AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0359370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0359370 MtrunA17_Chr1g0207381 87.243 243 24 2 2 237 13 255 4.62e-158 441
Msa0359370 MtrunA17_Chr4g0051721 87.845 181 22 0 2 182 13 193 1.04e-119 339
Msa0359370 MtrunA17_Chr3g0093841 29.032 155 107 2 4 158 20 171 1.75e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0359370 AT5G27260.1 44.444 153 84 1 3 155 9 160 3.71e-40 140
Msa0359370 AT2G29880.1 43.038 158 89 1 3 160 14 170 6.32e-40 140
Msa0359370 AT1G30140.2 32.889 225 137 5 2 222 8 222 4.10e-31 114
Msa0359370 AT1G30140.1 32.889 225 137 5 2 222 8 222 4.10e-31 114

Find 43 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCGTAACAACTCTGGTTT+TGG 0.286642 3_1:-46825814 Msa0359370:CDS
TGTTATGAATGCATCATTAT+TGG 0.292284 3_1:+46825452 None:intergenic
TACGGTGGTTCATAATTATC+TGG 0.363509 3_1:+46825429 None:intergenic
GAGCAAGAAAGTGAAAGTTT+TGG 0.364343 3_1:-46825498 Msa0359370:CDS
AGTATGGAGTAATTACTTAA+AGG 0.368835 3_1:-46825752 Msa0359370:intron
TCCGAGTACGAAAGGAGTTC+TGG 0.386489 3_1:-46825309 Msa0359370:CDS
TGAGTTGTTGTTCCTCTTGG+TGG 0.396694 3_1:-46826007 Msa0359370:CDS
GTTATGAATGCATCATTATT+GGG 0.403613 3_1:+46825453 None:intergenic
GAGTTCTAAACCACTTCATT+CGG 0.405087 3_1:+46825860 None:intergenic
GATTATTTGAAGATTCTTGT+TGG 0.439501 3_1:-46825588 Msa0359370:CDS
TTGAAGATTCTTGTTGGAGG+TGG 0.445118 3_1:-46825582 Msa0359370:CDS
GTTGGTTAGGTGATGGAGGT+TGG 0.445510 3_1:+46825406 None:intergenic
ACACGGCTACTGAAGAAGTA+TGG 0.447236 3_1:-46825768 Msa0359370:CDS
TAATGAGTTGTTGTTCCTCT+TGG 0.447268 3_1:-46826010 Msa0359370:CDS
AAAGGAGTTCTGGTGTTGTT+GGG 0.456514 3_1:-46825299 Msa0359370:CDS
GAAAGGAGTTCTGGTGTTGT+TGG 0.477113 3_1:-46825300 Msa0359370:CDS
GATGGTGGAGTAGGTTGGTT+AGG 0.483841 3_1:+46825393 None:intergenic
ACCAGAACTCCTTTCGTACT+CGG 0.488981 3_1:+46825308 None:intergenic
ACGTTTATGCGTAACAACTC+TGG 0.489769 3_1:-46825820 Msa0359370:CDS
TTAGGTGATGGAGGTTGGTA+CGG 0.489944 3_1:+46825411 None:intergenic
ACAAAGATGGCTATGTAGCT+TGG 0.511243 3_1:-46826047 Msa0359370:CDS
GTAGGTTGGTTAGGTGATGG+AGG 0.512588 3_1:+46825402 None:intergenic
TTGTTTACTAAGTGAACCAT+TGG 0.514833 3_1:+46825957 None:intergenic
CAACTCATTAGTTTCTTCCA+TGG 0.518120 3_1:+46826023 None:intergenic
TGTCATTTCTGTAGTAGTGC+AGG 0.521378 3_1:+46825261 None:intergenic
GAAATAGATCCGAGTACGAA+AGG 0.521953 3_1:-46825317 Msa0359370:CDS
AATGGGATGGTGGAGTAGGT+TGG 0.522124 3_1:+46825388 None:intergenic
GGAGTAGGTTGGTTAGGTGA+TGG 0.533493 3_1:+46825399 None:intergenic
TTGGTGGATGCTATAAATAG+AGG 0.536963 3_1:-46825991 Msa0359370:CDS
AAATAATAGAGGGATGGGTA+AGG 0.543328 3_1:-46826070 None:intergenic
TTGGAGGTGGAGTGTCTAGC+GGG 0.545573 3_1:-46825569 Msa0359370:CDS
ATTACTTGAGCCGAATGAAG+TGG 0.570458 3_1:-46825870 Msa0359370:CDS
TGGCTATGTAGCTTGGACCA+TGG 0.580657 3_1:-46826040 Msa0359370:CDS
GTTGTTGGGATCAACAATCA+AGG 0.582452 3_1:-46825285 Msa0359370:CDS
TATTTGAAGATTCTTGTTGG+AGG 0.595361 3_1:-46825585 Msa0359370:CDS
TGGTGGATGCTATAAATAGA+GGG 0.599601 3_1:-46825990 Msa0359370:CDS
TTCTATAGCATTAGACGCTG+AGG 0.600384 3_1:-46825541 Msa0359370:CDS
GGTGATGGAGGTTGGTACGG+TGG 0.605354 3_1:+46825414 None:intergenic
GTTGGAGGTGGAGTGTCTAG+CGG 0.610018 3_1:-46825570 Msa0359370:CDS
TTCAGTTGCAAAGACTTACA+CGG 0.635359 3_1:-46825785 Msa0359370:CDS
AGAGGGTTACGTGATGCCAA+TGG 0.638045 3_1:-46825973 Msa0359370:CDS
TATTTATAGCATCCACCAAG+AGG 0.660190 3_1:+46825995 None:intergenic
GGGATGGGTAAGGACAAAGA+TGG 0.668592 3_1:-46826060 Msa0359370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CGTTTTTTGTAATTCAGTTT+TGG + chr3_1:46825423-46825442 None:intergenic 25.0%
! AGTATGGAGTAATTACTTAA+AGG - chr3_1:46825568-46825587 Msa0359370:CDS 25.0%
! GATTATTTGAAGATTCTTGT+TGG - chr3_1:46825732-46825751 Msa0359370:intron 25.0%
! GTTATGAATGCATCATTATT+GGG + chr3_1:46825870-46825889 None:intergenic 25.0%
! TGTTATGAATGCATCATTAT+TGG + chr3_1:46825871-46825890 None:intergenic 25.0%
! CTCTGAATCTAAAGAAAAAT+GGG + chr3_1:46825952-46825971 None:intergenic 25.0%
!!! CGTTTTTTGTAATTCAGTTT+TGG + chr3_1:46825423-46825442 None:intergenic 25.0%
! AGTATGGAGTAATTACTTAA+AGG - chr3_1:46825568-46825587 Msa0359370:CDS 25.0%
! GATTATTTGAAGATTCTTGT+TGG - chr3_1:46825732-46825751 Msa0359370:intron 25.0%
! GTTATGAATGCATCATTATT+GGG + chr3_1:46825870-46825889 None:intergenic 25.0%
! TGTTATGAATGCATCATTAT+TGG + chr3_1:46825871-46825890 None:intergenic 25.0%
! CTCTGAATCTAAAGAAAAAT+GGG + chr3_1:46825952-46825971 None:intergenic 25.0%
TTGTTTACTAAGTGAACCAT+TGG + chr3_1:46825366-46825385 None:intergenic 30.0%
!! GAATATTTTTGTGAGATGGA+TGG + chr3_1:46825689-46825708 None:intergenic 30.0%
TATTTGAAGATTCTTGTTGG+AGG - chr3_1:46825735-46825754 Msa0359370:intron 30.0%
!!! AGAAAGTGAAAGTTTTGGAA+TGG - chr3_1:46825827-46825846 Msa0359370:CDS 30.0%
GAATCTAAAGAAAAATGGGA+TGG + chr3_1:46825948-46825967 None:intergenic 30.0%
CCTCTGAATCTAAAGAAAAA+TGG + chr3_1:46825953-46825972 None:intergenic 30.0%
!! CCATTTTTCTTTAGATTCAG+AGG - chr3_1:46825950-46825969 Msa0359370:CDS 30.0%
!! TGACGATTTCGTTTTTCAAT+AGG + chr3_1:46825978-46825997 None:intergenic 30.0%
TTGTTTACTAAGTGAACCAT+TGG + chr3_1:46825366-46825385 None:intergenic 30.0%
!! GAATATTTTTGTGAGATGGA+TGG + chr3_1:46825689-46825708 None:intergenic 30.0%
TATTTGAAGATTCTTGTTGG+AGG - chr3_1:46825735-46825754 Msa0359370:intron 30.0%
!!! AGAAAGTGAAAGTTTTGGAA+TGG - chr3_1:46825827-46825846 Msa0359370:CDS 30.0%
GAATCTAAAGAAAAATGGGA+TGG + chr3_1:46825948-46825967 None:intergenic 30.0%
CCTCTGAATCTAAAGAAAAA+TGG + chr3_1:46825953-46825972 None:intergenic 30.0%
!! CCATTTTTCTTTAGATTCAG+AGG - chr3_1:46825950-46825969 Msa0359370:CDS 30.0%
!! TGACGATTTCGTTTTTCAAT+AGG + chr3_1:46825978-46825997 None:intergenic 30.0%
! CAACTCATTAGTTTCTTCCA+TGG + chr3_1:46825300-46825319 None:intergenic 35.0%
! TAATGAGTTGTTGTTCCTCT+TGG - chr3_1:46825310-46825329 Msa0359370:CDS 35.0%
TATTTATAGCATCCACCAAG+AGG + chr3_1:46825328-46825347 None:intergenic 35.0%
! TTGGTGGATGCTATAAATAG+AGG - chr3_1:46825329-46825348 Msa0359370:CDS 35.0%
! TGGTGGATGCTATAAATAGA+GGG - chr3_1:46825330-46825349 Msa0359370:CDS 35.0%
!! GAGTTCTAAACCACTTCATT+CGG + chr3_1:46825463-46825482 None:intergenic 35.0%
!!! TAACAACTCTGGTTTTGGAT+GGG - chr3_1:46825511-46825530 Msa0359370:CDS 35.0%
TTCAGTTGCAAAGACTTACA+CGG - chr3_1:46825535-46825554 Msa0359370:CDS 35.0%
!! CCTCGAATATTTTTGTGAGA+TGG + chr3_1:46825693-46825712 None:intergenic 35.0%
CCATCTCACAAAAATATTCG+AGG - chr3_1:46825690-46825709 Msa0359370:intron 35.0%
! GAGCAAGAAAGTGAAAGTTT+TGG - chr3_1:46825822-46825841 Msa0359370:CDS 35.0%
!!! AGTGAAAGTTTTGGAATGGA+AGG - chr3_1:46825831-46825850 Msa0359370:CDS 35.0%
!!! GTGAAAGTTTTGGAATGGAA+GGG - chr3_1:46825832-46825851 Msa0359370:CDS 35.0%
TACGGTGGTTCATAATTATC+TGG + chr3_1:46825894-46825913 None:intergenic 35.0%
TCTAAAGAAAAATGGGATGG+TGG + chr3_1:46825945-46825964 None:intergenic 35.0%
! CAACTCATTAGTTTCTTCCA+TGG + chr3_1:46825300-46825319 None:intergenic 35.0%
! TAATGAGTTGTTGTTCCTCT+TGG - chr3_1:46825310-46825329 Msa0359370:CDS 35.0%
TATTTATAGCATCCACCAAG+AGG + chr3_1:46825328-46825347 None:intergenic 35.0%
! TTGGTGGATGCTATAAATAG+AGG - chr3_1:46825329-46825348 Msa0359370:CDS 35.0%
! TGGTGGATGCTATAAATAGA+GGG - chr3_1:46825330-46825349 Msa0359370:CDS 35.0%
!! GAGTTCTAAACCACTTCATT+CGG + chr3_1:46825463-46825482 None:intergenic 35.0%
!!! TAACAACTCTGGTTTTGGAT+GGG - chr3_1:46825511-46825530 Msa0359370:CDS 35.0%
TTCAGTTGCAAAGACTTACA+CGG - chr3_1:46825535-46825554 Msa0359370:CDS 35.0%
!! CCTCGAATATTTTTGTGAGA+TGG + chr3_1:46825693-46825712 None:intergenic 35.0%
CCATCTCACAAAAATATTCG+AGG - chr3_1:46825690-46825709 Msa0359370:intron 35.0%
! GAGCAAGAAAGTGAAAGTTT+TGG - chr3_1:46825822-46825841 Msa0359370:CDS 35.0%
!!! AGTGAAAGTTTTGGAATGGA+AGG - chr3_1:46825831-46825850 Msa0359370:CDS 35.0%
!!! GTGAAAGTTTTGGAATGGAA+GGG - chr3_1:46825832-46825851 Msa0359370:CDS 35.0%
TACGGTGGTTCATAATTATC+TGG + chr3_1:46825894-46825913 None:intergenic 35.0%
TCTAAAGAAAAATGGGATGG+TGG + chr3_1:46825945-46825964 None:intergenic 35.0%
ACAAAGATGGCTATGTAGCT+TGG - chr3_1:46825273-46825292 Msa0359370:CDS 40.0%
!!! TTTTGGAATTGAGCTGAGGA+AGG + chr3_1:46825406-46825425 None:intergenic 40.0%
!!! TCAGTTTTGGAATTGAGCTG+AGG + chr3_1:46825410-46825429 None:intergenic 40.0%
ATTACTTGAGCCGAATGAAG+TGG - chr3_1:46825450-46825469 Msa0359370:CDS 40.0%
ACGTTTATGCGTAACAACTC+TGG - chr3_1:46825500-46825519 Msa0359370:CDS 40.0%
!! ATGCGTAACAACTCTGGTTT+TGG - chr3_1:46825506-46825525 Msa0359370:CDS 40.0%
!!! GTAACAACTCTGGTTTTGGA+TGG - chr3_1:46825510-46825529 Msa0359370:CDS 40.0%
TTGAAGATTCTTGTTGGAGG+TGG - chr3_1:46825738-46825757 Msa0359370:intron 40.0%
TTCTATAGCATTAGACGCTG+AGG - chr3_1:46825779-46825798 Msa0359370:CDS 40.0%
GAAATAGATCCGAGTACGAA+AGG - chr3_1:46826003-46826022 Msa0359370:CDS 40.0%
!! AAAGGAGTTCTGGTGTTGTT+GGG - chr3_1:46826021-46826040 Msa0359370:CDS 40.0%
GTTGTTGGGATCAACAATCA+AGG - chr3_1:46826035-46826054 Msa0359370:CDS 40.0%
ACAAAGATGGCTATGTAGCT+TGG - chr3_1:46825273-46825292 Msa0359370:CDS 40.0%
!!! TTTTGGAATTGAGCTGAGGA+AGG + chr3_1:46825406-46825425 None:intergenic 40.0%
!!! TCAGTTTTGGAATTGAGCTG+AGG + chr3_1:46825410-46825429 None:intergenic 40.0%
ATTACTTGAGCCGAATGAAG+TGG - chr3_1:46825450-46825469 Msa0359370:CDS 40.0%
ACGTTTATGCGTAACAACTC+TGG - chr3_1:46825500-46825519 Msa0359370:CDS 40.0%
!! ATGCGTAACAACTCTGGTTT+TGG - chr3_1:46825506-46825525 Msa0359370:CDS 40.0%
!!! GTAACAACTCTGGTTTTGGA+TGG - chr3_1:46825510-46825529 Msa0359370:CDS 40.0%
TTGAAGATTCTTGTTGGAGG+TGG - chr3_1:46825738-46825757 Msa0359370:intron 40.0%
TTCTATAGCATTAGACGCTG+AGG - chr3_1:46825779-46825798 Msa0359370:CDS 40.0%
GAAATAGATCCGAGTACGAA+AGG - chr3_1:46826003-46826022 Msa0359370:CDS 40.0%
!! AAAGGAGTTCTGGTGTTGTT+GGG - chr3_1:46826021-46826040 Msa0359370:CDS 40.0%
GTTGTTGGGATCAACAATCA+AGG - chr3_1:46826035-46826054 Msa0359370:CDS 40.0%
! TGAGTTGTTGTTCCTCTTGG+TGG - chr3_1:46825313-46825332 Msa0359370:CDS 45.0%
ACACGGCTACTGAAGAAGTA+TGG - chr3_1:46825552-46825571 Msa0359370:CDS 45.0%
!! TTAGGTGATGGAGGTTGGTA+CGG + chr3_1:46825912-46825931 None:intergenic 45.0%
!! GAAAAATGGGATGGTGGAGT+AGG + chr3_1:46825939-46825958 None:intergenic 45.0%
ACCAGAACTCCTTTCGTACT+CGG + chr3_1:46826015-46826034 None:intergenic 45.0%
!! GAAAGGAGTTCTGGTGTTGT+TGG - chr3_1:46826020-46826039 Msa0359370:CDS 45.0%
! TGAGTTGTTGTTCCTCTTGG+TGG - chr3_1:46825313-46825332 Msa0359370:CDS 45.0%
ACACGGCTACTGAAGAAGTA+TGG - chr3_1:46825552-46825571 Msa0359370:CDS 45.0%
!! TTAGGTGATGGAGGTTGGTA+CGG + chr3_1:46825912-46825931 None:intergenic 45.0%
!! GAAAAATGGGATGGTGGAGT+AGG + chr3_1:46825939-46825958 None:intergenic 45.0%
ACCAGAACTCCTTTCGTACT+CGG + chr3_1:46826015-46826034 None:intergenic 45.0%
!! GAAAGGAGTTCTGGTGTTGT+TGG - chr3_1:46826020-46826039 Msa0359370:CDS 45.0%
TGGCTATGTAGCTTGGACCA+TGG - chr3_1:46825280-46825299 Msa0359370:CDS 50.0%
AGAGGGTTACGTGATGCCAA+TGG - chr3_1:46825347-46825366 Msa0359370:CDS 50.0%
!! GTTGGTTAGGTGATGGAGGT+TGG + chr3_1:46825917-46825936 None:intergenic 50.0%
!! GTAGGTTGGTTAGGTGATGG+AGG + chr3_1:46825921-46825940 None:intergenic 50.0%
!! GGAGTAGGTTGGTTAGGTGA+TGG + chr3_1:46825924-46825943 None:intergenic 50.0%
!! GATGGTGGAGTAGGTTGGTT+AGG + chr3_1:46825930-46825949 None:intergenic 50.0%
!! AATGGGATGGTGGAGTAGGT+TGG + chr3_1:46825935-46825954 None:intergenic 50.0%
TCCGAGTACGAAAGGAGTTC+TGG - chr3_1:46826011-46826030 Msa0359370:CDS 50.0%
TGGCTATGTAGCTTGGACCA+TGG - chr3_1:46825280-46825299 Msa0359370:CDS 50.0%
AGAGGGTTACGTGATGCCAA+TGG - chr3_1:46825347-46825366 Msa0359370:CDS 50.0%
!! GTTGGTTAGGTGATGGAGGT+TGG + chr3_1:46825917-46825936 None:intergenic 50.0%
!! GTAGGTTGGTTAGGTGATGG+AGG + chr3_1:46825921-46825940 None:intergenic 50.0%
!! GGAGTAGGTTGGTTAGGTGA+TGG + chr3_1:46825924-46825943 None:intergenic 50.0%
!! GATGGTGGAGTAGGTTGGTT+AGG + chr3_1:46825930-46825949 None:intergenic 50.0%
!! AATGGGATGGTGGAGTAGGT+TGG + chr3_1:46825935-46825954 None:intergenic 50.0%
TCCGAGTACGAAAGGAGTTC+TGG - chr3_1:46826011-46826030 Msa0359370:CDS 50.0%
! GTTGGAGGTGGAGTGTCTAG+CGG - chr3_1:46825750-46825769 Msa0359370:intron 55.0%
! TTGGAGGTGGAGTGTCTAGC+GGG - chr3_1:46825751-46825770 Msa0359370:intron 55.0%
! GTTGGAGGTGGAGTGTCTAG+CGG - chr3_1:46825750-46825769 Msa0359370:intron 55.0%
! TTGGAGGTGGAGTGTCTAGC+GGG - chr3_1:46825751-46825770 Msa0359370:intron 55.0%
!! GGTGATGGAGGTTGGTACGG+TGG + chr3_1:46825909-46825928 None:intergenic 60.0%
!! GGTGATGGAGGTTGGTACGG+TGG + chr3_1:46825909-46825928 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 46825263 46826079 46825263 ID=Msa0359370;Name=Msa0359370
chr3_1 mRNA 46825263 46826079 46825263 ID=Msa0359370-mRNA-1;Parent=Msa0359370;Name=Msa0359370-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|2|0|240
chr3_1 exon 46825753 46826079 46825753 ID=Msa0359370-mRNA-1:exon:8266;Parent=Msa0359370-mRNA-1
chr3_1 exon 46825263 46825658 46825263 ID=Msa0359370-mRNA-1:exon:8265;Parent=Msa0359370-mRNA-1
chr3_1 CDS 46825753 46826079 46825753 ID=Msa0359370-mRNA-1:cds;Parent=Msa0359370-mRNA-1
chr3_1 CDS 46825263 46825658 46825263 ID=Msa0359370-mRNA-1:cds;Parent=Msa0359370-mRNA-1
Gene Sequence

>Msa0359370

ATGGGTAAGGACAAAGATGGCTATGTAGCTTGGACCATGGAAGAAACTAATGAGTTGTTGTTCCTCTTGGTGGATGCTATAAATAGAGGGTTACGTGATGCCAATGGTTCACTTAGTAAACAAACTGTAGAGCGAGTTATCCTTCCTCAGCTCAATTCCAAAACTGAATTACAAAAAACGTATAATCATTACTTGAGCCGAATGAAGTGGTTTAGAACTCAATACAACAAGATGACAACGTTTATGCGTAACAACTCTGGTTTTGGATGGGATTCAGTTGCAAAGACTTACACGGCTACTGAAGAAGTATGGAGTAATTACTTAAAGTGCCATCCATCTCACAAAAATATTCGAGGAAAAAGTATGATTGATTATGATTATTTGAAGATTCTTGTTGGAGGTGGAGTGTCTAGCGGGAATAGTTCTATAGCATTAGACGCTGAGGATACTGATGCAACAACTTTTGAGCAAGAAAGTGAAAGTTTTGGAATGGAAGGGTTTTCATATGATCCCAATAATGATGCATTCATAACACCAGATAATTATGAACCACCGTACCAACCTCCATCACCTAACCAACCTACTCCACCATCCCATTTTTCTTTAGATTCAGAGGCTCCTATTGAAAAACGAAATCGTCATAAGAGAAATAGATCCGAGTACGAAAGGAGTTCTGGTGTTGTTGGGATCAACAATCAAGGACCTGCACTACTACAGAAATGA

Protein sequence

>Msa0359370

MGKDKDGYVAWTMEETNELLFLLVDAINRGLRDANGSLSKQTVERVILPQLNSKTELQKTYNHYLSRMKWFRTQYNKMTTFMRNNSGFGWDSVAKTYTATEEVWSNYLKCHPSHKNIRGKSMIDYDYLKILVGGGVSSGNSSIALDAEDTDATTFEQESESFGMEGFSYDPNNDAFITPDNYEPPYQPPSPNQPTPPSHFSLDSEAPIEKRNRHKRNRSEYERSSGVVGINNQGPALLQK*