Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0439950 | XP_003593973.1 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 4.07e-68 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0439950 | sp|Q9LIF1|GRS10_ARATH | 75.490 | 102 | 25 | 0 | 1 | 102 | 1 | 102 | 3.23e-56 | 172 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0439950 | G7ISG7 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 1.95e-68 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0374300 | Msa0439950 | 0.808023 | 3.795778e-50 | -8.615850e-47 |
| Msa0439950 | Msa0469700 | 0.812032 | 5.198539e-51 | -8.615850e-47 |
| Msa0439950 | Msa0515530 | 0.810095 | 1.366456e-50 | -8.615850e-47 |
| Msa0439950 | Msa0515580 | 0.808966 | 2.388590e-50 | -8.615850e-47 |
| Msa0439950 | Msa0871840 | 0.800897 | 1.161763e-48 | -8.615850e-47 |
| Msa0439950 | Msa0931410 | 0.809537 | 1.801833e-50 | -8.615850e-47 |
| Msa0439950 | Msa0974470 | 0.807994 | 3.850679e-50 | -8.615850e-47 |
| Msa0439950 | Msa1365030 | 0.820703 | 5.962849e-53 | -8.615850e-47 |
| Msa0439950 | Msa1367400 | 0.811219 | 7.812494e-51 | -8.615850e-47 |
| Msa0439950 | Msa1462680 | 0.812314 | 4.512548e-51 | -8.615850e-47 |
| Msa0274710 | Msa0439950 | 0.991530 | 3.880918e-188 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0439950 | MtrunA17_Chr2g0286271 | 99.020 | 102 | 1 | 0 | 1 | 102 | 1 | 102 | 3.75e-72 | 209 |
| Msa0439950 | MtrunA17_Chr7g0268731 | 64.078 | 103 | 36 | 1 | 1 | 102 | 1 | 103 | 6.59e-46 | 143 |
| Msa0439950 | MtrunA17_Chr7g0268721 | 63.107 | 103 | 37 | 1 | 1 | 102 | 1 | 103 | 4.39e-45 | 141 |
| Msa0439950 | MtrunA17_Chr2g0286251 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 1.93e-44 | 139 |
| Msa0439950 | MtrunA17_Chr1g0195971 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 2.31e-44 | 139 |
| Msa0439950 | MtrunA17_Chr4g0066791 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0439950 | MtrunA17_Chr4g0066801 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0439950 | MtrunA17_Chr4g0066811 | 65.049 | 103 | 35 | 1 | 1 | 102 | 1 | 103 | 8.29e-44 | 137 |
| Msa0439950 | MtrunA17_Chr7g0224091 | 60.194 | 103 | 40 | 1 | 1 | 102 | 22 | 124 | 1.06e-39 | 128 |
| Msa0439950 | MtrunA17_Chr7g0268711 | 54.737 | 95 | 43 | 0 | 1 | 95 | 1 | 95 | 1.29e-36 | 119 |
| Msa0439950 | MtrunA17_Chr1g0195991 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 2.44e-36 | 119 |
| Msa0439950 | MtrunA17_Chr1g0195981 | 55.789 | 95 | 42 | 0 | 1 | 95 | 1 | 95 | 2.97e-36 | 118 |
| Msa0439950 | MtrunA17_Chr7g0222541 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 5.73e-35 | 116 |
| Msa0439950 | MtrunA17_Chr7g0222551 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 1.13e-34 | 115 |
| Msa0439950 | MtrunA17_Chr7g0222491 | 55.340 | 103 | 45 | 1 | 1 | 102 | 30 | 132 | 1.69e-34 | 115 |
| Msa0439950 | MtrunA17_Chr1g0195951 | 52.941 | 102 | 48 | 0 | 1 | 102 | 1 | 102 | 5.75e-34 | 112 |
| Msa0439950 | MtrunA17_Chr7g0268751 | 46.078 | 102 | 55 | 0 | 1 | 102 | 1 | 102 | 2.04e-32 | 108 |
| Msa0439950 | MtrunA17_Chr2g0303741 | 47.619 | 105 | 51 | 1 | 2 | 102 | 40 | 144 | 1.56e-26 | 95.5 |
| Msa0439950 | MtrunA17_Chr7g0268761 | 40.196 | 102 | 61 | 0 | 1 | 102 | 1 | 102 | 1.20e-24 | 89.4 |
| Msa0439950 | MtrunA17_Chr1g0195931 | 41.176 | 102 | 60 | 0 | 1 | 102 | 1 | 102 | 9.52e-24 | 87.4 |
| Msa0439950 | MtrunA17_Chr2g0325001 | 40.777 | 103 | 54 | 2 | 4 | 102 | 41 | 140 | 1.22e-20 | 80.5 |
| Msa0439950 | MtrunA17_Chr4g0040601 | 41.837 | 98 | 56 | 1 | 6 | 102 | 58 | 155 | 2.34e-20 | 80.1 |
| Msa0439950 | MtrunA17_Chr2g0282951 | 34.653 | 101 | 66 | 0 | 2 | 102 | 27 | 127 | 9.79e-20 | 77.8 |
| Msa0439950 | MtrunA17_Chr5g0434201 | 40.777 | 103 | 59 | 1 | 2 | 102 | 21 | 123 | 7.16e-18 | 72.8 |
| Msa0439950 | MtrunA17_Chr3g0117411 | 34.314 | 102 | 67 | 0 | 1 | 102 | 18 | 119 | 4.49e-17 | 70.5 |
| Msa0439950 | MtrunA17_Chr8g0376641 | 39.806 | 103 | 60 | 1 | 2 | 102 | 21 | 123 | 1.35e-16 | 69.3 |
| Msa0439950 | MtrunA17_Chr1g0151231 | 37.736 | 106 | 56 | 3 | 3 | 102 | 48 | 149 | 4.37e-16 | 68.9 |
| Msa0439950 | MtrunA17_Chr3g0135811 | 35.000 | 100 | 59 | 2 | 3 | 101 | 23 | 117 | 2.22e-15 | 66.2 |
| Msa0439950 | MtrunA17_Chr7g0228311 | 35.000 | 100 | 65 | 0 | 1 | 100 | 3 | 102 | 4.10e-15 | 65.1 |
| Msa0439950 | MtrunA17_Chr3g0117391 | 33.000 | 100 | 67 | 0 | 1 | 100 | 21 | 120 | 5.83e-15 | 65.5 |
| Msa0439950 | MtrunA17_Chr1g0182971 | 35.354 | 99 | 63 | 1 | 2 | 99 | 84 | 182 | 6.20e-14 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0439950 | AT3G21460.1 | 75.490 | 102 | 25 | 0 | 1 | 102 | 1 | 102 | 3.28e-57 | 172 |
| Msa0439950 | AT4G15680.1 | 63.725 | 102 | 37 | 0 | 1 | 102 | 1 | 102 | 1.51e-44 | 140 |
| Msa0439950 | AT4G15700.1 | 63.725 | 102 | 37 | 0 | 1 | 102 | 1 | 102 | 3.88e-44 | 139 |
| Msa0439950 | AT4G15660.1 | 62.745 | 102 | 38 | 0 | 1 | 102 | 1 | 102 | 6.21e-44 | 138 |
| Msa0439950 | AT4G15670.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 9.32e-44 | 138 |
| Msa0439950 | AT5G18600.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 2.10e-43 | 137 |
| Msa0439950 | AT4G15690.1 | 61.765 | 102 | 39 | 0 | 1 | 102 | 1 | 102 | 1.96e-42 | 134 |
| Msa0439950 | AT2G47870.1 | 58.252 | 103 | 42 | 1 | 1 | 102 | 1 | 103 | 1.06e-41 | 132 |
| Msa0439950 | AT3G62950.1 | 60.194 | 103 | 40 | 1 | 1 | 102 | 1 | 103 | 1.52e-41 | 132 |
| Msa0439950 | AT2G30540.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 4.76e-35 | 115 |
| Msa0439950 | AT1G03020.1 | 52.941 | 102 | 48 | 0 | 1 | 102 | 1 | 102 | 1.64e-34 | 114 |
| Msa0439950 | AT3G62930.1 | 51.961 | 102 | 49 | 0 | 1 | 102 | 1 | 102 | 2.00e-34 | 114 |
| Msa0439950 | AT1G06830.1 | 51.579 | 95 | 46 | 0 | 1 | 95 | 1 | 95 | 9.36e-34 | 112 |
| Msa0439950 | AT2G47880.1 | 50.526 | 95 | 47 | 0 | 1 | 95 | 1 | 95 | 5.76e-33 | 110 |
| Msa0439950 | AT3G62960.1 | 49.474 | 95 | 48 | 0 | 1 | 95 | 1 | 95 | 8.89e-32 | 107 |
| Msa0439950 | AT5G14070.1 | 50.000 | 110 | 47 | 2 | 1 | 102 | 31 | 140 | 6.88e-30 | 104 |
| Msa0439950 | AT3G02000.1 | 51.887 | 106 | 45 | 1 | 3 | 102 | 31 | 136 | 2.47e-27 | 97.4 |
| Msa0439950 | AT1G28480.1 | 33.333 | 105 | 66 | 2 | 2 | 102 | 33 | 137 | 8.78e-19 | 75.9 |
| Msa0439950 | AT4G33040.1 | 40.187 | 107 | 54 | 3 | 3 | 102 | 41 | 144 | 8.12e-17 | 70.9 |
| Msa0439950 | AT1G03850.2 | 35.484 | 93 | 56 | 1 | 10 | 102 | 62 | 150 | 3.51e-15 | 67.0 |
| Msa0439950 | AT5G11930.1 | 37.000 | 100 | 53 | 3 | 3 | 101 | 57 | 147 | 6.83e-15 | 66.2 |
| Msa0439950 | AT5G63030.1 | 34.000 | 100 | 66 | 0 | 1 | 100 | 19 | 118 | 2.44e-14 | 63.9 |
| Msa0439950 | AT5G40370.1 | 32.000 | 100 | 68 | 0 | 1 | 100 | 3 | 102 | 1.24e-13 | 62.0 |
| Msa0439950 | AT4G28730.1 | 35.714 | 98 | 62 | 1 | 2 | 98 | 71 | 168 | 1.44e-13 | 63.2 |
| Msa0439950 | AT2G20270.1 | 32.990 | 97 | 64 | 1 | 2 | 97 | 76 | 172 | 7.28e-13 | 61.6 |
| Msa0439950 | AT5G20500.1 | 35.052 | 97 | 63 | 0 | 2 | 98 | 33 | 129 | 2.09e-11 | 56.6 |
Find 27 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGGATCGCATATCGAAAT+TGG | 0.300555 | 3_3:+6435080 | None:intergenic |
| TTAGAGAAGCTGGTGCTCTT+TGG | 0.348544 | 3_3:+6435361 | Msa0439950:CDS |
| ATCGAAATTGGCATCGCAAA+AGG | 0.406599 | 3_3:+6435092 | Msa0439950:CDS |
| AATGAACACTGCTGGAACGC+TGG | 0.408792 | 3_3:-6435268 | None:intergenic |
| AGCGTTCCAGCAGTGTTCAT+TGG | 0.414107 | 3_3:+6435270 | Msa0439950:CDS |
| TCATCTAGCTCATAGATTGC+TGG | 0.425719 | 3_3:-6435192 | None:intergenic |
| ATGAACACTGCTGGAACGCT+GGG | 0.430415 | 3_3:-6435267 | None:intergenic |
| TTCATTGGTGGCAAGTTTGT+GGG | 0.440094 | 3_3:+6435285 | Msa0439950:CDS |
| GTTCATTGGTGGCAAGTTTG+TGG | 0.450468 | 3_3:+6435284 | Msa0439950:CDS |
| AAGAAAATGCTTAGAGAAGC+TGG | 0.460268 | 3_3:+6435351 | Msa0439950:CDS |
| ATGAGCTAGATGAAGATAAG+AGG | 0.471120 | 3_3:+6435202 | Msa0439950:CDS |
| TGAGTGAGCCATTGAGATGA+AGG | 0.478669 | 3_3:-6435329 | None:intergenic |
| TTGCCACCAATGAACACTGC+TGG | 0.487093 | 3_3:-6435276 | None:intergenic |
| GAATGGGCTCTTATAAGATT+AGG | 0.493326 | 3_3:+6435237 | Msa0439950:CDS |
| ATCATGACCCTTCATCTCAA+TGG | 0.503503 | 3_3:+6435321 | Msa0439950:CDS |
| GGATGAGCCATGCAATTAAG+AGG | 0.507985 | 3_3:+6435145 | Msa0439950:CDS |
| ATCTTTAGCAAGAGTTCATG+TGG | 0.520709 | 3_3:+6435123 | Msa0439950:CDS |
| TGAGCTAGATGAAGATAAGA+GGG | 0.550399 | 3_3:+6435203 | Msa0439950:CDS |
| AAAATAGCCTCTTAATTGCA+TGG | 0.552086 | 3_3:-6435152 | None:intergenic |
| GTTCCAGCAGTGTTCATTGG+TGG | 0.567163 | 3_3:+6435273 | Msa0439950:CDS |
| AGCTAGATGAAGATAAGAGG+GGG | 0.570466 | 3_3:+6435205 | Msa0439950:CDS |
| GAAATTGGCATCGCAAAAGG+CGG | 0.634320 | 3_3:+6435095 | Msa0439950:CDS |
| TCTTTAGCAAGAGTTCATGT+GGG | 0.649738 | 3_3:+6435124 | Msa0439950:CDS |
| ATGAAGGGTCATGATTATGT+TGG | 0.653275 | 3_3:-6435313 | None:intergenic |
| GAGTGAGCCATTGAGATGAA+GGG | 0.664431 | 3_3:-6435328 | None:intergenic |
| GAGCTAGATGAAGATAAGAG+GGG | 0.665707 | 3_3:+6435204 | Msa0439950:CDS |
| TGAACACTGCTGGAACGCTG+GGG | 0.703546 | 3_3:-6435266 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAGCCTCTTAATTGCA+TGG | - | chr3_3:6435155-6435174 | None:intergenic | 30.0% | |
| ATCTTTAGCAAGAGTTCATG+TGG | + | chr3_3:6435123-6435142 | Msa0439950:CDS | 35.0% | |
| TCTTTAGCAAGAGTTCATGT+GGG | + | chr3_3:6435124-6435143 | Msa0439950:CDS | 35.0% | |
| !! | AAGAGGCTATTTTATGAGCA+AGG | + | chr3_3:6435162-6435181 | Msa0439950:CDS | 35.0% |
| ! | CTATTTTATGAGCAAGGTGT+TGG | + | chr3_3:6435168-6435187 | Msa0439950:CDS | 35.0% |
| ATGAGCTAGATGAAGATAAG+AGG | + | chr3_3:6435202-6435221 | Msa0439950:CDS | 35.0% | |
| TGAGCTAGATGAAGATAAGA+GGG | + | chr3_3:6435203-6435222 | Msa0439950:CDS | 35.0% | |
| ! | GAATGGGCTCTTATAAGATT+AGG | + | chr3_3:6435237-6435256 | Msa0439950:CDS | 35.0% |
| ATGAAGGGTCATGATTATGT+TGG | - | chr3_3:6435316-6435335 | None:intergenic | 35.0% | |
| AAGAAAATGCTTAGAGAAGC+TGG | + | chr3_3:6435351-6435370 | Msa0439950:CDS | 35.0% | |
| ATCGAAATTGGCATCGCAAA+AGG | + | chr3_3:6435092-6435111 | Msa0439950:CDS | 40.0% | |
| ! | TCATCTAGCTCATAGATTGC+TGG | - | chr3_3:6435195-6435214 | None:intergenic | 40.0% |
| GAGCTAGATGAAGATAAGAG+GGG | + | chr3_3:6435204-6435223 | Msa0439950:CDS | 40.0% | |
| AGCTAGATGAAGATAAGAGG+GGG | + | chr3_3:6435205-6435224 | Msa0439950:CDS | 40.0% | |
| ! | TTCATTGGTGGCAAGTTTGT+GGG | + | chr3_3:6435285-6435304 | Msa0439950:CDS | 40.0% |
| ATCATGACCCTTCATCTCAA+TGG | + | chr3_3:6435321-6435340 | Msa0439950:CDS | 40.0% | |
| GAAATTGGCATCGCAAAAGG+CGG | + | chr3_3:6435095-6435114 | Msa0439950:CDS | 45.0% | |
| GGATGAGCCATGCAATTAAG+AGG | + | chr3_3:6435145-6435164 | Msa0439950:CDS | 45.0% | |
| AGATGAAGATAAGAGGGGGA+AGG | + | chr3_3:6435209-6435228 | Msa0439950:CDS | 45.0% | |
| AGATAAGAGGGGGAAGGAAA+TGG | + | chr3_3:6435215-6435234 | Msa0439950:CDS | 45.0% | |
| ! | GTTCATTGGTGGCAAGTTTG+TGG | + | chr3_3:6435284-6435303 | Msa0439950:CDS | 45.0% |
| GAGTGAGCCATTGAGATGAA+GGG | - | chr3_3:6435331-6435350 | None:intergenic | 45.0% | |
| TGAGTGAGCCATTGAGATGA+AGG | - | chr3_3:6435332-6435351 | None:intergenic | 45.0% | |
| TTAGAGAAGCTGGTGCTCTT+TGG | + | chr3_3:6435361-6435380 | Msa0439950:CDS | 45.0% | |
| AGAGGGGGAAGGAAATGGAA+TGG | + | chr3_3:6435220-6435239 | Msa0439950:CDS | 50.0% | |
| GAGGGGGAAGGAAATGGAAT+GGG | + | chr3_3:6435221-6435240 | Msa0439950:CDS | 50.0% | |
| ATGAACACTGCTGGAACGCT+GGG | - | chr3_3:6435270-6435289 | None:intergenic | 50.0% | |
| AATGAACACTGCTGGAACGC+TGG | - | chr3_3:6435271-6435290 | None:intergenic | 50.0% | |
| AGCGTTCCAGCAGTGTTCAT+TGG | + | chr3_3:6435270-6435289 | Msa0439950:CDS | 50.0% | |
| GTTCCAGCAGTGTTCATTGG+TGG | + | chr3_3:6435273-6435292 | Msa0439950:CDS | 50.0% | |
| TTGCCACCAATGAACACTGC+TGG | - | chr3_3:6435279-6435298 | None:intergenic | 50.0% | |
| TGAACACTGCTGGAACGCTG+GGG | - | chr3_3:6435269-6435288 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 6435081 | 6435389 | 6435081 | ID=Msa0439950;Name=Msa0439950 |
| chr3_3 | mRNA | 6435081 | 6435389 | 6435081 | ID=Msa0439950-mRNA-1;Parent=Msa0439950;Name=Msa0439950-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr3_3 | exon | 6435081 | 6435389 | 6435081 | ID=Msa0439950-mRNA-1:exon:1326;Parent=Msa0439950-mRNA-1 |
| chr3_3 | CDS | 6435081 | 6435389 | 6435081 | ID=Msa0439950-mRNA-1:cds;Parent=Msa0439950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
