AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0376860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376860 MtrunA17_Chr3g0127451 98.507 134 2 0 1 134 1 134 2.42e-97 275
Msa0376860 MtrunA17_Chr4g0010061 65.909 132 45 0 1 132 14 145 1.78e-63 190
Msa0376860 MtrunA17_Chr8g0346311 58.088 136 54 1 1 133 1 136 1.67e-55 170
Msa0376860 MtrunA17_Chr7g0276081 56.296 135 57 1 2 134 3 137 1.23e-44 142
Msa0376860 MtrunA17_Chr5g0419651 64.103 78 28 0 7 84 7 84 2.30e-34 116
Msa0376860 MtrunA17_Chr3g0134591 34.058 138 82 1 3 131 2 139 1.40e-25 94.4
Msa0376860 MtrunA17_Chr3g0115151 42.500 80 45 1 1 80 457 535 4.67e-18 79.3
Msa0376860 MtrunA17_Chr3g0115131 45.333 75 40 1 6 80 517 590 3.73e-17 76.6
Msa0376860 MtrunA17_Chr5g0424491 40.000 80 47 1 1 80 513 591 2.28e-16 74.3
Msa0376860 MtrunA17_Chr6g0486891 42.857 70 39 1 12 81 50 118 7.88e-16 68.6
Msa0376860 MtrunA17_Chr4g0019781 34.568 81 53 0 3 83 137 217 2.72e-13 63.9
Msa0376860 MtrunA17_Chr8g0361931 38.571 70 42 1 9 78 10 78 3.81e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0376860 AT5G53560.1 79.104 134 28 0 1 134 1 134 2.75e-78 228
Msa0376860 AT2G32720.1 65.909 132 45 0 1 132 1 132 3.44e-66 197
Msa0376860 AT5G48810.1 59.559 136 53 1 1 134 1 136 3.40e-60 182
Msa0376860 AT2G32720.2 69.369 111 34 0 22 132 22 132 1.85e-54 167
Msa0376860 AT2G46650.1 51.163 129 61 1 7 133 4 132 1.38e-37 124
Msa0376860 AT1G26340.1 43.307 127 62 2 6 127 6 127 2.15e-35 119
Msa0376860 AT1G37130.1 42.500 80 45 1 1 80 538 616 7.42e-19 82.0
Msa0376860 AT1G60660.1 43.421 76 42 1 5 80 46 120 1.40e-17 73.6
Msa0376860 AT1G77760.1 40.000 80 47 1 1 80 541 619 4.34e-17 76.6
Msa0376860 AT3G61580.1 38.158 76 46 1 3 78 5 79 9.49e-13 64.3
Msa0376860 AT2G46210.1 38.571 70 42 1 9 78 11 79 4.09e-11 59.7
Msa0376860 AT5G09680.3 32.530 83 56 0 3 85 170 252 5.47e-11 58.5

Find 62 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCACAATCCAACATTATT+AGG 0.187332 3_1:-77184121 None:intergenic
TCAAATCTCTTCTTCACTTT+GGG 0.187989 3_1:-77181500 None:intergenic
CCGGGATGATCCTCCATAAA+AGG 0.201719 3_1:-77183560 None:intergenic
CCCCATAAACAGGAAAATTA+AGG 0.220364 3_1:-77184250 None:intergenic
CCTGGTGCCTCTCCTGATAT+TGG 0.232035 3_1:+77183888 Msa0376860:CDS
GTGGAACGTAGGTGCGCTTT+AGG 0.261273 3_1:-77183804 None:intergenic
GACAGTGTTTAGTTTCTTCT+TGG 0.292754 3_1:+77184021 Msa0376860:three_prime_UTR
CTGGTGCCTCTCCTGATATT+GGG 0.297310 3_1:+77183889 Msa0376860:CDS
AAGAAGGAACTACTCTTTCT+TGG 0.318516 3_1:-77183938 None:intergenic
GAGAATAAGCCAGCAATCTT+TGG 0.319930 3_1:-77181891 None:intergenic
GTTTAGCATCTTTGTTTCAT+TGG 0.320261 3_1:+77184157 Msa0376860:three_prime_UTR
GTCAAATCTCTTCTTCACTT+TGG 0.341887 3_1:-77181501 None:intergenic
TGGAACGTAGGTGCGCTTTA+GGG 0.342070 3_1:-77183803 None:intergenic
CACTTTGCATCCCCATAAAC+AGG 0.379042 3_1:-77184260 None:intergenic
GATTGCTGGCTTATTCTCTC+TGG 0.386144 3_1:+77181896 Msa0376860:CDS
CAACTTTCTGGTAATGGTAC+TGG 0.390866 3_1:+77184079 Msa0376860:three_prime_UTR
TCCCACATCTTCAAAATCAT+TGG 0.392408 3_1:-77183713 None:intergenic
GGTGTCTTGAGCATTGAAAC+TGG 0.406699 3_1:+77184042 Msa0376860:three_prime_UTR
TCATCGAAAGTGTGAAGCTT+TGG 0.412470 3_1:-77181848 None:intergenic
CAAAGGCTAAGCCCAATATC+AGG 0.426178 3_1:-77183900 None:intergenic
GGTCTGTTAGCTATTTATAG+AGG 0.431918 3_1:+77183987 Msa0376860:three_prime_UTR
CCCATAAACAGGAAAATTAA+GGG 0.432212 3_1:-77184249 None:intergenic
TCTCTTCTTCACTTTGGGGT+TGG 0.435970 3_1:-77181495 None:intergenic
CAAATCTCTTCTTCACTTTG+GGG 0.437199 3_1:-77181499 None:intergenic
AATTACCACTTCCGGTCCTT+GGG 0.440955 3_1:-77181555 None:intergenic
GAATTACCACTTCCGGTCCT+TGG 0.443825 3_1:-77181556 None:intergenic
CGGGATGATCCTCCATAAAA+GGG 0.446544 3_1:-77183559 None:intergenic
GAGGTTCTGTTGTCTGCAAC+AGG 0.466883 3_1:+77183590 Msa0376860:CDS
ACAACAAGACCAAAGATTGC+TGG 0.472357 3_1:+77181882 Msa0376860:CDS
CGCTTTAGGGGAACAGTGGA+TGG 0.473517 3_1:-77183790 None:intergenic
ACAAATTCCGAAGTCTTGTC+CGG 0.476643 3_1:-77183847 None:intergenic
CAAGAGGGAATTACCACTTC+CGG 0.485709 3_1:-77181563 None:intergenic
ATCATATTGCACCCTTGTGA+GGG 0.492456 3_1:+77183965 Msa0376860:three_prime_UTR
TGATTCTGCTAGAGAAATGA+TGG 0.506307 3_1:+77183741 Msa0376860:CDS
CAACAGAACCTCATCACCTC+CGG 0.513023 3_1:-77183579 None:intergenic
TGCAACTAGATTATTGTCCC+AGG 0.541641 3_1:+77184288 Msa0376860:three_prime_UTR
CTGGCTTATTCTCTCTGGAA+AGG 0.542076 3_1:+77181901 Msa0376860:CDS
CATCATATTGCACCCTTGTG+AGG 0.542934 3_1:+77183964 Msa0376860:three_prime_UTR
AATCTCCCAAGGACCGGAAG+TGG 0.548565 3_1:+77181550 Msa0376860:five_prime_UTR
CCAATATCAGGAGAGGCACC+AGG 0.552438 3_1:-77183888 None:intergenic
GTGCAATATGATGATAAAGA+AGG 0.560027 3_1:-77183954 None:intergenic
ATTTATAGAGGGTCATTCAA+AGG 0.568496 3_1:+77183999 Msa0376860:three_prime_UTR
ACTCAGAATCTCCCAAGGAC+CGG 0.572963 3_1:+77181544 Msa0376860:five_prime_UTR
GTCTGTTAGCTATTTATAGA+GGG 0.573849 3_1:+77183988 Msa0376860:three_prime_UTR
GGATCATCCCGGAGGTGATG+AGG 0.581222 3_1:+77183571 Msa0376860:CDS
AACAGAACCTCATCACCTCC+GGG 0.588122 3_1:-77183578 None:intergenic
GTACAATCCGGACAAGACTT+CGG 0.598055 3_1:+77183840 Msa0376860:CDS
GGAACGTAGGTGCGCTTTAG+GGG 0.600026 3_1:-77183802 None:intergenic
TCAGCAATCTCAGTACAATC+CGG 0.604833 3_1:+77183828 Msa0376860:CDS
ATGATGGACAAATACTACAT+TGG 0.610366 3_1:+77183757 Msa0376860:CDS
TAGCTAACAGACCCCTCACA+AGG 0.620015 3_1:-77183977 None:intergenic
TTGTACTGAGATTGCTGAGG+TGG 0.630347 3_1:-77183823 None:intergenic
GGTGTAGTTTCTGACAACAA+AGG 0.643712 3_1:-77183917 None:intergenic
TCATATTGCACCCTTGTGAG+GGG 0.653808 3_1:+77183966 Msa0376860:three_prime_UTR
TCATCACTCAGAATCTCCCA+AGG 0.658723 3_1:+77181539 Msa0376860:five_prime_UTR
GCTAAGCCCAATATCAGGAG+AGG 0.665710 3_1:-77183895 None:intergenic
GGTGCGCTTTAGGGGAACAG+TGG 0.670761 3_1:-77183794 None:intergenic
GGGATGATCCTCCATAAAAG+GGG 0.676795 3_1:-77183558 None:intergenic
AGCTAACAGACCCCTCACAA+GGG 0.690074 3_1:-77183976 None:intergenic
GGATTGTACTGAGATTGCTG+AGG 0.741733 3_1:-77183826 None:intergenic
TTTATGGAGGATCATCCCGG+AGG 0.751420 3_1:+77183563 Msa0376860:CDS
AGATTGCTGAGGTGGAACGT+AGG 0.774863 3_1:-77183815 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTAAAGAATCAATAAAAA+GGG - chr3_1:77181728-77181747 None:intergenic 15.0%
!!! ATTCAGATAAAATTAATTTG+TGG + chr3_1:77181760-77181779 Msa0376860:intron 15.0%
!!! TTCAGATAAAATTAATTTGT+GGG + chr3_1:77181761-77181780 Msa0376860:intron 15.0%
!! AGTTAAAATATTAGTACATA+TGG - chr3_1:77182732-77182751 None:intergenic 15.0%
!!! TATATTACATTTGTATTTTG+TGG + chr3_1:77182845-77182864 Msa0376860:intron 15.0%
!!! ATATTACATTTGTATTTTGT+GGG + chr3_1:77182846-77182865 Msa0376860:intron 15.0%
!! ATAAATTAATAACCCATTAT+AGG - chr3_1:77183073-77183092 None:intergenic 15.0%
!! ATAGTACAATATAATCAATA+TGG - chr3_1:77183665-77183684 None:intergenic 15.0%
!!! TGATCCTTTAAAAAAAAAAA+AGG - chr3_1:77184195-77184214 None:intergenic 15.0%
!! TCTAAAGAATCAATAAAAAG+GGG - chr3_1:77181727-77181746 None:intergenic 20.0%
!! GATCTAAAGAATCAATAAAA+AGG - chr3_1:77181729-77181748 None:intergenic 20.0%
!! ACTACAAATGAATTACAATT+AGG - chr3_1:77182134-77182153 None:intergenic 20.0%
!! CAACATTCAATCTTAAAATT+AGG - chr3_1:77182205-77182224 None:intergenic 20.0%
!! TTGAATTATTCAAATCTGAA+CGG + chr3_1:77182491-77182510 Msa0376860:intron 20.0%
!!! TGATGACTCATTTTATTATT+TGG - chr3_1:77182563-77182582 None:intergenic 20.0%
!!! TATTACATTTGTATTTTGTG+GGG + chr3_1:77182847-77182866 Msa0376860:intron 20.0%
!! TAAGTTAATTTACCTCTTAT+TGG + chr3_1:77182985-77183004 Msa0376860:intron 20.0%
!!! TTATTGGTATCCTTTTAAAA+TGG + chr3_1:77183001-77183020 Msa0376860:intron 20.0%
!! CGTAGAAAAATTTATGTATT+TGG - chr3_1:77183453-77183472 None:intergenic 20.0%
!!! TATAATGTTTTACAACTTTC+TGG + chr3_1:77184067-77184086 Msa0376860:three_prime_UTR 20.0%
!!! CAAGCCTTTTTTTTTTTTAA+AGG + chr3_1:77184188-77184207 Msa0376860:three_prime_UTR 20.0%
! TTCCATGATTGATTATTCTT+CGG + chr3_1:77181949-77181968 Msa0376860:intron 25.0%
!!! AAGATGAAGTTCAAGTTTTT+GGG + chr3_1:77182052-77182071 Msa0376860:intron 25.0%
!! TTTAAGATTGAATGTTGTTG+AGG + chr3_1:77182208-77182227 Msa0376860:intron 25.0%
!!! GTAGACAGATTACATTTTTT+AGG + chr3_1:77182295-77182314 Msa0376860:intron 25.0%
! TACAATAAGATCCAAACTTA+AGG - chr3_1:77182466-77182485 None:intergenic 25.0%
! TTTAACTGTCACGTAAAATA+TGG + chr3_1:77182745-77182764 Msa0376860:intron 25.0%
! AATGTAATATAAGAGACTCT+CGG - chr3_1:77182836-77182855 None:intergenic 25.0%
! TTTAAAAGGATACCAATAAG+AGG - chr3_1:77183000-77183019 None:intergenic 25.0%
! AAGCATCTAAGCTATATAAA+AGG - chr3_1:77183233-77183252 None:intergenic 25.0%
!! AACGTTTAAAATAGTGAAAG+TGG - chr3_1:77183338-77183357 None:intergenic 25.0%
! AAGAACAAATTATCGTTAAG+AGG - chr3_1:77183513-77183532 None:intergenic 25.0%
! ATTTGTTCTTCAATCATGTT+AGG + chr3_1:77183523-77183542 Msa0376860:intron 25.0%
! TATGCTTATATCATTCGAAA+AGG + chr3_1:77183613-77183632 Msa0376860:intron 25.0%
! ATGCTTATATCATTCGAAAA+GGG + chr3_1:77183614-77183633 Msa0376860:intron 25.0%
! TACTATACTGTATCAATTAC+AGG + chr3_1:77183679-77183698 Msa0376860:intron 25.0%
! ACTATACTGTATCAATTACA+GGG + chr3_1:77183680-77183699 Msa0376860:intron 25.0%
! ACTGAAAAACCTAATAATGT+TGG + chr3_1:77184112-77184131 Msa0376860:three_prime_UTR 25.0%
! ATTCACAATCCAACATTATT+AGG - chr3_1:77184124-77184143 None:intergenic 25.0%
TCAAATCTCTTCTTCACTTT+GGG - chr3_1:77181503-77181522 None:intergenic 30.0%
TTTGAAGGGAAAAAACAAGA+GGG - chr3_1:77181581-77181600 None:intergenic 30.0%
ATTTGAAGGGAAAAAACAAG+AGG - chr3_1:77181582-77181601 None:intergenic 30.0%
GGAAAAGAGAAGAATTTGAA+GGG - chr3_1:77181595-77181614 None:intergenic 30.0%
TAGAAAAGAATCGATGCAAA+GGG - chr3_1:77181616-77181635 None:intergenic 30.0%
TTAGAAAAGAATCGATGCAA+AGG - chr3_1:77181617-77181636 None:intergenic 30.0%
!!! GGTTTGACTTGAATTTTTCT+GGG + chr3_1:77181782-77181801 Msa0376860:intron 30.0%
TGGCAGTGAAAGAAAAAAAA+TGG + chr3_1:77181817-77181836 Msa0376860:intron 30.0%
TTCCGAAGAATAATCAATCA+TGG - chr3_1:77181954-77181973 None:intergenic 30.0%
!! GAAGATGAAGTTCAAGTTTT+TGG + chr3_1:77182051-77182070 Msa0376860:intron 30.0%
TGCATGTTACATATATTTCC+TGG + chr3_1:77182079-77182098 Msa0376860:intron 30.0%
TCAGTGTGGTTAACAAATTA+TGG + chr3_1:77182253-77182272 Msa0376860:intron 30.0%
AATAAGATCCAAACTTAAGG+TGG - chr3_1:77182463-77182482 None:intergenic 30.0%
ATGTAATATAAGAGACTCTC+GGG - chr3_1:77182835-77182854 None:intergenic 30.0%
!!! TTGGTATCCTTTTAAAATGG+AGG + chr3_1:77183004-77183023 Msa0376860:intron 30.0%
!!! TGGTATCCTTTTAAAATGGA+GGG + chr3_1:77183005-77183024 Msa0376860:intron 30.0%
AATAACCCATTATAGGAAGA+CGG - chr3_1:77183066-77183085 None:intergenic 30.0%
TAAAGAATCAAACTGTGGAA+AGG - chr3_1:77183109-77183128 None:intergenic 30.0%
AAGGATAAAGAATCAAACTG+TGG - chr3_1:77183114-77183133 None:intergenic 30.0%
GCTATATAAAAGGAAGATTG+AGG - chr3_1:77183223-77183242 None:intergenic 30.0%
TATAGCTTAGATGCTTTCAT+TGG + chr3_1:77183237-77183256 Msa0376860:intron 30.0%
TCTTATAGGAAGATAGGTTA+GGG - chr3_1:77183282-77183301 None:intergenic 30.0%
CTAATGTCTTATAGGAAGAT+AGG - chr3_1:77183288-77183307 None:intergenic 30.0%
AATTAGAATAGCCTCACAAT+TGG - chr3_1:77183378-77183397 None:intergenic 30.0%
!! AACCAATGATTTTGAAGATG+TGG + chr3_1:77183711-77183730 Msa0376860:CDS 30.0%
!! ACCAATGATTTTGAAGATGT+GGG + chr3_1:77183712-77183731 Msa0376860:CDS 30.0%
!! ATGATGGACAAATACTACAT+TGG + chr3_1:77183757-77183776 Msa0376860:CDS 30.0%
GTGCAATATGATGATAAAGA+AGG - chr3_1:77183957-77183976 None:intergenic 30.0%
GTCTGTTAGCTATTTATAGA+GGG + chr3_1:77183988-77184007 Msa0376860:three_prime_UTR 30.0%
ATTTATAGAGGGTCATTCAA+AGG + chr3_1:77183999-77184018 Msa0376860:three_prime_UTR 30.0%
!! GTTTTACAACTTTCTGGTAA+TGG + chr3_1:77184073-77184092 Msa0376860:three_prime_UTR 30.0%
! GTTTAGCATCTTTGTTTCAT+TGG + chr3_1:77184157-77184176 Msa0376860:three_prime_UTR 30.0%
! ACCCTTAATTTTCCTGTTTA+TGG + chr3_1:77184248-77184267 Msa0376860:three_prime_UTR 30.0%
CCCATAAACAGGAAAATTAA+GGG - chr3_1:77184252-77184271 None:intergenic 30.0%
! CCCTTAATTTTCCTGTTTAT+GGG + chr3_1:77184249-77184268 Msa0376860:three_prime_UTR 30.0%
! CCTTAATTTTCCTGTTTATG+GGG + chr3_1:77184250-77184269 Msa0376860:three_prime_UTR 30.0%
CAAATCTCTTCTTCACTTTG+GGG - chr3_1:77181502-77181521 None:intergenic 35.0%
GTCAAATCTCTTCTTCACTT+TGG - chr3_1:77181504-77181523 None:intergenic 35.0%
GGGAAAAGAGAAGAATTTGA+AGG - chr3_1:77181596-77181615 None:intergenic 35.0%
!!! GGGTTTGACTTGAATTTTTC+TGG + chr3_1:77181781-77181800 Msa0376860:intron 35.0%
!! TTTCTGGGTCTTTGATTTTG+TGG + chr3_1:77181797-77181816 Msa0376860:intron 35.0%
TTTGGTCATCTATGAGTAAG+AGG - chr3_1:77182545-77182564 None:intergenic 35.0%
CATGCAAAATTACTGGTGTT+AGG - chr3_1:77182605-77182624 None:intergenic 35.0%
TCTCTAGCATGCAAAATTAC+TGG - chr3_1:77182612-77182631 None:intergenic 35.0%
! TAATTTTGCATGCTAGAGAG+TGG + chr3_1:77182613-77182632 Msa0376860:intron 35.0%
GAGTAGAAGAATATAGCATC+TGG + chr3_1:77182638-77182657 Msa0376860:intron 35.0%
ACGACTAGTAATGCACATAA+AGG - chr3_1:77182695-77182714 None:intergenic 35.0%
!!! TATCCTTTTAAAATGGAGGG+AGG + chr3_1:77183008-77183027 Msa0376860:intron 35.0%
! TTTCCTCCCTCCATTTTAAA+AGG - chr3_1:77183014-77183033 None:intergenic 35.0%
AAAATGGAGGGAGGAAAAAA+AGG + chr3_1:77183017-77183036 Msa0376860:intron 35.0%
ATAAACCCGTCTTCCTATAA+TGG + chr3_1:77183057-77183076 Msa0376860:intron 35.0%
TAAACCCGTCTTCCTATAAT+GGG + chr3_1:77183058-77183077 Msa0376860:intron 35.0%
ATAACCCATTATAGGAAGAC+GGG - chr3_1:77183065-77183084 None:intergenic 35.0%
! TTAGTCAAATATGCTCTGAG+AGG + chr3_1:77183190-77183209 Msa0376860:intron 35.0%
CTTATAGGAAGATAGGTTAG+GGG - chr3_1:77183281-77183300 None:intergenic 35.0%
GTCTTATAGGAAGATAGGTT+AGG - chr3_1:77183283-77183302 None:intergenic 35.0%
! TCGACTAGCTAATGTCTTAT+AGG - chr3_1:77183296-77183315 None:intergenic 35.0%
TCCCACATCTTCAAAATCAT+TGG - chr3_1:77183716-77183735 None:intergenic 35.0%
TGATTCTGCTAGAGAAATGA+TGG + chr3_1:77183741-77183760 Msa0376860:CDS 35.0%
! GATCAATATTTTGCAGTTCC+TGG + chr3_1:77183870-77183889 Msa0376860:CDS 35.0%
AAGAAGGAACTACTCTTTCT+TGG - chr3_1:77183941-77183960 None:intergenic 35.0%
GGTCTGTTAGCTATTTATAG+AGG + chr3_1:77183987-77184006 Msa0376860:three_prime_UTR 35.0%
! GACAGTGTTTAGTTTCTTCT+TGG + chr3_1:77184021-77184040 Msa0376860:three_prime_UTR 35.0%
CCCCATAAACAGGAAAATTA+AGG - chr3_1:77184253-77184272 None:intergenic 35.0%
! TCATCGAAAGTGTGAAGCTT+TGG - chr3_1:77181851-77181870 None:intergenic 40.0%
ACAACAAGACCAAAGATTGC+TGG + chr3_1:77181882-77181901 Msa0376860:CDS 40.0%
GAGAATAAGCCAGCAATCTT+TGG - chr3_1:77181894-77181913 None:intergenic 40.0%
ATGCTATGTCTAGATCAGTG+TGG + chr3_1:77182239-77182258 Msa0376860:intron 40.0%
GTCTACAAGTACAGAAGAAC+AGG - chr3_1:77182281-77182300 None:intergenic 40.0%
ACAAACGAAGCATGTCATGT+AGG - chr3_1:77182434-77182453 None:intergenic 40.0%
AAGATCCAAACTTAAGGTGG+TGG - chr3_1:77182460-77182479 None:intergenic 40.0%
AGATAGATCTCAAGATCTGC+AGG - chr3_1:77182927-77182946 None:intergenic 40.0%
ATCTGATGTTTGATGCCATG+TGG + chr3_1:77182943-77182962 Msa0376860:intron 40.0%
TCTGATGTTTGATGCCATGT+GGG + chr3_1:77182944-77182963 Msa0376860:intron 40.0%
! ATAGGAAGACGGGTTTATGA+AGG - chr3_1:77183055-77183074 None:intergenic 40.0%
CTAGTCGAAAATCTAGCAGA+AGG + chr3_1:77183308-77183327 Msa0376860:intron 40.0%
! ATGGTGCTCTGCATTTTTCA+GGG - chr3_1:77183401-77183420 None:intergenic 40.0%
! ATGCAGAGCACCATGTATAT+TGG + chr3_1:77183407-77183426 Msa0376860:intron 40.0%
! ACCATGTATATTGGCCTGAA+TGG + chr3_1:77183416-77183435 Msa0376860:intron 40.0%
ACCATTCAGGCCAATATACA+TGG - chr3_1:77183420-77183439 None:intergenic 40.0%
TCAGCAATCTCAGTACAATC+CGG + chr3_1:77183828-77183847 Msa0376860:CDS 40.0%
! ACAAATTCCGAAGTCTTGTC+CGG - chr3_1:77183850-77183869 None:intergenic 40.0%
GGTGTAGTTTCTGACAACAA+AGG - chr3_1:77183920-77183939 None:intergenic 40.0%
ATCATATTGCACCCTTGTGA+GGG + chr3_1:77183965-77183984 Msa0376860:three_prime_UTR 40.0%
!! CAACTTTCTGGTAATGGTAC+TGG + chr3_1:77184079-77184098 Msa0376860:three_prime_UTR 40.0%
!! TCTCTTCTTCACTTTGGGGT+TGG - chr3_1:77181498-77181517 None:intergenic 45.0%
TCATCACTCAGAATCTCCCA+AGG + chr3_1:77181539-77181558 Msa0376860:five_prime_UTR 45.0%
AATTACCACTTCCGGTCCTT+GGG - chr3_1:77181558-77181577 None:intergenic 45.0%
CAAGAGGGAATTACCACTTC+CGG - chr3_1:77181566-77181585 None:intergenic 45.0%
!! GATTGCTGGCTTATTCTCTC+TGG + chr3_1:77181896-77181915 Msa0376860:CDS 45.0%
! CTGGCTTATTCTCTCTGGAA+AGG + chr3_1:77181901-77181920 Msa0376860:CDS 45.0%
ACATGCAATGCACAAGATCC+AGG - chr3_1:77182100-77182119 None:intergenic 45.0%
GTCTACCACCACCTTAAGTT+TGG + chr3_1:77182452-77182471 Msa0376860:intron 45.0%
GTGCATTACTAGTCGTGTAC+TGG + chr3_1:77182699-77182718 Msa0376860:intron 45.0%
CTGCTCGCTTATGAATGGTA+TGG - chr3_1:77182782-77182801 None:intergenic 45.0%
CGATACTGCTCGCTTATGAA+TGG - chr3_1:77182787-77182806 None:intergenic 45.0%
TTTGATGCCATGTGGGACTT+GGG + chr3_1:77182951-77182970 Msa0376860:intron 45.0%
AACTGTGGAAAGGACGAGTA+TGG - chr3_1:77183099-77183118 None:intergenic 45.0%
CATTGTGTTTCGACGTCACA+GGG - chr3_1:77183142-77183161 None:intergenic 45.0%
CTGTGACGTCGAAACACAAT+GGG + chr3_1:77183141-77183160 Msa0376860:intron 45.0%
TGTGACGTCGAAACACAATG+GGG + chr3_1:77183142-77183161 Msa0376860:intron 45.0%
! AAACACAATGGGGAGAGTGT+TGG + chr3_1:77183152-77183171 Msa0376860:intron 45.0%
!! GTCAAATATGCTCTGAGAGG+TGG + chr3_1:77183193-77183212 Msa0376860:intron 45.0%
TAGGAAGATAGGTTAGGGGA+AGG - chr3_1:77183277-77183296 None:intergenic 45.0%
AACGTTGCCTGATATTGCGA+TGG + chr3_1:77183352-77183371 Msa0376860:intron 45.0%
CAATTGGCCATCGCAATATC+AGG - chr3_1:77183362-77183381 None:intergenic 45.0%
TATTGCGATGGCCAATTGTG+AGG + chr3_1:77183364-77183383 Msa0376860:intron 45.0%
! CATGGTGCTCTGCATTTTTC+AGG - chr3_1:77183402-77183421 None:intergenic 45.0%
TTGGCCTGAATGGTCACTAT+TGG + chr3_1:77183426-77183445 Msa0376860:intron 45.0%
TGGCCTGAATGGTCACTATT+GGG + chr3_1:77183427-77183446 Msa0376860:intron 45.0%
! GTACGATGTCACCCCTTTTA+TGG + chr3_1:77183547-77183566 Msa0376860:CDS 45.0%
GGGATGATCCTCCATAAAAG+GGG - chr3_1:77183561-77183580 None:intergenic 45.0%
CGGGATGATCCTCCATAAAA+GGG - chr3_1:77183562-77183581 None:intergenic 45.0%
! CCTTTTATGGAGGATCATCC+CGG + chr3_1:77183560-77183579 Msa0376860:CDS 45.0%
!! GGTATGCTACTAAAGCACTG+AGG + chr3_1:77183635-77183654 Msa0376860:intron 45.0%
TTGTACTGAGATTGCTGAGG+TGG - chr3_1:77183826-77183845 None:intergenic 45.0%
GGATTGTACTGAGATTGCTG+AGG - chr3_1:77183829-77183848 None:intergenic 45.0%
GTACAATCCGGACAAGACTT+CGG + chr3_1:77183840-77183859 Msa0376860:CDS 45.0%
CAAAGGCTAAGCCCAATATC+AGG - chr3_1:77183903-77183922 None:intergenic 45.0%
CATCATATTGCACCCTTGTG+AGG + chr3_1:77183964-77183983 Msa0376860:three_prime_UTR 45.0%
TCATATTGCACCCTTGTGAG+GGG + chr3_1:77183966-77183985 Msa0376860:three_prime_UTR 45.0%
!! GGTGTCTTGAGCATTGAAAC+TGG + chr3_1:77184042-77184061 Msa0376860:three_prime_UTR 45.0%
CACTTTGCATCCCCATAAAC+AGG - chr3_1:77184263-77184282 None:intergenic 45.0%
ACTCAGAATCTCCCAAGGAC+CGG + chr3_1:77181544-77181563 Msa0376860:five_prime_UTR 50.0%
GAATTACCACTTCCGGTCCT+TGG - chr3_1:77181559-77181578 None:intergenic 50.0%
GTTTGATGCCATGTGGGACT+TGG + chr3_1:77182950-77182969 Msa0376860:intron 50.0%
CCATTGTGTTTCGACGTCAC+AGG - chr3_1:77183143-77183162 None:intergenic 50.0%
CCTGTGACGTCGAAACACAA+TGG + chr3_1:77183140-77183159 Msa0376860:intron 50.0%
TGGCCCAATAGTGACCATTC+AGG - chr3_1:77183433-77183452 None:intergenic 50.0%
! CGATGTCACCCCTTTTATGG+AGG + chr3_1:77183550-77183569 Msa0376860:CDS 50.0%
CCGGGATGATCCTCCATAAA+AGG - chr3_1:77183563-77183582 None:intergenic 50.0%
TTTATGGAGGATCATCCCGG+AGG + chr3_1:77183563-77183582 Msa0376860:CDS 50.0%
AACAGAACCTCATCACCTCC+GGG - chr3_1:77183581-77183600 None:intergenic 50.0%
CAACAGAACCTCATCACCTC+CGG - chr3_1:77183582-77183601 None:intergenic 50.0%
GAGGTTCTGTTGTCTGCAAC+AGG + chr3_1:77183590-77183609 Msa0376860:CDS 50.0%
TGGAACGTAGGTGCGCTTTA+GGG - chr3_1:77183806-77183825 None:intergenic 50.0%
AGATTGCTGAGGTGGAACGT+AGG - chr3_1:77183818-77183837 None:intergenic 50.0%
CTGGTGCCTCTCCTGATATT+GGG + chr3_1:77183889-77183908 Msa0376860:CDS 50.0%
GCTAAGCCCAATATCAGGAG+AGG - chr3_1:77183898-77183917 None:intergenic 50.0%
AGCTAACAGACCCCTCACAA+GGG - chr3_1:77183979-77183998 None:intergenic 50.0%
TAGCTAACAGACCCCTCACA+AGG - chr3_1:77183980-77183999 None:intergenic 50.0%
AATCTCCCAAGGACCGGAAG+TGG + chr3_1:77181550-77181569 Msa0376860:five_prime_UTR 55.0%
ATGCCATGTGGGACTTGGGA+AGG + chr3_1:77182955-77182974 Msa0376860:intron 55.0%
TGCCATGTGGGACTTGGGAA+GGG + chr3_1:77182956-77182975 Msa0376860:intron 55.0%
TTCCCTTCCCAAGTCCCACA+TGG - chr3_1:77182961-77182980 None:intergenic 55.0%
TCGACGTCACAGGGAACAGA+AGG - chr3_1:77183133-77183152 None:intergenic 55.0%
!! CGCTTTAGGGGAACAGTGGA+TGG - chr3_1:77183793-77183812 None:intergenic 55.0%
GGAACGTAGGTGCGCTTTAG+GGG - chr3_1:77183805-77183824 None:intergenic 55.0%
GTGGAACGTAGGTGCGCTTT+AGG - chr3_1:77183807-77183826 None:intergenic 55.0%
!! CCAATATCAGGAGAGGCACC+AGG - chr3_1:77183891-77183910 None:intergenic 55.0%
CCTGGTGCCTCTCCTGATAT+TGG + chr3_1:77183888-77183907 Msa0376860:CDS 55.0%
GGATCATCCCGGAGGTGATG+AGG + chr3_1:77183571-77183590 Msa0376860:CDS 60.0%
!! GGTGCGCTTTAGGGGAACAG+TGG - chr3_1:77183797-77183816 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 77181494 77184307 77181494 ID=Msa0376860;Name=Msa0376860
chr3_1 mRNA 77181494 77184307 77181494 ID=Msa0376860-mRNA-1;Parent=Msa0376860;Name=Msa0376860-mRNA-1;_AED=0.01;_eAED=0.01;_QI=91|1|1|1|0.66|0.5|4|356|134
chr3_1 exon 77181494 77181571 77181494 ID=Msa0376860-mRNA-1:exon:18359;Parent=Msa0376860-mRNA-1
chr3_1 exon 77181823 77181922 77181823 ID=Msa0376860-mRNA-1:exon:18360;Parent=Msa0376860-mRNA-1
chr3_1 exon 77183545 77183611 77183545 ID=Msa0376860-mRNA-1:exon:18361;Parent=Msa0376860-mRNA-1
chr3_1 exon 77183701 77184307 77183701 ID=Msa0376860-mRNA-1:exon:18362;Parent=Msa0376860-mRNA-1
chr3_1 five_prime_UTR 77181494 77181571 77181494 ID=Msa0376860-mRNA-1:five_prime_utr;Parent=Msa0376860-mRNA-1
chr3_1 five_prime_UTR 77181823 77181835 77181823 ID=Msa0376860-mRNA-1:five_prime_utr;Parent=Msa0376860-mRNA-1
chr3_1 CDS 77181836 77181922 77181836 ID=Msa0376860-mRNA-1:cds;Parent=Msa0376860-mRNA-1
chr3_1 CDS 77183545 77183611 77183545 ID=Msa0376860-mRNA-1:cds;Parent=Msa0376860-mRNA-1
chr3_1 CDS 77183701 77183951 77183701 ID=Msa0376860-mRNA-1:cds;Parent=Msa0376860-mRNA-1
chr3_1 three_prime_UTR 77183952 77184307 77183952 ID=Msa0376860-mRNA-1:three_prime_utr;Parent=Msa0376860-mRNA-1
Gene Sequence

>Msa0376860

ATGGCTTCAGATCCAAAGCTTCACACTTTCGATGATGTGTCAAAGCACAACAAGACCAAAGATTGCTGGCTTATTCTCTCTGGAAAGGTGTACGATGTCACCCCTTTTATGGAGGATCATCCCGGAGGTGATGAGGTTCTGTTGTCTGCAACAGGGAAAGATGCAACCAATGATTTTGAAGATGTGGGACACAGTGATTCTGCTAGAGAAATGATGGACAAATACTACATTGGTGAGATTGATCCATCCACTGTTCCCCTAAAGCGCACCTACGTTCCACCTCAGCAATCTCAGTACAATCCGGACAAGACTTCGGAATTTGTGATCAATATTTTGCAGTTCCTGGTGCCTCTCCTGATATTGGGCTTAGCCTTTGTTGTCAGAAACTACACCAAGAAAGAGTAG
Protein sequence

>Msa0376860

MASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVINILQFLVPLLILGLAFVVRNYTKKE*