AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1448700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1448700 MtrunA17_Chr2g0285421 97.561 246 6 0 1 246 1 246 0.0 501
Msa1448700 MtrunA17_Chr8g0376721 65.726 248 82 3 1 246 2 248 2.54e-118 338
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACCATAGTCAAAATT+TGG 0.163249 tig0032298:-20060 Msa1448700:five_prime_UTR
CATCATCAGATGTTAGAAAA+TGG 0.212688 tig0032298:-18437 Msa1448700:CDS
TCATTTGAGAGTGTTCAAAT+TGG 0.324071 tig0032298:+19976 None:intergenic
TAGAAGCATCCATGGAAATA+TGG 0.347628 tig0032298:-19707 Msa1448700:intron
ATGGCAGAAGCAATCTATTA+AGG 0.348198 tig0032298:-19076 Msa1448700:CDS
AGTCGAGTTTGCGTGTCTTA+TGG 0.356753 tig0032298:-20008 Msa1448700:five_prime_UTR
AAAATAGAAGGCATGACTTC+AGG 0.371700 tig0032298:-18668 Msa1448700:CDS
AATATACAAACAAAAGGATA+TGG 0.372931 tig0032298:-19878 Msa1448700:exon
ACATGAATGCACACAACTAT+TGG 0.377348 tig0032298:+18620 None:intergenic
ATTATAGATGATCTTGTTCA+TGG 0.379410 tig0032298:-19787 Msa1448700:CDS
TATAGTAGAAGTAAAGTAGT+AGG 0.380385 tig0032298:-19927 Msa1448700:five_prime_UTR
TTTCATGTAATCAGATCATA+TGG 0.381243 tig0032298:-19107 Msa1448700:intron
TTCAAAGTAGTGTATGTTAG+TGG 0.388260 tig0032298:-18491 Msa1448700:CDS
ACCAAGGGTTTCAGTAGTTA+AGG 0.397101 tig0032298:+19750 None:intergenic
ATTCTCCAGCATATGCTTCA+AGG 0.397395 tig0032298:+18825 None:intergenic
TCATCTATAATCACAATTGT+TGG 0.398129 tig0032298:+19799 None:intergenic
GCAAACTCGACTCTTCTATA+AGG 0.418674 tig0032298:+20019 None:intergenic
ATCATCAGATGTTAGAAAAT+GGG 0.425313 tig0032298:-18436 Msa1448700:CDS
TTAATTCTGACCATAGGAAA+TGG 0.426007 tig0032298:-18562 Msa1448700:CDS
TCACGCTTTGGGAAAATAGA+AGG 0.429485 tig0032298:-18680 Msa1448700:CDS
CGGCGTAAGAAAGGTCATTA+AGG 0.433721 tig0032298:+18720 None:intergenic
TCAGCTGCAAAATTCTCAAC+TGG 0.441332 tig0032298:-18788 Msa1448700:CDS
AAAATGAATAGACTTGGAAA+TGG 0.442992 tig0032298:+20147 None:intergenic
ACTTCAGGTCTTTGCTTGAA+AGG 0.470876 tig0032298:-18653 Msa1448700:CDS
TATAAGGTTGCTTTCTAGAA+CGG 0.481854 tig0032298:+20035 None:intergenic
GAGCTAACACGGCGTAAGAA+AGG 0.484665 tig0032298:+18711 None:intergenic
AGTTTCCTTGAAGCATATGC+TGG 0.496553 tig0032298:-18830 Msa1448700:CDS
ATTAAGAATATACAAACAAA+AGG 0.502484 tig0032298:-19884 Msa1448700:five_prime_UTR
TCCTTAACTACTGAAACCCT+TGG 0.503695 tig0032298:-19751 Msa1448700:CDS
GTCATCTCTACTATTCGTGC+CGG 0.513748 tig0032298:+18742 None:intergenic
ATATATAAAATGAATAGACT+TGG 0.525533 tig0032298:+20141 None:intergenic
TGTAATCAGATCATATGGTA+TGG 0.530296 tig0032298:-19102 Msa1448700:intron
AGGAATTTACCATATTTCCA+TGG 0.534844 tig0032298:+19698 None:intergenic
AGATGTTAGAAAATGGGAAA+GGG 0.535212 tig0032298:-18430 Msa1448700:CDS
AAGGCATCATCCATTTCCTA+TGG 0.542944 tig0032298:+18552 None:intergenic
TCTATATTTAGAAGCATCCA+TGG 0.553025 tig0032298:-19715 Msa1448700:CDS
CAGATGTTAGAAAATGGGAA+AGG 0.557087 tig0032298:-18431 Msa1448700:CDS
ATATTATCTATCAACTCAGC+CGG 0.573023 tig0032298:-18761 Msa1448700:CDS
GGATACTTAATTCTGACCAT+AGG 0.582839 tig0032298:-18568 Msa1448700:CDS
AGAGCTTTGAACACAACCAA+GGG 0.600437 tig0032298:+19735 None:intergenic
AGATCATATGGTATGGAGCA+TGG 0.601542 tig0032298:-19095 Msa1448700:CDS
AAAGGATATGGCATGTTCTG+TGG 0.607383 tig0032298:-19866 Msa1448700:exon
CTTGGAAATGGAACGTAAAG+CGG 0.619711 tig0032298:+20159 None:intergenic
ACAGAAATCAATGTTAACCA+AGG 0.624182 tig0032298:-18882 Msa1448700:intron
TAGAGCTTTGAACACAACCA+AGG 0.633504 tig0032298:+19734 None:intergenic
GTTAACCAAGGTATCAACCA+TGG 0.651380 tig0032298:-18870 Msa1448700:CDS
TATGCTGGAGAATCAAGAGA+TGG 0.654209 tig0032298:-18815 Msa1448700:CDS
TGACCTGAAAAGAGCTAACA+CGG 0.676515 tig0032298:+18700 None:intergenic
TAGTTGTGTGCATTCATGTG+TGG 0.691763 tig0032298:-18616 Msa1448700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTATTATCTG+TGG - tig0032298:18324-18343 Msa1448700:three_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG + tig0032298:19528-19547 None:intergenic 10.0%
!!! TATATATTTTTTATTATCTG+TGG - tig0032298:18324-18343 Msa1448700:three_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG + tig0032298:19528-19547 None:intergenic 10.0%
!! ATATATAAAATGAATAGACT+TGG + tig0032298:18311-18330 None:intergenic 15.0%
!! ATTAAGAATATACAAACAAA+AGG - tig0032298:18565-18584 Msa1448700:CDS 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG + tig0032298:18795-18814 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG - tig0032298:18979-18998 Msa1448700:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG + tig0032298:19524-19543 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG + tig0032298:19525-19544 None:intergenic 15.0%
!! TGAAAGAATGAATAAATATT+AGG - tig0032298:20184-20203 Msa1448700:five_prime_UTR 15.0%
!! ATATATAAAATGAATAGACT+TGG + tig0032298:18311-18330 None:intergenic 15.0%
!! ATTAAGAATATACAAACAAA+AGG - tig0032298:18565-18584 Msa1448700:CDS 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG + tig0032298:18795-18814 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG - tig0032298:18979-18998 Msa1448700:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG + tig0032298:19524-19543 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG + tig0032298:19525-19544 None:intergenic 15.0%
!! AATATACAAACAAAAGGATA+TGG - tig0032298:18571-18590 Msa1448700:CDS 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG - tig0032298:19135-19154 Msa1448700:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG - tig0032298:19144-19163 Msa1448700:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG + tig0032298:19191-19210 None:intergenic 20.0%
!! AATATACAAACAAAAGGATA+TGG - tig0032298:18571-18590 Msa1448700:CDS 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG - tig0032298:19135-19154 Msa1448700:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG - tig0032298:19144-19163 Msa1448700:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG + tig0032298:19191-19210 None:intergenic 20.0%
! AAAATGAATAGACTTGGAAA+TGG + tig0032298:18305-18324 None:intergenic 25.0%
!!! AAATTTTGACTATGGTGTAA+AGG + tig0032298:18390-18409 None:intergenic 25.0%
! TTTACACCATAGTCAAAATT+TGG - tig0032298:18389-18408 Msa1448700:exon 25.0%
! TATAGTAGAAGTAAAGTAGT+AGG - tig0032298:18522-18541 Msa1448700:CDS 25.0%
! TCATCTATAATCACAATTGT+TGG + tig0032298:18653-18672 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG - tig0032298:18662-18681 Msa1448700:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG + tig0032298:18911-18930 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG - tig0032298:19094-19113 Msa1448700:CDS 25.0%
!! TCATGTTTGGATTCAATTTT+GGG - tig0032298:19095-19114 Msa1448700:CDS 25.0%
! TTTCATGTAATCAGATCATA+TGG - tig0032298:19342-19361 Msa1448700:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG + tig0032298:19599-19618 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG - tig0032298:20013-20032 Msa1448700:five_prime_UTR 25.0%
! AAAATGAATAGACTTGGAAA+TGG + tig0032298:18305-18324 None:intergenic 25.0%
!!! AAATTTTGACTATGGTGTAA+AGG + tig0032298:18390-18409 None:intergenic 25.0%
! TTTACACCATAGTCAAAATT+TGG - tig0032298:18389-18408 Msa1448700:exon 25.0%
! TATAGTAGAAGTAAAGTAGT+AGG - tig0032298:18522-18541 Msa1448700:CDS 25.0%
! TCATCTATAATCACAATTGT+TGG + tig0032298:18653-18672 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG - tig0032298:18662-18681 Msa1448700:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG + tig0032298:18911-18930 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG - tig0032298:19094-19113 Msa1448700:CDS 25.0%
!! TCATGTTTGGATTCAATTTT+GGG - tig0032298:19095-19114 Msa1448700:CDS 25.0%
! TTTCATGTAATCAGATCATA+TGG - tig0032298:19342-19361 Msa1448700:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG + tig0032298:19599-19618 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG - tig0032298:20013-20032 Msa1448700:five_prime_UTR 25.0%
GAAACAAAAACAAACAACAG+TGG - tig0032298:18355-18374 Msa1448700:three_prime_UTR 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG + tig0032298:18417-18436 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG + tig0032298:18476-18495 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG - tig0032298:18604-18623 Msa1448700:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG - tig0032298:18734-18753 Msa1448700:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG + tig0032298:18754-18773 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG + tig0032298:18871-18890 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG + tig0032298:18872-18891 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG + tig0032298:18920-18939 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG - tig0032298:18926-18945 Msa1448700:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG - tig0032298:19018-19037 Msa1448700:intron 30.0%
TGTAATCAGATCATATGGTA+TGG - tig0032298:19347-19366 Msa1448700:intron 30.0%
ATGCATACAAAACATCTGAA+AGG + tig0032298:19443-19462 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG + tig0032298:19509-19528 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG - tig0032298:19567-19586 Msa1448700:intron 30.0%
ATATTATCTATCAACTCAGC+CGG - tig0032298:19688-19707 Msa1448700:intron 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG - tig0032298:19860-19879 Msa1448700:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG - tig0032298:19887-19906 Msa1448700:five_prime_UTR 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG + tig0032298:19919-19938 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG - tig0032298:19958-19977 Msa1448700:five_prime_UTR 30.0%
CATCATCAGATGTTAGAAAA+TGG - tig0032298:20012-20031 Msa1448700:five_prime_UTR 30.0%
AGATGTTAGAAAATGGGAAA+GGG - tig0032298:20019-20038 Msa1448700:five_prime_UTR 30.0%
AGTGAAAAATAACTTAGCAG+AGG + tig0032298:20113-20132 None:intergenic 30.0%
GAAACAAAAACAAACAACAG+TGG - tig0032298:18355-18374 Msa1448700:three_prime_UTR 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG + tig0032298:18417-18436 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG + tig0032298:18476-18495 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG - tig0032298:18604-18623 Msa1448700:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG - tig0032298:18734-18753 Msa1448700:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG + tig0032298:18754-18773 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG + tig0032298:18871-18890 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG + tig0032298:18872-18891 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG + tig0032298:18920-18939 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG - tig0032298:18926-18945 Msa1448700:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG - tig0032298:19018-19037 Msa1448700:intron 30.0%
TGTAATCAGATCATATGGTA+TGG - tig0032298:19347-19366 Msa1448700:intron 30.0%
ATGCATACAAAACATCTGAA+AGG + tig0032298:19443-19462 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG + tig0032298:19509-19528 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG - tig0032298:19567-19586 Msa1448700:intron 30.0%
ATATTATCTATCAACTCAGC+CGG - tig0032298:19688-19707 Msa1448700:intron 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG - tig0032298:19860-19879 Msa1448700:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG - tig0032298:19887-19906 Msa1448700:five_prime_UTR 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG + tig0032298:19919-19938 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG - tig0032298:19958-19977 Msa1448700:five_prime_UTR 30.0%
CATCATCAGATGTTAGAAAA+TGG - tig0032298:20012-20031 Msa1448700:five_prime_UTR 30.0%
AGATGTTAGAAAATGGGAAA+GGG - tig0032298:20019-20038 Msa1448700:five_prime_UTR 30.0%
AGTGAAAAATAACTTAGCAG+AGG + tig0032298:20113-20132 None:intergenic 30.0%
!!! ACGGTTCCAAATTTTGACTA+TGG + tig0032298:18398-18417 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG - tig0032298:18742-18761 Msa1448700:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG + tig0032298:18774-18793 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG + tig0032298:19088-19107 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG + tig0032298:19295-19314 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG - tig0032298:19373-19392 Msa1448700:intron 35.0%
! AAAATAGAAGGCATGACTTC+AGG - tig0032298:19781-19800 Msa1448700:CDS 35.0%
ACATGAATGCACACAACTAT+TGG + tig0032298:19832-19851 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG - tig0032298:19881-19900 Msa1448700:exon 35.0%
CAGATGTTAGAAAATGGGAA+AGG - tig0032298:20018-20037 Msa1448700:five_prime_UTR 35.0%
!!! ACGGTTCCAAATTTTGACTA+TGG + tig0032298:18398-18417 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG - tig0032298:18742-18761 Msa1448700:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG + tig0032298:18774-18793 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG + tig0032298:19088-19107 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG + tig0032298:19295-19314 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG - tig0032298:19373-19392 Msa1448700:intron 35.0%
! AAAATAGAAGGCATGACTTC+AGG - tig0032298:19781-19800 Msa1448700:CDS 35.0%
ACATGAATGCACACAACTAT+TGG + tig0032298:19832-19851 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG - tig0032298:19881-19900 Msa1448700:exon 35.0%
CAGATGTTAGAAAATGGGAA+AGG - tig0032298:20018-20037 Msa1448700:five_prime_UTR 35.0%
CTTGGAAATGGAACGTAAAG+CGG + tig0032298:18293-18312 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG + tig0032298:18433-18452 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG - tig0032298:18583-18602 Msa1448700:CDS 40.0%
TCCTTAACTACTGAAACCCT+TGG - tig0032298:18698-18717 Msa1448700:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG + tig0032298:18702-18721 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG + tig0032298:18717-18736 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG + tig0032298:18718-18737 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG - tig0032298:18933-18952 Msa1448700:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG + tig0032298:19054-19073 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG + tig0032298:19222-19241 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG - tig0032298:19354-19373 Msa1448700:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG - tig0032298:19579-19598 Msa1448700:intron 40.0%
!! TTTTGCCATGGTTGATACCT+TGG + tig0032298:19587-19606 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG - tig0032298:19619-19638 Msa1448700:intron 40.0%
ATTCTCCAGCATATGCTTCA+AGG + tig0032298:19627-19646 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG - tig0032298:19634-19653 Msa1448700:intron 40.0%
TCAGCTGCAAAATTCTCAAC+TGG - tig0032298:19661-19680 Msa1448700:intron 40.0%
TGACCTGAAAAGAGCTAACA+CGG + tig0032298:19752-19771 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG - tig0032298:19769-19788 Msa1448700:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG - tig0032298:19796-19815 Msa1448700:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG - tig0032298:19833-19852 Msa1448700:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG + tig0032298:19900-19919 None:intergenic 40.0%
CTTGGAAATGGAACGTAAAG+CGG + tig0032298:18293-18312 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG + tig0032298:18433-18452 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG - tig0032298:18583-18602 Msa1448700:CDS 40.0%
TCCTTAACTACTGAAACCCT+TGG - tig0032298:18698-18717 Msa1448700:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG + tig0032298:18702-18721 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG + tig0032298:18717-18736 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG + tig0032298:18718-18737 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG - tig0032298:18933-18952 Msa1448700:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG + tig0032298:19054-19073 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG + tig0032298:19222-19241 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG - tig0032298:19354-19373 Msa1448700:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG - tig0032298:19579-19598 Msa1448700:intron 40.0%
!! TTTTGCCATGGTTGATACCT+TGG + tig0032298:19587-19606 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG - tig0032298:19619-19638 Msa1448700:intron 40.0%
ATTCTCCAGCATATGCTTCA+AGG + tig0032298:19627-19646 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG - tig0032298:19634-19653 Msa1448700:intron 40.0%
TCAGCTGCAAAATTCTCAAC+TGG - tig0032298:19661-19680 Msa1448700:intron 40.0%
TGACCTGAAAAGAGCTAACA+CGG + tig0032298:19752-19771 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG - tig0032298:19769-19788 Msa1448700:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG - tig0032298:19796-19815 Msa1448700:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG - tig0032298:19833-19852 Msa1448700:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG + tig0032298:19900-19919 None:intergenic 40.0%
AGTCGAGTTTGCGTGTCTTA+TGG - tig0032298:18441-18460 Msa1448700:CDS 45.0%
CATGAACTACCATGGAGCAA+TGG + tig0032298:19080-19099 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG - tig0032298:19082-19101 Msa1448700:CDS 45.0%
GTCATCTCTACTATTCGTGC+CGG + tig0032298:19710-19729 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG + tig0032298:19732-19751 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG - tig0032298:19758-19777 Msa1448700:CDS 45.0%
AGTCGAGTTTGCGTGTCTTA+TGG - tig0032298:18441-18460 Msa1448700:CDS 45.0%
CATGAACTACCATGGAGCAA+TGG + tig0032298:19080-19099 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG - tig0032298:19082-19101 Msa1448700:CDS 45.0%
GTCATCTCTACTATTCGTGC+CGG + tig0032298:19710-19729 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG + tig0032298:19732-19751 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG - tig0032298:19758-19777 Msa1448700:CDS 45.0%
CTTCAGATGCCATTGCTCCA+TGG - tig0032298:19068-19087 Msa1448700:CDS 50.0%
GAGCTAACACGGCGTAAGAA+AGG + tig0032298:19741-19760 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG - tig0032298:19746-19765 Msa1448700:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG - tig0032298:19757-19776 Msa1448700:CDS 50.0%
CTTCAGATGCCATTGCTCCA+TGG - tig0032298:19068-19087 Msa1448700:CDS 50.0%
GAGCTAACACGGCGTAAGAA+AGG + tig0032298:19741-19760 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG - tig0032298:19746-19765 Msa1448700:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG - tig0032298:19757-19776 Msa1448700:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0032298 gene 18266 20205 18266 ID=Msa1448700;Name=Msa1448700
tig0032298 mRNA 18266 20205 18266 ID=Msa1448700-mRNA-1;Parent=Msa1448700;Name=Msa1448700-mRNA-1;_AED=0.08;_eAED=0.08;_QI=324|1|1|1|1|1|3|134|246
tig0032298 exon 18266 18900 18266 ID=Msa1448700-mRNA-1:exon:16570;Parent=Msa1448700-mRNA-1
tig0032298 exon 19050 19115 19050 ID=Msa1448700-mRNA-1:exon:16569;Parent=Msa1448700-mRNA-1
tig0032298 exon 19708 20205 19708 ID=Msa1448700-mRNA-1:exon:16568;Parent=Msa1448700-mRNA-1
tig0032298 five_prime_UTR 19882 20205 19882 ID=Msa1448700-mRNA-1:five_prime_utr;Parent=Msa1448700-mRNA-1
tig0032298 CDS 19708 19881 19708 ID=Msa1448700-mRNA-1:cds;Parent=Msa1448700-mRNA-1
tig0032298 CDS 19050 19115 19050 ID=Msa1448700-mRNA-1:cds;Parent=Msa1448700-mRNA-1
tig0032298 CDS 18400 18900 18400 ID=Msa1448700-mRNA-1:cds;Parent=Msa1448700-mRNA-1
tig0032298 three_prime_UTR 18266 18399 18266 ID=Msa1448700-mRNA-1:three_prime_utr;Parent=Msa1448700-mRNA-1
Gene Sequence

>Msa1448700

ATGGCATGTTCTGTGGCTTTTAACAACAAGAATTTGGAAATAAGTTTCTTTGTTTTCAAGCCAACAATTGTGATTATAGATGATCTTGTTCATGGTCTCAAACAATTCTCCTTAACTACTGAAACCCTTGGTTGTGTTCAAAGCTCTATATTTAGAAGCATCCATGGAAATATGATCATATGGTATGGAGCATGGCAGAAGCAATCTATTAAGGAAAAAGAGCTTCTAACTTCAACTCTTAAATCAATGTTAACCAAGGTATCAACCATGGCAAAATTAATTGAACATAGTTTCCTTGAAGCATATGCTGGAGAATCAAGAGATGGCTCATCAGCTGCAAAATTCTCAACTGGAGACATATTATCTATCAACTCAGCCGGCACGAATAGTAGAGATGACCTTAATGACCTTTCTTACGCCGTGTTAGCTCTTTTCAGGTCACGCTTTGGGAAAATAGAAGGCATGACTTCAGGTCTTTGCTTGAAAGGACAAAGCAGACCAATAGTTGTGTGCATTCATGTGTGGAAATCTCTTCATTTTTGTTACTCATGGATACTTAATTCTGACCATAGGAAATGGATGATGCCTTATCTTGAAAAATTCGCGATCGATATGAAGTATGATATTTTCAAAGTAGTGTATGTTAGTGGTGACAATGTTGTTGATGTTAACTATATTTCTCATCATCAGATGTTAGAAAATGGGAAAGGGAGCATAGAAAGACAGATAATGCAGAACTGA
Protein sequence

>Msa1448700

MACSVAFNNKNLEISFFVFKPTIVIIDDLVHGLKQFSLTTETLGCVQSSIFRSIHGNMIIWYGAWQKQSIKEKELLTSTLKSMLTKVSTMAKLIEHSFLEAYAGESRDGSSAAKFSTGDILSINSAGTNSRDDLNDLSYAVLALFRSRFGKIEGMTSGLCLKGQSRPIVVCIHVWKSLHFCYSWILNSDHRKWMMPYLEKFAIDMKYDIFKVVYVSGDNVVDVNYISHHQMLENGKGSIERQIMQN*