AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0299000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0299000 MtrunA17_Chr2g0285421 97.561 246 6 0 1 246 1 246 0.0 501
Msa0299000 MtrunA17_Chr8g0376721 65.726 248 82 3 1 246 2 248 2.54e-118 338
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 51 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTACACCATAGTCAAATT+TGG 0.164582 2_3:-53325331 Msa0299000:five_prime_UTR
CATCATCAGATGTTAGAAAA+TGG 0.212688 2_3:-53323706 Msa0299000:CDS
TCATTTGAGAGTGTTCAAAT+TGG 0.324071 2_3:+53325247 None:intergenic
TAGAAGCATCCATGGAAATA+TGG 0.347628 2_3:-53324976 Msa0299000:intron
ATGGCAGAAGCAATCTATTA+AGG 0.348198 2_3:-53324345 Msa0299000:CDS
AACGGTTCCAAATTTGACTA+TGG 0.350338 2_3:+53325324 None:intergenic
AGTCGAGTTTGCGTGTCTTA+TGG 0.356753 2_3:-53325279 Msa0299000:five_prime_UTR
AAAATAGAAGGCATGACTTC+AGG 0.371700 2_3:-53323937 Msa0299000:CDS
AATATACAAACAAAAGGATA+TGG 0.372931 2_3:-53325147 Msa0299000:exon
ACATGAATGCACACAACTAT+TGG 0.377348 2_3:+53323889 None:intergenic
ATTATAGATGATCTTGTTCA+TGG 0.379410 2_3:-53325056 Msa0299000:CDS
TATAGTAGAAGTAAAGTAGT+AGG 0.380385 2_3:-53325196 Msa0299000:five_prime_UTR
TTTCATGTAATCAGATCATA+TGG 0.381243 2_3:-53324376 Msa0299000:intron
TTCAAAGTAGTGTATGTTAG+TGG 0.388260 2_3:-53323760 Msa0299000:CDS
ACCAAGGGTTTCAGTAGTTA+AGG 0.397101 2_3:+53325019 None:intergenic
ATTCTCCAGCATATGCTTCA+AGG 0.397395 2_3:+53324094 None:intergenic
TCATCTATAATCACAATTGT+TGG 0.398129 2_3:+53325068 None:intergenic
GCAAACTCGACTCTTCTATA+AGG 0.418674 2_3:+53325290 None:intergenic
ATCATCAGATGTTAGAAAAT+GGG 0.425313 2_3:-53323705 Msa0299000:CDS
TTAATTCTGACCATAGGAAA+TGG 0.426007 2_3:-53323831 Msa0299000:CDS
TCACGCTTTGGGAAAATAGA+AGG 0.429485 2_3:-53323949 Msa0299000:CDS
CGGCGTAAGAAAGGTCATTA+AGG 0.433721 2_3:+53323989 None:intergenic
TCAGCTGCAAAATTCTCAAC+TGG 0.441332 2_3:-53324057 Msa0299000:CDS
AAAATGAATAGACTTGGAAA+TGG 0.442992 2_3:+53325417 None:intergenic
CAAATTTGACTATGGTGTAA+AGG 0.456825 2_3:+53325332 None:intergenic
ACTTCAGGTCTTTGCTTGAA+AGG 0.470876 2_3:-53323922 Msa0299000:CDS
TATAAGGTTGCTTTCTAGAA+CGG 0.481854 2_3:+53325306 None:intergenic
GAGCTAACACGGCGTAAGAA+AGG 0.484665 2_3:+53323980 None:intergenic
AGTTTCCTTGAAGCATATGC+TGG 0.496553 2_3:-53324099 Msa0299000:CDS
ATTAAGAATATACAAACAAA+AGG 0.502484 2_3:-53325153 Msa0299000:five_prime_UTR
TCCTTAACTACTGAAACCCT+TGG 0.503695 2_3:-53325020 Msa0299000:CDS
GTCATCTCTACTATTCGTGC+CGG 0.513748 2_3:+53324011 None:intergenic
ATATATAAAATGAATAGACT+TGG 0.525533 2_3:+53325411 None:intergenic
TGTAATCAGATCATATGGTA+TGG 0.530296 2_3:-53324371 Msa0299000:intron
AGGAATTTACCATATTTCCA+TGG 0.534844 2_3:+53324967 None:intergenic
AGATGTTAGAAAATGGGAAA+GGG 0.535212 2_3:-53323699 Msa0299000:CDS
AAGGCATCATCCATTTCCTA+TGG 0.542944 2_3:+53323821 None:intergenic
TCTATATTTAGAAGCATCCA+TGG 0.553025 2_3:-53324984 Msa0299000:CDS
CAGATGTTAGAAAATGGGAA+AGG 0.557087 2_3:-53323700 Msa0299000:CDS
ATATTATCTATCAACTCAGC+CGG 0.573023 2_3:-53324030 Msa0299000:CDS
GGATACTTAATTCTGACCAT+AGG 0.582839 2_3:-53323837 Msa0299000:CDS
AGAGCTTTGAACACAACCAA+GGG 0.600437 2_3:+53325004 None:intergenic
AGATCATATGGTATGGAGCA+TGG 0.601542 2_3:-53324364 Msa0299000:CDS
AAAGGATATGGCATGTTCTG+TGG 0.607383 2_3:-53325135 Msa0299000:exon
CTTGGAAATGGAACGTAAAG+CGG 0.619711 2_3:+53325429 None:intergenic
ACAGAAATCAATGTTAACCA+AGG 0.624182 2_3:-53324151 Msa0299000:intron
TAGAGCTTTGAACACAACCA+AGG 0.633504 2_3:+53325003 None:intergenic
GTTAACCAAGGTATCAACCA+TGG 0.651380 2_3:-53324139 Msa0299000:CDS
TATGCTGGAGAATCAAGAGA+TGG 0.654209 2_3:-53324084 Msa0299000:CDS
TGACCTGAAAAGAGCTAACA+CGG 0.676515 2_3:+53323969 None:intergenic
TAGTTGTGTGCATTCATGTG+TGG 0.691763 2_3:-53323885 Msa0299000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTATTATCTG+TGG - chr2_3:53323593-53323612 Msa0299000:three_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG + chr2_3:53324798-53324817 None:intergenic 10.0%
!!! TATATATTTTTTATTATCTG+TGG - chr2_3:53323593-53323612 Msa0299000:three_prime_UTR 10.0%
!! TGTATTAGAATAAATTAAAA+TGG + chr2_3:53324798-53324817 None:intergenic 10.0%
!! ATATATAAAATGAATAGACT+TGG + chr2_3:53323580-53323599 None:intergenic 15.0%
!! ATTAAGAATATACAAACAAA+AGG - chr2_3:53323835-53323854 Msa0299000:CDS 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG + chr2_3:53324065-53324084 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG - chr2_3:53324249-53324268 Msa0299000:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG + chr2_3:53324794-53324813 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG + chr2_3:53324795-53324814 None:intergenic 15.0%
!! TGAAAGAATGAATAAATATT+AGG - chr2_3:53325454-53325473 Msa0299000:five_prime_UTR 15.0%
!! ATATATAAAATGAATAGACT+TGG + chr2_3:53323580-53323599 None:intergenic 15.0%
!! ATTAAGAATATACAAACAAA+AGG - chr2_3:53323835-53323854 Msa0299000:CDS 15.0%
!! AGTAAAATTGAATTGAAAAT+TGG + chr2_3:53324065-53324084 None:intergenic 15.0%
!! CATATAATATCATCTTTATT+TGG - chr2_3:53324249-53324268 Msa0299000:intron 15.0%
!! TTAGAATAAATTAAAATGGA+GGG + chr2_3:53324794-53324813 None:intergenic 15.0%
!! ATTAGAATAAATTAAAATGG+AGG + chr2_3:53324795-53324814 None:intergenic 15.0%
!! AATATACAAACAAAAGGATA+TGG - chr2_3:53323841-53323860 Msa0299000:CDS 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG - chr2_3:53324405-53324424 Msa0299000:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG - chr2_3:53324414-53324433 Msa0299000:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG + chr2_3:53324461-53324480 None:intergenic 20.0%
!! AATATACAAACAAAAGGATA+TGG - chr2_3:53323841-53323860 Msa0299000:CDS 20.0%
!!! TGAAGACTAAAAAATGATTT+TGG - chr2_3:53324405-53324424 Msa0299000:intron 20.0%
!!! AAAAATGATTTTGGTATGTT+TGG - chr2_3:53324414-53324433 Msa0299000:intron 20.0%
!!! ACAATTTTTAACTTCAAGTT+AGG + chr2_3:53324461-53324480 None:intergenic 20.0%
! AAAATGAATAGACTTGGAAA+TGG + chr2_3:53323574-53323593 None:intergenic 25.0%
! TATAGTAGAAGTAAAGTAGT+AGG - chr2_3:53323792-53323811 Msa0299000:CDS 25.0%
! TCATCTATAATCACAATTGT+TGG + chr2_3:53323923-53323942 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG - chr2_3:53323932-53323951 Msa0299000:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG + chr2_3:53324181-53324200 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG - chr2_3:53324364-53324383 Msa0299000:CDS 25.0%
!! TCATGTTTGGATTCAATTTT+GGG - chr2_3:53324365-53324384 Msa0299000:CDS 25.0%
! TTTCATGTAATCAGATCATA+TGG - chr2_3:53324612-53324631 Msa0299000:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG + chr2_3:53324869-53324888 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG - chr2_3:53325283-53325302 Msa0299000:five_prime_UTR 25.0%
! AAAATGAATAGACTTGGAAA+TGG + chr2_3:53323574-53323593 None:intergenic 25.0%
! TATAGTAGAAGTAAAGTAGT+AGG - chr2_3:53323792-53323811 Msa0299000:CDS 25.0%
! TCATCTATAATCACAATTGT+TGG + chr2_3:53323923-53323942 None:intergenic 25.0%
! ATTATAGATGATCTTGTTCA+TGG - chr2_3:53323932-53323951 Msa0299000:CDS 25.0%
! TAAATTCACAATGGAAGAAA+TGG + chr2_3:53324181-53324200 None:intergenic 25.0%
!! TTCATGTTTGGATTCAATTT+TGG - chr2_3:53324364-53324383 Msa0299000:CDS 25.0%
!! TCATGTTTGGATTCAATTTT+GGG - chr2_3:53324365-53324384 Msa0299000:CDS 25.0%
! TTTCATGTAATCAGATCATA+TGG - chr2_3:53324612-53324631 Msa0299000:intron 25.0%
!! ATGTTCAATTAATTTTGCCA+TGG + chr2_3:53324869-53324888 None:intergenic 25.0%
! ATCATCAGATGTTAGAAAAT+GGG - chr2_3:53325283-53325302 Msa0299000:five_prime_UTR 25.0%
GAAACAAAAACAAACAACAG+TGG - chr2_3:53323624-53323643 Msa0299000:three_prime_UTR 30.0%
CAAATTTGACTATGGTGTAA+AGG + chr2_3:53323659-53323678 None:intergenic 30.0%
CTTTACACCATAGTCAAATT+TGG - chr2_3:53323657-53323676 Msa0299000:exon 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG + chr2_3:53323685-53323704 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG + chr2_3:53323744-53323763 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG - chr2_3:53323874-53323893 Msa0299000:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG - chr2_3:53324004-53324023 Msa0299000:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG + chr2_3:53324024-53324043 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG + chr2_3:53324141-53324160 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG + chr2_3:53324142-53324161 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG + chr2_3:53324190-53324209 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG - chr2_3:53324196-53324215 Msa0299000:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG - chr2_3:53324288-53324307 Msa0299000:intron 30.0%
TGTAATCAGATCATATGGTA+TGG - chr2_3:53324617-53324636 Msa0299000:intron 30.0%
ATGCATACAAAACATCTGAA+AGG + chr2_3:53324713-53324732 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG + chr2_3:53324779-53324798 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG - chr2_3:53324837-53324856 Msa0299000:intron 30.0%
ATATTATCTATCAACTCAGC+CGG - chr2_3:53324958-53324977 Msa0299000:intron 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG - chr2_3:53325130-53325149 Msa0299000:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG - chr2_3:53325157-53325176 Msa0299000:five_prime_UTR 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG + chr2_3:53325189-53325208 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG - chr2_3:53325228-53325247 Msa0299000:five_prime_UTR 30.0%
CATCATCAGATGTTAGAAAA+TGG - chr2_3:53325282-53325301 Msa0299000:five_prime_UTR 30.0%
AGATGTTAGAAAATGGGAAA+GGG - chr2_3:53325289-53325308 Msa0299000:five_prime_UTR 30.0%
AGTGAAAAATAACTTAGCAG+AGG + chr2_3:53325383-53325402 None:intergenic 30.0%
GAAACAAAAACAAACAACAG+TGG - chr2_3:53323624-53323643 Msa0299000:three_prime_UTR 30.0%
CAAATTTGACTATGGTGTAA+AGG + chr2_3:53323659-53323678 None:intergenic 30.0%
CTTTACACCATAGTCAAATT+TGG - chr2_3:53323657-53323676 Msa0299000:exon 30.0%
! TATAAGGTTGCTTTCTAGAA+CGG + chr2_3:53323685-53323704 None:intergenic 30.0%
!! TCATTTGAGAGTGTTCAAAT+TGG + chr2_3:53323744-53323763 None:intergenic 30.0%
! GGCTTTTAACAACAAGAATT+TGG - chr2_3:53323874-53323893 Msa0299000:CDS 30.0%
!! TCTATATTTAGAAGCATCCA+TGG - chr2_3:53324004-53324023 Msa0299000:CDS 30.0%
AGGAATTTACCATATTTCCA+TGG + chr2_3:53324024-53324043 None:intergenic 30.0%
CAGCAAAGTTTCAAACAAAA+GGG + chr2_3:53324141-53324160 None:intergenic 30.0%
TCAGCAAAGTTTCAAACAAA+AGG + chr2_3:53324142-53324161 None:intergenic 30.0%
TTTGCAGCATAAATTCACAA+TGG + chr2_3:53324190-53324209 None:intergenic 30.0%
! ATTTATGCTGCAAATTTGCT+TGG - chr2_3:53324196-53324215 Msa0299000:intron 30.0%
!! TCATCAAAAGTTTAGTTTGC+TGG - chr2_3:53324288-53324307 Msa0299000:intron 30.0%
TGTAATCAGATCATATGGTA+TGG - chr2_3:53324617-53324636 Msa0299000:intron 30.0%
ATGCATACAAAACATCTGAA+AGG + chr2_3:53324713-53324732 None:intergenic 30.0%
ATGGAGGGAAATAATTGATT+TGG + chr2_3:53324779-53324798 None:intergenic 30.0%
ACAGAAATCAATGTTAACCA+AGG - chr2_3:53324837-53324856 Msa0299000:intron 30.0%
ATATTATCTATCAACTCAGC+CGG - chr2_3:53324958-53324977 Msa0299000:intron 30.0%
!! CTCTTCATTTTTGTTACTCA+TGG - chr2_3:53325130-53325149 Msa0299000:CDS 30.0%
TTAATTCTGACCATAGGAAA+TGG - chr2_3:53325157-53325176 Msa0299000:five_prime_UTR 30.0%
!!! ATCGCGAATTTTTCAAGATA+AGG + chr2_3:53325189-53325208 None:intergenic 30.0%
TTCAAAGTAGTGTATGTTAG+TGG - chr2_3:53325228-53325247 Msa0299000:five_prime_UTR 30.0%
CATCATCAGATGTTAGAAAA+TGG - chr2_3:53325282-53325301 Msa0299000:five_prime_UTR 30.0%
AGATGTTAGAAAATGGGAAA+GGG - chr2_3:53325289-53325308 Msa0299000:five_prime_UTR 30.0%
AGTGAAAAATAACTTAGCAG+AGG + chr2_3:53325383-53325402 None:intergenic 30.0%
AACGGTTCCAAATTTGACTA+TGG + chr2_3:53323667-53323686 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG - chr2_3:53324012-53324031 Msa0299000:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG + chr2_3:53324044-53324063 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG + chr2_3:53324358-53324377 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG + chr2_3:53324565-53324584 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG - chr2_3:53324643-53324662 Msa0299000:intron 35.0%
! AAAATAGAAGGCATGACTTC+AGG - chr2_3:53325051-53325070 Msa0299000:CDS 35.0%
ACATGAATGCACACAACTAT+TGG + chr2_3:53325102-53325121 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG - chr2_3:53325151-53325170 Msa0299000:five_prime_UTR 35.0%
CAGATGTTAGAAAATGGGAA+AGG - chr2_3:53325288-53325307 Msa0299000:five_prime_UTR 35.0%
AACGGTTCCAAATTTGACTA+TGG + chr2_3:53323667-53323686 None:intergenic 35.0%
!! TAGAAGCATCCATGGAAATA+TGG - chr2_3:53324012-53324031 Msa0299000:CDS 35.0%
GGAAATCAGAGAAAGAAAAC+AGG + chr2_3:53324044-53324063 None:intergenic 35.0%
AATCCAAACATGAACTACCA+TGG + chr2_3:53324358-53324377 None:intergenic 35.0%
!! ACAATGCGTGTTTGTTTCTA+CGG + chr2_3:53324565-53324584 None:intergenic 35.0%
ATGGCAGAAGCAATCTATTA+AGG - chr2_3:53324643-53324662 Msa0299000:intron 35.0%
! AAAATAGAAGGCATGACTTC+AGG - chr2_3:53325051-53325070 Msa0299000:CDS 35.0%
ACATGAATGCACACAACTAT+TGG + chr2_3:53325102-53325121 None:intergenic 35.0%
GGATACTTAATTCTGACCAT+AGG - chr2_3:53325151-53325170 Msa0299000:five_prime_UTR 35.0%
CAGATGTTAGAAAATGGGAA+AGG - chr2_3:53325288-53325307 Msa0299000:five_prime_UTR 35.0%
CTTGGAAATGGAACGTAAAG+CGG + chr2_3:53323562-53323581 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG + chr2_3:53323701-53323720 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG - chr2_3:53323853-53323872 Msa0299000:CDS 40.0%
TCCTTAACTACTGAAACCCT+TGG - chr2_3:53323968-53323987 Msa0299000:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG + chr2_3:53323972-53323991 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG + chr2_3:53323987-53324006 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG + chr2_3:53323988-53324007 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG - chr2_3:53324203-53324222 Msa0299000:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG + chr2_3:53324324-53324343 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG + chr2_3:53324492-53324511 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG - chr2_3:53324624-53324643 Msa0299000:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG - chr2_3:53324849-53324868 Msa0299000:intron 40.0%
!! TTTTGCCATGGTTGATACCT+TGG + chr2_3:53324857-53324876 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG - chr2_3:53324889-53324908 Msa0299000:intron 40.0%
ATTCTCCAGCATATGCTTCA+AGG + chr2_3:53324897-53324916 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG - chr2_3:53324904-53324923 Msa0299000:intron 40.0%
TCAGCTGCAAAATTCTCAAC+TGG - chr2_3:53324931-53324950 Msa0299000:intron 40.0%
TGACCTGAAAAGAGCTAACA+CGG + chr2_3:53325022-53325041 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG - chr2_3:53325039-53325058 Msa0299000:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG - chr2_3:53325066-53325085 Msa0299000:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG - chr2_3:53325103-53325122 Msa0299000:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG + chr2_3:53325170-53325189 None:intergenic 40.0%
CTTGGAAATGGAACGTAAAG+CGG + chr2_3:53323562-53323581 None:intergenic 40.0%
GCAAACTCGACTCTTCTATA+AGG + chr2_3:53323701-53323720 None:intergenic 40.0%
AAAGGATATGGCATGTTCTG+TGG - chr2_3:53323853-53323872 Msa0299000:CDS 40.0%
TCCTTAACTACTGAAACCCT+TGG - chr2_3:53323968-53323987 Msa0299000:CDS 40.0%
ACCAAGGGTTTCAGTAGTTA+AGG + chr2_3:53323972-53323991 None:intergenic 40.0%
!! AGAGCTTTGAACACAACCAA+GGG + chr2_3:53323987-53324006 None:intergenic 40.0%
!! TAGAGCTTTGAACACAACCA+AGG + chr2_3:53323988-53324007 None:intergenic 40.0%
! CTGCAAATTTGCTTGGAAGT+TGG - chr2_3:53324203-53324222 Msa0299000:intron 40.0%
! CTGAAGTTGTCATTTGCTGA+AGG + chr2_3:53324324-53324343 None:intergenic 40.0%
! TGTGTAAAGCTCACGTTTTG+AGG + chr2_3:53324492-53324511 None:intergenic 40.0%
AGATCATATGGTATGGAGCA+TGG - chr2_3:53324624-53324643 Msa0299000:intron 40.0%
! GTTAACCAAGGTATCAACCA+TGG - chr2_3:53324849-53324868 Msa0299000:intron 40.0%
!! TTTTGCCATGGTTGATACCT+TGG + chr2_3:53324857-53324876 None:intergenic 40.0%
AGTTTCCTTGAAGCATATGC+TGG - chr2_3:53324889-53324908 Msa0299000:intron 40.0%
ATTCTCCAGCATATGCTTCA+AGG + chr2_3:53324897-53324916 None:intergenic 40.0%
TATGCTGGAGAATCAAGAGA+TGG - chr2_3:53324904-53324923 Msa0299000:intron 40.0%
TCAGCTGCAAAATTCTCAAC+TGG - chr2_3:53324931-53324950 Msa0299000:intron 40.0%
TGACCTGAAAAGAGCTAACA+CGG + chr2_3:53325022-53325041 None:intergenic 40.0%
!! TCACGCTTTGGGAAAATAGA+AGG - chr2_3:53325039-53325058 Msa0299000:CDS 40.0%
ACTTCAGGTCTTTGCTTGAA+AGG - chr2_3:53325066-53325085 Msa0299000:CDS 40.0%
TAGTTGTGTGCATTCATGTG+TGG - chr2_3:53325103-53325122 Msa0299000:CDS 40.0%
! AAGGCATCATCCATTTCCTA+TGG + chr2_3:53325170-53325189 None:intergenic 40.0%
AGTCGAGTTTGCGTGTCTTA+TGG - chr2_3:53323709-53323728 Msa0299000:CDS 45.0%
CATGAACTACCATGGAGCAA+TGG + chr2_3:53324350-53324369 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG - chr2_3:53324352-53324371 Msa0299000:CDS 45.0%
GTCATCTCTACTATTCGTGC+CGG + chr2_3:53324980-53324999 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG + chr2_3:53325002-53325021 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG - chr2_3:53325028-53325047 Msa0299000:CDS 45.0%
AGTCGAGTTTGCGTGTCTTA+TGG - chr2_3:53323709-53323728 Msa0299000:CDS 45.0%
CATGAACTACCATGGAGCAA+TGG + chr2_3:53324350-53324369 None:intergenic 45.0%
GCTCCATGGTAGTTCATGTT+TGG - chr2_3:53324352-53324371 Msa0299000:CDS 45.0%
GTCATCTCTACTATTCGTGC+CGG + chr2_3:53324980-53324999 None:intergenic 45.0%
CGGCGTAAGAAAGGTCATTA+AGG + chr2_3:53325002-53325021 None:intergenic 45.0%
!!! CTCTTTTCAGGTCACGCTTT+GGG - chr2_3:53325028-53325047 Msa0299000:CDS 45.0%
CTTCAGATGCCATTGCTCCA+TGG - chr2_3:53324338-53324357 Msa0299000:CDS 50.0%
GAGCTAACACGGCGTAAGAA+AGG + chr2_3:53325011-53325030 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG - chr2_3:53325016-53325035 Msa0299000:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG - chr2_3:53325027-53325046 Msa0299000:CDS 50.0%
CTTCAGATGCCATTGCTCCA+TGG - chr2_3:53324338-53324357 Msa0299000:CDS 50.0%
GAGCTAACACGGCGTAAGAA+AGG + chr2_3:53325011-53325030 None:intergenic 50.0%
!! ACGCCGTGTTAGCTCTTTTC+AGG - chr2_3:53325016-53325035 Msa0299000:CDS 50.0%
!!! GCTCTTTTCAGGTCACGCTT+TGG - chr2_3:53325027-53325046 Msa0299000:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 53323535 53325475 53323535 ID=Msa0299000;Name=Msa0299000
chr2_3 mRNA 53323535 53325475 53323535 ID=Msa0299000-mRNA-1;Parent=Msa0299000;Name=Msa0299000-mRNA-1;_AED=0.08;_eAED=0.08;_QI=325|1|1|1|1|1|3|134|246
chr2_3 exon 53323535 53324169 53323535 ID=Msa0299000-mRNA-1:exon:17136;Parent=Msa0299000-mRNA-1
chr2_3 exon 53324319 53324384 53324319 ID=Msa0299000-mRNA-1:exon:17135;Parent=Msa0299000-mRNA-1
chr2_3 exon 53324977 53325475 53324977 ID=Msa0299000-mRNA-1:exon:17134;Parent=Msa0299000-mRNA-1
chr2_3 five_prime_UTR 53325151 53325475 53325151 ID=Msa0299000-mRNA-1:five_prime_utr;Parent=Msa0299000-mRNA-1
chr2_3 CDS 53324977 53325150 53324977 ID=Msa0299000-mRNA-1:cds;Parent=Msa0299000-mRNA-1
chr2_3 CDS 53324319 53324384 53324319 ID=Msa0299000-mRNA-1:cds;Parent=Msa0299000-mRNA-1
chr2_3 CDS 53323669 53324169 53323669 ID=Msa0299000-mRNA-1:cds;Parent=Msa0299000-mRNA-1
chr2_3 three_prime_UTR 53323535 53323668 53323535 ID=Msa0299000-mRNA-1:three_prime_utr;Parent=Msa0299000-mRNA-1
Gene Sequence

>Msa0299000

ATGGCATGTTCTGTGGCTTTTAACAACAAGAATTTGGAAATAAGTTTCTTTGTTTTCAAGCCAACAATTGTGATTATAGATGATCTTGTTCATGGTCTCAAACAATTCTCCTTAACTACTGAAACCCTTGGTTGTGTTCAAAGCTCTATATTTAGAAGCATCCATGGAAATATGATCATATGGTATGGAGCATGGCAGAAGCAATCTATTAAGGAAAAAGAGCTTCTAACTTCAACTCTTAAATCAATGTTAACCAAGGTATCAACCATGGCAAAATTAATTGAACATAGTTTCCTTGAAGCATATGCTGGAGAATCAAGAGATGGCTCATCAGCTGCAAAATTCTCAACTGGAGACATATTATCTATCAACTCAGCCGGCACGAATAGTAGAGATGACCTTAATGACCTTTCTTACGCCGTGTTAGCTCTTTTCAGGTCACGCTTTGGGAAAATAGAAGGCATGACTTCAGGTCTTTGCTTGAAAGGACAAAGCAGACCAATAGTTGTGTGCATTCATGTGTGGAAATCTCTTCATTTTTGTTACTCATGGATACTTAATTCTGACCATAGGAAATGGATGATGCCTTATCTTGAAAAATTCGCGATCGATATGAAGTATGATATTTTCAAAGTAGTGTATGTTAGTGGTGACAATGTTGTTGATGTTAACTATATTTCTCATCATCAGATGTTAGAAAATGGGAAAGGGAGCATAGAAAGACAGATAATGCAGAACTGA
Protein sequence

>Msa0299000

MACSVAFNNKNLEISFFVFKPTIVIIDDLVHGLKQFSLTTETLGCVQSSIFRSIHGNMIIWYGAWQKQSIKEKELLTSTLKSMLTKVSTMAKLIEHSFLEAYAGESRDGSSAAKFSTGDILSINSAGTNSRDDLNDLSYAVLALFRSRFGKIEGMTSGLCLKGQSRPIVVCIHVWKSLHFCYSWILNSDHRKWMMPYLEKFAIDMKYDIFKVVYVSGDNVVDVNYISHHQMLENGKGSIERQIMQN*