AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0314770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0314770 MtrunA17_Chr2g0285421 97.561 246 6 0 1 246 1 246 0.0 501
Msa0314770 MtrunA17_Chr8g0376721 65.726 248 82 3 1 246 2 248 2.54e-118 338
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 50 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACAATAGTCAAAATT+TGG 0.156428 2_4:+10787170 Msa0314770:five_prime_UTR
CATCATCAGATGTTAGAAAA+TGG 0.212688 2_4:+10788760 Msa0314770:CDS
AATATACAAACAAGAGGATA+TGG 0.344042 2_4:+10787329 Msa0314770:exon
AATCGAGTTTGCGTGTCTTA+TGG 0.347354 2_4:+10787227 Msa0314770:five_prime_UTR
TAGAAGCATCCATGGAAATA+TGG 0.347628 2_4:+10787500 Msa0314770:CDS
ATGGCAGAAGCAATCTATTA+AGG 0.348198 2_4:+10788121 Msa0314770:CDS
AAAATAGAAGGCATGACTTC+AGG 0.371700 2_4:+10788529 Msa0314770:CDS
ACATGAATGCACACAACTAT+TGG 0.377348 2_4:-10788577 None:intergenic
ATTATAGATGATCTTGTTCA+TGG 0.379410 2_4:+10787420 Msa0314770:CDS
TTTCATGTAATCAGATCATA+TGG 0.381243 2_4:+10788090 Msa0314770:intron
CGTTCTAGAAAGCAACCTTA+TGG 0.384289 2_4:+10787196 Msa0314770:five_prime_UTR
TTCAAAGTAGTGTATGTTAG+TGG 0.388260 2_4:+10788706 Msa0314770:CDS
ACCAAGGGTTTCAGTAGTTA+AGG 0.397101 2_4:-10787457 None:intergenic
ATTCTCCAGCATATGCTTCA+AGG 0.397395 2_4:-10788372 None:intergenic
TCATCTATAATCACAATTGT+TGG 0.398129 2_4:-10787408 None:intergenic
CTCGATTCTTCCCTTCCATA+AGG 0.406918 2_4:-10787211 None:intergenic
ATCATCAGATGTTAGAAAAT+GGG 0.425313 2_4:+10788761 Msa0314770:CDS
TTAATTCTGACCATAGGAAA+TGG 0.426007 2_4:+10788635 Msa0314770:CDS
TCACGCTTTGGGAAAATAGA+AGG 0.429485 2_4:+10788517 Msa0314770:CDS
CGGCGTAAGAAAGGTCATTA+AGG 0.433721 2_4:-10788477 None:intergenic
TCAGCTGCAAAATTCTCAAC+TGG 0.441332 2_4:+10788409 Msa0314770:CDS
AAAATGAATAGACTTGGAAA+TGG 0.442992 2_4:-10787085 None:intergenic
CATAAGGTTGCTTTCTAGAA+CGG 0.461658 2_4:-10787195 None:intergenic
ACTTCAGGTCTTTGCTTGAA+AGG 0.470876 2_4:+10788544 Msa0314770:CDS
GAGCTAACACGGCGTAAGAA+AGG 0.484665 2_4:-10788486 None:intergenic
GTCATCTCTACTATTTGTGC+CGG 0.488913 2_4:-10788455 None:intergenic
AGTTTCCTTGAAGCATATGC+TGG 0.496553 2_4:+10788367 Msa0314770:CDS
TCCTTAACTACTGAAACCCT+TGG 0.503695 2_4:+10787456 Msa0314770:CDS
ATATATAAAATGAATAGACT+TGG 0.525533 2_4:-10787091 None:intergenic
TGTAATCAGATCATATGGTA+TGG 0.530296 2_4:+10788095 Msa0314770:intron
AGGAATTTACCATATTTCCA+TGG 0.534844 2_4:-10787509 None:intergenic
AGATGTTAGAAAATGGGAAA+GGG 0.535212 2_4:+10788767 Msa0314770:CDS
AAGGCATCATCCATTTCCTA+TGG 0.542944 2_4:-10788645 None:intergenic
TCTATATTTAGAAGCATCCA+TGG 0.553025 2_4:+10787492 Msa0314770:CDS
CAGATGTTAGAAAATGGGAA+AGG 0.557087 2_4:+10788766 Msa0314770:CDS
CTAGAAAGCAACCTTATGGA+AGG 0.558094 2_4:+10787200 Msa0314770:five_prime_UTR
ATATTATCTATCAACTCAGC+CGG 0.573023 2_4:+10788436 Msa0314770:CDS
GGATACTTAATTCTGACCAT+AGG 0.582839 2_4:+10788629 Msa0314770:CDS
ATTAAGAATATACAAACAAG+AGG 0.592359 2_4:+10787323 Msa0314770:five_prime_UTR
AGAGCTTTGAACACAACCAA+GGG 0.600437 2_4:-10787472 None:intergenic
AGATCATATGGTATGGAGCA+TGG 0.601542 2_4:+10788102 Msa0314770:intron
TAGAAAGCAACCTTATGGAA+GGG 0.613123 2_4:+10787201 Msa0314770:five_prime_UTR
AGAGGATATGGCATGTTCTG+TGG 0.613331 2_4:+10787341 Msa0314770:exon
CTTGGAAATGGAACGTAAAG+CGG 0.619711 2_4:-10787073 None:intergenic
ACAGAAATCAATGTTAACCA+AGG 0.624182 2_4:+10788315 Msa0314770:intron
TAGAGCTTTGAACACAACCA+AGG 0.633504 2_4:-10787473 None:intergenic
GTTAACCAAGGTATCAACCA+TGG 0.651380 2_4:+10788327 Msa0314770:CDS
TATGCTGGAGAATCAAGAGA+TGG 0.654209 2_4:+10788382 Msa0314770:CDS
TGACCTGAAAAGAGCTAACA+CGG 0.676515 2_4:-10788497 None:intergenic
TAGTTGTGTGCATTCATGTG+TGG 0.691763 2_4:+10788581 Msa0314770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATATATTTTATTATCTG+TGG + chr2_4:10787105-10787124 Msa0314770:five_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG - chr2_4:10788276-10788295 None:intergenic 10.0%
!!! TTTATATATTTTATTATCTG+TGG + chr2_4:10787105-10787124 Msa0314770:five_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG - chr2_4:10788276-10788295 None:intergenic 10.0%
!! ATATATAAAATGAATAGACT+TGG - chr2_4:10787094-10787113 None:intergenic 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG - chr2_4:10787553-10787572 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG + chr2_4:10787737-10787756 Msa0314770:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG - chr2_4:10788272-10788291 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG - chr2_4:10788273-10788292 None:intergenic 15.0%
!! TGAAAGAATGAATAAATATT+AGG + chr2_4:10788932-10788951 Msa0314770:three_prime_UTR 15.0%
!! ATATATAAAATGAATAGACT+TGG - chr2_4:10787094-10787113 None:intergenic 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG - chr2_4:10787553-10787572 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG + chr2_4:10787737-10787756 Msa0314770:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG - chr2_4:10788272-10788291 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG - chr2_4:10788273-10788292 None:intergenic 15.0%
!!! ATATTTTATTATCTGTGGTT+TGG + chr2_4:10787110-10787129 Msa0314770:five_prime_UTR 20.0%
!!! AAATTTTGACTATTGTGTAA+AGG - chr2_4:10787171-10787190 None:intergenic 20.0%
!! TTTACACAATAGTCAAAATT+TGG + chr2_4:10787170-10787189 Msa0314770:five_prime_UTR 20.0%
!! ATTAAGAATATACAAACAAG+AGG + chr2_4:10787323-10787342 Msa0314770:five_prime_UTR 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG + chr2_4:10787893-10787912 Msa0314770:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG - chr2_4:10787951-10787970 None:intergenic 20.0%
!!! ATATTTTATTATCTGTGGTT+TGG + chr2_4:10787110-10787129 Msa0314770:five_prime_UTR 20.0%
!!! AAATTTTGACTATTGTGTAA+AGG - chr2_4:10787171-10787190 None:intergenic 20.0%
!! TTTACACAATAGTCAAAATT+TGG + chr2_4:10787170-10787189 Msa0314770:five_prime_UTR 20.0%
!! ATTAAGAATATACAAACAAG+AGG + chr2_4:10787323-10787342 Msa0314770:five_prime_UTR 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG + chr2_4:10787893-10787912 Msa0314770:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG - chr2_4:10787951-10787970 None:intergenic 20.0%
! AAAATGAATAGACTTGGAAA+TGG - chr2_4:10787088-10787107 None:intergenic 25.0%
! AATATACAAACAAGAGGATA+TGG + chr2_4:10787329-10787348 Msa0314770:exon 25.0%
! TCATCTATAATCACAATTGT+TGG - chr2_4:10787411-10787430 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG + chr2_4:10787420-10787439 Msa0314770:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG - chr2_4:10787669-10787688 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG + chr2_4:10787852-10787871 Msa0314770:intron 25.0%
!! TCATGTTTGGATTCAATTTT+GGG + chr2_4:10787853-10787872 Msa0314770:intron 25.0%
!!! AAATGATTTTGGTGTATGTT+TGG + chr2_4:10787904-10787923 Msa0314770:intron 25.0%
! TTTCATGTAATCAGATCATA+TGG + chr2_4:10788090-10788109 Msa0314770:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG - chr2_4:10788347-10788366 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG + chr2_4:10788761-10788780 Msa0314770:CDS 25.0%
! AAAATGAATAGACTTGGAAA+TGG - chr2_4:10787088-10787107 None:intergenic 25.0%
! AATATACAAACAAGAGGATA+TGG + chr2_4:10787329-10787348 Msa0314770:exon 25.0%
! TCATCTATAATCACAATTGT+TGG - chr2_4:10787411-10787430 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG + chr2_4:10787420-10787439 Msa0314770:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG - chr2_4:10787669-10787688 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG + chr2_4:10787852-10787871 Msa0314770:intron 25.0%
!! TCATGTTTGGATTCAATTTT+GGG + chr2_4:10787853-10787872 Msa0314770:intron 25.0%
!!! AAATGATTTTGGTGTATGTT+TGG + chr2_4:10787904-10787923 Msa0314770:intron 25.0%
! TTTCATGTAATCAGATCATA+TGG + chr2_4:10788090-10788109 Msa0314770:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG - chr2_4:10788347-10788366 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG + chr2_4:10788761-10788780 Msa0314770:CDS 25.0%
!!! CATTTTGAGAGTGTTCAAAT+TGG - chr2_4:10787262-10787281 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG + chr2_4:10787362-10787381 Msa0314770:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG + chr2_4:10787492-10787511 Msa0314770:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG - chr2_4:10787512-10787531 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG - chr2_4:10787629-10787648 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG - chr2_4:10787630-10787649 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG - chr2_4:10787678-10787697 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG + chr2_4:10787684-10787703 Msa0314770:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG + chr2_4:10787776-10787795 Msa0314770:intron 30.0%
TGTAATCAGATCATATGGTA+TGG + chr2_4:10788095-10788114 Msa0314770:intron 30.0%
ATGCATACAAAACATCTGAA+AGG - chr2_4:10788191-10788210 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG - chr2_4:10788257-10788276 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG + chr2_4:10788315-10788334 Msa0314770:intron 30.0%
ATATTATCTATCAACTCAGC+CGG + chr2_4:10788436-10788455 Msa0314770:CDS 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG + chr2_4:10788608-10788627 Msa0314770:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG + chr2_4:10788635-10788654 Msa0314770:CDS 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG - chr2_4:10788667-10788686 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG + chr2_4:10788706-10788725 Msa0314770:CDS 30.0%
CATCATCAGATGTTAGAAAA+TGG + chr2_4:10788760-10788779 Msa0314770:CDS 30.0%
AGATGTTAGAAAATGGGAAA+GGG + chr2_4:10788767-10788786 Msa0314770:CDS 30.0%
AGTGAAAAATAACTTAGCAG+AGG - chr2_4:10788861-10788880 None:intergenic 30.0%
!!! CATTTTGAGAGTGTTCAAAT+TGG - chr2_4:10787262-10787281 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG + chr2_4:10787362-10787381 Msa0314770:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG + chr2_4:10787492-10787511 Msa0314770:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG - chr2_4:10787512-10787531 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG - chr2_4:10787629-10787648 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG - chr2_4:10787630-10787649 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG - chr2_4:10787678-10787697 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG + chr2_4:10787684-10787703 Msa0314770:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG + chr2_4:10787776-10787795 Msa0314770:intron 30.0%
TGTAATCAGATCATATGGTA+TGG + chr2_4:10788095-10788114 Msa0314770:intron 30.0%
ATGCATACAAAACATCTGAA+AGG - chr2_4:10788191-10788210 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG - chr2_4:10788257-10788276 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG + chr2_4:10788315-10788334 Msa0314770:intron 30.0%
ATATTATCTATCAACTCAGC+CGG + chr2_4:10788436-10788455 Msa0314770:CDS 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG + chr2_4:10788608-10788627 Msa0314770:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG + chr2_4:10788635-10788654 Msa0314770:CDS 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG - chr2_4:10788667-10788686 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG + chr2_4:10788706-10788725 Msa0314770:CDS 30.0%
CATCATCAGATGTTAGAAAA+TGG + chr2_4:10788760-10788779 Msa0314770:CDS 30.0%
AGATGTTAGAAAATGGGAAA+GGG + chr2_4:10788767-10788786 Msa0314770:CDS 30.0%
AGTGAAAAATAACTTAGCAG+AGG - chr2_4:10788861-10788880 None:intergenic 30.0%
GAAACAAAAACAAACAGCAG+TGG + chr2_4:10787136-10787155 Msa0314770:five_prime_UTR 35.0%
! CATAAGGTTGCTTTCTAGAA+CGG - chr2_4:10787198-10787217 None:intergenic 35.0%
TAGAAAGCAACCTTATGGAA+GGG + chr2_4:10787201-10787220 Msa0314770:five_prime_UTR 35.0%
!! TAGAAGCATCCATGGAAATA+TGG + chr2_4:10787500-10787519 Msa0314770:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG - chr2_4:10787532-10787551 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG - chr2_4:10787846-10787865 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG - chr2_4:10788043-10788062 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG + chr2_4:10788121-10788140 Msa0314770:CDS 35.0%
! AAAATAGAAGGCATGACTTC+AGG + chr2_4:10788529-10788548 Msa0314770:CDS 35.0%
ACATGAATGCACACAACTAT+TGG - chr2_4:10788580-10788599 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG + chr2_4:10788629-10788648 Msa0314770:CDS 35.0%
CAGATGTTAGAAAATGGGAA+AGG + chr2_4:10788766-10788785 Msa0314770:CDS 35.0%
GAAACAAAAACAAACAGCAG+TGG + chr2_4:10787136-10787155 Msa0314770:five_prime_UTR 35.0%
! CATAAGGTTGCTTTCTAGAA+CGG - chr2_4:10787198-10787217 None:intergenic 35.0%
TAGAAAGCAACCTTATGGAA+GGG + chr2_4:10787201-10787220 Msa0314770:five_prime_UTR 35.0%
!! TAGAAGCATCCATGGAAATA+TGG + chr2_4:10787500-10787519 Msa0314770:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG - chr2_4:10787532-10787551 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG - chr2_4:10787846-10787865 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG - chr2_4:10788043-10788062 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG + chr2_4:10788121-10788140 Msa0314770:CDS 35.0%
! AAAATAGAAGGCATGACTTC+AGG + chr2_4:10788529-10788548 Msa0314770:CDS 35.0%
ACATGAATGCACACAACTAT+TGG - chr2_4:10788580-10788599 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG + chr2_4:10788629-10788648 Msa0314770:CDS 35.0%
CAGATGTTAGAAAATGGGAA+AGG + chr2_4:10788766-10788785 Msa0314770:CDS 35.0%
CTTGGAAATGGAACGTAAAG+CGG - chr2_4:10787076-10787095 None:intergenic 40.0%
!! CGTTCTAGAAAGCAACCTTA+TGG + chr2_4:10787196-10787215 Msa0314770:five_prime_UTR 40.0%
CTAGAAAGCAACCTTATGGA+AGG + chr2_4:10787200-10787219 Msa0314770:five_prime_UTR 40.0%
AATCGAGTTTGCGTGTCTTA+TGG + chr2_4:10787227-10787246 Msa0314770:five_prime_UTR 40.0%
TCCTTAACTACTGAAACCCT+TGG + chr2_4:10787456-10787475 Msa0314770:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG - chr2_4:10787460-10787479 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG - chr2_4:10787475-10787494 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG - chr2_4:10787476-10787495 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG + chr2_4:10787691-10787710 Msa0314770:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG - chr2_4:10787812-10787831 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG - chr2_4:10787982-10788001 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG + chr2_4:10788102-10788121 Msa0314770:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG + chr2_4:10788327-10788346 Msa0314770:CDS 40.0%
!! TTTTGCCATGGTTGATACCT+TGG - chr2_4:10788335-10788354 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG + chr2_4:10788367-10788386 Msa0314770:CDS 40.0%
ATTCTCCAGCATATGCTTCA+AGG - chr2_4:10788375-10788394 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr2_4:10788382-10788401 Msa0314770:CDS 40.0%
TCAGCTGCAAAATTCTCAAC+TGG + chr2_4:10788409-10788428 Msa0314770:CDS 40.0%
GTCATCTCTACTATTTGTGC+CGG - chr2_4:10788458-10788477 None:intergenic 40.0%
TGACCTGAAAAGAGCTAACA+CGG - chr2_4:10788500-10788519 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG + chr2_4:10788517-10788536 Msa0314770:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG + chr2_4:10788544-10788563 Msa0314770:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG + chr2_4:10788581-10788600 Msa0314770:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG - chr2_4:10788648-10788667 None:intergenic 40.0%
CTTGGAAATGGAACGTAAAG+CGG - chr2_4:10787076-10787095 None:intergenic 40.0%
!! CGTTCTAGAAAGCAACCTTA+TGG + chr2_4:10787196-10787215 Msa0314770:five_prime_UTR 40.0%
CTAGAAAGCAACCTTATGGA+AGG + chr2_4:10787200-10787219 Msa0314770:five_prime_UTR 40.0%
AATCGAGTTTGCGTGTCTTA+TGG + chr2_4:10787227-10787246 Msa0314770:five_prime_UTR 40.0%
TCCTTAACTACTGAAACCCT+TGG + chr2_4:10787456-10787475 Msa0314770:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG - chr2_4:10787460-10787479 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG - chr2_4:10787475-10787494 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG - chr2_4:10787476-10787495 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG + chr2_4:10787691-10787710 Msa0314770:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG - chr2_4:10787812-10787831 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG - chr2_4:10787982-10788001 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG + chr2_4:10788102-10788121 Msa0314770:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG + chr2_4:10788327-10788346 Msa0314770:CDS 40.0%
!! TTTTGCCATGGTTGATACCT+TGG - chr2_4:10788335-10788354 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG + chr2_4:10788367-10788386 Msa0314770:CDS 40.0%
ATTCTCCAGCATATGCTTCA+AGG - chr2_4:10788375-10788394 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr2_4:10788382-10788401 Msa0314770:CDS 40.0%
TCAGCTGCAAAATTCTCAAC+TGG + chr2_4:10788409-10788428 Msa0314770:CDS 40.0%
GTCATCTCTACTATTTGTGC+CGG - chr2_4:10788458-10788477 None:intergenic 40.0%
TGACCTGAAAAGAGCTAACA+CGG - chr2_4:10788500-10788519 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG + chr2_4:10788517-10788536 Msa0314770:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG + chr2_4:10788544-10788563 Msa0314770:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG + chr2_4:10788581-10788600 Msa0314770:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG - chr2_4:10788648-10788667 None:intergenic 40.0%
CTCGATTCTTCCCTTCCATA+AGG - chr2_4:10787214-10787233 None:intergenic 45.0%
AGAGGATATGGCATGTTCTG+TGG + chr2_4:10787341-10787360 Msa0314770:exon 45.0%
CATGAACTACCATGGAGCAA+TGG - chr2_4:10787838-10787857 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG + chr2_4:10787840-10787859 Msa0314770:intron 45.0%
CGGCGTAAGAAAGGTCATTA+AGG - chr2_4:10788480-10788499 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG + chr2_4:10788506-10788525 Msa0314770:CDS 45.0%
CTCGATTCTTCCCTTCCATA+AGG - chr2_4:10787214-10787233 None:intergenic 45.0%
AGAGGATATGGCATGTTCTG+TGG + chr2_4:10787341-10787360 Msa0314770:exon 45.0%
CATGAACTACCATGGAGCAA+TGG - chr2_4:10787838-10787857 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG + chr2_4:10787840-10787859 Msa0314770:intron 45.0%
CGGCGTAAGAAAGGTCATTA+AGG - chr2_4:10788480-10788499 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG + chr2_4:10788506-10788525 Msa0314770:CDS 45.0%
CTTCAGATGCCATTGCTCCA+TGG + chr2_4:10787826-10787845 Msa0314770:intron 50.0%
GAGCTAACACGGCGTAAGAA+AGG - chr2_4:10788489-10788508 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG + chr2_4:10788494-10788513 Msa0314770:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG + chr2_4:10788505-10788524 Msa0314770:CDS 50.0%
CTTCAGATGCCATTGCTCCA+TGG + chr2_4:10787826-10787845 Msa0314770:intron 50.0%
GAGCTAACACGGCGTAAGAA+AGG - chr2_4:10788489-10788508 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG + chr2_4:10788494-10788513 Msa0314770:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG + chr2_4:10788505-10788524 Msa0314770:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 10787049 10788953 10787049 ID=Msa0314770;Name=Msa0314770
chr2_4 mRNA 10787049 10788953 10787049 ID=Msa0314770-mRNA-1;Parent=Msa0314770;Name=Msa0314770-mRNA-1;_AED=0.08;_eAED=0.08;_QI=299|1|1|1|1|1|3|134|246
chr2_4 exon 10787049 10787521 10787049 ID=Msa0314770-mRNA-1:exon:4077;Parent=Msa0314770-mRNA-1
chr2_4 exon 10788104 10788169 10788104 ID=Msa0314770-mRNA-1:exon:4078;Parent=Msa0314770-mRNA-1
chr2_4 exon 10788319 10788953 10788319 ID=Msa0314770-mRNA-1:exon:4079;Parent=Msa0314770-mRNA-1
chr2_4 five_prime_UTR 10787049 10787347 10787049 ID=Msa0314770-mRNA-1:five_prime_utr;Parent=Msa0314770-mRNA-1
chr2_4 CDS 10787348 10787521 10787348 ID=Msa0314770-mRNA-1:cds;Parent=Msa0314770-mRNA-1
chr2_4 CDS 10788104 10788169 10788104 ID=Msa0314770-mRNA-1:cds;Parent=Msa0314770-mRNA-1
chr2_4 CDS 10788319 10788819 10788319 ID=Msa0314770-mRNA-1:cds;Parent=Msa0314770-mRNA-1
chr2_4 three_prime_UTR 10788820 10788953 10788820 ID=Msa0314770-mRNA-1:three_prime_utr;Parent=Msa0314770-mRNA-1
Gene Sequence

>Msa0314770

ATGGCATGTTCTGTGGCTTTTAACAACAAGAATTTGGAAATAAGTTTCTTTGTTTTCAAGCCAACAATTGTGATTATAGATGATCTTGTTCATGGTCTCAAACAATTCTCCTTAACTACTGAAACCCTTGGTTGTGTTCAAAGCTCTATATTTAGAAGCATCCATGGAAATATGATCATATGGTATGGAGCATGGCAGAAGCAATCTATTAAGGAAAAAGAGCTTCTAACTTCAACTCTTAAATCAATGTTAACCAAGGTATCAACCATGGCAAAATTAATTGAACATAGTTTCCTTGAAGCATATGCTGGAGAATCAAGAGATGGCTCATCAGCTGCAAAATTCTCAACTGGAGACATATTATCTATCAACTCAGCCGGCACAAATAGTAGAGATGACCTTAATGACCTTTCTTACGCCGTGTTAGCTCTTTTCAGGTCACGCTTTGGGAAAATAGAAGGCATGACTTCAGGTCTTTGCTTGAAAGGACAAAGCAGACCAATAGTTGTGTGCATTCATGTGTGGAAATCTCTTCATTTTTGTTACTCATGGATACTTAATTCTGACCATAGGAAATGGATGATGCCTTATCTTGAAAAATTCGCGATCGATATGAAGTATGATATTTTCAAAGTAGTGTATGTTAGTGGTGACAATGTTGTTGATGTTAACTATATTTCTCATCATCAGATGTTAGAAAATGGGAAAGGGAGCATAGAAAGACAGATAATGCAGAACTGA
Protein sequence

>Msa0314770

MACSVAFNNKNLEISFFVFKPTIVIIDDLVHGLKQFSLTTETLGCVQSSIFRSIHGNMIIWYGAWQKQSIKEKELLTSTLKSMLTKVSTMAKLIEHSFLEAYAGESRDGSSAAKFSTGDILSINSAGTNSRDDLNDLSYAVLALFRSRFGKIEGMTSGLCLKGQSRPIVVCIHVWKSLHFCYSWILNSDHRKWMMPYLEKFAIDMKYDIFKVVYVSGDNVVDVNYISHHQMLENGKGSIERQIMQN*