AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0407870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0407870 MtrunA17_Chr3g0098291 66.452 155 50 2 14 166 1 155 2.44e-65 200
Msa0407870 MtrunA17_Chr3g0098521 70.161 124 37 0 44 167 1 124 6.93e-58 178
Msa0407870 MtrunA17_Chr3g0098491 72.881 118 32 0 44 161 1 118 1.57e-57 177
Msa0407870 MtrunA17_Chr3g0102001 42.424 132 74 2 38 167 21 152 3.65e-27 107
Msa0407870 MtrunA17_Chr5g0439021 39.683 126 68 2 41 161 25 147 1.34e-23 92.0
Msa0407870 MtrunA17_Chr3g0098541 48.039 102 52 1 67 167 5 106 1.79e-23 94.7
Msa0407870 MtrunA17_Chr5g0439071 44.248 113 59 2 41 150 25 136 2.96e-21 89.7
Msa0407870 MtrunA17_Chr5g0439061 39.583 144 75 4 41 174 25 166 1.81e-19 84.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 39 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATTAGAGTCCCAATAAAA+AGG 0.231202 3_2:+47017823 Msa0407870:CDS
TCAGGCTGAATCATAGAATT+AGG 0.302724 3_2:-47017320 None:intergenic
ATAGCAAGATTGAGATCATT+TGG 0.331887 3_2:-47017917 None:intergenic
CTGTGCTTGCACGCCATAAA+AGG 0.343353 3_2:+47017867 Msa0407870:CDS
GAATGAAAATAAGAAGAATA+AGG 0.349218 3_2:-47017233 None:intergenic
TGAAACTTAATCTTAGAGTT+AGG 0.353782 3_2:-47017281 None:intergenic
TAAGTTTCAAACTTCCGAAA+TGG 0.365986 3_2:+47017256 Msa0407870:exon
CTTAGAGTTAGGATCCATTT+CGG 0.385934 3_2:-47017270 None:intergenic
CATAGAATTAGGATCCATTT+CGG 0.411404 3_2:-47017309 None:intergenic
TAAGTTTCAACCCACCGAAA+TGG 0.417432 3_2:+47017295 Msa0407870:CDS
TAGCAAGATTGAGATCATTT+GGG 0.422742 3_2:-47017916 None:intergenic
CTTCACCTTGCAGCATATAG+AGG 0.427598 3_2:+47017747 Msa0407870:CDS
CTCTTGAAATTCTTGCTACT+AGG 0.427955 3_2:+47018185 Msa0407870:three_prime_UTR
AGTTTGTAATGGAAGACAAA+TGG 0.447631 3_2:+47017535 Msa0407870:CDS
CGTGTTCCGCGAGTTTGTAA+TGG 0.455705 3_2:+47017524 Msa0407870:CDS
AATAAGGATCAATGAGAGAT+GGG 0.462738 3_2:-47017217 None:intergenic
ACCGAACATTTCTCTATGAA+TGG 0.471652 3_2:-47017972 None:intergenic
GAATAAGGATCAATGAGAGA+TGG 0.481962 3_2:-47017218 None:intergenic
GCCATTCATAGAGAAATGTT+CGG 0.497959 3_2:+47017971 Msa0407870:three_prime_UTR
GATCAATGAGAGATGGGTAG+TGG 0.515618 3_2:-47017211 None:intergenic
GTTTGTAATGGAAGACAAAT+GGG 0.518683 3_2:+47017536 Msa0407870:CDS
ATGAATGGCAACGTGAAGAA+TGG 0.524484 3_2:-47017957 None:intergenic
GTACCATCTTGAAAGTCATA+AGG 0.546594 3_2:-47017362 None:intergenic
GTACCTTATGACTTTCAAGA+TGG 0.546774 3_2:+47017359 Msa0407870:CDS
AGTATGTGTAAATGTATCGT+TGG 0.546925 3_2:+47017777 Msa0407870:CDS
GTCGTTATTTCAAAACCACT+TGG 0.548382 3_2:-47017705 None:intergenic
CGCTCTACAGAGGACGAACA+TGG 0.548818 3_2:+47018154 Msa0407870:three_prime_UTR
CATTACAAACTCGCGGAACA+CGG 0.552796 3_2:-47017523 None:intergenic
CTATATGCTGCAAGGTGAAG+AGG 0.554034 3_2:-47017744 None:intergenic
GAATTAGGATCCATTTCGGT+GGG 0.559223 3_2:-47017305 None:intergenic
AAATTGATGTAGGTAAAGAA+AGG 0.583759 3_2:-47017891 None:intergenic
TGAATCCTCTATATGCTGCA+AGG 0.593278 3_2:-47017752 None:intergenic
CAATCTTGCTATGAATAACG+AGG 0.598611 3_2:+47017928 Msa0407870:exon
AGAATTAGGATCCATTTCGG+TGG 0.599853 3_2:-47017306 None:intergenic
CTAGTAGCAAGAATTTCAAG+AGG 0.608826 3_2:-47018184 None:intergenic
GTAAAGCATAATGATCCAAG+TGG 0.651657 3_2:+47017690 Msa0407870:CDS
TGTCTTCCATTACAAACTCG+CGG 0.659366 3_2:-47017530 None:intergenic
AAGAGGAACAGCATTACATG+TGG 0.700334 3_2:+47017617 Msa0407870:CDS
AATCTTGCTATGAATAACGA+GGG 0.709353 3_2:+47017929 Msa0407870:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATATTTTTTATCAAGTTT+TGG - chr3_2:47018041-47018060 None:intergenic 10.0%
!!! AAATATTTTTTATCAAGTTT+TGG - chr3_2:47018041-47018060 None:intergenic 10.0%
!! TTAATGTCACATTAAAAAAT+TGG - chr3_2:47017455-47017474 None:intergenic 15.0%
!!! TTTTCACAAGATAAAAATAA+AGG + chr3_2:47017593-47017612 Msa0407870:CDS 15.0%
!! TTTCACAAGATAAAAATAAA+GGG + chr3_2:47017594-47017613 Msa0407870:CDS 15.0%
!! TTAATGTCACATTAAAAAAT+TGG - chr3_2:47017455-47017474 None:intergenic 15.0%
!!! TTTTCACAAGATAAAAATAA+AGG + chr3_2:47017593-47017612 Msa0407870:CDS 15.0%
!! TTTCACAAGATAAAAATAAA+GGG + chr3_2:47017594-47017613 Msa0407870:CDS 15.0%
!! GAATGAAAATAAGAAGAATA+AGG - chr3_2:47017236-47017255 None:intergenic 20.0%
!! GAATGAAAATAAGAAGAATA+AGG - chr3_2:47017236-47017255 None:intergenic 20.0%
! TGAAACTTAATCTTAGAGTT+AGG - chr3_2:47017284-47017303 None:intergenic 25.0%
! AAACTCTCCTTATATTTATC+AGG + chr3_2:47017389-47017408 Msa0407870:intron 25.0%
! ATGTCACATTAAAAAATTGG+AGG - chr3_2:47017452-47017471 None:intergenic 25.0%
! AAGATAAAAATAAAGGGAAG+AGG + chr3_2:47017600-47017619 Msa0407870:CDS 25.0%
! AAATGTATCGTTGGAAAAAA+TGG + chr3_2:47017786-47017805 Msa0407870:CDS 25.0%
! AAATTGATGTAGGTAAAGAA+AGG - chr3_2:47017894-47017913 None:intergenic 25.0%
! TGAAACTTAATCTTAGAGTT+AGG - chr3_2:47017284-47017303 None:intergenic 25.0%
! AAACTCTCCTTATATTTATC+AGG + chr3_2:47017389-47017408 Msa0407870:intron 25.0%
! ATGTCACATTAAAAAATTGG+AGG - chr3_2:47017452-47017471 None:intergenic 25.0%
! AAGATAAAAATAAAGGGAAG+AGG + chr3_2:47017600-47017619 Msa0407870:CDS 25.0%
! AAATGTATCGTTGGAAAAAA+TGG + chr3_2:47017786-47017805 Msa0407870:CDS 25.0%
! AAATTGATGTAGGTAAAGAA+AGG - chr3_2:47017894-47017913 None:intergenic 25.0%
AATAAGGATCAATGAGAGAT+GGG - chr3_2:47017220-47017239 None:intergenic 30.0%
TAAGTTTCAAACTTCCGAAA+TGG + chr3_2:47017256-47017275 Msa0407870:exon 30.0%
CATAGAATTAGGATCCATTT+CGG - chr3_2:47017312-47017331 None:intergenic 30.0%
! ACATTTTCATCTTCATCTTC+AGG - chr3_2:47017341-47017360 None:intergenic 30.0%
TGAAGAGCCTGATAAATATA+AGG - chr3_2:47017399-47017418 None:intergenic 30.0%
! AGTTTGTAATGGAAGACAAA+TGG + chr3_2:47017535-47017554 Msa0407870:CDS 30.0%
! GTTTGTAATGGAAGACAAAT+GGG + chr3_2:47017536-47017555 Msa0407870:CDS 30.0%
AGTATGTGTAAATGTATCGT+TGG + chr3_2:47017777-47017796 Msa0407870:CDS 30.0%
TGATTAGAGTCCCAATAAAA+AGG + chr3_2:47017823-47017842 Msa0407870:CDS 30.0%
GATTAGAGTCCCAATAAAAA+GGG + chr3_2:47017824-47017843 Msa0407870:CDS 30.0%
CATTTGGGAAAAATTGATGT+AGG - chr3_2:47017904-47017923 None:intergenic 30.0%
TAGCAAGATTGAGATCATTT+GGG - chr3_2:47017919-47017938 None:intergenic 30.0%
ATAGCAAGATTGAGATCATT+TGG - chr3_2:47017920-47017939 None:intergenic 30.0%
! AATCTTGCTATGAATAACGA+GGG + chr3_2:47017929-47017948 Msa0407870:exon 30.0%
AATAAGGATCAATGAGAGAT+GGG - chr3_2:47017220-47017239 None:intergenic 30.0%
TAAGTTTCAAACTTCCGAAA+TGG + chr3_2:47017256-47017275 Msa0407870:exon 30.0%
CATAGAATTAGGATCCATTT+CGG - chr3_2:47017312-47017331 None:intergenic 30.0%
! ACATTTTCATCTTCATCTTC+AGG - chr3_2:47017341-47017360 None:intergenic 30.0%
TGAAGAGCCTGATAAATATA+AGG - chr3_2:47017399-47017418 None:intergenic 30.0%
! AGTTTGTAATGGAAGACAAA+TGG + chr3_2:47017535-47017554 Msa0407870:CDS 30.0%
! GTTTGTAATGGAAGACAAAT+GGG + chr3_2:47017536-47017555 Msa0407870:CDS 30.0%
AGTATGTGTAAATGTATCGT+TGG + chr3_2:47017777-47017796 Msa0407870:CDS 30.0%
TGATTAGAGTCCCAATAAAA+AGG + chr3_2:47017823-47017842 Msa0407870:CDS 30.0%
GATTAGAGTCCCAATAAAAA+GGG + chr3_2:47017824-47017843 Msa0407870:CDS 30.0%
CATTTGGGAAAAATTGATGT+AGG - chr3_2:47017904-47017923 None:intergenic 30.0%
TAGCAAGATTGAGATCATTT+GGG - chr3_2:47017919-47017938 None:intergenic 30.0%
ATAGCAAGATTGAGATCATT+TGG - chr3_2:47017920-47017939 None:intergenic 30.0%
! AATCTTGCTATGAATAACGA+GGG + chr3_2:47017929-47017948 Msa0407870:exon 30.0%
GAATAAGGATCAATGAGAGA+TGG - chr3_2:47017221-47017240 None:intergenic 35.0%
CTTAGAGTTAGGATCCATTT+CGG - chr3_2:47017273-47017292 None:intergenic 35.0%
TCAGGCTGAATCATAGAATT+AGG - chr3_2:47017323-47017342 None:intergenic 35.0%
GTACCTTATGACTTTCAAGA+TGG + chr3_2:47017359-47017378 Msa0407870:CDS 35.0%
GTACCATCTTGAAAGTCATA+AGG - chr3_2:47017365-47017384 None:intergenic 35.0%
! AAGTTTTACTCTCTCTTCGT+CGG - chr3_2:47017655-47017674 None:intergenic 35.0%
GTAAAGCATAATGATCCAAG+TGG + chr3_2:47017690-47017709 Msa0407870:CDS 35.0%
! GTCGTTATTTCAAAACCACT+TGG - chr3_2:47017708-47017727 None:intergenic 35.0%
TCGTTGGAAAAAATGGTGAA+AGG + chr3_2:47017793-47017812 Msa0407870:CDS 35.0%
! GGTGTTTCTCCCTTTTTATT+GGG - chr3_2:47017836-47017855 None:intergenic 35.0%
! GGTAAAGAAAGGTCCTTTTA+TGG - chr3_2:47017883-47017902 None:intergenic 35.0%
! CAATCTTGCTATGAATAACG+AGG + chr3_2:47017928-47017947 Msa0407870:exon 35.0%
GCCATTCATAGAGAAATGTT+CGG + chr3_2:47017971-47017990 Msa0407870:three_prime_UTR 35.0%
ACCGAACATTTCTCTATGAA+TGG - chr3_2:47017975-47017994 None:intergenic 35.0%
ATCTATGTGTCTTATGCAAC+AGG + chr3_2:47018094-47018113 Msa0407870:intron 35.0%
CTAGTAGCAAGAATTTCAAG+AGG - chr3_2:47018187-47018206 None:intergenic 35.0%
CTCTTGAAATTCTTGCTACT+AGG + chr3_2:47018185-47018204 Msa0407870:three_prime_UTR 35.0%
GAATAAGGATCAATGAGAGA+TGG - chr3_2:47017221-47017240 None:intergenic 35.0%
CTTAGAGTTAGGATCCATTT+CGG - chr3_2:47017273-47017292 None:intergenic 35.0%
TCAGGCTGAATCATAGAATT+AGG - chr3_2:47017323-47017342 None:intergenic 35.0%
GTACCTTATGACTTTCAAGA+TGG + chr3_2:47017359-47017378 Msa0407870:CDS 35.0%
GTACCATCTTGAAAGTCATA+AGG - chr3_2:47017365-47017384 None:intergenic 35.0%
! AAGTTTTACTCTCTCTTCGT+CGG - chr3_2:47017655-47017674 None:intergenic 35.0%
GTAAAGCATAATGATCCAAG+TGG + chr3_2:47017690-47017709 Msa0407870:CDS 35.0%
! GTCGTTATTTCAAAACCACT+TGG - chr3_2:47017708-47017727 None:intergenic 35.0%
TCGTTGGAAAAAATGGTGAA+AGG + chr3_2:47017793-47017812 Msa0407870:CDS 35.0%
! GGTGTTTCTCCCTTTTTATT+GGG - chr3_2:47017836-47017855 None:intergenic 35.0%
! GGTAAAGAAAGGTCCTTTTA+TGG - chr3_2:47017883-47017902 None:intergenic 35.0%
! CAATCTTGCTATGAATAACG+AGG + chr3_2:47017928-47017947 Msa0407870:exon 35.0%
GCCATTCATAGAGAAATGTT+CGG + chr3_2:47017971-47017990 Msa0407870:three_prime_UTR 35.0%
ACCGAACATTTCTCTATGAA+TGG - chr3_2:47017975-47017994 None:intergenic 35.0%
ATCTATGTGTCTTATGCAAC+AGG + chr3_2:47018094-47018113 Msa0407870:intron 35.0%
CTAGTAGCAAGAATTTCAAG+AGG - chr3_2:47018187-47018206 None:intergenic 35.0%
CTCTTGAAATTCTTGCTACT+AGG + chr3_2:47018185-47018204 Msa0407870:three_prime_UTR 35.0%
TAAGTTTCAACCCACCGAAA+TGG + chr3_2:47017295-47017314 Msa0407870:CDS 40.0%
GAATTAGGATCCATTTCGGT+GGG - chr3_2:47017308-47017327 None:intergenic 40.0%
AGAATTAGGATCCATTTCGG+TGG - chr3_2:47017309-47017328 None:intergenic 40.0%
TGTCTTCCATTACAAACTCG+CGG - chr3_2:47017533-47017552 None:intergenic 40.0%
AAGAGGAACAGCATTACATG+TGG + chr3_2:47017617-47017636 Msa0407870:CDS 40.0%
TGAATCCTCTATATGCTGCA+AGG - chr3_2:47017755-47017774 None:intergenic 40.0%
! GGGTGTTTCTCCCTTTTTAT+TGG - chr3_2:47017837-47017856 None:intergenic 40.0%
TGCAAGCACAGCACAAAAAA+GGG - chr3_2:47017858-47017877 None:intergenic 40.0%
ATGAATGGCAACGTGAAGAA+TGG - chr3_2:47017960-47017979 None:intergenic 40.0%
TGTGTCTTATGCAACAGGTA+TGG + chr3_2:47018099-47018118 Msa0407870:intron 40.0%
! TCCAATTTTTCGCTCTACAG+AGG + chr3_2:47018144-47018163 Msa0407870:three_prime_UTR 40.0%
TCCTCTGTAGAGCGAAAAAT+TGG - chr3_2:47018148-47018167 None:intergenic 40.0%
TAAGTTTCAACCCACCGAAA+TGG + chr3_2:47017295-47017314 Msa0407870:CDS 40.0%
GAATTAGGATCCATTTCGGT+GGG - chr3_2:47017308-47017327 None:intergenic 40.0%
AGAATTAGGATCCATTTCGG+TGG - chr3_2:47017309-47017328 None:intergenic 40.0%
TGTCTTCCATTACAAACTCG+CGG - chr3_2:47017533-47017552 None:intergenic 40.0%
AAGAGGAACAGCATTACATG+TGG + chr3_2:47017617-47017636 Msa0407870:CDS 40.0%
TGAATCCTCTATATGCTGCA+AGG - chr3_2:47017755-47017774 None:intergenic 40.0%
! GGGTGTTTCTCCCTTTTTAT+TGG - chr3_2:47017837-47017856 None:intergenic 40.0%
TGCAAGCACAGCACAAAAAA+GGG - chr3_2:47017858-47017877 None:intergenic 40.0%
ATGAATGGCAACGTGAAGAA+TGG - chr3_2:47017960-47017979 None:intergenic 40.0%
TGTGTCTTATGCAACAGGTA+TGG + chr3_2:47018099-47018118 Msa0407870:intron 40.0%
! TCCAATTTTTCGCTCTACAG+AGG + chr3_2:47018144-47018163 Msa0407870:three_prime_UTR 40.0%
TCCTCTGTAGAGCGAAAAAT+TGG - chr3_2:47018148-47018167 None:intergenic 40.0%
GATCAATGAGAGATGGGTAG+TGG - chr3_2:47017214-47017233 None:intergenic 45.0%
CATTACAAACTCGCGGAACA+CGG - chr3_2:47017526-47017545 None:intergenic 45.0%
CTATATGCTGCAAGGTGAAG+AGG - chr3_2:47017747-47017766 None:intergenic 45.0%
CTTCACCTTGCAGCATATAG+AGG + chr3_2:47017747-47017766 Msa0407870:CDS 45.0%
GCAAGCACAGCACAAAAAAG+GGG - chr3_2:47017857-47017876 None:intergenic 45.0%
GTGCAAGCACAGCACAAAAA+AGG - chr3_2:47017859-47017878 None:intergenic 45.0%
GATCAATGAGAGATGGGTAG+TGG - chr3_2:47017214-47017233 None:intergenic 45.0%
CATTACAAACTCGCGGAACA+CGG - chr3_2:47017526-47017545 None:intergenic 45.0%
CTATATGCTGCAAGGTGAAG+AGG - chr3_2:47017747-47017766 None:intergenic 45.0%
CTTCACCTTGCAGCATATAG+AGG + chr3_2:47017747-47017766 Msa0407870:CDS 45.0%
GCAAGCACAGCACAAAAAAG+GGG - chr3_2:47017857-47017876 None:intergenic 45.0%
GTGCAAGCACAGCACAAAAA+AGG - chr3_2:47017859-47017878 None:intergenic 45.0%
CGTGTTCCGCGAGTTTGTAA+TGG + chr3_2:47017524-47017543 Msa0407870:CDS 50.0%
CTGTGCTTGCACGCCATAAA+AGG + chr3_2:47017867-47017886 Msa0407870:CDS 50.0%
CGTGTTCCGCGAGTTTGTAA+TGG + chr3_2:47017524-47017543 Msa0407870:CDS 50.0%
CTGTGCTTGCACGCCATAAA+AGG + chr3_2:47017867-47017886 Msa0407870:CDS 50.0%
CGCTCTACAGAGGACGAACA+TGG + chr3_2:47018154-47018173 Msa0407870:three_prime_UTR 55.0%
CGCTCTACAGAGGACGAACA+TGG + chr3_2:47018154-47018173 Msa0407870:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 47017211 47018244 47017211 ID=Msa0407870;Name=Msa0407870
chr3_2 mRNA 47017211 47018244 47017211 ID=Msa0407870-mRNA-1;Parent=Msa0407870;Name=Msa0407870-mRNA-1;_AED=0.09;_eAED=0.09;_QI=64|1|1|1|0.5|0.33|3|180|175
chr3_2 exon 47017211 47017380 47017211 ID=Msa0407870-mRNA-1:exon:9043;Parent=Msa0407870-mRNA-1
chr3_2 exon 47017520 47017992 47017520 ID=Msa0407870-mRNA-1:exon:9044;Parent=Msa0407870-mRNA-1
chr3_2 exon 47018116 47018244 47018116 ID=Msa0407870-mRNA-1:exon:9045;Parent=Msa0407870-mRNA-1
chr3_2 five_prime_UTR 47017211 47017274 47017211 ID=Msa0407870-mRNA-1:five_prime_utr;Parent=Msa0407870-mRNA-1
chr3_2 CDS 47017275 47017380 47017275 ID=Msa0407870-mRNA-1:cds;Parent=Msa0407870-mRNA-1
chr3_2 CDS 47017520 47017941 47017520 ID=Msa0407870-mRNA-1:cds;Parent=Msa0407870-mRNA-1
chr3_2 three_prime_UTR 47017942 47017992 47017942 ID=Msa0407870-mRNA-1:three_prime_utr;Parent=Msa0407870-mRNA-1
chr3_2 three_prime_UTR 47018116 47018244 47018116 ID=Msa0407870-mRNA-1:three_prime_utr;Parent=Msa0407870-mRNA-1
Gene Sequence

>Msa0407870

ATGGATCCTAACTCTAAGATTAAGTTTCAACCCACCGAAATGGATCCTAATTCTATGATTCAGCCTGAAGATGAAGATGAAAATGTACCTTATGACTTTCAAGATGCTGCCGTGTTCCGCGAGTTTGTAATGGAAGACAAATGGGATGAAGTGATAGAAAAATACGAAGAACATGTATTTTTTCACAAGATAAAAATAAAGGGAAGAGGAACAGCATTACATGTGGCAGTGAGTAATGCCGACGAAGAGAGAGTAAAACTTCTTGTAGACGCAATAGTAAAGCATAATGATCCAAGTGGTTTTGAAATAACGACTGAAAGAGATGATACTCCTCTTCACCTTGCAGCATATAGAGGATTCAAAAGTATGTGTAAATGTATCGTTGGAAAAAATGGTGAAAGGAAGCATTTGATTAGAGTCCCAATAAAAAGGGAGAAACACCCCTTTTTTGTGCTGTGCTTGCACGCCATAAAAGGACCTTTCTTTACCTACATCAATTTTTCCCAAATGATCTCAATCTTGCTATGA

Protein sequence

>Msa0407870

MDPNSKIKFQPTEMDPNSMIQPEDEDENVPYDFQDAAVFREFVMEDKWDEVIEKYEEHVFFHKIKIKGRGTALHVAVSNADEERVKLLVDAIVKHNDPSGFEITTERDDTPLHLAAYRGFKSMCKCIVGKNGERKHLIRVPIKREKHPFFVLCLHAIKGPFFTYINFSQMISILL*