AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0441290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0441290 MtrunA17_Chr3g0078561 89.409 406 18 1 1 381 1 406 0.0 721
Msa0441290 MtrunA17_Chr8g0356011 36.591 440 187 17 1 377 1 411 2.62e-48 168
Msa0441290 MtrunA17_Chr5g0440311 36.735 98 60 2 11 108 4 99 2.18e-13 69.7
Msa0441290 MtrunA17_Chr5g0440291 31.343 134 71 5 19 142 13 135 5.37e-13 68.6
Msa0441290 MtrunA17_Chr3g0078491 27.340 406 234 18 20 380 9 398 2.41e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0441290 AT5G04890.1 44.944 89 48 1 20 108 16 103 1.36e-19 89.7
Msa0441290 AT2G27140.1 32.787 122 79 3 17 138 11 129 9.50e-14 70.5
Msa0441290 AT3G10680.1 32.692 104 69 1 4 107 7 109 1.50e-13 72.4

Find 78 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTTCAACTTGTGATTTA+AGG 0.135801 3_3:+9702500 None:intergenic
AAAGCCTTTAGTTGCAAATA+TGG 0.210552 3_3:-9701786 Msa0441290:CDS
GCTAGAAGAGATAAACTTAT+AGG 0.216530 3_3:+9701721 None:intergenic
CTTCAAGGCAAGTTTGAAAA+TGG 0.223575 3_3:-9702559 Msa0441290:CDS
AAAGTTTCATACACAGGATT+TGG 0.240469 3_3:+9703496 None:intergenic
TTGGTTCCCTTGGGTGTTCT+AGG 0.258214 3_3:+9702454 None:intergenic
ACCTTCTTGTCTCTAATTAT+AGG 0.272544 3_3:+9702394 None:intergenic
CTCCATTAATCCTTAGTGTT+CGG 0.276081 3_3:+9702662 None:intergenic
CCCTTGGGTGTTCTAGGACT+TGG 0.288101 3_3:+9702460 None:intergenic
GAATAAATCAAGAAGCAAAA+AGG 0.292738 3_3:-9703567 Msa0441290:five_prime_UTR
TCCATTAATCCTTAGTGTTC+GGG 0.297124 3_3:+9702663 None:intergenic
TTTCTTCACATTCACCTTCC+TGG 0.298848 3_3:-9703433 Msa0441290:intron
ATCAAAAGGAGAAGAATATT+TGG 0.299073 3_3:-9701972 Msa0441290:CDS
TTAGCAATAAATAAAATAAA+TGG 0.300207 3_3:+9701601 None:intergenic
CAGAATATGATTGGTCAAAT+AGG 0.302570 3_3:+9702608 None:intergenic
AAGTTTCATACACAGGATTT+GGG 0.304860 3_3:+9703497 None:intergenic
TCCCGAACACTAAGGATTAA+TGG 0.308064 3_3:-9702664 Msa0441290:CDS
CTCAAGTTGTCAAGAGAATA+GGG 0.320215 3_3:-9701832 Msa0441290:CDS
TGGAGGCTTTGAAGGAATAT+TGG 0.320768 3_3:+9702435 None:intergenic
TATATTCAACATTTGTTCAT+TGG 0.324970 3_3:-9703607 Msa0441290:five_prime_UTR
TTTAGAAGGAAAAGAAATAA+AGG 0.334192 3_3:-9702008 Msa0441290:CDS
TTTGACTTGGAAAAGTATCT+TGG 0.347158 3_3:+9702302 None:intergenic
TGAAGGAATATTGGTTCCCT+TGG 0.376353 3_3:+9702444 None:intergenic
AACAAGTTGAAGGAACAAAA+AGG 0.378553 3_3:-9702328 Msa0441290:CDS
ACTCTAGTTGGAGGCTTTGA+AGG 0.393913 3_3:+9702427 None:intergenic
TGTTTCAACTTGTGATTTAA+GGG 0.399467 3_3:+9702501 None:intergenic
TTTCTGGAACAGAATATGAT+TGG 0.403613 3_3:+9702599 None:intergenic
TCTCAAGTTGTCAAGAGAAT+AGG 0.415456 3_3:-9701833 Msa0441290:CDS
AACCCTGTCACAATATTACT+TGG 0.426130 3_3:+9702361 None:intergenic
CCAACCCAAGGCAGAATTGA+AGG 0.427450 3_3:-9703473 Msa0441290:CDS
GAAGGAATATTGGTTCCCTT+GGG 0.429823 3_3:+9702445 None:intergenic
TCTCCAAGTAATATTGTGAC+AGG 0.442725 3_3:-9702364 Msa0441290:CDS
CCTTCAATTCTGCCTTGGGT+TGG 0.447147 3_3:+9703473 None:intergenic
GAGGATAAATATCAAATTTG+TGG 0.447215 3_3:-9702692 Msa0441290:CDS
TTTGAATTACATTTCAAGAA+AGG 0.448423 3_3:+9701658 None:intergenic
TTGACTTGGAAAAGTATCTT+GGG 0.454151 3_3:+9702303 None:intergenic
AAGCCTTTAGTTGCAAATAT+GGG 0.459552 3_3:-9701785 Msa0441290:CDS
GGTTCTTCTAGTACTCTAGT+TGG 0.460763 3_3:+9702415 None:intergenic
AGGATAAATATCAAATTTGT+GGG 0.471872 3_3:-9702691 Msa0441290:CDS
GCAAGAAAAGAGTCATCAAA+AGG 0.472949 3_3:-9701986 Msa0441290:CDS
TAGACCTCAAAATGTTTATG+AGG 0.473198 3_3:-9702236 Msa0441290:CDS
GCACCCATATTTGCAACTAA+AGG 0.477234 3_3:+9701782 None:intergenic
TGAGGTCTAGATTCTACAAA+GGG 0.477686 3_3:+9702250 None:intergenic
TCTTCTAGTACTCTAGTTGG+AGG 0.480814 3_3:+9702418 None:intergenic
TCAACTTGTGATTTAAGGGA+TGG 0.486917 3_3:+9702505 None:intergenic
TGAAAAGTATACAAAAGAGA+AGG 0.489401 3_3:-9701885 Msa0441290:CDS
AAAAGATGCAAAGAAGGAAA+AGG 0.493150 3_3:-9701945 Msa0441290:CDS
TGTGAGGTGGAGAAACTTCA+AGG 0.500541 3_3:-9702574 Msa0441290:CDS
AACTACTTGGTAGCAACAAA+TGG 0.500941 3_3:-9702633 Msa0441290:CDS
ATTATATTAATAGCAGAGTC+AGG 0.501722 3_3:+9701555 None:intergenic
ATTCTAGTAATTGTTGCACT+TGG 0.506438 3_3:-9701755 Msa0441290:CDS
GGCTGCAAAAGATGCAAAGA+AGG 0.510641 3_3:-9701951 Msa0441290:CDS
CACTAAGGATTAATGGAGAA+AGG 0.511754 3_3:-9702657 Msa0441290:CDS
ACAATATTACTTGGAGATGA+AGG 0.514944 3_3:+9702370 None:intergenic
TTTCCAAGTCAAAGTCCTAA+GGG 0.518428 3_3:-9702292 Msa0441290:CDS
AAATGACTTGAACAAGTTGA+AGG 0.526624 3_3:-9702338 Msa0441290:CDS
CAACTTGTGATTTAAGGGAT+GGG 0.528671 3_3:+9702506 None:intergenic
ACCTATAATTAGAGACAAGA+AGG 0.529275 3_3:-9702395 Msa0441290:CDS
GGCAGAATTGAAGGAAAATG+AGG 0.534459 3_3:-9703464 Msa0441290:CDS
AATGGAGAAAGGCAACTACT+TGG 0.540238 3_3:-9702646 Msa0441290:CDS
TGGGACTCTCCCGAACACTA+AGG 0.543028 3_3:-9702672 Msa0441290:CDS
CAAAGCAAAGAGGTATAAGA+AGG 0.543303 3_3:-9701909 Msa0441290:CDS
GAAGGGAATCTTCAAAGAAG+TGG 0.546448 3_3:-9701867 Msa0441290:CDS
TTTCCCTTAGGACTTTGACT+TGG 0.546496 3_3:+9702289 None:intergenic
GAAAAGTATACAAAAGAGAA+GGG 0.549687 3_3:-9701884 Msa0441290:CDS
GGTTGGAAAGTTTCATACAC+AGG 0.568545 3_3:+9703490 None:intergenic
TCATACACAGGATTTGGGGA+AGG 0.574199 3_3:+9703502 None:intergenic
TTGAGGTCTAGATTCTACAA+AGG 0.598222 3_3:+9702249 None:intergenic
GCCAAGTCCTAGAACACCCA+AGG 0.612732 3_3:-9702461 Msa0441290:CDS
TGTTCCAGAAAATTGTGAGG+TGG 0.616853 3_3:-9702587 Msa0441290:CDS
GTATGAAACTTTCCAACCCA+AGG 0.630238 3_3:-9703485 Msa0441290:CDS
TCAAGTTGTCAAGAGAATAG+GGG 0.639104 3_3:-9701831 Msa0441290:CDS
CTACAATTGACAAAGCAAAG+AGG 0.640484 3_3:-9701919 Msa0441290:CDS
CTCCAAGTAATATTGTGACA+GGG 0.641452 3_3:-9702363 Msa0441290:CDS
TTCTGTTCCAGAAAATTGTG+AGG 0.646990 3_3:-9702590 Msa0441290:CDS
AGTTTCATACACAGGATTTG+GGG 0.655568 3_3:+9703498 None:intergenic
GTTGTCAAGAGAATAGGGGA+AGG 0.658130 3_3:-9701827 Msa0441290:CDS
CCAAGTCCTAGAACACCCAA+GGG 0.690447 3_3:-9702460 Msa0441290:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGCAATAAATAAAATAAA+TGG + chr3_3:9703578-9703597 None:intergenic 10.0%
!! TTAGCAATAAATAAAATAAA+TGG + chr3_3:9703578-9703597 None:intergenic 10.0%
!!! TTATTTTACTTATTAGTCTT+TGG + chr3_3:9701827-9701846 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG + chr3_3:9703038-9703057 None:intergenic 15.0%
!!! ATTTTATTTATTGCTAAATG+TGG - chr3_3:9703581-9703600 Msa0441290:five_prime_UTR 15.0%
!!! TTTTATTTATTGCTAAATGT+GGG - chr3_3:9703582-9703601 Msa0441290:five_prime_UTR 15.0%
!!! TTATTTTACTTATTAGTCTT+TGG + chr3_3:9701827-9701846 None:intergenic 15.0%
!!! TTTTTTAATTGCTTCTAATA+TGG + chr3_3:9703038-9703057 None:intergenic 15.0%
!!! ATTTTATTTATTGCTAAATG+TGG - chr3_3:9703581-9703600 Msa0441290:five_prime_UTR 15.0%
!!! TTTTATTTATTGCTAAATGT+GGG - chr3_3:9703582-9703601 Msa0441290:five_prime_UTR 15.0%
!! TATATTCAACATTTGTTCAT+TGG - chr3_3:9701569-9701588 Msa0441290:three_prime_UTR 20.0%
!! AATAAATCAAGAAGCAAAAA+GGG - chr3_3:9701610-9701629 Msa0441290:three_prime_UTR 20.0%
!! CTTTAAATTCTACGTAATAT+TGG - chr3_3:9701795-9701814 Msa0441290:CDS 20.0%
!!! TTTGATTTGTTTTTTGTTCT+AGG - chr3_3:9701860-9701879 Msa0441290:CDS 20.0%
!!! TTAACTAAAGTTGATGTATT+CGG + chr3_3:9702344-9702363 None:intergenic 20.0%
!! AGGATAAATATCAAATTTGT+GGG - chr3_3:9702485-9702504 Msa0441290:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG - chr3_3:9703005-9703024 Msa0441290:intron 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG - chr3_3:9703168-9703187 Msa0441290:intron 20.0%
!! TTTGAATTACATTTCAAGAA+AGG + chr3_3:9703521-9703540 None:intergenic 20.0%
!! ATGAACAAATGTTGAATATA+AGG + chr3_3:9701569-9701588 None:intergenic 20.0%
!! TATATTCAACATTTGTTCAT+TGG - chr3_3:9701569-9701588 Msa0441290:three_prime_UTR 20.0%
!! AATAAATCAAGAAGCAAAAA+GGG - chr3_3:9701610-9701629 Msa0441290:three_prime_UTR 20.0%
!! CTTTAAATTCTACGTAATAT+TGG - chr3_3:9701795-9701814 Msa0441290:CDS 20.0%
!!! TTTGATTTGTTTTTTGTTCT+AGG - chr3_3:9701860-9701879 Msa0441290:CDS 20.0%
!!! TTAACTAAAGTTGATGTATT+CGG + chr3_3:9702344-9702363 None:intergenic 20.0%
!! AGGATAAATATCAAATTTGT+GGG - chr3_3:9702485-9702504 Msa0441290:CDS 20.0%
!! AAAAAATTCAAGAGGAAAAT+AGG - chr3_3:9703005-9703024 Msa0441290:intron 20.0%
!! TTTAGAAGGAAAAGAAATAA+AGG - chr3_3:9703168-9703187 Msa0441290:intron 20.0%
!! TTTGAATTACATTTCAAGAA+AGG + chr3_3:9703521-9703540 None:intergenic 20.0%
! GAATAAATCAAGAAGCAAAA+AGG - chr3_3:9701609-9701628 Msa0441290:three_prime_UTR 25.0%
! TAAATTCTACGTAATATTGG+AGG - chr3_3:9701798-9701817 Msa0441290:CDS 25.0%
! AAATTCTACGTAATATTGGA+GGG - chr3_3:9701799-9701818 Msa0441290:CDS 25.0%
! AAATGTGATCCTTTCTAATA+TGG + chr3_3:9701898-9701917 None:intergenic 25.0%
!! CTTTTACAAAAAGGATCAAA+GGG + chr3_3:9702276-9702295 None:intergenic 25.0%
!! TCTTTTACAAAAAGGATCAA+AGG + chr3_3:9702277-9702296 None:intergenic 25.0%
! GAGGATAAATATCAAATTTG+TGG - chr3_3:9702484-9702503 Msa0441290:CDS 25.0%
! TGTTTCAACTTGTGATTTAA+GGG + chr3_3:9702678-9702697 None:intergenic 25.0%
! TTGTTTCAACTTGTGATTTA+AGG + chr3_3:9702679-9702698 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG + chr3_3:9702947-9702966 None:intergenic 25.0%
!! TTTTTCATCATCTATTGCTT+GGG + chr3_3:9702990-9703009 None:intergenic 25.0%
!!! TTTTTTCATCATCTATTGCT+TGG + chr3_3:9702991-9703010 None:intergenic 25.0%
! AGATGATGAAAAAATTCAAG+AGG - chr3_3:9702997-9703016 Msa0441290:intron 25.0%
! AATTAAAAAACAATTGCACG+AGG - chr3_3:9703048-9703067 Msa0441290:intron 25.0%
! ATCAAAAGGAGAAGAATATT+TGG - chr3_3:9703204-9703223 Msa0441290:intron 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG - chr3_3:9703291-9703310 Msa0441290:intron 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG - chr3_3:9703292-9703311 Msa0441290:intron 25.0%
! GAATAAATCAAGAAGCAAAA+AGG - chr3_3:9701609-9701628 Msa0441290:three_prime_UTR 25.0%
! TAAATTCTACGTAATATTGG+AGG - chr3_3:9701798-9701817 Msa0441290:CDS 25.0%
! AAATTCTACGTAATATTGGA+GGG - chr3_3:9701799-9701818 Msa0441290:CDS 25.0%
! AAATGTGATCCTTTCTAATA+TGG + chr3_3:9701898-9701917 None:intergenic 25.0%
!! CTTTTACAAAAAGGATCAAA+GGG + chr3_3:9702276-9702295 None:intergenic 25.0%
!! TCTTTTACAAAAAGGATCAA+AGG + chr3_3:9702277-9702296 None:intergenic 25.0%
! GAGGATAAATATCAAATTTG+TGG - chr3_3:9702484-9702503 Msa0441290:CDS 25.0%
! TGTTTCAACTTGTGATTTAA+GGG + chr3_3:9702678-9702697 None:intergenic 25.0%
! TTGTTTCAACTTGTGATTTA+AGG + chr3_3:9702679-9702698 None:intergenic 25.0%
!! TTTTCCTCATAAACATTTTG+AGG + chr3_3:9702947-9702966 None:intergenic 25.0%
!! TTTTTCATCATCTATTGCTT+GGG + chr3_3:9702990-9703009 None:intergenic 25.0%
!!! TTTTTTCATCATCTATTGCT+TGG + chr3_3:9702991-9703010 None:intergenic 25.0%
! AGATGATGAAAAAATTCAAG+AGG - chr3_3:9702997-9703016 Msa0441290:intron 25.0%
! AATTAAAAAACAATTGCACG+AGG - chr3_3:9703048-9703067 Msa0441290:intron 25.0%
! ATCAAAAGGAGAAGAATATT+TGG - chr3_3:9703204-9703223 Msa0441290:intron 25.0%
!! TGAAAAGTATACAAAAGAGA+AGG - chr3_3:9703291-9703310 Msa0441290:intron 25.0%
!! GAAAAGTATACAAAAGAGAA+GGG - chr3_3:9703292-9703311 Msa0441290:intron 25.0%
AAGTTTCATACACAGGATTT+GGG + chr3_3:9701682-9701701 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG + chr3_3:9701683-9701702 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG + chr3_3:9701764-9701783 None:intergenic 30.0%
AATTCTACGTAATATTGGAG+GGG - chr3_3:9701800-9701819 Msa0441290:CDS 30.0%
!! TTGATTTGGTTGTATTGACT+TGG - chr3_3:9702027-9702046 Msa0441290:CDS 30.0%
!! TGATTTGGTTGTATTGACTT+GGG - chr3_3:9702028-9702047 Msa0441290:CDS 30.0%
! CCTACTTGTCTTTTACAAAA+AGG + chr3_3:9702285-9702304 None:intergenic 30.0%
! CCTTTTTGTAAAAGACAAGT+AGG - chr3_3:9702282-9702301 Msa0441290:CDS 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG - chr3_3:9702460-9702479 Msa0441290:CDS 30.0%
! CAGAATATGATTGGTCAAAT+AGG + chr3_3:9702571-9702590 None:intergenic 30.0%
!! TTTCTGGAACAGAATATGAT+TGG + chr3_3:9702580-9702599 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG + chr3_3:9702702-9702721 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG - chr3_3:9702781-9702800 Msa0441290:intron 30.0%
ACCTTCTTGTCTCTAATTAT+AGG + chr3_3:9702785-9702804 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG + chr3_3:9702809-9702828 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG - chr3_3:9702838-9702857 Msa0441290:intron 30.0%
! AACAAGTTGAAGGAACAAAA+AGG - chr3_3:9702848-9702867 Msa0441290:intron 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG + chr3_3:9702876-9702895 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG + chr3_3:9702877-9702896 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG + chr3_3:9702902-9702921 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG - chr3_3:9702940-9702959 Msa0441290:intron 30.0%
!! GATCACAATACGTTTTTAGA+AGG - chr3_3:9703154-9703173 Msa0441290:intron 30.0%
AAAAGATGCAAAGAAGGAAA+AGG - chr3_3:9703231-9703250 Msa0441290:intron 30.0%
AAAGCCTTTAGTTGCAAATA+TGG - chr3_3:9703390-9703409 Msa0441290:intron 30.0%
AAGCCTTTAGTTGCAAATAT+GGG - chr3_3:9703391-9703410 Msa0441290:intron 30.0%
ATTCTAGTAATTGTTGCACT+TGG - chr3_3:9703421-9703440 Msa0441290:intron 30.0%
GCTAGAAGAGATAAACTTAT+AGG + chr3_3:9703458-9703477 None:intergenic 30.0%
AAGTTTCATACACAGGATTT+GGG + chr3_3:9701682-9701701 None:intergenic 30.0%
AAAGTTTCATACACAGGATT+TGG + chr3_3:9701683-9701702 None:intergenic 30.0%
AAAAACAAAAAATACGCACC+AGG + chr3_3:9701764-9701783 None:intergenic 30.0%
AATTCTACGTAATATTGGAG+GGG - chr3_3:9701800-9701819 Msa0441290:CDS 30.0%
!! TTGATTTGGTTGTATTGACT+TGG - chr3_3:9702027-9702046 Msa0441290:CDS 30.0%
!! TGATTTGGTTGTATTGACTT+GGG - chr3_3:9702028-9702047 Msa0441290:CDS 30.0%
! CCTACTTGTCTTTTACAAAA+AGG + chr3_3:9702285-9702304 None:intergenic 30.0%
! CCTTTTTGTAAAAGACAAGT+AGG - chr3_3:9702282-9702301 Msa0441290:CDS 30.0%
!! TGTTCTGCAAGGTTTTATAA+AGG - chr3_3:9702460-9702479 Msa0441290:CDS 30.0%
! CAGAATATGATTGGTCAAAT+AGG + chr3_3:9702571-9702590 None:intergenic 30.0%
!! TTTCTGGAACAGAATATGAT+TGG + chr3_3:9702580-9702599 None:intergenic 30.0%
!!! ACTTGGCACAATTTTTTCTT+TGG + chr3_3:9702702-9702721 None:intergenic 30.0%
ACCTATAATTAGAGACAAGA+AGG - chr3_3:9702781-9702800 Msa0441290:intron 30.0%
ACCTTCTTGTCTCTAATTAT+AGG + chr3_3:9702785-9702804 None:intergenic 30.0%
ACAATATTACTTGGAGATGA+AGG + chr3_3:9702809-9702828 None:intergenic 30.0%
! AAATGACTTGAACAAGTTGA+AGG - chr3_3:9702838-9702857 Msa0441290:intron 30.0%
! AACAAGTTGAAGGAACAAAA+AGG - chr3_3:9702848-9702867 Msa0441290:intron 30.0%
!! TTGACTTGGAAAAGTATCTT+GGG + chr3_3:9702876-9702895 None:intergenic 30.0%
!! TTTGACTTGGAAAAGTATCT+TGG + chr3_3:9702877-9702896 None:intergenic 30.0%
! AAAGCTTTCAATTTTCCCTT+AGG + chr3_3:9702902-9702921 None:intergenic 30.0%
TAGACCTCAAAATGTTTATG+AGG - chr3_3:9702940-9702959 Msa0441290:intron 30.0%
!! GATCACAATACGTTTTTAGA+AGG - chr3_3:9703154-9703173 Msa0441290:intron 30.0%
AAAAGATGCAAAGAAGGAAA+AGG - chr3_3:9703231-9703250 Msa0441290:intron 30.0%
AAAGCCTTTAGTTGCAAATA+TGG - chr3_3:9703390-9703409 Msa0441290:intron 30.0%
AAGCCTTTAGTTGCAAATAT+GGG - chr3_3:9703391-9703410 Msa0441290:intron 30.0%
ATTCTAGTAATTGTTGCACT+TGG - chr3_3:9703421-9703440 Msa0441290:intron 30.0%
GCTAGAAGAGATAAACTTAT+AGG + chr3_3:9703458-9703477 None:intergenic 30.0%
AGTTTCATACACAGGATTTG+GGG + chr3_3:9701681-9701700 None:intergenic 35.0%
! TTTTCCTTCAATTCTGCCTT+GGG + chr3_3:9701710-9701729 None:intergenic 35.0%
! ATTTTCCTTCAATTCTGCCT+TGG + chr3_3:9701711-9701730 None:intergenic 35.0%
TGTGATCCTTTCTAATATGG+AGG + chr3_3:9701895-9701914 None:intergenic 35.0%
TTGTCTTAAGAAAACCCACT+TGG - chr3_3:9702003-9702022 Msa0441290:CDS 35.0%
TGTCTTAAGAAAACCCACTT+GGG - chr3_3:9702004-9702023 Msa0441290:CDS 35.0%
ATTCTCCTCGATGTCAATTT+GGG - chr3_3:9702090-9702109 Msa0441290:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG - chr3_3:9702465-9702484 Msa0441290:CDS 35.0%
TCCATTAATCCTTAGTGTTC+GGG + chr3_3:9702516-9702535 None:intergenic 35.0%
CTCCATTAATCCTTAGTGTT+CGG + chr3_3:9702517-9702536 None:intergenic 35.0%
CACTAAGGATTAATGGAGAA+AGG - chr3_3:9702519-9702538 Msa0441290:CDS 35.0%
! AACTACTTGGTAGCAACAAA+TGG - chr3_3:9702543-9702562 Msa0441290:CDS 35.0%
TTCTGTTCCAGAAAATTGTG+AGG - chr3_3:9702586-9702605 Msa0441290:CDS 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG - chr3_3:9702617-9702636 Msa0441290:CDS 35.0%
!!! TTAAGGGATGGGAACTTTTT+TGG + chr3_3:9702662-9702681 None:intergenic 35.0%
CAACTTGTGATTTAAGGGAT+GGG + chr3_3:9702673-9702692 None:intergenic 35.0%
TCAACTTGTGATTTAAGGGA+TGG + chr3_3:9702674-9702693 None:intergenic 35.0%
TCTCCAAGTAATATTGTGAC+AGG - chr3_3:9702812-9702831 Msa0441290:intron 35.0%
CTCCAAGTAATATTGTGACA+GGG - chr3_3:9702813-9702832 Msa0441290:intron 35.0%
AACCCTGTCACAATATTACT+TGG + chr3_3:9702818-9702837 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG - chr3_3:9702883-9702902 Msa0441290:intron 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG - chr3_3:9702884-9702903 Msa0441290:intron 35.0%
TGAGGTCTAGATTCTACAAA+GGG + chr3_3:9702929-9702948 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG + chr3_3:9702930-9702949 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+AGG - chr3_3:9703190-9703209 Msa0441290:intron 35.0%
CTACAATTGACAAAGCAAAG+AGG - chr3_3:9703257-9703276 Msa0441290:intron 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG - chr3_3:9703267-9703286 Msa0441290:intron 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG - chr3_3:9703343-9703362 Msa0441290:intron 35.0%
! CTCAAGTTGTCAAGAGAATA+GGG - chr3_3:9703344-9703363 Msa0441290:intron 35.0%
! TCAAGTTGTCAAGAGAATAG+GGG - chr3_3:9703345-9703364 Msa0441290:intron 35.0%
AGTTTCATACACAGGATTTG+GGG + chr3_3:9701681-9701700 None:intergenic 35.0%
! TTTTCCTTCAATTCTGCCTT+GGG + chr3_3:9701710-9701729 None:intergenic 35.0%
! ATTTTCCTTCAATTCTGCCT+TGG + chr3_3:9701711-9701730 None:intergenic 35.0%
TGTGATCCTTTCTAATATGG+AGG + chr3_3:9701895-9701914 None:intergenic 35.0%
TTGTCTTAAGAAAACCCACT+TGG - chr3_3:9702003-9702022 Msa0441290:CDS 35.0%
TGTCTTAAGAAAACCCACTT+GGG - chr3_3:9702004-9702023 Msa0441290:CDS 35.0%
ATTCTCCTCGATGTCAATTT+GGG - chr3_3:9702090-9702109 Msa0441290:CDS 35.0%
!! TGCAAGGTTTTATAAAGGAG+AGG - chr3_3:9702465-9702484 Msa0441290:CDS 35.0%
TCCATTAATCCTTAGTGTTC+GGG + chr3_3:9702516-9702535 None:intergenic 35.0%
CTCCATTAATCCTTAGTGTT+CGG + chr3_3:9702517-9702536 None:intergenic 35.0%
CACTAAGGATTAATGGAGAA+AGG - chr3_3:9702519-9702538 Msa0441290:CDS 35.0%
! AACTACTTGGTAGCAACAAA+TGG - chr3_3:9702543-9702562 Msa0441290:CDS 35.0%
TTCTGTTCCAGAAAATTGTG+AGG - chr3_3:9702586-9702605 Msa0441290:CDS 35.0%
CTTCAAGGCAAGTTTGAAAA+TGG - chr3_3:9702617-9702636 Msa0441290:CDS 35.0%
!!! TTAAGGGATGGGAACTTTTT+TGG + chr3_3:9702662-9702681 None:intergenic 35.0%
CAACTTGTGATTTAAGGGAT+GGG + chr3_3:9702673-9702692 None:intergenic 35.0%
TCAACTTGTGATTTAAGGGA+TGG + chr3_3:9702674-9702693 None:intergenic 35.0%
TCTCCAAGTAATATTGTGAC+AGG - chr3_3:9702812-9702831 Msa0441290:intron 35.0%
CTCCAAGTAATATTGTGACA+GGG - chr3_3:9702813-9702832 Msa0441290:intron 35.0%
AACCCTGTCACAATATTACT+TGG + chr3_3:9702818-9702837 None:intergenic 35.0%
! TTTTCCAAGTCAAAGTCCTA+AGG - chr3_3:9702883-9702902 Msa0441290:intron 35.0%
TTTCCAAGTCAAAGTCCTAA+GGG - chr3_3:9702884-9702903 Msa0441290:intron 35.0%
TGAGGTCTAGATTCTACAAA+GGG + chr3_3:9702929-9702948 None:intergenic 35.0%
TTGAGGTCTAGATTCTACAA+AGG + chr3_3:9702930-9702949 None:intergenic 35.0%
GCAAGAAAAGAGTCATCAAA+AGG - chr3_3:9703190-9703209 Msa0441290:intron 35.0%
CTACAATTGACAAAGCAAAG+AGG - chr3_3:9703257-9703276 Msa0441290:intron 35.0%
! CAAAGCAAAGAGGTATAAGA+AGG - chr3_3:9703267-9703286 Msa0441290:intron 35.0%
! TCTCAAGTTGTCAAGAGAAT+AGG - chr3_3:9703343-9703362 Msa0441290:intron 35.0%
! CTCAAGTTGTCAAGAGAATA+GGG - chr3_3:9703344-9703363 Msa0441290:intron 35.0%
! TCAAGTTGTCAAGAGAATAG+GGG - chr3_3:9703345-9703364 Msa0441290:intron 35.0%
TCAAGAAGCAAAAAGGGCTA+TGG - chr3_3:9701616-9701635 Msa0441290:three_prime_UTR 40.0%
!!! GAAGGTTTTGTTCTAGAGGT+AGG + chr3_3:9701659-9701678 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG + chr3_3:9701689-9701708 None:intergenic 40.0%
GTATGAAACTTTCCAACCCA+AGG - chr3_3:9701691-9701710 Msa0441290:three_prime_UTR 40.0%
GGCAGAATTGAAGGAAAATG+AGG - chr3_3:9701712-9701731 Msa0441290:CDS 40.0%
TTTCTTCACATTCACCTTCC+TGG - chr3_3:9701743-9701762 Msa0441290:CDS 40.0%
ACAAAAAATACGCACCAGGA+AGG + chr3_3:9701760-9701779 None:intergenic 40.0%
TGCACTCCTCCATATTAGAA+AGG - chr3_3:9701886-9701905 Msa0441290:CDS 40.0%
!! AAAACCCACTTGGGTTGATT+TGG - chr3_3:9702013-9702032 Msa0441290:CDS 40.0%
ACAACCAAATCAACCCAAGT+GGG + chr3_3:9702020-9702039 None:intergenic 40.0%
TACAACCAAATCAACCCAAG+TGG + chr3_3:9702021-9702040 None:intergenic 40.0%
! TGAGAGTGTACTCATTCTCA+AGG - chr3_3:9702056-9702075 Msa0441290:CDS 40.0%
GATTCTCCTCGATGTCAATT+TGG - chr3_3:9702089-9702108 Msa0441290:CDS 40.0%
TATCACCCAAATTGACATCG+AGG + chr3_3:9702098-9702117 None:intergenic 40.0%
TCCCGAACACTAAGGATTAA+TGG - chr3_3:9702512-9702531 Msa0441290:CDS 40.0%
AATGGAGAAAGGCAACTACT+TGG - chr3_3:9702530-9702549 Msa0441290:CDS 40.0%
TGTTCCAGAAAATTGTGAGG+TGG - chr3_3:9702589-9702608 Msa0441290:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG + chr3_3:9702596-9702615 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG + chr3_3:9702734-9702753 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG + chr3_3:9702735-9702754 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG + chr3_3:9702744-9702763 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG + chr3_3:9702761-9702780 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG + chr3_3:9702764-9702783 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG + chr3_3:9702890-9702909 None:intergenic 40.0%
GAAGGGAATCTTCAAAGAAG+TGG - chr3_3:9703309-9703328 Msa0441290:intron 40.0%
GCACCCATATTTGCAACTAA+AGG + chr3_3:9703397-9703416 None:intergenic 40.0%
TCAAGAAGCAAAAAGGGCTA+TGG - chr3_3:9701616-9701635 Msa0441290:three_prime_UTR 40.0%
!!! GAAGGTTTTGTTCTAGAGGT+AGG + chr3_3:9701659-9701678 None:intergenic 40.0%
GGTTGGAAAGTTTCATACAC+AGG + chr3_3:9701689-9701708 None:intergenic 40.0%
GTATGAAACTTTCCAACCCA+AGG - chr3_3:9701691-9701710 Msa0441290:three_prime_UTR 40.0%
GGCAGAATTGAAGGAAAATG+AGG - chr3_3:9701712-9701731 Msa0441290:CDS 40.0%
TTTCTTCACATTCACCTTCC+TGG - chr3_3:9701743-9701762 Msa0441290:CDS 40.0%
ACAAAAAATACGCACCAGGA+AGG + chr3_3:9701760-9701779 None:intergenic 40.0%
TGCACTCCTCCATATTAGAA+AGG - chr3_3:9701886-9701905 Msa0441290:CDS 40.0%
!! AAAACCCACTTGGGTTGATT+TGG - chr3_3:9702013-9702032 Msa0441290:CDS 40.0%
ACAACCAAATCAACCCAAGT+GGG + chr3_3:9702020-9702039 None:intergenic 40.0%
TACAACCAAATCAACCCAAG+TGG + chr3_3:9702021-9702040 None:intergenic 40.0%
! TGAGAGTGTACTCATTCTCA+AGG - chr3_3:9702056-9702075 Msa0441290:CDS 40.0%
GATTCTCCTCGATGTCAATT+TGG - chr3_3:9702089-9702108 Msa0441290:CDS 40.0%
TATCACCCAAATTGACATCG+AGG + chr3_3:9702098-9702117 None:intergenic 40.0%
TCCCGAACACTAAGGATTAA+TGG - chr3_3:9702512-9702531 Msa0441290:CDS 40.0%
AATGGAGAAAGGCAACTACT+TGG - chr3_3:9702530-9702549 Msa0441290:CDS 40.0%
TGTTCCAGAAAATTGTGAGG+TGG - chr3_3:9702589-9702608 Msa0441290:CDS 40.0%
! TTCTCCACCTCACAATTTTC+TGG + chr3_3:9702596-9702615 None:intergenic 40.0%
!! GAAGGAATATTGGTTCCCTT+GGG + chr3_3:9702734-9702753 None:intergenic 40.0%
!! TGAAGGAATATTGGTTCCCT+TGG + chr3_3:9702735-9702754 None:intergenic 40.0%
!! TGGAGGCTTTGAAGGAATAT+TGG + chr3_3:9702744-9702763 None:intergenic 40.0%
TCTTCTAGTACTCTAGTTGG+AGG + chr3_3:9702761-9702780 None:intergenic 40.0%
GGTTCTTCTAGTACTCTAGT+TGG + chr3_3:9702764-9702783 None:intergenic 40.0%
TTTCCCTTAGGACTTTGACT+TGG + chr3_3:9702890-9702909 None:intergenic 40.0%
GAAGGGAATCTTCAAAGAAG+TGG - chr3_3:9703309-9703328 Msa0441290:intron 40.0%
GCACCCATATTTGCAACTAA+AGG + chr3_3:9703397-9703416 None:intergenic 40.0%
!!! TGGGGAAGGTTTTGTTCTAG+AGG + chr3_3:9701663-9701682 None:intergenic 45.0%
TCATACACAGGATTTGGGGA+AGG + chr3_3:9701677-9701696 None:intergenic 45.0%
GAGAATGAGTACACTCTCAC+AGG + chr3_3:9702055-9702074 None:intergenic 45.0%
CTTCTCTCTCATGTTCTGCA+AGG - chr3_3:9702449-9702468 Msa0441290:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG - chr3_3:9702602-9702621 Msa0441290:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG + chr3_3:9702752-9702771 None:intergenic 45.0%
GGCTGCAAAAGATGCAAAGA+AGG - chr3_3:9703225-9703244 Msa0441290:intron 45.0%
GTTGTCAAGAGAATAGGGGA+AGG - chr3_3:9703349-9703368 Msa0441290:intron 45.0%
!!! TGGGGAAGGTTTTGTTCTAG+AGG + chr3_3:9701663-9701682 None:intergenic 45.0%
TCATACACAGGATTTGGGGA+AGG + chr3_3:9701677-9701696 None:intergenic 45.0%
GAGAATGAGTACACTCTCAC+AGG + chr3_3:9702055-9702074 None:intergenic 45.0%
CTTCTCTCTCATGTTCTGCA+AGG - chr3_3:9702449-9702468 Msa0441290:CDS 45.0%
TGTGAGGTGGAGAAACTTCA+AGG - chr3_3:9702602-9702621 Msa0441290:CDS 45.0%
! ACTCTAGTTGGAGGCTTTGA+AGG + chr3_3:9702752-9702771 None:intergenic 45.0%
GGCTGCAAAAGATGCAAAGA+AGG - chr3_3:9703225-9703244 Msa0441290:intron 45.0%
GTTGTCAAGAGAATAGGGGA+AGG - chr3_3:9703349-9703368 Msa0441290:intron 45.0%
CCTTCAATTCTGCCTTGGGT+TGG + chr3_3:9701706-9701725 None:intergenic 50.0%
CCAACCCAAGGCAGAATTGA+AGG - chr3_3:9701703-9701722 Msa0441290:exon 50.0%
CCAAGTCCTAGAACACCCAA+GGG - chr3_3:9702716-9702735 Msa0441290:intron 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG + chr3_3:9702725-9702744 None:intergenic 50.0%
CCTTCAATTCTGCCTTGGGT+TGG + chr3_3:9701706-9701725 None:intergenic 50.0%
CCAACCCAAGGCAGAATTGA+AGG - chr3_3:9701703-9701722 Msa0441290:exon 50.0%
CCAAGTCCTAGAACACCCAA+GGG - chr3_3:9702716-9702735 Msa0441290:intron 50.0%
TTGGTTCCCTTGGGTGTTCT+AGG + chr3_3:9702725-9702744 None:intergenic 50.0%
TGGGACTCTCCCGAACACTA+AGG - chr3_3:9702504-9702523 Msa0441290:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG - chr3_3:9702715-9702734 Msa0441290:intron 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG + chr3_3:9702719-9702738 None:intergenic 55.0%
TGGGACTCTCCCGAACACTA+AGG - chr3_3:9702504-9702523 Msa0441290:CDS 55.0%
GCCAAGTCCTAGAACACCCA+AGG - chr3_3:9702715-9702734 Msa0441290:intron 55.0%
!! CCCTTGGGTGTTCTAGGACT+TGG + chr3_3:9702719-9702738 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 9701567 9703631 9701567 ID=Msa0441290;Name=Msa0441290
chr3_3 mRNA 9701567 9703631 9701567 ID=Msa0441290-mRNA-1;Parent=Msa0441290;Name=Msa0441290-mRNA-1;_AED=0.01;_eAED=0.01;_QI=68|1|1|1|1|1|2|145|381
chr3_3 exon 9701567 9702727 9701567 ID=Msa0441290-mRNA-1:exon:2023;Parent=Msa0441290-mRNA-1
chr3_3 exon 9703434 9703631 9703434 ID=Msa0441290-mRNA-1:exon:2022;Parent=Msa0441290-mRNA-1
chr3_3 five_prime_UTR 9703564 9703631 9703564 ID=Msa0441290-mRNA-1:five_prime_utr;Parent=Msa0441290-mRNA-1
chr3_3 CDS 9703434 9703563 9703434 ID=Msa0441290-mRNA-1:cds;Parent=Msa0441290-mRNA-1
chr3_3 CDS 9701712 9702727 9701712 ID=Msa0441290-mRNA-1:cds;Parent=Msa0441290-mRNA-1
chr3_3 three_prime_UTR 9701567 9701711 9701567 ID=Msa0441290-mRNA-1:three_prime_utr;Parent=Msa0441290-mRNA-1
Gene Sequence

>Msa0441290

ATGGCTATGAAATCAAGAACACCTACCTCTAGAACAAAACCTTCCCCAAATCCTGTGTATGAAACTTTCCAACCCAAGGCAGAATTGAAGGAAAATGAGGAAGCATATTTTCTTCACATTCACCTTCCTGGTTTTATAAAGGAGAGGATAAATATCAAATTTGTGGGACTCTCCCGAACACTAAGGATTAATGGAGAAAGGCAACTACTTGGTAGCAACAAATGGAGCCTATTTGACCAATCATATTCTGTTCCAGAAAATTGTGAGGTGGAGAAACTTCAAGGCAAGTTTGAAAATGGAACTCTCATTGTTGCAATGCCAAAAAAGTTCCCATCCCTTAAATCACAAGTTGAAACAACCAAAGAAAAAATTGTGCCAAGTCCTAGAACACCCAAGGGAACCAATATTCCTTCAAAGCCTCCAACTAGAGTACTAGAAGAACCTATAATTAGAGACAAGAAGGTTCCTTCATCTCCAAGTAATATTGTGACAGGGTTAAATGACTTGAACAAGTTGAAGGAACAAAAAGGTACCCAAGATACTTTTCCAAGTCAAAGTCCTAAGGGAAAATTGAAAGCTTTAGAACCCTTTGTAGAATCTAGACCTCAAAATGTTTATGAGGAAAATAATGTGCTAAAAGCAAAATCCCAAGCAATAGATGATGAAAAAATTCAAGAGGAAAATAGGAAAAAGTCCATATTAGAAGCAATTAAAAAACAATTGCACGAGGACGAAAAGAAAAGCGTCACAAATAAGAAAATTGAAGAAGATAAGAAGACTTACGAGTCAAGCAAGCTTGATCAACAATACGTTGATCACAATACGTTTTTAGAAGGAAAAGAAATAAAGGCAAGAAAAGAGTCATCAAAAGGAGAAGAATATTTGGCTGCAAAAGATGCAAAGAAGGAAAAGGATACTACAATTGACAAAGCAAAGAGGTATAAGAAGGATGAAAAGTATACAAAAGAGAAGGGAATCTTCAAAGAAGTGGCAACTTCAGCTTCTCAAGTTGTCAAGAGAATAGGGGAAGGAAAATTGAATGAACAAGAAAAGCCTTTAGTTGCAAATATGGGTGCTGCAATTCTAGTAATTGTTGCACTTGGAGCTTATGCTACCTATAAGTTTATCTCTTCTAGCAAAAACTAA
Protein sequence

>Msa0441290

MAMKSRTPTSRTKPSPNPVYETFQPKAELKENEEAYFLHIHLPGFIKERINIKFVGLSRTLRINGERQLLGSNKWSLFDQSYSVPENCEVEKLQGKFENGTLIVAMPKKFPSLKSQVETTKEKIVPSPRTPKGTNIPSKPPTRVLEEPIIRDKKVPSSPSNIVTGLNDLNKLKEQKGTQDTFPSQSPKGKLKALEPFVESRPQNVYEENNVLKAKSQAIDDEKIQEENRKKSILEAIKKQLHEDEKKSVTNKKIEEDKKTYESSKLDQQYVDHNTFLEGKEIKARKESSKGEEYLAAKDAKKEKDTTIDKAKRYKKDEKYTKEKGIFKEVATSASQVVKRIGEGKLNEQEKPLVANMGAAILVIVALGAYATYKFISSSKN*