_prime_UTR 65.0%
TCCTCCCTCGGCACAAACCC+CGG - chr2_2:61353587-61353606 None:intergenic 65.0%
GTGCCGCCGCATCTTCGTCC+GGG + chr2_2:61353566-61353585 Msa0262420:five_prime_UTR 70.0%
TGCCGCCGCATCTTCGTCCG+GGG + chr2_2:61353567-61353586 Msa0262420:five_prime_UTR 70.0%
TCCGGGGTTTGTGCCGAGGG+AGG + chr2_2:61353583-61353602 Msa0262420:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 61353022 61355872 61353022 ID=Msa0262420;Name=Msa0262420
chr2_2 mRNA 61353022 61355872 61353022 ID=Msa0262420-mRNA-1;Parent=Msa0262420;Name=Msa0262420-mRNA-1;_AED=0.11;_eAED=0.15;_QI=687|0|0.5|1|0|0|2|0|64
chr2_2 exon 61353022 61353723 61353022 ID=Msa0262420-mRNA-1:exon:18872;Parent=Msa0262420-mRNA-1
chr2_2 exon 61355693 61355872 61355693 ID=Msa0262420-mRNA-1:exon:18873;Parent=Msa0262420-mRNA-1
chr2_2 five_prime_UTR 61353022 61353708 61353022 ID=Msa0262420-mRNA-1:five_prime_utr;Parent=Msa0262420-mRNA-1
chr2_2 CDS 61353709 61353723 61353709 ID=Msa0262420-mRNA-1:cds;Parent=Msa0262420-mRNA-1
chr2_2 CDS 61355693 61355872 61355693 ID=Msa0262420-mRNA-1:cds;Parent=Msa0262420-mRNA-1
Gene Sequence

>Msa0262420

ATGATCATGATCATGAACAGTTCTCTGTACATATCCTTTGTCCCTCTGGAACTGACATCAATTTTCACAAGATGGCATCTGAAATCATTGAAGATGAAACATGACCAGAAGACAGATTCTCAACACCTAACATCGCAATTTGTAACCGAAATTTGTAATGATTCACAAGATGATTTGGTTCTTGTTTACCACTGA
Protein sequence

>Msa0262420

MIMIMNSSLYISFVPLELTSIFTRWHLKSLKMKHDQKTDSQHLTSQFVTEICNDSQDDLVLVYH*