AlfalfaGEDB | Msa0416660

M. sativa cultivar zhongmu-4 / Msa0416660

KEGG: K05857
GO: -
PFAM: PF00388.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0416660	XP_003600894.1	91.599	619	26	1	1	619	1	593	0.0	1140

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0416660	sp\|Q8GV43\|PLCD6_ARATH	49.762	629	260	11	7	614	20	613	0.0	600

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0416660	G7JB93	91.599	619	26	1	1	619	1	593	0.0	1140

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0217700	Msa0416660	0.801241	9.881470e-49	-8.615850e-47
Msa0369230	Msa0416660	0.865559	5.410677e-65	-8.615850e-47
Msa0416660	Msa0463270	0.855004	8.434379e-62	-8.615850e-47
Msa0416660	Msa0506860	0.812449	4.215920e-51	-8.615850e-47
Msa0416660	Msa0508880	0.836748	7.801673e-57	-8.615850e-47
Msa0416660	Msa0607320	0.806821	6.826912e-50	-8.615850e-47
Msa0416660	Msa0899970	0.816949	4.246880e-52	-8.615850e-47
Msa0416660	Msa0965080	0.812834	3.472484e-51	-8.615850e-47
Msa0416660	Msa0965290	0.815186	1.051754e-51	-8.615850e-47
Msa0416660	Msa0999680	0.819803	9.585981e-53	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0416660	MtrunA17_Chr3g0113471	91.599	619	26	1	1	619	1	593	0.0	1140
Msa0416660	MtrunA17_Chr3g0113521	81.774	620	84	3	1	619	1	592	0.0	1027
Msa0416660	MtrunA17_Chr5g0437041	55.040	625	235	10	7	614	12	607	0.0	669
Msa0416660	MtrunA17_Chr5g0430181	50.249	603	252	10	24	611	22	591	0.0	592
Msa0416660	MtrunA17_Chr5g0437061	57.905	525	163	8	113	613	1	491	0.0	589
Msa0416660	MtrunA17_Chr5g0437081	48.714	622	272	10	1	611	1	586	0.0	577
Msa0416660	MtrunA17_Chr3g0113531	47.826	621	281	9	2	613	6	592	0.0	554
Msa0416660	MtrunA17_Chr3g0112551	48.777	613	260	9	12	613	2	571	0.0	551
Msa0416660	MtrunA17_Chr5g0430191	44.137	614	278	14	3	611	17	570	9.54e-166	485
Msa0416660	MtrunA17_Chr5g0437051	42.857	91	48	1	23	109	18	108	1.94e-17	79.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0416660	AT2G40116.1	49.762	629	260	11	7	614	20	613	0.0	600
Msa0416660	AT3G08510.2	48.534	614	273	11	1	609	1	576	0.0	562
Msa0416660	AT3G08510.1	48.534	614	273	11	1	609	1	576	0.0	562
Msa0416660	AT5G58690.3	48.173	602	272	6	13	613	15	577	0.0	561
Msa0416660	AT5G58690.1	48.173	602	272	6	13	613	15	577	0.0	561
Msa0416660	AT5G58690.2	48.409	597	268	6	18	613	1	558	0.0	560
Msa0416660	AT5G58700.3	47.403	616	285	7	7	613	11	596	0.0	557
Msa0416660	AT5G58700.1	47.403	616	285	7	7	613	11	596	0.0	557
Msa0416660	AT3G55940.1	47.106	622	273	12	1	609	1	579	0.0	550
Msa0416660	AT5G58700.2	50.973	514	214	6	109	613	4	488	1.56e-176	510

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Find 133 sgRNAs with CRISPR-Local

Find 302 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGAAGGTACTATGA+AGG	0.562601	3_2:+63564664	Msa0416660:CDS
AAACAGGTCAAAGTATACAA+AGG	0.637507	3_2:+63565615	Msa0416660:intron
AAACATTTGGTTAGTGCAAC+TGG	0.337432	3_2:-63563839	None:intergenic
AAAGCTTCCTTAAGATCTAA+AGG	0.481386	3_2:-63561680	None:intergenic
AAATCACTCCGATTGATGCA+AGG	0.570110	3_2:+63565398	Msa0416660:CDS
AAATCTGCTTCTGAATCTCA+TGG	0.515731	3_2:+63564746	Msa0416660:CDS
AAATTGATTTCCAGTAAGGT+AGG	0.573386	3_2:-63563583	None:intergenic
AAATTGCACCTTATTGGATT+TGG	0.270742	3_2:+63561781	Msa0416660:CDS
AAGGAGCATCCTCAAACTGA+TGG	0.641373	3_2:+63564360	Msa0416660:CDS
AAGGATAGACTAAAAGTTGA+CGG	0.536336	3_2:+63564683	Msa0416660:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATATTTATGCATGAGATCG+AGG	-	chr3_2:63561198-63561217	None:intergenic	30.0%
TATAAACTCTCTTCCACAAA+AGG	+	chr3_2:63561341-63561360	Msa0416660:intron	30.0%
ATAAACTCTCTTCCACAAAA+GGG	+	chr3_2:63561342-63561361	Msa0416660:intron	30.0%
TATAAACTCTCTTCCACAAA+AGG	+	chr3_2:63561341-63561360	Msa0416660:intron	30.0%
ATAAACTCTCTTCCACAAAA+GGG	+	chr3_2:63561342-63561361	Msa0416660:intron	30.0%
AAAGCTTCCTTAAGATCTAA+AGG	-	chr3_2:63561683-63561702	None:intergenic	30.0%
TGGTGAAAATTACATGTCAA+AGG	+	chr3_2:63561718-63561737	Msa0416660:CDS	30.0%
AGAACAAAATTGCACCTTAT+TGG	+	chr3_2:63561775-63561794	Msa0416660:CDS	30.0%
AAATTGCACCTTATTGGATT+TGG	+	chr3_2:63561781-63561800	Msa0416660:CDS	30.0%
AAAAAACCATTTGAGCAATG+TGG	+	chr3_2:63562073-63562092	Msa0416660:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_2	gene	63561100	63566218	63561100	ID=Msa0416660;Name=Msa0416660
chr3_2	mRNA	63561100	63566218	63561100	ID=Msa0416660-mRNA-1;Parent=Msa0416660;Name=Msa0416660-mRNA-1;_AED=0.08;_eAED=0.08;_QI=333\|0.8\|0.90\|0.90\|0.9\|0.81\|11\|129\|619
chr3_2	exon	63561100	63561270	63561100	ID=Msa0416660-mRNA-1:exon:13844;Parent=Msa0416660-mRNA-1
chr3_2	exon	63561449	63561934	63561449	ID=Msa0416660-mRNA-1:exon:13845;Parent=Msa0416660-mRNA-1
chr3_2	exon	63563525	63563724	63563525	ID=Msa0416660-mRNA-1:exon:13846;Parent=Msa0416660-mRNA-1
chr3_2	exon	63563826	63563961	63563826	ID=Msa0416660-mRNA-1:exon:13847;Parent=Msa0416660-mRNA-1
chr3_2	exon	63564094	63564171	63564094	ID=Msa0416660-mRNA-1:exon:13848;Parent=Msa0416660-mRNA-1
chr3_2	exon	63564246	63564482	63564246	ID=Msa0416660-mRNA-1:exon:13849;Parent=Msa0416660-mRNA-1
chr3_2	exon	63564563	63564783	63564563	ID=Msa0416660-mRNA-1:exon:13850;Parent=Msa0416660-mRNA-1
chr3_2	exon	63564864	63564981	63564864	ID=Msa0416660-mRNA-1:exon:13851;Parent=Msa0416660-mRNA-1
chr3_2	exon	63565389	63565541	63565389	ID=Msa0416660-mRNA-1:exon:13852;Parent=Msa0416660-mRNA-1
chr3_2	exon	63565621	63565707	63565621	ID=Msa0416660-mRNA-1:exon:13853;Parent=Msa0416660-mRNA-1
chr3_2	exon	63565784	63566218	63565784	ID=Msa0416660-mRNA-1:exon:13854;Parent=Msa0416660-mRNA-1
chr3_2	five_prime_UTR	63561100	63561270	63561100	ID=Msa0416660-mRNA-1:five_prime_utr;Parent=Msa0416660-mRNA-1
chr3_2	five_prime_UTR	63561449	63561610	63561449	ID=Msa0416660-mRNA-1:five_prime_utr;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63561611	63561934	63561611	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63563525	63563724	63563525	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63563826	63563961	63563826	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63564094	63564171	63564094	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63564246	63564482	63564246	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63564563	63564783	63564563	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63564864	63564981	63564864	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63565389	63565541	63565389	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63565621	63565707	63565621	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	CDS	63565784	63566089	63565784	ID=Msa0416660-mRNA-1:cds;Parent=Msa0416660-mRNA-1
chr3_2	three_prime_UTR	63566090	63566218	63566090	ID=Msa0416660-mRNA-1:three_prime_utr;Parent=Msa0416660-mRNA-1

Gene Sequence

>Msa0416660

ATGAAAAAAAAGATCATCAAGTTGTTAAGCTTTTTGACCAAAAAAGGTAAAGTGAATAAGGAAGAACCACCTTTAGATCTTAAGGAAGCTTTTTCCAAGTTTGCAAATGGTGAAAATTACATGTCAAAGGATCAACTACTTAGATTCATGGTGGAATATCAAAGAGAACAAAATTGCACCTTATTGGATTTGGAGCCAATCATTGAAAAGGTTTTGAAAGTGGAAAGTTCTAACAATGAAACTAGCAGTATTGCAGGACTCAGTTTGGATGACTTCATAGATTTTCTGCTTCTAGATGATTTCAATGGTCCCTTAAAGGATGAGGTACATCATGATATGGAAGCTCCCTTGTCACATTATTTCATGTATACAGGACACAATTCCTACCTTACTGGAAATCAATTTACTAGTGAGAGCAGTGATAAACCAATTATAGAGGCTTTGAAACAAGGTGTTCGAGTAATTGAACTTGATTTATGGCCATCTTCAACTAAAGATGGTGGTATTAAAGTAGTTCATGGAAAGACTCTCACCACTCCAGTTGCACTAACCAAATGTTTGGAAGCCATAAAAGAATATGCATTTGTCAAATCTGAATTTCCAGTGATTCTAACTTTAGAAGACCACCTAACACCAAAACTTCAGGATAACTTTGCACAAACACCATCACGTGCCGGACAATATGCATATCTTCAATCTAAAGCATCAGTTCTACAAAGCTTAACCATCTCCTATCTAATGGCAAATAAAATATTTGGAGAAATGCTTTATTGTCCTACGACAGATTGTATAACAGAATTTCCCTCACCAGCATCACTAAAAAATATGGTACTTATATCAACTAAACCACCAAAGGAGCATCCTCAAACTGATGGTGCCAACAATCATGTGTCAAATGGAAGTGAATCGTCTGAAGATGAAACATGGGGACAGGAACAACAAGATTCTATGGCTAAACTGAAAACTGAGGACATGAAAGTAAATGGTGAAGAGATGGAAGATATTAGTACAAGTTATTACAAATCAAACCAGCAAGGTGCGCGTGAATACAGACATTTGATTACAATCCATGGTGGAAAATCAGAAGGTACTATGAAGGATAGACTAAAAGTTGACGGCGGTAAAGTTAAAAGGCTAAGTTTGAGTGAGAAGAAACTCAAATCTGCTTCTGAATCTCATGGAGCCGACCTAATTAGGTTCACGCAGAAAAATATCCTAAGAATTTATCCTAAAGGAGAACGTGTTCAATCCACAAATTTTAGGCCACATTTAGGGTGGATGTATGGAGCTCAGATGGTTGCATTCAATATGCAGGGGCATGGCAAATCACTCCGATTGATGCAAGGGATGTTCAAAGCGAATGGAGGGTGCGGTTATGTGAAAAAACCTGAATTTCTAATTCAAGAAGTTGCAAATAATGAGGCTTTTGATCCTAAAAGAAGGTTTCCAGTGAAGCAGATATTAAAAGTCAAAGTATACAAAGGAGTTGGCTGGAGGTCAGATTTCAGTTCAACGCACTTCGATCGATTCTCTCCACCAGACTTCTACACAAAGGTTTGTATTGCTGGAGTGGGAGCTGATAGTGTGAAGATGAAAACAAGTGTAAAGATGGACAATTGGTATCCTATTTGGGACGAAGAGTTCGAATTCCAATTGACTGTTCCAGAACTCGCGTTGCTCCGGATCGAAGTGAAAGATAAAGATAAGGGATCAGACGATTTTGCTGGACAAACTTGTTTGCCTATATCAGAGCTAAGACATGGATTTCGGTCTGTTGCTTTGTGTGACAGAAAGGGAAAAAAATATAACTCTGTCAAGCTTTTACTTGGATTCCACCTTGAAACTTTAAATATATTGTAG

Protein sequence

>Msa0416660

MKKKIIKLLSFLTKKGKVNKEEPPLDLKEAFSKFANGENYMSKDQLLRFMVEYQREQNCTLLDLEPIIEKVLKVESSNNETSSIAGLSLDDFIDFLLLDDFNGPLKDEVHHDMEAPLSHYFMYTGHNSYLTGNQFTSESSDKPIIEALKQGVRVIELDLWPSSTKDGGIKVVHGKTLTTPVALTKCLEAIKEYAFVKSEFPVILTLEDHLTPKLQDNFAQTPSRAGQYAYLQSKASVLQSLTISYLMANKIFGEMLYCPTTDCITEFPSPASLKNMVLISTKPPKEHPQTDGANNHVSNGSESSEDETWGQEQQDSMAKLKTEDMKVNGEEMEDISTSYYKSNQQGAREYRHLITIHGGKSEGTMKDRLKVDGGKVKRLSLSEKKLKSASESHGADLIRFTQKNILRIYPKGERVQSTNFRPHLGWMYGAQMVAFNMQGHGKSLRLMQGMFKANGGCGYVKKPEFLIQEVANNEAFDPKRRFPVKQILKVKVYKGVGWRSDFSSTHFDRFSPPDFYTKVCIAGVGADSVKMKTSVKMDNWYPIWDEEFEFQLTVPELALLRIEVKDKDKGSDDFAGQTCLPISELRHGFRSVALCDRKGKKYNSVKLLLGFHLETLNIL*