AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0577690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577690 MtrunA17_Chr8g0377461 98.148 54 1 0 1 54 1 54 5.44e-33 108
Msa0577690 MtrunA17_Chr2g0290301 53.704 54 18 1 1 54 1 47 9.39e-15 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0577690 AT1G27350.1 83.333 54 9 0 1 54 1 54 2.53e-27 94.4
Msa0577690 AT1G27330.1 83.333 54 9 0 1 54 1 54 2.53e-27 94.4

Find 49 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTAATATCATGAAAATTT+TGG 0.147132 4_1:+82028312 Msa0577690:three_prime_UTR
TTATTAAATTCAACAAAATT+AGG 0.206186 4_1:+82026133 None:intergenic
TCAACAAAATTAGGCATATT+AGG 0.209880 4_1:+82026142 Msa0577690:five_prime_UTR
CCTTTCTTAACAGTTGTTTC+TGG 0.223249 4_1:-82026836 None:intergenic
ATGATTGATTTGTAGGATAA+TGG 0.270299 4_1:-82026340 None:intergenic
TTCTTCGTCTTTGTTGTCAT+TGG 0.272950 4_1:+82026893 Msa0577690:CDS
ACGAACCATGATTGATTTGT+AGG 0.306992 4_1:-82026347 None:intergenic
TTGTGCACCATACACAAGTT+TGG 0.320524 4_1:+82028110 Msa0577690:three_prime_UTR
AAAGTCATTGTTTGTATTGA+TGG 0.322469 4_1:-82028257 None:intergenic
ACGAAGAAACCAAGCAAAAC+GGG 0.342450 4_1:-82026878 None:intergenic
TGTGCACCATACACAAGTTT+GGG 0.352272 4_1:+82028111 Msa0577690:three_prime_UTR
CAGCTCTCTTTCAGATAATC+AGG 0.365308 4_1:+82027951 Msa0577690:intron
CCAAGCAAAACGGGGCCAAC+AGG 0.391773 4_1:-82026869 None:intergenic
GACGAAGAAACCAAGCAAAA+CGG 0.413008 4_1:-82026879 None:intergenic
TGAACGGTGATCGTTGTTAC+CGG 0.416731 4_1:-82026207 None:intergenic
AACTTTCCCAAACTTGTGTA+TGG 0.418198 4_1:-82028117 None:intergenic
CATCAAAGCGCCTTGCAGTT+AGG 0.424154 4_1:+82026768 Msa0577690:CDS
GGCATAATGGAGCTTTGAGT+TGG 0.445961 4_1:+82027994 Msa0577690:exon
CGAGTGGAGGCATGGCATAA+TGG 0.448620 4_1:+82027981 Msa0577690:CDS
AAAAGTTGGAAATAAATAAC+CGG 0.474202 4_1:+82026188 Msa0577690:five_prime_UTR
TATTGATGGTGGTGGGTCAA+TGG 0.489118 4_1:-82028243 None:intergenic
ATTGTTTGTATTGATGGTGG+TGG 0.492173 4_1:-82028251 None:intergenic
TTTCTACATGTACTTGGAGG+TGG 0.493145 4_1:+82028062 Msa0577690:three_prime_UTR
TGGTGGTGGGTCAATGGCAG+AGG 0.494395 4_1:-82028237 None:intergenic
AATTAGGCATATTAGGTAAG+TGG 0.504893 4_1:+82026149 Msa0577690:five_prime_UTR
CCAGAAACAACTGTTAAGAA+AGG 0.524644 4_1:+82026836 Msa0577690:CDS
TTGTTTGTATTGATGGTGGT+GGG 0.528071 4_1:-82028250 None:intergenic
TGGTGGGTCAATGGCAGAGG+TGG 0.533554 4_1:-82028234 None:intergenic
ATTATCCTACAAATCAATCA+TGG 0.554540 4_1:+82026342 Msa0577690:exon
GGCAGAGGTGGAAGATTGAA+AGG 0.556793 4_1:-82028222 None:intergenic
GTCATTGTTTGTATTGATGG+TGG 0.558487 4_1:-82028254 None:intergenic
TAAGGAGGACAACATTGAAA+GGG 0.568218 4_1:-82026930 None:intergenic
ATAAGGAGGACAACATTGAA+AGG 0.568330 4_1:-82026931 None:intergenic
AAAGGAAATGACTATCCTGT+TGG 0.571369 4_1:+82026854 Msa0577690:CDS
GACAGCAACGAGTGGAGGCA+TGG 0.571659 4_1:+82027973 Msa0577690:CDS
AAAGCGCCTTGCAGTTAGGA+AGG 0.575086 4_1:+82026772 Msa0577690:CDS
ATGATATTACAGATATGGAA+AGG 0.577838 4_1:-82028301 None:intergenic
TTTGTAGGATAATGGATCGC+CGG 0.595314 4_1:-82026332 None:intergenic
ACCTCGGAGAGAGTCACTGC+CGG 0.598531 4_1:+82026256 Msa0577690:five_prime_UTR
ATCAGGACAGCAACGAGTGG+AGG 0.608196 4_1:+82027968 Msa0577690:CDS
CGAAGAAACCAAGCAAAACG+GGG 0.617394 4_1:-82026877 None:intergenic
TCCGGCAGTGACTCTCTCCG+AGG 0.626564 4_1:-82026257 None:intergenic
GAAATCTCTAAATAAAACCT+CGG 0.627274 4_1:+82026240 Msa0577690:five_prime_UTR
TCTCAACCTTCCTAACTGCA+AGG 0.647474 4_1:-82026778 None:intergenic
GATTTCTGAATATCTGTGAA+CGG 0.648636 4_1:-82026223 None:intergenic
ATAATCAGGACAGCAACGAG+TGG 0.658965 4_1:+82027965 Msa0577690:CDS
GTAGGATAATGGATCGCCGG+TGG 0.665340 4_1:-82026329 None:intergenic
AACGCCGGAAAATTGAAAGA+CGG 0.693395 4_1:-82026294 None:intergenic
GCCGGAAAATTGAAAGACGG+TGG 0.699767 4_1:-82026291 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAAAAAAAATTACAG+GGG - chr4_1:82027277-82027296 None:intergenic 10.0%
!! GTAAATAAAAAAAAAATTAC+AGG - chr4_1:82027279-82027298 None:intergenic 10.0%
!! AAATAAAAAAAAAATTACAG+GGG - chr4_1:82027277-82027296 None:intergenic 10.0%
!! GTAAATAAAAAAAAAATTAC+AGG - chr4_1:82027279-82027298 None:intergenic 10.0%
!! TCAATTTCAAAACAAAAATT+CGG - chr4_1:82026497-82026516 None:intergenic 15.0%
!! ATCAGAAATTTATCAAAATT+AGG + chr4_1:82026560-82026579 Msa0577690:intron 15.0%
!!! TGTTAAATTTTGATTGAATT+TGG + chr4_1:82026682-82026701 Msa0577690:intron 15.0%
!! AAAAATTAGTAAAACACATA+AGG - chr4_1:82026951-82026970 None:intergenic 15.0%
!! CAATATTTCAAAAATTGAAT+CGG - chr4_1:82027201-82027220 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG + chr4_1:82027205-82027224 Msa0577690:intron 15.0%
!! AATAAAAAAAAAATTACAGG+GGG - chr4_1:82027276-82027295 None:intergenic 15.0%
!!! TATATTAATGTGTTTGAAAT+TGG - chr4_1:82027410-82027429 None:intergenic 15.0%
!!! ATAATTTTCAATTCAATTCA+AGG - chr4_1:82027493-82027512 None:intergenic 15.0%
!! TCAATTTCAAAACAAAAATT+CGG - chr4_1:82026497-82026516 None:intergenic 15.0%
!! ATCAGAAATTTATCAAAATT+AGG + chr4_1:82026560-82026579 Msa0577690:intron 15.0%
!!! TGTTAAATTTTGATTGAATT+TGG + chr4_1:82026682-82026701 Msa0577690:intron 15.0%
!! AAAAATTAGTAAAACACATA+AGG - chr4_1:82026951-82026970 None:intergenic 15.0%
!! CAATATTTCAAAAATTGAAT+CGG - chr4_1:82027201-82027220 None:intergenic 15.0%
!!! AATTTTTGAAATATTGTTGT+GGG + chr4_1:82027205-82027224 Msa0577690:intron 15.0%
!! AATAAAAAAAAAATTACAGG+GGG - chr4_1:82027276-82027295 None:intergenic 15.0%
!!! TATATTAATGTGTTTGAAAT+TGG - chr4_1:82027410-82027429 None:intergenic 15.0%
!!! ATAATTTTCAATTCAATTCA+AGG - chr4_1:82027493-82027512 None:intergenic 15.0%
!! AAAAGTTGGAAATAAATAAC+CGG + chr4_1:82026188-82026207 Msa0577690:five_prime_UTR 20.0%
!!! TTACATTGATTTTTAGCTTA+TGG + chr4_1:82026616-82026635 Msa0577690:intron 20.0%
!! TATTGTTTGTGTGATTTATT+TGG + chr4_1:82027020-82027039 Msa0577690:intron 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG + chr4_1:82027204-82027223 Msa0577690:intron 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG + chr4_1:82027206-82027225 Msa0577690:intron 20.0%
!! AAAACAAAAAATTTGTACAG+GGG - chr4_1:82027301-82027320 None:intergenic 20.0%
!! CAAAACAAAAAATTTGTACA+GGG - chr4_1:82027302-82027321 None:intergenic 20.0%
!! TCAAAACAAAAAATTTGTAC+AGG - chr4_1:82027303-82027322 None:intergenic 20.0%
!!! AAGAAATTTTCGTTTTTGTT+TGG - chr4_1:82027342-82027361 None:intergenic 20.0%
!!! GTAGTTTCATTATCATTTTA+AGG + chr4_1:82027759-82027778 Msa0577690:intron 20.0%
!!! TTTTCATGATATTACAGATA+TGG - chr4_1:82028309-82028328 None:intergenic 20.0%
!! AAAAGTTGGAAATAAATAAC+CGG + chr4_1:82026188-82026207 Msa0577690:five_prime_UTR 20.0%
!!! TTACATTGATTTTTAGCTTA+TGG + chr4_1:82026616-82026635 Msa0577690:intron 20.0%
!! TATTGTTTGTGTGATTTATT+TGG + chr4_1:82027020-82027039 Msa0577690:intron 20.0%
!!! CAATTTTTGAAATATTGTTG+TGG + chr4_1:82027204-82027223 Msa0577690:intron 20.0%
!!! ATTTTTGAAATATTGTTGTG+GGG + chr4_1:82027206-82027225 Msa0577690:intron 20.0%
!! AAAACAAAAAATTTGTACAG+GGG - chr4_1:82027301-82027320 None:intergenic 20.0%
!! CAAAACAAAAAATTTGTACA+GGG - chr4_1:82027302-82027321 None:intergenic 20.0%
!! TCAAAACAAAAAATTTGTAC+AGG - chr4_1:82027303-82027322 None:intergenic 20.0%
!!! AAGAAATTTTCGTTTTTGTT+TGG - chr4_1:82027342-82027361 None:intergenic 20.0%
!!! GTAGTTTCATTATCATTTTA+AGG + chr4_1:82027759-82027778 Msa0577690:intron 20.0%
!!! TTTTCATGATATTACAGATA+TGG - chr4_1:82028309-82028328 None:intergenic 20.0%
! TCAACAAAATTAGGCATATT+AGG + chr4_1:82026142-82026161 Msa0577690:five_prime_UTR 25.0%
! GAAATCTCTAAATAAAACCT+CGG + chr4_1:82026240-82026259 Msa0577690:five_prime_UTR 25.0%
!!! ATGATTGATTTGTAGGATAA+TGG - chr4_1:82026343-82026362 None:intergenic 25.0%
! ATTATCCTACAAATCAATCA+TGG + chr4_1:82026342-82026361 Msa0577690:exon 25.0%
!! GTCAAATTTTATGTAGCTTA+TGG + chr4_1:82026582-82026601 Msa0577690:intron 25.0%
! TGTTGAATGAAATTGAGATT+TGG + chr4_1:82026713-82026732 Msa0577690:intron 25.0%
!!! GAAATTGAGATTTGGAATTT+TGG + chr4_1:82026721-82026740 Msa0577690:intron 25.0%
! GAGAAAAATATCACAAAAAG+AGG + chr4_1:82026806-82026825 Msa0577690:CDS 25.0%
! AATTAGTAAAACACATAAGG+AGG - chr4_1:82026948-82026967 None:intergenic 25.0%
! TAAATGACCTATATTACAGA+GGG - chr4_1:82027246-82027265 None:intergenic 25.0%
! ATAAATGACCTATATTACAG+AGG - chr4_1:82027247-82027266 None:intergenic 25.0%
! TCTGTAATATAGGTCATTTA+TGG + chr4_1:82027246-82027265 Msa0577690:intron 25.0%
! CTGAGTACATAATTTATTCT+CGG + chr4_1:82027466-82027485 Msa0577690:intron 25.0%
! TGAGTACATAATTTATTCTC+GGG + chr4_1:82027467-82027486 Msa0577690:intron 25.0%
! GTATATCAAACATTTGATCA+CGG - chr4_1:82027676-82027695 None:intergenic 25.0%
!! GTATAGTCATATTTTCTACA+GGG - chr4_1:82028041-82028060 None:intergenic 25.0%
!! AGTATAGTCATATTTTCTAC+AGG - chr4_1:82028042-82028061 None:intergenic 25.0%
!! TATACTTTTCTACATGTACT+TGG + chr4_1:82028056-82028075 Msa0577690:three_prime_UTR 25.0%
! AAAGTCATTGTTTGTATTGA+TGG - chr4_1:82028260-82028279 None:intergenic 25.0%
! ATGATATTACAGATATGGAA+AGG - chr4_1:82028304-82028323 None:intergenic 25.0%
! TCAACAAAATTAGGCATATT+AGG + chr4_1:82026142-82026161 Msa0577690:five_prime_UTR 25.0%
! GAAATCTCTAAATAAAACCT+CGG + chr4_1:82026240-82026259 Msa0577690:five_prime_UTR 25.0%
!!! ATGATTGATTTGTAGGATAA+TGG - chr4_1:82026343-82026362 None:intergenic 25.0%
! ATTATCCTACAAATCAATCA+TGG + chr4_1:82026342-82026361 Msa0577690:exon 25.0%
!! GTCAAATTTTATGTAGCTTA+TGG + chr4_1:82026582-82026601 Msa0577690:intron 25.0%
! TGTTGAATGAAATTGAGATT+TGG + chr4_1:82026713-82026732 Msa0577690:intron 25.0%
!!! GAAATTGAGATTTGGAATTT+TGG + chr4_1:82026721-82026740 Msa0577690:intron 25.0%
! GAGAAAAATATCACAAAAAG+AGG + chr4_1:82026806-82026825 Msa0577690:CDS 25.0%
! AATTAGTAAAACACATAAGG+AGG - chr4_1:82026948-82026967 None:intergenic 25.0%
! TAAATGACCTATATTACAGA+GGG - chr4_1:82027246-82027265 None:intergenic 25.0%
! ATAAATGACCTATATTACAG+AGG - chr4_1:82027247-82027266 None:intergenic 25.0%
! TCTGTAATATAGGTCATTTA+TGG + chr4_1:82027246-82027265 Msa0577690:intron 25.0%
! CTGAGTACATAATTTATTCT+CGG + chr4_1:82027466-82027485 Msa0577690:intron 25.0%
! TGAGTACATAATTTATTCTC+GGG + chr4_1:82027467-82027486 Msa0577690:intron 25.0%
! GTATATCAAACATTTGATCA+CGG - chr4_1:82027676-82027695 None:intergenic 25.0%
!! GTATAGTCATATTTTCTACA+GGG - chr4_1:82028041-82028060 None:intergenic 25.0%
!! AGTATAGTCATATTTTCTAC+AGG - chr4_1:82028042-82028061 None:intergenic 25.0%
!! TATACTTTTCTACATGTACT+TGG + chr4_1:82028056-82028075 Msa0577690:three_prime_UTR 25.0%
! AAAGTCATTGTTTGTATTGA+TGG - chr4_1:82028260-82028279 None:intergenic 25.0%
! ATGATATTACAGATATGGAA+AGG - chr4_1:82028304-82028323 None:intergenic 25.0%
AATTAGGCATATTAGGTAAG+TGG + chr4_1:82026149-82026168 Msa0577690:five_prime_UTR 30.0%
GATTTCTGAATATCTGTGAA+CGG - chr4_1:82026226-82026245 None:intergenic 30.0%
GTAGAATCATGACAAAAACA+AGG - chr4_1:82026453-82026472 None:intergenic 30.0%
!! ATTGAGATTTGGAATTTTGG+TGG + chr4_1:82026724-82026743 Msa0577690:intron 30.0%
TCCTAATTCTTGTGATGAAT+TGG + chr4_1:82026990-82027009 Msa0577690:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG + chr4_1:82026991-82027010 Msa0577690:intron 30.0%
ACTGAACCGATAGTTAAAAT+CGG + chr4_1:82027070-82027089 Msa0577690:intron 30.0%
AATTAACAATAGTTCAGCTC+TGG - chr4_1:82027101-82027120 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG + chr4_1:82027101-82027120 Msa0577690:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG + chr4_1:82027113-82027132 Msa0577690:intron 30.0%
CGAGAATAAATTATGTACTC+AGG - chr4_1:82027468-82027487 None:intergenic 30.0%
TTAACAATTCCCTATGGAAT+TGG + chr4_1:82027646-82027665 Msa0577690:intron 30.0%
ATGTTTGATATACCTTCTCA+AGG + chr4_1:82027683-82027702 Msa0577690:intron 30.0%
TATAGCTCATGAAAATGCTA+TGG - chr4_1:82027821-82027840 None:intergenic 30.0%
ATGAAAATGCTATCGAAGAA+AGG - chr4_1:82027848-82027867 None:intergenic 30.0%
AATTAGGCATATTAGGTAAG+TGG + chr4_1:82026149-82026168 Msa0577690:five_prime_UTR 30.0%
GATTTCTGAATATCTGTGAA+CGG - chr4_1:82026226-82026245 None:intergenic 30.0%
GTAGAATCATGACAAAAACA+AGG - chr4_1:82026453-82026472 None:intergenic 30.0%
!! ATTGAGATTTGGAATTTTGG+TGG + chr4_1:82026724-82026743 Msa0577690:intron 30.0%
TCCTAATTCTTGTGATGAAT+TGG + chr4_1:82026990-82027009 Msa0577690:intron 30.0%
CCTAATTCTTGTGATGAATT+GGG + chr4_1:82026991-82027010 Msa0577690:intron 30.0%
ACTGAACCGATAGTTAAAAT+CGG + chr4_1:82027070-82027089 Msa0577690:intron 30.0%
AATTAACAATAGTTCAGCTC+TGG - chr4_1:82027101-82027120 None:intergenic 30.0%
GAGCTGAACTATTGTTAATT+CGG + chr4_1:82027101-82027120 Msa0577690:intron 30.0%
TGTTAATTCGGATATTTGAG+AGG + chr4_1:82027113-82027132 Msa0577690:intron 30.0%
CGAGAATAAATTATGTACTC+AGG - chr4_1:82027468-82027487 None:intergenic 30.0%
TTAACAATTCCCTATGGAAT+TGG + chr4_1:82027646-82027665 Msa0577690:intron 30.0%
ATGTTTGATATACCTTCTCA+AGG + chr4_1:82027683-82027702 Msa0577690:intron 30.0%
TATAGCTCATGAAAATGCTA+TGG - chr4_1:82027821-82027840 None:intergenic 30.0%
ATGAAAATGCTATCGAAGAA+AGG - chr4_1:82027848-82027867 None:intergenic 30.0%
!! ACGAACCATGATTGATTTGT+AGG - chr4_1:82026350-82026369 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG - chr4_1:82026380-82026399 None:intergenic 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG - chr4_1:82026839-82026858 None:intergenic 35.0%
CCAGAAACAACTGTTAAGAA+AGG + chr4_1:82026836-82026855 Msa0577690:CDS 35.0%
AAAGGAAATGACTATCCTGT+TGG + chr4_1:82026854-82026873 Msa0577690:CDS 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG + chr4_1:82026893-82026912 Msa0577690:CDS 35.0%
TAAGGAGGACAACATTGAAA+GGG - chr4_1:82026933-82026952 None:intergenic 35.0%
ATAAGGAGGACAACATTGAA+AGG - chr4_1:82026934-82026953 None:intergenic 35.0%
CCCAATTCATCACAAGAATT+AGG - chr4_1:82026994-82027013 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+CGG - chr4_1:82027079-82027098 None:intergenic 35.0%
!! GGTTCAATCGTTGTTTTGTT+TGG + chr4_1:82027134-82027153 Msa0577690:intron 35.0%
TCAAGCTGTTGAACTATTTG+AGG + chr4_1:82027158-82027177 Msa0577690:intron 35.0%
! TTGAACTATTTGAGGTCTTG+TGG + chr4_1:82027166-82027185 Msa0577690:intron 35.0%
GTCTTTACCCTCTGTAATAT+AGG + chr4_1:82027236-82027255 Msa0577690:intron 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG + chr4_1:82027316-82027335 Msa0577690:intron 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG - chr4_1:82027330-82027349 None:intergenic 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG - chr4_1:82027331-82027350 None:intergenic 35.0%
!! CGTTTTTGTTTGGCCTATTA+GGG - chr4_1:82027332-82027351 None:intergenic 35.0%
!! TCGTTTTTGTTTGGCCTATT+AGG - chr4_1:82027333-82027352 None:intergenic 35.0%
AATCGTATTATAGTGCAGAG+AGG + chr4_1:82027593-82027612 Msa0577690:intron 35.0%
ATCGTATTATAGTGCAGAGA+GGG + chr4_1:82027594-82027613 Msa0577690:intron 35.0%
AGGGATTTAACAATTCCCTA+TGG + chr4_1:82027640-82027659 Msa0577690:intron 35.0%
GATATACCTTCTCAAGGATT+AGG + chr4_1:82027689-82027708 Msa0577690:intron 35.0%
TATCATCCTAATCCTTGAGA+AGG - chr4_1:82027698-82027717 None:intergenic 35.0%
ATGAACATGCTATGGAAGAA+AGG - chr4_1:82027727-82027746 None:intergenic 35.0%
! ACTTTTCTACATGTACTTGG+AGG + chr4_1:82028059-82028078 Msa0577690:three_prime_UTR 35.0%
AACTTTCCCAAACTTGTGTA+TGG - chr4_1:82028120-82028139 None:intergenic 35.0%
!!! TGTGCTCTTTTTTGATCTGA+TGG - chr4_1:82028152-82028171 None:intergenic 35.0%
!! TTGTTTGTATTGATGGTGGT+GGG - chr4_1:82028253-82028272 None:intergenic 35.0%
!! ATTGTTTGTATTGATGGTGG+TGG - chr4_1:82028254-82028273 None:intergenic 35.0%
! GTCATTGTTTGTATTGATGG+TGG - chr4_1:82028257-82028276 None:intergenic 35.0%
!! ACGAACCATGATTGATTTGT+AGG - chr4_1:82026350-82026369 None:intergenic 35.0%
GAATGCAAGAAATAGAAGAG+AGG - chr4_1:82026380-82026399 None:intergenic 35.0%
! CCTTTCTTAACAGTTGTTTC+TGG - chr4_1:82026839-82026858 None:intergenic 35.0%
CCAGAAACAACTGTTAAGAA+AGG + chr4_1:82026836-82026855 Msa0577690:CDS 35.0%
AAAGGAAATGACTATCCTGT+TGG + chr4_1:82026854-82026873 Msa0577690:CDS 35.0%
! TTCTTCGTCTTTGTTGTCAT+TGG + chr4_1:82026893-82026912 Msa0577690:CDS 35.0%
TAAGGAGGACAACATTGAAA+GGG - chr4_1:82026933-82026952 None:intergenic 35.0%
ATAAGGAGGACAACATTGAA+AGG - chr4_1:82026934-82026953 None:intergenic 35.0%
CCCAATTCATCACAAGAATT+AGG - chr4_1:82026994-82027013 None:intergenic 35.0%
!!! GCTCAACCGATTTTAACTAT+CGG - chr4_1:82027079-82027098 None:intergenic 35.0%
!! GGTTCAATCGTTGTTTTGTT+TGG + chr4_1:82027134-82027153 Msa0577690:intron 35.0%
TCAAGCTGTTGAACTATTTG+AGG + chr4_1:82027158-82027177 Msa0577690:intron 35.0%
! TTGAACTATTTGAGGTCTTG+TGG + chr4_1:82027166-82027185 Msa0577690:intron 35.0%
GTCTTTACCCTCTGTAATAT+AGG + chr4_1:82027236-82027255 Msa0577690:intron 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG + chr4_1:82027316-82027335 Msa0577690:intron 35.0%
!! TTTTTGTTTGGCCTATTAGG+GGG - chr4_1:82027330-82027349 None:intergenic 35.0%
!! GTTTTTGTTTGGCCTATTAG+GGG - chr4_1:82027331-82027350 None:intergenic 35.0%
!! CGTTTTTGTTTGGCCTATTA+GGG - chr4_1:82027332-82027351 None:intergenic 35.0%
!! TCGTTTTTGTTTGGCCTATT+AGG - chr4_1:82027333-82027352 None:intergenic 35.0%
AATCGTATTATAGTGCAGAG+AGG + chr4_1:82027593-82027612 Msa0577690:intron 35.0%
ATCGTATTATAGTGCAGAGA+GGG + chr4_1:82027594-82027613 Msa0577690:intron 35.0%
AGGGATTTAACAATTCCCTA+TGG + chr4_1:82027640-82027659 Msa0577690:intron 35.0%
GATATACCTTCTCAAGGATT+AGG + chr4_1:82027689-82027708 Msa0577690:intron 35.0%
TATCATCCTAATCCTTGAGA+AGG - chr4_1:82027698-82027717 None:intergenic 35.0%
ATGAACATGCTATGGAAGAA+AGG - chr4_1:82027727-82027746 None:intergenic 35.0%
! ACTTTTCTACATGTACTTGG+AGG + chr4_1:82028059-82028078 Msa0577690:three_prime_UTR 35.0%
AACTTTCCCAAACTTGTGTA+TGG - chr4_1:82028120-82028139 None:intergenic 35.0%
!!! TGTGCTCTTTTTTGATCTGA+TGG - chr4_1:82028152-82028171 None:intergenic 35.0%
!! TTGTTTGTATTGATGGTGGT+GGG - chr4_1:82028253-82028272 None:intergenic 35.0%
!! ATTGTTTGTATTGATGGTGG+TGG - chr4_1:82028254-82028273 None:intergenic 35.0%
! GTCATTGTTTGTATTGATGG+TGG - chr4_1:82028257-82028276 None:intergenic 35.0%
! ACCACCGTCTTTCAATTTTC+CGG + chr4_1:82026290-82026309 Msa0577690:five_prime_UTR 40.0%
AACGCCGGAAAATTGAAAGA+CGG - chr4_1:82026297-82026316 None:intergenic 40.0%
!! TTTGTAGGATAATGGATCGC+CGG - chr4_1:82026335-82026354 None:intergenic 40.0%
ACGAAGAAACCAAGCAAAAC+GGG - chr4_1:82026881-82026900 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG - chr4_1:82026882-82026901 None:intergenic 40.0%
ATTATAGTGCAGAGAGGGAA+AGG + chr4_1:82027599-82027618 Msa0577690:intron 40.0%
TTATAGTGCAGAGAGGGAAA+GGG + chr4_1:82027600-82027619 Msa0577690:intron 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG + chr4_1:82027621-82027640 Msa0577690:intron 40.0%
CATAGCTCATGAACATGCTA+TGG - chr4_1:82027735-82027754 None:intergenic 40.0%
CAGCTCTCTTTCAGATAATC+AGG + chr4_1:82027951-82027970 Msa0577690:intron 40.0%
TTTCTACATGTACTTGGAGG+TGG + chr4_1:82028062-82028081 Msa0577690:three_prime_UTR 40.0%
TTGTGCACCATACACAAGTT+TGG + chr4_1:82028110-82028129 Msa0577690:three_prime_UTR 40.0%
TGTGCACCATACACAAGTTT+GGG + chr4_1:82028111-82028130 Msa0577690:three_prime_UTR 40.0%
! ACCACCGTCTTTCAATTTTC+CGG + chr4_1:82026290-82026309 Msa0577690:five_prime_UTR 40.0%
AACGCCGGAAAATTGAAAGA+CGG - chr4_1:82026297-82026316 None:intergenic 40.0%
!! TTTGTAGGATAATGGATCGC+CGG - chr4_1:82026335-82026354 None:intergenic 40.0%
ACGAAGAAACCAAGCAAAAC+GGG - chr4_1:82026881-82026900 None:intergenic 40.0%
GACGAAGAAACCAAGCAAAA+CGG - chr4_1:82026882-82026901 None:intergenic 40.0%
ATTATAGTGCAGAGAGGGAA+AGG + chr4_1:82027599-82027618 Msa0577690:intron 40.0%
TTATAGTGCAGAGAGGGAAA+GGG + chr4_1:82027600-82027619 Msa0577690:intron 40.0%
!! GGTAATGGGTAATGGTCAAA+GGG + chr4_1:82027621-82027640 Msa0577690:intron 40.0%
CATAGCTCATGAACATGCTA+TGG - chr4_1:82027735-82027754 None:intergenic 40.0%
CAGCTCTCTTTCAGATAATC+AGG + chr4_1:82027951-82027970 Msa0577690:intron 40.0%
TTTCTACATGTACTTGGAGG+TGG + chr4_1:82028062-82028081 Msa0577690:three_prime_UTR 40.0%
TTGTGCACCATACACAAGTT+TGG + chr4_1:82028110-82028129 Msa0577690:three_prime_UTR 40.0%
TGTGCACCATACACAAGTTT+GGG + chr4_1:82028111-82028130 Msa0577690:three_prime_UTR 40.0%
TGAACGGTGATCGTTGTTAC+CGG - chr4_1:82026210-82026229 None:intergenic 45.0%
!! ACGGTGGTGATGTTCTTTTC+CGG - chr4_1:82026278-82026297 None:intergenic 45.0%
TGGAAAAACGAAGAGAACGC+CGG - chr4_1:82026312-82026331 None:intergenic 45.0%
!! CGTTCTCTTCGTTTTTCCAC+CGG + chr4_1:82026313-82026332 Msa0577690:five_prime_UTR 45.0%
TCTCAACCTTCCTAACTGCA+AGG - chr4_1:82026781-82026800 None:intergenic 45.0%
CGAAGAAACCAAGCAAAACG+GGG - chr4_1:82026880-82026899 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG + chr4_1:82027613-82027632 Msa0577690:intron 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG + chr4_1:82027620-82027639 Msa0577690:intron 45.0%
ATAATCAGGACAGCAACGAG+TGG + chr4_1:82027965-82027984 Msa0577690:CDS 45.0%
!! GGCATAATGGAGCTTTGAGT+TGG + chr4_1:82027994-82028013 Msa0577690:exon 45.0%
!! TATTGATGGTGGTGGGTCAA+TGG - chr4_1:82028246-82028265 None:intergenic 45.0%
TGAACGGTGATCGTTGTTAC+CGG - chr4_1:82026210-82026229 None:intergenic 45.0%
!! ACGGTGGTGATGTTCTTTTC+CGG - chr4_1:82026278-82026297 None:intergenic 45.0%
TGGAAAAACGAAGAGAACGC+CGG - chr4_1:82026312-82026331 None:intergenic 45.0%
!! CGTTCTCTTCGTTTTTCCAC+CGG + chr4_1:82026313-82026332 Msa0577690:five_prime_UTR 45.0%
TCTCAACCTTCCTAACTGCA+AGG - chr4_1:82026781-82026800 None:intergenic 45.0%
CGAAGAAACCAAGCAAAACG+GGG - chr4_1:82026880-82026899 None:intergenic 45.0%
!! AGGGAAAGGGTAATGGGTAA+TGG + chr4_1:82027613-82027632 Msa0577690:intron 45.0%
!! GGGTAATGGGTAATGGTCAA+AGG + chr4_1:82027620-82027639 Msa0577690:intron 45.0%
ATAATCAGGACAGCAACGAG+TGG + chr4_1:82027965-82027984 Msa0577690:CDS 45.0%
!! GGCATAATGGAGCTTTGAGT+TGG + chr4_1:82027994-82028013 Msa0577690:exon 45.0%
!! TATTGATGGTGGTGGGTCAA+TGG - chr4_1:82028246-82028265 None:intergenic 45.0%
!! AAATAAATAAATAAAAAAGT+TGG + chr4_1:82026174-82026193 Msa0577690:five_prime_UTR 5.0%
!! TAAATAAAAAAAAAATTACA+GGG - chr4_1:82027278-82027297 None:intergenic 5.0%
!! AAATAAATAAATAAAAAAGT+TGG + chr4_1:82026174-82026193 Msa0577690:five_prime_UTR 5.0%
!! TAAATAAAAAAAAAATTACA+GGG - chr4_1:82027278-82027297 None:intergenic 5.0%
GCCGGAAAATTGAAAGACGG+TGG - chr4_1:82026294-82026313 None:intergenic 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG + chr4_1:82026768-82026787 Msa0577690:CDS 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG + chr4_1:82026772-82026791 Msa0577690:CDS 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG + chr4_1:82027606-82027625 Msa0577690:intron 50.0%
GCAGAGAGGGAAAGGGTAAT+GGG + chr4_1:82027607-82027626 Msa0577690:intron 50.0%
! CACGGAGCACCAATTCCATA+GGG - chr4_1:82027658-82027677 None:intergenic 50.0%
! TCACGGAGCACCAATTCCAT+AGG - chr4_1:82027659-82027678 None:intergenic 50.0%
GGCAGAGGTGGAAGATTGAA+AGG - chr4_1:82028225-82028244 None:intergenic 50.0%
GCCGGAAAATTGAAAGACGG+TGG - chr4_1:82026294-82026313 None:intergenic 50.0%
! CATCAAAGCGCCTTGCAGTT+AGG + chr4_1:82026768-82026787 Msa0577690:CDS 50.0%
! AAAGCGCCTTGCAGTTAGGA+AGG + chr4_1:82026772-82026791 Msa0577690:CDS 50.0%
TGCAGAGAGGGAAAGGGTAA+TGG + chr4_1:82027606-82027625 Msa0577690:intron 50.0%
GCAGAGAGGGAAAGGGTAAT+GGG + chr4_1:82027607-82027626 Msa0577690:intron 50.0%
! CACGGAGCACCAATTCCATA+GGG - chr4_1:82027658-82027677 None:intergenic 50.0%
! TCACGGAGCACCAATTCCAT+AGG - chr4_1:82027659-82027678 None:intergenic 50.0%
GGCAGAGGTGGAAGATTGAA+AGG - chr4_1:82028225-82028244 None:intergenic 50.0%
!! GTAGGATAATGGATCGCCGG+TGG - chr4_1:82026332-82026351 None:intergenic 55.0%
ATCAGGACAGCAACGAGTGG+AGG + chr4_1:82027968-82027987 Msa0577690:CDS 55.0%
! CGAGTGGAGGCATGGCATAA+TGG + chr4_1:82027981-82028000 Msa0577690:CDS 55.0%
!! GTAGGATAATGGATCGCCGG+TGG - chr4_1:82026332-82026351 None:intergenic 55.0%
ATCAGGACAGCAACGAGTGG+AGG + chr4_1:82027968-82027987 Msa0577690:CDS 55.0%
! CGAGTGGAGGCATGGCATAA+TGG + chr4_1:82027981-82028000 Msa0577690:CDS 55.0%
ACCTCGGAGAGAGTCACTGC+CGG + chr4_1:82026256-82026275 Msa0577690:five_prime_UTR 60.0%
CCAAGCAAAACGGGGCCAAC+AGG - chr4_1:82026872-82026891 None:intergenic 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG + chr4_1:82026869-82026888 Msa0577690:CDS 60.0%
GACAGCAACGAGTGGAGGCA+TGG + chr4_1:82027973-82027992 Msa0577690:CDS 60.0%
! TGGTGGGTCAATGGCAGAGG+TGG - chr4_1:82028237-82028256 None:intergenic 60.0%
!! TGGTGGTGGGTCAATGGCAG+AGG - chr4_1:82028240-82028259 None:intergenic 60.0%
ACCTCGGAGAGAGTCACTGC+CGG + chr4_1:82026256-82026275 Msa0577690:five_prime_UTR 60.0%
CCAAGCAAAACGGGGCCAAC+AGG - chr4_1:82026872-82026891 None:intergenic 60.0%
! CCTGTTGGCCCCGTTTTGCT+TGG + chr4_1:82026869-82026888 Msa0577690:CDS 60.0%
GACAGCAACGAGTGGAGGCA+TGG + chr4_1:82027973-82027992 Msa0577690:CDS 60.0%
! TGGTGGGTCAATGGCAGAGG+TGG - chr4_1:82028237-82028256 None:intergenic 60.0%
!! TGGTGGTGGGTCAATGGCAG+AGG - chr4_1:82028240-82028259 None:intergenic 60.0%
TCCGGCAGTGACTCTCTCCG+AGG - chr4_1:82026260-82026279 None:intergenic 65.0%
TCCGGCAGTGACTCTCTCCG+AGG - chr4_1:82026260-82026279 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 82026135 82028333 82026135 ID=Msa0577690;Name=Msa0577690
chr4_1 mRNA 82026135 82028333 82026135 ID=Msa0577690-mRNA-1;Parent=Msa0577690;Name=Msa0577690-mRNA-1;_AED=0.01;_eAED=0.01;_QI=226|0.5|0.66|1|1|1|3|322|78
chr4_1 exon 82026135 82026363 82026135 ID=Msa0577690-mRNA-1:exon:29600;Parent=Msa0577690-mRNA-1
chr4_1 exon 82026764 82026939 82026764 ID=Msa0577690-mRNA-1:exon:29601;Parent=Msa0577690-mRNA-1
chr4_1 exon 82027954 82028333 82027954 ID=Msa0577690-mRNA-1:exon:29602;Parent=Msa0577690-mRNA-1
chr4_1 five_prime_UTR 82026135 82026360 82026135 ID=Msa0577690-mRNA-1:five_prime_utr;Parent=Msa0577690-mRNA-1
chr4_1 CDS 82026361 82026363 82026361 ID=Msa0577690-mRNA-1:cds;Parent=Msa0577690-mRNA-1
chr4_1 CDS 82026764 82026939 82026764 ID=Msa0577690-mRNA-1:cds;Parent=Msa0577690-mRNA-1
chr4_1 CDS 82027954 82028011 82027954 ID=Msa0577690-mRNA-1:cds;Parent=Msa0577690-mRNA-1
chr4_1 three_prime_UTR 82028012 82028333 82028012 ID=Msa0577690-mRNA-1:three_prime_utr;Parent=Msa0577690-mRNA-1
Gene Sequence

>Msa0577690

ATGACTACATCAAAGCGCCTTGCAGTTAGGAAGGTTGAGAAGTTCGAGAAAAATATCACAAAAAGAGGAAGTGTGCCAGAAACAACTGTTAAGAAAGGAAATGACTATCCTGTTGGCCCCGTTTTGCTTGGTTTCTTCGTCTTTGTTGTCATTGGATCATGTAAGATTGCCCTTTCAATCTCTCTTTCAGATAATCAGGACAGCAACGAGTGGAGGCATGGCATAATGGAGCTTTGA
Protein sequence

>Msa0577690

MTTSKRLAVRKVEKFEKNITKRGSVPETTVKKGNDYPVGPVLLGFFVFVVIGSCKIALSISLSDNQDSNEWRHGIMEL*