AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0220970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220970 MtrunA17_Chr2g0325041 81.633 49 9 0 4 52 3 51 7.38e-23 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGGTCTGATACGGGTTT+TGG 0.179646 2_1:+69503490 Msa0220970:five_prime_UTR
TCTGCAAACAAACTGGTTTA+TGG 0.217646 2_1:+69502937 Msa0220970:five_prime_UTR
GAGAGGATGAGGAAGGTTTC+TGG 0.226601 2_1:+69503472 Msa0220970:five_prime_UTR
TGCCGAGGGAGGAGTGTCTT+AGG 0.240149 2_1:+69503419 Msa0220970:five_prime_UTR
CTTTGAAGCGTTGTTGGTTT+TGG 0.257075 2_1:-69503029 None:intergenic
TCTTTCTCGCCGTTCAAAAC+CGG 0.266026 2_1:-69503337 None:intergenic
TTGTGAATCATTACAAATTT+CGG 0.267761 2_1:-69505612 None:intergenic
TGTTGGATCGGGTTGGGTTT+GGG 0.289585 2_1:-69503202 None:intergenic
GTAGATGTGGTTGAAGTTAA+TGG 0.296091 2_1:-69502981 None:intergenic
TTGTTGGATCGGGTTGGGTT+TGG 0.297089 2_1:-69503203 None:intergenic
TCATAATCATCCACTAGAAT+TGG 0.305471 2_1:-69503521 None:intergenic
TAATGATTCACAAGATGATT+TGG 0.306096 2_1:+69505622 Msa0220970:CDS
ATGTGAAATGGGTTTGGGTG+TGG 0.311571 2_1:-69503155 None:intergenic
TTGGGCTGTGATTGTGCGGG+TGG 0.336985 2_1:-69503184 None:intergenic
GGGTGGGGTTGGATGTGAAA+TGG 0.337055 2_1:-69503167 None:intergenic
CGGTTACAAATTGCGATGTT+AGG 0.340431 2_1:-69505592 None:intergenic
GGTTGGATGTGAAATGGGTT+TGG 0.349429 2_1:-69503161 None:intergenic
GGTGGGGTTGGATGTGAAAT+GGG 0.353408 2_1:-69503166 None:intergenic
TTCAATCTCGCTTCGTCCGT+TGG 0.354260 2_1:+69503276 Msa0220970:five_prime_UTR
CTTTCTCGCCGTTCAAAACC+GGG 0.355045 2_1:-69503336 None:intergenic
TCGCCGTTCAAAACCGGGTT+CGG 0.362760 2_1:-69503331 None:intergenic
GGTGTTGAGAATCTGTCTTC+TGG 0.363513 2_1:-69505571 None:intergenic
CCTCTCATTCCCACCAGACT+TGG 0.365997 2_1:-69503455 None:intergenic
AATGGGTTTGGGTGTGGGTC+GGG 0.366489 2_1:-69503149 None:intergenic
GTTGGATGTGAAATGGGTTT+GGG 0.371160 2_1:-69503160 None:intergenic
TGTGCCGCCGCATCTTCGTC+CGG 0.384242 2_1:+69503390 Msa0220970:five_prime_UTR
AGGATTCGGCCATGTTTGTT+TGG 0.385651 2_1:-69503118 None:intergenic
AAATGGGTTTGGGTGTGGGT+CGG 0.388508 2_1:-69503150 None:intergenic
AGATCGACCCCGGTCTGAAC+CGG 0.391141 2_1:+69503246 Msa0220970:five_prime_UTR
GTACATATCCTTTGTCCCTC+TGG 0.393138 2_1:+69505493 Msa0220970:CDS
TCAATCTCGCTTCGTCCGTT+GGG 0.395438 2_1:+69503277 Msa0220970:five_prime_UTR
TGTGATTGTGCGGGTGGGGT+TGG 0.395841 2_1:-69503178 None:intergenic
CTCTCTCTCTGCAAACAAAC+TGG 0.399039 2_1:+69502930 Msa0220970:five_prime_UTR
TGGGCTGTGATTGTGCGGGT+GGG 0.405665 2_1:-69503183 None:intergenic
TGTTGTTGTTGGATCGGGTT+GGG 0.408930 2_1:-69503208 None:intergenic
GAAGGTTTCTGGGTCTGATA+CGG 0.416702 2_1:+69503483 Msa0220970:five_prime_UTR
CGGGGTCGATCTGAAGAAAC+TGG 0.419897 2_1:-69503235 None:intergenic
GGACGAAGCGAGATTGAATC+TGG 0.420282 2_1:-69503271 None:intergenic
TGTGAAATGGGTTTGGGTGT+GGG 0.421896 2_1:-69503154 None:intergenic
GTGCCGCCGCATCTTCGTCC+GGG 0.426862 2_1:+69503391 Msa0220970:five_prime_UTR
TCGAATCGGTTCTTTGTCGT+CGG 0.435485 2_1:+69503300 Msa0220970:five_prime_UTR
AGGGTTCTTTGAAGCGTTGT+TGG 0.436197 2_1:-69503035 None:intergenic
GGGCTGTGATTGTGCGGGTG+GGG 0.445432 2_1:-69503182 None:intergenic
TCGTCCGGGGTTTGTGCCGA+GGG 0.450244 2_1:+69503405 Msa0220970:five_prime_UTR
ATCTGGTCCCGGTTCAGACC+GGG 0.453477 2_1:-69503254 None:intergenic
GAAAACTTGCGACCCAAGTC+TGG 0.455303 2_1:+69503442 Msa0220970:five_prime_UTR
AAACTGGAGGCTGTTGTTGT+TGG 0.455936 2_1:-69503219 None:intergenic
AGAGGATGAGGAAGGTTTCT+GGG 0.456615 2_1:+69503473 Msa0220970:five_prime_UTR
CTGTTGTTGTTGGATCGGGT+TGG 0.464894 2_1:-69503209 None:intergenic
CATAATCATCCACTAGAATT+GGG 0.469664 2_1:-69503520 None:intergenic
CTTCGTCCGTTGGGTCGAAT+CGG 0.470054 2_1:+69503286 Msa0220970:five_prime_UTR
CGAATCGGTTCTTTGTCGTC+GGG 0.470387 2_1:+69503301 Msa0220970:five_prime_UTR
CTGGTGGGAATGAGAGGATG+AGG 0.472518 2_1:+69503461 Msa0220970:five_prime_UTR
GGGTTTGGGCTGTGATTGTG+CGG 0.473346 2_1:-69503188 None:intergenic
CTAGTGGATGATTATGATCA+TGG 0.476961 2_1:+69503527 Msa0220970:exon
TCCCTAAGACACTCCTCCCT+CGG 0.483826 2_1:-69503421 None:intergenic
CTCTCATTCCCACCAGACTT+GGG 0.484303 2_1:-69503454 None:intergenic
GAGGCTGTTGTTGTTGGATC+GGG 0.484796 2_1:-69503213 None:intergenic
CGAGAAAGAGAAGAGTTTGC+CGG 0.486001 2_1:+69503351 Msa0220970:five_prime_UTR
TCCTCCCTCGGCACAAACCC+CGG 0.493049 2_1:-69503409 None:intergenic
AAGGTTTCTGGGTCTGATAC+GGG 0.495133 2_1:+69503484 Msa0220970:five_prime_UTR
AAGAAGAAGAAGAAAGAGAA+GGG 0.496336 2_1:-69503054 None:intergenic
GCAAGAAGAAGAAAGAAGGA+AGG 0.498038 2_1:-69502863 None:intergenic
TTCGTCCGGGGTTTGTGCCG+AGG 0.499430 2_1:+69503404 Msa0220970:five_prime_UTR
GGAGGCTGTTGTTGTTGGAT+CGG 0.500195 2_1:-69503214 None:intergenic
GTCGGGTTAAAACGAGGATT+CGG 0.502204 2_1:-69503132 None:intergenic
AGAATACGAAGCGGAGGAGA+AGG 0.505453 2_1:-69503083 None:intergenic
GCCGAGGGAGGAGTGTCTTA+GGG 0.509355 2_1:+69503420 Msa0220970:five_prime_UTR
AAATGCAAGAAGAAGAAAGA+AGG 0.510872 2_1:-69502867 None:intergenic
AATCTGGTCCCGGTTCAGAC+CGG 0.519367 2_1:-69503255 None:intergenic
AGCGAGATTGAATCTGGTCC+CGG 0.526016 2_1:-69503265 None:intergenic
GATCGACCCCGGTCTGAACC+GGG 0.543684 2_1:+69503247 Msa0220970:five_prime_UTR
CCAAGTCTGGTGGGAATGAG+AGG 0.547204 2_1:+69503455 Msa0220970:five_prime_UTR
GGGAATCGACCCAATTCTAG+TGG 0.555420 2_1:+69503511 Msa0220970:five_prime_UTR
AACTTGCGACCCAAGTCTGG+TGG 0.558391 2_1:+69503445 Msa0220970:five_prime_UTR
GGTTTGGGCTGTGATTGTGC+GGG 0.560295 2_1:-69503187 None:intergenic
TGCCGCCGCATCTTCGTCCG+GGG 0.561537 2_1:+69503392 Msa0220970:five_prime_UTR
TCCGGGGTTTGTGCCGAGGG+AGG 0.569379 2_1:+69503408 Msa0220970:five_prime_UTR
TGGGAATGAGAGGATGAGGA+AGG 0.570759 2_1:+69503465 Msa0220970:five_prime_UTR
CAGTTTCTTCAGATCGACCC+CGG 0.573018 2_1:+69503236 Msa0220970:five_prime_UTR
AAGAGTTTGCCGGTGAAAAG+TGG 0.574187 2_1:+69503361 Msa0220970:five_prime_UTR
TGGGTTTGTTATCGTAGATG+TGG 0.577249 2_1:-69502994 None:intergenic
TCGGCCATGTTTGTTTGGTG+CGG 0.579784 2_1:-69503113 None:intergenic
ACAAACCCCGGACGAAGATG+CGG 0.590708 2_1:-69503397 None:intergenic
TCTGGTCCCGGTTCAGACCG+GGG 0.604345 2_1:-69503253 None:intergenic
AAAATTGATGTCAGTTCCAG+AGG 0.606435 2_1:-69505509 None:intergenic
GTGTGGGTCGGGTTAAAACG+AGG 0.611653 2_1:-69503138 None:intergenic
TGTCAGTTCCAGAGGGACAA+AGG 0.616152 2_1:-69505501 None:intergenic
ACTTGCGACCCAAGTCTGGT+GGG 0.619752 2_1:+69503446 Msa0220970:five_prime_UTR
AACCCCGGACGAAGATGCGG+CGG 0.626124 2_1:-69503394 None:intergenic
AACTGCAGAATACGAAGCGG+AGG 0.631412 2_1:-69503089 None:intergenic
GTTTCCGCACCAAACAAACA+TGG 0.648595 2_1:+69503109 Msa0220970:five_prime_UTR
AAAGAACCGATTCGACCCAA+CGG 0.649372 2_1:-69503292 None:intergenic
AAATTGATGTCAGTTCCAGA+GGG 0.658418 2_1:-69505508 None:intergenic
GGTCGATCTGAAGAAACTGG+AGG 0.681748 2_1:-69503232 None:intergenic
GGAAACTGCAGAATACGAAG+CGG 0.759361 2_1:-69503092 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATATGATGAAATTAAT+TGG + chr2_1:69503740-69503759 Msa0220970:intron 10.0%
!! TAAATATGATGAAATTAATT+GGG + chr2_1:69503741-69503760 Msa0220970:intron 10.0%
!! TTTCTAAATTAGTTAACATA+TGG - chr2_1:69503688-69503707 None:intergenic 15.0%
!!! AATCACTTATATAGTATTTT+AGG + chr2_1:69503848-69503867 Msa0220970:intron 15.0%
!! GAATGATAACATTAATTATA+TGG + chr2_1:69503985-69504004 Msa0220970:intron 15.0%
!!! TTTTTTTTTTTTTGGATGAA+GGG - chr2_1:69504959-69504978 None:intergenic 15.0%
!!! TTTTTTTTTTTTTTGGATGA+AGG - chr2_1:69504960-69504979 None:intergenic 15.0%
!!! CATTCAGTTTTTTTTTTTTT+TGG - chr2_1:69504967-69504986 None:intergenic 15.0%
!! CAAAAAAAAAAAAAACTGAA+TGG + chr2_1:69504965-69504984 Msa0220970:intron 15.0%
!!! TTTTCTTGATTGTTCTTTAA+TGG + chr2_1:69503612-69503631 Msa0220970:intron 20.0%
!! CAACAAAATATCATATTTGA+TGG - chr2_1:69504043-69504062 None:intergenic 20.0%
!! TATATTTCTTTAACATGATC+TGG - chr2_1:69504504-69504523 None:intergenic 20.0%
!! ATAAAACCATATATACATGT+AGG + chr2_1:69504536-69504555 Msa0220970:intron 20.0%
!!! TAGGAGTTATTTTTTGTTTA+GGG + chr2_1:69504555-69504574 Msa0220970:intron 20.0%
!! GATTCTAAGTAAAACTAAAT+TGG - chr2_1:69504891-69504910 None:intergenic 20.0%
!!! TTTAGTTTTACTTAGAATCA+TGG + chr2_1:69504892-69504911 Msa0220970:intron 20.0%
!! TTGTATGTCTAATATGTAAA+AGG + chr2_1:69505186-69505205 Msa0220970:intron 20.0%
!! TGTATGTCTAATATGTAAAA+GGG + chr2_1:69505187-69505206 Msa0220970:intron 20.0%
!! TTGTGAATCATTACAAATTT+CGG - chr2_1:69505615-69505634 None:intergenic 20.0%
!!! TTTGCTTGTATTTTAGGATT+TGG + chr2_1:69503765-69503784 Msa0220970:intron 25.0%
!! TGGAGGATTTTATTATTATG+TGG + chr2_1:69503785-69503804 Msa0220970:intron 25.0%
!!! CACTTATATAGTATTTTAGG+TGG + chr2_1:69503851-69503870 Msa0220970:intron 25.0%
! GATAAATAAGTATTCGTGAA+AGG + chr2_1:69503890-69503909 Msa0220970:intron 25.0%
! GTTCTAATAACTATGATATG+AGG + chr2_1:69504198-69504217 Msa0220970:intron 25.0%
!!! TAATTTTTTTCCCAACCTTA+AGG - chr2_1:69504300-69504319 None:intergenic 25.0%
!! TTATTTGATGACAAAACAGT+TGG - chr2_1:69504425-69504444 None:intergenic 25.0%
! AGAAATCTCTTGTACAAAAA+TGG + chr2_1:69504456-69504475 Msa0220970:intron 25.0%
! ATAACTCCTACATGTATATA+TGG - chr2_1:69504545-69504564 None:intergenic 25.0%
!!! GTAGGAGTTATTTTTTGTTT+AGG + chr2_1:69504554-69504573 Msa0220970:intron 25.0%
!!! AGGAGTTATTTTTTGTTTAG+GGG + chr2_1:69504556-69504575 Msa0220970:intron 25.0%
!!! ATAATTTTAAATGGTTTGCC+CGG + chr2_1:69505064-69505083 Msa0220970:intron 25.0%
! TGAAGAGTTTAACATCATAT+GGG - chr2_1:69505159-69505178 None:intergenic 25.0%
! ATGAAGAGTTTAACATCATA+TGG - chr2_1:69505160-69505179 None:intergenic 25.0%
! CTTAAATACGTGAATGAAAT+TGG + chr2_1:69505242-69505261 Msa0220970:intron 25.0%
! CTTAAATACGTGAATGAAAT+TGG + chr2_1:69505242-69505261 Msa0220970:intron 25.0%
!! AATAGGTTAATTCTTTTCAG+AGG + chr2_1:69505410-69505429 Msa0220970:intron 25.0%
! TAATGATTCACAAGATGATT+TGG + chr2_1:69505622-69505641 Msa0220970:CDS 25.0%
AAATGCAAGAAGAAGAAAGA+AGG - chr2_1:69502870-69502889 None:intergenic 30.0%
AAGAAGAAGAAGAAAGAGAA+GGG - chr2_1:69503057-69503076 None:intergenic 30.0%
!! ACAAATTTACCACTTTTCAC+CGG - chr2_1:69503373-69503392 None:intergenic 30.0%
CATAATCATCCACTAGAATT+GGG - chr2_1:69503523-69503542 None:intergenic 30.0%
TCATAATCATCCACTAGAAT+TGG - chr2_1:69503524-69503543 None:intergenic 30.0%
!! TTGGGATTTGCTTGTATTTT+AGG + chr2_1:69503759-69503778 Msa0220970:intron 30.0%
!! TGTTTCATGAGAGCATTTAA+AGG + chr2_1:69503953-69503972 Msa0220970:intron 30.0%
!! GTTTCATGAGAGCATTTAAA+GGG + chr2_1:69503954-69503973 Msa0220970:intron 30.0%
!!! GAGCATTTAAAGGGTATTTT+TGG + chr2_1:69503963-69503982 Msa0220970:intron 30.0%
!! TTGCTTATATTTGAGTTGCA+AGG + chr2_1:69504060-69504079 Msa0220970:intron 30.0%
!! TGCTTATATTTGAGTTGCAA+GGG + chr2_1:69504061-69504080 Msa0220970:intron 30.0%
TAACTCCTTAGATATCCTTA+AGG + chr2_1:69504282-69504301 Msa0220970:intron 30.0%
CATAGTACTTCTTAAGAAGA+TGG + chr2_1:69504373-69504392 Msa0220970:intron 30.0%
! GTTTTTATTGAGTGCATGTT+TGG + chr2_1:69504604-69504623 Msa0220970:intron 30.0%
ATTTGGTAAAAGCTACACTT+TGG + chr2_1:69504669-69504688 Msa0220970:intron 30.0%
ACAGCTACACATGAAAATTA+CGG - chr2_1:69504718-69504737 None:intergenic 30.0%
!! TTTGGTTTGACAGTAAAGTT+TGG - chr2_1:69504748-69504767 None:intergenic 30.0%
!! AAATAAGGGTGATGTTTTTG+TGG + chr2_1:69504782-69504801 Msa0220970:intron 30.0%
!! AATAAGGGTGATGTTTTTGT+GGG + chr2_1:69504783-69504802 Msa0220970:intron 30.0%
!! TAATTTTAAATGGTTTGCCC+GGG + chr2_1:69505065-69505084 Msa0220970:intron 30.0%
TCTGCTGTTGAAATAAGATA+TGG + chr2_1:69505334-69505353 Msa0220970:intron 30.0%
AAAATGTGTTTGTGCAGAAT+AGG + chr2_1:69505393-69505412 Msa0220970:intron 30.0%
! TGACATCAATTTTCACAAGA+TGG + chr2_1:69505519-69505538 Msa0220970:CDS 30.0%
GAGAGAGAGAGAAATGAAAA+TGG - chr2_1:69502917-69502936 None:intergenic 35.0%
! TCTGCAAACAAACTGGTTTA+TGG + chr2_1:69502937-69502956 Msa0220970:five_prime_UTR 35.0%
GTAGATGTGGTTGAAGTTAA+TGG - chr2_1:69502984-69503003 None:intergenic 35.0%
GAAGAAGAAGAAGAAAGAGA+AGG - chr2_1:69503058-69503077 None:intergenic 35.0%
! CTAGTGGATGATTATGATCA+TGG + chr2_1:69503527-69503546 Msa0220970:exon 35.0%
!!! GCTTGTATTTTAGGATTTGG+AGG + chr2_1:69503768-69503787 Msa0220970:intron 35.0%
!!! ATGCGTGTATCTTTTGAGTT+TGG + chr2_1:69503811-69503830 Msa0220970:intron 35.0%
! CTTTGTGGATTATGTATGCT+AGG + chr2_1:69504116-69504135 Msa0220970:intron 35.0%
TCCTTAGATATCCTTAAGGT+TGG + chr2_1:69504286-69504305 Msa0220970:intron 35.0%
CCTTAGATATCCTTAAGGTT+GGG + chr2_1:69504287-69504306 Msa0220970:intron 35.0%
TTTGTTTAGGGGATACAAGT+AGG + chr2_1:69504567-69504586 Msa0220970:intron 35.0%
! TAGCTTTTACCAAATCACAG+TGG - chr2_1:69504664-69504683 None:intergenic 35.0%
!! ATTTTGATCCGGTGTATGTT+TGG - chr2_1:69504766-69504785 None:intergenic 35.0%
ACATACACCGGATCAAAATA+AGG + chr2_1:69504767-69504786 Msa0220970:intron 35.0%
CATACACCGGATCAAAATAA+GGG + chr2_1:69504768-69504787 Msa0220970:intron 35.0%
!!! ACATCACCCTTATTTTGATC+CGG - chr2_1:69504777-69504796 None:intergenic 35.0%
!! ATAAGGGTGATGTTTTTGTG+GGG + chr2_1:69504784-69504803 Msa0220970:intron 35.0%
!!! TGTCTTTGTTGTTTTGTGCT+TGG + chr2_1:69504847-69504866 Msa0220970:intron 35.0%
TTCACGTATTTAAGCCACAA+TGG - chr2_1:69505236-69505255 None:intergenic 35.0%
GTTTATGAAATTGCCCATTG+TGG + chr2_1:69505264-69505283 Msa0220970:intron 35.0%
TCACGTATTTAAGCCACAAT+GGG - chr2_1:69505235-69505254 None:intergenic 35.0%
TTCACGTATTTAAGCCACAA+TGG - chr2_1:69505236-69505255 None:intergenic 35.0%
CAGATGTAACATAGCATGAT+AGG - chr2_1:69505318-69505337 None:intergenic 35.0%
AAATTGATGTCAGTTCCAGA+GGG - chr2_1:69505511-69505530 None:intergenic 35.0%
AAAATTGATGTCAGTTCCAG+AGG - chr2_1:69505512-69505531 None:intergenic 35.0%
GCAAGAAGAAGAAAGAAGGA+AGG - chr2_1:69502866-69502885 None:intergenic 40.0%
!! TGGGTTTGTTATCGTAGATG+TGG - chr2_1:69502997-69503016 None:intergenic 40.0%
!!! GTTTTGGTGGTGGTTGTTAT+GGG - chr2_1:69503016-69503035 None:intergenic 40.0%
!!! CTTTGAAGCGTTGTTGGTTT+TGG - chr2_1:69503032-69503051 None:intergenic 40.0%
! GTTGGATGTGAAATGGGTTT+GGG - chr2_1:69503163-69503182 None:intergenic 40.0%
AGGAGGATTAGATTGCTTTG+TGG + chr2_1:69504101-69504120 Msa0220970:intron 40.0%
CCCAACCTTAAGGATATCTA+AGG - chr2_1:69504290-69504309 None:intergenic 40.0%
! TGAGTGCATGTTTGGATTAG+CGG + chr2_1:69504612-69504631 Msa0220970:intron 40.0%
! TTTGGAGCTACCCAAAATCA+TGG + chr2_1:69504687-69504706 Msa0220970:intron 40.0%
CTGTCAAACCAAACATACAC+CGG + chr2_1:69504755-69504774 Msa0220970:intron 40.0%
TTGTGGGGTTCTTGATTAAG+AGG + chr2_1:69504799-69504818 Msa0220970:intron 40.0%
AAGAGGCAGAGTTACTAATG+TGG + chr2_1:69504816-69504835 Msa0220970:intron 40.0%
AGAGGCAGAGTTACTAATGT+GGG + chr2_1:69504817-69504836 Msa0220970:intron 40.0%
TTTGGATGAAGGGTGAAATG+AGG - chr2_1:69504949-69504968 None:intergenic 40.0%
CGGTTACAAATTGCGATGTT+AGG - chr2_1:69505595-69505614 None:intergenic 40.0%
CTCTCTCTCTGCAAACAAAC+TGG + chr2_1:69502930-69502949 Msa0220970:five_prime_UTR 45.0%
!!! GGTTTTGGTGGTGGTTGTTA+TGG - chr2_1:69503017-69503036 None:intergenic 45.0%
!!! TGAAGCGTTGTTGGTTTTGG+TGG - chr2_1:69503029-69503048 None:intergenic 45.0%
!! AGGGTTCTTTGAAGCGTTGT+TGG - chr2_1:69503038-69503057 None:intergenic 45.0%
GGAAACTGCAGAATACGAAG+CGG - chr2_1:69503095-69503114 None:intergenic 45.0%
GTTTCCGCACCAAACAAACA+TGG + chr2_1:69503109-69503128 Msa0220970:five_prime_UTR 45.0%
AGGATTCGGCCATGTTTGTT+TGG - chr2_1:69503121-69503140 None:intergenic 45.0%
GTCGGGTTAAAACGAGGATT+CGG - chr2_1:69503135-69503154 None:intergenic 45.0%
! TGTGAAATGGGTTTGGGTGT+GGG - chr2_1:69503157-69503176 None:intergenic 45.0%
! ATGTGAAATGGGTTTGGGTG+TGG - chr2_1:69503158-69503177 None:intergenic 45.0%
! GGTTGGATGTGAAATGGGTT+TGG - chr2_1:69503164-69503183 None:intergenic 45.0%
! TGTTGTTGTTGGATCGGGTT+GGG - chr2_1:69503211-69503230 None:intergenic 45.0%
AAACTGGAGGCTGTTGTTGT+TGG - chr2_1:69503222-69503241 None:intergenic 45.0%
!! AAAGAACCGATTCGACCCAA+CGG - chr2_1:69503295-69503314 None:intergenic 45.0%
! TCGAATCGGTTCTTTGTCGT+CGG + chr2_1:69503300-69503319 Msa0220970:five_prime_UTR 45.0%
!!! TTACCGAACCCGGTTTTGAA+CGG + chr2_1:69503328-69503347 Msa0220970:five_prime_UTR 45.0%
TCTTTCTCGCCGTTCAAAAC+CGG - chr2_1:69503340-69503359 None:intergenic 45.0%
CGAGAAAGAGAAGAGTTTGC+CGG + chr2_1:69503351-69503370 Msa0220970:five_prime_UTR 45.0%
AAGAGTTTGCCGGTGAAAAG+TGG + chr2_1:69503361-69503380 Msa0220970:five_prime_UTR 45.0%
AGAGGATGAGGAAGGTTTCT+GGG + chr2_1:69503473-69503492 Msa0220970:five_prime_UTR 45.0%
! GAAGGTTTCTGGGTCTGATA+CGG + chr2_1:69503483-69503502 Msa0220970:five_prime_UTR 45.0%
! AAGGTTTCTGGGTCTGATAC+GGG + chr2_1:69503484-69503503 Msa0220970:five_prime_UTR 45.0%
!! GAGTTGCAAGGGAGTATCTT+AGG + chr2_1:69504072-69504091 Msa0220970:intron 45.0%
!! CAAGGGAGTATCTTAGGACT+TGG + chr2_1:69504078-69504097 Msa0220970:intron 45.0%
AGTATCTTAGGACTTGGAGG+AGG + chr2_1:69504084-69504103 Msa0220970:intron 45.0%
! TACGGCGACACCATGATTTT+GGG - chr2_1:69504700-69504719 None:intergenic 45.0%
! TTACGGCGACACCATGATTT+TGG - chr2_1:69504701-69504720 None:intergenic 45.0%
CAACCAGATGCACAAAGCTT+TGG + chr2_1:69505210-69505229 Msa0220970:intron 45.0%
GTACATATCCTTTGTCCCTC+TGG + chr2_1:69505493-69505512 Msa0220970:CDS 45.0%
! GGTGTTGAGAATCTGTCTTC+TGG - chr2_1:69505574-69505593 None:intergenic 45.0%
!!! CTTTATTTAATAATTTTAAA+TGG + chr2_1:69505055-69505074 Msa0220970:intron 5.0%
!!! AGCGTTGTTGGTTTTGGTGG+TGG - chr2_1:69503026-69503045 None:intergenic 50.0%
AGAATACGAAGCGGAGGAGA+AGG - chr2_1:69503086-69503105 None:intergenic 50.0%
AACTGCAGAATACGAAGCGG+AGG - chr2_1:69503092-69503111 None:intergenic 50.0%
! TCGGCCATGTTTGTTTGGTG+CGG - chr2_1:69503116-69503135 None:intergenic 50.0%
! AAATGGGTTTGGGTGTGGGT+CGG - chr2_1:69503153-69503172 None:intergenic 50.0%
GGTGGGGTTGGATGTGAAAT+GGG - chr2_1:69503169-69503188 None:intergenic 50.0%
!! TGTTGGATCGGGTTGGGTTT+GGG - chr2_1:69503205-69503224 None:intergenic 50.0%
!! TTGTTGGATCGGGTTGGGTT+TGG - chr2_1:69503206-69503225 None:intergenic 50.0%
! CTGTTGTTGTTGGATCGGGT+TGG - chr2_1:69503212-69503231 None:intergenic 50.0%
! GAGGCTGTTGTTGTTGGATC+GGG - chr2_1:69503216-69503235 None:intergenic 50.0%
! GGAGGCTGTTGTTGTTGGAT+CGG - chr2_1:69503217-69503236 None:intergenic 50.0%
GGTCGATCTGAAGAAACTGG+AGG - chr2_1:69503235-69503254 None:intergenic 50.0%
CAGTTTCTTCAGATCGACCC+CGG + chr2_1:69503236-69503255 Msa0220970:five_prime_UTR 50.0%
AGCGAGATTGAATCTGGTCC+CGG - chr2_1:69503268-69503287 None:intergenic 50.0%
GGACGAAGCGAGATTGAATC+TGG - chr2_1:69503274-69503293 None:intergenic 50.0%
TTCAATCTCGCTTCGTCCGT+TGG + chr2_1:69503276-69503295 Msa0220970:five_prime_UTR 50.0%
TCAATCTCGCTTCGTCCGTT+GGG + chr2_1:69503277-69503296 Msa0220970:five_prime_UTR 50.0%
! CGAATCGGTTCTTTGTCGTC+GGG + chr2_1:69503301-69503320 Msa0220970:five_prime_UTR 50.0%
CTTTCTCGCCGTTCAAAACC+GGG - chr2_1:69503339-69503358 None:intergenic 50.0%
GAAAACTTGCGACCCAAGTC+TGG + chr2_1:69503442-69503461 Msa0220970:five_prime_UTR 50.0%
CTCTCATTCCCACCAGACTT+GGG - chr2_1:69503457-69503476 None:intergenic 50.0%
TGGGAATGAGAGGATGAGGA+AGG + chr2_1:69503465-69503484 Msa0220970:five_prime_UTR 50.0%
GAGAGGATGAGGAAGGTTTC+TGG + chr2_1:69503472-69503491 Msa0220970:five_prime_UTR 50.0%
! TCTGGGTCTGATACGGGTTT+TGG + chr2_1:69503490-69503509 Msa0220970:five_prime_UTR 50.0%
! CTGGGTCTGATACGGGTTTT+GGG + chr2_1:69503491-69503510 Msa0220970:five_prime_UTR 50.0%
GGGAATCGACCCAATTCTAG+TGG + chr2_1:69503511-69503530 Msa0220970:five_prime_UTR 50.0%
!! GGGAGTATCTTAGGACTTGG+AGG + chr2_1:69504081-69504100 Msa0220970:intron 50.0%
!! GCATGTTTGGATTAGCGGTG+AGG + chr2_1:69504617-69504636 Msa0220970:intron 50.0%
CTTGGTGAGCCACTGTGATT+TGG + chr2_1:69504652-69504671 Msa0220970:intron 50.0%
GACTCAAACCCGGAACTAGT+CGG + chr2_1:69505087-69505106 Msa0220970:intron 50.0%
! TACTCCATCCGACTAGTTCC+GGG - chr2_1:69505098-69505117 None:intergenic 50.0%
! CTACTCCATCCGACTAGTTC+CGG - chr2_1:69505099-69505118 None:intergenic 50.0%
! TGGCCAAAGCTTTGTGCATC+TGG - chr2_1:69505216-69505235 None:intergenic 50.0%
! GCACAAAGCTTTGGCCATTG+TGG + chr2_1:69505219-69505238 Msa0220970:intron 50.0%
TGTCAGTTCCAGAGGGACAA+AGG - chr2_1:69505504-69505523 None:intergenic 50.0%
GTGTGGGTCGGGTTAAAACG+AGG - chr2_1:69503141-69503160 None:intergenic 55.0%
! AATGGGTTTGGGTGTGGGTC+GGG - chr2_1:69503152-69503171 None:intergenic 55.0%
GGGTGGGGTTGGATGTGAAA+TGG - chr2_1:69503170-69503189 None:intergenic 55.0%
GGTTTGGGCTGTGATTGTGC+GGG - chr2_1:69503190-69503209 None:intergenic 55.0%
GGGTTTGGGCTGTGATTGTG+CGG - chr2_1:69503191-69503210 None:intergenic 55.0%
CGGGGTCGATCTGAAGAAAC+TGG - chr2_1:69503238-69503257 None:intergenic 55.0%
AATCTGGTCCCGGTTCAGAC+CGG - chr2_1:69503258-69503277 None:intergenic 55.0%
CTTCGTCCGTTGGGTCGAAT+CGG + chr2_1:69503286-69503305 Msa0220970:five_prime_UTR 55.0%
! GTCGGGTCTTTTACCGAACC+CGG + chr2_1:69503318-69503337 Msa0220970:five_prime_UTR 55.0%
TCGCCGTTCAAAACCGGGTT+CGG - chr2_1:69503334-69503353 None:intergenic 55.0%
ACAAACCCCGGACGAAGATG+CGG - chr2_1:69503400-69503419 None:intergenic 55.0%
TCCCTAAGACACTCCTCCCT+CGG - chr2_1:69503424-69503443 None:intergenic 55.0%
AACTTGCGACCCAAGTCTGG+TGG + chr2_1:69503445-69503464 Msa0220970:five_prime_UTR 55.0%
ACTTGCGACCCAAGTCTGGT+GGG + chr2_1:69503446-69503465 Msa0220970:five_prime_UTR 55.0%
CCTCTCATTCCCACCAGACT+TGG - chr2_1:69503458-69503477 None:intergenic 55.0%
CCAAGTCTGGTGGGAATGAG+AGG + chr2_1:69503455-69503474 Msa0220970:five_prime_UTR 55.0%
CTGGTGGGAATGAGAGGATG+AGG + chr2_1:69503461-69503480 Msa0220970:five_prime_UTR 55.0%
GTGAGGTTGCCATGCTAGCT+TGG + chr2_1:69504634-69504653 Msa0220970:intron 55.0%
AGTGGCTCACCAAGCTAGCA+TGG - chr2_1:69504646-69504665 None:intergenic 55.0%
TAGTTCCGGGTTTGAGTCCC+GGG - chr2_1:69505085-69505104 None:intergenic 55.0%
CTAGTTCCGGGTTTGAGTCC+CGG - chr2_1:69505086-69505105 None:intergenic 55.0%
CAAACCCGGAACTAGTCGGA+TGG + chr2_1:69505091-69505110 Msa0220970:intron 55.0%
TGTGATTGTGCGGGTGGGGT+TGG - chr2_1:69503181-69503200 None:intergenic 60.0%
TGGGCTGTGATTGTGCGGGT+GGG - chr2_1:69503186-69503205 None:intergenic 60.0%
TTGGGCTGTGATTGTGCGGG+TGG - chr2_1:69503187-69503206 None:intergenic 60.0%
AGATCGACCCCGGTCTGAAC+CGG + chr2_1:69503246-69503265 Msa0220970:five_prime_UTR 60.0%
ATCTGGTCCCGGTTCAGACC+GGG - chr2_1:69503257-69503276 None:intergenic 60.0%
!! TGCCGAGGGAGGAGTGTCTT+AGG + chr2_1:69503419-69503438 Msa0220970:five_prime_UTR 60.0%
!! GCCGAGGGAGGAGTGTCTTA+GGG + chr2_1:69503420-69503439 Msa0220970:five_prime_UTR 60.0%
GTTTGCCCGGGACTCAAACC+CGG + chr2_1:69505077-69505096 Msa0220970:intron 60.0%
GGGCTGTGATTGTGCGGGTG+GGG - chr2_1:69503185-69503204 None:intergenic 65.0%
GATCGACCCCGGTCTGAACC+GGG + chr2_1:69503247-69503266 Msa0220970:five_prime_UTR 65.0%
TCTGGTCCCGGTTCAGACCG+GGG - chr2_1:69503256-69503275 None:intergenic 65.0%
TGTGCCGCCGCATCTTCGTC+CGG + chr2_1:69503390-69503409 Msa0220970:five_prime_UTR 65.0%
AACCCCGGACGAAGATGCGG+CGG - chr2_1:69503397-69503416 None:intergenic 65.0%
TTCGTCCGGGGTTTGTGCCG+AGG + chr2_1:69503404-69503423 Msa0220970:five_prime_UTR 65.0%
TCGTCCGGGGTTTGTGCCGA+GGG + chr2_1:69503405-69503424 Msa0220970:five_prime_UTR 65.0%
TCCTCCCTCGGCACAAACCC+CGG - chr2_1:69503412-69503431 None:intergenic 65.0%
GTGCCGCCGCATCTTCGTCC+GGG + chr2_1:69503391-69503410 Msa0220970:five_prime_UTR 70.0%
TGCCGCCGCATCTTCGTCCG+GGG + chr2_1:69503392-69503411 Msa0220970:five_prime_UTR 70.0%
TCCGGGGTTTGTGCCGAGGG+AGG + chr2_1:69503408-69503427 Msa0220970:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69502851 69505661 69502851 ID=Msa0220970;Name=Msa0220970
chr2_1 mRNA 69502851 69505661 69502851 ID=Msa0220970-mRNA-1;Parent=Msa0220970;Name=Msa0220970-mRNA-1;_AED=0.11;_eAED=0.15;_QI=683|0|0.5|1|0|0|2|0|64
chr2_1 exon 69502851 69503548 69502851 ID=Msa0220970-mRNA-1:exon:19690;Parent=Msa0220970-mRNA-1
chr2_1 exon 69505482 69505661 69505482 ID=Msa0220970-mRNA-1:exon:19691;Parent=Msa0220970-mRNA-1
chr2_1 five_prime_UTR 69502851 69503533 69502851 ID=Msa0220970-mRNA-1:five_prime_utr;Parent=Msa0220970-mRNA-1
chr2_1 CDS 69503534 69503548 69503534 ID=Msa0220970-mRNA-1:cds;Parent=Msa0220970-mRNA-1
chr2_1 CDS 69505482 69505661 69505482 ID=Msa0220970-mRNA-1:cds;Parent=Msa0220970-mRNA-1
Gene Sequence

>Msa0220970

ATGATTATGATCATGAACAGTTCTCTGTACATATCCTTTGTCCCTCTGGAACTGACATCAATTTTCACAAGATGGCATCTGAAATCATTGAAGATGAAACATGACCAGAAGACAGATTCTCAACACCTAACATCGCAATTTGTAACCGAAATTTGTAATGATTCACAAGATGATTTGGTTCTTGTTTACCACTGA
Protein sequence

>Msa0220970

MIMIMNSSLYISFVPLELTSIFTRWHLKSLKMKHDQKTDSQHLTSQFVTEICNDSQDDLVLVYH*