AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0787600


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787600 MtrunA17_Chr1g0207381 88.034 234 21 2 1 227 1 234 1.96e-152 427
Msa0787600 MtrunA17_Chr4g0051721 89.637 193 20 0 1 193 1 193 9.50e-131 366
Msa0787600 MtrunA17_Chr3g0093841 29.193 161 110 3 9 169 15 171 1.52e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0787600 AT5G27260.1 43.976 166 87 2 1 166 1 160 9.95e-42 144
Msa0787600 AT2G29880.1 41.520 171 99 1 1 171 1 170 6.38e-41 142
Msa0787600 AT1G30140.2 35.862 145 89 1 12 156 7 147 5.67e-30 111
Msa0787600 AT1G30140.1 35.862 145 89 1 12 156 7 147 5.67e-30 111

Find 39 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCATAACAACTCTGGTTT+TGG 0.268759 5_2:+73514022 Msa0787600:CDS
TGAGTTGTTGTTCCTCTTAG+TGG 0.299201 5_2:+73513829 Msa0787600:CDS
TACGGTGGTTCATAGTTATC+TGG 0.342012 5_2:-73514407 None:intergenic
GAGCAAGAAAGTGAAAGTTT+TGG 0.364343 5_2:+73514338 Msa0787600:CDS
AGTATGGAGTAATTACTTAA+AGG 0.368835 5_2:+73514084 Msa0787600:CDS
GAGTTCTAAACCACTTCATT+CGG 0.405087 5_2:-73513976 None:intergenic
GATTATTTGAAGATTCTTGT+TGG 0.439501 5_2:+73514248 Msa0787600:CDS
TGCTATGAATGCATCACTCT+TGG 0.440181 5_2:-73514384 None:intergenic
TTGAAGATTCTTGTTGGAGG+TGG 0.445118 5_2:+73514254 Msa0787600:CDS
GTTGGTTAGGTGATGGAGGT+TGG 0.445510 5_2:-73514430 None:intergenic
ACACGGCTACTGAAGAAGTA+TGG 0.447236 5_2:+73514068 Msa0787600:CDS
TCACAACAAGAAAATAATAG+AGG 0.460305 5_2:+73513755 Msa0787600:CDS
TTAGTGGATGCTATAAATAG+AGG 0.470322 5_2:+73513845 Msa0787600:CDS
GCTATGAATGCATCACTCTT+GGG 0.472761 5_2:-73514383 None:intergenic
GATGGTGGAGTAGGTTGGTT+AGG 0.483841 5_2:-73514443 None:intergenic
TTAGGTGATGGAGGTTGGTA+CGG 0.489944 5_2:-73514425 None:intergenic
ACAAAGATGGCTATGTAGCT+TGG 0.511243 5_2:+73513789 Msa0787600:CDS
GTAGGTTGGTTAGGTGATGG+AGG 0.512588 5_2:-73514434 None:intergenic
ACGTTTATGCATAACAACTC+TGG 0.513410 5_2:+73514016 Msa0787600:CDS
CAACTCATTAGTTTCTTCCA+TGG 0.518120 5_2:-73513813 None:intergenic
GAGGGTTACGTGATGCCAAT+GGG 0.518455 5_2:+73513864 Msa0787600:CDS
TATTTATAGCATCCACTAAG+AGG 0.521289 5_2:-73513841 None:intergenic
AATGGGATGGTGGAGTAGGT+TGG 0.522124 5_2:-73514448 None:intergenic
CAAGAAAATAATAGAGGGAA+AGG 0.525413 5_2:+73513761 Msa0787600:CDS
GGAGTAGGTTGGTTAGGTGA+TGG 0.533493 5_2:-73514437 None:intergenic
TTGTTTACTAAGTGACCCAT+TGG 0.535974 5_2:-73513879 None:intergenic
TTGGAGGTGGAGTGTCTAGC+GGG 0.545573 5_2:+73514267 Msa0787600:CDS
AGAGGGTTACGTGATGCCAA+TGG 0.546890 5_2:+73513863 Msa0787600:CDS
AAATAATAGAGGGAAAGGTA+AGG 0.567458 5_2:+73513766 Msa0787600:CDS
ATTACTTGAGCCGAATGAAG+TGG 0.570458 5_2:+73513966 Msa0787600:CDS
TGGCTATGTAGCTTGGACCA+TGG 0.580657 5_2:+73513796 Msa0787600:CDS
GGGAAAGGTAAGGACAAAGA+TGG 0.591698 5_2:+73513776 Msa0787600:CDS
CACAACAAGAAAATAATAGA+GGG 0.595053 5_2:+73513756 Msa0787600:CDS
TATTTGAAGATTCTTGTTGG+AGG 0.595361 5_2:+73514251 Msa0787600:CDS
TTCTATAGCATTAGACGCTG+AGG 0.600384 5_2:+73514295 Msa0787600:CDS
TAGTGGATGCTATAAATAGA+GGG 0.602040 5_2:+73513846 Msa0787600:CDS
GGTGATGGAGGTTGGTACGG+TGG 0.605354 5_2:-73514422 None:intergenic
GTTGGAGGTGGAGTGTCTAG+CGG 0.610018 5_2:+73514266 Msa0787600:CDS
TTCAGTTGCAAAGACTTACA+CGG 0.635359 5_2:+73514051 Msa0787600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TCACAACAAGAAAATAATAG+AGG + chr5_2:73513755-73513774 Msa0787600:CDS 25.0%
! CACAACAAGAAAATAATAGA+GGG + chr5_2:73513756-73513775 Msa0787600:CDS 25.0%
!!! AGTTTTTTGTGATTCAGTTT+TGG - chr5_2:73513939-73513958 None:intergenic 25.0%
! AGTATGGAGTAATTACTTAA+AGG + chr5_2:73514084-73514103 Msa0787600:CDS 25.0%
!!! AATATTTTTGTGAGATGGAT+GGG - chr5_2:73514204-73514223 None:intergenic 25.0%
! GATTATTTGAAGATTCTTGT+TGG + chr5_2:73514248-73514267 Msa0787600:CDS 25.0%
! CTCTGAATCTAAAGAAAAAT+GGG - chr5_2:73514468-73514487 None:intergenic 25.0%
! TCACAACAAGAAAATAATAG+AGG + chr5_2:73513755-73513774 Msa0787600:CDS 25.0%
! CACAACAAGAAAATAATAGA+GGG + chr5_2:73513756-73513775 Msa0787600:CDS 25.0%
!!! AGTTTTTTGTGATTCAGTTT+TGG - chr5_2:73513939-73513958 None:intergenic 25.0%
! AGTATGGAGTAATTACTTAA+AGG + chr5_2:73514084-73514103 Msa0787600:CDS 25.0%
!!! AATATTTTTGTGAGATGGAT+GGG - chr5_2:73514204-73514223 None:intergenic 25.0%
! GATTATTTGAAGATTCTTGT+TGG + chr5_2:73514248-73514267 Msa0787600:CDS 25.0%
! CTCTGAATCTAAAGAAAAAT+GGG - chr5_2:73514468-73514487 None:intergenic 25.0%
CAAGAAAATAATAGAGGGAA+AGG + chr5_2:73513761-73513780 Msa0787600:CDS 30.0%
AAATAATAGAGGGAAAGGTA+AGG + chr5_2:73513766-73513785 Msa0787600:CDS 30.0%
TATTTATAGCATCCACTAAG+AGG - chr5_2:73513844-73513863 None:intergenic 30.0%
! TTAGTGGATGCTATAAATAG+AGG + chr5_2:73513845-73513864 Msa0787600:CDS 30.0%
! TAGTGGATGCTATAAATAGA+GGG + chr5_2:73513846-73513865 Msa0787600:CDS 30.0%
!! GAATATTTTTGTGAGATGGA+TGG - chr5_2:73514205-73514224 None:intergenic 30.0%
TATTTGAAGATTCTTGTTGG+AGG + chr5_2:73514251-73514270 Msa0787600:CDS 30.0%
!!! AGAAAGTGAAAGTTTTGGAA+TGG + chr5_2:73514343-73514362 Msa0787600:CDS 30.0%
GAATCTAAAGAAAAATGGGA+TGG - chr5_2:73514464-73514483 None:intergenic 30.0%
CCTCTGAATCTAAAGAAAAA+TGG - chr5_2:73514469-73514488 None:intergenic 30.0%
!! CCATTTTTCTTTAGATTCAG+AGG + chr5_2:73514466-73514485 Msa0787600:CDS 30.0%
!! TGACGATTTCGTTTTTCAAT+AGG - chr5_2:73514494-73514513 None:intergenic 30.0%
CAAGAAAATAATAGAGGGAA+AGG + chr5_2:73513761-73513780 Msa0787600:CDS 30.0%
AAATAATAGAGGGAAAGGTA+AGG + chr5_2:73513766-73513785 Msa0787600:CDS 30.0%
TATTTATAGCATCCACTAAG+AGG - chr5_2:73513844-73513863 None:intergenic 30.0%
! TTAGTGGATGCTATAAATAG+AGG + chr5_2:73513845-73513864 Msa0787600:CDS 30.0%
! TAGTGGATGCTATAAATAGA+GGG + chr5_2:73513846-73513865 Msa0787600:CDS 30.0%
!! GAATATTTTTGTGAGATGGA+TGG - chr5_2:73514205-73514224 None:intergenic 30.0%
TATTTGAAGATTCTTGTTGG+AGG + chr5_2:73514251-73514270 Msa0787600:CDS 30.0%
!!! AGAAAGTGAAAGTTTTGGAA+TGG + chr5_2:73514343-73514362 Msa0787600:CDS 30.0%
GAATCTAAAGAAAAATGGGA+TGG - chr5_2:73514464-73514483 None:intergenic 30.0%
CCTCTGAATCTAAAGAAAAA+TGG - chr5_2:73514469-73514488 None:intergenic 30.0%
!! CCATTTTTCTTTAGATTCAG+AGG + chr5_2:73514466-73514485 Msa0787600:CDS 30.0%
!! TGACGATTTCGTTTTTCAAT+AGG - chr5_2:73514494-73514513 None:intergenic 30.0%
! CAACTCATTAGTTTCTTCCA+TGG - chr5_2:73513816-73513835 None:intergenic 35.0%
TTGTTTACTAAGTGACCCAT+TGG - chr5_2:73513882-73513901 None:intergenic 35.0%
!! GAGTTCTAAACCACTTCATT+CGG - chr5_2:73513979-73513998 None:intergenic 35.0%
ACGTTTATGCATAACAACTC+TGG + chr5_2:73514016-73514035 Msa0787600:CDS 35.0%
!! ATGCATAACAACTCTGGTTT+TGG + chr5_2:73514022-73514041 Msa0787600:CDS 35.0%
!!! ATAACAACTCTGGTTTTGGA+TGG + chr5_2:73514026-73514045 Msa0787600:CDS 35.0%
!!! TAACAACTCTGGTTTTGGAT+GGG + chr5_2:73514027-73514046 Msa0787600:CDS 35.0%
TTCAGTTGCAAAGACTTACA+CGG + chr5_2:73514051-73514070 Msa0787600:CDS 35.0%
!! CCTCGAATATTTTTGTGAGA+TGG - chr5_2:73514209-73514228 None:intergenic 35.0%
CCATCTCACAAAAATATTCG+AGG + chr5_2:73514206-73514225 Msa0787600:CDS 35.0%
! GAGCAAGAAAGTGAAAGTTT+TGG + chr5_2:73514338-73514357 Msa0787600:CDS 35.0%
!!! AGTGAAAGTTTTGGAATGGA+AGG + chr5_2:73514347-73514366 Msa0787600:CDS 35.0%
!!! GTGAAAGTTTTGGAATGGAA+GGG + chr5_2:73514348-73514367 Msa0787600:CDS 35.0%
TCTAAAGAAAAATGGGATGG+TGG - chr5_2:73514461-73514480 None:intergenic 35.0%
! CAACTCATTAGTTTCTTCCA+TGG - chr5_2:73513816-73513835 None:intergenic 35.0%
TTGTTTACTAAGTGACCCAT+TGG - chr5_2:73513882-73513901 None:intergenic 35.0%
!! GAGTTCTAAACCACTTCATT+CGG - chr5_2:73513979-73513998 None:intergenic 35.0%
ACGTTTATGCATAACAACTC+TGG + chr5_2:73514016-73514035 Msa0787600:CDS 35.0%
!! ATGCATAACAACTCTGGTTT+TGG + chr5_2:73514022-73514041 Msa0787600:CDS 35.0%
!!! ATAACAACTCTGGTTTTGGA+TGG + chr5_2:73514026-73514045 Msa0787600:CDS 35.0%
!!! TAACAACTCTGGTTTTGGAT+GGG + chr5_2:73514027-73514046 Msa0787600:CDS 35.0%
TTCAGTTGCAAAGACTTACA+CGG + chr5_2:73514051-73514070 Msa0787600:CDS 35.0%
!! CCTCGAATATTTTTGTGAGA+TGG - chr5_2:73514209-73514228 None:intergenic 35.0%
CCATCTCACAAAAATATTCG+AGG + chr5_2:73514206-73514225 Msa0787600:CDS 35.0%
! GAGCAAGAAAGTGAAAGTTT+TGG + chr5_2:73514338-73514357 Msa0787600:CDS 35.0%
!!! AGTGAAAGTTTTGGAATGGA+AGG + chr5_2:73514347-73514366 Msa0787600:CDS 35.0%
!!! GTGAAAGTTTTGGAATGGAA+GGG + chr5_2:73514348-73514367 Msa0787600:CDS 35.0%
TCTAAAGAAAAATGGGATGG+TGG - chr5_2:73514461-73514480 None:intergenic 35.0%
ACAAAGATGGCTATGTAGCT+TGG + chr5_2:73513789-73513808 Msa0787600:CDS 40.0%
! TGAGTTGTTGTTCCTCTTAG+TGG + chr5_2:73513829-73513848 Msa0787600:CDS 40.0%
!! TTTTGGTATTGAGCTGAGGA+AGG - chr5_2:73513922-73513941 None:intergenic 40.0%
!! TCAGTTTTGGTATTGAGCTG+AGG - chr5_2:73513926-73513945 None:intergenic 40.0%
ATTACTTGAGCCGAATGAAG+TGG + chr5_2:73513966-73513985 Msa0787600:CDS 40.0%
TTGAAGATTCTTGTTGGAGG+TGG + chr5_2:73514254-73514273 Msa0787600:CDS 40.0%
TTCTATAGCATTAGACGCTG+AGG + chr5_2:73514295-73514314 Msa0787600:CDS 40.0%
GCTATGAATGCATCACTCTT+GGG - chr5_2:73514386-73514405 None:intergenic 40.0%
TGCTATGAATGCATCACTCT+TGG - chr5_2:73514387-73514406 None:intergenic 40.0%
TACGGTGGTTCATAGTTATC+TGG - chr5_2:73514410-73514429 None:intergenic 40.0%
ACAAAGATGGCTATGTAGCT+TGG + chr5_2:73513789-73513808 Msa0787600:CDS 40.0%
! TGAGTTGTTGTTCCTCTTAG+TGG + chr5_2:73513829-73513848 Msa0787600:CDS 40.0%
!! TTTTGGTATTGAGCTGAGGA+AGG - chr5_2:73513922-73513941 None:intergenic 40.0%
!! TCAGTTTTGGTATTGAGCTG+AGG - chr5_2:73513926-73513945 None:intergenic 40.0%
ATTACTTGAGCCGAATGAAG+TGG + chr5_2:73513966-73513985 Msa0787600:CDS 40.0%
TTGAAGATTCTTGTTGGAGG+TGG + chr5_2:73514254-73514273 Msa0787600:CDS 40.0%
TTCTATAGCATTAGACGCTG+AGG + chr5_2:73514295-73514314 Msa0787600:CDS 40.0%
GCTATGAATGCATCACTCTT+GGG - chr5_2:73514386-73514405 None:intergenic 40.0%
TGCTATGAATGCATCACTCT+TGG - chr5_2:73514387-73514406 None:intergenic 40.0%
TACGGTGGTTCATAGTTATC+TGG - chr5_2:73514410-73514429 None:intergenic 40.0%
GGGAAAGGTAAGGACAAAGA+TGG + chr5_2:73513776-73513795 Msa0787600:CDS 45.0%
ACACGGCTACTGAAGAAGTA+TGG + chr5_2:73514068-73514087 Msa0787600:CDS 45.0%
!! TTAGGTGATGGAGGTTGGTA+CGG - chr5_2:73514428-73514447 None:intergenic 45.0%
!! GAAAAATGGGATGGTGGAGT+AGG - chr5_2:73514455-73514474 None:intergenic 45.0%
GGGAAAGGTAAGGACAAAGA+TGG + chr5_2:73513776-73513795 Msa0787600:CDS 45.0%
ACACGGCTACTGAAGAAGTA+TGG + chr5_2:73514068-73514087 Msa0787600:CDS 45.0%
!! TTAGGTGATGGAGGTTGGTA+CGG - chr5_2:73514428-73514447 None:intergenic 45.0%
!! GAAAAATGGGATGGTGGAGT+AGG - chr5_2:73514455-73514474 None:intergenic 45.0%
TGGCTATGTAGCTTGGACCA+TGG + chr5_2:73513796-73513815 Msa0787600:CDS 50.0%
AGAGGGTTACGTGATGCCAA+TGG + chr5_2:73513863-73513882 Msa0787600:CDS 50.0%
GAGGGTTACGTGATGCCAAT+GGG + chr5_2:73513864-73513883 Msa0787600:CDS 50.0%
!! GTTGGTTAGGTGATGGAGGT+TGG - chr5_2:73514433-73514452 None:intergenic 50.0%
!! GTAGGTTGGTTAGGTGATGG+AGG - chr5_2:73514437-73514456 None:intergenic 50.0%
!! GGAGTAGGTTGGTTAGGTGA+TGG - chr5_2:73514440-73514459 None:intergenic 50.0%
!! GATGGTGGAGTAGGTTGGTT+AGG - chr5_2:73514446-73514465 None:intergenic 50.0%
!! AATGGGATGGTGGAGTAGGT+TGG - chr5_2:73514451-73514470 None:intergenic 50.0%
TGGCTATGTAGCTTGGACCA+TGG + chr5_2:73513796-73513815 Msa0787600:CDS 50.0%
AGAGGGTTACGTGATGCCAA+TGG + chr5_2:73513863-73513882 Msa0787600:CDS 50.0%
GAGGGTTACGTGATGCCAAT+GGG + chr5_2:73513864-73513883 Msa0787600:CDS 50.0%
!! GTTGGTTAGGTGATGGAGGT+TGG - chr5_2:73514433-73514452 None:intergenic 50.0%
!! GTAGGTTGGTTAGGTGATGG+AGG - chr5_2:73514437-73514456 None:intergenic 50.0%
!! GGAGTAGGTTGGTTAGGTGA+TGG - chr5_2:73514440-73514459 None:intergenic 50.0%
!! GATGGTGGAGTAGGTTGGTT+AGG - chr5_2:73514446-73514465 None:intergenic 50.0%
!! AATGGGATGGTGGAGTAGGT+TGG - chr5_2:73514451-73514470 None:intergenic 50.0%
! GTTGGAGGTGGAGTGTCTAG+CGG + chr5_2:73514266-73514285 Msa0787600:CDS 55.0%
! TTGGAGGTGGAGTGTCTAGC+GGG + chr5_2:73514267-73514286 Msa0787600:CDS 55.0%
! GTTGGAGGTGGAGTGTCTAG+CGG + chr5_2:73514266-73514285 Msa0787600:CDS 55.0%
! TTGGAGGTGGAGTGTCTAGC+GGG + chr5_2:73514267-73514286 Msa0787600:CDS 55.0%
!! GGTGATGGAGGTTGGTACGG+TGG - chr5_2:73514425-73514444 None:intergenic 60.0%
!! GGTGATGGAGGTTGGTACGG+TGG - chr5_2:73514425-73514444 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 73513746 73514523 73513746 ID=Msa0787600;Name=Msa0787600
chr5_2 mRNA 73513746 73514523 73513746 ID=Msa0787600-mRNA-1;Parent=Msa0787600;Name=Msa0787600-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0|0|0|1|1|1|2|0|227
chr5_2 exon 73513746 73514105 73513746 ID=Msa0787600-mRNA-1:exon:22395;Parent=Msa0787600-mRNA-1
chr5_2 exon 73514200 73514523 73514200 ID=Msa0787600-mRNA-1:exon:22396;Parent=Msa0787600-mRNA-1
chr5_2 CDS 73513746 73514105 73513746 ID=Msa0787600-mRNA-1:cds;Parent=Msa0787600-mRNA-1
chr5_2 CDS 73514200 73514523 73514200 ID=Msa0787600-mRNA-1:cds;Parent=Msa0787600-mRNA-1
Gene Sequence

>Msa0787600

ATGGGGGATTCACAACAAGAAAATAATAGAGGGAAAGGTAAGGACAAAGATGGCTATGTAGCTTGGACCATGGAAGAAACTAATGAGTTGTTGTTCCTCTTAGTGGATGCTATAAATAGAGGGTTACGTGATGCCAATGGGTCACTTAGTAAACAAACTGTAGAGCGAGTTATCCTTCCTCAGCTCAATACCAAAACTGAATCACAAAAAACTTATAATCATTACTTGAGCCGAATGAAGTGGTTTAGAACTCAATACAACAAGATGACAACGTTTATGCATAACAACTCTGGTTTTGGATGGGATTCAGTTGCAAAGACTTACACGGCTACTGAAGAAGTATGGAGTAATTACTTAAAGTCCCATCCATCTCACAAAAATATTCGAGGAAAAAGTATGATTGATTATGATTATTTGAAGATTCTTGTTGGAGGTGGAGTGTCTAGCGGGAATAGTTCTATAGCATTAGACGCTGAGGATACTGATGCGACAACTTTTGAGCAAGAAAGTGAAAGTTTTGGAATGGAAGGGTTTTCATATGATCCCAAGAGTGATGCATTCATAGCACCAGATAACTATGAACCACCGTACCAACCTCCATCACCTAACCAACCTACTCCACCATCCCATTTTTCTTTAGATTCAGAGGCTCCTATTGAAAAACGAAATCGTCATAAGAGATAG

Protein sequence

>Msa0787600

MGDSQQENNRGKGKDKDGYVAWTMEETNELLFLLVDAINRGLRDANGSLSKQTVERVILPQLNTKTESQKTYNHYLSRMKWFRTQYNKMTTFMHNNSGFGWDSVAKTYTATEEVWSNYLKSHPSHKNIRGKSMIDYDYLKILVGGGVSSGNSSIALDAEDTDATTFEQESESFGMEGFSYDPKSDAFIAPDNYEPPYQPPSPNQPTPPSHFSLDSEAPIEKRNRHKR*