AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1423660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423660 MtrunA17_Chr1g0209151 93.891 753 14 3 1 723 1 751 0.0 1396
Msa1423660 MtrunA17_Chr6g0483611 37.286 759 304 32 14 723 4 639 3.61e-98 316
Msa1423660 MtrunA17_Chr8g0392371 37.694 451 169 22 26 468 18 364 2.82e-54 196
Msa1423660 MtrunA17_Chr8g0392371 35.429 175 91 10 564 721 417 586 2.56e-11 67.0
Msa1423660 MtrunA17_Chr4g0048541 47.959 196 89 6 24 216 6 191 2.62e-46 170
Msa1423660 MtrunA17_Chr2g0279951 46.099 141 65 3 27 159 8 145 3.85e-32 130
Msa1423660 MtrunA17_Chr4g0049441 47.143 140 61 5 27 159 9 142 1.40e-29 122
Msa1423660 MtrunA17_Chr2g0305141 40.719 167 82 5 54 211 170 328 1.15e-26 117
Msa1423660 MtrunA17_Chr2g0277031 35.669 157 74 4 53 194 11 155 5.92e-25 106
Msa1423660 MtrunA17_Chr4g0065211 38.235 170 91 6 54 214 151 315 8.03e-25 111
Msa1423660 MtrunA17_Chr5g0428011 47.826 115 52 4 51 162 23 132 6.47e-24 104
Msa1423660 MtrunA17_Chr6g0461841 50.000 108 47 3 54 160 190 291 9.39e-23 104
Msa1423660 MtrunA17_Chr5g0428071 34.286 210 115 7 28 218 9 214 1.62e-21 97.8
Msa1423660 MtrunA17_Chr2g0310761 44.340 106 54 2 54 159 168 268 9.07e-21 98.2
Msa1423660 MtrunA17_Chr8g0357611 42.857 105 55 2 55 159 179 278 1.54e-20 97.4
Msa1423660 MtrunA17_Chr2g0310781 41.228 114 62 2 46 159 142 250 6.81e-20 95.1
Msa1423660 MtrunA17_Chr4g0058551 43.590 117 60 3 46 162 2 112 1.01e-19 87.4
Msa1423660 MtrunA17_Chr5g0440771 42.342 111 56 3 54 160 3 109 1.17e-19 87.8
Msa1423660 MtrunA17_Chr5g0412711 40.336 119 67 2 53 171 14 128 1.21e-19 88.6
Msa1423660 MtrunA17_Chr1g0196631 42.857 105 55 2 54 158 193 292 1.33e-19 94.4
Msa1423660 MtrunA17_Chr3g0117061 40.833 120 65 3 52 171 6 119 5.50e-18 83.2
Msa1423660 MtrunA17_Chr4g0072741 44.340 106 54 3 54 159 51 151 1.22e-15 81.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1423660 AT2G01190.1 46.806 767 256 28 27 723 36 720 8.94e-150 453
Msa1423660 AT3G18230.1 41.441 444 193 13 32 466 38 423 1.20e-67 235
Msa1423660 AT4G05150.1 37.847 288 124 11 22 280 36 297 3.31e-39 152
Msa1423660 AT5G09620.1 50.676 148 62 4 27 166 8 152 3.13e-35 141
Msa1423660 AT5G09620.2 50.676 148 62 4 27 166 8 152 4.77e-35 140
Msa1423660 AT5G64430.1 48.993 149 59 4 27 161 8 153 1.75e-32 132
Msa1423660 AT1G25300.1 53.913 115 46 1 46 160 2 109 4.57e-32 126
Msa1423660 AT5G16220.1 48.462 130 56 3 35 162 19 139 7.06e-26 112
Msa1423660 AT5G49920.1 38.356 146 71 4 53 194 8 138 4.32e-25 106
Msa1423660 AT1G79570.3 35.882 170 93 5 51 220 173 326 1.76e-23 107
Msa1423660 AT3G46920.1 38.732 142 83 3 54 194 8 146 3.63e-23 105
Msa1423660 AT3G46920.2 38.732 142 83 3 54 194 74 212 3.70e-23 106
Msa1423660 AT1G79570.4 35.882 170 93 5 51 220 173 326 4.24e-23 105
Msa1423660 AT1G79570.5 35.882 170 93 5 51 220 173 326 4.24e-23 105
Msa1423660 AT1G79570.2 35.882 170 93 5 51 220 173 326 4.75e-23 105
Msa1423660 AT1G79570.1 35.882 170 93 5 51 220 173 326 4.75e-23 105
Msa1423660 AT1G04700.3 43.448 145 70 6 31 174 100 233 2.56e-22 103
Msa1423660 AT3G26510.1 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT3G26510.4 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT3G26510.3 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT3G26510.2 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT3G26510.6 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT3G26510.5 40.769 130 68 3 50 174 5 130 2.62e-22 95.9
Msa1423660 AT1G04700.1 43.448 145 70 6 31 174 100 233 3.30e-22 102
Msa1423660 AT1G04700.2 43.448 145 70 6 31 174 100 233 3.30e-22 102
Msa1423660 AT1G16270.1 44.444 108 55 2 52 159 162 264 7.82e-22 101
Msa1423660 AT1G16270.2 44.444 108 55 2 52 159 162 264 7.82e-22 101
Msa1423660 AT1G16270.3 44.444 108 55 2 52 159 162 264 7.82e-22 101
Msa1423660 AT3G24715.1 45.000 120 58 4 54 170 175 289 2.96e-21 100
Msa1423660 AT3G24715.3 45.000 120 58 4 54 170 175 289 2.96e-21 100
Msa1423660 AT3G24715.2 45.000 120 58 4 54 170 175 289 2.96e-21 100
Msa1423660 AT2G35050.1 43.243 111 57 3 51 161 173 277 3.77e-21 99.8
Msa1423660 AT2G35050.2 43.243 111 57 3 51 161 173 277 3.77e-21 99.8
Msa1423660 AT5G57610.1 38.849 139 79 4 54 192 23 155 3.93e-21 99.4
Msa1423660 AT1G70640.1 42.982 114 57 4 50 162 3 109 1.23e-19 87.4
Msa1423660 AT3G48240.1 43.103 116 57 2 53 168 12 118 4.40e-19 85.9
Msa1423660 AT5G63130.1 40.909 110 56 2 53 162 13 113 5.06e-14 71.6
Msa1423660 AT5G63130.2 40.909 110 56 2 53 162 13 113 5.06e-14 71.6

Find 223 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGACGAAACGGCACCGTTTA+TGG 0.170692 tig0025571:-4562 None:intergenic
GTTTCTCAACCTGGATCTTT+TGG 0.172762 tig0025571:+4705 Msa1423660:CDS
TTCTGTGCAGCAGGCTAATA+TGG 0.185433 tig0025571:+6015 Msa1423660:CDS
CCTTGTTGGGGCTGTCTTGC+AGG 0.210879 tig0025571:-5983 None:intergenic
TGGCGAATTTGCCTCCTATT+AGG 0.214995 tig0025571:+4826 Msa1423660:CDS
ATGGTGGTTCTGGTGGTGTT+AGG 0.215415 tig0025571:+4862 Msa1423660:CDS
CTCTCCAAAACCTTGCTTAA+TGG 0.228074 tig0025571:+4297 Msa1423660:CDS
GACTTCTTCGTCTCCGTCTT+TGG 0.229377 tig0025571:+4806 Msa1423660:CDS
AGCAGCCGTCATTTCAGTTT+TGG 0.244960 tig0025571:-6145 None:intergenic
GCAGCAGGATCAGAAGGTTT+TGG 0.258724 tig0025571:+4920 Msa1423660:CDS
GGCGAATTTGCCTCCTATTA+GGG 0.266693 tig0025571:+4827 Msa1423660:CDS
CTGGCAGGGGCAGCAGAATT+AGG 0.281688 tig0025571:-6282 None:intergenic
AGAAATATTGTAACCTTGTT+GGG 0.287856 tig0025571:-5996 None:intergenic
GGAATTGACTGAGCAGATTC+AGG 0.292958 tig0025571:-4489 None:intergenic
TCTTCCATTAAGCAAGGTTT+TGG 0.293356 tig0025571:-4301 None:intergenic
CTTCTCCTGATTCAGTTTCA+AGG 0.299311 tig0025571:+5321 Msa1423660:CDS
CTTGTGACTGTGCCTGAGAT+TGG 0.302518 tig0025571:-5235 None:intergenic
GGTTGACGGCGTTGGAGGTT+GGG 0.306728 tig0025571:-4016 None:intergenic
CAAATTGTTGTTGCTGCTGC+TGG 0.308116 tig0025571:-5754 None:intergenic
TCTTCAACATGCACCCTAAT+AGG 0.318542 tig0025571:-4840 None:intergenic
GTTGTTGGTGTTGGTGGTAT+TGG 0.331151 tig0025571:+4969 Msa1423660:CDS
ATGGAGACTTCTTCGTCCTT+TGG 0.332324 tig0025571:+4780 Msa1423660:CDS
GTATCCACCCTTGTAGTTCC+AGG 0.341602 tig0025571:-5641 None:intergenic
AGCATATTCATAAGCATAAC+TGG 0.343168 tig0025571:-6301 None:intergenic
GTCTCCATCATAGGCGAATC+AGG 0.346187 tig0025571:-4765 None:intergenic
GGTAGGGGAGCACTTCTAAT+GGG 0.347012 tig0025571:-6123 None:intergenic
GGGGAGGAATCAACGGAATC+GGG 0.352597 tig0025571:-4087 None:intergenic
GTCCCATGAGTCGTTGTTGC+GGG 0.355446 tig0025571:-4115 None:intergenic
CATGTTGAAGATGGTGGTTC+TGG 0.360128 tig0025571:+4852 Msa1423660:CDS
GGGTAGGGGAGCACTTCTAA+TGG 0.363591 tig0025571:-6124 None:intergenic
TAGTTGAGGTGTGGTGGTGG+TGG 0.370870 tig0025571:-4063 None:intergenic
CGGTTGACGGCGTTGGAGGT+TGG 0.372039 tig0025571:-4017 None:intergenic
ACTGCACATCAGACGGAGTT+TGG 0.374152 tig0025571:-4160 None:intergenic
ATTGGTTCTGCTGTGGTTGT+TGG 0.379755 tig0025571:+5080 Msa1423660:CDS
TGATGAACAGTTTGCTCAAA+TGG 0.382086 tig0025571:+4947 Msa1423660:CDS
GGATAGTATGTAGGAATTGC+AGG 0.382162 tig0025571:-5914 None:intergenic
CAGAAATATTGTAACCTTGT+TGG 0.382832 tig0025571:-5997 None:intergenic
CAGCAGCAGCAGTTTATTCA+TGG 0.391314 tig0025571:+5869 Msa1423660:CDS
TCATTTCATTTCATCGATCA+TGG 0.392855 tig0025571:+3984 Msa1423660:exon
CATCAGAGAATACCCTATTC+TGG 0.394884 tig0025571:-5109 None:intergenic
CCTGTACATGTTGCTGCACC+TGG 0.396429 tig0025571:-5697 None:intergenic
GCGGCGGTTGGTGTTCCAAT+TGG 0.402499 tig0025571:+5062 Msa1423660:CDS
TGGTGGTTCTGGTGGTGTTA+GGG 0.407877 tig0025571:+4863 Msa1423660:CDS
GGATGCTGTGCAGGAACTTC+CGG 0.414421 tig0025571:-6411 None:intergenic
TGCTGTTGATGCTGACTTGT+AGG 0.418339 tig0025571:-6213 None:intergenic
TGTTGGTGATTACCAGAATA+GGG 0.419360 tig0025571:+5097 Msa1423660:CDS
CTCCGTCTGATGTGCAGTTA+CGG 0.420983 tig0025571:+4165 Msa1423660:CDS
GCTCAAATGGTTGTTGGTGT+TGG 0.422104 tig0025571:+4960 Msa1423660:CDS
TGTTCCTGATTCGCCTATGA+TGG 0.422549 tig0025571:+4761 Msa1423660:CDS
TTGTGACTGTGCCTGAGATT+GGG 0.424469 tig0025571:-5234 None:intergenic
AACGAATCTAGGTCTTCGTT+TGG 0.425475 tig0025571:-4348 None:intergenic
GGTCTTGATGATGATGTTGC+TGG 0.426742 tig0025571:+4636 Msa1423660:CDS
CCTGATGGTGTTTCTCAACC+TGG 0.431504 tig0025571:+4696 Msa1423660:CDS
GTTGACGGCGTTGGAGGTTG+GGG 0.431698 tig0025571:-4015 None:intergenic
CAACCTCCAACGCCGTCAAC+CGG 0.431862 tig0025571:+4018 Msa1423660:CDS
AGGGGAGGAATCAACGGAAT+CGG 0.433576 tig0025571:-4088 None:intergenic
GCAAACCTTGAAACTGAATC+AGG 0.434694 tig0025571:-5326 None:intergenic
TTGTTGGTGATTACCAGAAT+AGG 0.438247 tig0025571:+5096 Msa1423660:CDS
GAGGCTGTTGTTGCTGCTGA+TGG 0.438597 tig0025571:-5781 None:intergenic
CCAACCGCCGCCAGCGTAGT+TGG 0.439807 tig0025571:-5050 None:intergenic
ACCTCGATTAAGCGACGAAA+CGG 0.444945 tig0025571:-4574 None:intergenic
ATTTGCTCTTGATAAACAAC+AGG 0.445416 tig0025571:-5608 None:intergenic
GCTGCTTACAGAGCTGCAAC+TGG 0.449004 tig0025571:+6165 Msa1423660:CDS
AGCTGCTACTATGATGCAAC+CGG 0.450983 tig0025571:+6392 Msa1423660:CDS
CTCGATCCTACTGTTAGATC+CGG 0.457694 tig0025571:+4522 Msa1423660:CDS
TGATGGGGCTGTTGCTGTGA+TGG 0.459372 tig0025571:-5944 None:intergenic
TAGTAGCAGCTGCCATGGTC+TGG 0.462175 tig0025571:-6380 None:intergenic
CCGTCTGACGGATGCTGTGC+AGG 0.467762 tig0025571:-6420 None:intergenic
TGGTTCTTTGATACCATAAA+CGG 0.472006 tig0025571:+4549 Msa1423660:CDS
TTACAATATTTCTGTGCAGC+AGG 0.475574 tig0025571:+6006 Msa1423660:CDS
GTAGGAACTTGCACAAATTG+AGG 0.475620 tig0025571:-6195 None:intergenic
TGGACAGAGGCTACAGGATG+AGG 0.477050 tig0025571:+4989 Msa1423660:CDS
TCAAGCTCAGGCCCAATCTC+AGG 0.477257 tig0025571:+5223 Msa1423660:CDS
TAAATCTTCATCGGTTGAAA+CGG 0.478449 tig0025571:-4376 None:intergenic
CAAATGGTTGTTGGTGTTGG+TGG 0.479436 tig0025571:+4963 Msa1423660:CDS
TGCGGCTGATGTTGAGGTTG+TGG 0.481004 tig0025571:-5821 None:intergenic
GCCGCCAGCGTAGTTGGCAC+TGG 0.484966 tig0025571:-5044 None:intergenic
CAGTTTGCTCAAATGGTTGT+TGG 0.485362 tig0025571:+4954 Msa1423660:CDS
GACGTAACAGAGAGCTTTGT+CGG 0.485469 tig0025571:-4211 None:intergenic
ACAACCACAGCAGAACCAAT+TGG 0.486447 tig0025571:-5077 None:intergenic
GGTCCGGTTGACGGCGTTGG+AGG 0.489230 tig0025571:-4021 None:intergenic
TACATAGTAAACCTGGTGAT+GGG 0.489469 tig0025571:-5960 None:intergenic
TTGGATGTGGCTGGAGGGAA+AGG 0.491131 tig0025571:-4141 None:intergenic
TTACAATTCCCAAAAGATCC+AGG 0.491686 tig0025571:-4714 None:intergenic
GCAGCAGCAGCAGGATCAGA+AGG 0.491807 tig0025571:+4914 Msa1423660:CDS
GGTGGTCCGGTTGACGGCGT+TGG 0.493691 tig0025571:-4024 None:intergenic
GTGTGGTGGTGGTGGAGCCA+CGG 0.495167 tig0025571:-4055 None:intergenic
GATAACGGTGGTCCGGTTGA+CGG 0.499187 tig0025571:-4030 None:intergenic
GGGTAGTTGAGGTGTGGTGG+TGG 0.500900 tig0025571:-4066 None:intergenic
TGAGAGTAGTATACTTGAGC+AGG 0.501127 tig0025571:-6327 None:intergenic
CGATCCACATGGTAGGATCC+AGG 0.502009 tig0025571:+5679 Msa1423660:CDS
GGTGGAGCCACGGAGGATAA+CGG 0.503298 tig0025571:-4045 None:intergenic
TGCTGCTTCATGCCGTCTGA+CGG 0.504939 tig0025571:-6432 None:intergenic
TGTTGCTGTGATGGATATAC+AGG 0.506750 tig0025571:-5935 None:intergenic
CACGGAGGATAACGGTGGTC+CGG 0.508705 tig0025571:-4037 None:intergenic
GACGAAGAAGTCTCCATCAT+AGG 0.509913 tig0025571:-4774 None:intergenic
GAATTGTAAGAATTTGAAGC+AGG 0.512443 tig0025571:+4728 Msa1423660:CDS
AAAGTCAAGTGGCGGTGGCT+CGG 0.513401 tig0025571:+5292 Msa1423660:CDS
CATATTGAACAAGGTTCCAC+TGG 0.513758 tig0025571:+4666 Msa1423660:CDS
TGGTACTGTGAAGGCATTGT+GGG 0.515032 tig0025571:-6360 None:intergenic
CAGCAACATGTACAGGATCC+TGG 0.517129 tig0025571:+5704 Msa1423660:CDS
ACCTGTGGCTGAGGAGTTGG+TGG 0.517587 tig0025571:-5173 None:intergenic
TCAAGCTCAAAATCAAGCTC+AGG 0.519294 tig0025571:+5211 Msa1423660:CDS
TCGGGGTAGTTGAGGTGTGG+TGG 0.520239 tig0025571:-4069 None:intergenic
GTAGTTGGCACTGGAGGATG+AGG 0.520654 tig0025571:-5035 None:intergenic
AGCAGCAGCAGTTTATTCAT+GGG 0.521183 tig0025571:+5870 Msa1423660:CDS
CCAACTACGCTGGCGGCGGT+TGG 0.521638 tig0025571:+5050 Msa1423660:CDS
TATTCATAAGCATAACTGGC+AGG 0.521937 tig0025571:-6297 None:intergenic
TGCTGCTGCGGCTGATGTTG+AGG 0.522699 tig0025571:-5827 None:intergenic
CCAGGTGCAGCAACATGTAC+AGG 0.525477 tig0025571:+5697 Msa1423660:CDS
GGCAGCAGAATTAGGAGCCA+TGG 0.527937 tig0025571:-6274 None:intergenic
AGACGGAGTTTGGATGTGGC+TGG 0.531377 tig0025571:-4150 None:intergenic
GAAGGCATTGTGGGCGCCAT+AGG 0.531634 tig0025571:-6351 None:intergenic
CAACAAAAGTCAAGTGGCGG+TGG 0.531834 tig0025571:+5287 Msa1423660:CDS
GTTCCCGCAACAACGACTCA+TGG 0.532318 tig0025571:+4112 Msa1423660:CDS
AGCTGATACTTGAAAGTGAA+AGG 0.533088 tig0025571:-4324 None:intergenic
TAACAGAGAGCTTTGTCGGT+TGG 0.534354 tig0025571:-4207 None:intergenic
CCACCATCCGTCTCAGTCCA+TGG 0.536469 tig0025571:+6257 Msa1423660:CDS
AGAGCTTTGTCGGTTGGACG+AGG 0.539750 tig0025571:-4201 None:intergenic
AGTATACTACTCTCAGCCTA+TGG 0.542313 tig0025571:+6335 Msa1423660:CDS
CGTCCCATGAGTCGTTGTTG+CGG 0.542603 tig0025571:-4116 None:intergenic
AGTGCCAACTACGCTGGCGG+CGG 0.544621 tig0025571:+5046 Msa1423660:CDS
AAATTCAACCTGGAACTACA+AGG 0.546548 tig0025571:+5633 Msa1423660:CDS
TGGTATTGGACAGAGGCTAC+AGG 0.548332 tig0025571:+4983 Msa1423660:CDS
CGCCGTAACTGCACATCAGA+CGG 0.548433 tig0025571:-4167 None:intergenic
GAAAGGTCTTCCATTAAGCA+AGG 0.551628 tig0025571:-4307 None:intergenic
CATCATAGTAGCAGCTGCCA+TGG 0.552855 tig0025571:-6385 None:intergenic
TGAGCAGATTCAGGTTTCGA+TGG 0.553906 tig0025571:-4480 None:intergenic
CCAGGTTGAGAAACACCATC+AGG 0.555428 tig0025571:-4696 None:intergenic
GTAGGGGAGCACTTCTAATG+GGG 0.555535 tig0025571:-6122 None:intergenic
ACCACCAACTCCTCAGCCAC+AGG 0.556074 tig0025571:+5172 Msa1423660:CDS
TCTGTGCAGCAGGCTAATAT+GGG 0.556647 tig0025571:+6016 Msa1423660:CDS
GGAACGAGGGGAGGAATCAA+CGG 0.556888 tig0025571:-4094 None:intergenic
CAACGGAATCGGGGTAGTTG+AGG 0.557276 tig0025571:-4077 None:intergenic
ATTCTCTGATGATGAGAGAT+CGG 0.557288 tig0025571:+5121 Msa1423660:CDS
GGATATACAGGATAGTATGT+AGG 0.557743 tig0025571:-5923 None:intergenic
TCCTCCAGTGCCAACTACGC+TGG 0.559724 tig0025571:+5040 Msa1423660:CDS
TGCTGTTGCAGCAAATAACC+AGG 0.561601 tig0025571:-5722 None:intergenic
CTACTGTTAGATCCGGTGAA+TGG 0.563307 tig0025571:+4529 Msa1423660:CDS
GGACAGAGGCTACAGGATGA+GGG 0.567639 tig0025571:+4990 Msa1423660:CDS
GGTTGCTGCGGCTGATGTTG+AGG 0.576485 tig0025571:-5800 None:intergenic
GTCTTGATGATGATGTTGCT+GGG 0.576576 tig0025571:+4637 Msa1423660:CDS
GTTGAAGATGGTGGTTCTGG+TGG 0.576663 tig0025571:+4855 Msa1423660:CDS
AAACGGAGATTAACGAATCT+AGG 0.578203 tig0025571:-4359 None:intergenic
ACAACCTGTGGCTGAGGAGT+TGG 0.580416 tig0025571:-5176 None:intergenic
GGATAAATCCGCGAGTGAGG+TGG 0.580510 tig0025571:-4268 None:intergenic
ACTTGAACAACCTGTGGCTG+AGG 0.580822 tig0025571:-5182 None:intergenic
GCCGTTTCGTCGCTTAATCG+AGG 0.581594 tig0025571:+4573 Msa1423660:CDS
GTACATAGTAAACCTGGTGA+TGG 0.582079 tig0025571:-5961 None:intergenic
CCATGGACTGAGACGGATGG+TGG 0.582286 tig0025571:-6257 None:intergenic
ATTAGGGTGCATGTTGAAGA+TGG 0.582359 tig0025571:+4843 Msa1423660:CDS
GAAATATTGTAACCTTGTTG+GGG 0.583775 tig0025571:-5995 None:intergenic
GAGTCGTTGTTGCGGGAACG+AGG 0.584939 tig0025571:-4108 None:intergenic
TTCCCGCAACAACGACTCAT+GGG 0.585174 tig0025571:+4113 Msa1423660:CDS
GTCGTTGTTGCGGGAACGAG+GGG 0.585961 tig0025571:-4106 None:intergenic
ATCGGATCATGGTGTGCCTG+TGG 0.586106 tig0025571:+5139 Msa1423660:CDS
ATTCATAAGCATAACTGGCA+GGG 0.588015 tig0025571:-6296 None:intergenic
GGAGGCAAATTCGCCAAAGA+CGG 0.588179 tig0025571:-4819 None:intergenic
CCTACCCAAAACTGAAATGA+CGG 0.588235 tig0025571:+6140 Msa1423660:CDS
TTCATAAGCATAACTGGCAG+GGG 0.590768 tig0025571:-6295 None:intergenic
TCCACTGGAGAAGCTCCTGA+TGG 0.593224 tig0025571:+4681 Msa1423660:CDS
TGAAGCTGTTGTTGCTGCTG+CGG 0.593547 tig0025571:-5839 None:intergenic
TGTTCCAATTGGTTCTGCTG+TGG 0.593696 tig0025571:+5073 Msa1423660:CDS
AGACGAAGAAGTCGAACCAA+AGG 0.594657 tig0025571:-4796 None:intergenic
GACAAAGCTCTCTGTTACGT+CGG 0.596356 tig0025571:+4213 Msa1423660:CDS
ACAGCAACAGCCCCATCACC+AGG 0.596464 tig0025571:+5949 Msa1423660:CDS
CGGAGTTTGGATGTGGCTGG+AGG 0.598235 tig0025571:-4147 None:intergenic
TGAACGCGCCACCTCACTCG+CGG 0.598732 tig0025571:+4260 Msa1423660:CDS
AGTCGTTGTTGCGGGAACGA+GGG 0.600229 tig0025571:-4107 None:intergenic
GCCAGCGTAGTTGGCACTGG+AGG 0.600507 tig0025571:-5041 None:intergenic
ACATAGTAAACCTGGTGATG+GGG 0.601720 tig0025571:-5959 None:intergenic
CATCAGACGGAGTTTGGATG+TGG 0.601884 tig0025571:-4154 None:intergenic
GCAGCAGCAGTTTATTCATG+GGG 0.604368 tig0025571:+5871 Msa1423660:CDS
GTTGTTGCGGGAACGAGGGG+AGG 0.604508 tig0025571:-4103 None:intergenic
CTTGAAACTGAATCAGGAGA+AGG 0.605496 tig0025571:-5320 None:intergenic
CTGGTACTGTGAAGGCATTG+TGG 0.606843 tig0025571:-6361 None:intergenic
GATGATGAGAGATCGGATCA+TGG 0.609090 tig0025571:+5128 Msa1423660:CDS
CTGGATCCTACCATGTGGAT+CGG 0.610151 tig0025571:-5678 None:intergenic
GGAGTTTGGATGTGGCTGGA+GGG 0.611331 tig0025571:-4146 None:intergenic
TTGCAGGTACATAGTAAACC+TGG 0.612429 tig0025571:-5967 None:intergenic
CCTGCACAGCATCCGTCAGA+CGG 0.613220 tig0025571:+6420 Msa1423660:CDS
CGTGGATAAATCCGCGAGTG+AGG 0.614369 tig0025571:-4271 None:intergenic
GATAGTATGTAGGAATTGCA+GGG 0.616127 tig0025571:-5913 None:intergenic
GGGAGGAATCAACGGAATCG+GGG 0.618967 tig0025571:-4086 None:intergenic
GGTATCAAAGAACCATTCAC+CGG 0.620315 tig0025571:-4541 None:intergenic
TCGGATCATGGTGTGCCTGT+GGG 0.622213 tig0025571:+5140 Msa1423660:CDS
GGGAATAATCATATTGAACA+AGG 0.623357 tig0025571:+4657 Msa1423660:CDS
TCAACATGCACCCTAATAGG+AGG 0.625269 tig0025571:-4837 None:intergenic
ATCAATTTGTCCGATCCACA+TGG 0.627947 tig0025571:+5668 Msa1423660:CDS
CATTCACCGGATCTAACAGT+AGG 0.628644 tig0025571:-4528 None:intergenic
GAGCAAATTCAAATTCAACC+TGG 0.628952 tig0025571:+5623 Msa1423660:CDS
ACAGTAGGATCGAGAATTTG+CGG 0.630234 tig0025571:-4513 None:intergenic
TCCAGTGCCAACTACGCTGG+CGG 0.630709 tig0025571:+5043 Msa1423660:CDS
GCCATGGTCTGGTACTGTGA+AGG 0.631831 tig0025571:-6369 None:intergenic
TTAGGAGCCATGGACTGAGA+CGG 0.636364 tig0025571:-6264 None:intergenic
CCTGCAAGACAGCCCCAACA+AGG 0.639304 tig0025571:+5983 Msa1423660:CDS
ACCATCAGGAGCTTCTCCAG+TGG 0.641015 tig0025571:-4682 None:intergenic
ATTTGTCCGATCCACATGGT+AGG 0.641689 tig0025571:+5672 Msa1423660:CDS
GCTTACAGAGCTGCAACTGG+AGG 0.643603 tig0025571:+6168 Msa1423660:CDS
TGGTGGTGGTGGAGCCACGG+AGG 0.644740 tig0025571:-4052 None:intergenic
CAATTTCAACAAAAGTCAAG+TGG 0.647060 tig0025571:+5281 Msa1423660:CDS
GAGCTTTGTCGGTTGGACGA+GGG 0.650315 tig0025571:-4200 None:intergenic
TCAACCTGGAACTACAAGGG+TGG 0.652554 tig0025571:+5637 Msa1423660:CDS
GTGTTGGTGGTATTGGACAG+AGG 0.654498 tig0025571:+4976 Msa1423660:CDS
CGGTTGGACGAGGGACGATG+TGG 0.660936 tig0025571:-4191 None:intergenic
GTTGGCACTGGAGGATGAGG+CGG 0.662967 tig0025571:-5032 None:intergenic
AAAGCTCTCTGTTACGTCGG+CGG 0.665892 tig0025571:+4216 Msa1423660:CDS
GGTTGAACTTGAACAACCTG+TGG 0.669833 tig0025571:-5188 None:intergenic
GAATCGGGGTAGTTGAGGTG+TGG 0.676463 tig0025571:-4072 None:intergenic
GAGCCATGGACTGAGACGGA+TGG 0.677383 tig0025571:-6260 None:intergenic
TTTCAACAAAAGTCAAGTGG+CGG 0.678298 tig0025571:+5284 Msa1423660:CDS
AATTCAACCTGGAACTACAA+GGG 0.679111 tig0025571:+5634 Msa1423660:CDS
AGGGTGCATGTTGAAGATGG+TGG 0.679137 tig0025571:+4846 Msa1423660:CDS
TTTCATTTCATCGATCATGG+AGG 0.692878 tig0025571:+3987 Msa1423660:exon
TGTTGAGGTTGTGGTTGCTG+CGG 0.693955 tig0025571:-5812 None:intergenic
CGGACCACCGTTATCCTCCG+TGG 0.696266 tig0025571:+4038 Msa1423660:CDS
CAGCAGCAGTTTATTCATGG+GGG 0.698822 tig0025571:+5872 Msa1423660:CDS
GCCTTCACAGTACCAGACCA+TGG 0.702903 tig0025571:+6368 Msa1423660:CDS
GTAGGATCGAGAATTTGCGG+TGG 0.710077 tig0025571:-4510 None:intergenic
CGTCTGATGTGCAGTTACGG+CGG 0.713858 tig0025571:+4168 Msa1423660:CDS
ACTTGCTTATAGTGGCTGTG+AGG 0.735355 tig0025571:-6463 None:intergenic
GGAGCCACGGAGGATAACGG+TGG 0.735804 tig0025571:-4042 None:intergenic
TGCACCTGGATCCTACCATG+TGG 0.740835 tig0025571:-5683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAATGTTTGTTTACAT+AGG + tig0025571:5411-5430 Msa1423660:intron 15.0%
!!! CATATTTTCTAAATCTTCAT+CGG - tig0025571:4388-4407 None:intergenic 20.0%
!!! TCTGTTTTATATTTGTGATA+TGG + tig0025571:5384-5403 Msa1423660:intron 20.0%
! AAATAAAAGGACGAAAAAGA+GGG - tig0025571:3892-3911 None:intergenic 25.0%
! AAAATAAAAGGACGAAAAAG+AGG - tig0025571:3893-3912 None:intergenic 25.0%
! GAATTACAAAAACAGGTAAA+TGG - tig0025571:5364-5383 None:intergenic 25.0%
! AAACAGAGAATTACAAAAAC+AGG - tig0025571:5371-5390 None:intergenic 25.0%
! TGATTATTATAAACCTAGAG+TGG + tig0025571:5496-5515 Msa1423660:intron 25.0%
! AGAAATATTGTAACCTTGTT+GGG - tig0025571:5999-6018 None:intergenic 25.0%
! TTTTGCAGCGCAAAAATAAA+AGG - tig0025571:3905-3924 None:intergenic 30.0%
!!! TTTATTTTTGCGCTGCAAAA+AGG + tig0025571:3905-3924 Msa1423660:five_prime_UTR 30.0%
!!! TTATTTTTGCGCTGCAAAAA+GGG + tig0025571:3906-3925 Msa1423660:five_prime_UTR 30.0%
TCATTTCATTTCATCGATCA+TGG + tig0025571:3984-4003 Msa1423660:exon 30.0%
! TAAATCTTCATCGGTTGAAA+CGG - tig0025571:4379-4398 None:intergenic 30.0%
TGGTTCTTTGATACCATAAA+CGG + tig0025571:4549-4568 Msa1423660:CDS 30.0%
! TCTTCTGTGAATCATCTTTT+AGG + tig0025571:4615-4634 Msa1423660:CDS 30.0%
GGGAATAATCATATTGAACA+AGG + tig0025571:4657-4676 Msa1423660:CDS 30.0%
! GAATTGTAAGAATTTGAAGC+AGG + tig0025571:4728-4747 Msa1423660:CDS 30.0%
! CTTGACTTTTGTTGAAATTG+AGG - tig0025571:5281-5300 None:intergenic 30.0%
CAATTTCAACAAAAGTCAAG+TGG + tig0025571:5281-5300 Msa1423660:CDS 30.0%
TTATTATAAACCTAGAGTGG+TGG + tig0025571:5499-5518 Msa1423660:intron 30.0%
! GGTTTAGCAATATCTGAAAT+AGG + tig0025571:5520-5539 Msa1423660:intron 30.0%
ATTTGCTCTTGATAAACAAC+AGG - tig0025571:5611-5630 None:intergenic 30.0%
GAAATATTGTAACCTTGTTG+GGG - tig0025571:5998-6017 None:intergenic 30.0%
CAGAAATATTGTAACCTTGT+TGG - tig0025571:6000-6019 None:intergenic 30.0%
AGCATATTCATAAGCATAAC+TGG - tig0025571:6304-6323 None:intergenic 30.0%
!! TATTTTTGCGCTGCAAAAAG+GGG + tig0025571:3907-3926 Msa1423660:five_prime_UTR 35.0%
TTTCATTTCATCGATCATGG+AGG + tig0025571:3987-4006 Msa1423660:exon 35.0%
! TCTTCCATTAAGCAAGGTTT+TGG - tig0025571:4304-4323 None:intergenic 35.0%
AGCTGATACTTGAAAGTGAA+AGG - tig0025571:4327-4346 None:intergenic 35.0%
AAACGGAGATTAACGAATCT+AGG - tig0025571:4362-4381 None:intergenic 35.0%
! TTTCTCAACCTGGATCTTTT+GGG + tig0025571:4706-4725 Msa1423660:CDS 35.0%
TTACAATTCCCAAAAGATCC+AGG - tig0025571:4717-4736 None:intergenic 35.0%
! TGATGAACAGTTTGCTCAAA+TGG + tig0025571:4947-4966 Msa1423660:CDS 35.0%
!! TTGTTGGTGATTACCAGAAT+AGG + tig0025571:5096-5115 Msa1423660:CDS 35.0%
!! TGTTGGTGATTACCAGAATA+GGG + tig0025571:5097-5116 Msa1423660:CDS 35.0%
! ATTCTCTGATGATGAGAGAT+CGG + tig0025571:5121-5140 Msa1423660:CDS 35.0%
TTTCAACAAAAGTCAAGTGG+CGG + tig0025571:5284-5303 Msa1423660:CDS 35.0%
! CAATATCTGAAATAGGCTGT+TGG + tig0025571:5527-5546 Msa1423660:intron 35.0%
GAGCAAATTCAAATTCAACC+TGG + tig0025571:5623-5642 Msa1423660:CDS 35.0%
AAATTCAACCTGGAACTACA+AGG + tig0025571:5633-5652 Msa1423660:CDS 35.0%
AATTCAACCTGGAACTACAA+GGG + tig0025571:5634-5653 Msa1423660:CDS 35.0%
GATAGTATGTAGGAATTGCA+GGG - tig0025571:5916-5935 None:intergenic 35.0%
GGATATACAGGATAGTATGT+AGG - tig0025571:5926-5945 None:intergenic 35.0%
TACATAGTAAACCTGGTGAT+GGG - tig0025571:5963-5982 None:intergenic 35.0%
! TTACAATATTTCTGTGCAGC+AGG + tig0025571:6006-6025 Msa1423660:CDS 35.0%
! TTTTGTTGAACCAATGTGGT+GGG - tig0025571:6090-6109 None:intergenic 35.0%
! ATTTTGTTGAACCAATGTGG+TGG - tig0025571:6091-6110 None:intergenic 35.0%
! TGCATTTTGTTGAACCAATG+TGG - tig0025571:6094-6113 None:intergenic 35.0%
ATTCATAAGCATAACTGGCA+GGG - tig0025571:6299-6318 None:intergenic 35.0%
TATTCATAAGCATAACTGGC+AGG - tig0025571:6300-6319 None:intergenic 35.0%
!!! CATTAAGCAAGGTTTTGGAG+AGG - tig0025571:4299-4318 None:intergenic 40.0%
CTCTCCAAAACCTTGCTTAA+TGG + tig0025571:4297-4316 Msa1423660:CDS 40.0%
GAAAGGTCTTCCATTAAGCA+AGG - tig0025571:4310-4329 None:intergenic 40.0%
AACGAATCTAGGTCTTCGTT+TGG - tig0025571:4351-4370 None:intergenic 40.0%
AGAAAAAGACGAATTCGCGA+GGG - tig0025571:4456-4475 None:intergenic 40.0%
ACAGTAGGATCGAGAATTTG+CGG - tig0025571:4516-4535 None:intergenic 40.0%
GGTATCAAAGAACCATTCAC+CGG - tig0025571:4544-4563 None:intergenic 40.0%
!! GTCTTGATGATGATGTTGCT+GGG + tig0025571:4637-4656 Msa1423660:CDS 40.0%
CATATTGAACAAGGTTCCAC+TGG + tig0025571:4666-4685 Msa1423660:CDS 40.0%
! GTTTCTCAACCTGGATCTTT+TGG + tig0025571:4705-4724 Msa1423660:CDS 40.0%
TCTTCAACATGCACCCTAAT+AGG - tig0025571:4843-4862 None:intergenic 40.0%
ATTAGGGTGCATGTTGAAGA+TGG + tig0025571:4843-4862 Msa1423660:CDS 40.0%
! CAGTTTGCTCAAATGGTTGT+TGG + tig0025571:4954-4973 Msa1423660:CDS 40.0%
CATCAGAGAATACCCTATTC+TGG - tig0025571:5112-5131 None:intergenic 40.0%
! TGAGCTTGATTTTGAGCTTG+AGG - tig0025571:5212-5231 None:intergenic 40.0%
TCAAGCTCAAAATCAAGCTC+AGG + tig0025571:5211-5230 Msa1423660:CDS 40.0%
CTTGAAACTGAATCAGGAGA+AGG - tig0025571:5323-5342 None:intergenic 40.0%
CTTCTCCTGATTCAGTTTCA+AGG + tig0025571:5321-5340 Msa1423660:CDS 40.0%
GCAAACCTTGAAACTGAATC+AGG - tig0025571:5329-5348 None:intergenic 40.0%
TATTGCTAAACCACCACTCT+AGG - tig0025571:5512-5531 None:intergenic 40.0%
! TATCTGAAATAGGCTGTTGG+TGG + tig0025571:5530-5549 Msa1423660:intron 40.0%
ATCAATTTGTCCGATCCACA+TGG + tig0025571:5668-5687 Msa1423660:CDS 40.0%
AGCAGCAGCAGTTTATTCAT+GGG + tig0025571:5870-5889 Msa1423660:CDS 40.0%
GGATAGTATGTAGGAATTGC+AGG - tig0025571:5917-5936 None:intergenic 40.0%
!! TGTTGCTGTGATGGATATAC+AGG - tig0025571:5938-5957 None:intergenic 40.0%
! ACATAGTAAACCTGGTGATG+GGG - tig0025571:5962-5981 None:intergenic 40.0%
GTACATAGTAAACCTGGTGA+TGG - tig0025571:5964-5983 None:intergenic 40.0%
TTGCAGGTACATAGTAAACC+TGG - tig0025571:5970-5989 None:intergenic 40.0%
TTTGTTGAACCAATGTGGTG+GGG - tig0025571:6089-6108 None:intergenic 40.0%
!!! GTCATTTCAGTTTTGGGTAG+GGG - tig0025571:6141-6160 None:intergenic 40.0%
!!! CGTCATTTCAGTTTTGGGTA+GGG - tig0025571:6142-6161 None:intergenic 40.0%
CCTACCCAAAACTGAAATGA+CGG + tig0025571:6140-6159 Msa1423660:CDS 40.0%
GTAGGAACTTGCACAAATTG+AGG - tig0025571:6198-6217 None:intergenic 40.0%
TTCATAAGCATAACTGGCAG+GGG - tig0025571:6298-6317 None:intergenic 40.0%
TGAGAGTAGTATACTTGAGC+AGG - tig0025571:6330-6349 None:intergenic 40.0%
AGTATACTACTCTCAGCCTA+TGG + tig0025571:6335-6354 Msa1423660:CDS 40.0%
!!! TTTTGTGCTGCTTGTTGCTG+CGG - tig0025571:3938-3957 None:intergenic 45.0%
! TAACAGAGAGCTTTGTCGGT+TGG - tig0025571:4210-4229 None:intergenic 45.0%
! GACGTAACAGAGAGCTTTGT+CGG - tig0025571:4214-4233 None:intergenic 45.0%
GACAAAGCTCTCTGTTACGT+CGG + tig0025571:4213-4232 Msa1423660:CDS 45.0%
CAGAAAAAGACGAATTCGCG+AGG - tig0025571:4457-4476 None:intergenic 45.0%
! TGAGCAGATTCAGGTTTCGA+TGG - tig0025571:4483-4502 None:intergenic 45.0%
GGAATTGACTGAGCAGATTC+AGG - tig0025571:4492-4511 None:intergenic 45.0%
CTCGATCCTACTGTTAGATC+CGG + tig0025571:4522-4541 Msa1423660:CDS 45.0%
CATTCACCGGATCTAACAGT+AGG - tig0025571:4531-4550 None:intergenic 45.0%
CTACTGTTAGATCCGGTGAA+TGG + tig0025571:4529-4548 Msa1423660:CDS 45.0%
ACCTCGATTAAGCGACGAAA+CGG - tig0025571:4577-4596 None:intergenic 45.0%
!! GGTCTTGATGATGATGTTGC+TGG + tig0025571:4636-4655 Msa1423660:CDS 45.0%
! TGTTCCTGATTCGCCTATGA+TGG + tig0025571:4761-4780 Msa1423660:CDS 45.0%
GACGAAGAAGTCTCCATCAT+AGG - tig0025571:4777-4796 None:intergenic 45.0%
ATGGAGACTTCTTCGTCCTT+TGG + tig0025571:4780-4799 Msa1423660:CDS 45.0%
AGACGAAGAAGTCGAACCAA+AGG - tig0025571:4799-4818 None:intergenic 45.0%
TGGCGAATTTGCCTCCTATT+AGG + tig0025571:4826-4845 Msa1423660:CDS 45.0%
GGCGAATTTGCCTCCTATTA+GGG + tig0025571:4827-4846 Msa1423660:CDS 45.0%
TCAACATGCACCCTAATAGG+AGG - tig0025571:4840-4859 None:intergenic 45.0%
! CATGTTGAAGATGGTGGTTC+TGG + tig0025571:4852-4871 Msa1423660:CDS 45.0%
! CAGCAGGATCAGAAGGTTTT+GGG + tig0025571:4921-4940 Msa1423660:CDS 45.0%
! AGCAGGATCAGAAGGTTTTG+GGG + tig0025571:4922-4941 Msa1423660:CDS 45.0%
GCTCAAATGGTTGTTGGTGT+TGG + tig0025571:4960-4979 Msa1423660:CDS 45.0%
!! CAAATGGTTGTTGGTGTTGG+TGG + tig0025571:4963-4982 Msa1423660:CDS 45.0%
!! GTTGTTGGTGTTGGTGGTAT+TGG + tig0025571:4969-4988 Msa1423660:CDS 45.0%
!! TGTTCCAATTGGTTCTGCTG+TGG + tig0025571:5073-5092 Msa1423660:CDS 45.0%
ACAACCACAGCAGAACCAAT+TGG - tig0025571:5080-5099 None:intergenic 45.0%
!! ATTGGTTCTGCTGTGGTTGT+TGG + tig0025571:5080-5099 Msa1423660:CDS 45.0%
GATGATGAGAGATCGGATCA+TGG + tig0025571:5128-5147 Msa1423660:CDS 45.0%
! GGTGGTTTTCTATATCCCAC+AGG - tig0025571:5158-5177 None:intergenic 45.0%
GGTTGAACTTGAACAACCTG+TGG - tig0025571:5191-5210 None:intergenic 45.0%
TTGTGACTGTGCCTGAGATT+GGG - tig0025571:5237-5256 None:intergenic 45.0%
ATTTGTCCGATCCACATGGT+AGG + tig0025571:5672-5691 Msa1423660:CDS 45.0%
TGCTGTTGCAGCAAATAACC+AGG - tig0025571:5725-5744 None:intergenic 45.0%
! CAAATTGTTGTTGCTGCTGC+TGG - tig0025571:5757-5776 None:intergenic 45.0%
CAGCAGCAGCAGTTTATTCA+TGG + tig0025571:5869-5888 Msa1423660:CDS 45.0%
GCAGCAGCAGTTTATTCATG+GGG + tig0025571:5871-5890 Msa1423660:CDS 45.0%
CAGCAGCAGTTTATTCATGG+GGG + tig0025571:5872-5891 Msa1423660:CDS 45.0%
TTCTGTGCAGCAGGCTAATA+TGG + tig0025571:6015-6034 Msa1423660:CDS 45.0%
TCTGTGCAGCAGGCTAATAT+GGG + tig0025571:6016-6035 Msa1423660:CDS 45.0%
TCTGCTAGAAGGGATTGTTG+TGG - tig0025571:6050-6069 None:intergenic 45.0%
!!! GATTTTGGGGTCTGCTAGAA+GGG - tig0025571:6060-6079 None:intergenic 45.0%
!!! CCGTCATTTCAGTTTTGGGT+AGG - tig0025571:6143-6162 None:intergenic 45.0%
! GCAGCCGTCATTTCAGTTTT+GGG - tig0025571:6147-6166 None:intergenic 45.0%
! AGCAGCCGTCATTTCAGTTT+TGG - tig0025571:6148-6167 None:intergenic 45.0%
!! TGCTGTTGATGCTGACTTGT+AGG - tig0025571:6216-6235 None:intergenic 45.0%
!! TGGTACTGTGAAGGCATTGT+GGG - tig0025571:6363-6382 None:intergenic 45.0%
AGCTGCTACTATGATGCAAC+CGG + tig0025571:6392-6411 Msa1423660:CDS 45.0%
AGGGGAGGAATCAACGGAAT+CGG - tig0025571:4091-4110 None:intergenic 50.0%
TTCCCGCAACAACGACTCAT+GGG + tig0025571:4113-4132 Msa1423660:CDS 50.0%
CATCAGACGGAGTTTGGATG+TGG - tig0025571:4157-4176 None:intergenic 50.0%
ACTGCACATCAGACGGAGTT+TGG - tig0025571:4163-4182 None:intergenic 50.0%
CTCCGTCTGATGTGCAGTTA+CGG + tig0025571:4165-4184 Msa1423660:CDS 50.0%
AAAGCTCTCTGTTACGTCGG+CGG + tig0025571:4216-4235 Msa1423660:CDS 50.0%
GTAGGATCGAGAATTTGCGG+TGG - tig0025571:4513-4532 None:intergenic 50.0%
! CCAGGTTGAGAAACACCATC+AGG - tig0025571:4699-4718 None:intergenic 50.0%
! CCTGATGGTGTTTCTCAACC+TGG + tig0025571:4696-4715 Msa1423660:CDS 50.0%
GTCTCCATCATAGGCGAATC+AGG - tig0025571:4768-4787 None:intergenic 50.0%
GACTTCTTCGTCTCCGTCTT+TGG + tig0025571:4806-4825 Msa1423660:CDS 50.0%
GGAGGCAAATTCGCCAAAGA+CGG - tig0025571:4822-4841 None:intergenic 50.0%
AGGGTGCATGTTGAAGATGG+TGG + tig0025571:4846-4865 Msa1423660:CDS 50.0%
!! GTTGAAGATGGTGGTTCTGG+TGG + tig0025571:4855-4874 Msa1423660:CDS 50.0%
!! ATGGTGGTTCTGGTGGTGTT+AGG + tig0025571:4862-4881 Msa1423660:CDS 50.0%
!! TGGTGGTTCTGGTGGTGTTA+GGG + tig0025571:4863-4882 Msa1423660:CDS 50.0%
! GCAGCAGGATCAGAAGGTTT+TGG + tig0025571:4920-4939 Msa1423660:CDS 50.0%
!! GTGTTGGTGGTATTGGACAG+AGG + tig0025571:4976-4995 Msa1423660:CDS 50.0%
! TGGTATTGGACAGAGGCTAC+AGG + tig0025571:4983-5002 Msa1423660:CDS 50.0%
ACTTGAACAACCTGTGGCTG+AGG - tig0025571:5185-5204 None:intergenic 50.0%
CTTGTGACTGTGCCTGAGAT+TGG - tig0025571:5238-5257 None:intergenic 50.0%
CAACAAAAGTCAAGTGGCGG+TGG + tig0025571:5287-5306 Msa1423660:CDS 50.0%
TCAACCTGGAACTACAAGGG+TGG + tig0025571:5637-5656 Msa1423660:CDS 50.0%
GTATCCACCCTTGTAGTTCC+AGG - tig0025571:5644-5663 None:intergenic 50.0%
CTGGATCCTACCATGTGGAT+CGG - tig0025571:5681-5700 None:intergenic 50.0%
CAGCAACATGTACAGGATCC+TGG + tig0025571:5704-5723 Msa1423660:CDS 50.0%
TGTTGAGGTTGTGGTTGCTG+CGG - tig0025571:5815-5834 None:intergenic 50.0%
TGAAGCTGTTGTTGCTGCTG+CGG - tig0025571:5842-5861 None:intergenic 50.0%
!!! GGATTTTGGGGTCTGCTAGA+AGG - tig0025571:6061-6080 None:intergenic 50.0%
! TGGTGGGGTTGGAGGATTTT+GGG - tig0025571:6074-6093 None:intergenic 50.0%
! TTGAACCAATGTGGTGGGGT+TGG - tig0025571:6085-6104 None:intergenic 50.0%
!! GTAGGGGAGCACTTCTAATG+GGG - tig0025571:6125-6144 None:intergenic 50.0%
!! GGTAGGGGAGCACTTCTAAT+GGG - tig0025571:6126-6145 None:intergenic 50.0%
! TTAGGAGCCATGGACTGAGA+CGG - tig0025571:6267-6286 None:intergenic 50.0%
!! CTGGTACTGTGAAGGCATTG+TGG - tig0025571:6364-6383 None:intergenic 50.0%
CATCATAGTAGCAGCTGCCA+TGG - tig0025571:6388-6407 None:intergenic 50.0%
! GATAACGGTGGTCCGGTTGA+CGG - tig0025571:4033-4052 None:intergenic 55.0%
!! TAGTTGAGGTGTGGTGGTGG+TGG - tig0025571:4066-4085 None:intergenic 55.0%
GAATCGGGGTAGTTGAGGTG+TGG - tig0025571:4075-4094 None:intergenic 55.0%
CAACGGAATCGGGGTAGTTG+AGG - tig0025571:4080-4099 None:intergenic 55.0%
GGGAGGAATCAACGGAATCG+GGG - tig0025571:4089-4108 None:intergenic 55.0%
GGGGAGGAATCAACGGAATC+GGG - tig0025571:4090-4109 None:intergenic 55.0%
GGAACGAGGGGAGGAATCAA+CGG - tig0025571:4097-4116 None:intergenic 55.0%
AGTCGTTGTTGCGGGAACGA+GGG - tig0025571:4110-4129 None:intergenic 55.0%
GTTCCCGCAACAACGACTCA+TGG + tig0025571:4112-4131 Msa1423660:CDS 55.0%
GTCCCATGAGTCGTTGTTGC+GGG - tig0025571:4118-4137 None:intergenic 55.0%
CGTCCCATGAGTCGTTGTTG+CGG - tig0025571:4119-4138 None:intergenic 55.0%
TTGGATGTGGCTGGAGGGAA+AGG - tig0025571:4144-4163 None:intergenic 55.0%
GGAGTTTGGATGTGGCTGGA+GGG - tig0025571:4149-4168 None:intergenic 55.0%
AGACGGAGTTTGGATGTGGC+TGG - tig0025571:4153-4172 None:intergenic 55.0%
CGCCGTAACTGCACATCAGA+CGG - tig0025571:4170-4189 None:intergenic 55.0%
CGTCTGATGTGCAGTTACGG+CGG + tig0025571:4168-4187 Msa1423660:CDS 55.0%
! GAGCTTTGTCGGTTGGACGA+GGG - tig0025571:4203-4222 None:intergenic 55.0%
! AGAGCTTTGTCGGTTGGACG+AGG - tig0025571:4204-4223 None:intergenic 55.0%
GGATAAATCCGCGAGTGAGG+TGG - tig0025571:4271-4290 None:intergenic 55.0%
CGTGGATAAATCCGCGAGTG+AGG - tig0025571:4274-4293 None:intergenic 55.0%
CGACGAAACGGCACCGTTTA+TGG - tig0025571:4565-4584 None:intergenic 55.0%
GCCGTTTCGTCGCTTAATCG+AGG + tig0025571:4573-4592 Msa1423660:CDS 55.0%
TCCACTGGAGAAGCTCCTGA+TGG + tig0025571:4681-4700 Msa1423660:CDS 55.0%
ACCATCAGGAGCTTCTCCAG+TGG - tig0025571:4685-4704 None:intergenic 55.0%
TGGACAGAGGCTACAGGATG+AGG + tig0025571:4989-5008 Msa1423660:CDS 55.0%
GGACAGAGGCTACAGGATGA+GGG + tig0025571:4990-5009 Msa1423660:CDS 55.0%
! GTAGTTGGCACTGGAGGATG+AGG - tig0025571:5038-5057 None:intergenic 55.0%
ATCGGATCATGGTGTGCCTG+TGG + tig0025571:5139-5158 Msa1423660:CDS 55.0%
TCGGATCATGGTGTGCCTGT+GGG + tig0025571:5140-5159 Msa1423660:CDS 55.0%
ACAACCTGTGGCTGAGGAGT+TGG - tig0025571:5179-5198 None:intergenic 55.0%
TCAAGCTCAGGCCCAATCTC+AGG + tig0025571:5223-5242 Msa1423660:CDS 55.0%
AAAGTCAAGTGGCGGTGGCT+CGG + tig0025571:5292-5311 Msa1423660:CDS 55.0%
CGATCCACATGGTAGGATCC+AGG + tig0025571:5679-5698 Msa1423660:CDS 55.0%
TGCACCTGGATCCTACCATG+TGG - tig0025571:5686-5705 None:intergenic 55.0%
CCTGTACATGTTGCTGCACC+TGG - tig0025571:5700-5719 None:intergenic 55.0%
CCAGGTGCAGCAACATGTAC+AGG + tig0025571:5697-5716 Msa1423660:CDS 55.0%
GAGGCTGTTGTTGCTGCTGA+TGG - tig0025571:5784-5803 None:intergenic 55.0%
TGCGGCTGATGTTGAGGTTG+TGG - tig0025571:5824-5843 None:intergenic 55.0%
TGATGGGGCTGTTGCTGTGA+TGG - tig0025571:5947-5966 None:intergenic 55.0%
! GGTGGGGTTGGAGGATTTTG+GGG - tig0025571:6073-6092 None:intergenic 55.0%
!! GTGGTGGGGTTGGAGGATTT+TGG - tig0025571:6075-6094 None:intergenic 55.0%
ATCCTCCAACCCCACCACAT+TGG + tig0025571:6077-6096 Msa1423660:intron 55.0%
! AACCAATGTGGTGGGGTTGG+AGG - tig0025571:6082-6101 None:intergenic 55.0%
!! GGGTAGGGGAGCACTTCTAA+TGG - tig0025571:6127-6146 None:intergenic 55.0%
GCTGCTTACAGAGCTGCAAC+TGG + tig0025571:6165-6184 Msa1423660:CDS 55.0%
GCTTACAGAGCTGCAACTGG+AGG + tig0025571:6168-6187 Msa1423660:CDS 55.0%
GGCAGCAGAATTAGGAGCCA+TGG - tig0025571:6277-6296 None:intergenic 55.0%
GCCTTCACAGTACCAGACCA+TGG + tig0025571:6368-6387 Msa1423660:CDS 55.0%
GCCATGGTCTGGTACTGTGA+AGG - tig0025571:6372-6391 None:intergenic 55.0%
TAGTAGCAGCTGCCATGGTC+TGG - tig0025571:6383-6402 None:intergenic 55.0%
GGATGCTGTGCAGGAACTTC+CGG - tig0025571:6414-6433 None:intergenic 55.0%
! TGCTGCTTCATGCCGTCTGA+CGG - tig0025571:6435-6454 None:intergenic 55.0%
! GTTGACGGCGTTGGAGGTTG+GGG - tig0025571:4018-4037 None:intergenic 60.0%
! GGTTGACGGCGTTGGAGGTT+GGG - tig0025571:4019-4038 None:intergenic 60.0%
CAACCTCCAACGCCGTCAAC+CGG + tig0025571:4018-4037 Msa1423660:CDS 60.0%
! CACGGAGGATAACGGTGGTC+CGG - tig0025571:4040-4059 None:intergenic 60.0%
GGTGGAGCCACGGAGGATAA+CGG - tig0025571:4048-4067 None:intergenic 60.0%
GGGTAGTTGAGGTGTGGTGG+TGG - tig0025571:4069-4088 None:intergenic 60.0%
TCGGGGTAGTTGAGGTGTGG+TGG - tig0025571:4072-4091 None:intergenic 60.0%
GTCGTTGTTGCGGGAACGAG+GGG - tig0025571:4109-4128 None:intergenic 60.0%
GAGTCGTTGTTGCGGGAACG+AGG - tig0025571:4111-4130 None:intergenic 60.0%
CGGAGTTTGGATGTGGCTGG+AGG - tig0025571:4150-4169 None:intergenic 60.0%
!!! CAAGGTTTTGGAGAGGCGCG+TGG - tig0025571:4292-4311 None:intergenic 60.0%
GCAGCAGCAGCAGGATCAGA+AGG + tig0025571:4914-4933 Msa1423660:CDS 60.0%
! GTTGGCACTGGAGGATGAGG+CGG - tig0025571:5035-5054 None:intergenic 60.0%
TCCTCCAGTGCCAACTACGC+TGG + tig0025571:5040-5059 Msa1423660:CDS 60.0%
TCCAGTGCCAACTACGCTGG+CGG + tig0025571:5043-5062 Msa1423660:CDS 60.0%
! GCGGCGGTTGGTGTTCCAAT+TGG + tig0025571:5062-5081 Msa1423660:CDS 60.0%
ACCACCAACTCCTCAGCCAC+AGG + tig0025571:5172-5191 Msa1423660:CDS 60.0%
ACCTGTGGCTGAGGAGTTGG+TGG - tig0025571:5176-5195 None:intergenic 60.0%
GGTTGCTGCGGCTGATGTTG+AGG - tig0025571:5803-5822 None:intergenic 60.0%
TGCTGCTGCGGCTGATGTTG+AGG - tig0025571:5830-5849 None:intergenic 60.0%
ACAGCAACAGCCCCATCACC+AGG + tig0025571:5949-5968 Msa1423660:CDS 60.0%
CCTTGTTGGGGCTGTCTTGC+AGG - tig0025571:5986-6005 None:intergenic 60.0%
CCTGCAAGACAGCCCCAACA+AGG + tig0025571:5983-6002 Msa1423660:CDS 60.0%
! CCATGGACTGAGACGGATGG+TGG - tig0025571:6260-6279 None:intergenic 60.0%
CCACCATCCGTCTCAGTCCA+TGG + tig0025571:6257-6276 Msa1423660:CDS 60.0%
! GAGCCATGGACTGAGACGGA+TGG - tig0025571:6263-6282 None:intergenic 60.0%
CTGGCAGGGGCAGCAGAATT+AGG - tig0025571:6285-6304 None:intergenic 60.0%
!! GAAGGCATTGTGGGCGCCAT+AGG - tig0025571:6354-6373 None:intergenic 60.0%
CCTGCACAGCATCCGTCAGA+CGG + tig0025571:6420-6439 Msa1423660:CDS 60.0%
! CGGTTGACGGCGTTGGAGGT+TGG - tig0025571:4020-4039 None:intergenic 65.0%
CGGACCACCGTTATCCTCCG+TGG + tig0025571:4038-4057 Msa1423660:CDS 65.0%
! GGAGCCACGGAGGATAACGG+TGG - tig0025571:4045-4064 None:intergenic 65.0%
!! GTGTGGTGGTGGTGGAGCCA+CGG - tig0025571:4058-4077 None:intergenic 65.0%
GTTGTTGCGGGAACGAGGGG+AGG - tig0025571:4106-4125 None:intergenic 65.0%
CGGTTGGACGAGGGACGATG+TGG - tig0025571:4194-4213 None:intergenic 65.0%
TGAACGCGCCACCTCACTCG+CGG + tig0025571:4260-4279 Msa1423660:CDS 65.0%
GCAACAGCAGCAGCAGCAGC+AGG + tig0025571:4905-4924 Msa1423660:CDS 65.0%
! GCCAGCGTAGTTGGCACTGG+AGG - tig0025571:5044-5063 None:intergenic 65.0%
AGTGCCAACTACGCTGGCGG+CGG + tig0025571:5046-5065 Msa1423660:CDS 65.0%
! CCGTCTGACGGATGCTGTGC+AGG - tig0025571:6423-6442 None:intergenic 65.0%
! GGTCCGGTTGACGGCGTTGG+AGG - tig0025571:4024-4043 None:intergenic 70.0%
GGTGGTCCGGTTGACGGCGT+TGG - tig0025571:4027-4046 None:intergenic 70.0%
!! TGGTGGTGGTGGAGCCACGG+AGG - tig0025571:4055-4074 None:intergenic 70.0%
GCCGCCAGCGTAGTTGGCAC+TGG - tig0025571:5047-5066 None:intergenic 70.0%
CCAACCGCCGCCAGCGTAGT+TGG - tig0025571:5053-5072 None:intergenic 70.0%
CCAACTACGCTGGCGGCGGT+TGG + tig0025571:5050-5069 Msa1423660:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0025571 gene 3887 6479 3887 ID=Msa1423660;Name=Msa1423660
tig0025571 mRNA 3887 6479 3887 ID=Msa1423660-mRNA-1;Parent=Msa1423660;Name=Msa1423660-mRNA-1;_AED=0.10;_eAED=0.10;_QI=116|0.5|0.33|1|1|1|3|0|723
tig0025571 exon 3887 5342 3887 ID=Msa1423660-mRNA-1:exon:3355;Parent=Msa1423660-mRNA-1
tig0025571 exon 5571 6037 5571 ID=Msa1423660-mRNA-1:exon:3356;Parent=Msa1423660-mRNA-1
tig0025571 exon 6115 6479 6115 ID=Msa1423660-mRNA-1:exon:3357;Parent=Msa1423660-mRNA-1
tig0025571 five_prime_UTR 3887 4002 3887 ID=Msa1423660-mRNA-1:five_prime_utr;Parent=Msa1423660-mRNA-1
tig0025571 CDS 4003 5342 4003 ID=Msa1423660-mRNA-1:cds;Parent=Msa1423660-mRNA-1
tig0025571 CDS 5571 6037 5571 ID=Msa1423660-mRNA-1:cds;Parent=Msa1423660-mRNA-1
tig0025571 CDS 6115 6479 6115 ID=Msa1423660-mRNA-1:cds;Parent=Msa1423660-mRNA-1
Gene Sequence

>Msa1423660

ATGGAGGCGCAACCCCAACCTCCAACGCCGTCAACCGGACCACCGTTATCCTCCGTGGCTCCACCACCACCACACCTCAACTACCCCGATTCCGTTGATTCCTCCCCTCGTTCCCGCAACAACGACTCATGGGACGAACCTTTCCCTCCAGCCACATCCAAACTCCGTCTGATGTGCAGTTACGGCGGCCACATCGTCCCTCGTCCAACCGACAAAGCTCTCTGTTACGTCGGCGGTGACACTCGCATCGTTGTTGTTGAACGCGCCACCTCACTCGCGGATTTATCCACGCGCCTCTCCAAAACCTTGCTTAATGGAAGACCTTTCACTTTCAAGTATCAGCTTCCAAACGAAGACCTAGATTCGTTAATCTCCGTTTCAACCGATGAAGATTTAGAAAATATGATCGATGAATACGATCGAACGAATTCTACGAATTCGTCTGTGAAACCCTCGCGAATTCGTCTTTTTCTGTTTCCATCGAAACCTGAATCTGCTCAGTCAATTCCACCGCAAATTCTCGATCCTACTGTTAGATCCGGTGAATGGTTCTTTGATACCATAAACGGTGCCGTTTCGTCGCTTAATCGAGGTTTTTCTGATTCTGCTGCTTCTTCTGTGAATCATCTTTTAGGTCTTGATGATGATGTTGCTGGGAATAATCATATTGAACAAGGTTCCACTGGAGAAGCTCCTGATGGTGTTTCTCAACCTGGATCTTTTGGGAATTGTAAGAATTTGAAGCAGGATGTTCATTCTGTTCCTGATTCGCCTATGATGGAGACTTCTTCGTCCTTTGGTTCGACTTCTTCGTCTCCGTCTTTGGCGAATTTGCCTCCTATTAGGGTGCATGTTGAAGATGGTGGTTCTGGTGGTGTTAGGGTTCAACAGCAGCAACAATTGCAACAGCAGCAGCAGCAGCAGGATCAGAAGGTTTTGGGGATTGATGAACAGTTTGCTCAAATGGTTGTTGGTGTTGGTGGTATTGGACAGAGGCTACAGGATGAGGGTTTTGCTGTCATGTCTTCACCGCCTCATCCTCCAGTGCCAACTACGCTGGCGGCGGTTGGTGTTCCAATTGGTTCTGCTGTGGTTGTTGGTGATTACCAGAATAGGGTATTCTCTGATGATGAGAGATCGGATCATGGTGTGCCTGTGGGATATAGAAAACCACCAACTCCTCAGCCACAGGTTGTTCAAGTTCAACCTCAAGCTCAAAATCAAGCTCAGGCCCAATCTCAGGCACAGTCACAAGCACAGACACAGTCACAAGCTCCTCAATTTCAACAAAAGTCAAGTGGCGGTGGCTCGGATTTGCCTTCTCCTGATTCAGTTTCAAGTGATAGTAGTCTTTCAAATGCGATGTCACGTCAAAAACCTGTTGTTTATCAAGAGCAAATTCAAATTCAACCTGGAACTACAAGGGTGGATACGAAAATCAATTTGTCCGATCCACATGGTAGGATCCAGGTGCAGCAACATGTACAGGATCCTGGTTATTTGCTGCAACAGCAATTTGAACTCCAGCAGCAGCAACAACAATTTGAACTCCATCAGCAGCAACAACAGCCTCAACATCAGCCGCAGCAACCACAACCTCAACATCAGCCGCAGCAGCAACAACAGCTTCAACATCATCAGCAGCAGCAGTTTATTCATGGGGGACACTACATTCATCATAACCCTGCAATTCCTACATACTATCCTGTATATCCATCACAGCAACAGCCCCATCACCAGGTTTACTATGTACCTGCAAGACAGCCCCAACAAGGTTACAATATTTCTGTGCAGCAGGCTAATATGGCTTACAACCCCATTAGAAGTGCTCCCCTACCCAAAACTGAAATGACGGCTGCTGCTTACAGAGCTGCAACTGGAGGCAGTCCTCAATTTGTGCAAGTTCCTACAAGTCAGCATCAACAGCAGTATGTCACTTACTCTCAGATCCACCATCCGTCTCAGTCCATGGCTCCTAATTCTGCTGCCCCTGCCAGTTATGCTTATGAATATGCTGATCCTGCTCAAGTATACTACTCTCAGCCTATGGCGCCCACAATGCCTTCACAGTACCAGACCATGGCAGCTGCTACTATGATGCAACCGGAAGTTCCTGCACAGCATCCGTCAGACGGCATGAAGCAGCAGATAAGAACCTCACAGCCACTATAA
Protein sequence

>Msa1423660

MEAQPQPPTPSTGPPLSSVAPPPPHLNYPDSVDSSPRSRNNDSWDEPFPPATSKLRLMCSYGGHIVPRPTDKALCYVGGDTRIVVVERATSLADLSTRLSKTLLNGRPFTFKYQLPNEDLDSLISVSTDEDLENMIDEYDRTNSTNSSVKPSRIRLFLFPSKPESAQSIPPQILDPTVRSGEWFFDTINGAVSSLNRGFSDSAASSVNHLLGLDDDVAGNNHIEQGSTGEAPDGVSQPGSFGNCKNLKQDVHSVPDSPMMETSSSFGSTSSSPSLANLPPIRVHVEDGGSGGVRVQQQQQLQQQQQQQDQKVLGIDEQFAQMVVGVGGIGQRLQDEGFAVMSSPPHPPVPTTLAAVGVPIGSAVVVGDYQNRVFSDDERSDHGVPVGYRKPPTPQPQVVQVQPQAQNQAQAQSQAQSQAQTQSQAPQFQQKSSGGGSDLPSPDSVSSDSSLSNAMSRQKPVVYQEQIQIQPGTTRVDTKINLSDPHGRIQVQQHVQDPGYLLQQQFELQQQQQQFELHQQQQQPQHQPQQPQPQHQPQQQQQLQHHQQQQFIHGGHYIHHNPAIPTYYPVYPSQQQPHHQVYYVPARQPQQGYNISVQQANMAYNPIRSAPLPKTEMTAAAYRAATGGSPQFVQVPTSQHQQQYVTYSQIHHPSQSMAPNSAAPASYAYEYADPAQVYYSQPMAPTMPSQYQTMAAATMMQPEVPAQHPSDGMKQQIRTSQPL*