AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0187920

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0187920 MtrunA17_Chr2g0285421 97.561 246 6 0 1 246 1 246 0.0 501
Msa0187920 MtrunA17_Chr8g0376721 65.726 248 82 3 1 246 2 248 2.54e-118 338
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACACCATAGTCAAAATT+TGG 0.163249 2_1:+2736289 Msa0187920:five_prime_UTR
CATCATCAGATGTTAGAAAA+TGG 0.212688 2_1:+2737913 Msa0187920:CDS
TCATTTGAGAGTGTTCAAAT+TGG 0.324071 2_1:-2736373 None:intergenic
TAGAAGCATCCATGGAAATA+TGG 0.347628 2_1:+2736645 Msa0187920:CDS
ATGGCAGAAGCAATCTATTA+AGG 0.348198 2_1:+2737274 Msa0187920:CDS
AGTCGAGTTTGCGTGTCTTA+TGG 0.356753 2_1:+2736341 Msa0187920:five_prime_UTR
AAAATAGAAGGCATGACTTC+AGG 0.371700 2_1:+2737682 Msa0187920:CDS
AATATACAAACAAAAGGATA+TGG 0.372931 2_1:+2736474 Msa0187920:exon
ACATGAATGCACACAACTAT+TGG 0.377348 2_1:-2737730 None:intergenic
ATTATAGATGATCTTGTTCA+TGG 0.379410 2_1:+2736565 Msa0187920:CDS
TTTCATGTAATCAGATCATA+TGG 0.381243 2_1:+2737243 Msa0187920:intron
TTCAAAGTAGTGTATGTTAG+TGG 0.388260 2_1:+2737859 Msa0187920:CDS
ACCAAGGGTTTCAGTAGTTA+AGG 0.397101 2_1:-2736602 None:intergenic
ATTCTCCAGCATATGCTTCA+AGG 0.397395 2_1:-2737525 None:intergenic
TCATCTATAATCACAATTGT+TGG 0.398129 2_1:-2736553 None:intergenic
GCAAACTCGACTCTTCTATA+AGG 0.418674 2_1:-2736330 None:intergenic
ATCATCAGATGTTAGAAAAT+GGG 0.425313 2_1:+2737914 Msa0187920:CDS
TTAATTCTGACCATAGGAAA+TGG 0.426007 2_1:+2737788 Msa0187920:CDS
TCACGCTTTGGGAAAATAGA+AGG 0.429485 2_1:+2737670 Msa0187920:CDS
CGGCGTAAGAAAGGTCATTA+AGG 0.433721 2_1:-2737630 None:intergenic
TCAGCTGCAAAATTCTCAAC+TGG 0.441332 2_1:+2737562 Msa0187920:CDS
AAAATGAATAGACTTGGAAA+TGG 0.442992 2_1:-2736202 None:intergenic
ACTTCAGGTCTTTGCTTGAA+AGG 0.470876 2_1:+2737697 Msa0187920:CDS
TATAAGGTTGCTTTCTAGAA+CGG 0.481854 2_1:-2736314 None:intergenic
GAGCTAACACGGCGTAAGAA+AGG 0.484665 2_1:-2737639 None:intergenic
ATATATATAAGTAAAGTAGT+AGG 0.491334 2_1:+2736425 Msa0187920:five_prime_UTR
AGTTTCCTTGAAGCATATGC+TGG 0.496553 2_1:+2737520 Msa0187920:CDS
ATTAAGAATATACAAACAAA+AGG 0.502484 2_1:+2736468 Msa0187920:five_prime_UTR
TCCTTAACTACTGAAACCCT+TGG 0.503695 2_1:+2736601 Msa0187920:CDS
GTCATCTCTACTATTCGTGC+CGG 0.513748 2_1:-2737608 None:intergenic
ATATATAAAATGAATAGACT+TGG 0.525533 2_1:-2736208 None:intergenic
TGTAATCAGATCATATGGTA+TGG 0.530296 2_1:+2737248 Msa0187920:intron
AGGAATTTACCATATTTCCA+TGG 0.534844 2_1:-2736654 None:intergenic
AGATGTTAGAAAATGGGAAA+GGG 0.535212 2_1:+2737920 Msa0187920:CDS
AAGGCATCATCCATTTCCTA+TGG 0.542944 2_1:-2737798 None:intergenic
TCTATATTTAGAAGCATCCA+TGG 0.553025 2_1:+2736637 Msa0187920:CDS
CAGATGTTAGAAAATGGGAA+AGG 0.557087 2_1:+2737919 Msa0187920:CDS
ATATTATCTATCAACTCAGC+CGG 0.573023 2_1:+2737589 Msa0187920:CDS
GGATACTTAATTCTGACCAT+AGG 0.582839 2_1:+2737782 Msa0187920:CDS
AGAGCTTTGAACACAACCAA+GGG 0.600437 2_1:-2736617 None:intergenic
AGATCATATGGTATGGAGCA+TGG 0.601542 2_1:+2737255 Msa0187920:intron
AAAGGATATGGCATGTTCTG+TGG 0.607383 2_1:+2736486 Msa0187920:exon
CTTGGAAATGGAACGTAAAG+CGG 0.619711 2_1:-2736190 None:intergenic
ACAGAAATCAATGTTAACCA+AGG 0.624182 2_1:+2737468 Msa0187920:intron
TAGAGCTTTGAACACAACCA+AGG 0.633504 2_1:-2736618 None:intergenic
GTTAACCAAGGTATCAACCA+TGG 0.651380 2_1:+2737480 Msa0187920:CDS
TATGCTGGAGAATCAAGAGA+TGG 0.654209 2_1:+2737535 Msa0187920:CDS
TGACCTGAAAAGAGCTAACA+CGG 0.676515 2_1:-2737650 None:intergenic
TAGTTGTGTGCATTCATGTG+TGG 0.691763 2_1:+2737734 Msa0187920:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTATTATCTG+TGG + chr2_1:2736224-2736243 Msa0187920:five_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG - chr2_1:2737429-2737448 None:intergenic 10.0%
!!! TATATATTTTTTATTATCTG+TGG + chr2_1:2736224-2736243 Msa0187920:five_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG - chr2_1:2737429-2737448 None:intergenic 10.0%
!! ATATATAAAATGAATAGACT+TGG - chr2_1:2736211-2736230 None:intergenic 15.0%
!! ATATATATAAGTAAAGTAGT+AGG + chr2_1:2736425-2736444 Msa0187920:five_prime_UTR 15.0%
!! ATTAAGAATATACAAACAAA+AGG + chr2_1:2736468-2736487 Msa0187920:five_prime_UTR 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG - chr2_1:2736698-2736717 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG + chr2_1:2736882-2736901 Msa0187920:intron 15.0%
!!! TACAATTTTTAATTCAAGTT+AGG - chr2_1:2737094-2737113 None:intergenic 15.0%
!! TTAGAATAAATTAAAATGGA+GGG - chr2_1:2737425-2737444 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG - chr2_1:2737426-2737445 None:intergenic 15.0%
!! TGAAAGAATGAATAAATATT+AGG + chr2_1:2738085-2738104 Msa0187920:three_prime_UTR 15.0%
!! ATATATAAAATGAATAGACT+TGG - chr2_1:2736211-2736230 None:intergenic 15.0%
!! ATATATATAAGTAAAGTAGT+AGG + chr2_1:2736425-2736444 Msa0187920:five_prime_UTR 15.0%
!! ATTAAGAATATACAAACAAA+AGG + chr2_1:2736468-2736487 Msa0187920:five_prime_UTR 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG - chr2_1:2736698-2736717 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG + chr2_1:2736882-2736901 Msa0187920:intron 15.0%
!!! TACAATTTTTAATTCAAGTT+AGG - chr2_1:2737094-2737113 None:intergenic 15.0%
!! TTAGAATAAATTAAAATGGA+GGG - chr2_1:2737425-2737444 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG - chr2_1:2737426-2737445 None:intergenic 15.0%
!! AATATACAAACAAAAGGATA+TGG + chr2_1:2736474-2736493 Msa0187920:exon 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG + chr2_1:2737038-2737057 Msa0187920:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG + chr2_1:2737047-2737066 Msa0187920:intron 20.0%
!! AATATACAAACAAAAGGATA+TGG + chr2_1:2736474-2736493 Msa0187920:exon 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG + chr2_1:2737038-2737057 Msa0187920:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG + chr2_1:2737047-2737066 Msa0187920:intron 20.0%
! AAAATGAATAGACTTGGAAA+TGG - chr2_1:2736205-2736224 None:intergenic 25.0%
!!! AAATTTTGACTATGGTGTAA+AGG - chr2_1:2736290-2736309 None:intergenic 25.0%
! TTTACACCATAGTCAAAATT+TGG + chr2_1:2736289-2736308 Msa0187920:five_prime_UTR 25.0%
! TCATCTATAATCACAATTGT+TGG - chr2_1:2736556-2736575 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG + chr2_1:2736565-2736584 Msa0187920:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG - chr2_1:2736814-2736833 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG + chr2_1:2736997-2737016 Msa0187920:intron 25.0%
!! TCATGTTTGGATTCAATTTT+GGG + chr2_1:2736998-2737017 Msa0187920:intron 25.0%
! TTTCATGTAATCAGATCATA+TGG + chr2_1:2737243-2737262 Msa0187920:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG - chr2_1:2737500-2737519 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG + chr2_1:2737914-2737933 Msa0187920:CDS 25.0%
! AAAATGAATAGACTTGGAAA+TGG - chr2_1:2736205-2736224 None:intergenic 25.0%
!!! AAATTTTGACTATGGTGTAA+AGG - chr2_1:2736290-2736309 None:intergenic 25.0%
! TTTACACCATAGTCAAAATT+TGG + chr2_1:2736289-2736308 Msa0187920:five_prime_UTR 25.0%
! TCATCTATAATCACAATTGT+TGG - chr2_1:2736556-2736575 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG + chr2_1:2736565-2736584 Msa0187920:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG - chr2_1:2736814-2736833 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG + chr2_1:2736997-2737016 Msa0187920:intron 25.0%
!! TCATGTTTGGATTCAATTTT+GGG + chr2_1:2736998-2737017 Msa0187920:intron 25.0%
! TTTCATGTAATCAGATCATA+TGG + chr2_1:2737243-2737262 Msa0187920:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG - chr2_1:2737500-2737519 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG + chr2_1:2737914-2737933 Msa0187920:CDS 25.0%
GAAACAAAAACAAACAACAG+TGG + chr2_1:2736255-2736274 Msa0187920:five_prime_UTR 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG - chr2_1:2736317-2736336 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG - chr2_1:2736376-2736395 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG + chr2_1:2736507-2736526 Msa0187920:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG + chr2_1:2736637-2736656 Msa0187920:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG - chr2_1:2736657-2736676 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG - chr2_1:2736774-2736793 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG - chr2_1:2736775-2736794 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG - chr2_1:2736823-2736842 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG + chr2_1:2736829-2736848 Msa0187920:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG + chr2_1:2736921-2736940 Msa0187920:intron 30.0%
TGTAATCAGATCATATGGTA+TGG + chr2_1:2737248-2737267 Msa0187920:intron 30.0%
ATGCATACAAAACATCTGAA+AGG - chr2_1:2737344-2737363 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG - chr2_1:2737410-2737429 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG + chr2_1:2737468-2737487 Msa0187920:intron 30.0%
ATATTATCTATCAACTCAGC+CGG + chr2_1:2737589-2737608 Msa0187920:CDS 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG + chr2_1:2737761-2737780 Msa0187920:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG + chr2_1:2737788-2737807 Msa0187920:CDS 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG - chr2_1:2737820-2737839 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG + chr2_1:2737859-2737878 Msa0187920:CDS 30.0%
CATCATCAGATGTTAGAAAA+TGG + chr2_1:2737913-2737932 Msa0187920:CDS 30.0%
AGATGTTAGAAAATGGGAAA+GGG + chr2_1:2737920-2737939 Msa0187920:CDS 30.0%
AGTGAAAAATAACTTAGCAG+AGG - chr2_1:2738014-2738033 None:intergenic 30.0%
GAAACAAAAACAAACAACAG+TGG + chr2_1:2736255-2736274 Msa0187920:five_prime_UTR 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG - chr2_1:2736317-2736336 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG - chr2_1:2736376-2736395 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG + chr2_1:2736507-2736526 Msa0187920:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG + chr2_1:2736637-2736656 Msa0187920:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG - chr2_1:2736657-2736676 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG - chr2_1:2736774-2736793 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG - chr2_1:2736775-2736794 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG - chr2_1:2736823-2736842 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG + chr2_1:2736829-2736848 Msa0187920:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG + chr2_1:2736921-2736940 Msa0187920:intron 30.0%
TGTAATCAGATCATATGGTA+TGG + chr2_1:2737248-2737267 Msa0187920:intron 30.0%
ATGCATACAAAACATCTGAA+AGG - chr2_1:2737344-2737363 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG - chr2_1:2737410-2737429 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG + chr2_1:2737468-2737487 Msa0187920:intron 30.0%
ATATTATCTATCAACTCAGC+CGG + chr2_1:2737589-2737608 Msa0187920:CDS 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG + chr2_1:2737761-2737780 Msa0187920:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG + chr2_1:2737788-2737807 Msa0187920:CDS 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG - chr2_1:2737820-2737839 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG + chr2_1:2737859-2737878 Msa0187920:CDS 30.0%
CATCATCAGATGTTAGAAAA+TGG + chr2_1:2737913-2737932 Msa0187920:CDS 30.0%
AGATGTTAGAAAATGGGAAA+GGG + chr2_1:2737920-2737939 Msa0187920:CDS 30.0%
AGTGAAAAATAACTTAGCAG+AGG - chr2_1:2738014-2738033 None:intergenic 30.0%
!!! ACGGTTCCAAATTTTGACTA+TGG - chr2_1:2736298-2736317 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG + chr2_1:2736645-2736664 Msa0187920:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG - chr2_1:2736677-2736696 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG - chr2_1:2736991-2737010 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG - chr2_1:2737196-2737215 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG + chr2_1:2737274-2737293 Msa0187920:CDS 35.0%
! AAAATAGAAGGCATGACTTC+AGG + chr2_1:2737682-2737701 Msa0187920:CDS 35.0%
ACATGAATGCACACAACTAT+TGG - chr2_1:2737733-2737752 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG + chr2_1:2737782-2737801 Msa0187920:CDS 35.0%
CAGATGTTAGAAAATGGGAA+AGG + chr2_1:2737919-2737938 Msa0187920:CDS 35.0%
!!! ACGGTTCCAAATTTTGACTA+TGG - chr2_1:2736298-2736317 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG + chr2_1:2736645-2736664 Msa0187920:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG - chr2_1:2736677-2736696 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG - chr2_1:2736991-2737010 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG - chr2_1:2737196-2737215 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG + chr2_1:2737274-2737293 Msa0187920:CDS 35.0%
! AAAATAGAAGGCATGACTTC+AGG + chr2_1:2737682-2737701 Msa0187920:CDS 35.0%
ACATGAATGCACACAACTAT+TGG - chr2_1:2737733-2737752 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG + chr2_1:2737782-2737801 Msa0187920:CDS 35.0%
CAGATGTTAGAAAATGGGAA+AGG + chr2_1:2737919-2737938 Msa0187920:CDS 35.0%
CTTGGAAATGGAACGTAAAG+CGG - chr2_1:2736193-2736212 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG - chr2_1:2736333-2736352 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG + chr2_1:2736486-2736505 Msa0187920:exon 40.0%
TCCTTAACTACTGAAACCCT+TGG + chr2_1:2736601-2736620 Msa0187920:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG - chr2_1:2736605-2736624 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG - chr2_1:2736620-2736639 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG - chr2_1:2736621-2736640 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG + chr2_1:2736836-2736855 Msa0187920:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG - chr2_1:2736957-2736976 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG - chr2_1:2737124-2737143 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG + chr2_1:2737255-2737274 Msa0187920:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG + chr2_1:2737480-2737499 Msa0187920:CDS 40.0%
!! TTTTGCCATGGTTGATACCT+TGG - chr2_1:2737488-2737507 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG + chr2_1:2737520-2737539 Msa0187920:CDS 40.0%
ATTCTCCAGCATATGCTTCA+AGG - chr2_1:2737528-2737547 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr2_1:2737535-2737554 Msa0187920:CDS 40.0%
TCAGCTGCAAAATTCTCAAC+TGG + chr2_1:2737562-2737581 Msa0187920:CDS 40.0%
TGACCTGAAAAGAGCTAACA+CGG - chr2_1:2737653-2737672 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG + chr2_1:2737670-2737689 Msa0187920:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG + chr2_1:2737697-2737716 Msa0187920:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG + chr2_1:2737734-2737753 Msa0187920:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG - chr2_1:2737801-2737820 None:intergenic 40.0%
CTTGGAAATGGAACGTAAAG+CGG - chr2_1:2736193-2736212 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG - chr2_1:2736333-2736352 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG + chr2_1:2736486-2736505 Msa0187920:exon 40.0%
TCCTTAACTACTGAAACCCT+TGG + chr2_1:2736601-2736620 Msa0187920:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG - chr2_1:2736605-2736624 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG - chr2_1:2736620-2736639 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG - chr2_1:2736621-2736640 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG + chr2_1:2736836-2736855 Msa0187920:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG - chr2_1:2736957-2736976 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG - chr2_1:2737124-2737143 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG + chr2_1:2737255-2737274 Msa0187920:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG + chr2_1:2737480-2737499 Msa0187920:CDS 40.0%
!! TTTTGCCATGGTTGATACCT+TGG - chr2_1:2737488-2737507 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG + chr2_1:2737520-2737539 Msa0187920:CDS 40.0%
ATTCTCCAGCATATGCTTCA+AGG - chr2_1:2737528-2737547 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG + chr2_1:2737535-2737554 Msa0187920:CDS 40.0%
TCAGCTGCAAAATTCTCAAC+TGG + chr2_1:2737562-2737581 Msa0187920:CDS 40.0%
TGACCTGAAAAGAGCTAACA+CGG - chr2_1:2737653-2737672 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG + chr2_1:2737670-2737689 Msa0187920:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG + chr2_1:2737697-2737716 Msa0187920:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG + chr2_1:2737734-2737753 Msa0187920:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG - chr2_1:2737801-2737820 None:intergenic 40.0%
AGTCGAGTTTGCGTGTCTTA+TGG + chr2_1:2736341-2736360 Msa0187920:five_prime_UTR 45.0%
CATGAACTACCATGGAGCAA+TGG - chr2_1:2736983-2737002 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG + chr2_1:2736985-2737004 Msa0187920:intron 45.0%
GTCATCTCTACTATTCGTGC+CGG - chr2_1:2737611-2737630 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG - chr2_1:2737633-2737652 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG + chr2_1:2737659-2737678 Msa0187920:CDS 45.0%
AGTCGAGTTTGCGTGTCTTA+TGG + chr2_1:2736341-2736360 Msa0187920:five_prime_UTR 45.0%
CATGAACTACCATGGAGCAA+TGG - chr2_1:2736983-2737002 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG + chr2_1:2736985-2737004 Msa0187920:intron 45.0%
GTCATCTCTACTATTCGTGC+CGG - chr2_1:2737611-2737630 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG - chr2_1:2737633-2737652 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG + chr2_1:2737659-2737678 Msa0187920:CDS 45.0%
CTTCAGATGCCATTGCTCCA+TGG + chr2_1:2736971-2736990 Msa0187920:intron 50.0%
GAGCTAACACGGCGTAAGAA+AGG - chr2_1:2737642-2737661 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG + chr2_1:2737647-2737666 Msa0187920:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG + chr2_1:2737658-2737677 Msa0187920:CDS 50.0%
CTTCAGATGCCATTGCTCCA+TGG + chr2_1:2736971-2736990 Msa0187920:intron 50.0%
GAGCTAACACGGCGTAAGAA+AGG - chr2_1:2737642-2737661 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG + chr2_1:2737647-2737666 Msa0187920:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG + chr2_1:2737658-2737677 Msa0187920:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 2736166 2738106 2736166 ID=Msa0187920;Name=Msa0187920
chr2_1 mRNA 2736166 2738106 2736166 ID=Msa0187920-mRNA-1;Parent=Msa0187920;Name=Msa0187920-mRNA-1;_AED=0.08;_eAED=0.08;_QI=327|1|1|1|1|1|3|134|246
chr2_1 exon 2736166 2736666 2736166 ID=Msa0187920-mRNA-1:exon:1241;Parent=Msa0187920-mRNA-1
chr2_1 exon 2737257 2737322 2737257 ID=Msa0187920-mRNA-1:exon:1242;Parent=Msa0187920-mRNA-1
chr2_1 exon 2737472 2738106 2737472 ID=Msa0187920-mRNA-1:exon:1243;Parent=Msa0187920-mRNA-1
chr2_1 five_prime_UTR 2736166 2736492 2736166 ID=Msa0187920-mRNA-1:five_prime_utr;Parent=Msa0187920-mRNA-1
chr2_1 CDS 2736493 2736666 2736493 ID=Msa0187920-mRNA-1:cds;Parent=Msa0187920-mRNA-1
chr2_1 CDS 2737257 2737322 2737257 ID=Msa0187920-mRNA-1:cds;Parent=Msa0187920-mRNA-1
chr2_1 CDS 2737472 2737972 2737472 ID=Msa0187920-mRNA-1:cds;Parent=Msa0187920-mRNA-1
chr2_1 three_prime_UTR 2737973 2738106 2737973 ID=Msa0187920-mRNA-1:three_prime_utr;Parent=Msa0187920-mRNA-1
Gene Sequence

>Msa0187920

ATGGCATGTTCTGTGGCTTTTAACAACAAGAATTTGGAAATAAGTTTCTTTGTTTTCAAGCCAACAATTGTGATTATAGATGATCTTGTTCATGGTCTCAAACAATTCTCCTTAACTACTGAAACCCTTGGTTGTGTTCAAAGCTCTATATTTAGAAGCATCCATGGAAATATGATCATATGGTATGGAGCATGGCAGAAGCAATCTATTAAGGAAAAAGAGCTTCTAACTTCAACTCTTAAATCAATGTTAACCAAGGTATCAACCATGGCAAAATTAATTGAACATAGTTTCCTTGAAGCATATGCTGGAGAATCAAGAGATGGCTCATCAGCTGCAAAATTCTCAACTGGAGACATATTATCTATCAACTCAGCCGGCACGAATAGTAGAGATGACCTTAATGACCTTTCTTACGCCGTGTTAGCTCTTTTCAGGTCACGCTTTGGGAAAATAGAAGGCATGACTTCAGGTCTTTGCTTGAAAGGACAAAGCAGACCAATAGTTGTGTGCATTCATGTGTGGAAATCTCTTCATTTTTGTTACTCATGGATACTTAATTCTGACCATAGGAAATGGATGATGCCTTATCTTGAAAAATTCGCGATCGATATGAAGTATGATATTTTCAAAGTAGTGTATGTTAGTGGTGACAATGTTGTTGATGTTAACTATATTTCTCATCATCAGATGTTAGAAAATGGGAAAGGGAGCATAGAAAGACAGATAATGCAGAACTGA
Protein sequence

>Msa0187920

MACSVAFNNKNLEISFFVFKPTIVIIDDLVHGLKQFSLTTETLGCVQSSIFRSIHGNMIIWYGAWQKQSIKEKELLTSTLKSMLTKVSTMAKLIEHSFLEAYAGESRDGSSAAKFSTGDILSINSAGTNSRDDLNDLSYAVLALFRSRFGKIEGMTSGLCLKGQSRPIVVCIHVWKSLHFCYSWILNSDHRKWMMPYLEKFAIDMKYDIFKVVYVSGDNVVDVNYISHHQMLENGKGSIERQIMQN*