Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0274710 | XP_003593973.1 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 4.07e-68 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0274710 | sp|Q9LIF1|GRS10_ARATH | 75.490 | 102 | 25 | 0 | 1 | 102 | 1 | 102 | 3.23e-56 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0274710 | G7ISG7 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 1.95e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0274710 | Msa0374300 | 0.811930 | 5.471944e-51 | -8.615850e-47 |
| Msa0274710 | Msa0439950 | 0.991530 | 3.880918e-188 | -8.615850e-47 |
| Msa0274710 | Msa0469700 | 0.815337 | 9.734308e-52 | -8.615850e-47 |
| Msa0274710 | Msa0515530 | 0.814047 | 1.879620e-51 | -8.615850e-47 |
| Msa0274710 | Msa0515580 | 0.812825 | 3.488857e-51 | -8.615850e-47 |
| Msa0274710 | Msa0931410 | 0.812931 | 3.307044e-51 | -8.615850e-47 |
| Msa0274710 | Msa0974470 | 0.805086 | 1.581625e-49 | -8.615850e-47 |
| Msa0274710 | Msa1365030 | 0.816496 | 5.366838e-52 | -8.615850e-47 |
| Msa0274710 | Msa1367400 | 0.805738 | 1.154595e-49 | -8.615850e-47 |
| Msa0274710 | Msa1462680 | 0.806827 | 6.808288e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0274710 | MtrunA17_Chr2g0286271 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 3.75e-72 | 209 |
| Msa0274710 | MtrunA17_Chr7g0268731 | 64.078 | 103 | 36 | 1 | 1 | 102 | 1 | 103 | 6.59e-46 | 143 |
| Msa0274710 | MtrunA17_Chr7g0268721 | 63.107 | 103 | 37 | 1 | 1 | 102 | 1 | 103 | 4.39e-45 | 141 |
| Msa0274710 | MtrunA17_Chr2g0286251 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 1.93e-44 | 139 |
| Msa0274710 | MtrunA17_Chr1g0195971 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 2.31e-44 | 139 |
| Msa0274710 | MtrunA17_Chr4g0066791 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0274710 | MtrunA17_Chr4g0066801 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0274710 | MtrunA17_Chr4g0066811 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0274710 | MtrunA17_Chr7g0224091 | 60.194 | 103 | 40 | 1 | 1 | 102 | 22 | 124 | 1.06e-39 | 128 |
| Msa0274710 | MtrunA17_Chr7g0268711 | 54.737 | 95 | 43 | 0 | 1 | 95 | 1 | 95 | 1.29e-36 | 119 |
| Msa0274710 | MtrunA17_Chr1g0195991 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 2.44e-36 | 119 |
| Msa0274710 | MtrunA17_Chr1g0195981 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 2.97e-36 | 118 |
| Msa0274710 | MtrunA17_Chr7g0222541 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 5.73e-35 | 116 |
| Msa0274710 | MtrunA17_Chr7g0222551 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 1.13e-34 | 115 |
| Msa0274710 | MtrunA17_Chr7g0222491 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 1.69e-34 | 115 |
| Msa0274710 | MtrunA17_Chr1g0195951 | 52.941 | 102 | 48 | 0 | 1 | 102 | 1 | 102 | 5.75e-34 | 112 |
| Msa0274710 | MtrunA17_Chr7g0268751 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 2.04e-32 | 108 |
| Msa0274710 | MtrunA17_Chr2g0303741 | 47.619 | 105 | 51 | 1 | 2 | 102 | 40 | 144 | 1.56e-26 | 95.5 |
| Msa0274710 | MtrunA17_Chr7g0268761 | 40.196 | 102 | 61 | 0 | 1 | 102 | 1 | 102 | 1.20e-24 | 89.4 |
| Msa0274710 | MtrunA17_Chr1g0195931 | 41.176 | 102 | 60 | 0 | 1 | 102 | 1 | 102 | 9.52e-24 | 87.4 |
| Msa0274710 | MtrunA17_Chr2g0325001 | 40.777 | 103 | 54 | 2 | 4 | 102 | 41 | 140 | 1.22e-20 | 80.5 |
| Msa0274710 | MtrunA17_Chr4g0040601 | 41.837 | 98 | 56 | 1 | 6 | 102 | 58 | 155 | 2.34e-20 | 80.1 |
| Msa0274710 | MtrunA17_Chr2g0282951 | 34.653 | 101 | 66 | 0 | 2 | 102 | 27 | 127 | 9.79e-20 | 77.8 |
| Msa0274710 | MtrunA17_Chr5g0434201 | 40.777 | 103 | 59 | 1 | 2 | 102 | 21 | 123 | 7.16e-18 | 72.8 |
| Msa0274710 | MtrunA17_Chr3g0117411 | 34.314 | 102 | 67 | 0 | 1 | 102 | 18 | 119 | 4.49e-17 | 70.5 |
| Msa0274710 | MtrunA17_Chr8g0376641 | 39.806 | 103 | 60 | 1 | 2 | 102 | 21 | 123 | 1.35e-16 | 69.3 |
| Msa0274710 | MtrunA17_Chr1g0151231 | 37.736 | 106 | 56 | 3 | 3 | 102 | 48 | 149 | 4.37e-16 | 68.9 |
| Msa0274710 | MtrunA17_Chr3g0135811 | 35.000 | 100 | 59 | 2 | 3 | 101 | 23 | 117 | 2.22e-15 | 66.2 |
| Msa0274710 | MtrunA17_Chr7g0228311 | 35.000 | 100 | 65 | 0 | 1 | 100 | 3 | 102 | 4.10e-15 | 65.1 |
| Msa0274710 | MtrunA17_Chr3g0117391 | 33.000 | 100 | 67 | 0 | 1 | 100 | 21 | 120 | 5.83e-15 | 65.5 |
| Msa0274710 | MtrunA17_Chr1g0182971 | 35.354 | 99 | 63 | 1 | 2 | 99 | 84 | 182 | 6.20e-14 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0274710 | AT3G21460.1 | 75.490 | 102 | 25 | 0 | 1 | 102 | 1 | 102 | 3.28e-57 | 172 |
| Msa0274710 | AT4G15680.1 | 63.725 | 102 | 37 | 0 | 1 | 102 | 1 | 102 | 1.51e-44 | 140 |
| Msa0274710 | AT4G15700.1 | 63.725 | 102 | 37 | 0 | 1 | 102 | 1 | 102 | 3.88e-44 | 139 |
| Msa0274710 | AT4G15660.1 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 6.21e-44 | 138 |
| Msa0274710 | AT4G15670.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 9.32e-44 | 138 |
| Msa0274710 | AT5G18600.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 2.10e-43 | 137 |
| Msa0274710 | AT4G15690.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 1.96e-42 | 134 |
| Msa0274710 | AT2G47870.1 | 58.252 | 103 | 42 | 1 | 1 | 102 | 1 | 103 | 1.06e-41 | 132 |
| Msa0274710 | AT3G62950.1 | 60.194 | 103 | 40 | 1 | 1 | 102 | 1 | 103 | 1.52e-41 | 132 |
| Msa0274710 | AT2G30540.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 4.76e-35 | 115 |
| Msa0274710 | AT1G03020.1 | 52.941 | 102 | 48 | 0 | 1 | 102 | 1 | 102 | 1.64e-34 | 114 |
| Msa0274710 | AT3G62930.1 | 51.961 | 102 | 49 | 0 | 1 | 102 | 1 | 102 | 2.00e-34 | 114 |
| Msa0274710 | AT1G06830.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 9.36e-34 | 112 |
| Msa0274710 | AT2G47880.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 5.76e-33 | 110 |
| Msa0274710 | AT3G62960.1 | 49.474 | 95 | 48 | 0 | 1 | 95 | 1 | 95 | 8.89e-32 | 107 |
| Msa0274710 | AT5G14070.1 | 50.000 | 110 | 47 | 2 | 1 | 102 | 31 | 140 | 6.88e-30 | 104 |
| Msa0274710 | AT3G02000.1 | 51.887 | 106 | 45 | 1 | 3 | 102 | 31 | 136 | 2.47e-27 | 97.4 |
| Msa0274710 | AT1G28480.1 | 33.333 | 105 | 66 | 2 | 2 | 102 | 33 | 137 | 8.78e-19 | 75.9 |
| Msa0274710 | AT4G33040.1 | 40.187 | 107 | 54 | 3 | 3 | 102 | 41 | 144 | 8.12e-17 | 70.9 |
| Msa0274710 | AT1G03850.2 | 35.484 | 93 | 56 | 1 | 10 | 102 | 62 | 150 | 3.51e-15 | 67.0 |
| Msa0274710 | AT5G11930.1 | 37.000 | 100 | 53 | 3 | 3 | 101 | 57 | 147 | 6.83e-15 | 66.2 |
| Msa0274710 | AT5G63030.1 | 34.000 | 100 | 66 | 0 | 1 | 100 | 19 | 118 | 2.44e-14 | 63.9 |
| Msa0274710 | AT5G40370.1 | 32.000 | 100 | 68 | 0 | 1 | 100 | 3 | 102 | 1.24e-13 | 62.0 |
| Msa0274710 | AT4G28730.1 | 35.714 | 98 | 62 | 1 | 2 | 98 | 71 | 168 | 1.44e-13 | 63.2 |
| Msa0274710 | AT2G20270.1 | 32.990 | 97 | 64 | 1 | 2 | 97 | 76 | 172 | 7.28e-13 | 61.6 |
| Msa0274710 | AT5G20500.1 | 35.052 | 97 | 63 | 0 | 2 | 98 | 33 | 129 | 2.09e-11 | 56.6 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGATCGCATATCGAAAT+TGG | 0.300555 | 2_3:+6579049 | None:intergenic |
| TTAGAGAAGCTGGTGCTCTT+TGG | 0.348544 | 2_3:+6579330 | Msa0274710:CDS |
| ATCGAAATTGGCATCGCAAA+AGG | 0.406599 | 2_3:+6579061 | Msa0274710:CDS |
| AATGAACACTGCTGGAACGC+TGG | 0.408792 | 2_3:-6579237 | None:intergenic |
| AGCGTTCCAGCAGTGTTCAT+TGG | 0.414107 | 2_3:+6579239 | Msa0274710:CDS |
| TCATCTAGCTCATAGATTGC+TGG | 0.425719 | 2_3:-6579161 | None:intergenic |
| ATGAACACTGCTGGAACGCT+GGG | 0.430415 | 2_3:-6579236 | None:intergenic |
| TTCATTGGTGGCAAGTTTGT+GGG | 0.440094 | 2_3:+6579254 | Msa0274710:CDS |
| GTTCATTGGTGGCAAGTTTG+TGG | 0.450468 | 2_3:+6579253 | Msa0274710:CDS |
| AAGAAAATGCTTAGAGAAGC+TGG | 0.460268 | 2_3:+6579320 | Msa0274710:CDS |
| ATGAGCTAGATGAAGATAAG+AGG | 0.471120 | 2_3:+6579171 | Msa0274710:CDS |
| TGAGTGAGCCATTGAGATGA+AGG | 0.478669 | 2_3:-6579298 | None:intergenic |
| TTGCCACCAATGAACACTGC+TGG | 0.487093 | 2_3:-6579245 | None:intergenic |
| GAATGGGCTCTTATAAGATT+AGG | 0.493326 | 2_3:+6579206 | Msa0274710:CDS |
| ATCATGACCCTTCATCTCAA+TGG | 0.503503 | 2_3:+6579290 | Msa0274710:CDS |
| GGATGAGCCATGCAATTAAG+AGG | 0.507985 | 2_3:+6579114 | Msa0274710:CDS |
| ATCTTTAGCAAGAGTTCATG+TGG | 0.520709 | 2_3:+6579092 | Msa0274710:CDS |
| TGAGCTAGATGAAGATAAGA+GGG | 0.550399 | 2_3:+6579172 | Msa0274710:CDS |
| AAAATAGCCTCTTAATTGCA+TGG | 0.552086 | 2_3:-6579121 | None:intergenic |
| GTTCCAGCAGTGTTCATTGG+TGG | 0.567163 | 2_3:+6579242 | Msa0274710:CDS |
| AGCTAGATGAAGATAAGAGG+GGG | 0.570466 | 2_3:+6579174 | Msa0274710:CDS |
| GAAATTGGCATCGCAAAAGG+CGG | 0.634320 | 2_3:+6579064 | Msa0274710:CDS |
| TCTTTAGCAAGAGTTCATGT+GGG | 0.649738 | 2_3:+6579093 | Msa0274710:CDS |
| ATGAAGGGTCATGATTATGT+TGG | 0.653275 | 2_3:-6579282 | None:intergenic |
| GAGTGAGCCATTGAGATGAA+GGG | 0.664431 | 2_3:-6579297 | None:intergenic |
| GAGCTAGATGAAGATAAGAG+GGG | 0.665707 | 2_3:+6579173 | Msa0274710:CDS |
| TGAACACTGCTGGAACGCTG+GGG | 0.703546 | 2_3:-6579235 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAGCCTCTTAATTGCA+TGG | - | chr2_3:6579124-6579143 | None:intergenic | 30.0% | |
| ATCTTTAGCAAGAGTTCATG+TGG | + | chr2_3:6579092-6579111 | Msa0274710:CDS | 35.0% | |
| TCTTTAGCAAGAGTTCATGT+GGG | + | chr2_3:6579093-6579112 | Msa0274710:CDS | 35.0% | |
| !! | AAGAGGCTATTTTATGAGCA+AGG | + | chr2_3:6579131-6579150 | Msa0274710:CDS | 35.0% |
| ! | CTATTTTATGAGCAAGGTGT+TGG | + | chr2_3:6579137-6579156 | Msa0274710:CDS | 35.0% |
| ATGAGCTAGATGAAGATAAG+AGG | + | chr2_3:6579171-6579190 | Msa0274710:CDS | 35.0% | |
| TGAGCTAGATGAAGATAAGA+GGG | + | chr2_3:6579172-6579191 | Msa0274710:CDS | 35.0% | |
| ! | GAATGGGCTCTTATAAGATT+AGG | + | chr2_3:6579206-6579225 | Msa0274710:CDS | 35.0% |
| ATGAAGGGTCATGATTATGT+TGG | - | chr2_3:6579285-6579304 | None:intergenic | 35.0% | |
| AAGAAAATGCTTAGAGAAGC+TGG | + | chr2_3:6579320-6579339 | Msa0274710:CDS | 35.0% | |
| ATCGAAATTGGCATCGCAAA+AGG | + | chr2_3:6579061-6579080 | Msa0274710:CDS | 40.0% | |
| ! | TCATCTAGCTCATAGATTGC+TGG | - | chr2_3:6579164-6579183 | None:intergenic | 40.0% |
| GAGCTAGATGAAGATAAGAG+GGG | + | chr2_3:6579173-6579192 | Msa0274710:CDS | 40.0% | |
| AGCTAGATGAAGATAAGAGG+GGG | + | chr2_3:6579174-6579193 | Msa0274710:CDS | 40.0% | |
| ! | TTCATTGGTGGCAAGTTTGT+GGG | + | chr2_3:6579254-6579273 | Msa0274710:CDS | 40.0% |
| ATCATGACCCTTCATCTCAA+TGG | + | chr2_3:6579290-6579309 | Msa0274710:CDS | 40.0% | |
| GAAATTGGCATCGCAAAAGG+CGG | + | chr2_3:6579064-6579083 | Msa0274710:CDS | 45.0% | |
| GGATGAGCCATGCAATTAAG+AGG | + | chr2_3:6579114-6579133 | Msa0274710:CDS | 45.0% | |
| AGATGAAGATAAGAGGGGGA+AGG | + | chr2_3:6579178-6579197 | Msa0274710:CDS | 45.0% | |
| AGATAAGAGGGGGAAGGAAA+TGG | + | chr2_3:6579184-6579203 | Msa0274710:CDS | 45.0% | |
| ! | GTTCATTGGTGGCAAGTTTG+TGG | + | chr2_3:6579253-6579272 | Msa0274710:CDS | 45.0% |
| GAGTGAGCCATTGAGATGAA+GGG | - | chr2_3:6579300-6579319 | None:intergenic | 45.0% | |
| TGAGTGAGCCATTGAGATGA+AGG | - | chr2_3:6579301-6579320 | None:intergenic | 45.0% | |
| TTAGAGAAGCTGGTGCTCTT+TGG | + | chr2_3:6579330-6579349 | Msa0274710:CDS | 45.0% | |
| AGAGGGGGAAGGAAATGGAA+TGG | + | chr2_3:6579189-6579208 | Msa0274710:CDS | 50.0% | |
| GAGGGGGAAGGAAATGGAAT+GGG | + | chr2_3:6579190-6579209 | Msa0274710:CDS | 50.0% | |
| ATGAACACTGCTGGAACGCT+GGG | - | chr2_3:6579239-6579258 | None:intergenic | 50.0% | |
| AATGAACACTGCTGGAACGC+TGG | - | chr2_3:6579240-6579259 | None:intergenic | 50.0% | |
| AGCGTTCCAGCAGTGTTCAT+TGG | + | chr2_3:6579239-6579258 | Msa0274710:CDS | 50.0% | |
| GTTCCAGCAGTGTTCATTGG+TGG | + | chr2_3:6579242-6579261 | Msa0274710:CDS | 50.0% | |
| TTGCCACCAATGAACACTGC+TGG | - | chr2_3:6579248-6579267 | None:intergenic | 50.0% | |
| TGAACACTGCTGGAACGCTG+GGG | - | chr2_3:6579238-6579257 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 6579050 | 6579358 | 6579050 | ID=Msa0274710;Name=Msa0274710 |
| chr2_3 | mRNA | 6579050 | 6579358 | 6579050 | ID=Msa0274710-mRNA-1;Parent=Msa0274710;Name=Msa0274710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr2_3 | exon | 6579050 | 6579358 | 6579050 | ID=Msa0274710-mRNA-1:exon:3048;Parent=Msa0274710-mRNA-1 |
| chr2_3 | CDS | 6579050 | 6579358 | 6579050 | ID=Msa0274710-mRNA-1:cds;Parent=Msa0274710-mRNA-1 |
| Gene Sequence |
| Protein sequence |
