Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0412390 | RCV35100.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 130 | 232 | 3.26e-67 | 211 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0412390 | sp|P0CG89|H4_SOYBN | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.06e-67 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0412390 | A0A7C8Z1D6 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 1.17e-64 | 199 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0223080 | Msa0412390 | 0.814680 | 1.361543e-51 | -8.615850e-47 |
| Msa0412270 | Msa0412390 | 0.835713 | 1.429832e-56 | -8.615850e-47 |
| Msa0412390 | Msa1078170 | 0.812811 | 3.513717e-51 | -8.615850e-47 |
| Msa0412390 | Msa1093840 | 0.802683 | 4.995012e-49 | -8.615850e-47 |
| Msa0412390 | Msa1440880 | 0.853267 | 2.675332e-61 | -8.615850e-47 |
| Msa0412390 | Msa1446810 | 0.836789 | 7.614465e-57 | -8.615850e-47 |
| Msa0412390 | Msa1446820 | 0.810748 | 9.877085e-51 | -8.615850e-47 |
| Msa0412390 | Msa1451160 | 0.822753 | 2.000658e-53 | -8.615850e-47 |
| Msa0302160 | Msa0412390 | 0.813704 | 2.237200e-51 | -8.615850e-47 |
| Msa0339490 | Msa0412390 | 0.812515 | 4.077504e-51 | -8.615850e-47 |
| Msa0339500 | Msa0412390 | 0.813146 | 2.967015e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0412390 | MtrunA17_Chr4g0072411 | 100.000 | 103 | 0 | 0 | 1 | 103 | 59 | 161 | 5.39e-69 | 203 |
| Msa0412390 | MtrunA17_Chr5g0425951 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr4g0030431 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0100121 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0113681 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr2g0327641 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0103351 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr8g0352791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0103821 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0104031 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr7g0225731 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0103801 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr3g0103881 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Msa0412390 | MtrunA17_Chr7g0262791 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 2.26e-68 | 199 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0412390 | AT3G53730.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT1G07820.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT1G07820.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT5G59690.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT3G46320.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT2G28740.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT1G07660.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT3G45930.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT5G59970.1 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT5G59970.2 | 100.000 | 103 | 0 | 0 | 1 | 103 | 1 | 103 | 3.11e-68 | 199 |
| Msa0412390 | AT1G07660.2 | 83.495 | 103 | 0 | 1 | 1 | 103 | 1 | 86 | 4.52e-51 | 155 |
Find 37 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTGTCTTGAAGATCTTCT+TGG | 0.405705 | 3_2:+55822134 | Msa0412390:CDS |
| GGGAGGTAAGGGACTAGGAA+AGG | 0.428277 | 3_2:+55821984 | Msa0412390:CDS |
| GGAAGAACCCTCTATGGTTT+CGG | 0.433408 | 3_2:+55822249 | Msa0412390:CDS |
| AGGCAGGGAAGAACCCTCTA+TGG | 0.447332 | 3_2:+55822243 | Msa0412390:CDS |
| GGAGGTAAGGGACTAGGAAA+GGG | 0.452874 | 3_2:+55821985 | Msa0412390:CDS |
| ATCAACCTCCGAAACCATAG+AGG | 0.481091 | 3_2:-55822257 | None:intergenic |
| CTTGCCAATCGACGAATAGC+AGG | 0.484591 | 3_2:-55822063 | None:intergenic |
| TGTCTATGCTTTGAAGAGGC+AGG | 0.485721 | 3_2:+55822227 | Msa0412390:CDS |
| AGGTCGTGGAAAGGGAGGTA+AGG | 0.492819 | 3_2:+55821972 | Msa0412390:CDS |
| AGGGAGGTGCAAAGAGGCAT+AGG | 0.498985 | 3_2:+55822004 | Msa0412390:CDS |
| GGTTCTTCGTGACAACATCC+AGG | 0.501637 | 3_2:+55822029 | Msa0412390:CDS |
| GAAGATCTTCAAGACACCTC+TGG | 0.510902 | 3_2:-55822130 | None:intergenic |
| CTCGCGAAAATGTCAGGTCG+TGG | 0.519770 | 3_2:+55821958 | None:intergenic |
| TTACATACACTGAGCATGCT+AGG | 0.520723 | 3_2:+55822178 | Msa0412390:CDS |
| GGAAAGGGAGGTAAGGGACT+AGG | 0.521051 | 3_2:+55821979 | Msa0412390:CDS |
| CAATCGACGAATAGCAGGCT+TGG | 0.531595 | 3_2:-55822058 | None:intergenic |
| GTCTATGCTTTGAAGAGGCA+GGG | 0.532756 | 3_2:+55822228 | Msa0412390:CDS |
| CAAGCCTGCTATTCGTCGAT+TGG | 0.532828 | 3_2:+55822059 | Msa0412390:CDS |
| AAAATGTCAGGTCGTGGAAA+GGG | 0.539338 | 3_2:+55821964 | None:intergenic |
| GAAAATGTCAGGTCGTGGAA+AGG | 0.540836 | 3_2:+55821963 | None:intergenic |
| GGTGGTGTTAAGAGAATCAG+TGG | 0.556350 | 3_2:+55822090 | Msa0412390:CDS |
| GGTAAGGGACTAGGAAAGGG+AGG | 0.563878 | 3_2:+55821988 | Msa0412390:CDS |
| GGTCGTGGAAAGGGAGGTAA+GGG | 0.564245 | 3_2:+55821973 | Msa0412390:CDS |
| GAGGAAGACTGTGACAGCCA+TGG | 0.570644 | 3_2:+55822200 | Msa0412390:CDS |
| AGGTGCAAAGAGGCATAGGA+AGG | 0.586086 | 3_2:+55822008 | Msa0412390:CDS |
| CGTCGATTGGCAAGAAGAGG+TGG | 0.593946 | 3_2:+55822072 | Msa0412390:CDS |
| TAGCAGGCTTGGTGATACCC+TGG | 0.594313 | 3_2:-55822047 | None:intergenic |
| AGAACCCTCTATGGTTTCGG+AGG | 0.628880 | 3_2:+55822252 | Msa0412390:CDS |
| ATTCGTCGATTGGCAAGAAG+AGG | 0.629429 | 3_2:+55822069 | Msa0412390:CDS |
| CAAAGCATAGACAACATCCA+TGG | 0.629842 | 3_2:-55822217 | None:intergenic |
| ATGTCAGGTCGTGGAAAGGG+AGG | 0.643397 | 3_2:+55821967 | Msa0412390:CDS |
| TCAACCTCCGAAACCATAGA+GGG | 0.666233 | 3_2:-55822256 | None:intergenic |
| GTTCTTCGTGACAACATCCA+GGG | 0.676380 | 3_2:+55822030 | Msa0412390:CDS |
| TAGGAAAGGGAGGTGCAAAG+AGG | 0.683932 | 3_2:+55821998 | Msa0412390:CDS |
| ATGTTGTCTATGCTTTGAAG+AGG | 0.684273 | 3_2:+55822223 | Msa0412390:CDS |
| TTGATCTATGAAGAAACCAG+AGG | 0.704889 | 3_2:+55822114 | Msa0412390:CDS |
| CATACACTGAGCATGCTAGG+AGG | 0.707859 | 3_2:+55822181 | Msa0412390:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTGATCTATGAAGAAACCAG+AGG | + | chr3_2:55822114-55822133 | Msa0412390:CDS | 35.0% | |
| !! | ATGTTGTCTATGCTTTGAAG+AGG | + | chr3_2:55822223-55822242 | Msa0412390:CDS | 35.0% |
| AGGTGTCTTGAAGATCTTCT+TGG | + | chr3_2:55822134-55822153 | Msa0412390:CDS | 40.0% | |
| TTACATACACTGAGCATGCT+AGG | + | chr3_2:55822178-55822197 | Msa0412390:CDS | 40.0% | |
| CAAAGCATAGACAACATCCA+TGG | - | chr3_2:55822220-55822239 | None:intergenic | 40.0% | |
| GTTCTTCGTGACAACATCCA+GGG | + | chr3_2:55822030-55822049 | Msa0412390:CDS | 45.0% | |
| ! | ATTCGTCGATTGGCAAGAAG+AGG | + | chr3_2:55822069-55822088 | Msa0412390:CDS | 45.0% |
| ! | GGTGGTGTTAAGAGAATCAG+TGG | + | chr3_2:55822090-55822109 | Msa0412390:CDS | 45.0% |
| GAAGATCTTCAAGACACCTC+TGG | - | chr3_2:55822133-55822152 | None:intergenic | 45.0% | |
| !! | TGTCTATGCTTTGAAGAGGC+AGG | + | chr3_2:55822227-55822246 | Msa0412390:CDS | 45.0% |
| !! | GTCTATGCTTTGAAGAGGCA+GGG | + | chr3_2:55822228-55822247 | Msa0412390:CDS | 45.0% |
| GGAAGAACCCTCTATGGTTT+CGG | + | chr3_2:55822249-55822268 | Msa0412390:CDS | 45.0% | |
| TCAACCTCCGAAACCATAGA+GGG | - | chr3_2:55822259-55822278 | None:intergenic | 45.0% | |
| GGAGGTAAGGGACTAGGAAA+GGG | + | chr3_2:55821985-55822004 | Msa0412390:CDS | 50.0% | |
| ! | TAGGAAAGGGAGGTGCAAAG+AGG | + | chr3_2:55821998-55822017 | Msa0412390:CDS | 50.0% |
| ! | AGGTGCAAAGAGGCATAGGA+AGG | + | chr3_2:55822008-55822027 | Msa0412390:CDS | 50.0% |
| GGTTCTTCGTGACAACATCC+AGG | + | chr3_2:55822029-55822048 | Msa0412390:CDS | 50.0% | |
| CAATCGACGAATAGCAGGCT+TGG | - | chr3_2:55822061-55822080 | None:intergenic | 50.0% | |
| CAAGCCTGCTATTCGTCGAT+TGG | + | chr3_2:55822059-55822078 | Msa0412390:CDS | 50.0% | |
| CTTGCCAATCGACGAATAGC+AGG | - | chr3_2:55822066-55822085 | None:intergenic | 50.0% | |
| CATACACTGAGCATGCTAGG+AGG | + | chr3_2:55822181-55822200 | Msa0412390:CDS | 50.0% | |
| AGAACCCTCTATGGTTTCGG+AGG | + | chr3_2:55822252-55822271 | Msa0412390:CDS | 50.0% | |
| ATGTCAGGTCGTGGAAAGGG+AGG | + | chr3_2:55821967-55821986 | Msa0412390:CDS | 55.0% | |
| AGGTCGTGGAAAGGGAGGTA+AGG | + | chr3_2:55821972-55821991 | Msa0412390:CDS | 55.0% | |
| GGTCGTGGAAAGGGAGGTAA+GGG | + | chr3_2:55821973-55821992 | Msa0412390:CDS | 55.0% | |
| GGAAAGGGAGGTAAGGGACT+AGG | + | chr3_2:55821979-55821998 | Msa0412390:CDS | 55.0% | |
| GGGAGGTAAGGGACTAGGAA+AGG | + | chr3_2:55821984-55822003 | Msa0412390:CDS | 55.0% | |
| GGTAAGGGACTAGGAAAGGG+AGG | + | chr3_2:55821988-55822007 | Msa0412390:CDS | 55.0% | |
| ! | AGGGAGGTGCAAAGAGGCAT+AGG | + | chr3_2:55822004-55822023 | Msa0412390:CDS | 55.0% |
| !! | TAGCAGGCTTGGTGATACCC+TGG | - | chr3_2:55822050-55822069 | None:intergenic | 55.0% |
| ! | CGTCGATTGGCAAGAAGAGG+TGG | + | chr3_2:55822072-55822091 | Msa0412390:CDS | 55.0% |
| GAGGAAGACTGTGACAGCCA+TGG | + | chr3_2:55822200-55822219 | Msa0412390:CDS | 55.0% | |
| AGGCAGGGAAGAACCCTCTA+TGG | + | chr3_2:55822243-55822262 | Msa0412390:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_2 | gene | 55821967 | 55822278 | 55821967 | ID=Msa0412390;Name=Msa0412390 |
| chr3_2 | mRNA | 55821967 | 55822278 | 55821967 | ID=Msa0412390-mRNA-1;Parent=Msa0412390;Name=Msa0412390-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|103 |
| chr3_2 | exon | 55821967 | 55822278 | 55821967 | ID=Msa0412390-mRNA-1:exon:11360;Parent=Msa0412390-mRNA-1 |
| chr3_2 | CDS | 55821967 | 55822278 | 55821967 | ID=Msa0412390-mRNA-1:cds;Parent=Msa0412390-mRNA-1 |
| Gene Sequence |
| Protein sequence |
