AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0742650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742650 MtrunA17_Chr5g0444551 97.297 148 4 0 1 148 1 148 5.14e-103 291
Msa0742650 MtrunA17_Chr5g0444571 96.809 94 3 0 1 94 1 94 5.85e-60 180
Msa0742650 MtrunA17_Chr4g0024941 48.936 141 72 0 1 141 1 141 2.62e-45 147
Msa0742650 MtrunA17_Chr2g0288831 50.694 144 70 1 1 143 1 144 3.85e-44 144
Msa0742650 MtrunA17_Chr2g0322421 47.287 129 67 1 1 128 1 129 3.19e-32 114
Msa0742650 MtrunA17_Chr7g0235621 40.458 131 77 1 1 130 1 131 5.90e-30 108
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742650 AT5G42570.1 51.064 141 69 0 1 141 1 141 1.18e-45 148
Msa0742650 AT1G11905.1 50.355 141 70 0 1 141 1 141 7.80e-44 144
Msa0742650 AT3G20450.1 46.512 129 68 1 1 128 5 133 1.17e-37 125
Msa0742650 AT1G11905.2 43.972 141 78 1 1 141 1 140 7.21e-34 118
Msa0742650 AT5G48660.1 42.969 128 72 1 1 127 1 128 3.64e-30 108
Msa0742650 AT3G07190.1 44.531 128 70 1 1 127 1 128 1.33e-29 107
Msa0742650 AT5G48660.2 48.077 52 27 0 76 127 1 52 2.98e-11 58.2

Find 32 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACATCCATTGAACGTTTA+TGG 0.094726 5_1:-62611008 None:intergenic
ACCACTGTATTTGTTGTATT+TGG 0.123258 5_1:+62610958 Msa0742650:CDS
TCCTCTATCTCTCTTCTCTT+TGG 0.214919 5_1:-62611601 None:intergenic
ACAGGTTTCTCTCTATTCTT+TGG 0.261214 5_1:+62611481 Msa0742650:intron
AAATCCAATACGAAAATTAA+TGG 0.270501 5_1:+62610876 Msa0742650:CDS
TTCTCGATTTGGGAGTTTCT+TGG 0.274478 5_1:-62611752 None:intergenic
TTGATATGCAATTCTCGATT+TGG 0.291370 5_1:-62611763 None:intergenic
TCTCTTAGAAGCATCTCTTA+TGG 0.322946 5_1:+62611071 Msa0742650:CDS
AGAGGAAACTGAGAAGAAAA+AGG 0.332303 5_1:+62611618 Msa0742650:CDS
TACAAAATAAGAGTTTGATT+TGG 0.335189 5_1:+62610729 Msa0742650:five_prime_UTR
TGATATGCAATTCTCGATTT+GGG 0.354851 5_1:-62611762 None:intergenic
CTCTTAGAAGCATCTCTTAT+GGG 0.365951 5_1:+62611072 Msa0742650:CDS
GTACACATACAGACATGTTT+AGG 0.407202 5_1:-62611679 None:intergenic
TCTATCTCTCTTCTCTTTGG+AGG 0.415834 5_1:-62611598 None:intergenic
TCCAACAGAGGAGGTTCTAA+TGG 0.420935 5_1:+62611041 Msa0742650:CDS
AAAATAAGAGTTTGATTTGG+AGG 0.430028 5_1:+62610732 Msa0742650:five_prime_UTR
CAAAATCCATAAACGTTCAA+TGG 0.441548 5_1:+62611002 Msa0742650:CDS
TCCAAAGAGAAGAGAGATAG+AGG 0.454256 5_1:+62611600 Msa0742650:CDS
GACCCCTTCCACGCTTCAAA+AGG 0.463134 5_1:-62610912 None:intergenic
GCCATTAGAACCTCCTCTGT+TGG 0.483298 5_1:-62611042 None:intergenic
TACTTGAATGTTGGTTTCGA+TGG 0.490667 5_1:-62610777 None:intergenic
CATAAACGTTCAATGGATGT+TGG 0.531550 5_1:+62611009 Msa0742650:CDS
TTGTAGAACTACTTGAATGT+TGG 0.532246 5_1:-62610786 None:intergenic
TCTTTGGATTGATGATCGAT+AGG 0.543666 5_1:+62611497 Msa0742650:CDS
ACGTTCAATGGATGTTGGCA+TGG 0.548399 5_1:+62611014 Msa0742650:CDS
AATACGAAAATTAATGGTGA+AGG 0.560690 5_1:+62610882 Msa0742650:CDS
TTTGAGTATCGTATATATGG+TGG 0.571975 5_1:-62610983 None:intergenic
TTAAAGAGATCACTTCATTG+AGG 0.591642 5_1:+62611533 Msa0742650:CDS
CACTTCATTGAGGAAGAATG+TGG 0.608657 5_1:+62611543 Msa0742650:CDS
CATGGTTAATCCAACAGAGG+AGG 0.638279 5_1:+62611032 Msa0742650:CDS
GCCAAATACAACAAATACAG+TGG 0.645246 5_1:-62610959 None:intergenic
TGGCATGGTTAATCCAACAG+AGG 0.731698 5_1:+62611029 Msa0742650:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATATTATATTATCTAC+TGG + chr5_1:62611868-62611887 Msa0742650:three_prime_UTR 10.0%
!!! TAATTTAGATTGTTTTTCAA+AGG + chr5_1:62611207-62611226 Msa0742650:intron 15.0%
!!! TAAAAAAACGTTTGATAATA+TGG - chr5_1:62611799-62611818 None:intergenic 15.0%
!!! TAATTTAGATTGTTTTTCAA+AGG + chr5_1:62611207-62611226 Msa0742650:intron 15.0%
!!! TAAAAAAACGTTTGATAATA+TGG - chr5_1:62611799-62611818 None:intergenic 15.0%
!! TACAAAATAAGAGTTTGATT+TGG + chr5_1:62610729-62610748 Msa0742650:five_prime_UTR 20.0%
!! AAATCCAATACGAAAATTAA+TGG + chr5_1:62610876-62610895 Msa0742650:CDS 20.0%
!!! GCTATTGTTTTTATGATTAA+TGG - chr5_1:62610937-62610956 None:intergenic 20.0%
!!! CTAGTATTGAAAGAAAAATA+AGG - chr5_1:62611118-62611137 None:intergenic 20.0%
!! ATTAAAACTAAGACATCAAA+TGG + chr5_1:62611361-62611380 Msa0742650:intron 20.0%
!! TTTCCATATCCTATAAAATA+TGG + chr5_1:62611449-62611468 Msa0742650:intron 20.0%
!! TTCCATATCCTATAAAATAT+GGG + chr5_1:62611450-62611469 Msa0742650:intron 20.0%
!! GAAGAAAAAGGATAATTAAA+TGG + chr5_1:62611630-62611649 Msa0742650:exon 20.0%
!! TACAAAATAAGAGTTTGATT+TGG + chr5_1:62610729-62610748 Msa0742650:five_prime_UTR 20.0%
!! AAATCCAATACGAAAATTAA+TGG + chr5_1:62610876-62610895 Msa0742650:CDS 20.0%
!!! GCTATTGTTTTTATGATTAA+TGG - chr5_1:62610937-62610956 None:intergenic 20.0%
!!! CTAGTATTGAAAGAAAAATA+AGG - chr5_1:62611118-62611137 None:intergenic 20.0%
!! ATTAAAACTAAGACATCAAA+TGG + chr5_1:62611361-62611380 Msa0742650:intron 20.0%
!! TTTCCATATCCTATAAAATA+TGG + chr5_1:62611449-62611468 Msa0742650:intron 20.0%
!! TTCCATATCCTATAAAATAT+GGG + chr5_1:62611450-62611469 Msa0742650:intron 20.0%
!! GAAGAAAAAGGATAATTAAA+TGG + chr5_1:62611630-62611649 Msa0742650:exon 20.0%
!!! AAAATAAGAGTTTGATTTGG+AGG + chr5_1:62610732-62610751 Msa0742650:five_prime_UTR 25.0%
!! TTCACCATTAATTTTCGTAT+TGG - chr5_1:62610883-62610902 None:intergenic 25.0%
! AATACGAAAATTAATGGTGA+AGG + chr5_1:62610882-62610901 Msa0742650:CDS 25.0%
!!! GATTTTGAGTATCGTATATA+TGG - chr5_1:62610989-62611008 None:intergenic 25.0%
!!! TTGCATGAATATGTCATTTT+AGG - chr5_1:62611332-62611351 None:intergenic 25.0%
!! TTCCCATATTTTATAGGATA+TGG - chr5_1:62611455-62611474 None:intergenic 25.0%
!! GCAATATTCCCATATTTTAT+AGG - chr5_1:62611461-62611480 None:intergenic 25.0%
!!! AAAATAAGAGTTTGATTTGG+AGG + chr5_1:62610732-62610751 Msa0742650:five_prime_UTR 25.0%
!! TTCACCATTAATTTTCGTAT+TGG - chr5_1:62610883-62610902 None:intergenic 25.0%
! AATACGAAAATTAATGGTGA+AGG + chr5_1:62610882-62610901 Msa0742650:CDS 25.0%
!!! GATTTTGAGTATCGTATATA+TGG - chr5_1:62610989-62611008 None:intergenic 25.0%
!!! TTGCATGAATATGTCATTTT+AGG - chr5_1:62611332-62611351 None:intergenic 25.0%
!! TTCCCATATTTTATAGGATA+TGG - chr5_1:62611455-62611474 None:intergenic 25.0%
!! GCAATATTCCCATATTTTAT+AGG - chr5_1:62611461-62611480 None:intergenic 25.0%
TTGTAGAACTACTTGAATGT+TGG - chr5_1:62610789-62610808 None:intergenic 30.0%
!! ACCACTGTATTTGTTGTATT+TGG + chr5_1:62610958-62610977 Msa0742650:CDS 30.0%
! TTTGAGTATCGTATATATGG+TGG - chr5_1:62610986-62611005 None:intergenic 30.0%
CAAAATCCATAAACGTTCAA+TGG + chr5_1:62611002-62611021 Msa0742650:CDS 30.0%
AAAATATGGGAATATTGCAC+AGG + chr5_1:62611463-62611482 Msa0742650:intron 30.0%
TTAAAGAGATCACTTCATTG+AGG + chr5_1:62611533-62611552 Msa0742650:CDS 30.0%
TGATATGCAATTCTCGATTT+GGG - chr5_1:62611765-62611784 None:intergenic 30.0%
TTGATATGCAATTCTCGATT+TGG - chr5_1:62611766-62611785 None:intergenic 30.0%
TTGTAGAACTACTTGAATGT+TGG - chr5_1:62610789-62610808 None:intergenic 30.0%
!! ACCACTGTATTTGTTGTATT+TGG + chr5_1:62610958-62610977 Msa0742650:CDS 30.0%
! TTTGAGTATCGTATATATGG+TGG - chr5_1:62610986-62611005 None:intergenic 30.0%
CAAAATCCATAAACGTTCAA+TGG + chr5_1:62611002-62611021 Msa0742650:CDS 30.0%
AAAATATGGGAATATTGCAC+AGG + chr5_1:62611463-62611482 Msa0742650:intron 30.0%
TTAAAGAGATCACTTCATTG+AGG + chr5_1:62611533-62611552 Msa0742650:CDS 30.0%
TGATATGCAATTCTCGATTT+GGG - chr5_1:62611765-62611784 None:intergenic 30.0%
TTGATATGCAATTCTCGATT+TGG - chr5_1:62611766-62611785 None:intergenic 30.0%
!! TACTTGAATGTTGGTTTCGA+TGG - chr5_1:62610780-62610799 None:intergenic 35.0%
GCCAAATACAACAAATACAG+TGG - chr5_1:62610962-62610981 None:intergenic 35.0%
CAACATCCATTGAACGTTTA+TGG - chr5_1:62611011-62611030 None:intergenic 35.0%
CATAAACGTTCAATGGATGT+TGG + chr5_1:62611009-62611028 Msa0742650:CDS 35.0%
!! TCTCTTAGAAGCATCTCTTA+TGG + chr5_1:62611071-62611090 Msa0742650:CDS 35.0%
!! CTCTTAGAAGCATCTCTTAT+GGG + chr5_1:62611072-62611091 Msa0742650:CDS 35.0%
CACAACAGATGCATTTATCA+TGG + chr5_1:62611154-62611173 Msa0742650:intron 35.0%
ACAGGTTTCTCTCTATTCTT+TGG + chr5_1:62611481-62611500 Msa0742650:intron 35.0%
! TCTTTGGATTGATGATCGAT+AGG + chr5_1:62611497-62611516 Msa0742650:CDS 35.0%
AGAGGAAACTGAGAAGAAAA+AGG + chr5_1:62611618-62611637 Msa0742650:CDS 35.0%
GTACACATACAGACATGTTT+AGG - chr5_1:62611682-62611701 None:intergenic 35.0%
!! TACTTGAATGTTGGTTTCGA+TGG - chr5_1:62610780-62610799 None:intergenic 35.0%
GCCAAATACAACAAATACAG+TGG - chr5_1:62610962-62610981 None:intergenic 35.0%
CAACATCCATTGAACGTTTA+TGG - chr5_1:62611011-62611030 None:intergenic 35.0%
CATAAACGTTCAATGGATGT+TGG + chr5_1:62611009-62611028 Msa0742650:CDS 35.0%
!! TCTCTTAGAAGCATCTCTTA+TGG + chr5_1:62611071-62611090 Msa0742650:CDS 35.0%
!! CTCTTAGAAGCATCTCTTAT+GGG + chr5_1:62611072-62611091 Msa0742650:CDS 35.0%
CACAACAGATGCATTTATCA+TGG + chr5_1:62611154-62611173 Msa0742650:intron 35.0%
ACAGGTTTCTCTCTATTCTT+TGG + chr5_1:62611481-62611500 Msa0742650:intron 35.0%
! TCTTTGGATTGATGATCGAT+AGG + chr5_1:62611497-62611516 Msa0742650:CDS 35.0%
AGAGGAAACTGAGAAGAAAA+AGG + chr5_1:62611618-62611637 Msa0742650:CDS 35.0%
GTACACATACAGACATGTTT+AGG - chr5_1:62611682-62611701 None:intergenic 35.0%
CACTTCATTGAGGAAGAATG+TGG + chr5_1:62611543-62611562 Msa0742650:CDS 40.0%
TCTATCTCTCTTCTCTTTGG+AGG - chr5_1:62611601-62611620 None:intergenic 40.0%
TCCAAAGAGAAGAGAGATAG+AGG + chr5_1:62611600-62611619 Msa0742650:CDS 40.0%
TCCTCTATCTCTCTTCTCTT+TGG - chr5_1:62611604-62611623 None:intergenic 40.0%
!! TTCTCGATTTGGGAGTTTCT+TGG - chr5_1:62611755-62611774 None:intergenic 40.0%
CACTTCATTGAGGAAGAATG+TGG + chr5_1:62611543-62611562 Msa0742650:CDS 40.0%
TCTATCTCTCTTCTCTTTGG+AGG - chr5_1:62611601-62611620 None:intergenic 40.0%
TCCAAAGAGAAGAGAGATAG+AGG + chr5_1:62611600-62611619 Msa0742650:CDS 40.0%
TCCTCTATCTCTCTTCTCTT+TGG - chr5_1:62611604-62611623 None:intergenic 40.0%
!! TTCTCGATTTGGGAGTTTCT+TGG - chr5_1:62611755-62611774 None:intergenic 40.0%
!!! GTGTTAGACCTTTTGAAGCG+TGG + chr5_1:62610904-62610923 Msa0742650:CDS 45.0%
!!! TAGACCTTTTGAAGCGTGGA+AGG + chr5_1:62610908-62610927 Msa0742650:CDS 45.0%
!!! AGACCTTTTGAAGCGTGGAA+GGG + chr5_1:62610909-62610928 Msa0742650:CDS 45.0%
ACGTTCAATGGATGTTGGCA+TGG + chr5_1:62611014-62611033 Msa0742650:CDS 45.0%
TGGCATGGTTAATCCAACAG+AGG + chr5_1:62611029-62611048 Msa0742650:CDS 45.0%
CATGGTTAATCCAACAGAGG+AGG + chr5_1:62611032-62611051 Msa0742650:CDS 45.0%
! TCCAACAGAGGAGGTTCTAA+TGG + chr5_1:62611041-62611060 Msa0742650:CDS 45.0%
!!! GTGTTAGACCTTTTGAAGCG+TGG + chr5_1:62610904-62610923 Msa0742650:CDS 45.0%
!!! TAGACCTTTTGAAGCGTGGA+AGG + chr5_1:62610908-62610927 Msa0742650:CDS 45.0%
!!! AGACCTTTTGAAGCGTGGAA+GGG + chr5_1:62610909-62610928 Msa0742650:CDS 45.0%
ACGTTCAATGGATGTTGGCA+TGG + chr5_1:62611014-62611033 Msa0742650:CDS 45.0%
TGGCATGGTTAATCCAACAG+AGG + chr5_1:62611029-62611048 Msa0742650:CDS 45.0%
CATGGTTAATCCAACAGAGG+AGG + chr5_1:62611032-62611051 Msa0742650:CDS 45.0%
! TCCAACAGAGGAGGTTCTAA+TGG + chr5_1:62611041-62611060 Msa0742650:CDS 45.0%
!!! GACCTTTTGAAGCGTGGAAG+GGG + chr5_1:62610910-62610929 Msa0742650:CDS 50.0%
GCCATTAGAACCTCCTCTGT+TGG - chr5_1:62611045-62611064 None:intergenic 50.0%
!!! GACCTTTTGAAGCGTGGAAG+GGG + chr5_1:62610910-62610929 Msa0742650:CDS 50.0%
GCCATTAGAACCTCCTCTGT+TGG - chr5_1:62611045-62611064 None:intergenic 50.0%
GACCCCTTCCACGCTTCAAA+AGG - chr5_1:62610915-62610934 None:intergenic 55.0%
GACCCCTTCCACGCTTCAAA+AGG - chr5_1:62610915-62610934 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 62610727 62611888 62610727 ID=Msa0742650;Name=Msa0742650
chr5_1 mRNA 62610727 62611888 62610727 ID=Msa0742650-mRNA-1;Parent=Msa0742650;Name=Msa0742650-mRNA-1;_AED=0.03;_eAED=0.03;_QI=84|1|1|1|0|0.5|2|240|148
chr5_1 exon 62610727 62611093 62610727 ID=Msa0742650-mRNA-1:exon:18413;Parent=Msa0742650-mRNA-1
chr5_1 exon 62611485 62611888 62611485 ID=Msa0742650-mRNA-1:exon:18414;Parent=Msa0742650-mRNA-1
chr5_1 five_prime_UTR 62610727 62610810 62610727 ID=Msa0742650-mRNA-1:five_prime_utr;Parent=Msa0742650-mRNA-1
chr5_1 CDS 62610811 62611093 62610811 ID=Msa0742650-mRNA-1:cds;Parent=Msa0742650-mRNA-1
chr5_1 CDS 62611485 62611648 62611485 ID=Msa0742650-mRNA-1:cds;Parent=Msa0742650-mRNA-1
chr5_1 three_prime_UTR 62611649 62611888 62611649 ID=Msa0742650-mRNA-1:three_prime_utr;Parent=Msa0742650-mRNA-1
Gene Sequence

>Msa0742650

ATGTTACAACTTTTATTTGCACTTTTGTCAATAGAATTAACAGTAATTCTAATACTATCTTTTGCAAATCCAATACGAAAATTAATGGTGAAGGTGTTAGACCTTTTGAAGCGTGGAAGGGGTCCATTAATCATAAAAACAATAGCAACCACTGTATTTGTTGTATTTGGCTCCACCATATATACGATACTCAAAATCCATAAACGTTCAATGGATGTTGGCATGGTTAATCCAACAGAGGAGGTTCTAATGGCACATCATCTCTTAGAAGCATCTCTTATGGGTTTCTCTCTATTCTTTGGATTGATGATCGATAGGCAACACTACTATATTAAAGAGATCACTTCATTGAGGAAGAATGTGGAAAAATCAAAGAAACAAAGCTACAATCATGAACCTCCAAAGAGAAGAGAGATAGAGGAAACTGAGAAGAAAAAGGATAATTAA

Protein sequence

>Msa0742650

MLQLLFALLSIELTVILILSFANPIRKLMVKVLDLLKRGRGPLIIKTIATTVFVVFGSTIYTILKIHKRSMDVGMVNPTEEVLMAHHLLEASLMGFSLFFGLMIDRQHYYIKEITSLRKNVEKSKKQSYNHEPPKRREIEETEKKKDN*